LOCUS       DARDHD010000001        77742 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000001 DARDHD010000000
VERSION     DARDHD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.001"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(113..1480)
                     /gene="nhaC"
                     /locus_tag="SDA06_000001"
     CDS             complement(113..1480)
                     /gene="nhaC"
                     /locus_tag="SDA06_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4227305.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1590..3011)
                     /locus_tag="SDA06_000002"
     CDS             complement(1590..3011)
                     /locus_tag="SDA06_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227306.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3254..5131)
                     /gene="tdc"
                     /locus_tag="SDA06_000003"
     CDS             complement(3254..5131)
                     /gene="tdc"
                     /locus_tag="SDA06_000003"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4227307.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5422..6678)
                     /locus_tag="SDA06_000004"
     CDS             complement(5422..6678)
                     /locus_tag="SDA06_000004"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG4227308.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6790..6995)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7258..8559
                     /locus_tag="SDA06_000005"
     CDS             7258..8559
                     /locus_tag="SDA06_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4227309.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8628..9227)
                     /locus_tag="SDA06_000006"
     CDS             complement(8628..9227)
                     /locus_tag="SDA06_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4227310.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9481..10362
                     /locus_tag="SDA06_000007"
     CDS             9481..10362
                     /locus_tag="SDA06_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227311.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10375..11985
                     /locus_tag="SDA06_000008"
     CDS             10375..11985
                     /locus_tag="SDA06_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG4227312.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12062..12721)
                     /locus_tag="SDA06_000009"
     CDS             complement(12062..12721)
                     /locus_tag="SDA06_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4227313.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13004..13669)
                     /locus_tag="SDA06_000010"
     CDS             complement(13004..13669)
                     /locus_tag="SDA06_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG4227314.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13816..14421)
                     /gene="dhaL"
                     /locus_tag="SDA06_000011"
     CDS             complement(13816..14421)
                     /gene="dhaL"
                     /locus_tag="SDA06_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG4227315.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14449..15459)
                     /gene="dhaK"
                     /locus_tag="SDA06_000012"
     CDS             complement(14449..15459)
                     /gene="dhaK"
                     /locus_tag="SDA06_000012"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG4227316.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15434..15880)
                     /gene="dhaM"
                     /locus_tag="SDA06_000013"
     CDS             complement(15434..15880)
                     /gene="dhaM"
                     /locus_tag="SDA06_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG4227317.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15983..16303
                     /locus_tag="SDA06_000014"
     CDS             15983..16303
                     /locus_tag="SDA06_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG4227318.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16415..17284)
                     /locus_tag="SDA06_000015"
     CDS             complement(16415..17284)
                     /locus_tag="SDA06_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4227319.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17380..18683)
                     /locus_tag="SDA06_000016"
                     /pseudo
     CDS             complement(17380..18683)
                     /locus_tag="SDA06_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(18680..19189)
                     /locus_tag="SDA06_000017"
     CDS             complement(18680..19189)
                     /locus_tag="SDA06_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227320.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19364..21499)
                     /locus_tag="SDA06_000018"
     CDS             complement(19364..21499)
                     /locus_tag="SDA06_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4227321.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21582..23237)
                     /locus_tag="SDA06_000019"
     CDS             complement(21582..23237)
                     /locus_tag="SDA06_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4227322.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23239..23553)
                     /locus_tag="SDA06_000020"
     CDS             complement(23239..23553)
                     /locus_tag="SDA06_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4227323.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23723..23983
                     /locus_tag="SDA06_000021"
     CDS             23723..23983
                     /locus_tag="SDA06_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG4227324.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23997..24311
                     /locus_tag="SDA06_000022"
     CDS             23997..24311
                     /locus_tag="SDA06_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG4227325.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(25041..25595)
                     /locus_tag="SDA06_000023"
     CDS             complement(25041..25595)
                     /locus_tag="SDA06_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG4227326.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(25721..27649)
                     /locus_tag="SDA06_000024"
     CDS             complement(25721..27649)
                     /locus_tag="SDA06_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4227327.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(27955..28671)
                     /locus_tag="SDA06_000025"
     CDS             complement(27955..28671)
                     /locus_tag="SDA06_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4227328.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(28672..30495)
                     /gene="glpO"
                     /locus_tag="SDA06_000026"
     CDS             complement(28672..30495)
                     /gene="glpO"
                     /locus_tag="SDA06_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG4227329.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(30509..32005)
                     /gene="glpK"
                     /locus_tag="SDA06_000027"
     CDS             complement(30509..32005)
                     /gene="glpK"
                     /locus_tag="SDA06_000027"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG4227330.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(32141..33601)
                     /locus_tag="SDA06_000028"
     CDS             complement(32141..33601)
                     /locus_tag="SDA06_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227331.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(33591..34886)
                     /locus_tag="SDA06_000029"
     CDS             complement(33591..34886)
                     /locus_tag="SDA06_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4227332.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(35218..35895)
                     /locus_tag="SDA06_000030"
     CDS             complement(35218..35895)
                     /locus_tag="SDA06_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG4227333.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(35925..37016)
                     /locus_tag="SDA06_000031"
     CDS             complement(35925..37016)
                     /locus_tag="SDA06_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG4227334.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(37117..37551)
                     /locus_tag="SDA06_000032"
     CDS             complement(37117..37551)
                     /locus_tag="SDA06_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4227335.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(38135..39592)
                     /gene="ascB"
                     /locus_tag="SDA06_000033"
     CDS             complement(38135..39592)
                     /gene="ascB"
                     /locus_tag="SDA06_000033"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4227336.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(39589..41484)
                     /locus_tag="SDA06_000034"
     CDS             complement(39589..41484)
                     /locus_tag="SDA06_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4227337.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(41542..43317)
                     /locus_tag="SDA06_000035"
     CDS             complement(41542..43317)
                     /locus_tag="SDA06_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4227338.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(43367..44863)
                     /locus_tag="SDA06_000036"
     CDS             complement(43367..44863)
                     /locus_tag="SDA06_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4227339.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(45057..46679)
                     /locus_tag="SDA06_000037"
     CDS             complement(45057..46679)
                     /locus_tag="SDA06_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4227340.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(47128..48441)
                     /locus_tag="SDA06_000038"
     CDS             complement(47128..48441)
                     /locus_tag="SDA06_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4227341.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(48686..48910)
                     /locus_tag="SDA06_000039"
     CDS             complement(48686..48910)
                     /locus_tag="SDA06_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227342.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(48930..49352)
                     /locus_tag="SDA06_000040"
     CDS             complement(48930..49352)
                     /locus_tag="SDA06_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG4227343.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(49496..50206)
                     /locus_tag="SDA06_000041"
     CDS             complement(49496..50206)
                     /locus_tag="SDA06_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227344.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(50224..50721)
                     /locus_tag="SDA06_000042"
     CDS             complement(50224..50721)
                     /locus_tag="SDA06_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4227345.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(50758..51210)
                     /locus_tag="SDA06_000043"
     CDS             complement(50758..51210)
                     /locus_tag="SDA06_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4227346.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(51345..51950)
                     /locus_tag="SDA06_000044"
     CDS             complement(51345..51950)
                     /locus_tag="SDA06_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4227347.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(52400..53020)
                     /locus_tag="SDA06_000045"
     CDS             complement(52400..53020)
                     /locus_tag="SDA06_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4227348.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(53022..53891)
                     /locus_tag="SDA06_000046"
     CDS             complement(53022..53891)
                     /locus_tag="SDA06_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG4227349.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(53914..54321)
                     /locus_tag="SDA06_000047"
     CDS             complement(53914..54321)
                     /locus_tag="SDA06_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4227350.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(54340..55629)
                     /locus_tag="SDA06_000048"
     CDS             complement(54340..55629)
                     /locus_tag="SDA06_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4227351.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(55656..55958)
                     /locus_tag="SDA06_000049"
     CDS             complement(55656..55958)
                     /locus_tag="SDA06_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4227352.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(55983..58076)
                     /locus_tag="SDA06_000050"
     CDS             complement(55983..58076)
                     /locus_tag="SDA06_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4227353.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(58565..59329)
                     /locus_tag="SDA06_000051"
     CDS             complement(58565..59329)
                     /locus_tag="SDA06_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG4227354.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(59440..60309)
                     /gene="mdcB"
                     /locus_tag="SDA06_000052"
     CDS             complement(59440..60309)
                     /gene="mdcB"
                     /locus_tag="SDA06_000052"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG4227355.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(60334..61410)
                     /locus_tag="SDA06_000053"
     CDS             complement(60334..61410)
                     /locus_tag="SDA06_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG4227356.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(61434..62027)
                     /locus_tag="SDA06_000054"
     CDS             complement(61434..62027)
                     /locus_tag="SDA06_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG4227357.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(62031..63707)
                     /gene="mdcD"
                     /locus_tag="SDA06_000055"
     CDS             complement(62031..63707)
                     /gene="mdcD"
                     /locus_tag="SDA06_000055"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG4227358.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(63697..63996)
                     /locus_tag="SDA06_000056"
     CDS             complement(63697..63996)
                     /locus_tag="SDA06_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG4227359.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(64098..65744)
                     /gene="mdcA"
                     /locus_tag="SDA06_000057"
     CDS             complement(64098..65744)
                     /gene="mdcA"
                     /locus_tag="SDA06_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG4227360.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(65751..66662)
                     /locus_tag="SDA06_000058"
     CDS             complement(65751..66662)
                     /locus_tag="SDA06_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4227361.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            66782..67639
                     /locus_tag="SDA06_000059"
     CDS             66782..67639
                     /locus_tag="SDA06_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4227362.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(67826..68188)
                     /locus_tag="SDA06_000060"
     CDS             complement(67826..68188)
                     /locus_tag="SDA06_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG4227363.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            68534..69973
                     /locus_tag="SDA06_000061"
     CDS             68534..69973
                     /locus_tag="SDA06_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227364.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(69980..72532)
                     /locus_tag="SDA06_000062"
     CDS             complement(69980..72532)
                     /locus_tag="SDA06_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG4227365.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(72646..73977)
                     /locus_tag="SDA06_000063"
     CDS             complement(72646..73977)
                     /locus_tag="SDA06_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227366.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(73943..75421)
                     /locus_tag="SDA06_000064"
     CDS             complement(73943..75421)
                     /locus_tag="SDA06_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227367.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(75726..77561)
                     /gene="tdc"
                     /locus_tag="SDA06_000065"
     CDS             complement(75726..77561)
                     /gene="tdc"
                     /locus_tag="SDA06_000065"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4227368.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        22284 a        16511 c        13358 g        25589 t
ORIGIN      
        1 aactccttcg gaaataagct gaaattcaca aaaatttaaa aagcaatttt cttgagtttc
       61 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttccttc cttcaatttt
      121 ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt aaagacaggt gcaagaatcg
      181 agaaaaagac gaatggcaag taatgcaacg cgctaacttt catcgttccc ataatgaatg
      241 tgccgctgac tccccacggt acgagagaat tgacggtcgc acctgaatcc gctagtgttc
      301 gtgtcaaata ggtttttgat aagccaacgt catcaaaaga acgcttgaat gtttctcctg
      361 gaagaatgat ggacagatac tgttctccaa cgatcagatt gattccgata cagctcaagg
      421 cagtaaaccc gactaaacga gaaggtcggt ccatcttttc ttttaattct tgaacgatgg
      481 tatcaacgat cagatatttg atcagtaacc cacctaatgc caatgcaagt aaaatcaaac
      541 tagcagaacc taacatactc atgatcccgc ctcgagaaag cagttcatct agctttggat
      601 caggtgtatt tgcgacataa ccattcatca agatgtctgc tgcttttccg accgtcaaac
      661 ttttctcatg gataaaagaa agaacgagag cagctgtcga tcccgctagt aatgtaggga
      721 tagccggaat ttttttgatt gcccccccaa ataaaataac tagtggaatc aatgtccaag
      781 gagagatcca aaagctcgct ttcatgtttt ggatcatttc attgatacta gctagatcgg
      841 ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg aatgctggga
      901 aatccgtata acgcatacca gcgatatgtt cgaatagatt aactcctcca atacccgctg
      961 ccaagttggt cgtatctgat aaaggcgagc agttattccc taaaaaggca ccggaaacga
     1021 ctgcgcctgt agtcattgca tcattgaatc caagtaaatg accgatcccc aaaaaagcaa
     1081 ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca attgcacaaa
     1141 caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca taaaccataa
     1201 tcgtagggat ggttccagat aaaatccatg aagcaactaa gacaccaatc aaaagaaaaa
     1261 tcaggattgg aatgatccct ggagtgattc ctttgatgat cccgtcatgg atctcatccc
     1321 atgtgaactt tttaaatctg ccataaaaca acaaacacat gaaagctgca aggatcggga
     1381 tatgtggcga gagctggaat ccgatgatca gtactcctag gagtgccaac aacacgatca
     1441 atacagcgat actttctttt aaagaaatat ttttattcat cgtcgatctc ctatttcaaa
     1501 tgaaaaggcc ctgccatgaa aaaactattc acttggcagg acctcctact aaatcagcga
     1561 caaaacgtcg gctgaaagac ttattttttt tatttttctt ctaaactttt ttctacctca
     1621 atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact
     1681 gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg
     1741 acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt
     1801 ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa
     1861 acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag
     1921 aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt
     1981 gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat
     2041 tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc
     2101 caagctgata attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc
     2161 acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat
     2221 tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa
     2281 actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa
     2341 cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag
     2401 tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct
     2461 agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg
     2521 atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata
     2581 tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg
     2641 aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa
     2701 gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca
     2761 aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga
     2821 agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca
     2881 aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc
     2941 gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca
     3001 gatttactca ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag
     3061 tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt tgagtctatt
     3121 gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc
     3181 ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac
     3241 atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca
     3301 atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt
     3361 tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt
     3421 cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga
     3481 tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg
     3541 aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt
     3601 tttctttgaa tacatagtct accatattga aatctggata agttaatgga tgaacttcga
     3661 tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac
     3721 cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt
     3781 gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc
     3841 caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa
     3901 cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca
     3961 ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta
     4021 cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt
     4081 ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc
     4141 cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac
     4201 gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac
     4261 cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt
     4321 cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga
     4381 taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt
     4441 ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt
     4501 tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc
     4561 aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt
     4621 tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt
     4681 ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca
     4741 tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa
     4801 aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc
     4861 cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat
     4921 cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa
     4981 taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat
     5041 tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta
     5101 ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa
     5161 ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat
     5221 ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta
     5281 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
     5341 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc
     5401 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
     5461 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
     5521 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
     5581 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
     5641 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
     5701 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
     5761 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
     5821 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
     5881 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
     5941 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
     6001 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctactt ctggtccttc
     6061 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
     6121 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcagaatttg
     6181 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
     6241 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
     6301 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
     6361 tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac
     6421 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
     6481 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
     6541 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
     6601 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
     6661 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
     6721 gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
     6781 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
     6841 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
     6901 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
     6961 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
     7021 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
     7081 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
     7141 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
     7201 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
     7261 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
     7321 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
     7381 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
     7441 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
     7501 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
     7561 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
     7621 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
     7681 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
     7741 ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
     7801 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
     7861 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
     7921 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
     7981 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
     8041 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
     8101 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
     8161 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
     8221 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
     8281 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
     8341 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
     8401 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
     8461 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
     8521 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca
     8581 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
     8641 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
     8701 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
     8761 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
     8821 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
     8881 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
     8941 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
     9001 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
     9061 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
     9121 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
     9181 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
     9241 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
     9301 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
     9361 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
     9421 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
     9481 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
     9541 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
     9601 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
     9661 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
     9721 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
     9781 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
     9841 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
     9901 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
     9961 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    10021 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    10081 gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac tcgatgaatt gcgtcatttg
    10141 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    10201 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    10261 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    10321 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    10381 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    10441 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    10501 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    10561 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    10621 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    10681 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    10741 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    10801 cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcagattc gatcactgca
    10861 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    10921 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    10981 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    11041 atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt
    11101 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    11161 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    11221 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    11281 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    11341 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    11401 gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca
    11461 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    11521 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    11581 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    11641 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    11701 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatgggt
    11761 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    11821 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    11881 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    11941 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    12001 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    12061 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    12121 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    12181 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    12241 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    12301 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    12361 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    12421 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    12481 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    12541 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    12601 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    12661 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    12721 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    12781 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    12841 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    12901 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    12961 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    13021 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    13081 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    13141 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    13201 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    13261 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    13321 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    13381 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    13441 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    13501 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    13561 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    13621 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    13681 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    13741 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    13801 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    13861 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    13921 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    13981 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    14041 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    14101 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    14161 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    14221 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    14281 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    14341 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    14401 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    14461 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    14521 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    14581 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    14641 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    14701 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    14761 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    14821 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    14881 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    14941 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    15001 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    15061 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    15121 attcttaacg atcagcaaaa ctccttttcc actatccgct tccttaatag ccgtctgaat
    15181 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    15241 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    15301 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    15361 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    15421 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    15481 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    15541 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    15601 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    15661 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    15721 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    15781 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    15841 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    15901 taataaattt ctaattttgg gaagatatgt gtagtgcttc ttgaagtttg ttatacttta
    15961 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    16021 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    16081 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    16141 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    16201 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    16261 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    16321 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    16381 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    16441 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    16501 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    16561 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    16621 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    16681 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    16741 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    16801 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    16861 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    16921 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    16981 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    17041 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    17101 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatagttg
    17161 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    17221 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    17281 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    17341 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    17401 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    17461 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    17521 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    17581 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    17641 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    17701 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    17761 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    17821 ttttgcccaa cgttttcttt gtcgatatag catcttcaac gtcacaggta cttccatgaa
    17881 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    17941 actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    18001 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    18061 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    18121 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    18181 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    18241 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    18301 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    18361 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    18421 attcaatttc gtcatcaaat actcgattgt atcttcgatc acaatttctt cattatgggc
    18481 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    18541 cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    18601 aatcggataa gaaacgagaa aaaatacata atagacaaac aagtatctaa gaataaatta
    18661 cctgtatggg tgattgagat catttcagtt ccaccccatt ttctttatac aaatctgcaa
    18721 tgaatgtcgt atctagattt ttctcttcgg agacagaata aaaacggacc gtttctctct
    18781 cttcttttgt tccaaaacgt tccgtataca cttcttcaag taattcttgt ctttttttca
    18841 atttttcatc ttcatattca aaagatttat ttactttttt gtatagattt cgattgttcc
    18901 acctagtcaa tacaaatgag gtaatcagga aactgaaaaa aatcaataca aaaaagccga
    18961 ttaagaaata cagcattttc ttttctgata aaaaagcgta caaggaaaaa tccttggctc
    19021 tttctggtct cgttaatggg aaaaagaccc atataaaagg aagaacaacg atcatccatc
    19081 ctaaaagagc cactaacgtt tgccaaatct ttaaccagta attacctttt gaaaaatact
    19141 tatctttcac caaaattgat gcaacttctt ctcttgttac ttttttcata tcctcacctc
    19201 acgttttttt gttacgacgt aggagtttac catacatttc atttcttttc acataaagtg
    19261 ttcctaaatt tttcctaaga aaaagaacaa aacatttctc atcagcaaat gaaaaatatt
    19321 ttgttctttt tatcagttgt tctttttatt ttttatctct taattatgtt gctctttcca
    19381 atacgacaat gcttttcatt cctctagata tcgccgtgta catttgtctg cgtcttcttt
    19441 gctcatctcc atcgttttca aatggatata agattacatg atcaaattcc agtccttttg
    19501 ctagactcaa aggaatgatt tggcagtttt ctaatccata tgaatttttt ttaatcgatt
    19561 cttgtagcgt ttgggtttcc tcttctgttt gggtgataac tgccgctttt tctcctttta
    19621 gagaaaccaa aatctcagct agtattttca acaattcttc agaggatcga aaagagcggt
    19681 attcgggttc ttttccttgc ggtcggatcg atacgatgtc gatcttctct tgatccacag
    19741 cataggtttt gaaaagctct gtgattgctc cgctagaacg gtaactattt ctaagatcat
    19801 accgatggat cggcagatgg tagtcgtcga aacaacgcat aatatcagca aaagtagtgg
    19861 aagaattaaa gattgcctga ttctcgtcac ctactaaagt aaagtgagtt tttggaaata
    19921 attcgatcaa tagacctaat tgtgccaggg tataatcttg tacttcatca atcaatagat
    19981 agcgaaatgc tggtacattt accttttcaa caagtaaatg acgaatagct aatataatga
    20041 ctgcttcatc aacagtacgt gtgggttgcc aagcataagc tgagccatat cgtttttcat
    20101 aaattctttc aaacaactga tgctcatcca cccatgccat ttcttcgatt tgtctggtta
    20161 tttttcgata ttttttttgt aaaagtttcc ttgcataagc tacaatactt tgttccgaat
    20221 catcagaaat caatttaccg aataattcca actgcctatc ttctgtcaac gagagtacct
    20281 gattctgcac agcaggactt tttgcttgtt tcaaaagatg attcttccat tcgctcatca
    20341 gtgccttttt cgttgcttga agcctatcga tcaatttagg atgagcagga actttctcat
    20401 atattttttc gatcgttttt ttagaaagga ctgtttttcc atttttagta agtccacgga
    20461 taaaattcgg atgatctaaa aacaaaggat ctgattgttt tagattatcg ataaactttt
    20521 tgctacgcaa acgttctgtc tgctccgata cattttctcc cgtgatacgt ttgaaataag
    20581 cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac cagctgtatg atggttagat
    20641 tcagtggatt tctttcacct aatgatggta ggacattcga aatatagtcg ataaaccgat
    20701 gattaggtga tagaatcagc aagtcatcgg atgtcatcgt atctcgatat aaatagagca
    20761 gatacgcaat acgctgcatg atagcagagg ttttaccact tcctgctacg ccatttacca
    20821 agatattttg acttttcaca tcccgaatga ttgtgttttg ttccgattgg atcgtagcag
    20881 tgatagcctt catttcattg gtctcatctt ggcctaagac ttctaataat acgtcatctt
    20941 gaatagctac agacgtatca aaaaacttga ccaaacgatc tctttccaaa agaaattgtc
    21001 gtctttcttt aataaccgta tcgattttat tttgacgaac cgtataagaa gatggaccaa
    21061 tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca atcataaaca agattttcat
    21121 ctgcatcatc tgtgaaattt gctgtaccga tataaaagga ttcttttggc tcattatcca
    21181 gaaagtctac aacgactttt ccaaagtagg gacttttcaa taaacgttcg atctttgcta
    21241 gctgattttc tgcgtttttc tgtttgatat tcatctgatc gatttctcta tttttcattt
    21301 caatcatcga aaaagtatcc aggttatcta agtaactgtc aaaattcaat cgaagctcgc
    21361 cagccatttc tttcaacgac tggtttcctt ctatttttgc cgtatccatc gattcccgca
    21421 gttcgttttt tttatcaatt agcttttgat acacgttctt cagatatgct tcttctaact
    21481 gtttttgtgt gttgaccata cacattctcc tcttttttta aagtggtgga accgattagt
    21541 ttacctcttt taagagtcga atgcaagctt tttaacgagt atcatttgtt aggatatatc
    21601 agttcttcag gtcggacagt ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg
    21661 tagccagact gcttcaagat tctttccgct agatagctac gtaatccgct atgacagctg
    21721 acgatatatt cttgttggct gtctaattca tttaatcggt cccgaagttc attcaaaggg
    21781 atatgtttgg cttccttgaa tttgcccttt tccaattctt ctgctgttcg cacatctaat
    21841 aaaatttttc cttttgctag ttctgtcggc aattcatgcc attgtacggt ttcactaagc
    21901 ccttctgcaa tgttcattgc tgcatatcct agcatgttta ctggatcttt ggctgagcca
    21961 aatggcggtg catacgtcaa ttctaattcc ggtaaatcga agatcgtcaa atgccctttg
    22021 attgctgttg ctaaaatatc gatccgttta tccacacctt ttgccccaac accttgtgca
    22081 ccataaatct cgccagtctc tggatggaag atcaacttca gtaaaatatc tgttgcttct
    22141 ggataatagc ttgcatgatc ttttcctgat acatgaatga cttcaaaaga cagttcgttt
    22201 tctttggctg ttctttcact taaaccggtg gatgcggcag tcattccaaa cgttcgtata
    22261 attgctgttc caataccgcc ttgatttctt cgagaaattc cggaaatcgt gtccgcaact
    22321 tgtctacctt gacgattggc tggtgaagca agagaaataa gtgcatcttt tccagtgatt
    22381 tgctgtttta caacgatagc atcccccacc gcataaatat caggaatatt cgtttgataa
    22441 cgttcgtcta ctaggatacc cccgcgtatg cctaattcga ttccagcggt cactgctagc
    22501 tggttatcag gacgaacacc aatggataaa ataactaaat cagcagggat ggtacgccca
    22561 tcatccagtc gcaatcgttt cccgtggtct tcaaatccag cgacagcatg agaagtaatc
    22621 acttctactc cttgatgaga caattctttt tctataaaag ctgccatttc ttcatccagt
    22681 gttggaagaa tatgtggtgc ttgttcgatc accatgactt ctaaacctct tctttttagg
    22741 ttttctgcca tttccaatcc gatgaatcct gcaccaacga tcacggcacg ctttggctgt
    22801 ttttctaatg catgcatcac tttatcgata tctggtacat ttctaataga aaaaacattg
    22861 tcggcttctg ttatccctgg aatcgatgga acaactggtt ttgcaccagg agataaaacc
    22921 aatgcatcgt actgttcaat gtgtttttga ccctcatgta cgacttctac cgttttgttt
    22981 tcgggaaaga tggctgtcac ttcatgattt ggacgcacat ctaaacaaaa acgagcggat
    23041 aatgattctg gcgtttggac aagaagattt tcccgttcac tgatttctcc cgaaagataa
    23101 taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt tttcaaaaac aacgatttct
    23161 gcatcttcca ttaatcggcg aagccgtgta gcagcagaca ttccacctgc tacacctccg
    23221 acaataacga ttttcatctt atttccctcc tactgttcga tcaaaccatt gattcatgcc
    23281 accacgtaca ttcactacat cataaccaga tttctttaat tctttagctg cttgtttact
    23341 cctcatccct gattgacaaa tcacatagac ggtcttttct ttgtctccat catatctgtt
    23401 tatactttgt aatggaacat ttttagctcc ttttatatgc ccgccccggt attctgcagg
    23461 tgttctaaca tcaagtaacg ctggattttc caaaagcttt tcctttaatt ctgggactgt
    23521 tatcgatttt attgattgaa atagcaaatt cattttctgc acctcattta catttttata
    23581 aatacctcat ggggtatatt aaacaggagc tttattaata tgtcaaccag aatgactctt
    23641 catttttttc actacttttt atatttgcat aaaataccga taaggggtat tatctaatta
    23701 taaaaataga ggagaatgtg ttatggagat tgataataaa aatatcctga accgtttgaa
    23761 acgtacagag ggacaaattc gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat
    23821 ggatgtcatc actcaattaa gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat
    23881 cgttgcagat aatctgaaaa attgctttga gaaacctgat agtaatccag aagaacaagc
    23941 ggctaaacta gaacaagcga tcaagatgat tattaaaaag taaaggaatg atttcaatga
    24001 aagtcgtttt ccatattgat gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa
    24061 acctgataaa agcttctcca gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta
    24121 ccggttatct agactctgcc aatgcagaat tcttagattt gcaaggagtc gtttttcacg
    24181 cttgcgccaa tgctatgcgt gctaatcata tttcagaaag ttctttacct gaacaagtaa
    24241 tcgttgtacc agcaggtgtc cttgatttag ttgaactgca atcccaaggt tatgcttata
    24301 tcaagcctta gtcagtagag gttgcgacag aagtgatcag cttcaagaaa tttcggctta
    24361 tttttcgaag aagctacttc tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg
    24421 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttgggc
    24481 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgg
    24541 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
    24601 gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc
    24661 gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt tttgcataaa
    24721 tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc
    24781 agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt
    24841 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc
    24901 taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt
    24961 tggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg
    25021 acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac
    25081 aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc
    25141 gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct
    25201 actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca
    25261 gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt
    25321 cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca
    25381 agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa
    25441 tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca
    25501 aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt
    25561 tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact
    25621 cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt
    25681 ttctttacag ccactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt
    25741 ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt
    25801 ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt
    25861 ctaaatcgta acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac
    25921 gaggcacgtg gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt
    25981 cataaactaa cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga
    26041 gctggcgttc ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt
    26101 ttttctgtgt ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca
    26161 tcgacatatc cattttatga gcaatccaca aagcttggct ttctcctgtt tctccaaccc
    26221 gcaactgata tttaggacga agtgcttccc gatcgaaagc catagcagcc ggcacaaaat
    26281 cttcatgatc tccggcgaat ttcttgattt ctccataatg ggtcgtggca atgatcaatg
    26341 ccccttgctc atacatagat tccatgattg cgatcgctag tgcagcccct tcatttggct
    26401 ctgttccact gccaatctca tctaaaagta ccaacgtatt cctttttatc tttttcaaaa
    26461 tagcagcgat gttttgcata tgaccagaaa atgtactcaa cgcattttct aaattttggt
    26521 ggtcaccgac atcgacgaaa atctcatcaa agacagcaag ctctgtccct tcttttgccg
    26581 gaacttgcat accgaacatc gccatcaaag tcaataaacc gactgtcttc aacacgactg
    26641 ttttgcctcc ggcatttgct ccggtgataa tcaagcctcg atagtcttta ccgatttcaa
    26701 catccaatgg taccgcatgc tcaggtaaaa atggatgtct gccttgcttg ataatgatat
    26761 gttccgattt attgattcta ggtgtgcttc cattgatttc tcggctaaat tttgcccgag
    26821 caaagatgat atctaacacc gtgatcgttt cgatcaataa atcgatcact tcttcgtttt
    26881 ctgcgatagc accagtcaat gccgccagga cttgatattc ttcactgatt tcttcagctt
    26941 ttaacaactg atagtgttca ttcaatttag aaatcacagt tggttcaata aaaaccgtcg
    27001 ttcttttatt tgattcatcg ataatcgttc catctatttt atttttatag ctggctttga
    27061 taggaatcgt atagtattcg cctttttgaa caatcattgc ttcttggatc atttctttgt
    27121 ttttcggatg acgcaagaat ttcagtaatt ttgactggat ttctttttcg attagttgta
    27181 attgtttgcg tactttacgc aaattacgtg acgcgtcgtc actgacttta ttatttttta
    27241 ttttctggtc aatcaactct tctacattta tcaaatcagg aagatgtttg ctataagcaa
    27301 acaacaaggg tgtctggtat tgattcttat caaaaaactt tgtgatcatc cgactgcttc
    27361 taagaaaatc cgcatattcg atcaaatcgg ttggctgcag aatcaaccct tttttgactt
    27421 gttctgtcag tgcatcgatc ctcggcaacc ccataaatgg aacatgctga ctgctttcta
    27481 agatcaagcg tgcttctctt gtttcttctt gacgatccaa gacagtttgt aggtttgtcg
    27541 agaccgtcat atcgctgatt cgttttttgc tatagtttcc aattgctctt gcttggattt
    27601 cctttttgat ccgatcaaat tgtaattgac taatcatttc gttgtccata ttctctcctt
    27661 ttcttgaaga gatacctgtc ccaaaataaa taagggccta gagataaata aaaggataga
    27721 acacagctta aaaaagtcat gtcctatcct tattagaata caaagatatt gttttcctga
    27781 caggtaccgt ttcataagca caccaaataa actttgttta ttggctattc gacttactga
    27841 ttagatacca gctacactca caaaacatac cccattcttc taggtcttat ttattttcca
    27901 cttaactata gcaagacaca ttttggttgt caaactttag tatgttttat agatttacac
    27961 cattaatgaa tagacaccta ccgcgataat tgctccgatc attggacctg cgattggtac
    28021 ccatgaatat gcccaatctg aagttccttt attagctatt ggcaataccg catgtgctaa
    28081 tcgaggacca aagtcccgag cagggttgat tgcatatcct gttggccctc ctaatgaaag
    28141 accgatggct aagatcaatc cacctacagc gaatacgttc gttccaggtg caaattcgtt
    28201 ttgtccaaaa gctagcaacc caagaactaa tacaaatgta ccaattaatt ctgtgatcac
    28261 atttgctgga taattccgta tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc
    28321 atctttcgtt gcattccaat gtggcaaata agaaagccaa acaagaatgg cccctgcaaa
    28381 agctcctaaa acttgcgcaa tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt
    28441 catcgccata gctagagaga ctgcaggatt caaatgagct gggcccatga atccagaaat
    28501 atagacagcc attgttacag cgagtcccca accaaatgcg atgacaatcc agccagaagc
    28561 ctgtgcttta cttttgttta aattcacagc cgcacacaca ccatctccaa gcaaaactag
    28621 gatcagtgtc ccaataaact ctcccatgat ttgagtcata tcactgttca tttatttctc
    28681 cccctttagt tcccgcagat ctgattctgc aatcaatgct tctaaacgtt tagtctgttg
    28741 cgccttctcc tcttctgtcc attcaaaata tccagcaatt gcatcgatca caggttgctt
    28801 gatctcatca agctgatctc gttcaaacaa cagatgattt gtacgacgaa tgagataatc
    28861 gcctggggcc aaaaccattt cttcttctaa accataacgt aaccgagctg actcagcgag
    28921 actcaaacca ggataaggtg ccatttcttt tgctaattca aagatacgtc gggcattagt
    28981 cccgtaaaaa tcagcgatat aagtagcatc ttcttcctct aaaccggctg ctactccttc
    29041 ttttgctaat tctgtcactg tttcttctaa tttagttgga tcgaagtttc cacctgaaat
    29101 ctgatatttt ttagagtcga tttctttcgt ctctattccg tattcttctt ttaataattg
    29161 gcgtatcaat cgcaaagctc cttctgccat cttacggtaa tcggtgattt tcccaccaga
    29221 taaagtcagc aaaccatctg gttctctttc taaagaacta cctctggaaa tcgtagaagg
    29281 tgctttttca tcacggctgt tttccaaatg gttcaacacg tcttctactt cagcacgaga
    29341 aactttgttt tccttatatt cacttactgt atcgacaact ttattgaagc tcttgtctga
    29401 aatcgatcca ttatctccac cattgtaatc agaaccagaa ttaccaatca acagtggacg
    29461 aagtcctgcc cagcttgctt cgatatcatt caatgtgata tttgcttctg gatagcgatg
    29521 attgatcaca tccaatagat aatctacatc ttcttgtgtg actttcggat ccgtaaaatc
    29581 tccttggtaa tccgtatctg tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa
    29641 aaccatccgc ccatcttgct ttcctgtatc gaagtatgtg ggttgcggta caggcagttt
    29701 tttcgcatct acaactaaat ggatcccttt agttggacgc attttaggag agactggacg
    29761 cgtaaaattc aagtttcgta ctttatctac ccaaggacca ctcgtattga tcactaattt
    29821 tgctttgatt tcgatcactt catctgtcag caggtcacga gctttgacgc caacgatttg
    29881 atcgccatca tataaaaagc caaccgcttt cattttactt actagataag ccccatcttc
    29941 ggcagccttt ttgatattat caatcactaa acgggcatca ttgttgcgga aatctagata
    30001 gacacccgcg ccttttagcc cttctttttt caaaaacggt tctctttcca atacttcttc
    30061 tggtgtcagc gtataattct catatttggt tcctgtcaca tttgccagtt tgtcataaag
    30121 gtccattgct actttgaccg agaacatatt gaaggttgtt gctccttcgc cctcatagat
    30181 tggcaaaagc attggatctg gttttgggat gtgtggggca atactttgta cgactgcacg
    30241 ttcaccaact gtgtcagcta ctacttccac atcaaatgtc ttcagataac gaataccgcc
    30301 atgaacaagt ttggtcgagc gagaggaagt cccttctgca aaatcctgca tttcgatcaa
    30361 tcctgttttg attcctgatg ctgctgactg gactgccaca ccggcaccag tgattccgcc
    30421 accgatgatc aacagatcca attcttcttg ctgcattttt tgaatgctat cttgtcttgt
    30481 cttgtttgaa aacatatgga tttcctcctt attcaaatgc ttgggtagct tttactgctt
    30541 ttttccaacc gttgtaaagt ttttctcggc gctcttcctc catttgaact tcaaatagct
    30601 tgccagcttc ataaaattcg cggatctcgt ctgtatcttt ccaataaccg acagctaatc
    30661 ctgctaaaaa tgctgcccct agcgccgttg tttccaagtt ttctgctcgt tggatcggta
    30721 cattcaagat atctgtttgg aattgcatca gatattcatt gtttgctgca ccgccatcta
    30781 ctttcaatac aggaatcttg attccagtgt catcttgcat cgtatcgatg atatctctca
    30841 cttgataagc aatcgactgt aaagtagctt taatgatatc ttcttttgtc gtaccacgtg
    30901 tcaagccaaa catagcccct cgtgctgcct gatcccaata aggagcacct agcccaacga
    30961 atgcaggaac aacataaacc tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat
    31021 cgctagcttt ttgcaacatt tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca
    31081 caaaaatact tccttctaaa gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg
    31141 tcaataaatt atttttggat aattgcggtt tttcaccggt attcatgaca ataaatgaac
    31201 ctgtaccata agtattcttg accatgcctg gttcgaatgc catttgtccg aataatgcgg
    31261 cttgctggtc tcctgccatc cctgaaattg gtgcttcgct tccataaaaa tgataacctt
    31321 gcgtataacc ataaacttct gagttagaag tgacttttgg taacatcact cgaggaatat
    31381 ttagtaaatc taaaatctcc tgatcccaat ctagctgatg gatattgaac aacatcgtcc
    31441 gactggcatt cgagtaatca gtaacatgtg cttgtccatc ggtcagtttc catactaacc
    31501 aagaatcgat cgttccaaac atcaattcgc ctttttcagc tcgttcttgc gcgccttcta
    31561 catgatcaag aatccagcgt atcttcgttg ctgagaaata ggcatcgatc actaaccctg
    31621 ttttttcgtg gaaaaattct tgatagcctt cattttttaa gttatctgca attccagctg
    31681 actgtctgga ttgccaaaca actgcatgat agattggacg tcctgtatct ttttcccata
    31741 ttactgttgt ttcacgctgg ttcgttatgc caatcccagc aatctgattt ggtttgatcc
    31801 ctgattcgat gaacgctccg gcaataactg actgtacaga attccagatt tcatttgcat
    31861 catgttccac ccaactttct tgcgggaaat attgcgtgaa ctctttttga gaactgccga
    31921 tgtgtcttcc tttcttgtca aagataattg ctcgtgagct agttgtacct tggtcaatcg
    31981 ccatgatata cgttgattct gtcattgtga ttccctccat tatatgcaaa cgctttcttt
    32041 aactactctt atcatactac tgttttaaaa aaagaaactg tcaaaatttt attctttttt
    32101 tctttttttg acagtttttt attatgaagc tattttttaa tcaattcttt tcttttttca
    32161 gcgtctctat tcttcttttc aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat
    32221 aatattcaca ctgttttttg tttctctggc aaaatgtgat caccaaatca tattctttac
    32281 cttcttcata acttccaatc tcaataccgc cgattcccct cagttctctg atcagatatt
    32341 gttgaaaagc aatacgataa atcggcagac tttctaaatc gattccaatc actactggtt
    32401 ttgcaatata aaaatcgatc atcaaaagaa cattggcata ctggatcatc aggtaactaa
    32461 gctcactatc ttcagctctt ttcctcggca attgctgcac cgctgtttcc tgtaatttcg
    32521 ccagtaactg tgttaaggaa tgcccgagca ttttctgctg ctgttccaaa agccgatcac
    32581 gctcatagac ttcgatcttt ccaacaaaga aaaaccattc attgttgatc tgggaaattt
    32641 ggtagcctac tgccttttct tctttgattt tcaaacgacg aggacgataa tgccataaca
    32701 tcgtttcccg aatgtaagta tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat
    32761 catactgata aaatatttct tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca
    32821 tcattgtttc gtatacattc agttctgctg ctgttcgact aagatgcatc acgatgattg
    32881 gatcgattgc ttgataaagc cggtccgact gatagagtaa tttcttttct ttcaaagttg
    32941 cgtacgtact tttttcattc aatagtcttt ttttcgtaat cccgagccag caagcaatct
    33001 gtgcttctgc tgatgccgta aacgttgttt ctaatacacg gtccaatcct agaatgaacg
    33061 gtcttttttc tggtgtattt tgcaaaaaaa gcggtcgctg atcttccggg atatattgga
    33121 atagttcata ataaaaatat cgtatctgta gctcttctcc gtataattga ccgtttttga
    33181 tttgtaatcc aaattcctct aagagctggt tcaattcttt gatctttcga aaaacagaag
    33241 actcactgat attaaacgct gtcgctaatc gaacgatgga tacttgctga tgttctaata
    33301 aatagacgag catcttaaac ttcaagcttt cctgcaataa aaacaataga attttttcca
    33361 aacttacatc ttcgtccaac acaagcagcg cctgatactc atttcgctgt acttctactt
    33421 ttttttccat cattcggcca atcaattcaa tatcttccaa atattgatca aacgaggctt
    33481 ttgacagttg gacatgctct ctcaaaccat tgatagaggc tgttccccct tgcagaacaa
    33541 ggtttcgcaa aatcaggatc tgtaactgct cttttctttc taataatgct tcaatcttca
    33601 taggcagtat tcgccacctt tgaccacacc atatctggca cacggtattc tggctggaaa
    33661 aatagctcat gttcttctaa ctgttcaaac gtccatcctt cttctactgc aattgtcagt
    33721 tggtcaagaa ggtggaacat ctcttttttg ctttggagct gtgctccaag aatctttttt
    33781 tcagaatcaa atagaaattt gagtttaact ttgctttgat ttattgctgt aagcttgtag
    33841 tctacgtatc ctgtttcaat tttttgggga taaaaaatgc tctcttcttc tgtcaaacct
    33901 acgcttccta agaaatagcc aaaccactga ttgccgactg gacgataggt tccctgatcc
    33961 tttgtctgtg agccactaat tgtcttgctg acatttcgtg ctgtacggac agcgttattt
    34021 actagagaaa catacatttt ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca
    34081 atcgcataaa cgtctttttg agacgtttgc aagtagtcat ctacccagat cgttccatca
    34141 tcattcaatg ttaattgttc ttcccacatc tgattgtcgg ggcgagaatg attagccagc
    34201 aaaacaagat catagttctc ctttcttttt tccgtctcta caatagcggt ttcattttcg
    34261 gtcaaagaca caacttgttc ttctaaaaaa agtgaaagcc cttgattttt catttccttt
    34321 actaacggtt ctgtcatttc aggatctaga taacgaaaaa gaattgactt tcggctttcg
    34381 taaagatgga tttgtttttt tcttgatgcc aacccttcag ctatttcgat tcctgcttgt
    34441 cctgcaccaa taattgcaat tttctttgca ttcatgatct tttgctgaag agccgttacg
    34501 ttttgtatct ttttaactgg gtgaataagc gttgattctg cagaaatatt tcgaaacttt
    34561 tgatttgaac cagtcgctag aatcaaacga tcaaaatcca atttttttcc tcctgctaaa
    34621 acgacttttt tttctttcaa tcctattact gtcttttctg tgtaaacatg gattcgatag
    34681 cggtcagtca gttcttcttt cgtgatccaa gaaagttgtt tttcactggt aacctttcct
    34741 tttaatatca aattgaaccc gctgggaatc catcctaact tcgattgctt ttcaatcaaa
    34801 tatatttcac ttgttggcat caattggcga agataaatag ccgcatgaat ccctgcaaac
    34861 gacccgccga caattacaat gttcatctgc attcctcttt cttataaggt gttctactgc
    34921 taacttctca atcagaaaac acagtattca taactcgctt tcaaccttat gatacatagc
    34981 gatgcagcag aatcaaccct ctccatcata ccgtgttcta ggtctgcttc tcaataatgt
    35041 actagtggac ctaattgaag agtatcattt ctgaagagat gtttcaaaaa atgtactctt
    35101 ataaaagaaa aaaactcgcc ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg
    35161 aaaagacgag ttttagcgtg tgtaatgtaa tagttagaat agacacatca tttattttta
    35221 attctttaat gcttcaacaa ctgcttcgcc taattgatga gcagattgag gattttgccc
    35281 tgtaagaagc cgcccatcaa tttgaaccac ttctgaaaaa gctgctccag actgatagtt
    35341 tgctcctttt tctttcaaag catcttctgc taagaatggt acttcattcg ttgtaccatt
    35401 tgcttcctct tcttcattac tgaacccagt cactgtacgt ccagctaaaa tagattttcc
    35461 agactcatct gtaactggca acaatcctac aacaccgtga caaacagctg agaccagccc
    35521 ttcatttcga tagatttgtg aagcgacttt cccaagatct attgcttctg gaaaatccca
    35581 cattgttccg tggccaccag cataataaac cgctgcataa tcatttgcat ccacatcatc
    35641 aggacgtaag gtatttccca aggcccgatt tctgaaatct tcatcttcat aataggccca
    35701 atcgatttca tccatattca aaacactttg cggttctaaa ggtacatatc cgcctttagg
    35761 actgacaaaa tctgcatcga tcccattttt tactaataca tcataaaaat gagtcagttc
    35821 acttaaccac aaacctgtag gacgctcgat cttttcgtac ttttctacat ttgttaatac
    35881 gatcaatact ttcattttcc catcctcttt ctataaaaat tgcgttattg ataacgtgct
    35941 ttcaagaaat tatataatgg gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt
    36001 ttcgttacaa attcatcctc acggttttcc aataatttct ctgcccagtc aggatcgacg
    36061 atcatttgct gtccaattgc tgcaagttct gcatatttca atacctgctc tacatcttcg
    36121 cgattgcgga tcccaccgac accaactaaa gggatctttc cttgaatcgt gttatggacc
    36181 acttcaagaa tcgatttatc gttgtattta tcagaacgag ctattcgatc atacatattt
    36241 aatgaaacat gcagataatc tagttttgac tcacgtaatc gttcaagaag ccagatcgtg
    36301 tcttcaaaac ggatacctgg tgtttcatat tcttctggtg agaaacgata ccctacgatg
    36361 aatggttttt tagcatattg ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata
    36421 aaacgataac gattttccaa gctaccgccc cattgatcct ctcgtctatt cgaatgcgga
    36481 gagaaaaatt gttggatcaa atacgtattt gctccatgca actcgattcc atcaaatcct
    36541 gattcaatcg ctctacgtgt ggctttgcca aatgcttgga ttgtttcaac gatttcttct
    36601 tcactcattg ctcgtggtgt ttccgcatca ggacgttttg ctgcaactgc acttgcagat
    36661 acaggctgtt ctccagataa agttgctcga cttgtcattc gtccaccgtg gaaaatctga
    36721 ataatcgctt ttgcgccttc tccttggatt gctttcgcta gctcacttaa tcgaggaatc
    36781 atttcatcat gagcgatgct gagttcacct ggccagcctt ttccgatatc ttggacattt
    36841 gcagcaccag taataacagc accaagacct ttagaacgtt gtttataata agtgatctca
    36901 tcttcggtga tcactccatt gaaaaagctg agttgtgtcg tcataggcga cagcatcaat
    36961 cggttcttca attgaactcc tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc
    37021 attcctccca taattcgctc tgattaacct atcgagaatc atctatcatt aaaccactcg
    37081 attagttgta catgcaacta atttgcttaa aaaattttat tttttttgta acttatctgt
    37141 tgcatcattc attgtctggg ctaattcttt acttgcttca tttcccaaaa ctgctttgta
    37201 tgactgatga agttcttccc agctaccatc gacttcctta gcaaattggc gtccttgttc
    37261 tgtaagataa actaatgaca agcgaccttc gctaactgtt ttgacgtatc ctttatattc
    37321 taatttttca acaaaacgag caatcgttga tggggcggaa tagagcattt gtgcaatatc
    37381 tttttgtggt attccatctt tttcttttaa tatcaataaa ataaaaccat aagttggtgc
    37441 cagacctgtc cttctaaacg cttcatcagc taatttattc aacatgcgat ctagcttttt
    37501 cacgctaaag aaaagacatt catctaacgc tttttcaatt ggcttttcca tttcatcccc
    37561 tccattggcg tacatatcat agtatgattt ttattttttg tcaaaaaaca cggaataatg
    37621 tccgcctcct ctgtctagat ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt
    37681 gttttcgaca aaactccttc tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag
    37741 ttttgagtat gaagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
    37801 ttaataccga aaaactggct tttgaacacc gtttatatca gaggttgtga aaaagccgtc
    37861 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttg tcgttttttg
    37921 agctaatgcc gatggtcagc tgattgtaca ccgtttattg agaggtcgtg agcctgccgt
    37981 tgaactccga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt atgactatgg
    38041 tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga
    38101 caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca tttgattgga
    38161 tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg ccattccctt
    38221 cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa gtagaagcgc
    38281 tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca tctttgatag
    38341 cttccttcat ctgctcaata tgctgtttca aatagtcaat gcggtaatcg tcttgaacgg
    38401 ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg aacagtggtt
    38461 tttgatacct atcatataat tgattcaaag caattctcaa acccttagga tcaatctgcc
    38521 agccccattc acttgatgga agatacggat ttttaattcc tccagttatt aagtttcctt
    38581 ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag tagctaaaag
    38641 aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct atttttaagt
    38701 tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct cttgcttgaa
    38761 catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata tcatccggat
    38821 cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt gaacctttga
    38881 taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg tgtgcacctt
    38941 ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg ctaacaaaag
    39001 ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact ttatttttat
    39061 agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc
    39121 atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga gaaacgactg
    39181 gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat aatcccgctt
    39241 cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata gacatccgat
    39301 agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga tgatagaaat
    39361 catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc gcttttactt
    39421 gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa tcgctgtcga
    39481 atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca ccttcacatt
    39541 gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc atatcgatcc
    39601 ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa tgatattttt
    39661 gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct ctttgattaa
    39721 ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc catcagatac
    39781 tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat ggatcagtat
    39841 ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac
    39901 ttccccgttt acaggactgt agatttttcc gtccgttgga atgatcgcaa acccttctcc
    39961 taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta tttcacctgc
    40021 tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat ccttgtagcc
    40081 gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag caactagcca
    40141 aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag caactgccaa
    40201 agcaccggct cctgcggcac cagctactcc accaaatata gctccacata tacaagcaat
    40261 gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag tgattccaaa
    40321 taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt tcgttttcaa
    40381 aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg ctggacctag
    40441 cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc cccaatgtaa
    40501 cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata aaacagggct
    40561 aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat ctgcaattgg
    40621 acctacaact aataaagtaa ttgggaccat cacgatgata caaatgaatg gtgtcaataa
    40681 atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag aaagaaccca
    40741 tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca cgggcatccc
    40801 tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat aaacgagagc
    40861 tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag cagtatatgc
    40921 caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa tgatataagt
    40981 tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc ctttgatcaa
    41041 accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa
    41101 agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat catcttctag
    41161 tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta ctacgacttg
    41221 aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta ccttctcctt
    41281 atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt
    41341 gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc
    41401 catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa gataagtata
    41461 gttcttagaa aacggtttct ccattcaatt agtacccctt ataatagcga acgaaactgt
    41521 acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa agaaaacaag
    41581 tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct tcctttgctg
    41641 atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt ggcttttgat
    41701 aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag aactaggaga
    41761 gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt cttctttgta
    41821 gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct tttcacgcaa
    41881 aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat aaatcaaatc
    41941 atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt ccgttatttt
    42001 gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga tccacatgtt
    42061 catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct ggttactaac
    42121 aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa
    42181 ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat
    42241 taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat tcatggtatt
    42301 atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa attctgtttc
    42361 atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct caaatgctct
    42421 tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat ttaactctaa
    42481 tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta gttctgatga
    42541 taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt ttgttttttc
    42601 taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc
    42661 ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt
    42721 cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt gttggatcac
    42781 cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt
    42841 tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa
    42901 aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt
    42961 ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa
    43021 atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc
    43081 agattcaatt gattctaaaa aataacctga acgtttattt gactggataa tgattttctt
    43141 ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa
    43201 agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat
    43261 ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca
    43321 ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag
    43381 atctatattc aaggttttta ccattagact ggataatttg tttataatat tcaaaactat
    43441 cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc
    43501 catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata
    43561 agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt
    43621 ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta
    43681 gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc aattcattca
    43741 tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct
    43801 tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt
    43861 ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca
    43921 agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt
    43981 aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt
    44041 taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg
    44101 cagaaaagct acacataata cccatcttag catttggatc gattttcttt aataattgag
    44161 tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta
    44221 gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat
    44281 aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt
    44341 ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt
    44401 gccacccccc atactctcct accaatgcta agggggtttc ataatgacta agagtaatta
    44461 ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag
    44521 cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc
    44581 gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa
    44641 aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct
    44701 ttttttcttc attccactga atactaggat gctggttcat gatcccgata agcgtatctg
    44761 tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg
    44821 ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct
    44881 atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt
    44941 tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac
    45001 aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat
    45061 tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt
    45121 ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaacagt gaacgtctgc
    45181 ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag
    45241 gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca
    45301 tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct
    45361 aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca
    45421 gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt
    45481 cctttcgcat tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg
    45541 ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc
    45601 gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag
    45661 atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca
    45721 attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat
    45781 gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat
    45841 ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac catggataat
    45901 tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg
    45961 ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca
    46021 tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc
    46081 acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc
    46141 tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc
    46201 agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc
    46261 aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga
    46321 ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc
    46381 accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt
    46441 tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg
    46501 tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc
    46561 agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct
    46621 tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg
    46681 attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct
    46741 cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa
    46801 acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg
    46861 gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat
    46921 tagctcaaaa aacggcaaaa tatgaggaac tattttttcg tttttttctc taatactcag
    46981 gccagaacac tgatttcaca acctctttat aaacggtgtt cactcagctg tccctcggta
    47041 ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca
    47101 ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac
    47161 aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa
    47221 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
    47281 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
    47341 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
    47401 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
    47461 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
    47521 tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc
    47581 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
    47641 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
    47701 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
    47761 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
    47821 taaaatcgtt gtagctgcta tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
    47881 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
    47941 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
    48001 agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
    48061 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
    48121 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
    48181 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
    48241 taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
    48301 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
    48361 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
    48421 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
    48481 gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata
    48541 agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt
    48601 tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa
    48661 agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct
    48721 gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac
    48781 ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg
    48841 ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca
    48901 ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca
    48961 aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt
    49021 tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa
    49081 gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg
    49141 cctaattcta attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg
    49201 attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg
    49261 tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt
    49321 gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg
    49381 tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact
    49441 ttttttacaa aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata
    49501 attctgcttc tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt
    49561 gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat
    49621 tgatggattc aatgaccagc accgctgctt gtgtttcttc cgtcagcatt gttctttggg
    49681 cttcacgcca gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt
    49741 ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg
    49801 cttttcctaa atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc
    49861 caaaagacaa ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc
    49921 gttcttgatt tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct
    49981 tgaatgtggt aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt
    50041 ctgtaaggta atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt
    50101 cactttcttt tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt
    50161 caggaaatac ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct
    50221 tgattagaat aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa
    50281 aacatgttcg ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt
    50341 gtgttcccaa acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc
    50401 tgtatctagt aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg
    50461 ctcgatttct aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt
    50521 caggtaaccc gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt
    50581 tagctcttct gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc
    50641 cggtgaatta tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa
    50701 gtctacatct gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca
    50761 atagatcctt ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac
    50821 tttatttaaa agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac
    50881 tgcattggag tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt
    50941 cggatagagt ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt
    51001 ggattctaat ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc
    51061 tagtttttct tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc
    51121 tcttgttaaa tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat
    51181 cattccgatg tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac
    51241 tagtttacct ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt
    51301 taataactag taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc
    51361 tggtttttgt tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt
    51421 ggcaactctt ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg
    51481 gcctcttcac cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga
    51541 atatcttttt gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat
    51601 cctgtttctt catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca
    51661 aatccaccag gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca
    51721 tcttttttga tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt
    51781 gtgagcgctt ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa
    51841 atcgtccgga tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca
    51901 gcttgagaaa gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct
    51961 tcttttttag caaatgtatg actatgtaat caagtataga agactggttt aattacggca
    52021 aataaatata atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga
    52081 cattcgactt ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata
    52141 agccccacca aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag
    52201 gcaggacagc tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc
    52261 acaagtcaca atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact
    52321 cggagctgaa cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg
    52381 atattaagcc atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata
    52441 gtggagtgta atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact
    52501 ggaatttgtc atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat
    52561 tggtcccttt cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc
    52621 tcaaatgacg atctgattca cgatcacgtt tgtaccgacg ttgacataac acttcaaagt
    52681 ctgctgttag gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc
    52741 gtgctttttg cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca
    52801 tatacaaatc aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc
    52861 gttcttccaa attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg
    52921 tgatggcata catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg
    52981 taccaggact accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta
    53041 attggacttt ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct
    53101 tcatttgctg gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt
    53161 tctttagcta ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc
    53221 ccgttattga tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact
    53281 agggggacat caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt
    53341 gatttgaatt gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct
    53401 tctacaaatt tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg
    53461 attccttctt ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca
    53521 tgggcatatt ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac
    53581 ccatcataca tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct
    53641 cgggcgtggt ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt
    53701 acattgacta acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg
    53761 atcggcgaac ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc
    53821 gtattaaaag ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat
    53881 gttgtataca tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg
    53941 ttttgcaatt tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc
    54001 accagcacga gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc
    54061 atctccgatc gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt
    54121 cacatacaat tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt
    54181 atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt
    54241 cgcttctggc gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt
    54301 tttcttaaaa aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg
    54361 gcttaagatc caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc
    54421 attgatgaat cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa
    54481 gaaaccatga gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa
    54541 tatccctgct gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa
    54601 gccgaacatg ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc
    54661 taaaatgaca gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc
    54721 atcaggaact aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag
    54781 acgtacacca gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc
    54841 aaacatgata tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa
    54901 aacagtgatg atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag
    54961 aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc
    55021 accaaaaagc ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa
    55081 agcaatatca tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc
    55141 aatggcgaaa caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc
    55201 ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa
    55261 aaaaatgtac ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa
    55321 gaacgtcagt gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga
    55381 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    55441 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    55501 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    55561 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    55621 aacgcccatg tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga
    55681 actggggtga ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc
    55741 gccgttttag gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg
    55801 atcacatctg gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct
    55861 actcctaatt tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa
    55921 cctgcaccgc atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt
    55981 tattatttct caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca
    56041 atcaatcgat ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc
    56101 agatgcgagt aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca
    56161 ggattaccga aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc
    56221 ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg
    56281 taatcattca ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta
    56341 accaatggtc tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc
    56401 atgatattgc tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt
    56461 tctaaattgt ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg
    56521 ccaccacttt ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg
    56581 ttaggctcta accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata
    56641 ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga
    56701 aaggtgctga aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt
    56761 aaaactgcag taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt
    56821 ccagtagcca tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc
    56881 ttgattacac tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt
    56941 atcttgccac caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt
    57001 gggaaataat ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg
    57061 attcttacaa tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt
    57121 agatggaaag gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat
    57181 tgagcttgta cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg
    57241 aaacgaagat aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg
    57301 tggtcaataa atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca
    57361 gagataaatt catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt
    57421 tttttataaa tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt
    57481 ttgatttcca ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca
    57541 tcttgattca taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc
    57601 gtacggatag atcgttctga acctttataa accagtcctt gctttccata actgacgatc
    57661 tctatcccat aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt
    57721 ttagagattt gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca
    57781 acggctaaaa ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct
    57841 tttatttcag tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaaactttg
    57901 ttgcgaattg tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga
    57961 acttcctgac ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt
    58021 actggatatt gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa
    58081 atgatctcct tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc
    58141 tgccacttga agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt
    58201 catctcctca acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa
    58261 aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag
    58321 tgcttcctat ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat
    58381 ttagtggaga ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa
    58441 atcgcttccc atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacact
    58501 gtttattcag aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt
    58561 cctgtcaatc cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag
    58621 aaaaatcaaa ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca
    58681 atcgcccatc ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa
    58741 aactattttc gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat
    58801 gatttcttac accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct
    58861 tgtctgtata acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct
    58921 tcatgaatat cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca
    58981 tgcgggacaa tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa
    59041 aagcagaaac ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac
    59101 tagaaggacc tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc
    59161 caaaataact gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc
    59221 tagatgtacc tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca
    59281 aacataggcc cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg
    59341 ttatctttta ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa
    59401 ccagatgcat aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg
    59461 acaaatgtat gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga
    59521 caagagatct gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc
    59581 tgtaaatgga cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc
    59641 cactaacgtt ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa
    59701 aactaacaac atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc
    59761 ttggattgca agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac
    59821 gccatatttt tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc
    59881 aggaaaagca gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc
    59941 catactgatt ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt
    60001 atgggtattg atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc
    60061 agcaattctt tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc
    60121 ttcaaaaaaa ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga
    60181 atgtgcaccc gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc
    60241 gatcaatgct tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc
    60301 atttatcatg acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc
    60361 tacccaaatg atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata
    60421 agctacaaga atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa
    60481 cagtacttgg aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca
    60541 tttgaacaat ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac
    60601 agcaaagatg atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc
    60661 aaagctcaag tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa
    60721 ggcaacaggt aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata
    60781 aacaaatgcg gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca
    60841 gctgtcatga taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata
    60901 taaaatcgtt gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta
    60961 accgagccaa acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca
    61021 taaatgaggc gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata
    61081 cggagtttgt tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata
    61141 cggaacgact agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact
    61201 tgtttttacc atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat
    61261 gacgtaaaag gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa
    61321 gctcaatatc gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac
    61381 gttgattttg atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa
    61441 agttctttgg ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct
    61501 ctcaaatact cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc
    61561 tcaaaaaaag caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg
    61621 tacagcagca aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca
    61681 aatcctaagc tgccgccgat tccccattta catcctgata atatgtttct agcagcatga
    61741 aattgctgat aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg
    61801 atgtcccacg gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc
    61861 tgattgcggg caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca
    61921 cgacgaactg ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct
    61981 tctatttcat gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc
    62041 cattcttcgt ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct
    62101 gtttcgtttg tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg
    62161 gcttcattga ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg
    62221 atatcttcta ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg
    62281 atagctggaa ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt
    62341 gcagcacttt ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag
    62401 cggcttgatt gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat
    62461 ccaataactg gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa
    62521 gccacatgga taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc
    62581 acaacgatga ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa
    62641 atttgtccca tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc
    62701 gggtttttag gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat
    62761 ttaccataat acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca
    62821 aaccaatcgt aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag
    62881 tcaattgatt ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt
    62941 tttgaaagat ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc
    63001 gtgtcttttc gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca
    63061 gtcactaatt catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa
    63121 atcaagtctt tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct
    63181 ggtccattca aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa
    63241 gtaatcgaca taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca
    63301 tattgtttta gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt
    63361 gcttcttgca aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct
    63421 tttttatttt cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca
    63481 ccgataccgc caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg
    63541 atcgtgccac ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt
    63601 aaacttggcg cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga
    63661 gcgttcactc gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc
    63721 cattgcttgc gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac
    63781 gatatctgct gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc
    63841 gccgaagcca tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa
    63901 catcaagatt tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg
    63961 ctgtttcgtt ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac
    64021 agagcataca cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa
    64081 ggtttttctc gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca
    64141 tctgaccatt cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca
    64201 ctacgtttga tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct
    64261 agtgattctt tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct
    64321 gcttgtcgtt cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg
    64381 atatgtgtag tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt
    64441 tgaacagcta gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt
    64501 tcaaccattt gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt
    64561 gattgccatg ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca
    64621 ccgccaaaac cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat
    64681 tgcaaggttg aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt
    64741 cggttactgc gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata
    64801 tatttttcca taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg
    64861 atggctggaa tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt
    64921 aaccctagtg attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca
    64981 ataccgtgat tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt
    65041 gccattagaa tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat
    65101 tgataaggtt tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt
    65161 ggtaaagaat cgacaacttc gttgacttga acgatcacga taccatcttt gaaagctgca
    65221 gcttcgatga ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta
    65281 tctgctttat ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca
    65341 taaagttcca aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg
    65401 gcgacacgca agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt
    65461 tcaaaaatat tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc
    65521 actttttctg catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg
    65581 tcgccttcta atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt
    65641 ttttcagttt ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct
    65701 tttttttcgc gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat
    65761 ttttgatata gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc
    65821 cctttatcta caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat
    65881 tcaatcgtca gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca
    65941 gggaaagcta ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt
    66001 ccatccgtat tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac
    66061 ggcttgaatt tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc
    66121 agcagcttcg ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct
    66181 tcgatactcc cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga
    66241 tggtcaggat caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact
    66301 actacgccca ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag
    66361 actagttccg ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca
    66421 ccaatcgaat gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa
    66481 taaaatactg cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct
    66541 tctttatcaa ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg
    66601 atatccgcta acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc
    66661 atgagcctcg cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat
    66721 aataaaaaat gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat
    66781 tatgaaattg cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca
    66841 agcagccaaa gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga
    66901 aaacgaatta ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta
    66961 tgcagaagaa ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa
    67021 attattgtct tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc
    67081 ctctgtttct attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt
    67141 aacgatcatg caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc
    67201 ggagatgaag cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat
    67261 tcctaagaaa catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac
    67321 ccccatcttg atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga
    67381 agcgaaaagt atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc
    67441 tattgtcaag caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa
    67501 aaatgatcct ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt
    67561 atcttctagc ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga
    67621 attttattta ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt
    67681 ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa
    67741 tttgagaagc aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca
    67801 acctcttctc tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat
    67861 ctgctagttc atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat
    67921 tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat
    67981 cgaccttcaa atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt
    68041 cttctggaac agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc
    68101 aataatcagg aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt
    68161 gatcgattag ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt
    68221 attcaaagat accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt
    68281 cgatattttt cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac
    68341 catctactat taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc
    68401 atttttcaag ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat
    68461 gattgaaaaa tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa
    68521 gggagtctga ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg
    68581 tcatgtttct ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc
    68641 tgaaatcata gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca
    68701 gctgcccgaa ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca
    68761 aaatttatta acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact
    68821 gctgaaaaat ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg
    68881 gctttcttat gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta
    68941 cgacttgcag atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat
    69001 tcgtactttt tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga
    69061 ttattcgatc catgaatccc attattttca atttttaaga aacctcagtc aaactgaact
    69121 agcggttgaa acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat
    69181 aaaagcgaat tgccgattag attcttctat ccataccaac agcacactct cctccctgta
    69241 tgactctgca ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct
    69301 ttcttttgct tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga
    69361 aaccgctcgc tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga
    69421 actagcttca gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc
    69481 tctcctcata ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
    69541 ccatcgtttt ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa
    69601 tttactgaac gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact
    69661 cttgtctcta ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta
    69721 ttttattttc caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta
    69781 cttaggaaaa cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt
    69841 tgaaaaaaca gatatcttga tttccaattt ccctctagat tctctagata tccctattgt
    69901 atacatttct tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa
    69961 aactttttta tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa
    70021 acagtttgtt catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg
    70081 ctccaatcaa taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga
    70141 ctggtagaaa aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg
    70201 ctttgttttg caaaagacct tggttcaatg acggcttgga caactctctg caattgatca
    70261 tgtaaacagc ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc
    70321 cttcaaactg catagccaat aaataggcag gaaccatgat cagtagggag acatagatga
    70381 tccgaaacaa atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca
    70441 tgatttctgg atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca
    70501 tattgaccca taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc
    70561 aaatcaagat caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca
    70621 aattatccaa aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat
    70681 ctcctaaaac catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa
    70741 ttcctacatc ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga
    70801 cgatttctcc atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc
    70861 gagcaaagac atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca
    70921 aatcgatccc ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt
    70981 ttgctgtgtc tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt
    71041 cctttacagc tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa
    71101 aagttaattg ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag
    71161 caaatcctaa aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt
    71221 cttgttctgc agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt
    71281 aaatcaccgc tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga
    71341 acggaatctt tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca
    71401 gcgcaacttc tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta
    71461 attcctgaca gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca
    71521 caactgtcat ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag
    71581 aacctaatgt ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat
    71641 gtactccaac ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca
    71701 ccaccaaagc aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg
    71761 taaaaataac taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc
    71821 ggttgatttt tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga
    71881 ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt
    71941 gtacaagcgt tcctgaaaaa gccatatttt tctggtcgcc gatcgattct tcttctgtca
    72001 aaacggcatg acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta
    72061 ataaatcatg tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga
    72121 caatgacatc tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata
    72181 gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc
    72241 tttcttgcca gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt
    72301 caatatattc actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca
    72361 gatcagtaaa atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt
    72421 cattatatcc ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg
    72481 atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc
    72541 ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat
    72601 ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct
    72661 attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg
    72721 aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa
    72781 caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac
    72841 atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa
    72901 aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa
    72961 taacattcct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa
    73021 ccaatggggc aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac
    73081 tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc
    73141 gccttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca
    73201 atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg
    73261 ccaaggcaga cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac
    73321 ggttaagccg atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac
    73381 cattccagca aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc
    73441 gatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa
    73501 aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc
    73561 aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac
    73621 atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc
    73681 aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata
    73741 gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc
    73801 ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc
    73861 tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa
    73921 tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa
    73981 gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta
    74041 ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta
    74101 gattgatcca tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata
    74161 aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa
    74221 atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga
    74281 catagacgat acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga
    74341 cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa
    74401 taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg
    74461 caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt
    74521 ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac
    74581 caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt
    74641 ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta
    74701 acgcggcaaa tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca
    74761 cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg
    74821 atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg
    74881 gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca
    74941 cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat
    75001 tcaaaagagt gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca
    75061 aaccaagcgg cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca
    75121 tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt
    75181 ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa
    75241 attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa
    75301 aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg
    75361 cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca
    75421 tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga
    75481 acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt
    75541 gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt
    75601 ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt
    75661 tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat
    75721 cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta
    75781 taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga
    75841 gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga
    75901 atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca
    75961 ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg
    76021 atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca
    76081 ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg
    76141 agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg
    76201 gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct
    76261 tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac
    76321 acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct
    76381 ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct
    76441 gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca
    76501 tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt
    76561 agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc
    76621 ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct
    76681 tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat
    76741 tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata
    76801 gcaaccatct gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt
    76861 tttgtttgag ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa
    76921 gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct
    76981 tcaacagata aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact
    77041 tccttgacag ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta
    77101 gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca
    77161 aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca
    77221 ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca
    77281 gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat
    77341 cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga
    77401 gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat
    77461 ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc attttcagct
    77521 ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt
    77581 tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc
    77641 aaaaatgaga aatttttttt tataacatca aatccacgta gatttttaca aacaaaaata
    77701 aataaagtcg gatttttctg ataattcaac gcaaaaaagc ga
//
LOCUS       DARDHD010000002        54696 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000002 DARDHD010000000
VERSION     DARDHD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.002"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDA06_000066"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDA06_000066"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG4227369.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDA06_000067"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDA06_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG4227370.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDA06_000068"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDA06_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG4227371.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDA06_000069"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDA06_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG4227372.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDA06_000070"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDA06_000070"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG4227373.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDA06_000071"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDA06_000071"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG4227374.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SDA06_000072"
     CDS             complement(8053..8523)
                     /locus_tag="SDA06_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG4227375.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SDA06_000073"
     CDS             complement(8569..8994)
                     /locus_tag="SDA06_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG4227376.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SDA06_000074"
     CDS             complement(9061..9330)
                     /locus_tag="SDA06_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG4227377.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SDA06_000075"
     CDS             complement(9341..10936)
                     /locus_tag="SDA06_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4227378.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SDA06_000076"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SDA06_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG4227379.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SDA06_000077"
     CDS             complement(14489..15049)
                     /locus_tag="SDA06_000077"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG4227380.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SDA06_000078"
     CDS             15402..16376
                     /locus_tag="SDA06_000078"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4227381.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SDA06_000079"
     CDS             16546..17946
                     /locus_tag="SDA06_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG4227382.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SDA06_000080"
     CDS             complement(18024..18746)
                     /locus_tag="SDA06_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4227383.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SDA06_000081"
     CDS             complement(18825..19331)
                     /locus_tag="SDA06_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4227384.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SDA06_000082"
     CDS             19454..21253
                     /locus_tag="SDA06_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG4227385.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SDA06_000083"
     CDS             complement(21321..22895)
                     /locus_tag="SDA06_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4227386.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SDA06_000084"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SDA06_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG4227387.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SDA06_000085"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SDA06_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG4227388.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SDA06_000086"
     CDS             complement(24672..25310)
                     /locus_tag="SDA06_000086"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG4227389.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SDA06_000087"
     CDS             complement(25311..25961)
                     /locus_tag="SDA06_000087"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG4227390.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SDA06_000088"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SDA06_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG4227391.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SDA06_000089"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SDA06_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG4227392.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SDA06_000090"
     CDS             complement(29104..29844)
                     /locus_tag="SDA06_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG4227393.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SDA06_000091"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SDA06_000091"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG4227394.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SDA06_000092"
     CDS             complement(31215..32153)
                     /locus_tag="SDA06_000092"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG4227395.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SDA06_000093"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SDA06_000093"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4227396.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SDA06_000094"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SDA06_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG4227397.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SDA06_000095"
     CDS             complement(35313..35618)
                     /locus_tag="SDA06_000095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4227398.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SDA06_000096"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SDA06_000096"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG4227399.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SDA06_000097"
     CDS             complement(36724..37008)
                     /locus_tag="SDA06_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227400.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SDA06_000098"
     CDS             complement(37026..37916)
                     /locus_tag="SDA06_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG4227401.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SDA06_000099"
     CDS             complement(37936..38154)
                     /locus_tag="SDA06_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG4227402.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SDA06_000100"
     CDS             complement(38124..39824)
                     /locus_tag="SDA06_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG4227403.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SDA06_000101"
     CDS             40091..40864
                     /locus_tag="SDA06_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227404.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SDA06_000102"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SDA06_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG4227405.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SDA06_000103"
     CDS             complement(41688..42659)
                     /locus_tag="SDA06_000103"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4227406.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SDA06_000104"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SDA06_000104"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG4227407.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SDA06_000105"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SDA06_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG4227408.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="SDA06_000106"
     CDS             complement(45614..46438)
                     /locus_tag="SDA06_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4227409.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="SDA06_000107"
     CDS             complement(46411..47091)
                     /locus_tag="SDA06_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4227410.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="SDA06_000108"
     CDS             complement(47114..48052)
                     /locus_tag="SDA06_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG4227411.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="SDA06_000109"
     CDS             complement(48297..49160)
                     /locus_tag="SDA06_000109"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG4227412.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SDA06_000110"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SDA06_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4227413.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="SDA06_000111"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="SDA06_000111"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG4227414.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SDA06_000112"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SDA06_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG4227415.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT        15115 a        11896 c         9812 g        17873 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctt
//
LOCUS       DARDHD010000003        52861 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000003 DARDHD010000000
VERSION     DARDHD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.003"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..634
                     /locus_tag="SDA06_000113"
     CDS             212..634
                     /locus_tag="SDA06_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4227416.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            631..861
                     /locus_tag="SDA06_000114"
     CDS             631..861
                     /locus_tag="SDA06_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227417.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1359..1559
                     /locus_tag="SDA06_000115"
     CDS             1359..1559
                     /locus_tag="SDA06_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4227418.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1586..2779
                     /locus_tag="SDA06_000116"
     CDS             1586..2779
                     /locus_tag="SDA06_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4227419.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(2847..4412)
                     /gene="guaA"
                     /locus_tag="SDA06_000117"
     CDS             complement(2847..4412)
                     /gene="guaA"
                     /locus_tag="SDA06_000117"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4227420.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4471..5394)
                     /locus_tag="SDA06_000118"
     CDS             complement(4471..5394)
                     /locus_tag="SDA06_000118"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG4227421.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5590..6195
                     /locus_tag="SDA06_000119"
     CDS             5590..6195
                     /locus_tag="SDA06_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4227422.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6503..7519
                     /locus_tag="SDA06_000120"
     CDS             6503..7519
                     /locus_tag="SDA06_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4227423.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7660..8961
                     /locus_tag="SDA06_000121"
     CDS             7660..8961
                     /locus_tag="SDA06_000121"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG4227424.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9007..9669
                     /gene="deoC"
                     /locus_tag="SDA06_000122"
     CDS             9007..9669
                     /gene="deoC"
                     /locus_tag="SDA06_000122"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG4227425.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9690..10088
                     /locus_tag="SDA06_000123"
     CDS             9690..10088
                     /locus_tag="SDA06_000123"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG4227426.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10154..11236
                     /locus_tag="SDA06_000124"
     CDS             10154..11236
                     /locus_tag="SDA06_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4227427.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11448..13010
                     /locus_tag="SDA06_000125"
     CDS             11448..13010
                     /locus_tag="SDA06_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227428.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13003..14136
                     /locus_tag="SDA06_000126"
     CDS             13003..14136
                     /locus_tag="SDA06_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227429.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14136..15092
                     /locus_tag="SDA06_000127"
     CDS             14136..15092
                     /locus_tag="SDA06_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227430.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15191..16357
                     /gene="deoB"
                     /locus_tag="SDA06_000128"
     CDS             15191..16357
                     /gene="deoB"
                     /locus_tag="SDA06_000128"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG4227431.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16381..17199
                     /locus_tag="SDA06_000129"
     CDS             16381..17199
                     /locus_tag="SDA06_000129"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4227432.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17216..17923
                     /gene="deoD"
                     /locus_tag="SDA06_000130"
     CDS             17216..17923
                     /gene="deoD"
                     /locus_tag="SDA06_000130"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4227433.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(18221..18907)
                     /gene="gpmA"
                     /locus_tag="SDA06_000131"
     CDS             complement(18221..18907)
                     /gene="gpmA"
                     /locus_tag="SDA06_000131"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4227434.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(19079..19660)
                     /locus_tag="SDA06_000132"
     CDS             complement(19079..19660)
                     /locus_tag="SDA06_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4227435.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(19793..20476)
                     /gene="rpiA"
                     /locus_tag="SDA06_000133"
     CDS             complement(19793..20476)
                     /gene="rpiA"
                     /locus_tag="SDA06_000133"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG4227436.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            20795..21208
                     /gene="rpsL"
                     /locus_tag="SDA06_000134"
     CDS             20795..21208
                     /gene="rpsL"
                     /locus_tag="SDA06_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG4227437.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            21292..21762
                     /gene="rpsG"
                     /locus_tag="SDA06_000135"
     CDS             21292..21762
                     /gene="rpsG"
                     /locus_tag="SDA06_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG4227438.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            21856..23943
                     /gene="fusA"
                     /locus_tag="SDA06_000136"
     CDS             21856..23943
                     /gene="fusA"
                     /locus_tag="SDA06_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4227439.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            24115..25302
                     /gene="tuf"
                     /locus_tag="SDA06_000137"
     CDS             24115..25302
                     /gene="tuf"
                     /locus_tag="SDA06_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4227440.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            25709..26017
                     /gene="rpsJ"
                     /locus_tag="SDA06_000138"
     CDS             25709..26017
                     /gene="rpsJ"
                     /locus_tag="SDA06_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG4227441.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            26048..26677
                     /gene="rplC"
                     /locus_tag="SDA06_000139"
     CDS             26048..26677
                     /gene="rplC"
                     /locus_tag="SDA06_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG4227442.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            26705..27328
                     /gene="rplD"
                     /locus_tag="SDA06_000140"
     CDS             26705..27328
                     /gene="rplD"
                     /locus_tag="SDA06_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG4227443.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            27328..27618
                     /gene="rplW"
                     /locus_tag="SDA06_000141"
     CDS             27328..27618
                     /gene="rplW"
                     /locus_tag="SDA06_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG4227444.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            27657..28490
                     /gene="rplB"
                     /locus_tag="SDA06_000142"
     CDS             27657..28490
                     /gene="rplB"
                     /locus_tag="SDA06_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG4227445.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            28533..28811
                     /gene="rpsS"
                     /locus_tag="SDA06_000143"
     CDS             28533..28811
                     /gene="rpsS"
                     /locus_tag="SDA06_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG4227446.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            28834..29181
                     /gene="rplV"
                     /locus_tag="SDA06_000144"
     CDS             28834..29181
                     /gene="rplV"
                     /locus_tag="SDA06_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG4227447.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            29195..29851
                     /gene="rpsC"
                     /locus_tag="SDA06_000145"
     CDS             29195..29851
                     /gene="rpsC"
                     /locus_tag="SDA06_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG4227448.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            29854..30288
                     /gene="rplP"
                     /locus_tag="SDA06_000146"
     CDS             29854..30288
                     /gene="rplP"
                     /locus_tag="SDA06_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG4227449.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            30278..30466
                     /gene="rpmC"
                     /locus_tag="SDA06_000147"
     CDS             30278..30466
                     /gene="rpmC"
                     /locus_tag="SDA06_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG4227450.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            30491..30757
                     /gene="rpsQ"
                     /locus_tag="SDA06_000148"
     CDS             30491..30757
                     /gene="rpsQ"
                     /locus_tag="SDA06_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG4227451.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            30815..31183
                     /gene="rplN"
                     /locus_tag="SDA06_000149"
     CDS             30815..31183
                     /gene="rplN"
                     /locus_tag="SDA06_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG4227452.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            31220..31528
                     /gene="rplX"
                     /locus_tag="SDA06_000150"
     CDS             31220..31528
                     /gene="rplX"
                     /locus_tag="SDA06_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG4227453.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            31556..32095
                     /gene="rplE"
                     /locus_tag="SDA06_000151"
     CDS             31556..32095
                     /gene="rplE"
                     /locus_tag="SDA06_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG4227454.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            32114..32299
                     /locus_tag="SDA06_000152"
     CDS             32114..32299
                     /locus_tag="SDA06_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG4227455.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            32335..32733
                     /gene="rpsH"
                     /locus_tag="SDA06_000153"
     CDS             32335..32733
                     /gene="rpsH"
                     /locus_tag="SDA06_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG4227456.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            32765..33301
                     /gene="rplF"
                     /locus_tag="SDA06_000154"
     CDS             32765..33301
                     /gene="rplF"
                     /locus_tag="SDA06_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG4227457.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            33462..33818
                     /gene="rplR"
                     /locus_tag="SDA06_000155"
     CDS             33462..33818
                     /gene="rplR"
                     /locus_tag="SDA06_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG4227458.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            33839..34339
                     /gene="rpsE"
                     /locus_tag="SDA06_000156"
     CDS             33839..34339
                     /gene="rpsE"
                     /locus_tag="SDA06_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG4227459.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            34354..34533
                     /gene="rpmD"
                     /locus_tag="SDA06_000157"
     CDS             34354..34533
                     /gene="rpmD"
                     /locus_tag="SDA06_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG4227460.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            34577..35017
                     /gene="rplO"
                     /locus_tag="SDA06_000158"
     CDS             34577..35017
                     /gene="rplO"
                     /locus_tag="SDA06_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG4227461.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35017..36312
                     /gene="secY"
                     /locus_tag="SDA06_000159"
     CDS             35017..36312
                     /gene="secY"
                     /locus_tag="SDA06_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG4227462.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            36374..37021
                     /locus_tag="SDA06_000160"
     CDS             36374..37021
                     /locus_tag="SDA06_000160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG4227463.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            37212..37430
                     /gene="infA"
                     /locus_tag="SDA06_000161"
     CDS             37212..37430
                     /gene="infA"
                     /locus_tag="SDA06_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG4227464.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            37463..37579
                     /gene="rpmJ"
                     /locus_tag="SDA06_000162"
     CDS             37463..37579
                     /gene="rpmJ"
                     /locus_tag="SDA06_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG4227465.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            37597..37962
                     /gene="rpsM"
                     /locus_tag="SDA06_000163"
     CDS             37597..37962
                     /gene="rpsM"
                     /locus_tag="SDA06_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG4227466.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            37990..38379
                     /gene="rpsK"
                     /locus_tag="SDA06_000164"
     CDS             37990..38379
                     /gene="rpsK"
                     /locus_tag="SDA06_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG4227467.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            38459..39397
                     /locus_tag="SDA06_000165"
     CDS             38459..39397
                     /locus_tag="SDA06_000165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG4227468.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            39425..39805
                     /gene="rplQ"
                     /locus_tag="SDA06_000166"
     CDS             39425..39805
                     /gene="rplQ"
                     /locus_tag="SDA06_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG4227469.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            40077..40916
                     /locus_tag="SDA06_000167"
     CDS             40077..40916
                     /locus_tag="SDA06_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4227470.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            40892..41761
                     /locus_tag="SDA06_000168"
     CDS             40892..41761
                     /locus_tag="SDA06_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4227471.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            41751..42548
                     /locus_tag="SDA06_000169"
     CDS             41751..42548
                     /locus_tag="SDA06_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4227472.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            42751..43497
                     /gene="truA"
                     /locus_tag="SDA06_000170"
     CDS             42751..43497
                     /gene="truA"
                     /locus_tag="SDA06_000170"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4227473.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(43830..46499)
                     /locus_tag="SDA06_000171"
     CDS             complement(43830..46499)
                     /locus_tag="SDA06_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG4227474.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(46737..46925)
                     /locus_tag="SDA06_000172"
     CDS             complement(46737..46925)
                     /locus_tag="SDA06_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG4227475.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            47195..47557
                     /locus_tag="SDA06_000173"
     CDS             47195..47557
                     /locus_tag="SDA06_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4227476.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            47609..49291
                     /locus_tag="SDA06_000174"
     CDS             47609..49291
                     /locus_tag="SDA06_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG4227477.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            49408..51444
                     /gene="recG"
                     /locus_tag="SDA06_000175"
     CDS             49408..51444
                     /gene="recG"
                     /locus_tag="SDA06_000175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG4227478.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            51493..52494
                     /gene="plsX"
                     /locus_tag="SDA06_000176"
     CDS             51493..52494
                     /gene="plsX"
                     /locus_tag="SDA06_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG4227479.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            52616..52858
                     /gene="acpP"
                     /locus_tag="SDA06_000177"
     CDS             52616..52858
                     /gene="acpP"
                     /locus_tag="SDA06_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4227480.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        17114 a         9673 c        11444 g        14630 t
ORIGIN      
        1 atgtggtttt gcagattaca tagatagaaa aatcaaagca tatgaacaga gaaaacaatc
       61 tgtttatgtg cttttttggt cacttaataa atatctttta ggatttctct tcatttatgc
      121 aacaaatccc ccgttcgtgt cggggtttag tatgaaggga gataaagaag cacatgccac
      181 tccctttcac gacagaaagg gggtgaaaag catgaaacca tcttcttttc aagaaaccat
      241 agaaaatcag tttgactata tctgcaaaca agttattgaa gatgaacgaa aagattactt
      301 caaacatcta gacagactat cagcaaaaga agtctcgttt tctgatatag ataactacct
      361 tgtcaactct ttttctactg ttgatacgta cgtcactgat ttccaactat ttaaattatt
      421 tggattaaga atcaatgttg aaagtgattt attaagtgaa gccctgcgtc atctttcaga
      481 gaaaaaacgg aacattattt tacttcatta ctttatggat atgagtgatg tggagattgc
      541 ggagttacta aatttgaatc gttcgactgt ttatcgtcat cgaataagtg gactagccat
      601 gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag cttatccaag ggttcccttc
      661 cccctcattg taaaggcgac agatggcgac gtggaagcaa ttaatcagat tgttaaacat
      721 tatcgagggt atacctcaaa acgttcatta cgtcgcatga cagacgaata tggaaattct
      781 catatggtta ttgatgaaac cctacgtggc agaatggaaa cgaaactcat ctctaagatt
      841 ttatcgttcg aaattaaata gtcccatttt ttcgtggaag cgtgtcgaat cgtatccacg
      901 cttcccgatt tgggaggttt gttattccat caaagcatat tgtgtgttca gtaggttttg
      961 ataggctaac aagccaaacg ttctttgaca accaaataaa ggcaatcgaa tacgtttcgg
     1021 taagaaaaga gctgatgaac taacacgcca tgacctacaa aattagtgag cgattcatgt
     1081 tagtaattcg aaagtaatct ttagcaggat tgctgccatg actttcttat cgtgataatg
     1141 atactcccgt atagccatag tccgagcgtt agaagcgtca caggcaatgg gtacggctac
     1201 atgagaacca tgcagaagtg aaattcctgt gagctttgct agagctgttc gattgctaag
     1261 atattcttct cttgtcagtg tattattata aaactatttg atataattat aggtgaatat
     1321 tggagttttc ctagcctata attgggaaag gagtaataat gataacggtc acaaaaaagg
     1381 atttagttga gctaggatat ggaacttctt tttcagcaga catcataaaa aaagcaaaag
     1441 aattgatgat tacaaaaggg catatctact atcaatctcg aaagctagat agagtccctc
     1501 gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa tgaaaacagg tctgagtagt
     1561 ttttgcacag taaggagtaa aaactatggt taaagaccca ataaaaaaag caaagaatgg
     1621 aacctattat tttcgtgcaa atctaggtta tgattctaaa gggaaaaaaa ttcaaaaata
     1681 tcgtagtgga ttcactacta aaaaagaagc aagagaagaa tattcgaaac tgctattgat
     1741 gaaaccagaa gaattaagtg agaataagga taaaatgacg tttaaacatt atatctttga
     1801 aattttcctt ccatggtata agacacaggt aaagctcaga acatatgaaa atcgcttacc
     1861 aactattaag aagcatttct cttattttga taaaatggct gtttccgata ttgagcctat
     1921 tgatgtgcaa aattggcaac taaaattatc aaagaagtgt aagtcttctt atgtaagagc
     1981 tgttcaagga ctattttcag ttgctatgga tcgagcaatt gtccttggga taaccaccac
     2041 aaatccatct aagattattg gcaatgtgaa aaagcaaaag tctaaaattg aattttggac
     2101 aaaagaagaa tttgaaaaag taatctctct aatttacaaa gaagattact atcaacactt
     2161 tttatttatc tctctgtggt ttttatttat gactggaatg cgaattggtg aagctactgc
     2221 cattcaatgg gaagatattg attttgactc aggagtacta accatcaaca aaacactcta
     2281 ttacaagaat caatcgaatt atagatttgt tgaaccaaaa acaaaggcga gcgttcgtca
     2341 tattgtatta gataaatgca cattaactta tttatccgaa tggaaggaag tacaacaaaa
     2401 tctaattcag acagactttg taatgagcta taacggtatt cctacccaaa aacatacact
     2461 atcatatgct attgaacgct atgctaagct ggctggtatc catcgaatca gaatccatgg
     2521 gttacggcat tctcatgctt ccttgttaat cagtatggga gaaaatccac ttatcattaa
     2581 agatcgtcta ggacacgaag atattgagac gactttagga acgtatggac atctttatcc
     2641 taacagtaat ttcgaggtag ctaacaaatt aaatggagcc atttccttta aagaagcaaa
     2701 acaaaattat gatacttcac caaagaatca atttactgta gactacttgc gaaacaaaga
     2761 aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga agagccagaa acccttggta
     2821 taagaggatt tctagctctc ctacaactat tcccactcca ctgttgctgg tggtttagaa
     2881 gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt
     2941 ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt
     3001 acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag
     3061 cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg
     3121 atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt
     3181 tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa
     3241 tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat
     3301 ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg
     3361 atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct
     3421 aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg
     3481 aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct
     3541 aagaagcgat ctcttgcatc gactttgatg atgttcaagc cgaattttcc gcctagactt
     3601 tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc
     3661 aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct
     3721 gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata
     3781 tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg
     3841 cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa
     3901 aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt
     3961 tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt
     4021 tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca
     4081 tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata
     4141 ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat
     4201 gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc
     4261 gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca
     4321 cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt
     4381 tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt
     4441 atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat
     4501 gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata
     4561 ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc
     4621 ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc
     4681 cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac
     4741 atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag
     4801 cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg
     4861 aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt
     4921 tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg
     4981 gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat
     5041 cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt
     5101 tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac
     5161 gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag
     5221 ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc
     5281 attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata
     5341 aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc
     5401 ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac
     5461 ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat
     5521 tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg
     5581 atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa
     5641 caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt
     5701 tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag
     5761 ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct
     5821 ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt
     5881 ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat
     5941 atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt
     6001 gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc
     6061 ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg
     6121 gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa
     6181 agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg
     6241 catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat
     6301 tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa
     6361 gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa
     6421 acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt
     6481 tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg
     6541 cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa
     6601 gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc
     6661 ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga
     6721 tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt
     6781 gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct
     6841 ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata
     6901 tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa
     6961 tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc
     7021 gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta
     7081 tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt
     7141 taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa
     7201 aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat
     7261 cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat
     7321 aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca
     7381 gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc
     7441 tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa
     7501 agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag
     7561 ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa
     7621 taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt
     7681 gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac
     7741 tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat
     7801 caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat
     7861 gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt
     7921 ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct
     7981 aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt
     8041 cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa
     8101 gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg
     8161 cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa
     8221 aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc
     8281 atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg
     8341 gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc
     8401 ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat
     8461 ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag
     8521 acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca
     8581 aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa
     8641 cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca
     8701 aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc
     8761 aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact
     8821 gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc
     8881 attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt
     8941 catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga
     9001 aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa
     9061 gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc
     9121 aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt
     9181 acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca
     9241 gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa
     9301 tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat
     9361 cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga
     9421 gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca
     9481 acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg
     9541 ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct
     9601 ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa
     9661 ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca
     9721 cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt
     9781 gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg
     9841 gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa
     9901 catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat
     9961 gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca
    10021 atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag
    10081 atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg
    10141 aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc
    10201 agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag
    10261 ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc
    10321 attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg
    10381 aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat
    10441 tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc
    10501 agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt
    10561 catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct
    10621 tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt
    10681 aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc
    10741 gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc
    10801 agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca
    10861 tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg
    10921 cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac
    10981 attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt
    11041 agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt
    11101 ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa
    11161 aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac
    11221 tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat
    11281 ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag
    11341 ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct
    11401 tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa
    11461 atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca
    11521 acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg
    11581 gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt
    11641 tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg
    11701 ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt
    11761 tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc
    11821 aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt
    11881 cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac
    11941 tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga
    12001 tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg
    12061 aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag
    12121 tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt
    12181 cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc
    12241 tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg
    12301 ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag
    12361 ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca
    12421 ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc
    12481 ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc
    12541 atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca
    12601 gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt
    12661 tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc
    12721 tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata
    12781 aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag
    12841 aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta
    12901 tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct
    12961 tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa
    13021 aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg
    13081 atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca
    13141 gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt
    13201 acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga
    13261 caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca
    13321 aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg
    13381 gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg
    13441 atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc
    13501 tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca
    13561 tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc
    13621 gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct
    13681 gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt
    13741 ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga
    13801 acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct
    13861 gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt
    13921 attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc
    13981 aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc
    14041 atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa
    14101 ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct
    14161 tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa
    14221 ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg
    14281 cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat
    14341 ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag
    14401 caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac
    14461 ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca
    14521 cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa
    14581 ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt
    14641 gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc
    14701 aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt
    14761 ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt
    14821 cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt
    14881 ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta
    14941 tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc
    15001 catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag
    15061 caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta
    15121 cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga
    15181 aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga
    15241 agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa
    15301 agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct
    15361 tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc
    15421 tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc
    15481 atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc
    15541 tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg
    15601 tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca
    15661 gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt
    15721 tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt
    15781 gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt
    15841 cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa
    15901 gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat
    15961 ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac
    16021 caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca
    16081 tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga
    16141 cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac
    16201 gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc
    16261 tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac
    16321 tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa
    16381 atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta
    16441 aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc
    16501 gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt
    16561 gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa
    16621 gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg
    16681 atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa
    16741 tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat
    16801 cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct
    16861 tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt
    16921 aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga
    16981 atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta
    17041 gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt
    17101 atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct
    17161 tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag
    17221 tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc
    17281 tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt
    17341 acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac
    17401 aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt
    17461 aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga
    17521 tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc
    17581 ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa
    17641 agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga
    17701 tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc
    17761 agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt
    17821 tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa
    17881 tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc
    17941 tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg
    18001 gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc
    18061 tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga
    18121 atattgtgac ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac
    18181 tgtgacaaat cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag
    18241 caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat
    18301 ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac
    18361 catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc
    18421 agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt
    18481 tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta
    18541 atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt
    18601 cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc
    18661 atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt
    18721 tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga
    18781 ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc
    18841 aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt
    18901 ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt
    18961 ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa
    19021 aacaagtaga gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt
    19081 agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa
    19141 ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac
    19201 gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt
    19261 tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg
    19321 ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat
    19381 caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc
    19441 gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt
    19501 cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct
    19561 catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa
    19621 cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt
    19681 gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt
    19741 tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac
    19801 gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca
    19861 tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc
    19921 tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg
    19981 ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa
    20041 tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa
    20101 gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga
    20161 ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga
    20221 tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg
    20281 gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac
    20341 gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac
    20401 cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta
    20461 attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca
    20521 ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt
    20581 tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt
    20641 gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata
    20701 aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa
    20761 ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg
    20821 taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa
    20881 cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg
    20941 tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg
    21001 tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga
    21061 acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca
    21121 tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa
    21181 atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct
    21241 actatataga agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt
    21301 aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta
    21361 actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc
    21421 tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa
    21481 caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca
    21541 aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg
    21601 gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa
    21661 atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa
    21721 atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc
    21781 ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga
    21841 ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc
    21901 atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt
    21961 aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa
    22021 gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac
    22081 cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca
    22141 ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa
    22201 actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac
    22261 aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg
    22321 caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt
    22381 atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa
    22441 gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt
    22501 gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc
    22561 actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca
    22621 gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt
    22681 gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat
    22741 gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt
    22801 atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa
    22861 tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta
    22921 cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct
    22981 gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt
    23041 attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca
    23101 aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact
    23161 ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt
    23221 cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt
    23281 gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt
    23341 gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat
    23401 gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa
    23461 tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc
    23521 tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca
    23581 aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct
    23641 aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta
    23701 ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc
    23761 gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct
    23821 acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac
    23881 gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa
    23941 taatttctta aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat
    24001 atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca
    24061 tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca
    24121 aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac
    24181 catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc
    24241 caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca
    24301 atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc
    24361 ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct
    24421 atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg
    24481 tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat
    24541 gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa
    24601 ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac
    24661 gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact
    24721 ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact
    24781 ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa
    24841 gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg
    24901 ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt
    24961 gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct
    25021 catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact
    25081 ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt
    25141 gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt
    25201 gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga
    25261 cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag
    25321 taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg
    25381 agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac
    25441 acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac
    25501 aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt
    25561 agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac
    25621 acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact
    25681 tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa
    25741 agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag
    25801 aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat
    25861 catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa
    25921 acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga
    25981 cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg
    26041 tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga
    26101 atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt
    26161 gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga
    26221 agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg
    26281 cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac
    26341 agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg
    26401 attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg
    26461 ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa
    26521 acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg
    26581 tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc
    26641 tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta
    26701 aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact
    26761 taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat
    26821 gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg
    26881 cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg
    26941 ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta
    27001 caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc
    27061 tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt
    27121 caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg
    27181 taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga
    27241 taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct
    27301 tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg
    27361 atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact
    27421 cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa
    27481 aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat
    27541 acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta
    27601 ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg
    27661 cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg
    27721 ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg
    27781 gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat
    27841 atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg
    27901 aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag
    27961 cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag
    28021 cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc
    28081 acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg
    28141 ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc
    28201 gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat
    28261 taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg
    28321 gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg
    28381 gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca
    28441 aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac
    28501 catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt
    28561 ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag
    28621 ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg
    28681 cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca
    28741 aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa
    28801 cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa
    28861 gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc
    28921 agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct
    28981 ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta
    29041 gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt
    29101 ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg
    29161 gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg
    29221 aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc
    29281 tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc
    29341 tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac
    29401 agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga
    29461 attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga
    29521 tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt
    29581 ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa
    29641 aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga
    29701 aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga
    29761 tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac
    29821 taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac
    29881 cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc
    29941 ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct
    30001 gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc
    30061 cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca
    30121 cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt
    30181 cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact
    30241 aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga
    30301 attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct
    30361 tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa
    30421 atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc
    30481 cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc
    30541 agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta
    30601 tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa
    30661 agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg
    30721 tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag
    30781 attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa
    30841 agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg
    30901 tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg
    30961 tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc
    31021 tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga
    31081 tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa
    31141 caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca
    31201 aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg
    31261 aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat
    31321 tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg
    31381 tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac
    31441 tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa
    31501 aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa
    31561 ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta
    31621 tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga
    31681 tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg
    31741 tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg
    31801 aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa
    31861 attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt
    31921 tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga
    31981 ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc
    32041 tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa
    32101 ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt
    32161 ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa
    32221 gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt
    32281 gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc
    32341 atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat
    32401 gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt
    32461 gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt
    32521 ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa
    32581 ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt
    32641 atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat
    32701 atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct
    32761 ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca
    32821 agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc
    32881 agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa
    32941 agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt
    33001 cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca
    33061 aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc
    33121 tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga
    33181 agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg
    33241 taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata
    33301 atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta
    33361 ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact
    33421 gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata
    33481 agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg
    33541 agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg
    33601 acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa
    33661 caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag
    33721 gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt
    33781 tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat
    33841 ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt
    33901 aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga
    33961 caaaaacgga cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg
    34021 taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat
    34081 cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga
    34141 aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc
    34201 agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt
    34261 tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt
    34321 tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa
    34381 cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca
    34441 aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact
    34501 gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta
    34561 aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt
    34621 acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca
    34681 aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac
    34741 accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc
    34801 agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc
    34861 tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg
    34921 ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc
    34981 aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct
    35041 tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt
    35101 cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct
    35161 tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc
    35221 attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa
    35281 atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa
    35341 ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc
    35401 actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact
    35461 tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa
    35521 cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt
    35581 tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct
    35641 tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc
    35701 gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa
    35761 cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt
    35821 attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg
    35881 agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt taactataat
    35941 acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc
    36001 tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata
    36061 ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta
    36121 agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg
    36181 gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt
    36241 gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga
    36301 ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt
    36361 aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca
    36421 agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg
    36481 cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg
    36541 tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga
    36601 tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct
    36661 ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg
    36721 tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac
    36781 ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca
    36841 tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa
    36901 catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat
    36961 tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata
    37021 gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta
    37081 tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt
    37141 tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg
    37201 aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt
    37261 tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt
    37321 caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat
    37381 tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg
    37441 taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca
    37501 ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca
    37561 taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat
    37621 attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact
    37681 actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta
    37741 acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat
    37801 cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga
    37861 atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact
    37921 cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt
    37981 taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa
    38041 gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat cactgatact
    38101 cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa
    38161 tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac
    38221 ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt
    38281 cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct
    38341 cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt
    38401 tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat
    38461 gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt
    38521 catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat
    38581 tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca
    38641 tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa
    38701 aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg
    38761 acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa
    38821 agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa
    38881 acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt
    38941 tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac
    39001 acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc
    39061 aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat
    39121 ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac
    39181 acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta
    39241 caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc
    39301 tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt
    39361 gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac
    39421 caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga
    39481 tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga
    39541 agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg
    39601 tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga
    39661 catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga
    39721 acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc
    39781 aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat agatgattct
    39841 tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa
    39901 gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac
    39961 ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa
    40021 attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg
    40081 agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg
    40141 cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca
    40201 atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag
    40261 ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga
    40321 tggttggaat gatttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg
    40381 acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga
    40441 aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat
    40501 caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca
    40561 ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta
    40621 cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg
    40681 atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag
    40741 gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac
    40801 catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat
    40861 atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt
    40921 cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat
    40981 caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat caaccttact
    41041 tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga
    41101 tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt
    41161 ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg
    41221 tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga
    41281 acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca
    41341 aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga
    41401 tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg
    41461 tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc
    41521 caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc
    41581 gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc
    41641 caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac
    41701 agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata
    41761 aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag
    41821 ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa
    41881 cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga
    41941 aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc
    42001 aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg
    42061 aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt
    42121 cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc
    42181 ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg
    42241 ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg
    42301 ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt
    42361 tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg
    42421 aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata
    42481 atagagacac gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa
    42541 gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa
    42601 ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc
    42661 tctttctttt atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga
    42721 taacctttta ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac
    42781 gacggaacaa attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa
    42841 gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga
    42901 aggacagatg ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag
    42961 cgtccgttag aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta
    43021 agacaagtag aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg
    43081 tatcaatttc gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt
    43141 tattttcctt acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg
    43201 actcacgatt tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg
    43261 atctacgagg ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg
    43321 ggaaatggct tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc
    43381 aatggccgat tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca
    43441 gcaggaccta cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag
    43501 gttatgatag aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca
    43561 aaattttgca aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagaa
    43621 agaggtcgtg agcctgtcat ttaactctga gtcacaaaga gcaatttcaa aaaacagctt
    43681 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    43741 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttggtgc
    43801 ggacagtctg aacaggttcc atcgtttctt tattttctac gtcttcttct gtgtgtgttt
    43861 cgtggttgtt cttctattgc tggatcaatc gtgccataaa aggggcgatt gcggtcaagg
    43921 taccagacat aaagaataaa caatgcaata cataggacaa aaaggattgc acctatcgta
    43981 aatccttcag gcaagtaact gaaagtgaca gtatgtttac ctgctggaac ttttatcatc
    44041 aagaatgcgt ctttgaaatt gctgaccttg actggtttcc catcaatttt agctttccat
    44101 cctttgtcat aaggaatagt tgtcaccaac atggaatctt cagcggtatt tacagtccca
    44161 gaagcttttc tgccagtggc ttccatttcg acaccttttt ctttgatctg attcattgcg
    44221 gcttgatagg ctacaacatc tagaccaacg actttaggct ccatgaaact tatttctttc
    44281 gtcccataaa aacttgcagt aaatgtcact gttgtctctt gaggataata gccaagatca
    44341 tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg taccattgac agtgatcgtc
    44401 gcactagaac tttctaattg cgcaaaattg gttgggaaca aacttaagta agcttgtgta
    44461 tttgcaggca catggacttc ccaagtgagc tcttttggct ggttatctgc tgtttctgag
    44521 taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca actgattctt caaagtaggt
    44581 gtatagaatg taaaatactg ttcatttaga ccagacaatg tattgatcaa gtttgtctga
    44641 ctggtcaaat tatctgtcac tggctgctca acatcattaa cggacaacgg tgcagtaaat
    44701 gctagtggca atgcattgac attctgatac agtgtatagt cgccgctttg accagcttgg
    44761 cggaagccat atttactgaa cgtgctttcg tttttagaaa tgttatattt gattcctgta
    44821 aacccatcca tcagcaaagt attattgtta taacggatat tcaaatttgt ccctcttgaa
    44881 cggaaaccta atttatccag gtaagaggaa gaattacgat ttcttataga agaaaacaaa
    44941 ctgataccac tgtaaccata gttgatcgaa tcgtttgcag atactggatc cagattttcc
    45001 aagcggtaaa acgagtcgga atcttttttt gtttcattta caagagcttt caagtcagga
    45061 taaggatcag tatataaact gcgggaagca taattccaat catcaagaat gccatggatc
    45121 atactgttcg tattgaccag tgcttcagca gacatgatca ataataacaa tatactcaga
    45181 tgattgaatg aaagtttttt atgctgataa aatcccacag caaaagcata aatccctaag
    45241 aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg
    45301 atgccccatg ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa caacagccca
    45361 ccatcgtctt gttctatctt ttcccatcct tttcctgcaa gcaggagcac taagaaagag
    45421 aacagatagg cataacggaa taaaaacata ttcggtgcat gcataccatg ccagaacaaa
    45481 ttcaatggaa caagataaaa acttgcgata agtatggcga aaagactgcc gtacaaaaat
    45541 ttatttttca acggtacttt ccgactggca aaataaaaca gacagaggac taatggtagc
    45601 agacctgcgt aaacgaacgg aatcgatccg tattttgtcg tgtcataaac accaatcata
    45661 tttttcatga ttagatccag ataagaagtc gcttctgttt tcaacgtagt gatctgactc
    45721 agtgcctcgc catttgaccg aagatccagc actgctggca atacaatgat cattgaagcg
    45781 ccgcctgcca gtaaagaagt gataccataa ggaacaatcg tttttttata ttctgaccag
    45841 tttgtcaata gacgggcgat aaaatataag aaagaaaata cgccaatcat aaaacccata
    45901 taaaaactgg ataaaaacaa gagtaaataa ctgataaata aaagtttcgg ccgtttaaga
    45961 tccataacac gatgaatccc caaaatcact aatggaagat aaacaaatgc atccagccac
    46021 atgatgatct ccgaatgtgc agtgataaat gagatcaatg cgtaactgac gctaagtgca
    46081 acatgccccc agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc caatccggca
    46141 cagccgacct taagcaaagt cagaacatac agtgcatctg gcatcatctg gtttggaaaa
    46201 aagaacacca gtggcgtgaa gatcccgcct agatagtaag aaattaaaga taaataattc
    46261 aatcctagag aagcattcca tgtataaaaa atactttgct ttccaagaag aacatttcgg
    46321 aagctggcat gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat acttctgctg
    46381 cttccaggat agattccgat acttagataa acaagtgcta aaatcaaaaa cggaatcagg
    46441 aaactagctg ctgcgtagcg tccgttttct ctaaagaatg actttaaatt ttttttcata
    46501 tttattttcc accaaatttc tatgaagttg tattcagttt atcatatcct tgtttttctc
    46561 tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt agaataaata acttcataat
    46621 ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa
    46681 aaatttttat taaataaaaa accgttcact atctgaacgg tccaagtatc attcaattaa
    46741 acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt
    46801 ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc
    46861 atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt
    46921 gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca
    46981 gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata caattgacaa
    47041 ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag
    47101 tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa
    47161 atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc
    47221 tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga
    47281 tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat
    47341 tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc
    47401 tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt
    47461 ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa
    47521 tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca
    47581 gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt
    47641 ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc
    47701 attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac
    47761 tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat
    47821 gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt
    47881 gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt ctgatcttgc
    47941 tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg
    48001 aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac
    48061 tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa
    48121 aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg
    48181 actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga
    48241 aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag
    48301 tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt
    48361 aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta
    48421 tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca
    48481 cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt
    48541 caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga
    48601 agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg tagcgatcgc
    48661 tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg
    48721 tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc
    48781 agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc
    48841 agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac
    48901 agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga
    48961 aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga
    49021 cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc
    49081 taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac
    49141 agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc
    49201 tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc
    49261 tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca
    49321 gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt
    49381 ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg
    49441 gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa
    49501 gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa tctaaacgag
    49561 atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat
    49621 cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc
    49681 aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc
    49741 gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa gattttaggt
    49801 tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg
    49861 acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat
    49921 ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt
    49981 atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa
    50041 gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg
    50101 aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca aaagctacct
    50161 tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt
    50221 ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc
    50281 gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca
    50341 gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta
    50401 accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa
    50461 ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca agatggagtc
    50521 gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag
    50581 cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg
    50641 attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa
    50701 cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt
    50761 gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg
    50821 ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt
    50881 cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag
    50941 gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact
    51001 actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg
    51061 gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg cggaagtgat
    51121 gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa
    51181 atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt
    51241 cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg
    51301 gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcaaata
    51361 aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat
    51421 caggataatc gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa
    51481 ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca
    51541 attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac
    51601 ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat
    51661 acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact
    51721 gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca
    51781 gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat
    51841 atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc
    51901 gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt
    51961 attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc
    52021 ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc
    52081 cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat
    52141 ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt
    52201 gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc
    52261 aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg
    52321 gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact
    52381 catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt
    52441 gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg
    52501 gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta
    52561 aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac
    52621 tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac
    52681 cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat
    52741 tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc
    52801 tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt
    52861 t
//
LOCUS       DARDHD010000004        51554 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000004 DARDHD010000000
VERSION     DARDHD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.004"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..851
                     /locus_tag="SDA06_000178"
     CDS             132..851
                     /locus_tag="SDA06_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG4227481.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            complement(1150..3048)
                     /locus_tag="SDA06_000179"
     CDS             complement(1150..3048)
                     /locus_tag="SDA06_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG4227482.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(3211..4473)
                     /locus_tag="SDA06_000180"
     CDS             complement(3211..4473)
                     /locus_tag="SDA06_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227483.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(4466..5365)
                     /locus_tag="SDA06_000181"
     CDS             complement(4466..5365)
                     /locus_tag="SDA06_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227484.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(5510..7288)
                     /gene="uvrC"
                     /locus_tag="SDA06_000182"
     CDS             complement(5510..7288)
                     /gene="uvrC"
                     /locus_tag="SDA06_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG4227485.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(7470..7784)
                     /gene="trxA"
                     /locus_tag="SDA06_000183"
     CDS             complement(7470..7784)
                     /gene="trxA"
                     /locus_tag="SDA06_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG4227486.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(7871..10231)
                     /locus_tag="SDA06_000184"
     CDS             complement(7871..10231)
                     /locus_tag="SDA06_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4227487.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(10346..10893)
                     /locus_tag="SDA06_000185"
                     /pseudo
     CDS             complement(10346..10893)
                     /locus_tag="SDA06_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(10951..11388)
                     /gene="zapA"
                     /locus_tag="SDA06_000186"
     CDS             complement(10951..11388)
                     /gene="zapA"
                     /locus_tag="SDA06_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG4227488.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            11590..12507
                     /locus_tag="SDA06_000187"
     CDS             11590..12507
                     /locus_tag="SDA06_000187"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG4227489.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(12763..13674)
                     /locus_tag="SDA06_000188"
     CDS             complement(12763..13674)
                     /locus_tag="SDA06_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4227490.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(14077..14655)
                     /locus_tag="SDA06_000189"
     CDS             complement(14077..14655)
                     /locus_tag="SDA06_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4227491.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(14675..15343)
                     /locus_tag="SDA06_000190"
     CDS             complement(14675..15343)
                     /locus_tag="SDA06_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227492.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(15358..16428)
                     /locus_tag="SDA06_000191"
     CDS             complement(15358..16428)
                     /locus_tag="SDA06_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227493.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(16599..17963)
                     /locus_tag="SDA06_000192"
     CDS             complement(16599..17963)
                     /locus_tag="SDA06_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4227494.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(17960..19519)
                     /locus_tag="SDA06_000193"
     CDS             complement(17960..19519)
                     /locus_tag="SDA06_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227495.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(19575..22055)
                     /locus_tag="SDA06_000194"
     CDS             complement(19575..22055)
                     /locus_tag="SDA06_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227496.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(22039..22374)
                     /locus_tag="SDA06_000195"
     CDS             complement(22039..22374)
                     /locus_tag="SDA06_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227497.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(22388..23422)
                     /locus_tag="SDA06_000196"
     CDS             complement(22388..23422)
                     /locus_tag="SDA06_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4227498.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(23512..24192)
                     /locus_tag="SDA06_000197"
     CDS             complement(23512..24192)
                     /locus_tag="SDA06_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4227499.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(24476..25579)
                     /locus_tag="SDA06_000198"
     CDS             complement(24476..25579)
                     /locus_tag="SDA06_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4227500.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(26208..26678)
                     /locus_tag="SDA06_000199"
     CDS             complement(26208..26678)
                     /locus_tag="SDA06_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG4227501.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            27008..28648
                     /locus_tag="SDA06_000200"
     CDS             27008..28648
                     /locus_tag="SDA06_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG4227502.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            28802..30124
                     /locus_tag="SDA06_000201"
     CDS             28802..30124
                     /locus_tag="SDA06_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG4227503.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(30192..30626)
                     /locus_tag="SDA06_000202"
     CDS             complement(30192..30626)
                     /locus_tag="SDA06_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4227504.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(30691..31528)
                     /locus_tag="SDA06_000203"
                     /pseudo
     CDS             complement(30691..31528)
                     /locus_tag="SDA06_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(32270..32896)
                     /locus_tag="SDA06_000204"
     CDS             complement(32270..32896)
                     /locus_tag="SDA06_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227505.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(33191..33526)
                     /locus_tag="SDA06_000205"
     CDS             complement(33191..33526)
                     /locus_tag="SDA06_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4227506.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(33513..33797)
                     /locus_tag="SDA06_000206"
     CDS             complement(33513..33797)
                     /locus_tag="SDA06_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4227507.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(33853..35376)
                     /locus_tag="SDA06_000207"
     CDS             complement(33853..35376)
                     /locus_tag="SDA06_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4227508.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(35369..36544)
                     /locus_tag="SDA06_000208"
     CDS             complement(35369..36544)
                     /locus_tag="SDA06_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4227509.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(36548..36733)
                     /locus_tag="SDA06_000209"
     CDS             complement(36548..36733)
                     /locus_tag="SDA06_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227510.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(36699..38393)
                     /locus_tag="SDA06_000210"
     CDS             complement(36699..38393)
                     /locus_tag="SDA06_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4227511.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(38390..38863)
                     /locus_tag="SDA06_000211"
     CDS             complement(38390..38863)
                     /locus_tag="SDA06_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG4227512.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(38932..39087)
                     /locus_tag="SDA06_000212"
     CDS             complement(38932..39087)
                     /locus_tag="SDA06_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG4227513.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(39221..39601)
                     /locus_tag="SDA06_000213"
     CDS             complement(39221..39601)
                     /locus_tag="SDA06_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4227514.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(39605..39820)
                     /locus_tag="SDA06_000214"
     CDS             complement(39605..39820)
                     /locus_tag="SDA06_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227515.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(39823..40230)
                     /locus_tag="SDA06_000215"
     CDS             complement(39823..40230)
                     /locus_tag="SDA06_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG4227516.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(40496..41971)
                     /locus_tag="SDA06_000216"
     CDS             complement(40496..41971)
                     /locus_tag="SDA06_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG4227517.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(41961..42809)
                     /locus_tag="SDA06_000217"
     CDS             complement(41961..42809)
                     /locus_tag="SDA06_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG4227518.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(42846..43208)
                     /locus_tag="SDA06_000218"
     CDS             complement(42846..43208)
                     /locus_tag="SDA06_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227519.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(43209..43367)
                     /locus_tag="SDA06_000219"
     CDS             complement(43209..43367)
                     /locus_tag="SDA06_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227520.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(43508..44132)
                     /locus_tag="SDA06_000220"
                     /pseudo
     CDS             complement(43508..44132)
                     /locus_tag="SDA06_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(44129..44440)
                     /locus_tag="SDA06_000221"
     CDS             complement(44129..44440)
                     /locus_tag="SDA06_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227521.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(44484..44777)
                     /locus_tag="SDA06_000222"
     CDS             complement(44484..44777)
                     /locus_tag="SDA06_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227522.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            44971..45618
                     /locus_tag="SDA06_000223"
     CDS             44971..45618
                     /locus_tag="SDA06_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4227523.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            45679..46824
                     /locus_tag="SDA06_000224"
     CDS             45679..46824
                     /locus_tag="SDA06_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4227524.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(46905..47210)
                     /locus_tag="SDA06_000225"
     CDS             complement(46905..47210)
                     /locus_tag="SDA06_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG4227525.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(47235..47666)
                     /gene="ruvX"
                     /locus_tag="SDA06_000226"
     CDS             complement(47235..47666)
                     /gene="ruvX"
                     /locus_tag="SDA06_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG4227526.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(47663..47929)
                     /locus_tag="SDA06_000227"
     CDS             complement(47663..47929)
                     /locus_tag="SDA06_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG4227527.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(48184..48909)
                     /locus_tag="SDA06_000228"
     CDS             complement(48184..48909)
                     /locus_tag="SDA06_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4227528.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(49270..49340)
                     /locus_tag="SDA06_000229"
     tRNA            complement(49270..49340)
                     /locus_tag="SDA06_000229"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49306..49308),aa:Gly,seq:ccc)
     gene            49457..50209
                     /locus_tag="SDA06_000230"
     CDS             49457..50209
                     /locus_tag="SDA06_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4227529.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            50225..50704
                     /locus_tag="SDA06_000231"
     CDS             50225..50704
                     /locus_tag="SDA06_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4227530.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(50955..>51554)
                     /locus_tag="SDA06_000232"
     CDS             complement(50955..>51554)
                     /locus_tag="SDA06_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG4227531.1"
                     /translation="ALGGEIKLTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLD
                     QENFLFIRSLPISMNQYLKEKFRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGS
                     LFGCYLLSLRYFARDYRLLLLNWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYG
                     MLALFYPFWLVNIPVIGLVLILSALWIVYYQRTFWKKFE"
BASE COUNT        14885 a        10623 c         8772 g        17274 t
ORIGIN      
        1 atgaatttca cacgaaaatt ctctttacag atagtaacac agcaagaaca gataagtata
       61 ggggcaagtc ttacatagtt taaatttttt taaatccttg ttacaattag tctactttag
      121 ggggaataaa aatgaaacca atcatcggca ttgccgggaa tcaactgatt cgagcgacag
      181 atactttcca aggaaatcaa gtaagctata caccgcaggg atttgtggat gcagttttag
      241 atgctcaagg attacctctt attctgccag tcatgtctcc agattcagcg cctcaactaa
      301 ttgagaaaat cgacaaactc attcttgcag gcggtcagga tgttagtccg caactttaca
      361 tggaagaccc tcatccaaaa ctgacggaaa caaatattca acgagaccaa ttcgaacagg
      421 cattgatctt ggaagcattg aaacaaagaa aaccgatttt tgcagtctgc cgaggacttc
      481 aactattgaa tgtcgtgttg gagggaacac tttatcaaga tctttcatta tatccaaaat
      541 ggtccgtaaa acacgaacaa cagcccactg ccccgcagtt tgcaactcac gaagtcaaaa
      601 tcgtttcgga cagtttatta agtgaccttc tccctgattc atactttgtg aattcctatc
      661 atcatcaggc agtcaaagat ctttctcctt tgttaaaagc catcgccttt tccaacgatg
      721 gactcattga agcagtccaa agtaaagata atatgcacaa aatccttggt gtccaatggc
      781 atccggaact ttctcatcgt gtagattcat ctgaacagcg tttattcgat ttctttgtga
      841 atgaatttta atcgtttttc tgattaggtc gtgagcctgc cgttcaactc tgagtcacaa
      901 ggagcaattt caaaaaacag cttctcatgt ttgttggata ttgtgacttg tgaccgagga
      961 gttggcagga gaacaccgtt taaggaagag gtcgtgaaat cagcgccctg acctgagtat
     1021 tagagaaata aacagaaaaa tagcttctaa tattttaccg ttttttgagc taattccgaa
     1081 ggagactgcg acacgccttt tgttgcagtc gctttttagt ctattgagca atgttggcta
     1141 gcatttttct caatcgtcct ttcgataatc caacagcctt tggagttgag taatctgttg
     1201 ctgtaattcc attccttttg tcgtattccg attaagtttg cgtgggagct gctgctcgat
     1261 cacttttttt acattcgttt gatcagattt ttttgcaaat tgttgttcaa taggcaagaa
     1321 cggataatgt gtcacgattt ggaaaccgta agaattgcat agtaatgagt agccagcaat
     1381 cccggttgtt ttttgataag ctttggatag acctccgtca ataacaaatg cttttccgtt
     1441 tgctttgatc ggtgactctc ctttttttac ttttactggc gtgtgtccat tgatgattgc
     1501 tgaaccttct tcttttgcat cgaattctgc caaaattttt tcactgaacc actcttcatc
     1561 tcgcaagtga taatagggat tgcttttttc gacgtgcgta tgaggctctg tgagaaaata
     1621 gcgctcaaaa gttgccattt tactcttgcc gaataaaggc gaaaatggcc cgctccacgc
     1681 ataccaaatc aaatcggtcg ccaaatcttc atttgcagtc aaatcttttg cgctttcagt
     1741 tatctgtttt tcaaaaaatc ccagcagttc ttttccgcga taagattggt ctaattcaaa
     1801 tgctaagagc tgcccctttt catctacagg tatacagcca tggaacagta catgctgatt
     1861 gttcgtgaga tacattcccc ctttttcgat caaaaacgtc atgtgttttt gcattcttaa
     1921 cgagtgctgg aatgaataca tcaaactatc gatcactttt tgttcctctt ccaagaattt
     1981 tttaggatca tctggctgga tcgtttgaaa acaagtccct ttcaactcat agagctgacc
     2041 ttctaaaacg atcgttgatt cctcatagtt gattttttcc aacaatttac gatcgtccat
     2101 tttaaattca ggtcgtcgat tcaacaactg atgttctgat ttgaattgga ggatagaaag
     2161 tgcttgatgg atcttttcta attcttcttg ctctctttca gatagatctt tcctgttttt
     2221 aggggtaaat actggattct tctgataggt ttgatgggca aaagaaaata atggacgtaa
     2281 atttaatcca tattctttct ctaattcaaa taaatagttg tagcgtgcgg caatccgtaa
     2341 aagcgtcaat aaacatgcct cagagccgca atacgcacca atccacaaaa tatcatgatt
     2401 cccccactgg atatctacag aggaaaaatc catgattcga tccattactt tggctgcttg
     2461 agttcctcga tcaaaaatat cacccacgat atgcaaatga tcgatgacag atttttggat
     2521 cgtctgcgca agttttgtta aaaatttttc ttcttccttc atcaagacaa gttgttcaat
     2581 gatttttttt acatagtctt ttttcccgtt gatacgatca tcgatataca agagttcctc
     2641 aatgatatag ctatattctt tcggcaatcc ttttcgaact tttgatcgag tgtatttgac
     2701 tgtcgtaaac cgtaaaagat cgatcaaacg atggatcagc tgggatttat cttgtttttt
     2761 gtaccaatca gtcgtcaaca catattctgg ataagccgta aaaatagtga attggctgat
     2821 ctccgtttcc gtcatttgtt cagagaaaag gtctttgatc ttttctttaa gatttcctga
     2881 accgatacgc agaatatgat caaaagcagg aaattcgcca tgtatatcgc tcaaaaataa
     2941 ctctgttcct tttggaagct gcaaaattgc ttcgagattg atgatttccg tgatgacatc
     3001 ttccgttgta gggaacttct ctgaaagtaa atctaaatag cggtccatta aaacgcctcc
     3061 aaaaattggt atactcaaca ttataaagtt gttatgctct attttcaatg aatatatata
     3121 tccaatataa taaaaaaata gaaataggtc tatacaaaaa agaagctggg aacaataaat
     3181 gtccagcttc cttctatatt tttctatatt ttatgctttt tttcgttcat ttctcataat
     3241 tgaaatcgat tgtttcaatg aactccataa tccgccttcg ctatacacga gtacattcga
     3301 tttgtacatg tttgcagaca agatcatcaa taagaatgtt acggctacaa gtaccaacaa
     3361 ggaaatcaat gctccaccca taccgactgt ctcatttgct aagcgaaccg gcataatata
     3421 tgaagataag aatggaatat acgaagtgat ccgaataacg atattgtttg gatctgaagc
     3481 tcctagtatc aatccaagca tatatccgcc tagagataga taagtcactg gcaaaattgc
     3541 ttttgctgta tcttctgctt tgttgaccaa agagccacat aaagctgcta aaacagaaaa
     3601 aatcaaaatg ccaaagatca tgaatagaag agaataccac aagaatggtc caatcaagtt
     3661 atccaatgag ataccgtcta atatattttt gaccatttcc atatctttga attgactgta
     3721 tcctatacca aaaataatac cgtataatgc catttgcgtt aatgcaacaa gtaaaacacc
     3781 tgttagtttt ccgtaaaaat gcttttgtgc agtcgtactg gacaggatca cttccatgat
     3841 ccgcgtgcct ttttcagaag cgatctcttg tgcaatgatc tgcgcatacg tcaaaataat
     3901 aatgaacaag acaatcgttg ctacataact tacgacatat tggactgtcg agttatcttc
     3961 tccaactgtc atttttcctt ttgaatcaaa attgactttt tgtcgagtca aacttgcttg
     4021 ttggctcaag ctagctactt cttctggtga aatgcctaat tgacttgctc gaagcatcga
     4081 ttgcaagcca gtcagttgtt gttgtatcag tagttgtgta ctttgtccta atgaattttc
     4141 gctataaagc atcccattta tcttatcatt ctcagttgag actactaaat acgcatcgat
     4201 tttttcttcc gaaagctctt tttgtgcttt ttcttcagaa tcaactgctt tgaactcaaa
     4261 atcatctgtt ttgacttggc tcatttgctg actgatttgc tcattatcag ccaatacgcc
     4321 gattttattg acatcactat tttgttgagc aaagtttcct gctacataaa tgatacccag
     4381 catgataaaa gggactaata tcatgatcaa gaaagaaatc gacttaacat tttttaaata
     4441 gacatctttg gtaataatcc aaaacttacg catcgatttc acctgctttc aatttgaaaa
     4501 tctcttccaa agttggcggt tgctggttaa acataggaat gtagccgaac tgagtcgcac
     4561 gagtaaagat ttctttccca gcttcaggat cttctaaggt aatctctaac gtattgtctt
     4621 catgaaggat cacttttttc acgccttcga tttcttctac ttcctgttgg cttaaaccag
     4681 attccaagaa taacttcgta cggccaaatg attcacgaat ctcatgtact tttccattca
     4741 aaaccatttt tccgtttcgc aacatgatca aatgatcgca gattttttct acattatcca
     4801 tattgtggct agaaaaaatg acacatgaac catgtttttt caattccaat attccatctt
     4861 ttagcaattc agcatttacc ggatctaacc cgctgaatgg ctcatccaaa atgatcaatt
     4921 taggttcatg gattaacgtg gcgatcagct ggactttttg ctgatttcct ttagacaaag
     4981 attttacttt atccgtcttt ttccctttta cctgaaattt ttccatccaa aaatcgattt
     5041 ttggttcaat ctcttttctt gatttccctc taagttctgc aaaataaagc agttggtctt
     5101 gaatcgttac tttcggataa agtccccgtt cctccggaag ataaccgatg acatcataat
     5161 ctttctcccc aagatgatgg ccattccata acacttctcc ttggtcttgt gtcaaaaaat
     5221 ccaagatcaa acgaaaagtc gttgtcttcc cagccccgtt ttggccaatt agccccatga
     5281 tttttccatc ggggatttgg aaagagacat gatcgacagc tgtgtagttc ccaaaagttt
     5341 taacaagatc cttgacttct aacatttctt cactccttct tcaagaaatc acatatatcc
     5401 ttagacacca gacgtaagcg atttattatt taccaacaaa acgtaaacgc tctttttgtt
     5461 gtacataaaa tgataggaca ctttactttc tttgtaaagt gccctttcct cattcctgat
     5521 gtaatttatc aaatacgttt tgtgcgacat ttttcggcag tccgatttct tgtaattctt
     5581 ctgtcgtggc tgctgtgata tttttcaacg atttgaactc tttaagcagc attttttttc
     5641 gttttggtcc taacctttca atttcatcta atcgggaagc aaaactgttt ttactgcgta
     5701 gctgccgatg gaaagtgatt gcgaatcgat ggacttcatc ttggattcgc tggagtaaga
     5761 aaaactctgg tgaatttcgc tcaagcggta ctacagccaa atctataccg aatagcaact
     5821 catttgtttt atgtttatcg ttttttgcca tgccagcaat aggaatatcg attcctaatt
     5881 gattttctaa gacatctttt gccacatcta cttgtccttt tcctccatcg atcaagatca
     5941 aatcaggtaa aggcaaccct tcgcgaatca ctcgtgaata tctgcgatag atgacttctc
     6001 tcattgatgc ataatcatcc ggtccgttga ctgtttttat tttatatttc cgatattctt
     6061 ttcttgccgg ttttccatca atgaatacga ccatcgcagc cacaggattc gtccccatga
     6121 tattagagtt atcaaaagac tcgatacgta ttggggtcgg gatattcatc gcgtttccta
     6181 aacgttcaac tgctccgatc gtccgttcct gttttctggc aatcagatca aaccgttcat
     6241 tcaaagcaac tgaggcattc ttattcgcca attcaactaa ttttttcttt tctcctcgtt
     6301 ttggttgcag cactttcgta gctaacaaag cttctacact gggcttgtcg atcgtatcag
     6361 gaatcaagac ttctttagga atgaaatgct cattttcctg ataaaactga ccgaggtaag
     6421 tcaagaaatc ttcttctgct tcgttataga aaggaaacat tgaaacatct cgttcaatta
     6481 gctttccttg tcttacgaaa aacacttgga cacacatcca tcctttgtca atcgcataac
     6541 caaatacatc tcgatccagt aaatcagtat ttgtcatttt ttggcgagtc atgattgtct
     6601 caatggcacg gatctggtct cggtattctg cagctttttc aaactccatg ttttcagcag
     6661 ctttttccat tttctgatgg atctctgact cgattttttc gtgtcctccg tttaaaaact
     6721 ttttcacttc atcaacgatt ttcgtataga ctttcggatc tacgtcgaaa taataaggac
     6781 acagacattg tcccaaatga tagtaaagac aaggctcctt cgtctgacta ggtttgcatt
     6841 ttctcagagg aaataatcga tcgaggatct tctttgtctc attcgccgca ccgacatcgg
     6901 gatatggtcc aaaatacagt gctttgtctt ttagtacttt acgagtaatc accaaacggg
     6961 gatatttttc attcgttatc ttaaggaaag gataggtttt atcatccttt aacataatgt
     7021 tgtatttagg atcatttttc tttatcagat tgatctctaa gagcaatgct tcgatattcg
     7081 attctgtaac gatgtattca aagtcttcga tttcactaac aagccgttcc gttttcgtat
     7141 tatggctgcc agtaaaatat gagcggacac gattttttaa tattttggct ttgccgacat
     7201 aaatgatcgt tccattttta tctttcatca agtaacagcc gggctgatca ggaagtaaag
     7261 ctaatttgtt tttgattcgt tcgttcatct ggtactcccc ttcatattca attaaagtta
     7321 ttatagcatg tctgtcaaaa aaacaatcca gcctgacaac gtaattatac tcaaaaaata
     7381 ttaatttgaa taaccttaac tcaaccgcaa aaaaaccgag gacggaactg gattcagtcc
     7441 caccctcagc ttatttttgc tgtaagtttt tataaatatt gatcgatcat ttgacgtagt
     7501 tgttcttttg aatgaacacc aacagctttt tctactactt gaccatcttt tttcaacatt
     7561 aatgttggaa tgctcatgat accgaattgt tgaggagttt ctggattttc atccacatcc
     7621 attttagcaa ttctgaattc ttcttcatca tattcttgtt ccaactgatc caaaatcggt
     7681 gcttgcatac gacatggacc acaccaagtt gcccaaaaat caatcaatac aagtccttta
     7741 tctgtttctt cattaaatgt cttatctgta attacttgtg acatatcaaa gacctccatt
     7801 tttaaaatct atgattagta tagcatttct atacttggat agcgaaccat ctgcttacct
     7861 ttagtaagct ttacttaaat ttaacaattg tcgcaccatt tccgccttgg tttgccggtg
     7921 caaactcaaa gcttttgacg ctccgatggt ttttaagata atcagtaatt cccttgcgaa
     7981 gtgcgcctgt tccttttccg tggacaatag tcacttgagg ataaccagct agaatggcag
     8041 aatccaaata ttgatctacc tcacttagcg cttcttcgta tcgttttcct cgaaggtcca
     8101 attgattcgg cacgtgacta ctagattcag atcgtacagc atggataact cgttgtgtcg
     8161 gctctttttg aggagcaaca ggcgtcagct cgtcttcgga cacattcatt tttaatatac
     8221 cgatttctac ttgccactga ttcccgtttt tacgtaaaag cgtccctctt tggccgtaag
     8281 tcgtaaccaa cacttcgtct cccggtttca acgttttttg ttctttcgcc ttcttcaata
     8341 ctttattttt cttcaaagtt tcttcttgat gtagatttgc taattgggtc tttgcatcga
     8401 tcaactgatg ctctttcaca tttccttgcc caatctgctg ttgcatttta cgaatatcag
     8461 caatgatttt ttctgctttt tcttccgcct ctgacacgac ttcgtttgct tttttctttg
     8521 ctttttccat ttctttttca cgttcttcaa agaaatagct gtaagcttct ttcaaatcat
     8581 cgtgaagttc ttgtgcctca gaaacaaaat gacgcatttc cagatattcg gtctctgcca
     8641 ttttccggcg attctctaaa tctgtgatca tttcgtttaa atcttggctc tcatcattca
     8701 tcaactgctt cgcttcatca ataacttctg tatcaagacc taatcggcta gagatttcaa
     8761 acgcattgct tcgtccagga acgccaatca gcaaacgata cgttggactt agtgtatcca
     8821 catcgaactc catgctggca ttaatcgtat ttgcccgatt gtatccataa actttcaatt
     8881 caggataatg cgttgttgcc atcacataag cagacttttt accaagatca tctaagattg
     8941 cgattgctag agcagcacct tcttgcggat ctgttcctgc accgagctcg tcaaataata
     9001 ccagactttt ttcattgact tttgataaaa tatctacagt gttcgtcata tgagaagaaa
     9061 aggtcgatag actttgctca atcgactgtt cgtctcctat atccgcaaat acttcctcaa
     9121 aaataccgat ttgactttct tcacctgcag gaatcggtaa tcctgcttgc cccatcagtt
     9181 gtaacagacc taaagtcttc aacgtgatcg ttttcccacc tgtgttaggt cctgtaatga
     9241 cgatggcttg atagtctttt ccgattgtga tgtcattaga aactactttt tcctgatcga
     9301 tcagtggatg tcttgcttgt ttcaagacga catgattgtc ttcactgatt cccggcacga
     9361 tggcttttac ttctttacca aatcgtgctt ttgcattcat cagatccatt ttaccaatca
     9421 cataagcatt gtggatgatt tcttgacgat ggggaacaag ttcagcagaa agttcactta
     9481 aaatacgttg aatctcattt cgttcagcga tttggtattg acgcaaacga ttattcaaat
     9541 ccaccacttg ttttggttca ataaataaag tctgtcctga agcactttga tcgtggacta
     9601 caccgccaaa tacaccgcgg tattcttgtt tgactgggat aacgtagcgg tcatttcgca
     9661 tggtgatgat cgcatcactt aaatactttg catttttccc acggacgatc ccatctaact
     9721 gttcacgaac agcttgttca cttcgccgaa tgttctgtct tatgctcttt aactcaggag
     9781 aagcgtcatc agtcacgcgt ccgtcttcat cgattgcttc ctttaaacgg cggctcaaaa
     9841 cagggatcgt gactaactga tctgcccaca tatacaaacg tgcaaattcg atttcactgt
     9901 cagaaagatc atcaatgaaa cgattcaatt cgcttgtcgt agacaatact ctggaaactt
     9961 gtgcaagctc cactccattc aagtctgctc cgatctcgat ccgtttcata tggggacgga
    10021 tattttcaag tttaggtata gggatcccgc ctcgcagacg ttgcactttc aatccatcct
    10081 cagtttcttg aagccagttt gtgatcgttt gtttctcact cactggtacc aattctgcca
    10141 gttcttcttt tccttgtgca gtcactacaa attgtctgac catctgtttg actttttcaa
    10201 attccaatgt ttcaaggatt cggttattca ttttatcacc ttatctttca ctatgaaaaa
    10261 agagctgtgc cggacatacg ctacgcagcc ttcttaaata aactactctg tacaaaaaac
    10321 gaaatatttt ccagcactat tttccttaat tcaaaatatt cgttacccaa agctcataca
    10381 tcttgttggt aagaaatggc gttctctcaa cgataaaacg agcgaaacta tgctcgccga
    10441 attgattttg gaccacacta atggaacaaa ggcaagtaac tgcaaaatca aaaatatagt
    10501 aacgtaagcc atgattagtg acaggatgcc tcctgcaatc cagtctacct gtctcaagat
    10561 cggcacatac gtcaatccat ggacaaaaac ccccacaaat cgggtaagca gccagccaat
    10621 tagcaaaata atcagaaaag cagttcctgc ataaaaagct tcatctaggt gaaaagcaaa
    10681 tacctgatca aaaaatgcta actttgatgt tggggtgaca gctgggtatg gaatgtataa
    10741 ttccaaatga cttgctaatg gtttgtaaaa atgctgggca gcaataaatg acaatacata
    10801 tccaatcgcg aatatgattt gcaaagcaaa cccgcgacgt gctccagaaa aaaacgcaat
    10861 tagcaataaa aataaaatca gcaaactcag catgctacgt tcctttctct tctaactttt
    10921 ttaaattttt cttattttga taaaaggatc ttaattagaa gatttttgtt taatttgtcc
    10981 ttttcgtttc tcgtttaaaa tctgttgtgc ttccacatga tttttaatgg tatgatctgc
    11041 ttgaccattt tctttcaata cttcccgagc ttcgttctcg attgcttcaa tgcgtttcac
    11101 acgattttca agctctgtaa atttgatcat ttttttcttt aattctgctg tttcttcttc
    11161 cagctccaag attctttctt gtttcttcaa ttgatccgaa agcgcattga cagccatcaa
    11221 gattgctgct tgttcattat ccatttgtgg tgataattgt ttcaattctg ctaactgttc
    11281 attgatcagc ttcgttacga tatccatatg atgcttggtt tcccgtccga taatagtata
    11341 tgtttggttg gcaatcactg ctttgtagcg tgttttttcg tgcgccatac gaatcatttc
    11401 ctcctatttg atgaaagttt tatttagtgt accatgaagt tggttctttc accgttctcc
    11461 acaaatattt ttcacatact taactattat atgataaact ttaaaaaaat gctatgtctc
    11521 gcaatctctt agaaaacgaa aagtcgtgag ttgcggtata gttttatgta taaaaacgag
    11581 gagaatttta tgagcaacgt cattatcaaa gcaacaaacg accaacttag tcaaatgaaa
    11641 gcatactatc gctcttattt gttaaataaa cagattcctt atactagctt tgctgcaaaa
    11701 aaagacggga cgaccattac cgcttataca tccggaaaag tgatgtttca aggtacgaat
    11761 gcagcaatag aagctgataa atggggggag gcacaggata aacaagccaa gccagcaaaa
    11821 acagcctctt ctttgccaaa aaatatcagt caattatccg ttttaggtag tgatgaggtc
    11881 ggaaatggca gctattttgg tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag
    11941 cttgcagaat tacgtaaact tggtgttaga gattcaaaag agttaaaaga tagccaaatc
    12001 atccagcttg ctgacatctt aaaaaaaacc atcccctata aactgcttgt cttatctcca
    12061 gaaaaataca atgagatcca accaaaatac aatgccgttc ggatgaaagt cgctttgcac
    12121 aatcaagcga tccatttgct gctaaaaaaa atcgagccag aaaaaccaga agcgattttg
    12181 atcgatcagt ttacgccgga agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa
    12241 ttacaagaaa aactgtactt cgttactaaa ggagaacaat atcatttagc tgttgctgct
    12301 gcttctatca tcagtcgtgc cagctttttg gaagagttac acaaagcatc agctgaagct
    12361 ggaatcaccc ttccatcagg agctggaacg aaatctgacc aagtagcggc aaaactattg
    12421 gagaaaggcg gcatgcctat gcttgaaaaa tatgccaaac ttcattttgc caatacagaa
    12481 aaagctttga agctgctaaa aaaataatgg aactgtgaca aaagtcatgt cacagtccct
    12541 tgttccgaat aaacggtgtt ctcatgccaa ctcctcggtc acaagtcaca atagtcatca
    12601 aacatgagaa gctgtttttt gaaattgctc cttgtgacgc ggagttaaac ggcaggctca
    12661 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc gataataagc ccggccaaac
    12721 gaaaaatatg aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt
    12781 caatgcttgt gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt
    12841 taaatcacta tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc
    12901 tcgaagaact tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa
    12961 ggagtcagca attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat
    13021 tccagttgct tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc
    13081 ctttttttct tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg
    13141 ggtcaaataa accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact
    13201 taatgttata tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc
    13261 gccatgccca ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat
    13321 attctttgtg actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt
    13381 cttgatctga taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat
    13441 agttgcaagt ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat
    13501 tttgattaat gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact
    13561 gttcgtactg ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa
    13621 acagataata atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga
    13681 ctctcacctc gtagatccag actaagggtc aaaacttaaa atcagcttaa gtacgcaaaa
    13741 aaggggctgt aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca
    13801 tttttggcat atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa
    13861 tagtttcctt cattctacaa acaattaatt tcttcgttat taaattaaaa cacttcctgg
    13921 aacaccgaca taataaataa aaatataccc tgataatcat gagagaataa caaatttgta
    13981 tttatgaaaa actacctata taaaaaaaac aagatataac gatcttcgtc atatcttgct
    14041 tcttcaccta actatttttc tccctatata acagctttac gcttctccta atacaccatt
    14101 caaatataaa tcgatcaatt gcgccacatt tagcggttct tccacaggtc ttttcgaccg
    14161 agtgatgtaa ggtgctaaca ctaagaaaaa atgccttgct gcgatttctt ttgaaatccc
    14221 atttcttagc acagattggt ttgctgaaaa gatttcttca aaaggttcac gataattttt
    14281 cttccataga aagaaaagat gatctcttgt ttcctttgaa agattgcttt caagatcgtg
    14341 aagcatcaga aacaaatcga ccagatgtgt ctcaatcaaa aactgcgcca tttttgtcaa
    14401 ggttacttga aatcccgttt cgcttgtttg aacgatttct ctcaaagctt ttccaacatc
    14461 ttttaagtgg ttttctaaaa ctgccgtata tagtttttct ttatcaccga aataatgata
    14521 gagattgggt tgggtgatcc caactttttg tgcgatctct cttgttgaag tgccgagata
    14581 tcccttggac ataaacaagt cagtcgcttc tttcaaaatt ttttgctgtg tatctttgtt
    14641 gtatcgatgg accatttttc cacctgcttt ttggttattc tttttctttc aatacaccat
    14701 cttccatcat aaatattcta tcgcatttgt caatcagccg tgtatcgtga gtaaccatga
    14761 tcgttgcttt atttttgtct tttgattctt ttgccaagat atcgactact tctcgtgcct
    14821 tttctgaatc caaactagca gttggttcat ctgctagaat aatggacgga tcattgtaca
    14881 atgcacgagc gattgcgacc ctttgccgtt ctccaccaga tagttcttct ggatacttgt
    14941 tgatcaactt atccacgcca agctgctcaa ataattcttt tgcttctttt tgacgatgtt
    15001 catgtttgat cttatctact aataataact gcttttttac tgtcagaaaa ggaaccagat
    15061 tggaagcttg caaaataaag ccaatttctt taaaacgtag tttaacgcgt tcactttctt
    15121 tttgattact gaattcttga tcattgataa atacagagcc actgctaggc gtctgcaatc
    15181 cgcctgccaa agtcaaaaaa gtacttttac ctgaaccaga aggaccgatg attgcaacaa
    15241 attccccttt atcgatactc atggttgttt ctttcaatgc tttaactact tggtcgccat
    15301 cttggaaact tttttctact tctttaaatt ctattgcttt catgctcttc ctccttctta
    15361 tccaattgcc ttcaacggat cgatttttac gattgtccga acagaaaata gtgcgcctaa
    15421 cacagctact accagcatca acaagctaat gacgccaaaa aacagccaat tgctttggaa
    15481 tggaaccgca tcaggtagaa caagggatgt tcccactgtt cctaatagac caagaagaat
    15541 accaacaaat gataaaatga atgtttgtgc cactacagaa cgcgcaataa atcctcctgt
    15601 gatcccttgt gctttcatta caccaaagat attaatcttt tgcatcgtca gtacataaat
    15661 gaaaatcccg atcacaatag ccgcaataac gatcaaaaat ccgatcataa atccaaaagt
    15721 caatacttgc gcattatatc caggtaattc attgatgaat ttagaaattt tgatctgttc
    15781 taaatcttca ggtaaattac tgattttacc tctgacaact attgcgttaa tccgtgcatt
    15841 ttcacttgta tcttcttttt cgaagcggat ctcttggtaa gcattgatgg ttgtatagag
    15901 aactggtgag acattgaatt tggcatgatc cgtaaagcca gttagtttaa acgtcttgtc
    15961 actaccagat aacttaactg tatctcctac ggctagacca tattcttctt tcaaactaat
    16021 atcgcctact gcctcatcat cgttatcaaa agcttttcct tcaacaagat tgggcatgat
    16081 gaattgattt ttatcgatac cgaagaagct aacattgatt tttccttctt ctgtactgtc
    16141 tttactagtc acgactccag gtgtctgggc aagatacgcc actttcccac cttcgacttc
    16201 ttctgctatc ttcttagtaa tcatggacat ccctagattt gaatttgcat ctttcgaaag
    16261 cacaatcgaa tctgcctccc acttgtcgac tgctgttcga ttatcttgag ctaagccata
    16321 agctaaccca gtcaaaaaaa ataccaggta ggcgatcaaa aacatcaccc ctgcaactaa
    16381 cgcataacgt aactttgaat gcataatttc atttaatgct aaaaacattt aactcctcct
    16441 cctgaaataa tcattttata caacaatttt atcacttgat aaatttttgg cgaatattat
    16501 gctcaaaaaa ttcttccgct atttcattat atgaattttt tattctcgat tgattaatat
    16561 ttattatatc cccacacaat aaaagaccgt gatatttctc acagtctttt atctactcta
    16621 ttgctatttg ctctcttagc tagtacttta aaagctaact cattgaggta gtgaaaacga
    16681 gaggtgtgtt cagacagaaa tccaaaatcc tcaactgcta gctgtggaat cgtagttttg
    16741 tgtttcacga tatccgcaag caggttcaaa tctgataata agaattcttg gcttatcagc
    16801 tgtccgccaa ttatttctaa tgtatccgga tggtagatca gctttgcatt tactgttttg
    16861 ttttcttcat cgtattgtgg aaatagatca aattctcctg aacacgaacc ggtattttgt
    16921 tccaagaatg gcgcttcatc ttctgtcagc cctacagttc ctaaaaagta gtctataatg
    16981 actgctgctg ttatcttttg tctgaggtcg taagcggttg gctgaccact caaagttagc
    17041 gctactgctc ttgccatatg tacagctcga gtaactaaag gtaaaaagag atgccgttta
    17101 tttttgtcaa aactgactgg gacgatatct ccaatcgcat agatgtccgg atcacttgtc
    17161 tgcagatatt cattgactat cacagtctca tcctcataca aagccacttt ttcttttaac
    17221 aatgtcgtat taggacgagt ttgggcagaa aaaatgacca tatctgcctt tattgcttga
    17281 gatagaagtt gtacggatac tactttctct tctgattttt tccaatcgat caagaattca
    17341 tttggataaa aattgatttg cttagtttgc agctgatttt gaaaactgtt gatcatatcc
    17401 ttatcgaaat atcttgataa aacattcggc atataatcaa ctagatgaat aatgtatcct
    17461 ttatttctca aagcatctgc taattccaat ccaatataac cagcaccgaa gatcactact
    17521 tcttttgctt cttttaaagc ggcaagtact tgcttagatt gcactagatt tttataggtg
    17581 tagacatttt cagcttcaat ttgtttatac aaggtgacag gagaggaacc tgttgctacg
    17641 attaatttat cataggttat ggtctcgttt tttccttctt tggatgcgag gatgagctga
    17701 tgtttgtctg gatcaagttc agttactctc cattcggtga tcagttttgc cccactttct
    17761 tgaagagtcc gcatgttatg ggcaacttca gctaaactac gtatctttcc ttttaaataa
    17821 aggttgattc catttgaaac atagcctagt ccatctttat gatcttcatc gatcagtaaa
    17881 acttctgctt tcggttgaag ttttcttaga tttagggcag ctgtgatgcc tgcatgggaa
    17941 gcaccaatga tcacgatttt catgttttat tcatccttaa attcaatttt tctaaataac
    18001 attcttttaa aaattggttc aaatagggga aatcataatt atattcgttg ctagtgaata
    18061 caaaaacttt agcggacttc tgtgacgggt actctttcaa ttggctagtc aaaatgatat
    18121 catactgctt ttctttctcg tattcgacta aatcatattt gatcgtattc agcaattgtt
    18181 cctggatgat ttctttcacg atatccgcca ttagcctttc ataaggaaga tcataagcaa
    18241 tttgcagacg taattctaac tgcttgatca gcgtccgcaa aatagatgca tagcgagcaa
    18301 ataattcaaa gcgggcattt tcctcgtcta cttcagtaaa atcaacaata gcaaatgttt
    18361 gtctgaccaa tctttcacat aaaaccagga attgtttatc ttctacttca gttaaccgct
    18421 gttttaatcc ttcttggcca aaaatattca tccatcctct aaaataggca aaatgagcgt
    18481 gattttgcat cactgtcaac gcaatctttt gcgaaatctc ttctggaaaa acataaaaag
    18541 agaaatattc ttctatcgat tgtttaaacg ttcgattcaa ttgagctact acgtcgtctt
    18601 tgtatttttc gcttatcaaa aactcctgga catgcgagtt atgtgtatct aatgtaaagt
    18661 tactgatcat gaaaagataa aaaaccaacg tttcattttg attccaagga aaggcatatt
    18721 gaaagaagta acgggtcaaa aatgtatgga ggtccaataa aaattgtgtc gtttgaaaca
    18781 atccaacagg aagattgcta taatctggtt tggatttttt cgtacagcgc attcgcttct
    18841 tcataatgga aagccatatt tcaaatttca atcgtccttc ttctgtaata gtaaaactaa
    18901 actctttttg cagcagatct aaaaaagtgt ttcctgataa atcactgtat tcttttttgt
    18961 tttcaagata aggacgtcca aaccaaaata attggaaata aaaataacaa atttgacttt
    19021 ctggaccggt cagccgcccg ctttttattt tcagatgaaa ctcatttaaa gaatcattta
    19081 attccttcac tctgcgagac aaagtagccg aactgatatt aagttccatg acaagtttct
    19141 gccgatcaaa tgagccattt tccaacagat aaagaagtat ctgatatttt gtagaatagc
    19201 gcaagtagca atgataataa acatcgactg ctgctatatt tccatgtctg attaataaaa
    19261 tattcccttc ttggataaca atttccaatt gtttgatatt ttccaagtta gcttgtaaat
    19321 aaaacagatc ttctttcaag gtagcccgat caactgataa gcgggtaagt acgttattaa
    19381 cggaaatctc tcctttttcc tctaataaaa gttggaaaag tttgatttgc cgttgttcat
    19441 ttttttcaag caaactgaaa agttccaagt ccactcctcc aagcatttct gattttaaca
    19501 gcagaagttt ccttctcatc cttgaaaaag aggatctgtg acagttatca cagatccata
    19561 caaattctaa tcacttagct ggttggccca gctgtcagtt cccatgtgac catgccttga
    19621 tacgttgcgc caggttgagc attcgtactg ctatttacct ctaagagtaa accatcggta
    19681 tcgggaaagt caacgagaac ctctccttta gctgttccat cactctcata aatcaacgtg
    19741 ttttgacctt gctgaatcaa ttgttttgat ccagaatgat tgaagtataa ttttgttccg
    19801 cttaattctt gaccttgatc attcttaaat aaagaagtca atgtccctgt cagttgccaa
    19861 ggagacgtgc ttggaagccg agtatcccgc acatcaaaag aaaaatcgcc ttttgcttca
    19921 taataacttg tcttcaacgg tttggggaga tcgttaccaa atgtaaaatc atctggtaca
    19981 ttaactaatg ttaattcttg tttggggaca agaattgttt tagcagatgt ttggacaact
    20041 gggttaggaa gcttagcacc atctgctaat gtaatattgc tggaaagtac agctgccttt
    20101 gtcagtatgc tgacggattg tacagaagta tctacagtat ttaattgata gttgtaagta
    20161 agatgaatgg tatctcctat tttgctcaat agatctgctg ggacagaaac tccttgttga
    20221 atgccggtat ctgcgatagt tgtaccattt aattttaagg aaccattgat ataagtagta
    20281 ttttcaggaa gtgagatcgt gatttttttg tcggttatcg catccccaga ttttgttaac
    20341 gtgaatgtca cattttcttg aatcgtatct cctgatagag cagacagggg atctcctgtc
    20401 tcaggttgtc tgctaaggtt ttttagctga ctatctgcac tcacagatgg aaaattagcc
    20461 actgttattt gaggaagagt ggtgattcca gcttgttcag actgccattt gtacgcaaaa
    20521 gacggataca tgttagtaag tacatcgtcc accaagcgat aatcaaaata atattgccct
    20581 gaagccaatg caggtagtgt agaagtagct gtatttacac ccccgttatt tgattggatc
    20641 gtttggaaaa gttgatctgt ccatcctgtc gctaattttt gggttccttt cgaataaagc
    20701 ctgtaatgga tcgaaccctt ttttagttca gttgctgacc aattggccgt gacttggtac
    20761 gatgcagaag aaaggagctt tccccactga ctatcggctg ttaagtttgt tggtaaaacc
    20821 gttccgtcta gcttgtactg ctccaatacc tttaaagcaa caggagaaag caatccgcct
    20881 tgcgcatctt tcccactgta cacgatattg gctgtctgat tcaatccact tgtgcttgct
    20941 ggagtatgag tcagtattgc tccttgtgta acgttttttc ctgtaaaata gttgactacg
    21001 cgatcaaagc gactaaatgg caactgatca aaacgtcctt cagtcagccc catcgtcaat
    21061 gtccgactat tggtagagcc tggaaggttc tggttatttc catccacgta ttctgtggta
    21121 tcagcaaatg tattatattt tcctgttgaa tcacttgttg agatgtaact attccacgta
    21181 tttatctctg cacttgcaga aaatgcatat gctcctggat tcaagtttcc gccagttaat
    21241 ttaggataaa atctacctgc agctggagct aaaatcgaat tgaacatacg atcttggata
    21301 atattattcg aatcataaaa atcaacagaa aaaacggcct ttttagacat agaaggttgt
    21361 aatttaccct tggtcaaaat tttgtttgga ctcgtatcat atttatctcc gttttctgtc
    21421 atcttcgcga cattagctgt aacatcaaat tgaagcggga tatcgtccac gttgaattcg
    21481 cctcgcgtaa taatagcaat aatatctgcc aatagagtat ttgtatccca cgaccccatc
    21541 ccaaaacgca aggtatggct tcctgctgat aataaactac cgctagtaac cttgatgggg
    21601 gcaccggcaa tatacaagta ggcgttgttc cagtcaattg cttttaatac atcactgctt
    21661 gatacatgtt ccggcaaatt gatgatcgcc acaaatgcaa atccttgtgc agtgatattc
    21721 accaaatcaa gtacatttgt tagtttcaca tggctttccc aatgaaaacg gaccgtgcca
    21781 cctttccaag gatcatttga accatcgttc aaataagtag cagtcaattt aggcaattgg
    21841 ttagccccag ttagttggac aagcttcccg ccattgactg cactacgtcc accatttgga
    21901 ggtacgatat tttcatacgt gtatggatca taagaaatgg caggaggcgt tgctccaaca
    21961 gcttctgccg aaggtgaaaa gataaatata cttaaaacaa aaagcgaagt aaaaaagaag
    22021 gagcgaaaaa atgaaaattt attttgtagc aacatgttca tcctcctttt cctttcttct
    22081 ccataacaac aaccacgcaa gaagccataa ttctatacca atgatcaaat aaagaaaacg
    22141 gatcttttct tcattcgtct ccgggaatac gccatgttga ttagattctt tataaatcgg
    22201 tagctgtgtc tgctgttttg atgcgccatt gactgaacca cttggatgcg gaactggact
    22261 tagatcataa aatgtaatcg tacctgaact tttggtatct gcttggactg gctcttgcca
    22321 gctgaagaac agcaaacttg caataatccc tcccaataag atcgatgttt tcaacgaaat
    22381 cacctcatca tgttgtttct ccttttttct ttttcttgtg gacccacaaa aacagcaaaa
    22441 tcaaccataa cactagaact gcagcaacca atcctgcata aagccatggg aaagattgtt
    22501 cttgtactgc aaaaacggaa ttttcattta ctttttgtgc tttttctttt ttgatcgtaa
    22561 agttttgtgt aaatcgccat tcattactgt cgcctgattt tgctatgatt tctgcgcggt
    22621 aatcgccggc ttttgcttta tcaccagcta aatcaagatt caagtgaaaa ttcgaatttg
    22681 gtgcaactaa aaattcatta gacgtatttt caatataaag ctgattttca taaaagatct
    22741 tgctcgtaaa tatcaaattg ctgataatcc taggctgagg attttggaaa cgaagctgaa
    22801 cagttggctt tgcgttgacc tcgtccaaat ctcctgataa aagtttcaga tccggttcta
    22861 tcgtctcttg actttcttgt aaaacgactg cgagattata agcaatacga tttttgattt
    22921 gtgctccttc tgtagcattt tctgtttcag cttcttcttt aggtgaaaca tgaatacccg
    22981 ctaatacacg tcccttaaag ctttttgctg ggattttcac ttctataggt atagttgttt
    23041 ctgcatgtgc tgatacattg atttcctttt tcgcaatgag caaaacgtca cggacatcaa
    23101 acggcaatgt ctcatctaac gttgggtttt tttgactata atcgatcgtt ccaccatcac
    23161 tcgttaccgc cggattgaca gagacctcaa atgttcgttc ttcatctgaa gaattgctga
    23221 taattacttc tagtgtctga ttttgttccg gtgccactaa taaatcaaaa taaccgattg
    23281 ctttatctat ttggttctct ggaaataccg gtttgattcc aaagtctcct gctgaatcct
    23341 ttgcttggct gattgacgga aaaatgaaaa atagacttaa aatagaaaaa aacgaaacaa
    23401 acagtcgttt actttgattc attgtccacc acatcctttc aacaacaact gcggtaaaaa
    23461 atccttcacc tatagtattt tcaccgcagt ttgttgaatt tatttcatac tctatgctac
    23521 tgggccagcc acgaggttcc aagtcaaatc agccgtgaat gttccagctg ctgctttcgg
    23581 cactttcagt gtgatatttg ctggatcata aacagaaagc catgttccca gaccttgatc
    23641 ttttgctgct gtaaatattg gtgtcgctgc acttgcttta ctcaacccag ttacagtttg
    23701 actagttggt ccagtggatt cgttatctga cgtactttta acagtggatg ttccaaaatc
    23761 taattcagcg ccttgcaaaa cttgtgaacc gtttacaaaa tctgaaaccg aaactgtcac
    23821 gttccaccct tgtgcttggc catctgcacc tacacctcga cgatctgata cttggagata
    23881 aggtgtatcg ttttttgaag cgtctacttg atacgttttt gttccgcttt caatctcgtg
    23941 tgtaccaaat ggcaattcag gaactacatc caatgttaaa gaacctgtct ggccagttgc
    24001 tggattttcc ggatctgctg gctctactgg ttttgaagga tcagtagggt taagaggctc
    24061 aacggtattt gttggttcag taaaggtaat atctcctttt gaagtcgctt gaccctcagc
    24121 aaaagctggt gttgccaagc cgcctagaag aagtgttgaa agtaataatg tcccaatcat
    24181 gttctttttc ataataaatc actccttttt cttagtgacc taagtatagt agagacagta
    24241 tgcaaaaaat cctcatgaat tcatccgctt acttgaaatt atgacccttt ttgtataaaa
    24301 gagaaaaaaa caataaaatc gtttacatga atattcttcc ttttttatta agtatgaaaa
    24361 aatatttcaa ttttattttt tcgtttcaaa ttattctatt agtgacatcc ttgaactata
    24421 aaaataggag tacggcatca tctgccgcac tcctattttt ttcgtcattt atttcttaaa
    24481 taaaacgatt ctcacgcaat tttatttttt tgatatcttc tactgtttgt gttccagcta
    24541 attgcatgac catttccaat tctgttttaa agaaatcaaa cacttgatgg actcctgtac
    24601 ttcctcctaa agacaatcca taaataacgg gtctgccaat agcaacaaga tcggcacctg
    24661 atgcgattgc tttgaaaaca tgttgtccac gtcgtacacc gctgtcaaac acgattggta
    24721 cacgtttatc tactgcttct gccacatatt gcaacgaatc aaacgcagca ggccccccat
    24781 ctaattgacg gccaccatgg ttagataccc aaattccacc agcaccagat tctaatgatc
    24841 gatagacatc ttcttctgat tgaacacctt tcacgtagac tggcaaatca gaatgagcag
    24901 cgataaattc tacatctttt ggactaagtt tttgtttgga agatttatat acagcatcca
    24961 tcgtttggcc tactcctgat tgataagcct gaacgatcgg cattggcagc gggaaggtaa
    25021 acccgtttct gcggtctgtt tcacggtttc cgccaactgt tgcatccgct gtcaatacga
    25081 ttgcttttgc tccattacgc ttggccactt caagaatatc caaattgatt ccattatctt
    25141 tgctcatata aaattgaaac cattgcggtg ctttctcccc gcctgcttct cttatctctt
    25201 ctaaagtaca agaagcgtaa gaacttgctg tatagatcgt cccaaaatct gctacacctt
    25261 ttgcagaagc tttctccgcc ttcacatgag ctaatccatg agcagcaact ggtgccatga
    25321 tgattggtgc agtcagcatt tcttcgtcaa aatgtgtagt tgtatcgggt aattctacat
    25381 ctcttaatac atgaggaata atcaaacgat gattgaatgc ccgttcattt tcttgataag
    25441 taaataagtc gccagcacca ctgctgatgt aaccataacc accagctgga atcacttgtg
    25501 aagccgcgat ttctaaatct tccatattga taaaatctac tattccttca tttgtacctg
    25561 cttgatatgt tttttccatc tcaatgcctt ctctctataa aatttgaatg atgaacaata
    25621 tgatagcgtt tacacttttt atattactct tcgacattga tacaggcaaa taatctgttt
    25681 acatttcagc cattccagat gtcactactc tggaagaaat gattgtaata aaaatagata
    25741 aaaaaccgat gggactttga caaaagtagt gtcaaagtcc ctttttttga ataaacggtg
    25801 ttcaaaagcc attttctcgg tattaagtca aatttatgca aaaatgtgga aagccatttt
    25861 cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctct cgataaaacg
    25921 gtgttcaatc agctgaccct cggtattagc tcaaaaaatg gcaaaatatg aggaactatt
    25981 ttgccatttg tctctctaat actcaggtca gaacgttgac ttcacagtct cttccttaaa
    26041 cggtgttcaa aaacttgcat ctgcggtaat aagcccaacc aaacgaaaaa taggaggagc
    26101 tatttttgtt tggctgttct tattacttga tgcagggcag gtttttcacg acctctgatt
    26161 cacggtgttt ctttttcatc aacattttta cgaaatactg tggaagacta ttgaaataag
    26221 tgtggattgt ttctttgtgt ttcaatcgtt tcttgccatt cttcatttaa ttttggatta
    26281 ccgttttctt ccttgaactc ataaggtcct aattcaaaaa tcccagacag tatttgattc
    26341 acgatttcat atagttcagt gtgatcagac acatgattct tgaagatcat ctcattatca
    26401 ccacgtttga acctcacatt cgggctctca tgatgaacct ctatgcattt ttctcctttt
    26461 tttaactgac ggacttctga aaacatctca taaatatctt ctccagtaaa accttcagca
    26521 agcgccagac tgtccacaag accgcctact gtcagttcag agattattgc ttgattggaa
    26581 tctaatttta atgtatagct tgtcgcttct actacgccac atgcattgca accgccttct
    26641 gatagttcgc tttttctaat caattgaact tctttcattt tctcttcctc ctcttaattt
    26701 tctacttatg tatttttatc acctgacata ggtgactcct atcaacgcta tcatgtgctt
    26761 ttttcctcag aaatcctatt tatccattcc atctatccct tattcttcta cttttctttc
    26821 tctagcatat aagagatacg catagtattc aagcaaggat tgtcgatata aaaagaacaa
    26881 atactgctat tttgatttct tttggctata ttacatataa ataaaattta tcatacctgc
    26941 tctttttgac tatatcatca tctgacttct atacttcgtt tgtattaaaa cttaggagga
    27001 atcattcatg cttacagggt taaacttatt aaataatcct tttctaaata aaggaacagc
    27061 ttttacaaaa gaagaacggg caaaatatgg aatcactggt atgcttccaa gtactgtcca
    27121 aacactggaa caacaatctg ttcaagctta tggtcaatac ttaagcaaac aaacagattt
    27181 ggaaaaacgt atcttcttaa tgaatctatt caatacgaat cggacattgt tttataaatt
    27241 gatgggcgaa catttagttg aattcatgcc tgtcgtttat gatcctgtcg ttgcggattc
    27301 aatcgaacaa tacaacgaaa ttttcttgaa accgcaagat gctgcatttc tttcaatcga
    27361 cgaaccagaa aacatcaaag caagtttgaa gaacgcggca gatggacgag acattcgttt
    27421 gattgttgtg actgatgcag aaggaatctt aggtatgggc gactggggtg tgaacggtgt
    27481 tgacatcgct atcggcaaat tgatggtcta tactgctgct gctggtatta atccagcaca
    27541 agttttgcct gtatcgatcg acgctggtac taataatgaa gaattattga ataatccctt
    27601 atacttaggc aatcgtcatg ctcgtgtaga aggcgaaact tactatgaat tcatcgatca
    27661 attcgttcaa tcagctaccg aattattccc agaattgtta ttgcattggg aagattttgg
    27721 acgaggaaat gctgcagcga tcttagaaaa atacgaagat caaatcacta ctttcaacga
    27781 cgatatccaa ggtacaggaa ttgttgtcct agcaggtgta ttaggcggat tgaatatatc
    27841 cggtgaacaa ttgaaagatc aaataatttt gacatttggt gctggtactg ctggtgtggg
    27901 tatcgcaaac atccttttag atgagatgat ccgtcaaggt gtacctgaag aagaagcacg
    27961 ccaacatttc tatcaagtag ataaacaagg tttattgttt gaagatacag aaggattgac
    28021 tcctggtcaa atcccattcg ctcgcaaacg ctcagatttc actaatgctg atgaattgac
    28081 aaacttagaa gcagttgtaa aagcgatcca tccgacaatc atgattggta cttctaccca
    28141 acctggagca ttcactgaag aaatcgtgaa agaaatggct gctcatacac cacgtccgat
    28201 catcatgcct ttgtctaatc caacaaaatt ggcagaagca aaagcaaaag atttaataga
    28261 atggacggac ggaaaagcat tagttggaac aggtatccct gctgctgatg ttgaatataa
    28321 tggtgtaact taccaaatcg gtcaagcaaa taatgctttg atgtacccag gtttaggact
    28381 tggtttgatt gcttcaacag ctacacgagt aaatgctgaa atcatttctc aagctagtcg
    28441 agcattaggc ggaatcgttg atgtaacaaa accaggagca gctatcttgc ctccagtagc
    28501 aaaaatcact gaattttctc aaacgattgc tgaaactgtc gcaaaatcag ttgtcgcaca
    28561 aaacttgaat cgtgaagaaa tcacggatat caaagaagca gttgaatctg ctaaatgggt
    28621 tcctgaatat aaatctttag aagattaaga tcgttatagt aaaagaacat acagaaactt
    28681 tgtatgacgt ctaaaaagaa aaaagcgact gcggcaaaac agcggccagc caactcctgc
    28741 tcttttgtcg cttttctttg gcttttttca tatgatacag aataagaaag gatctgtaaa
    28801 aatggcacaa aaaacaacaa ctgcaactgc agttgaggaa aagaaaaaag aaggtttttg
    28861 gtccacaaaa atcaccggcg taagtttgcc actttatttg atcatggtcc ttgtcctgat
    28921 cattgcaatg gctttaggga aattaccgac gaatatgatt ggaccaatcg cagttttggt
    28981 tattttcggt aatttatttc attttatcgg gacaaaaatc cctatcgtca aaagttattt
    29041 gggtggtggt tccgtttttg cgatcttcgc ttctgctgcg attgctacat ttgggatctt
    29101 acctgaatat gtagtgaaat ccacagaaaa ctttgtcagc aatatgggct tttgggactt
    29161 ttatattgct gccttgatta ctggtagcat ccttggtatg aaccgtaatc tattgatgaa
    29221 agcttctgtt cgttttattc ccgttgcact tatttcaatg gtcgtctcct tttttgccgt
    29281 aggactcgtg ggcatgctta tcggaaatgg ttttgcaaat tccgttttat acatctcatt
    29341 acctgcgatg gctggtggtg ttggagccgg agccgttcct ttatcaacga tttatgcaaa
    29401 tgttttaggt acggatgctt cttctatcat ttcccgcctg attcctgcga cagcaatgac
    29461 aaatgtctta gctatcatcg gtgcagcatt agttgctcgt gctggacaat caatgccaaa
    29521 attcaacggc aatggtaaat taatggaaaa aggcgatctt ggcgatggaa aacctcgaac
    29581 tgtgaaacct tccattccac agttaggtgt tggtttgatg gtatcattga ctttcttcat
    29641 cttaggacaa attggtaact attttgttcc taaagtccat gcttacgcat ttatgattat
    29701 tatcgttgtc atctgtaaaa tcgccaacct attaccagag tattatgaag atgcagctat
    29761 catgttcaat aacttgattg tcaaaaactt gacagcagct gttcttgccg gtatcggtat
    29821 tgcgttgctg aatctgaatg tattagcttc tgctttgaca tggcaatttg ttgtcttatg
    29881 tctgacaagt gttatcgcga tttcagttgt ttcaggattt gtcggtcgtt tgtttggttt
    29941 atatccaatt gaatcaacta ttactgctgg cttatgcaac aactcgatgg gcggaacagg
    30001 aaatgtagcc gtcttatctg cagctaatcg gatggaactt attgcttttg ctcaaatggg
    30061 caatcgactt ggtggtgcca tcgttttgat catctctggt ttcttgatgc aattactttc
    30121 ttagtcaaaa aaacaaaaat acctgatcat caagtatcgg cttggtgatc gggtattttt
    30181 ttgataaaga tttacaggat tttcaaaaat cttttttgca ttttatcgga atgataaccg
    30241 atgtgttcat aaaattggtg tgcttctact ctgtattctg cagaattaag ccttattgtc
    30301 gttattcctc gttttttcgc ttcttcttct acgccagtca tcaaagcctt tccgatgcct
    30361 cttttttcag catatttaga cacagcaagt gccataatat tgaacattgg atcgctaaaa
    30421 atcgattcat aggactccgc ttgtacatag ccagctgctt ttttagaagt aggatcttca
    30481 taaaccaaga aaaaattttt ctctggctcc ttcagcagtt tcttcatttg tctcaccgta
    30541 acggttaatg gtatatcata ccctaattga tccgcattga tttcacgtag tgcttctgca
    30601 tcttctaaac gcatttcccg taacatccaa atcaccagcc aatttctgat tttttaaaat
    30661 gaattttttt atcatagtat actctttttt tcatttagaa caatatgttt cagtgatttt
    30721 gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg acaaaggcat tgtaacaatt
    30781 gaccaaaaga accacgagga caattacttg aaaaataaag gtcacttttt ttggagacaa
    30841 aaccctgcta aatcttgctc cccacaaacc tccaataaca gcagcgggga taacataaaa
    30901 caacatagat aaatcaaagc gcataaagct acttgttaaa gcaatcgtta ccaattttgc
    30961 cagttgagaa aagaaaatcg tagatagaga atatagtgtt gcttctttta ttggcaatgc
    31021 aaacatcagc atcaacaaag aaacattgac tggtccacca ccaataccta aaaaactagc
    31081 taaaaagccc agaacccccc ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt
    31141 ttggctgatg atgcttggtg tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa
    31201 tttggatcag ctgcacatgc ttctcgtttt caaataactg taaaaaaact tcgaatacaa
    31261 tgttgccgac tactcctcca acaacagctc cactagatac ccaaccgaca atctgccaat
    31321 tcagtgattt cccgcttgat acttgtcgca cagtcgaaac aatggacatc gtgaataccg
    31381 caactgttga gtaaaaagaa atccctgcaa cagaatgggc gccgataaga tcaagaatcg
    31441 gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc aactgtgttc gccagaacga
    31501 tcaccacaaa ataaatcaga ccaatcatct tgtatttcct ttctcttgtt tatatctttt
    31561 agcgattgtt actcttttct tttattatac agtaaaagaa acaaacgagg cttcgaaagc
    31621 ttttctactt tttaaggaaa tttagtgcta aacgaaaaga ggttgtgaaa aagctgtcct
    31681 gcatcaagta ataagaacag ccaaacaaaa atagctcctc ataattttcg tttggttgaa
    31741 cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg
    31801 ttcagctccg agtcacaagg aacaatttca aaaaacagct tctcatgttt gttgaatatt
    31861 gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagagac tgtgaagtca
    31921 acgttttgac ctgattatta gagaaacaaa tggcaaaata gttcttcata tttcgccgtt
    31981 tttcgagcga atgccgaggg gcagctgatt gaacatcgtt tatttggaaa aagggattgt
    32041 gacaagacgt ttgtcacaat cccttttttt atatcttatt aattcattct tttgttctat
    32101 tcttcttctt ccgccatgaa tgagaaaccc atatcaaaga actataaagg ttatcaaaac
    32161 agcgtttatg actaaatgaa ttagagttat ctatcactat ttttcaagcg atttaaacca
    32221 aatggcatac aatatggcat acaaaaactt caataagccg tgggggtttt taaatttctt
    32281 cttgttcttt aaaatcaatg agtggagcta cataatttga atccactttt tcaaacgtca
    32341 ctttttgcat cgacgaatgt tttctttccc aagataacgt aaatagattt gactctggac
    32401 ttataaagtc tacttcacct tctagtatta ctttcttatg caaatattcc tctaataaat
    32461 atagacttgc taaagcatac aacacatttt ccaaatttgc ttccttataa tttgaagaac
    32521 gaccatgttt tactctgtta tatattttcc accaatctgg tgtatcttca ggccacccat
    32581 ctccaaaagg ttttagtgaa gtcaatgtta aagaattcat aaccctaaca ccattatcca
    32641 aacacattaa ttcgctagat ttaataacag ctttaaaatc agttatgctt ttattgctac
    32701 tgctatcaaa tccgcaaagt tctttgaaaa taatctcaca ctccgaacca atcgctagta
    32761 gtagtttttg atattcaata gagaatgttt tgaaattatt ctcatcgagc atcacaaaat
    32821 ctgtggtatt aataaactca ttttctaaat gtagataata cttccaataa aaatttgtaa
    32881 actctgcttt gtccattaaa tcacctcttt tgaattataa caaaaaaaac acgtccacaa
    32941 cagacgtgtt ctttgatttc tacacaatca gcttgccaaa accatccaat cagtcctagc
    33001 atgcctgtat taaaattgaa cgttcatcat ctatcatctt tcttagtcca gcatgtttgc
    33061 aaccacacct acaattttca gtccaacaat agatttcgac tagctaagtc ttgcaaaatt
    33121 caggcagcta acccttcatt tactacctta attatactaa atacccacaa cgtttacaag
    33181 tgtaatcatt ctaagaaatc tgtttagcta tgatcgttgt ataatcttta aacgccgttc
    33241 cttcattgat tgaaataatt ttataactct tgtttttcca agctactttc atatcgtttg
    33301 ttagttcata atcttgatcg tatcgaataa taaacgtcaa cgttccttca agaatggtac
    33361 ctacactagc aagcacgtct tttagcattt gtgaacgcac attagcccaa cattcaaata
    33421 cagtttcttc actatccacc atctgcccat cttcatcttt tacccgtttt gtctttttaa
    33481 acttgactct ctcagtcaat tcatttacat tcttaatgat tggcataata agcacctctc
    33541 atttgttgta ctaatgcttg taccgttact ggtatccgtt cgctgctggc ttcttgacgg
    33601 ttcaaatacc aatgttgagt aagtaatgac accgccaaat caaattgctg ataattatcc
    33661 atatctttat ccgttgcgct actatcaata gcacttttta tatattcgcc tgctgtatca
    33721 atcaattgtt gaataaagtc atcatcaata gtgtgatcga tccgcatact tttttttatg
    33781 ctatccaaaa ttaccatttt atctctccta atgaaaaaaa ggcagtggga taaaccgccg
    33841 cccaatattt ttctatactt ttggtgttac aactggcgta aactcaatat acactgaagc
    33901 attttgatca attttcttat aatcgttgcg aacgatcact gcaagtcctt gtgagtagta
    33961 atcgaatttt tcccattgag tcgttacttg atttctacga gcaacaaaga tagattgtgc
    34021 aatatccccc ataatcatag ggaatgttcc agctttagga ttggcaaaca atgtatctgc
    34081 aatcaatact actggcatac cgaataatga tttaccacta ggtgctgtca catcgggttg
    34141 taaaatgtaa cgtccatctg aatctttcaa tgtatctagg tggtaatatc cgctttggtt
    34201 tagaattacc attttattta atgctgggtc taatgccaca ttgtatagtt gtttcaaatc
    34261 gtccaacgta gcagccgttt tcttagtgaa tgtttttaat aaatccatga tatgcttatt
    34321 gtcagtgttt tctaccaatt tcgctaactg atctttgacc tcttgtacaa tattcactgc
    34381 tgaatcttcc acaacctcat ttgataaggc aatcttacca gcacgagttt ctactttata
    34441 atcaactgaa gtaaacattt cagcgtcaat atcaccaatt tcagcaagtt cagcttttgt
    34501 tgctaacact gcttgttggt tagttgctac tggatattta ccttgtccat ttgatactgt
    34561 ttttactgtt gcatattgag ctaagttata atttgaacgt ttcaaatcaa agacttcacc
    34621 gattacttct tcgggaacaa ctgccgctgc atttacagta gtgactccat cacgcacttc
    34681 gccttgtgaa cggatatatt cttcataccc acgcaattct gttttttcgt tatcgattaa
    34741 agttttttcc atgttattac tctccttttg tgctttagaa gtggattcta agctattgtt
    34801 tgtttgtttt gcttggttgt tactcataaa cgattcgtaa gaacgcttat ttacttggac
    34861 atttgatgaa tcataagcgg gaacggtcac gacactaatt tcattaagtg ctttgatttt
    34921 atctattgat cgttcaattg tgccatcttc ttttttgtcg aatgaatcta atccaagcac
    34981 gaacccaaag gacatagaat caacaacccc ttttgagata ttctcataca catcatttga
    35041 ataggtcgtg tcggttagct ctgcttcaaa atgtaatcct gtatcatcga cattcaattt
    35101 caacgtacct gctttgacac tagctagtgg ctttgaataa tcgtgtccat ataataagaa
    35161 cacatttgat aaatccacct cttttaatgc ttcgggtgta atcacttcta caaagccgcc
    35221 taaatctttt gacggttgcc cgaatttcaa cgcatagcct gaaatagttt tgccgtctgt
    35281 tggttgtttt tcattttctt tctctacttt gggagaatcg gctgttagct cagcttcttc
    35341 tgtcagccgt ttttcctttt cttcttcatt attcaactgg tgtaactcct tcctgttctt
    35401 ttaattgata tttctccaac gtatctaaat acgtaaagtt caaacttgct agtaaacgat
    35461 cgccaccatc catggggggt agccccattt tcgaccgccc ctcattaatc gtgagaagtg
    35521 accctttgac ctgatccaat acatttttta cttttgtttc aggatcagtc tctagtaact
    35581 gatcggaatt aaagcggata ttggtttcaa gtttcgtatc taattcactc acaaacacat
    35641 taaagtaatg aatcagcgta ctttggatat actggagatt actttgaacg gtgctagaat
    35701 gttcattttc aacgcctaaa cgctcaatcg gtacaccaaa cgccttggca atttgttttg
    35761 tgttccaatc attggaattg ataagcttca aaacatcggt gtttatttct aatgttttgt
    35821 agtccattgt ttcatcaaga atgatcgttc taagagcatt atctccacta cttgaaccgt
    35881 tggcttcttc aaacttttca cgaattgcgt tctttgctga tccgtctaaa tctgacttat
    35941 gaaccttcaa aatacctgac ccactgacac cacgagtaaa gaaattgtgc aatgtgcggt
    36001 tgccagcttg ttgaattttc agttcatcac gtaacgcata aagtggtggt aatcctgtta
    36061 aaccatcttg ggtgaaatat ttgaagtgca aaatatcgct agacttcaca cgtctttttt
    36121 tatcaccaat ctgataagac aacgtaccat tatctaattg agtaaccgtt acacttgagt
    36181 taggtaataa gtgaatttct tctacctttt caccgttgcg tttaatctct gcaaagctat
    36241 taccgtttag caacatattg accgccaaag caaactttaa atgccaacca tccatttctg
    36301 aattaggttt ctcgtttagt aacttcacca gttcattatc agcttgtggc atattgtttt
    36361 tgaccaattg aatcggactt gaagcaatat cactagcaat aattcgaacg gctgcaaaca
    36421 catcactatt tcttaatgca cgaacgcttg taaagctggt ggtgtaatca tccgattgga
    36481 ttgataccac atgttgtagg aaaggatctc ccgttgtatt ttgtctcgct ttaaaaaatg
    36541 ccatctttta acctcccttc tgttctgtat ttagcatgac cgctacccca ataagcacta
    36601 tgcccagcgc taacaagcca tagtagacat ttgttagtaa ggtgatagca accaacacac
    36661 acaccaatcc aagaagtaaa agaacggtat gaatgtaatt agaaagaaaa ttcttcactt
    36721 gtataaaatt cattgtctgc tttcgctcct tccgttgtgg tgaagtaatc cattgcaaac
    36781 acataggcat tgattaatgc agctattgga tcaattttgt tactgttctt tgatttatta
    36841 atctgaatgc cgttgttatc cgtttttaaa atagcgttgt tcactgcatg ggtgagaatc
    36901 gtatttttat tgtgaataac gttgccttca taaacttgtt ctctaaaagt tcgggtcgga
    36961 acgtttagag taatcgttcc ttgtcttact tctaacattg ggtagttggc tttttcagct
    37021 ttactgataa gcgaattagc gttatacggg tcataacaaa tccccataca ttccaaatca
    37081 ttttcttgaa tcaaatcttg aataaactga aatacttggt catagtccac gattccactt
    37141 tctaattgag taatggaaca ctcaccagct ttttctagtt ctcgataagg caaaccgtcg
    37201 cgcttttctt tgtcttgaag cccatatttg gtggctacaa agctatgtga atcacaataa
    37261 agttcgccgt tatctagcgg tacgatccat gaaacgctag ttaaatcatc tgttttagat
    37321 aaatccactc cgatataaac gggcttgcct gtaatatctt gtggttctac ttcggttgcc
    37381 tgccagtctt cagcaatcat atagctgtcc tcactcgctt gtctccataa attgaagttt
    37441 ttcaccaata cagaattaag gttattttgt ttcaaagcaa ggttcacatc atcttgaatg
    37501 gttggaatca ttactttttt gatttcttca ctttcaaaaa tcggattggc tttgatccat
    37561 ttctcttgat cgtgaatttc ttcgggatcg tccaattccc atatcgctat aaagtacctg
    37621 tcggcttgtt cttcgccttt tagcacacga tctaacaata agtattcctc gtacatagga
    37681 acgtttaaat caagcccagc ggtactaata acacacaata agccgttctt ttgttgggtc
    37741 attcctgact taatgacgtt gtacgtttta cgagtttttg cttcgtgcca ctcgtcaaga
    37801 attgcggttg ttggtgcaaa cccatctagc gtgctggttt cactagccag tgccatagcg
    37861 aacgaattag agggcaagtg ctgaatctta gagttcataa ttttcagttg tggtcttaaa
    37921 aacttactag acttgaccac gtttctaagt gaattagaca gcatatcata gcccaattta
    37981 gcttgtttca aagcgttact tacaaacaat acttgccttg cttctgctgg ttcttgttcc
    38041 ataatcaacg cattggcagc catgccactt gcgatatacg ttttcccatt cttacggctc
    38101 atactggtaa acgctcgatt gaatcgcctg taattccctt ctttagtacg ccagccataa
    38161 agactgccta gaatgaattt ttgaaatcct aacatcttga ttgctttacc gtccgtagat
    38221 ggtagcaact caatgaattt aatcgctttg gtggcttgtt cttcatcgaa cacataaggg
    38281 aagctatctg acttagaacg ctccaaatcg cttatatgac gctcacacgc ttggataatt
    38341 ttcttgggtg caatcagctt acctgatagg actttctcaa tgtaatcatt catgaattca
    38401 tcaactcttt gaacgggtct tcgggttcat catttttaac cttgttcaaa gctagttttg
    38461 atcgtgattc taaggtcata cctagtgagg tggcttgttt tttcaaatca ctcatagctt
    38521 gtgattgaac ccgaaccgct ggattggctt tctttactcc gttttcgcat gtgataaacg
    38581 tacctgattt tcttatctcg gcttcggctg ttttcattcg agcaaaggct aaacaatagg
    38641 ctaccaatga accataatct gcttcactca atggataatc tttttttaat gttggtacga
    38701 tacgattcca ttcacttcta gcgctcgctg ccatccagtc agggggcgtg atatttatta
    38761 atggttgctg cttgaacaat tcttctctag cgtccacacg gattgctttt tcttcacttg
    38821 atatattgcc ttttgtatcg tgtaatattt tagggtgtcc catgttgtcc tcctttcaga
    38881 caaaagaaaa aggaacgtaa gcaattaagc tcacattcct tcgtttttcg atcagaattt
    38941 ttctttatct aatttatcta tataaatgat ttttccgttc tgcatttgaa tggtcacttg
    39001 accaaaatga ggtaactcaa tggtgcgctg tttccctgtt ttatcagtaa taacaattgt
    39061 atttttcaat aaatctccta ctttcattat atactttttc acttgtgatt acaagtgtaa
    39121 tcgttatttg taattaccct tatgcctttt cacaacggtt atttgtacca aaatgagtag
    39181 gcatcgattg tcagtaagtt tcacactatg cccccttaac tcaaagggta ctcttttctc
    39241 gtctttgtct ttcattggct gtcttggaat tatggtgtgc atggcataaa gaacgaaaat
    39301 tagtttcatc taaccgtcta ttccaatcat cttttagttc aattttgtga tctacaacat
    39361 cagctttcct tatcaagcct tcttctaggc aatcctcaca tactggatga tttaaccgat
    39421 acaagtatga agcctttcgc catgttctac tttgatagaa ttgaaaatat ttaccttcgc
    39481 tttgctttct ttcttcacgc ttaattgccg tttgctttgc tttatgcttc ccacaatact
    39541 tctgcctata atcaatcaat gttttacatc cagcatgatt acattgtttt ttaactgcca
    39601 tagattacca accaaagcga tctcgttcgt cagaatcatc ttgttcccat tttgatttgt
    39661 attcaatata aggcgaaact atcttcaagc acgttccgtc tttaaaatta actttagtga
    39721 ttttatcttc ctcaaaacta ataaagtcta catcttcttg agtgtttgaa tactcaatgt
    39781 gtttctcacc atcgaagtat ctaatacttt ccatatgcat aattattcta cctccaataa
    39841 attagttatc aattcatcat ctgttagtag caaagccaag aacttatcat ggatgtttaa
    39901 aaacaaatta tcctcattcg atgtaagctc tcgttgtctc gctcgatcat ataggttaag
    39961 cagacaatta aattcatcca ttagctcacc tgtatcatca tagttgccac tatctaccaa
    40021 tgatcccact actagcgatt gacaccacgc tactgactcc tgcagccaat caggttcttc
    40081 ttccattatc tttaatactt ctggatcaaa tgtggaataa tcttctgtta ccaatcccac
    40141 actaaatgcc agcgtgttca ttagaaaccg atccatttca tctgtatatt gaaagttcgg
    40201 tgctttgttt gttgaaataa tattgagcat ttactttcct ccttgattcg taacacgtgg
    40261 taacacctac gtaacaggta cccgttacca ctcaatccca acggggacaa gggttctagc
    40321 gttttggtaa caggtaacgg gtaaaatcca gtaacattat tccccagcat attagctaac
    40381 tatttcaaaa gttttaagga aataaggtgt taccccgtta ccaaattgct gtatcccttg
    40441 gggcagtaag ggtaaactag taacgggcta ggtgttactg acccgttact acccgttact
    40501 ttttgtactt tccagctcga tttttatttt cataggcatt tgacttattg taaaatcctc
    40561 gtcgtttatt tctttttggc agttgcttcc gttcccaacc aggaatactg ctaataatca
    40621 aggcaatctt tttagtttct gcatctgatc gaccattcaa taaatccttt gcttgtttat
    40681 caaaaacagc ttccaaaata tcagcggtga gtacgctatc aactaaataa aactcttgtt
    40741 gcatagtagc tccattatag aaatgctgta aatttttctc attacattta ctgtcaggat
    40801 agttctgata ataagaacgt ctcgcccacg ctggagcttc ttcccattta tacgggattt
    40861 ccatttccaa atatttagtg attgcttctt taattggatt ctccatcttg gcatcctctt
    40921 gatagtcttt tgcaatttct aagacttctt tatcttcatc gggactaaaa tagattcgtt
    40981 gtcctttatc aaaaagcacc tttgcttcag ctaacacttg aagaaaatat tcatcctttg
    41041 ttttaaacgg attcatcttt ggcttattct tacaaggcaa aggataaaaa cggcggtttc
    41101 ctgtatcatc tttcaaatac tgcccatcat tcgacgttcc aataaataca cattgccttg
    41161 cccaagctat gacattccga ccataaggag gtctaatttt atcgatcttg gcactgatga
    41221 agttttttat ctgttcaatt ttagttttgt tcattgaaga taattctcca agttcaataa
    41281 ttacattgcc ttgaagctgc atataatcat ctttcttttc acctagcgaa tctaatgaat
    41341 cggtgaaaaa atcatccgtg taaagtaatg ctggaacggt actcttacca atcccttgcg
    41401 gtccatccaa aactggaacg atctcaaact ttattcctgg tttatagata cgtgcgactg
    41461 atccgactat ccaacgtttt gttacttctc gtgtgtaaag gttgtcgtct acccctaaaa
    41521 ggtcaataaa cagtgtttcc agtctaggct gttgatccca tttaactttt tcgatacgtt
    41581 cttttactgg gttgaaaaca ttctttctag ccacatttac tactgctttg tgtattttgt
    41641 tgtcatcaaa aactacattg tagttttctt caatatatgc cgttaaggca tcctcaaaac
    41701 tgtcttccca ttctccagcg gtacgattga tccaaggaga atcttttaat aggtgaatgt
    41761 atccgctaaa ttcgttataa gcaattgttc cagctaaatt gctatcattg gttaaaatca
    41821 attctgcatt cacaacactg ttacttttaa tctgatcgtc tttattgcat cttaatcgtg
    41881 acatccaggt tttcaattca atgatattgt cttcacgctc gctggcttcg ggaaagtcta
    41941 aaatactatc taatttatgc ttatgagcca tgagctttcc ccctcaaatc ttttttaatc
    42001 atcgatcgaa taatttgatt cacttcttta tcttttagcg gtggttcatt gtacgcattt
    42061 gctgtataaa tcaattctgc taaagtttga ttatctacac cgtgccatag taaccaaccg
    42121 cataacttag ttaaatgatc gtttctacta ccttcgccag cgccttgaac aatcatatct
    42181 aatttttttg ctgtccctgt ttttgctacg ggtcggtatt gagtctttat ttcttgcggt
    42241 ttttttagca gtaggttcac tagccaatcg ggagctggtg caatattatt tagagatacg
    42301 ttttcaatag gttcatatgc tctcccaaca cttcttgaaa taatcatagt tggcgctatt
    42361 ggaactcctt tagtgataat atcaatcccg cttaaacttt tacctttatc atcacgcccg
    42421 aagatatcag caaatgcacc tgtttcttgt tggataatta caccatcagg aattttaaag
    42481 aagaaatgga ttcctttacc tggtgtgatt tccgtataag tagaaggaaa tggatcatac
    42541 attgaagcaa gttttttata attctcaacg ccgtttcgtg tttcagaatg ttgatctagg
    42601 tcaactacca ccatttgatg atcttcaagc attaacccaa tatttctagt tgcatcttcc
    42661 gtccaccatt cattaatttg attagaatcg ttagttgcat cattccaacc actagagcct
    42721 tttattggaa ttttagttcc aggagataag ggataaattg accatcccga cttttgtaat
    42781 tccgttgctg cttgataatt atttgccaat aaacctctcc tcctttttca aaatactggt
    42841 ataaatcatt cagacacctc catgagcttc aactgctcaa gttcgtattt caacccatcc
    42901 cgaacaaatt cagcaccttt taattgattc tgatacttta cccatgtatt aaatgttttc
    42961 acccttgctt cttgcatatc agtaatgttt aaccatgcgg ttaaaatctg attttttagc
    43021 tcgttgattt ctcgacaaat tattttgtgt tctgcatgtt ttttgagtac atacaaaact
    43081 gttagttttt cattgaatga atttccaaag taggttttcc cgatattatt ttctagttca
    43141 gcgatccctt gattaatttc ctctaattca aggctatcga gttggtttaa tagttttgtt
    43201 ttgtacatct acttatgccc tccagttctt aattgatagt cgtactcgtc ataagcagtg
    43261 aagagatata acgctaatcc tgcaaagata gctatttttg caattaatgg taataagccg
    43321 aaaattccgc cgattaaaat gcttcctata attaagtttc gtttcataat ggttccctcc
    43381 gattttttaa tatggtataa tcggaacaaa gaatatcggg ttttatccca attaagttgc
    43441 ctttatcctg tccgccaaga ctgttatgga taaggctttt tctttgttct cgatcatgtt
    43501 aatgctccta agttgtctgc aattcagcag aacgattata aattttctca ctcactgaaa
    43561 actgcaaccc atatttttct ttaacagtca ttaattctac tgtctcatct aaaataggtt
    43621 cacgatcaac caacatttca ggagtcatat ctttcttttt taccattttg gaatgaccat
    43681 aaatagttga tacagcttta ttggctatca cattcgcttt aatgtagtct actggttttg
    43741 gttcagtaat agcgtggctt aatttagtca ttgcttcttt ttggtgttct ttgtctagca
    43801 tgcggaatac ttgaaagcct tcaagtcctg tggactggcg tagttctttg atgatgtcga
    43861 aaatccagtc ttgaaatgcc tcggcttctt ttttacggct tttgaatgcc atgcggtaaa
    43921 tatttttttt cgttgatgat gtttacatct tgtgttctgc ccaatgaatc gatgacctta
    43981 ctagtagtaa ccccatcttt aggcaaagag tgaatggctc tagtaacctg tttaagacct
    44041 aacgcttccg caacatcttt cgccactgcc caccattcat cattaatatc gacaaatctt
    44101 ataatgtgtc cgttccaaat ttcagttttc attcaacgtc ctccagttca agccctagca
    44161 agctcgctaa caactcaact tgctgataca aaatttcttg atactcttca atagaaagtc
    44221 gttctttaaa ctctgcttgt actaaatcgt tcaagccacg caatactccg cttaccgttg
    44281 gttcttgtgg tagcaatttt tgatagtcca tttgttcgta caaacattca aacgtttctt
    44341 ctagcgcttt gccttccaag tttgctaatt ctgccgctgt gtcttctgta agaattgttg
    44401 ataatacttt ttcgattgct ttattttgtg tcgattccat tattatttcc tcctattaat
    44461 ccctttgccc ccacggcttg cgtttatttt tggtattctt ccataaagcg atcacattca
    44521 tcttgtgaaa ttcttacgac tccatcaact ataatcaact ttaaaccgtt aggaacatat
    44581 ttctgcatca tagtattgta tgaaacgttc atatacttag cagcttgtgc cttgttcata
    44641 tattttggaa actggtactt tttttctaat aatgaaagta acgttccttg agtttgctct
    44701 acttgttgaa caactcgatc gtcaataatt ttaaaagctg gatcatcaag cgtgagtttg
    44761 tcaattttta attccatatt tatcccccct cgttagcata catttctata caaagatttt
    44821 aaaaaaatgc aagaggctct tgaatacatt tctatacctc ttataaactc aatataccat
    44881 catacctttt catttgcaag aattttaaca tacttttttg aacttttatg tttcacacat
    44941 atttaaatcg tgatatagta gagaaaatat atgaatggtg gtgaaaatgt ggctagaaaa
    45001 aaataccccg aagcagtgga gacaggacaa agaattagac tggctagaat tgaaaaggga
    45061 ctctcaatgg aacaacttgg aggtaaatta tccccaccgg ccagtaaagg tgccgttagt
    45121 aattgggaaa atggctataa tcttcccaat aacaccaggc ttaaacaact ttcaaaggta
    45181 ttagatgtat ccactaccta tctattaaac ggatcttata cattaagaga tatgcatatg
    45241 atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga aaaaaactat tgaaaaaaat
    45301 aactctataa ttgattctaa tctttctaat gctatcaata acatcgaaga agataacctg
    45361 actgtctcac agaagattta tttaacaaac gcaataaaat atcttaaaac aggatctcga
    45421 attgaatttc ctgacgggag taccccaatt gcttatctcg cagctcttct aaatacgctt
    45481 ttacgatatg actcattaat taatgattca gaaaaaaata cagatgagaa aaaagagtat
    45541 caagaatttt atgaaaatga tcttctaaaa gagttcacag ctcttgttac tgagctaaat
    45601 aaacactatt caaaataata ataaactgcc tcctccaacc gcccccacgg cttgctacac
    45661 tatggaggga aaactattat ggcaacattc aagcaatatg aaactaaaaa aggtaaattt
    45721 tggctctatg aagcctatct tggaatgaac aaaatgactg gcaaacctga taaggttcgt
    45781 agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct tgtcacgctt gcaagtggac
    45841 tacgataaaa acggtattaa aaaagcgaac aacgaaacgt ttaaagaagt ttatgacttg
    45901 tggctagaaa catataaaac cactgtccga gaagccacct tcatgaaaac tgaaatcaag
    45961 tttagaaagt ggattcttcc aaaatatgga aatcttcgtg tgaatgaagt cacggtaaaa
    46021 aaagcccaac aaattgtaaa tagctgggct gaaactactg accaatacaa ggtacttcat
    46081 tcaacttcta agcgtatttt tagctacgct atcaatcttg ggattattga gactaatcca
    46141 ctagacaacg tattaatgcc taaaagaacc accactaagc ataaagaaaa ggtcaaagtt
    46201 tacagtaaag aacaactgat caccttgttt gattacctaa acagcaaacc tgaaacttat
    46261 cgaaacgact ttgataaaac gttactacgc tttctctttt atagcggggt taggatcagt
    46321 gaagcccttg cattgaattg gtcagacatt gattttcaag aaaatacggt caccatcaat
    46381 aaaacactaa gccaatccaa aaatggctat aaaatcagtg atcctaaaac tgattgtagt
    46441 gctggggttc ttccactaga tgaaagaaca atttctatat taaaaaaatg gcagattaat
    46501 cagcggaaat atatgctgac acttggtgta actgatccga ctatgatttt ttgtggtatc
    46561 tataaagaac taatcacaca ccacgctatt tatgcaagat tgattactat ttgtgaaaaa
    46621 gctatggttc catttctcgg tgtgcatgta gcaagacaca ctcatgcttc aatgctcttg
    46681 gacgctggag ctactatggt agaagttcaa actagattac ggcatacaaa aattagtcag
    46741 acgatcgatt gttacggtca cttggcaaaa gaaacgaaag aaaagactgt tgaaaaatta
    46801 gtacaacact taaatattga ctagatggca tacaatatgg catacagtaa aaaataaaaa
    46861 aaggagaaac cctatcatat caaggtttct cctagcttta tagtctattc ttcttcttcc
    46921 gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt
    46981 tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca
    47041 tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc aaattcttct
    47101 tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc atccaccaat
    47161 gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat aagattacct
    47221 cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaagatc
    47281 atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt atttgcctgc
    47341 tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata atcaactggc
    47401 aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc ccttggtccg
    47461 atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc atattgtgct
    47521 actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat ccgaatgatc
    47581 tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac tgtcttagaa
    47641 ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg tagtccttga
    47701 caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga gcatcacgat
    47761 aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga ttgattggat
    47821 tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt
    47881 tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc
    47941 tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc gaagaaaggg
    48001 aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat ttgtatgttt
    48061 ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata cgctatattt
    48121 ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt cgaatagctg
    48181 ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg
    48241 aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa
    48301 ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac ggtagcgtac
    48361 atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg
    48421 agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag
    48481 ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta atttcttttg
    48541 tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga acaaatcttt
    48601 tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg aatagctttc
    48661 ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat tcccattgat
    48721 ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag aagcttgtgg
    48781 tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt gtgtggtgga
    48841 aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct tggttgtttt
    48901 tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata tgttccccta
    48961 taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt aagatggctt
    49021 aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct atctgtaagg
    49081 tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat ttcacaaccg
    49141 cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca agttaaaaac
    49201 ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc tttttcacaa
    49261 cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt
    49321 taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa
    49381 gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta atcaatcaat
    49441 tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact
    49501 cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag
    49561 tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct
    49621 ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat
    49681 taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct
    49741 aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc
    49801 tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg
    49861 cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta
    49921 tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt
    49981 gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat
    50041 cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga
    50101 tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc
    50161 tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac
    50221 aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc
    50281 cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg
    50341 ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct
    50401 tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt
    50461 aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc
    50521 ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat
    50581 tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat
    50641 ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa
    50701 ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca
    50761 aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta
    50821 ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg
    50881 agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc
    50941 acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat
    51001 agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa
    51061 aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc
    51121 atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc
    51181 cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag
    51241 ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt
    51301 ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac
    51361 ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct
    51421 tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat
    51481 gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt
    51541 tcgcctccta gggc
//
LOCUS       DARDHD010000005        51075 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000005 DARDHD010000000
VERSION     DARDHD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.005"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..1124)
                     /locus_tag="SDA06_000233"
     CDS             complement(105..1124)
                     /locus_tag="SDA06_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4227532.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="SDA06_000234"
     CDS             complement(1146..2153)
                     /locus_tag="SDA06_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4227533.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="SDA06_000235"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="SDA06_000235"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4227534.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="SDA06_000236"
     CDS             complement(2804..5098)
                     /locus_tag="SDA06_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4227535.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="SDA06_000237"
     CDS             5358..7523
                     /locus_tag="SDA06_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4227536.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="SDA06_000238"
     CDS             7827..8660
                     /locus_tag="SDA06_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG4227537.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="SDA06_000239"
     CDS             complement(8762..10462)
                     /locus_tag="SDA06_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227538.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="SDA06_000240"
     CDS             10969..12129
                     /locus_tag="SDA06_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4227539.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="SDA06_000241"
     CDS             12145..12588
                     /locus_tag="SDA06_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4227540.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="SDA06_000242"
     CDS             complement(13071..13601)
                     /locus_tag="SDA06_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG4227541.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="SDA06_000243"
     CDS             13706..14257
                     /locus_tag="SDA06_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4227542.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="SDA06_000244"
     CDS             complement(14410..14883)
                     /locus_tag="SDA06_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4227543.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="SDA06_000245"
     CDS             15021..16493
                     /locus_tag="SDA06_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4227544.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="SDA06_000246"
     CDS             16490..17200
                     /locus_tag="SDA06_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG4227545.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17552..17836
                     /locus_tag="SDA06_000247"
     CDS             17552..17836
                     /locus_tag="SDA06_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG4227546.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18117..18536)
                     /locus_tag="SDA06_000248"
     CDS             complement(18117..18536)
                     /locus_tag="SDA06_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG4227547.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(18538..19122)
                     /locus_tag="SDA06_000249"
     CDS             complement(18538..19122)
                     /locus_tag="SDA06_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG4227548.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(19169..20644)
                     /locus_tag="SDA06_000250"
     CDS             complement(19169..20644)
                     /locus_tag="SDA06_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG4227549.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20825..21136
                     /locus_tag="SDA06_000251"
     CDS             20825..21136
                     /locus_tag="SDA06_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227550.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21251..23587)
                     /locus_tag="SDA06_000252"
     CDS             complement(21251..23587)
                     /locus_tag="SDA06_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227551.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23729..24397
                     /locus_tag="SDA06_000253"
     CDS             23729..24397
                     /locus_tag="SDA06_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4227552.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24400..25443
                     /locus_tag="SDA06_000254"
     CDS             24400..25443
                     /locus_tag="SDA06_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4227553.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25680..26561)
                     /locus_tag="SDA06_000255"
     CDS             complement(25680..26561)
                     /locus_tag="SDA06_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4227554.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26720..27448
                     /locus_tag="SDA06_000256"
     CDS             26720..27448
                     /locus_tag="SDA06_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG4227555.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27604..27921
                     /locus_tag="SDA06_000257"
     CDS             27604..27921
                     /locus_tag="SDA06_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4227556.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27914..28522
                     /locus_tag="SDA06_000258"
     CDS             27914..28522
                     /locus_tag="SDA06_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG4227557.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28577..29368
                     /locus_tag="SDA06_000259"
     CDS             28577..29368
                     /locus_tag="SDA06_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG4227558.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29506..30018
                     /locus_tag="SDA06_000260"
     CDS             29506..30018
                     /locus_tag="SDA06_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG4227559.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30044..30916
                     /locus_tag="SDA06_000261"
     CDS             30044..30916
                     /locus_tag="SDA06_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4227560.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31092..31454
                     /locus_tag="SDA06_000262"
     CDS             31092..31454
                     /locus_tag="SDA06_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4227561.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31463..32215
                     /locus_tag="SDA06_000263"
     CDS             31463..32215
                     /locus_tag="SDA06_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227562.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32229..34031
                     /locus_tag="SDA06_000264"
     CDS             32229..34031
                     /locus_tag="SDA06_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227563.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34308..35348
                     /locus_tag="SDA06_000265"
     CDS             34308..35348
                     /locus_tag="SDA06_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4227564.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(35400..37544)
                     /locus_tag="SDA06_000266"
     CDS             complement(35400..37544)
                     /locus_tag="SDA06_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4227565.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37901..39274)
                     /locus_tag="SDA06_000267"
     CDS             complement(37901..39274)
                     /locus_tag="SDA06_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4227566.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="SDA06_000268"
     CDS             complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="SDA06_000268"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG4227567.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="SDA06_000269"
     CDS             complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="SDA06_000269"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG4227568.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40722..41735)
                     /locus_tag="SDA06_000270"
     CDS             complement(40722..41735)
                     /locus_tag="SDA06_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4227569.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41816..42415)
                     /locus_tag="SDA06_000271"
     CDS             complement(41816..42415)
                     /locus_tag="SDA06_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4227570.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42601..43302
                     /locus_tag="SDA06_000272"
     CDS             42601..43302
                     /locus_tag="SDA06_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227571.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43312..46023
                     /locus_tag="SDA06_000273"
     CDS             43312..46023
                     /locus_tag="SDA06_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227572.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46420..48048
                     /gene="treC"
                     /locus_tag="SDA06_000274"
     CDS             46420..48048
                     /gene="treC"
                     /locus_tag="SDA06_000274"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG4227573.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48108..49289)
                     /locus_tag="SDA06_000275"
     CDS             complement(48108..49289)
                     /locus_tag="SDA06_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4227574.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49541..51064
                     /locus_tag="SDA06_000276"
     CDS             49541..51064
                     /locus_tag="SDA06_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4227575.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        16083 a         9170 c        10426 g        15396 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccgt acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagga
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 acacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg aaaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt caggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ctcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tccttttttc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctgttttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaacaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata caccaaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgct
    15361 tgatccaagc ttgcggcgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgtagg
    17221 aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca atcagataaa aatagcactc
    17281 catatttttc atctgattgg gcttagtgcc gtagatgcaa gcttttgaac accgtttaag
    17341 gaagaggttg tgagcctgcc gtttaactct gagtcacaag gagcaatttc aaaaaacagc
    17401 ttctcatgtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    17461 attcgaaaaa aggaactgcg acaagacttt tgtcacagtt ccttcttttt tttgctatgc
    17521 tatactagat gtaaaaaata ggagaaataa aatgagggaa atttattctt ttgaatcaaa
    17581 aatctatgct tccgaagtcg acaagggcgg ggcatatatt atttttccgt atgatataag
    17641 aaaagaattc ggtgctggaa gagttaaagt ccaagcaact tttgacaata tgccgtatga
    17701 aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt tcagtctgct acatccttgg
    17761 actacgaaaa gatattcgta aaaacatcgg aaaagaaatc ggtgatattg tagcagttac
    17821 agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt cacaacctct tgataaacgg
    17881 tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaacccatg
    17941 tttacaaaat ttttcttact actcgaaacg aagcggcttt atcacaacct cttgataaac
    18001 ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg gcaaaatatg agaagctatt
    18061 tttccatttt tttctctcat actcaggtca taacgctgat ttcacaacct cttctttcat
    18121 aatgtttttt caccatcttt ataataaact ggttctttga tatggaaatc tattttttcc
    18181 aaccaatcta aaatcttgta acctaactct tctgacatcg gaaaagaggc gttgatttgt
    18241 gcattggaga tatggtcaac atggataccg gaagtgatcg tacaacttcc cggaacggca
    18301 gttttgatga tcacagctat tttttcggct aagacatcgt ctttatggaa ccgaccatcg
    18361 tggctaggga aacgtacagt ttgcatctct gtatcacgag aaaatgtagt gactgttccg
    18421 atatgtggtg tatctccgcc tgttaaagtg atcaataagt ctttaccgat tactttaaca
    18481 gcagcttgga tcgtgaaacc tagtttggtt acttcaacta tcgtttcttt ttccatatta
    18541 atcaccctgc caacgttttg aatgctcatt tacaattcca aattgatcca aaagtttcat
    18601 tgtatggtga ttaacaagat cttggatcgt ttgaggctga ttataaaaag caggaattgg
    18661 tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag ttttccaagt gaatggcatt
    18721 taatggcgtt tcacgaggaa caatgactaa ctttcgttgc tccttcattg ttacatcagc
    18781 agcacgtgca atcaggttgt cgcctaaacc aacagagata cctgcgactg ttttcatact
    18841 tgccggtacg atgatcattc catcggtcaa aaatgatccg ctggcgacag cagcacctaa
    18901 atctttgttt gaataatggt aatcaaacag gctttcgaat tcagttagtg ataagtcgga
    18961 ttccagcttt aagttctgtt ttgcccatgg actgatgatt ccgtgtgttt ctatcatcgg
    19021 atattctttt agtttcttca ctaggttgat ggcataaatg gtgcctgaag cgccacttac
    19081 accaatcacg atttttttct tccgttgtgc caattgttcc attttccgca ctccttttta
    19141 tgatgtcaaa acagttgcag atcattcatt atttcaaata tttttcccaa tcttctactt
    19201 cttggaattt tgcacgtaca aaatgttctt tcatatcgaa aggaactgta ccgtcaaaga
    19261 tagtttttgc actcatccca cggaaacgaa tagagccagg attatattct ggtaattctg
    19321 atgggtccaa tggatggttc ctcattcctg ggatcacgat gatatcttta tctccttgga
    19381 aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa aatatcaaca tcttcatcga
    19441 ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc aagaatagct gcttgacgct
    19501 gaataccttc atccgcttcg ttttctttac ggatttgcat gatggtcatc aatttaccac
    19561 cacctgcagg aggattgtag acatttacta cttttcctgg aatagcttta tcgaccaagg
    19621 caagaatact tgcttctgta ggaataccgg ccatagatac gtgttcttca ctgggaccaa
    19681 tcgttgtttg catgattgga ttgtcttttc ggtgagtaac tgctttgact ttcactactt
    19741 gtacagctgg attagcatcc ccagtatagc caggaaattc tggcattgct tttcctgagt
    19801 tcgtgttgat atcttcttgc attgtttcat ttggcatgat ttctgcttca atgatgaatt
    19861 cagcacgagc aatacccact tcatcgactg ctataccatc aacgatttga ataggttcgt
    19921 tacgcaaagc tcctgcacac catagttcat tgtagccaag tggagtggta ggtggttcga
    19981 aagtcgtgcc aatggcaatt gctggatcta aaccaatatt gatagtgata ggcattggtt
    20041 tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg gcgtccgcca ggcatgatgt
    20101 acataccgat cgtatcttta tcttctagaa ccatacgatg gatcgttaca tcgctcattg
    20161 ttttttctgg atctgatcct aagactacac ccattgtgat gtatggacca gcatcttcag
    20221 gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa gtctgcatcg gtagctagat
    20281 gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt cacaggtttg attggatttt
    20341 ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc atggtgcaaa atttcaccta
    20401 cacgtttacg actagccatt gttccgatgt tcacacgtgt gccggggaaa cctttgatat
    20461 tgttgaaagt catagctggt ccttcttgag tcggacgttt tactgtaccg ccagcaccga
    20521 tataacggta aacaccagat aattctgctt caggatcgat ttcaatatct gtttcgtgat
    20581 attgacctgg aagtgttttt agttcttcga tgacttttct taaatcatac ggttgatctg
    20641 tcatgagaga ttcctcctta gttgtcttta cctatctata gtaaaaggtg aaaaagagga
    20701 aaacaattca acttgttcgg taaagtattc tacaatggaa tggatgcgtt tttttgataa
    20761 tagaataaac tatgatatac ttatcactaa aagaaagcgc atacttagtg ttggaggaag
    20821 gaaagtgaaa aaaatgaagg agtcatttcc taaagcagta aaagtggaaa atatagcgaa
    20881 tattttgaaa gttacatttg aaaacggaga agtaaaatac gtgaaatctc attggacaga
    20941 agagataacc gatgccttgc aatttggaaa aaaaggaaga ggaaaaagaa aaaatcttct
    21001 cgcactttct cgtaatatgt ggattgggac tgaagtaacg attgaagcag atggaacagt
    21061 attcataaat ggaaaagaca ggtatacgcc agaagagttg tggtacaaag gaaagaaaag
    21121 tataccagaa ttataaaaaa taattaaaaa aaacagcgcg aaacaaggaa catattactt
    21181 cctcgtttct tgctgttttc tactatttca gcgctcttat gactcaaatc taaatgtggt
    21241 tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc ttttttcgct gccattcgtg
    21301 caggaagatg accgccaagc atcgtcaata tagtagagac gatcactaga ataatcgcat
    21361 ggacaggatt tagttgagca acatttttta gatccgtcat gttgtataag atgctattga
    21421 tagggaacgt agctagccat gcaataaaca cacctaatat cccagaagaa atacctaaaa
    21481 tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct aaagctttta
    21541 gtacaccgat ttcttttgtc cgttcaatca cagatgtata agtaatgata ctgatcatga
    21601 tcatacttgt tactaaagag atccctgcaa aagcaataag gacataagtg atagcatcca
    21661 tcaatccgcc agtcagttca gtcatcgttc cagctaaatc agtataaatg attttatctt
    21721 catctgattt gcctttattg taatcatcta gataatccaa gatcttttct ttgtcttcaa
    21781 aattgttcgg ataaatcatg atacttgaag gcaagctgtc tccacctaaa taactaagca
    21841 atgtttgttt ggcattttcg tcaacttttt cagtagttaa gacatttaca ccactgtctc
    21901 tttgagcttt gacgatttct gattctttgt tttcgttgac gatttgagtc gttaattgat
    21961 cgctataagc gattcctgga gataagagat tcattgtaga agaagatttc acgcgtagga
    22021 tccccgagat cttcaattca tcagaagcat tctgatacat cgcatcataa tctgtattag
    22081 ggataaaatt cccagttgga agttttgtat agtaagtatt gttgtttact aatttgaatg
    22141 ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc tttcacatca aatccaagat
    22201 tcttcaatgc gttgatatta gtattattat tgccgtctac gatcagcacg acatctgttg
    22261 ctgaggctgg ataacttccg gcaagtaaag aataattgtc ttttaaaaag ttttctttgc
    22321 tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct gacatcgtac
    22381 tagataaaga aagagattcc gtatcaggat tttgattcga gaagctgaca ggctgtactt
    22441 tgccatttac atcacgtaaa agattgattc ctgtagtacg ggtaaatccg atattattac
    22501 ttaagttagg atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc ttattcgtgt
    22561 gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt tttgctgtct ggataatccg
    22621 ctttgcttgc acccaatccg gcatcgtcac gagtctggtt agtcgttacc ttagaaatag
    22681 taatcggaaa tttcgccatc gtttcagctt gcgtgttatc gatttgtttt tggaatccag
    22741 aagaaaggga cagaacgatc gcgattccga tgatcccaat actggaagca aaggaggtca
    22801 agaaagtacg tccttttttt gttcggatat tgttgaagga aagctttaac gctgtccaaa
    22861 aactcatctt tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg atgtgaggat
    22921 gggaatctgt taagatctta ccatcagaaa attcaatgat ccgatctgcg tattgatggg
    22981 ccagttcagg attatgtgtg accatgatca ctaatttttc tttcgataac tcttggatca
    23041 attccatgat ctggatactt gtttctgtat ctaacgctcc ggttggttca tcgcacaaaa
    23101 ggatatctgg gtcattggct agtgctcgag cgatggccac tcgctgcatt tgtccaccag
    23161 ataactgatt tggcttttta tgcatatgat ctgtcaagcc gactcggtgc aatgcatctt
    23221 ctgcccgttt tcgtttttcg tctttagaaa caccacttaa tgtcatccca agttcaacgt
    23281 tttcaataat gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa ccgatagagt
    23341 tgtttcggta ggcatcccag tcactgtcct taaagtcttt agtagacttt ccgttgatta
    23401 ccatatcacc ggaatcataa ttatctagtc caccaatcac attcagcata gtcgttttac
    23461 cagaaccact aggacctaaa atcgctacga attctttttg tctgaaagcg acagacacac
    23521 catccaatgc tttggtggtg gtttcgccga ctttataata tttttttata tcttttaatt
    23581 gaagcataat cacccttctc ccgtattgaa atattcggta tactaataag tatatcagaa
    23641 caatgatcgt agtatacgaa ataaatgtta actgaagatg aactacaaga aaaaatctag
    23701 aaatttttca aaaaacagag ggagatcaat gaatcgtatt ttagtagtag aagatgatcc
    23761 gcaattacag ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag tcatcaccgc
    23821 attgaatgga gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat taatcattac
    23881 agatatcatg atgccgaaaa tggatggcta tgacttgcta gactcattac gtagcaccag
    23941 atttgacaca cctgttctaa tgattacggc aaaggccgat tttgaggata agaaacgcgg
    24001 gttcaaatta ggagcagatg attatatgac aaaacccatc gatgtcaatg agatgatcct
    24061 acgtgtggaa gcattgcttc gacgttcaaa gatcaattac agcagtagct tgataatcgg
    24121 acaaacaaaa ttagagctag atgcttatac agtgttacaa gatcagcatt cgttgattct
    24181 gcctcaaaaa gaatttctgc ttctgtataa attactgtct tatcccaaca agatcttcac
    24241 tcgccaacag ttgatggatg atatttgggg attagatacg aatacagaag aacgaacgat
    24301 cgatgtacat atcaaacgtt taaggacccg ttttgaaaag acggaagatt ttcaaatcat
    24361 cacagtacgg ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa gctattttca
    24421 caattatgga tctattttgc tagtattgtc ttcatttcga tcttagtgac actgctgtgt
    24481 tttctcggtt ttattttctt attgtctcga cattctatct cgcctgctga atcggctgga
    24541 aaaccaacgt tatacccgtt attcgtcttt gcggggttca gtatggtcgt gggtacagga
    24601 atatcgattt ttgttgggcg gcgtatcctg catccaatca gtgcgctagg gacaaatatg
    24661 agtctggtag caacgggaga tttttcgatt cgaatggatg aacagcagaa agtagcagaa
    24721 gtacagcaac tgtataaaga tttcaacgtt atggtccaag aactcaatag tatcgagaca
    24781 ctgaggaatg actttgtgtc cagtgtatcc catgaattca aaacaccact agcaacaatc
    24841 caaggatatg ttcaattgtt acaagcccca aatctctctg acgaagaacg ccaaatattt
    24901 ttatatcgga ttatcgaaag tatcacgcag ctttcacaat taacagagaa catcttaaaa
    24961 ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg attagatgaa
    25021 caaatcagag aagtaatcgt ttttttgcag cctaaatggg agaaagaaca gctagaattg
    25081 gatatcgaac ttgctgcagt aaattacaca ggcaatgaag agtttttgta ccaagtgtgg
    25141 ctgaatatca tggataacgc cataaagtac aatcaaatca atggacagat tcatatcaaa
    25201 ctatttgaga cagccacaga gattggactg gaagtgaccg attcaggtgt aggaatgaat
    25261 gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt
    25321 tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt
    25381 attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa
    25441 taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt
    25501 ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt
    25561 attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga
    25621 gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct
    25681 tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg
    25741 actttacgag taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta
    25801 ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt
    25861 gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat
    25921 ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc
    25981 aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga
    26041 tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt
    26101 ggtggggcct ggaaatttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg
    26161 taagttaatt cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc
    26221 aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata
    26281 tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat
    26341 tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt
    26401 tttctatggg cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt
    26461 ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt
    26521 tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt
    26581 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
    26641 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
    26701 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgct
    26761 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
    26821 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
    26881 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtc tgattgttca
    26941 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
    27001 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
    27061 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
    27121 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
    27181 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
    27241 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
    27301 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
    27361 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
    27421 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
    27481 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
    27541 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
    27601 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
    27661 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
    27721 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
    27781 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
    27841 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
    27901 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
    27961 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
    28021 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
    28081 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
    28141 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
    28201 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
    28261 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
    28321 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
    28381 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
    28441 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
    28501 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
    28561 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
    28621 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
    28681 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
    28741 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
    28801 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
    28861 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
    28921 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
    28981 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
    29041 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
    29101 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
    29161 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
    29221 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
    29281 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
    29341 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
    29401 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
    29461 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
    29521 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
    29581 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
    29641 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
    29701 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
    29761 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
    29821 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
    29881 ttggattatt taccgcaggc agccggaggg acacaggcaa ggggttggta catcactgaa
    29941 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaat taaaaaagta
    30001 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
    30061 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
    30121 gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt taagtatttt
    30181 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
    30241 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
    30301 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
    30361 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
    30421 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
    30481 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
    30541 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
    30601 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
    30661 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
    30721 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
    30781 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
    30841 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
    30901 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
    30961 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
    31021 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
    31081 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
    31141 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
    31201 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
    31261 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
    31321 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
    31381 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac
    31441 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
    31501 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
    31561 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga cacttttgaa
    31621 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
    31681 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
    31741 ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
    31801 tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
    31861 atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac aaaaacaacg
    31921 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
    31981 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
    32041 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
    32101 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
    32161 ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac agtaggggga
    32221 gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta agagactact
    32281 tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt gaatcaatgg
    32341 caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt atcattttcc
    32401 gattcggttc tgttttacta gggatcatca cattcgttta tattttatat gcaaattcct
    32461 ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga gcaaaaggca
    32521 gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca gctactttag
    32581 tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta gtggatcagt
    32641 tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg atcactgttt
    32701 tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa
    32761 aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag cagcataaat
    32821 tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt tactatatga
    32881 tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt acgatcatat
    32941 taggaactta tttcattttc cattctgtga tcattttctt tttatcatta ctaaagaaat
    33001 cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta aactttcgta
    33061 ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg gcactgggcg
    33121 ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa aacaattctg
    33181 gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc aaagaacttt
    33241 caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt tactataatg
    33301 caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga acaccgccga
    33361 agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta caagaggaac
    33421 ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc agtcaatcag
    33481 aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta aaagacttcc
    33541 aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac aatccatctt
    33601 tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat aatggtctat
    33661 tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc atgcttgcta
    33721 gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc
    33781 ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg
    33841 tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga ttacaaatgt
    33901 ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg atcttcttcg
    33961 tattgtattt catctactat gtactgacga catggttata tactggaatc gtattgaaaa
    34021 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagctc
    34081 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgtttg
    34141 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
    34201 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
    34261 tgtatactgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
    34321 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
    34381 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
    34441 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
    34501 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
    34561 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
    34621 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
    34681 tcattttcta tcagactagc ccagatatgg gaaacggatt tcctggaact ttagatgtag
    34741 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
    34801 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
    34861 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
    34921 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
    34981 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
    35041 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
    35101 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
    35161 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
    35221 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
    35281 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
    35341 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
    35401 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
    35461 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
    35521 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
    35581 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
    35641 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
    35701 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
    35761 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
    35821 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
    35881 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
    35941 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
    36001 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
    36061 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
    36121 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
    36181 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
    36241 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
    36301 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
    36361 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
    36421 agatcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
    36481 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
    36541 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
    36601 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
    36661 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
    36721 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
    36781 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
    36841 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
    36901 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
    36961 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
    37021 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
    37081 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
    37141 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
    37201 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
    37261 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
    37321 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
    37381 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
    37441 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
    37501 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
    37561 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
    37621 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
    37681 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
    37741 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
    37801 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
    37861 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
    37921 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
    37981 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
    38041 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
    38101 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
    38161 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
    38221 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
    38281 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
    38341 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
    38401 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
    38461 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
    38521 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
    38581 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
    38641 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
    38701 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
    38761 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
    38821 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
    38881 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
    38941 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
    39001 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
    39061 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
    39121 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
    39181 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
    39241 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
    39301 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
    39361 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
    39421 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
    39481 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
    39541 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
    39601 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
    39661 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
    39721 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
    39781 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
    39841 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
    39901 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
    39961 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
    40021 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
    40081 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca ggatcgttgt
    40141 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
    40201 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
    40261 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
    40321 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
    40381 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
    40441 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
    40501 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
    40561 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
    40621 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
    40681 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
    40741 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
    40801 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
    40861 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
    40921 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
    40981 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
    41041 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
    41101 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
    41161 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
    41221 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
    41281 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
    41341 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
    41401 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
    41461 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
    41521 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
    41581 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
    41641 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
    41701 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
    41761 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
    41821 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
    41881 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
    41941 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
    42001 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
    42061 gagaagtacc aaaaaaagca cttggattgt attttttgat agtcaacata ttatctgtat
    42121 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
    42181 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
    42241 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
    42301 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
    42361 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
    42421 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
    42481 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
    42541 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
    42601 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
    42661 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
    42721 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
    42781 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
    42841 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
    42901 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
    42961 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
    43021 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
    43081 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
    43141 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
    43201 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
    43261 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
    43321 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
    43381 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
    43441 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
    43501 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
    43561 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
    43621 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
    43681 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
    43741 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
    43801 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
    43861 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
    43921 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
    43981 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
    44041 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
    44101 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
    44161 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
    44221 caaacattac ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
    44281 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
    44341 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
    44401 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
    44461 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
    44521 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
    44581 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
    44641 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
    44701 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
    44761 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
    44821 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
    44881 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
    44941 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
    45001 gattctctaa gttctgtttc agccaaacaa tttggaccga tcattgacta tcaagcaatc
    45061 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
    45121 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
    45181 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
    45241 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
    45301 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
    45361 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    45421 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    45481 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    45541 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    45601 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    45661 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    45721 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    45781 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    45841 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    45901 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    45961 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    46021 taaaaacaga ggttgtgaaa tcagcgtttt gagctaataa cgagggtcag ctgctaattg
    46081 aacaccatga atcagaggtt gtaaaaaagc cgtcctgcat cgagcaaaaa gaatactcaa
    46141 ataaaattag ctcctcatgt tttacatttg agaagactga ttgccgtaga taaaagcttt
    46201 tgaacaccat ttatagaaga aacaagaata ttttgctatt ttaaacaaga ggatagacaa
    46261 atacatgtct gatctcttgt tttttattgg gcaacagacc tcttagaatg tataaagata
    46321 aattttacaa acgctttctg ttcagttata ataataaatg ataacttgta tatataagct
    46381 gtggtaggta ataaatcagt tgaatggagg taaatatcca tgacatttaa agacaaagtc
    46441 atttaccaaa tctatcccaa atcttacaaa gacagtaatg gtgatggaat tggagattta
    46501 aaaggaatca aagaaaaact cccgtatctg aatgatctag gcatcgacat gatttggcta
    46561 aaccctattt atccaagccc tcaaaaagac aacggttatg atatctctga ttacacagcc
    46621 atcgatccta tctttggtac gatggaggaa ttcgaacagc taatagccga agcaaagaaa
    46681 taccggatag aaatcatgct cgatatggtg ctgaatcacg tttcgacaga acatgaatgg
    46741 ttcaaaaaag cactagcagg ggaccagcat tatcaggact tcttcatttt aagagaagag
    46801 ccaacggatt gggtatcaaa atttggtgga agtgcttggt cgccatttgg agatacgggg
    46861 aaatattatt tgcacttata tgatcggaca caagctgact tgaactggcg gaaccaagca
    46921 gtaagagagg aactattcaa agttgttcgt ttttggatgg aaaaaggggt aaaaggattc
    46981 cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat tgaaaaataa taaagaaaat
    47041 gatggcaaac ctgaatatac ggacaaacca atcacccata cgtatctaaa agaattgaac
    47101 caagcaacgt ttgggacgga ttccactatc atcacagtcg gtgaaatgag ctctacgacg
    47161 atcgaaaatt gtatcttgta tacagaacct agtagagaag aattatcaat ggtctttaat
    47221 ttccatcatt tgaaagtcga ttatgaaaat gggaataaat ggtctactcc tccatttgat
    47281 tttgcagaat taaagcgact atttcataca tggggagaaa atatgagtga gggtaacggc
    47341 tggaatgctc tattttttaa taatcatgat cagccacgtg cgctcaatcg atttgtagtt
    47401 atcgatcagt atcgaaaaca aggagcagag atgctagcaa ccatgatcca tttgaaccgt
    47461 gggacgccct atatttatat gggagaagaa atcggcatga tcgacccaga ctattcatcg
    47521 atttccgact atattgacgt agaaagtttg aatgcttata agttactcct tgaacaagga
    47581 ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat ctcgcgataa ctctcgaaca
    47641 cccatgcaat ggtcagacac agaacaagca ggatttacta cagggaaacc ttggttaaaa
    47701 gttggaggca agctagaaga gattaatgta gaaaaagaac tatcttctga agattctatt
    47761 ttgtcctatt acaagaaatt gattcggctg agaaaaacat atccaatcgt tgcacaagga
    47821 gattatcatg cttatgaagc aaatcatccg gaagtttatg gctttcttcg ccaatttgaa
    47881 ggacagcagc tgcttgtatt aacaaatttc tatgataaag atacaaggat cgccatccca
    47941 gaaggattca cagaagccaa tattcttatc gataattatt ccgatactgt tcttcatcaa
    48001 agtctggagc taaaaccata ccaggcaatt gctttgctga aaaattaaaa caaaaacaca
    48061 aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt ttgtgtttta tttaaaataa
    48121 tcttcttcag aaaatgaaga gttttcatac tctcctttat cgccataaaa tttattgtag
    48181 cgttgtttga atttacggaa aataaaagta acgagtaatt tgactaacgt atagatagga
    48241 ataccaaaaa taacgcccac aattcccatt agatcaccca tgacaagtaa aacgattaag
    48301 atcgtaatcg gatggatctg taaacgatca cccattactc gaggaatcac caagtcaccg
    48361 tggagaagtt gtacggcaaa ccagacaatg acaaatttga cagccataaa agtagagtct
    48421 tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg gcccaatata tggaatgata
    48481 cataacaacc cagcagaaat agctaaaaca ctagcaaatt ctaatccgat cagtaagaaa
    48541 caaacccagt agactgctcc aaggataaat gaagcaatga tttgcccctt tagaaatgaa
    48601 cccaattgtt gattagcaat ctttgtcaaa tttttaaaat ctgtacgaaa tgctggtggg
    48661 acaatcgcta aaactgactg ataaaatttt tgaggatctc tcaaaaggaa aaaagcgaca
    48721 attggaccag taaataaagt aataaaaata gctgtaacag ttgaaaaaat gttgctgatc
    48781 cgttgtgcgc cactttccca ataatttcca ataaatccag ctatatcaga cgtcacgtcg
    48841 tcaatagaag caaaaaattg attaaatgaa tcggcaaaag gtgttttctc caaaaattgt
    48901 cggagactct cttgaaaatc ttgaaacaaa tctggcaaac tactgatcaa tgtctttgcc
    48961 tgttcttcaa gaaaaggaat aagccatatg cttccaagcc ccaataaaaa taaaataatc
    49021 acataaacga taatgactga caagttcctc gaaaggtgct tgctcaaacg tttgatgact
    49081 ggttccaaaa gataatacaa gatcagtgca aacaataatg ggggcaagat agtagcgata
    49141 atgattccga caggcttgaa tataaacgag atctgatgga aaaaataaat gatcagcgca
    49201 agtaagataa ctattcctaa aacatagtaa gaggtttttc ctcctaaaaa gcggatcaaa
    49261 cgattcgttg tatctgtttc ttttttcact ctttttcctc ctttatctat aacttcatta
    49321 tagacaaaag cgggtataaa aggaaaatga tagaggttga ttgcaaaaat atcgtcaatc
    49381 attcatataa actttctcat caattaaaat aagaaaacgc ttgacagttc ttttaaccat
    49441 atgataaatt aaatcctgtt gctaaaacgt aactattacg gtttgcgaaa agcgaatcgg
    49501 aacattcaaa tgaaagaaga tgagaagagg agtgtcataa atgaaacgat tgataggtat
    49561 attggccatg ctggttttag caggtatgtt aactgcttgc ggggcaagtg ggaaagccga
    49621 ggacagtaaa gaaaaattga gcgtgatgac gaccttttat cccatgtatg attttacaaa
    49681 agcaatcgtc ggggatgaag gagaagtaga gttattgatt ccagcaggga ctgattccca
    49741 cgattatgaa ccttctgcta aagacatggc aaagatccaa gatacagata tctttgttta
    49801 taatgatgaa aacatggaaa catgggtccc cgcaatccaa aaaacattac aagaaggcaa
    49861 tgtccatacg ataaaagcga cagaaggtat gcttttactt ccgggaagtg aagaaggaca
    49921 tgatcatgac catgaacacg gggaagaagg ccatacacat gagctagacc cacatgtatg
    49981 gctagctcca agtttagcaa tcaagcaggt agcaaacata cgtgatcagc tgattgaagc
    50041 ttacccagaa aaacaggaag tatggacaaa aaatgcagca gcctataccg agaaacttca
    50101 agcactccat cagctttatc aagaaacctt taagcaagca aaacaaagat cttttgtcac
    50161 gcaacatgct gcattcaact atctagcgtt agaatatgga ttgaatcaag tatcgattgc
    50221 tggtctttct tctagtgaag aaccgtctgc ggcgagaatt gctgaactga aacactttgt
    50281 taaagaacat ggaatcaact atatttattt tgaagaaaac gccaaagata gtattgccag
    50341 aacgttagca aacgaagcag gtgtatcttt agaagtattg aatcctttgg aaggattgac
    50401 gaatgaacaa atagaaaatg gagaaaacta tctttctatc atggaagcaa acctcgaagc
    50461 gttgaaaaaa acgacagaga cagaaaaccc gctagaagat actttgaccc ccaaaaaaga
    50521 aaagagcgtc tacaacggtt attttgaaga cgaggatgta caagatcgct ctctttcaga
    50581 ttgggcagga acttggcaaa cagtagatac gtatgtagaa gatgggactt ttgatcctgt
    50641 ttttgaatat aaagaaaaat tgaatcagga taagacagca caagaatata aagaatatta
    50701 tacgaagggc tatcaaacag atattgcttc aatcaagatc agtgagaaag agatggcatt
    50761 tacgtttaag gatggaacaa cgaaacgttc aaattatcgt tatgtaggga aagaaatctt
    50821 gacctacgag gcaggaaacc gtggcgtaag gtatctattc gaagcagaag ataaggaaag
    50881 tggcgctttt agatatattc aattcagtga tcacacgatt gcaccggctg actccgaaca
    50941 ttttcattta tatgcaggaa atgaaagtca agaagtattg ctgaaagaaa tggagaattg
    51001 gccaacattt tatccagata caatggatgg acgtgaaatt gcacaggaaa tgcttttgca
    51061 ctgataggca aaaaa
//
LOCUS       DARDHD010000006        43639 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000006 DARDHD010000000
VERSION     DARDHD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.006"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..453)
                     /locus_tag="SDA06_000277"
     CDS             complement(109..453)
                     /locus_tag="SDA06_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4227576.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(446..880)
                     /locus_tag="SDA06_000278"
     CDS             complement(446..880)
                     /locus_tag="SDA06_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG4227577.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1018..1755
                     /locus_tag="SDA06_000279"
     CDS             1018..1755
                     /locus_tag="SDA06_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227578.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1748..2959
                     /locus_tag="SDA06_000280"
     CDS             1748..2959
                     /locus_tag="SDA06_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227579.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3163..3945
                     /locus_tag="SDA06_000281"
     CDS             3163..3945
                     /locus_tag="SDA06_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG4227580.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4015..4677
                     /gene="trmB"
                     /locus_tag="SDA06_000282"
     CDS             4015..4677
                     /gene="trmB"
                     /locus_tag="SDA06_000282"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG4227581.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4775..6529)
                     /gene="ade"
                     /locus_tag="SDA06_000283"
     CDS             complement(4775..6529)
                     /gene="ade"
                     /locus_tag="SDA06_000283"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG4227582.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6661..7590
                     /gene="tadA"
                     /locus_tag="SDA06_000284"
     CDS             6661..7590
                     /gene="tadA"
                     /locus_tag="SDA06_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG4227583.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7547..8596
                     /locus_tag="SDA06_000285"
     CDS             7547..8596
                     /locus_tag="SDA06_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG4227584.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8593..8871
                     /locus_tag="SDA06_000286"
     CDS             8593..8871
                     /locus_tag="SDA06_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG4227585.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8846..9316
                     /locus_tag="SDA06_000287"
     CDS             8846..9316
                     /locus_tag="SDA06_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG4227586.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9333..9635
                     /locus_tag="SDA06_000288"
     CDS             9333..9635
                     /locus_tag="SDA06_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227587.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9652..10023
                     /locus_tag="SDA06_000289"
     CDS             9652..10023
                     /locus_tag="SDA06_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG4227588.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9995..10354
                     /locus_tag="SDA06_000290"
     CDS             9995..10354
                     /locus_tag="SDA06_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227589.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10526..11533
                     /locus_tag="SDA06_000291"
     CDS             10526..11533
                     /locus_tag="SDA06_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4227590.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11558..12742
                     /locus_tag="SDA06_000292"
     CDS             11558..12742
                     /locus_tag="SDA06_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG4227591.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13179..14762)
                     /locus_tag="SDA06_000293"
     CDS             complement(13179..14762)
                     /locus_tag="SDA06_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4227592.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15340..15483)
                     /locus_tag="SDA06_000294"
     CDS             complement(15340..15483)
                     /locus_tag="SDA06_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG4227593.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15705..16184)
                     /locus_tag="SDA06_000295"
     CDS             complement(15705..16184)
                     /locus_tag="SDA06_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4227594.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16257..16541)
                     /locus_tag="SDA06_000296"
     CDS             complement(16257..16541)
                     /locus_tag="SDA06_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227595.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16619..16813)
                     /gene="ssrS"
                     /locus_tag="SDA06_000297"
     ncRNA           complement(16619..16813)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SDA06_000297"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16885..18171)
                     /locus_tag="SDA06_000298"
     CDS             complement(16885..18171)
                     /locus_tag="SDA06_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG4227596.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18309..18980
                     /locus_tag="SDA06_000299"
     CDS             18309..18980
                     /locus_tag="SDA06_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG4227597.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18946..19434
                     /locus_tag="SDA06_000300"
     CDS             18946..19434
                     /locus_tag="SDA06_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG4227598.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19447..20394
                     /gene="prmA"
                     /locus_tag="SDA06_000301"
     CDS             19447..20394
                     /gene="prmA"
                     /locus_tag="SDA06_000301"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG4227599.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20395..21159
                     /locus_tag="SDA06_000302"
     CDS             20395..21159
                     /locus_tag="SDA06_000302"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG4227600.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21392..23605
                     /locus_tag="SDA06_000303"
     CDS             21392..23605
                     /locus_tag="SDA06_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG4227601.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23616..24062
                     /locus_tag="SDA06_000304"
     CDS             23616..24062
                     /locus_tag="SDA06_000304"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG4227602.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24233..25492
                     /locus_tag="SDA06_000305"
     CDS             24233..25492
                     /locus_tag="SDA06_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG4227603.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25581..26528)
                     /locus_tag="SDA06_000306"
     CDS             complement(25581..26528)
                     /locus_tag="SDA06_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4227604.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26922..28214
                     /locus_tag="SDA06_000307"
     CDS             26922..28214
                     /locus_tag="SDA06_000307"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG4227605.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28229..29998
                     /gene="aspS"
                     /locus_tag="SDA06_000308"
     CDS             28229..29998
                     /gene="aspS"
                     /locus_tag="SDA06_000308"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG4227606.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30107..30982
                     /locus_tag="SDA06_000309"
     CDS             30107..30982
                     /locus_tag="SDA06_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4227607.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31248..33131
                     /locus_tag="SDA06_000310"
     CDS             31248..33131
                     /locus_tag="SDA06_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4227608.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33206..33490)
                     /locus_tag="SDA06_000311"
     CDS             complement(33206..33490)
                     /locus_tag="SDA06_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4227609.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33480..34214)
                     /locus_tag="SDA06_000312"
     CDS             complement(33480..34214)
                     /locus_tag="SDA06_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG4227610.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34343..34966
                     /locus_tag="SDA06_000313"
     CDS             34343..34966
                     /locus_tag="SDA06_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG4227611.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35158..36279
                     /locus_tag="SDA06_000314"
     CDS             35158..36279
                     /locus_tag="SDA06_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG4227612.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36286..39414
                     /locus_tag="SDA06_000315"
     CDS             36286..39414
                     /locus_tag="SDA06_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG4227613.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39433..42627
                     /locus_tag="SDA06_000316"
     CDS             39433..42627
                     /locus_tag="SDA06_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG4227614.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42773..43480)
                     /locus_tag="SDA06_000317"
     CDS             complement(42773..43480)
                     /locus_tag="SDA06_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4227615.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14075 a         7754 c         9218 g        12592 t
ORIGIN      
        1 acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta ggaaatacgc
       61 aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt atatttgttc
      121 aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt gtgtgatacg
      181 tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt ggaaagcagg
      241 aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat gattcaggct
      301 gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca gtttctttct
      361 tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag cagcagctcc
      421 aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt acgattgttt
      481 cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca aagtgaatag
      541 aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga aaaacagatt
      601 gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct ggaaaagctt
      661 tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca agttcgggat
      721 catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt cctttcgttg
      781 cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta tagcttggga
      841 tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc ctttctataa
      901 agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg aaaaaaacaa
      961 aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg aaaaagaatg
     1021 agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt aaaagatatc
     1081 agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg agcagggaaa
     1141 agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac gatagaaatc
     1201 aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg gtatattcca
     1261 gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt cactgcgatg
     1321 gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt ggagactttt
     1381 cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat gaaacagaaa
     1441 gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga cgaaccattt
     1501 ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga aatgaaaaaa
     1561 gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa atattgtgat
     1621 cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga gatgttgaga
     1681 aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt aacaaaggag
     1741 caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga aaatgatgaa
     1801 atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct tgattggcgg
     1861 tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg caattggagg
     1921 gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg ctacttttgc
     1981 agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa cctatttgga
     2041 acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg catgcggatt
     2101 ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt tcgtctatct
     2161 gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag aattgtttcg
     2221 actagatcaa caagttattc gttattggaa attaggctgg tttttttctt ctctgatctt
     2281 tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat tagtacaggc
     2341 aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt gggagaaaat
     2401 ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt tattcacaga
     2461 tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta tattgactca
     2521 tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat tggcaagagg
     2581 ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat tgattgccgg
     2641 agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt tgatcggttt
     2701 ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc tttatccaat
     2761 ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat taattatcgc
     2821 gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc gatgggttcc
     2881 gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac caactcgttt
     2941 gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat gaaaaataga
     3001 gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg ttttttgaat
     3061 ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg cgactagaat
     3121 aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga gtttcagtta
     3181 gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata cataggcatg
     3241 cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc tgctctttca
     3301 agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg cgatactttg
     3361 acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg tcaaagaaac
     3421 gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac catgctgacc
     3481 cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga aaaagaatta
     3541 ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct tcgtgagcag
     3601 atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa ccatcgtaat
     3661 tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat gtttgcagac
     3721 cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag ctggaatcag
     3781 tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt tcattggtat
     3841 gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga tcatcgacgg
     3901 atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt ggcccaaact
     3961 ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata aataatgcgt
     4021 gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt tgtaattagt
     4081 gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca tccaatccac
     4141 attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc ccatcctgaa
     4201 atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact agacaagttg
     4261 atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc gttgacagag
     4321 tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc atggccaaaa
     4381 aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga tgaacagatt
     4441 cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt tgagtattct
     4501 ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga tttacatcaa
     4561 agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc aaaaggtcaa
     4621 cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag tctgtgaaac
     4681 taactttctg ctttcataaa aacattaata tccataaata aatctcagaa aatgaacatt
     4741 acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt tcatcggttt
     4801 ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg tcggtgatct
     4861 tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga tcaaaggttt
     4921 tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca tagacgatcg
     4981 aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct atttcctgct
     5041 gtttgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca gcatacagca
     5101 tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca atcgttgtcg
     5161 ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg tttcccgttt
     5221 gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa ggaatgaatc
     5281 gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta ttcgggataa
     5341 tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt ggatttaatg
     5401 gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca gcaaataaat
     5461 ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact tcatgaattg
     5521 gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg gcaatcgcac
     5581 ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga atggcttgta
     5641 atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat ccttctttta
     5701 ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa ttttcaggtg
     5761 tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga accattaaat
     5821 acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca tgatccgtcc
     5881 gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct gcatgcccgt
     5941 caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct gtttcattat
     6001 ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt ttttgtaaaa
     6061 aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg gttacaggta
     6121 cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct tctatcataa
     6181 actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc actacagtag
     6241 taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt gagctttcga
     6301 tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca ttatgtacgc
     6361 tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct acatcttttt
     6421 ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact aggtcagcag
     6481 gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat ggagtgcctc
     6541 ccaagataaa tttttataag tataacataa attcggcttt tgccaatatt tttttgtaaa
     6601 ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga ggggagaata
     6661 atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa acatgcccaa
     6721 gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta tggaaaaaaa
     6781 cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc aagatttaag
     6841 tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc atttacttat
     6901 tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta ccttcaacga
     6961 gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg ttgctttagt
     7021 tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata ccttttcagc
     7081 gggacagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga agaaaaattg
     7141 caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct gcagctacag
     7201 atcaatccga aaatagcaca gacttatgat acacttttga aactcgcatt acggcatcgt
     7261 ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc tgcggtaaga
     7321 gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct tgaaggtaca
     7381 ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg tttagctggt
     7441 gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt atggagttat
     7501 caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga agaagctcag
     7561 gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc aaaaccagtt
     7621 tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat ccctttattt
     7681 attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac atcagctaaa
     7741 ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag agcaactggt
     7801 acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg aaattgccaa
     7861 acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg catatccgat
     7921 gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg ttttgcctca
     7981 gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga tacaagtctt
     8041 tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac tagtaaggta
     8101 tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac aattattgat
     8161 tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg gaaagctttt
     8221 tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga aacaagaaag
     8281 tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta tgtcattggc
     8341 tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg ttttacaggg
     8401 agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga caagaaaaca
     8461 gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt ttttgggcgt
     8521 agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg gaagcataga
     8581 ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac attgatcgaa
     8641 atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc taatttgtcg
     8701 cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt ggtagacaca
     8761 caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga ggaattgttg
     8821 aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg aaggtaaggt
     8881 tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac ttacaacgat
     8941 cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag tctatcaatt
     9001 tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg tgaatcagat
     9061 ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg ttccgaatcc
     9121 agataaaata aattgggaag tattagatat tcctgatgag attaccttga aacgccatgc
     9181 ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt atcaatttta
     9241 ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg gtcgctatac
     9301 aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg cttatgatcg
     9361 cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag gcagaaaaga
     9421 aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag acgcagcgga
     9481 tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc caaattgtcg
     9541 ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg atcagtattg
     9601 aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact gatgatattg
     9661 agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt aagtgaattt
     9721 ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga agaagaactc
     9781 aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat cgaggataaa
     9841 cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc cggcggacat
     9901 caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca cacaatttct
     9961 ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa aaacgatcag
    10021 tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc ttttatgtac
    10081 gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta atatacgaat
    10141 caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac agtctacgct
    10201 tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat atcagtgggt
    10261 acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt tcacagaaga
    10321 agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa acgccctctt
    10381 ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat aaaaaacagt
    10441 gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa aaattgttat
    10501 aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag ggtttaatca
    10561 aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt tagaagccta
    10621 tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg atggagtgcc
    10681 gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga aactttcttt
    10741 cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca gccagacaga
    10801 acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat ttgtttttct
    10861 tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata ttgcggcagg
    10921 aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt accaaacaga
    10981 aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta caagtgattt
    11041 gacacaagca aatgttcaat actttcatca agacgggttg caggaattat taatagatcc
    11101 tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg aaaaagcaaa
    11161 agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat tgctagagca
    11221 atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga gtggattttt
    11281 agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt atcttcaagg
    11341 aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa gtattttgat
    11401 cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg tgaaacttgc
    11461 ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa actggaaatc
    11521 atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa ttgcaatcaa
    11581 tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa ccgtagttgc
    11641 gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca agtacggcga
    11701 taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag taaaaatgct
    11761 gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca ccggagtagg
    11821 gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg atgagaaagt
    11881 aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg caaacttaat
    11941 ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg tattcgatac
    12001 atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg tgtcttatta
    12061 tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt atgtgtcgga
    12121 acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca cttgccactt
    12181 aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata cttctatggg
    12241 attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg atccagctgt
    12301 tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca atgttttgaa
    12361 caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg atcttgaaaa
    12421 gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc gcatccgaaa
    12481 atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct ttactgctgg
    12541 tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt cttggtttgg
    12601 ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata tctctacaaa
    12661 agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga ttgcccgtga
    12721 tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg aaacgcatgc
    12781 aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg taatttaact
    12841 tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa aagtggtcaa
    12901 aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata ttttattttc
    12961 attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta gatcagcctc
    13021 gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc cgaaacattc
    13081 attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc gtgaaagcac
    13141 aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag ttggccgagc
    13201 ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg ttgctgcaaa
    13261 aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag catttcttca
    13321 atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat caggacgttg
    13381 attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc ggataaatct
    13441 ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg ccgtcgagcg
    13501 acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc gggatcggca
    13561 aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt gatggtgcca
    13621 ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc tgcaccattg
    13681 tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc tttgaatagt
    13741 acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt gccagatttt
    13801 ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt gacgagtgtc
    13861 agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc attgtctcct
    13921 tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac ttctttttgc
    13981 tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac tcgaaaacgg
    14041 ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt ttttgtggtg
    14101 acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt tgtattctgt
    14161 aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg ttcatcgagt
    14221 aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac tcgttcgcct
    14281 ccgctaagtt ctgttaaccg tgcttcctga cagtgtggaa tccctaagcc ttgcaagatc
    14341 gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg ctgcagctca
    14401 ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa tgcttcgtag
    14461 cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa gtagtggttg
    14521 acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa gccttttttg
    14581 cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa aaaggttgat
    14641 ttgccagttc cgttgatatc gactaaggca attttttcac cagcttcaat ttgacaggat
    14701 aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc ttgtatgatc
    14761 atgatctctc tccttgagac cactcaaaaa ggccatggtt atccctggcc tgtgttattt
    14821 aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac gtgaattcaa
    14881 ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca tggctaaatg
    14941 cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc atcatagtat
    15001 tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga aagttcaact
    15061 agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc tttttttatc
    15121 tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac cagcgttcag
    15181 gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt tgccatttgc
    15241 ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat cgcgttcagg
    15301 agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga atgatttacc
    15361 gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta gtttggcgat
    15421 aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat cggaaacttc
    15481 cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac accagggcga
    15541 ctaatcgccc tggtgttcac tttttttatt ttacttgaac tcattgactg ttttcccttt
    15601 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    15661 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    15721 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    15781 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    15841 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    15901 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    15961 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    16021 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    16081 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    16141 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    16201 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    16261 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    16321 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    16381 attacgccta aaacagcata tgcagcaccg taaaattgta ggaatccttg atttttatca
    16441 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    16501 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    16561 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    16621 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    16681 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    16741 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    16801 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    16861 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    16921 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    16981 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    17041 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    17101 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    17161 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    17221 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    17281 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    17341 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    17401 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    17461 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    17521 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    17581 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    17641 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    17701 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    17761 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    17821 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    17881 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    17941 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    18001 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    18061 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    18121 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    18181 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    18241 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    18301 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    18361 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    18421 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    18481 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    18541 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    18601 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    18661 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    18721 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    18781 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    18841 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    18901 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    18961 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    19021 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    19081 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    19141 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    19201 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    19261 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    19321 gacgatgacg aagctgtatt cgagcttcac tgggataatc aacaactagc agaattagta
    19381 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    19441 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    19501 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    19561 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    19621 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    19681 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    19741 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    19801 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    19861 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    19921 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    19981 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    20041 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    20101 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    20161 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    20221 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    20281 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    20341 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    20401 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    20461 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    20521 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    20581 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    20641 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    20701 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    20761 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    20821 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    20881 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    20941 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    21001 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    21061 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    21121 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    21181 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    21241 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    21301 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    21361 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    21421 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    21481 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    21541 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    21601 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    21661 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    21721 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    21781 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    21841 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    21901 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    21961 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    22021 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    22081 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    22141 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    22201 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    22261 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    22321 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    22381 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    22441 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    22501 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    22561 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    22621 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    22681 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    22741 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    22801 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    22861 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    22921 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    22981 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    23041 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    23101 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    23161 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    23221 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    23281 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    23341 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    23401 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    23461 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    23521 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    23581 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    23641 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    23701 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    23761 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    23821 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    23881 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    23941 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    24001 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    24061 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    24121 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    24181 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    24241 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    24301 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    24361 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    24421 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    24481 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    24541 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    24601 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    24661 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    24721 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    24781 aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    24841 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    24901 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    24961 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    25021 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    25081 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    25141 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    25201 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    25261 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    25321 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    25381 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    25441 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    25501 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    25561 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    25621 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    25681 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    25741 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    25801 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    25861 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    25921 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    25981 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    26041 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    26101 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    26161 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    26221 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    26281 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    26341 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    26401 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    26461 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    26521 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    26581 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    26641 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    26701 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    26761 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    26821 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    26881 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    26941 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    27001 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    27061 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    27121 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    27181 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    27241 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    27301 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    27361 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    27421 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    27481 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    27541 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    27601 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    27661 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    27721 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    27781 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    27841 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    27901 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    27961 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    28021 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    28081 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    28141 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    28201 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    28261 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    28321 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    28381 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    28441 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    28501 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    28561 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    28621 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    28681 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    28741 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    28801 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    28861 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    28921 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    28981 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    29041 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    29101 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    29161 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    29221 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    29281 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    29341 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    29401 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    29461 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    29521 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    29581 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    29641 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    29701 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    29761 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    29821 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    29881 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    29941 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    30001 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    30061 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    30121 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    30181 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    30241 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    30301 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    30361 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    30421 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    30481 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    30541 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    30601 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    30661 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    30721 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    30781 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    30841 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    30901 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    30961 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    31021 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    31081 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    31141 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    31201 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    31261 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    31321 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    31381 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    31441 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    31501 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    31561 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    31621 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    31681 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    31741 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    31801 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    31861 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    31921 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    31981 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    32041 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    32101 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    32161 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    32221 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    32281 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    32341 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    32401 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    32461 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    32521 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    32581 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    32641 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    32701 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    32761 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    32821 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    32881 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    32941 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    33001 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    33061 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    33121 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    33181 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    33241 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    33301 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    33361 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    33421 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    33481 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    33541 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    33601 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    33661 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    33721 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    33781 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    33841 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    33901 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    33961 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    34021 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    34081 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    34141 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    34201 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    34261 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    34321 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    34381 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    34441 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    34501 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    34561 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    34621 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    34681 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    34741 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    34801 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    34861 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    34921 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    34981 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    35041 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    35101 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    35161 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    35221 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    35281 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    35341 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    35401 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    35461 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    35521 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    35581 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    35641 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    35701 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    35761 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    35821 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    35881 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    35941 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    36001 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    36061 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    36121 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    36181 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    36241 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    36301 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    36361 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    36421 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    36481 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    36541 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    36601 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    36661 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    36721 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    36781 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    36841 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    36901 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    36961 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    37021 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    37081 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    37141 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    37201 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    37261 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    37321 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    37381 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    37441 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    37501 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    37561 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    37621 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    37681 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    37741 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    37801 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    37861 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    37921 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    37981 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    38041 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    38101 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    38161 agacgaaaaa gcaagatggc caatgaaaga atagagctgg aagaaataag acaacaagca
    38221 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    38281 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    38341 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    38401 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    38461 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    38521 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    38581 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    38641 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    38701 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    38761 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    38821 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    38881 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    38941 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    39001 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    39061 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    39121 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    39181 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    39241 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    39301 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    39361 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    39421 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    39481 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    39541 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    39601 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    39661 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    39721 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    39781 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    39841 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    39901 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    39961 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    40021 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    40081 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    40141 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    40201 ataaatgaac aaaacgatca aggaatacaa ttttctgatc aaaacaaacc tttatcattc
    40261 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    40321 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    40381 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    40441 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    40501 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    40561 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    40621 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    40681 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    40741 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    40801 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    40861 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    40921 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    40981 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    41041 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    41101 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    41161 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    41221 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    41281 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    41341 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    41401 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    41461 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    41521 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    41581 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    41641 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    41701 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    41761 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    41821 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    41881 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    41941 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    42001 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    42061 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    42121 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    42181 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    42241 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    42301 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    42361 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    42421 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    42481 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    42541 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    42601 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    42661 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    42721 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    42781 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    42841 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    42901 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    42961 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    43021 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    43081 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    43141 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    43201 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    43261 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    43321 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    43381 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    43441 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    43501 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    43561 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    43621 tttcagtttt tttcactaa
//
LOCUS       DARDHD010000007        43557 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000007 DARDHD010000000
VERSION     DARDHD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.007"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(197..2578)
                     /locus_tag="SDA06_000318"
     CDS             complement(197..2578)
                     /locus_tag="SDA06_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4227616.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2568..3215)
                     /gene="recU"
                     /locus_tag="SDA06_000319"
     CDS             complement(2568..3215)
                     /gene="recU"
                     /locus_tag="SDA06_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG4227617.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3279..3818
                     /locus_tag="SDA06_000320"
     CDS             3279..3818
                     /locus_tag="SDA06_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG4227618.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3908..4327
                     /gene="gpsB"
                     /locus_tag="SDA06_000321"
     CDS             3908..4327
                     /gene="gpsB"
                     /locus_tag="SDA06_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG4227619.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4349..4716
                     /gene="rnpB"
                     /locus_tag="SDA06_000322"
     ncRNA           4349..4716
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SDA06_000322"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4925..6091
                     /locus_tag="SDA06_000323"
     CDS             4925..6091
                     /locus_tag="SDA06_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG4227620.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6137..7633
                     /locus_tag="SDA06_000324"
     CDS             6137..7633
                     /locus_tag="SDA06_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG4227621.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7714..8397
                     /locus_tag="SDA06_000325"
     CDS             7714..8397
                     /locus_tag="SDA06_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4227622.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9042..10232
                     /locus_tag="SDA06_000326"
     CDS             9042..10232
                     /locus_tag="SDA06_000326"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG4227623.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10334..11287
                     /locus_tag="SDA06_000327"
     CDS             10334..11287
                     /locus_tag="SDA06_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG4227624.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11356..12705
                     /locus_tag="SDA06_000328"
     CDS             11356..12705
                     /locus_tag="SDA06_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227625.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12888..13211)
                     /locus_tag="SDA06_000329"
     CDS             complement(12888..13211)
                     /locus_tag="SDA06_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227626.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13340..14503
                     /locus_tag="SDA06_000330"
     CDS             13340..14503
                     /locus_tag="SDA06_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4227627.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14573..15922
                     /locus_tag="SDA06_000331"
     CDS             14573..15922
                     /locus_tag="SDA06_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4227628.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15967..16398)
                     /locus_tag="SDA06_000332"
     CDS             complement(15967..16398)
                     /locus_tag="SDA06_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4227629.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16786..19428
                     /gene="alaS"
                     /locus_tag="SDA06_000333"
     CDS             16786..19428
                     /gene="alaS"
                     /locus_tag="SDA06_000333"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG4227630.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19628..20224
                     /locus_tag="SDA06_000334"
     CDS             19628..20224
                     /locus_tag="SDA06_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4227631.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20346..21494
                     /locus_tag="SDA06_000335"
     CDS             20346..21494
                     /locus_tag="SDA06_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4227632.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21532..21945)
                     /locus_tag="SDA06_000336"
     CDS             complement(21532..21945)
                     /locus_tag="SDA06_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG4227633.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22024..22455
                     /locus_tag="SDA06_000337"
     CDS             22024..22455
                     /locus_tag="SDA06_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG4227634.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22554..22787)
                     /locus_tag="SDA06_000338"
     CDS             complement(22554..22787)
                     /locus_tag="SDA06_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG4227635.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22970..24640
                     /locus_tag="SDA06_000339"
     CDS             22970..24640
                     /locus_tag="SDA06_000339"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG4227636.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24787..25173
                     /locus_tag="SDA06_000340"
     CDS             24787..25173
                     /locus_tag="SDA06_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227637.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25160..25858
                     /locus_tag="SDA06_000341"
     CDS             25160..25858
                     /locus_tag="SDA06_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4227638.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25863..26327
                     /locus_tag="SDA06_000342"
     CDS             25863..26327
                     /locus_tag="SDA06_000342"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG4227639.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26324..27235
                     /locus_tag="SDA06_000343"
     CDS             26324..27235
                     /locus_tag="SDA06_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4227640.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27470..28009
                     /gene="pyrR"
                     /locus_tag="SDA06_000344"
     CDS             27470..28009
                     /gene="pyrR"
                     /locus_tag="SDA06_000344"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG4227641.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28023..29306
                     /locus_tag="SDA06_000345"
     CDS             28023..29306
                     /locus_tag="SDA06_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG4227642.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29319..30245
                     /locus_tag="SDA06_000346"
     CDS             29319..30245
                     /locus_tag="SDA06_000346"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG4227643.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30264..31544
                     /locus_tag="SDA06_000347"
     CDS             30264..31544
                     /locus_tag="SDA06_000347"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG4227644.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31549..32625
                     /gene="carA"
                     /locus_tag="SDA06_000348"
     CDS             31549..32625
                     /gene="carA"
                     /locus_tag="SDA06_000348"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG4227645.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32630..35815
                     /gene="carB"
                     /locus_tag="SDA06_000349"
     CDS             32630..35815
                     /gene="carB"
                     /locus_tag="SDA06_000349"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG4227646.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35869..36645
                     /locus_tag="SDA06_000350"
     CDS             35869..36645
                     /locus_tag="SDA06_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG4227647.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36642..37574
                     /locus_tag="SDA06_000351"
     CDS             36642..37574
                     /locus_tag="SDA06_000351"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG4227648.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37571..38287
                     /gene="pyrF"
                     /locus_tag="SDA06_000352"
     CDS             37571..38287
                     /gene="pyrF"
                     /locus_tag="SDA06_000352"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG4227649.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38294..38983
                     /locus_tag="SDA06_000353"
     CDS             38294..38983
                     /locus_tag="SDA06_000353"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG4227650.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38956..39225
                     /locus_tag="SDA06_000354"
     CDS             38956..39225
                     /locus_tag="SDA06_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227651.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39263..39904
                     /locus_tag="SDA06_000355"
     CDS             39263..39904
                     /locus_tag="SDA06_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4227652.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39947..40639
                     /locus_tag="SDA06_000356"
     CDS             39947..40639
                     /locus_tag="SDA06_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG4227653.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40636..41538
                     /locus_tag="SDA06_000357"
     CDS             40636..41538
                     /locus_tag="SDA06_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4227654.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41696..43402)
                     /gene="efbA"
                     /locus_tag="SDA06_000358"
     CDS             complement(41696..43402)
                     /gene="efbA"
                     /locus_tag="SDA06_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG4227655.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13945 a         7684 c         9471 g        12457 t
ORIGIN      
        1 atggataaag tatatgtaat gataaaagaa aaatggattt tctaattttt aagctaaatg
       61 ttagatcaca catgtatatg ctggatagtc ttataaaaaa tcatgtaata cagcttggta
      121 catgattttt atttgtagct gttttttaca tagacacaaa aaagaaacca cgcttgtaga
      181 cgtggtttct tttttactat ctgtttccag aagctgaggt gctgctggat ggtgtatggt
      241 catttgcagc atccgctccg ctagttgctg gctcactgga agaagcagga ggagcagatg
      301 aagacggtgt cgagctttct tcggatgaag aagaaggaac cgttgttgaa gacgctggct
      361 cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc agttgattcg ctttgactag
      421 tagagctaga gctttgagtc gttgtctctg ttgttgagct tccaggagtc tggacatagc
      481 tggaagatgg aatagttgtt gatggtgtaa ttgcattcga tctagctgta tattgatctt
      541 tgtagtacag ttcaccgccc acacgaatta atccgcttgg catctcccag tctgtatttg
      601 tcacgttagc tgaaacatac tgcataagct cgcgataaac atcagaggct acgtgtgacg
      661 attctgaagt gacaggtgtc atttttttat tgtaacctgt ccacacggaa atcgaataat
      721 ttggcgtata gcccgcaaat aggatatctg gatacacccc agaagaaata ccaagttttg
      781 catactcgtc atctgtgtaa ttggatgttc cggtcttacc ggcttgatat aacccagcga
      841 tttgcgcatt cgtaccagtt ccttcagtga tcgtatcttt taaaatgtct gtgatcatat
      901 aagctgtttc tggagacatg gctgtttttc catctggctc ataagtttct tctgttccgt
      961 cttggaagac gattttatta acatattgag gtttgtaata agtccctcca ttggcgaaag
     1021 ccgcataggc tgctgccatt tttaaggaag aagctccgta ttttgtccca tcttgttctt
     1081 ccgtattact agagatcgca ttcgattgat ggatcgtact gtattcgatc ccaagatttt
     1141 ttaagaaaga agccacttta tctgaaccga cttcattgaa taatttgact gctggaacat
     1201 tacgggataa gtaaagtgct tggcgtaaag taatggtgcc catatattga ttatcccagt
     1261 ttcctacagg agtcgaagtc ccttcatagt tatacggtgc gtcagtaata gtttttccag
     1321 tagaatattt caaatattca aaagctggtc cgtaatctgt gacaggtttc atcgtagaac
     1381 caaaatcacg ggaagtattc actgctaagt tgttgcctaa agttacgtct tctgcgatgt
     1441 gacgtccgcc aatctgggct tttactttcc cggtattggt gtcgataagg gtggaggcaa
     1501 cttgcatctc gtcgtctggg taagagacat actgatccgt gttgacgata tcatatagtt
     1561 tcttttgggc gtccagatcc aaattcgtat aaatctctaa accgtctgtg tagacattct
     1621 tatctgtttt ttcttgtact tcattgatga cttctttgac ataattatcg acgatcttag
     1681 tattgtcatc ggattgagtt agttcttgca gaccatccgt taccggtaca ttaacagctt
     1741 gatcatattc tgtttgagag attttttcgt tttgaagcat cgtatacaaa acagtatctc
     1801 gtcttttctt cgcctgatct ggataaacat aaggatcata ggcggaaggg gcttgaggca
     1861 tcccagcaag caatgctgtt tgcggtaaag aaagttctga taatttctta ccaaagtaca
     1921 tctcagaagc tgtctccatc ccgtacaagc cgtttgacat atacacttta tttacgtagt
     1981 aggtcaagat ctcttgtttc gatttttttt gttccaatct tacagccatc cacgcttctt
     2041 gggccttgcg tttcagagtc tgatcctcgg cactggtgga gaagaaggaa agtttgatca
     2101 attgctgtgt caaagtactt ccgccttgca gtccgccaga ggtaaagtta gagagggcag
     2161 aaccgatgat ccgaataggg tcaaccccga tgtgtttata aaaacggcga tcttctaccg
     2221 acacgatcgc atcttctaat gttttcggta attcattcgc agaaattttt tcacgctttt
     2281 ctgcgcctaa atcttcgaat agttcgccat cttgtgtata tagtttggaa gatacggtag
     2341 cgtctaattt tttatccgtc aattcgggcg catcttttgc gtaataccag aaaagtccta
     2401 cacctgctaa aaagaggata cagaaaaatg agagtattcc taaaaggatc ttgataaata
     2461 atcctttttt atgtgttcca gaggactttc cagaaccttt gccagaattc ttcttcacac
     2521 tctttttagg agtaggctgt ttctgtcgtc tagagcttct agtttgttca tttgccattc
     2581 ttgctttctc cttcaataga taatgttgat aaatagaagt caaccgcgtc caaataagga
     2641 atccgcggag aaataccagt tttgatctct attcctagtt cttcgatcaa agataaaggt
     2701 agtgatttgc gtcctttttc cttttgggcg tcccagtaag caatcaaatg aacactattc
     2761 aaataaaaac agcgcttcaa agacgaaaac cataacaaga caaagcagac cccattttgt
     2821 tcgaggcatg ctttcatatg ttcgatttga tgggcatgga agtttttaaa gggaaaagat
     2881 gttttgttct tggtttcttt tgcttcaaag tccaagtaaa agcctcgata gacgccatta
     2941 tagtctgtag ttgaggcttg ggtaaagtag gcttccttga tcacagcagc acttctctta
     3001 ggatagtcta ctttcacgat ttggataggg gtaggctttt tatggataac cgccaacccg
     3061 cgggaaagat aatatttatt actttcattg attgcttctt caaagcgcat cccccgattg
     3121 ccgaattcag tgatctgatt tcttttttct tgaatttttt cgacgggaga ggcattctta
     3181 taataaggtt ggccatttgg atagcgcaat accatcttta atcacactcc taggccacat
     3241 tatagcaaaa aagcggatag aaagaaaagg aatctgagat ggataagtca aaagtaatct
     3301 atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc
     3361 aagttgttaa aaatgtttta aaaaaagaac tccatcagtt agcagaagaa gggctggaat
     3421 gggtacttgt cagtgggaat ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat
     3481 tgaaagaaga atatccggaa ctatctcttg gtattttgta tcctttttct gattttggta
     3541 ataactggaa tgaagggaac caagaaaaaa aacaattagc agaacaatta gcagattatg
     3601 tcgaagctgt tagccatcaa ccctaccaat cacctagtca gctaaagaat cacactcgtt
     3661 ttatacttga gcatacagtg ggctgtctgc tgatctatga tgaagaattc cctggaaaaa
     3721 cgaagtattt ttttgaagat gcacagaatt accaagcaga tcatccctac gaaattcggc
     3781 tgatcagtat ggacgatctt caaaatttta gtgaataatc gatattgtgt ttgatttatc
     3841 aacttttttc ctatacaatg aaaaaggtag aaatgttatt acgtaaatga aaactagagg
     3901 tgtaaatatg gcgaatttgg tttatagtcc taaagacatt ttacaacaag aattcaaaac
     3961 aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc ttagacaata tcatcaaaga
     4021 ttatgaagct tacagcaaag aactccttgc tttacaagaa gaaaacgatc gattgagtgc
     4081 aaaagtagct caattatcta aaactcaagg agcagctcaa acacgcgtgc aacaaacaga
     4141 ggcaccaaag agcgcagcag tgaccaattt tgatatctta aaacgcttgt caaatcttga
     4201 acgtgaagta tttggtaaaa agcttgatca acaagcaagt gcagtcaaac cagcacaacc
     4261 taatccaaat aactacacaa atgcagacac aagcttagat gataatgaaa aaacacgtca
     4321 attttaattt ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg acttgatcag
     4381 gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa
     4441 ccataagcta aggtactctt tggagtaacg gcaggaaaac aagctaaggc ttcggctatg
     4501 cttaagtatc cttgaaagtg ccacagtgac gaagcggctg gggaaaccca tccggtggaa
     4561 cgcggtaaac ccctcgagcg agcaacccaa acaatggtag gggcgctttt tgtctggaag
     4621 aagaacagga caaaaaggca gtataactgc agatagatgg ttaccgcttt gttttttctc
     4681 cctggaaaag caaagtacag aacatggctt atagaaaacc gcaaacatca gggaattagg
     4741 tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca tttgaaagct atttttcaat
     4801 acttcagatg aatcgttctt attacaaaac aaacttttgt atcaccggtt attcggaatt
     4861 tgagtaaaag tgtggggtag gactgatgtc ccacctcctt tttttataaa taaggagatt
     4921 gtgtatggaa aaaaacagga agttcaacct cgtcgcaact gcggcaagcg ggctagaagc
     4981 actggttggt aaagaattaa gagatcttgg aatcgaatgc caagttgaga acggacgtgc
     5041 acgttttgaa ggaacaatgg aaacgatcgc tacagctaat ttatggttac gtacagcaga
     5101 tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa
     5161 cgtaaaagca ttgccttggg aagattttct tccactagat gcagcttttc ctgtagctgg
     5221 aaaatcgatc aagtcgaaac tatatagcac acctgattgc caagcgatca caaaaaaagc
     5281 aatcgttgaa cgtctccgta cctattatca ccgtccagct tctgtaccac tgacggaaac
     5341 tggcgcacat tttcagttgg aagtagcttt gctgaaagac catgtgatgg taactctgga
     5401 tacgacagga ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt
     5461 gaaagaaaac atggcagcag cacttattat gttgacaaat tggcgaaaag accgtccatt
     5521 ttatgatcct gtatgtggtt caggtacgct ttgtattgaa gcagccttaa taggccataa
     5581 tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga
     5641 aatcatggaa aatgttcgat cagcagccga agaaaaagca gattatgata ttgaattaga
     5701 tattactggt tcagatatca atggacggat gattgaaatt gccaaagcca atgcagaaga
     5761 aatcggatta ggctcttcga ttacctttaa gcaacaagca gtcaaagact tcaaaacgga
     5821 taaagaatac ggggtcatcg tggctaatcc gccttacgga gaacgtctag gagaagaaga
     5881 aactgttcgg aaattatata aagaaatggg cgatgtattc cgtccattaa aaacatggag
     5941 taaatatatc ttgactagcg acttagcgtt tgaggaattt tacggacaaa aagcaaccaa
     6001 aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta ttccagtatt ggggaactcg
     6061 tccgcctaga aaaaaagaga aagacgaata atcttactaa ttgattcatt caattggatg
     6121 tttggaggaa tagggaatga aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct
     6181 tctacaacaa gctttaggaa tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc
     6241 tagtgcatac cgaagtgaag tagacagcta tctttacagc atttattttt caaaaaaaat
     6301 cggtccaaaa atcaaagaag ccattcgcta tttttctgaa catccggaag aattgtcgga
     6361 agttggaact gccgtttttg ccaaagtaaa agaagagtat gatttggaac atcgcgtacc
     6421 ggaagatctt atgagaaaat atacagctgc cgtttcttca gcacacggac aatggcaaaa
     6481 agctagagaa acacaacaat tttctgattt tcaacacgca ttgaatcaaa atattgaatt
     6541 aacaaaacag ttgatacctt actggagaaa agatgaaaag acgaattatg atgttttgct
     6601 gaatcaatac gaacccggga tgactgtcga aatccttgat aaggtatttg atcagcttaa
     6661 agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga
     6721 ctttttaaat cggagaatga cgaaaaaaca acagaagaga tttgtagtga aagtaatcga
     6781 acaacttggt tatgatttta gtcgtggtcg tttagatgac actgtccacc catttatgac
     6841 tggaattaat ccgaatgatg cacgaatcac gacacgatgg aacgaaacag atttcaaaat
     6901 ggctgttttt ggagtgatcc acgaggctgg tcatggtatg tatgaacaag acatcgatga
     6961 taaatatgcg tatacaccgg tttatgaagg tacatcaatg ggtatccatg aatcacaatc
     7021 gttgtttaac gagatcatta ttggaagtag tcgcagcttc tggaaaaagc aatatccttt
     7081 ctttcaagaa tgtgcagaag gaacattcga tgatattcct tttgacaaat tttatcgttc
     7141 attgaaccat acaaaatcaa gtttggttcg tatcgaggca gacagtttga cttaccctct
     7201 ccatatcatc attcgttatg aaatcgaaaa aatgattttc aatgaagggg tagatgttgc
     7261 tgaactacct gaaatatgga atcaaaaata tgaagagtat ctgggaatac gaccaacaaa
     7321 tgatgtggaa ggcatcctac aagacgttca ttggtcaggt gcaagctttg gttattttcc
     7381 ctcttatgca ttagggtaca tgtatgcggc acaattactt cattctttgc agcaagaaat
     7441 agatgtggaa aatgttctag cgtcagacga ttattctcca atcaaaaact ggatgtcaga
     7501 gcatatccat caatatggtg catctagaaa accaaatcaa ttaatcatgg atgcaacaca
     7561 tgaaccgctt aatccgcaat acctgatcga ctatatgaaa gacctgtatt ttaatgtata
     7621 tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat tcatctaatc tatagatgtt
     7681 ttcagctatt tacttaagaa agaagtggaa cgaatggata aattacaagt gatcgctgat
     7741 tatagtatgg accaattagc gcatgatcaa acaggacatg ggagcgatca taccaaaaga
     7801 gttgtcaagt tagctgaaaa aattttggag acggaaccgc aggcagaccg ctttgtgacg
     7861 cttgcagcag cttacctaca tgatacgatt gatgataaag tcgttaaaaa tgagaacgag
     7921 gcaaagcaac agcttcgtgt attccttcgc tctttgccaa tagtagagga acagatctcg
     7981 atgatttttt ccattatcga aaatatgtcc ttttctaaaa atctctctga agcggccgaa
     8041 ctatctctgg aaggtaagat cgttcaagac gctgatcgta tcgaagcatt aggagcaatc
     8101 ggcattttgc gtactgctta tttcggcggt ggacatgggc acccgatttt tgatcctgaa
     8161 ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa aaggcacgac agtgatcaat
     8221 catttttatg aaaaactgtt cttgctgtct gataaaatga atactgatta cggatatgaa
     8281 gaagccaaac gaagagaaac cttcatgcgt gaattcttag aagaattttt tctggaatgg
     8341 tctattgacg atgaaaattt tactccagac aattggatca aaaataacaa aaaatgatgg
     8401 aggttgtgac agaagtggtc agctccaaga tatttcggat cattttccgt aaaagttact
     8461 tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa
     8521 caatttcaaa aaatagcttc tcgtgtttga tgaatattgt aacttgtgat tcggaattgg
     8581 caggagaaca ccgtttattc ggaagaatag gactgtgaca agacttttgt cacagtcctt
     8641 ttttcgaatt caacaagtaa aacttttaag aaatattcag agagtaaaat taaaaattgc
     8701 tgataaacga agatggaaat tttatgtatt tttattggtc tatgcaataa ataaattaaa
     8761 tattcagaat acaattgatt ttaacgaaag ggcttctatc aatggttgtg ttgtatttat
     8821 ctgcccaggt agagtaaaat agatcattat ctaaaaggta attgttcata ctatataagt
     8881 catttgaaag taaaaagata ctgtaaataa aaaatgtggt taaataattc tcataatcag
     8941 tgtaagcgtt atcaaaaata tagcatactt gctttgtaga tgaatgacac gatatatgtt
     9001 tggtatgttc tgcaagacag atgaatatgg aggtggagac aatgtcttcc aaagaactaa
     9061 caatgtattt agagaaagaa ttggatcaac tgaaagaaaa ggggttatac aatacgatcg
     9121 acatactaga aagtgaaaat ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc
     9181 tagcatccaa caattatctt ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg
     9241 aagcaaccga aacttatggt gtaggagcag gagcagtccg aacgatcaat ggttcgctga
     9301 aaatccatca gcaattagaa gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg
     9361 cctttcaatc tggattcaac tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag
     9421 atgctatctt atcggatgaa ttaaaccatg catctattat tgacggttgc cgcttatccg
     9481 gagcaaaaat catccgaatt aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg
     9541 aagcgataga aagtaagaaa tataaaaaaa tcatgtatat cacagatggt gttttctcca
     9601 tggatggaga cattgcacga ttgccggaaa ttattccgat tgttgaaaaa tatggactga
     9661 tcacctacgt cgatgatgcg cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca
     9721 agcattttgg attaagcgac aaaatcgata tgcaaatggg cacgttatcg aaagctatag
     9781 gtgttgttgg gggttatgta gctggatcga aaacactaat cgactggttg aaagctagaa
     9841 gtcggccatt cctattttct acttcgctaa ctccaggtgc agctgcagct gcattggctt
     9901 cgattacttt gatgcaagaa catccggaac ttgtcgaaaa agtttgggaa aatgctaatt
     9961 attttaaaga agaattgaaa aaagtaggct ataacatcgg tatgtctgaa acacctatta
    10021 ctccagtcat tttgggagac gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac
    10081 atgggattta tgctaaacca atcgtttatc caactgtgcc tcttgggaca ggcaggatca
    10141 gaaatatgcc gactgcggaa catacgaggg aaatgcttga tgaagcagta gcagtttatc
    10201 aaaaaatagg aaaagaaatg gaaattattt aactaaattt agttttttcg tctatttaaa
    10261 attagagaac atgttcacgc atatattgtt aatcatctga agatagtagt aaaaaagatc
    10321 agggaggatc atcatgaaaa aagtattggt gacaggatgt ttaggacaaa ttggttcaga
    10381 gttgatcaca cggctacgtc aagaactagg cagtgaaaat gtcattgcga cagatatacg
    10441 catgcctgaa gtcattgaga aaaacgatat ctttgagatc ctagatgtaa tggattacga
    10501 aaaaatgcgc agcatagcag aacaatatca agtggacaca ttgattcatt tggctgctct
    10561 tttgtcagct gttgcagaag caaaaccaca attagcctgg aatttgaacg tgactgggct
    10621 ggtcaatgca ttggaagtcg ctagagaatt ccagctgaaa ttttttacgc caagttcaat
    10681 cggcgcgttt ggtccaaata ctccaaaaga tcatacccca caagatactc ttcagcgtcc
    10741 tacaaccatg tatggggtaa cgaaggtagc tggagaacta ctatgtgatt attattatgt
    10801 gaaatatggc gtagatactc gtggtgtccg tttccctgga ttgatttctt atgcaacctt
    10861 gccaggaggc ggtacgacag actacgcagt cgatatttat tattcagcaa tcaaagaagg
    10921 aaagtacact agtttcattg atcaaggtac atcaatggat atgatgtata tgccagatgc
    10981 gatcgatgcc attataaaac tcatgaatgc agatcctgat cgattggttc atcgaaatgc
    11041 cttcaatata tcggcgatgt cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat
    11101 cataccagat tttgaaatgg attatgatgt ggatcctgta cgtcagtcaa tagcagaatc
    11161 ttggccaaat tcactagatg tttcttgtgc gcaagaggaa tggggctttt cgccgaaata
    11221 cgacttagat gtgatgacaa aggatatgtt ggaaaaactg aaagaaaaat tggtagagac
    11281 ggtataaaac aagaaccaca tccaaaaagt tttttatact tttgggattg gctatggtat
    11341 gaaaggggac aagggatgga agaatatcaa gccacgccag taaaagaagt aaaaatagaa
    11401 aatgaattaa aacgtacgat gggatttttt actgctttat cgactgttat gggcacagtg
    11461 atcggtgcag gggtattttt taaagccgca agcgtagcag aagttacagg atccgccagt
    11521 ttgcatatgt tttcttggtt tctcggtggt atgatttctg tctgtgcagg actaacagga
    11581 gctgaactgg cagctgctat tccagaaaca ggagggatga ttaaatatat cgaacggatt
    11641 tacggaaata cagcagcttt tttattgggc tgggcgcaag tcgtgattta ttttccagca
    11701 aatgtagcag ccttgtcgat cattttcggt acacagttcg tcaatctatt tggtctttcc
    11761 cagtcaatga ttgttcccgt agctgttaca gctgctgtat cgatcctgtt gatcaatttt
    11821 ttaggttcta aagctggagg ggcttttcag tcgattacgc ttgtctgcaa gcttattcct
    11881 ttgtttgtga tcgtcatttt cggtttgttc cgacaagaag gagtagactt ccaactgttt
    11941 ccgatccaag caggagaaaa tctttcattt ttctcagcct tgggagcggg cttgctagca
    12001 acgatgttcg catacgacgg ttggatacac gtaggaaata tttcgggaga attgaaaaag
    12061 ccggcgaaag atttgccaaa agcaatttct ttaggaatca tcggaatcat gatcgtttat
    12121 ttattagtaa atgcggtgtt cttaagaacc gcatctatcg acggagtcgc aggaaacagc
    12181 aatgcggcaa gtgacgttgc aaaaatgatt tttggcggct tcggtggaag actggtcaca
    12241 gttggtatct tgatttctgt ttatggaacc atcaatggct atacccttac tggaatgcgt
    12301 ctcccttatg ttatggcaaa agaaaacaac ctgcctttta gcaagctctt tgctaaactt
    12361 catgataaaa ctaaagttcc tgtagccgca ggtatcttag agctagtcat cgctattggc
    12421 atgatgatga tcggtggttt tgatacacta acggatatgc tgatctttgt catttggatt
    12481 ttttatacga tggtctttgt tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt
    12541 cgaccataca aagttccaat gtatccgttt attccactag tagcaatcat tggagggact
    12601 tttatcgtat caagcacgct tatcactcag acctttttag cttcgatggg gatagtattg
    12661 acacttgctg gtatcccgat ttatctctat ttaaaacgta gatagctaaa acattcgaat
    12721 tttattagaa ctgcacttct ctgccaaaca aaatatttta tttaaatgag gagtacgtgg
    12781 aagaaatgtc aaaagtactc gttaattttg atcacgtaaa tgtcgttttg tctacgatga
    12841 aatagcaaag taaaagacag aaatcgggct caaccgattt ctgtctttta ctttgatttg
    12901 cgtaactgaa ctattcggcg tacgccgcga taaatattta ataaaaccaa gcaaccagaa
    12961 atgaaaatgg gaagccaaat cagatagaca gggtagccaa gcactttaga caaagcatgt
    13021 ggagtcattt tatccatttc aagaagtgct tgctgccaag tctgaagatt gaagactagc
    13081 atgaaacccg aaataatcag gagaataaat cctaaataat gaattgtagt tctttttctt
    13141 acataagcga tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac
    13201 atattcagca tctcgacacc ccctttgtgt ttatcttact ttactttgaa agaaatgaga
    13261 aataatagcc tcgttacttg cttgaaacag caaaggactg ctaagataag agagatacaa
    13321 agaatcaagg aggagtcata tggcaagtta tatcgaaccg attcgttttg cgctgattgt
    13381 ttttccattt ttagccttag cgatctcatc tgtttttttt atttatgaat atcgaaagta
    13441 tggaacattt ctagtaacaa gggcagttat tttgtattct ttcgtttttt atttattgtg
    13501 tgcgtatttt ttggtgatct taccacttcc ggcaaaggag acggttgccc aaatgacagg
    13561 gccaaagatg gagctgcatt tatttgcttc atggcaacat ttcagagaca acacggtatt
    13621 ggtcttgtct gaacctagta cctacctgcc ggcaatgaag cagagtgtat ttttggaacc
    13681 agcttttaat gttttattac tcataccatt tggcatttat ttaagatatt atttccggct
    13741 ttcttttatg aaaacgatga ttgctagttt ctgcctttct ctattttttg aattgacgca
    13801 gctaagtgga ctttatttta tctacccaag accataccgc ttatttgatg tgaatgattt
    13861 agtgacaaat acattgggtg gaatcattgg ttttgtcgtt acgccgttat ttacatttat
    13921 gcttcctaca agaagacgaa tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt
    13981 gactcgacgc ttggtcgctt atttgatcga ctggacattt ttatctctta ttaatttggt
    14041 agcggcgatt ttctttaaaa ttgcgttcga ttcaggggca gttacttctc atatctggtg
    14101 gcttttatta gaagtagggg tatactttat gattgtaccg tttataacca atggatggac
    14161 acttggcaaa aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt
    14221 aaaagcattg atgatccgct acgggtatct gtactttctt ttttatggtt taagctggtt
    14281 ggggcagttg cttcagcaaa tcatgaattc gacaacgaat acggtgttgc agatgagtgc
    14341 agtattcatt tctctcggtg tcttgttact acaaggtatt tttattttaa atatcttgct
    14401 atcatttttg agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca catatactgt
    14461 tagcacgatt ggccaaacaa aggatgatga ttctcgaaat tagctgagca aaaacgcttt
    14521 gtctgtgtta taatgccaaa gtcgtgataa tttaaagaga agaggtgcac gaatgaataa
    14581 ttttgatata ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa
    14641 acagcccaca gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat
    14701 tgggcaatct caaactggta caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa
    14761 aatcaatgca gaaaaagagg aagtacaagt tgttattaca gctccaagta gggaactagc
    14821 tgcacagatc tatgaagcag ctacacaaat cgctaaattt tctacaccgg agatccgtgt
    14881 ctcgaatttt gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca
    14941 gccacaggtt gtagtaggca cgcctggtag aatattagac atgatgaatg aacaagcgct
    15001 gaagattcac accgcatttg catttgttgt cgatgaagcg gatatgacat tggatatggg
    15061 ttttttaaat gaagtagacc aaattgcaag tcgattacca gaaaagctgc agtttttagt
    15121 tttttctgcc actattccag aaaaacttcg tccattctta cgaaaataca tggaaaatcc
    15181 aatcattgaa gaaatcaaac caaaagcagt tatttctgaa gacattgaaa attggctgat
    15241 ttcaacaaaa ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc
    15301 ttatttagca atcgtatttg ccaatacaaa acagcatgtg gatgaaattg ccgattactt
    15361 gaaaaaccaa ggcttgaaag tagcaaagat tcatggagac atcaccccaa gagagagaaa
    15421 acgagtgatg cgacaagtac agaatctgga gtatcaatat gttgtagcga ctgatttagc
    15481 tgctagaggg atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga
    15541 tctagacttc tttatccatc gtgtcggacg tactggacgt aatggcttaa aaggaacggc
    15601 cattacgtta tattcgccaa atgatgagca agccattgat gcgatagaaa aattgggcgt
    15661 ctcatttgaa ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag
    15721 acgtgccaaa cgagaaaaat caaaagatgt attagatccc acgctgattg gtcttgtgaa
    15781 gaagaaaaag aaaaagatca agcctggtta taagaaaaaa atcgattggg caattgcaga
    15841 aaaaaataaa aaagaacgca aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa
    15901 taagaaaaat tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc
    15961 ttgtttttaa gctagacttt ttttcattcc gatacaaagt acttcatctt caataaaagg
    16021 ttctgtgcaa gtatgatacc cttgtttttc ataaaaatct acagccgtca tttcagctac
    16081 tacataacta gtctgacaac cttctgaaac cgcccgtttt tcagcagctt caagcaactg
    16141 accgcctaac ccttttcctc ggtattcttt tgctacgcaa aatcgttccg gcttgaagca
    16201 agtaggtgtt ttcttttcat aacgcaaagt agctactggt cttttttgat caagaaatag
    16261 ataataggtt tgttgcggga tatcttttcc gtcgaattct agttctgcag gaatcccttg
    16321 ttctaataca aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc
    16381 tccaactatt tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc
    16441 tttattctta tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt
    16501 gagatattga cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt
    16561 tgttttttaa gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa
    16621 attgctccct ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa
    16681 gaggtaatga ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg
    16741 tgatggtgtt tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt
    16801 agtgcacaag ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa
    16861 cctagtgcgt ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt
    16921 gcaacattga aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat
    16981 gcccaaaaat ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat
    17041 acgatgtttg agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc
    17101 cactgggcat gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt
    17161 tatgtaacag tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc
    17221 ctgccactag atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca
    17281 tgtggtccgg atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa
    17341 gacgatcctg aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta
    17401 gtattttcag aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac
    17461 attgatacgg ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac
    17521 tttgagacag acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta
    17581 aaatatggtg aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc
    17641 gccctttcat ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc
    17701 ttacgccgtt tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca
    17761 ttcttatatc gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta
    17821 cttcaacaaa aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa
    17881 acaatcaatg aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac
    17941 gggacgactc tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta
    18001 gaattaacgg aagaggtggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa
    18061 aaagaaatgg atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg
    18121 ggggtacaat ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca
    18181 gaacaagaat cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa
    18241 gtcgcagaag gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt
    18301 gggcaagtag cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt
    18361 acagatgttc aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca
    18421 atgagtgaag gacaaaccta tcatctattg atcgatcaat cgatgcgcaa tcggatcatc
    18481 aaaaaccata cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat
    18541 gcaaatcaag ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt
    18601 ggtcaaatca cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg
    18661 gaagctatcc cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca
    18721 atggctttgt ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg
    18781 tcaatcgaac tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa
    18841 atcgtttctg aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa
    18901 gaagcgtacg aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt
    18961 aaatcacctc aattgaaaga agttgtttca aaagcggaac aattgcaaca gcaagtaaga
    19021 gagctacaaa aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat
    19081 gtattcaaag atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg
    19141 aaagatatga atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat
    19201 attcttgttt tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa
    19261 gaatcaaatg aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta
    19321 ggcggtggcg gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt
    19381 attccagatg cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt
    19441 tgacagaaaa acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac
    19501 aagtcttgcc ctcatacttt tttcaattgg atggcgcatc taatcaaatg gtcggattcg
    19561 gatggtcttt agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg
    19621 tgaaaaaatg aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc
    19681 aatcatcatc gtacaagcga tggttccttg gctggggttt attccattag gattcattag
    19741 tttaacaatt atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg
    19801 catggtgatt ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac
    19861 aacgccattt gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact
    19921 tgttggctta gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac
    19981 gatcagctca gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg
    20041 attcatggga ttattttata caggtgcaac ggcagatgcc tatggtgtag actctgctct
    20101 tttatttaaa accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc
    20161 gggaatcata acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga
    20221 ataatgacca tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag
    20281 aggaaaacaa tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa
    20341 aaaacttggc taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa
    20401 aaggagtact agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc
    20461 taatgatcgg gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat
    20521 cagatactgc ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag
    20581 atcaggaaca tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga
    20641 tcatcattac ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc
    20701 cagaaaatgt ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga
    20761 aatttgcaca aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga
    20821 gagctacagc aacagatagt tataatgatg tctttactac actggctgta ttgctttcag
    20881 cagcaatcga atgggtaact ggatggcgta ttgatggata tataggtttt gctttagcat
    20941 tatatatcct ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa
    21001 gtcgcccaac gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata
    21061 tacttggcta tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag
    21121 tccacatcga agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga
    21181 tagaaaaaga ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc
    21241 ctgttgataa cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa
    21301 caggtacagg gcttaatatt catgatttta gagtaattca tcaaggaaaa acgctgcaat
    21361 ttgatgtagt ggtccctgag aaattccagt ttgttgatac agaactagaa agaatcttac
    21421 gtagtcaaat agaagaaaaa atcggagatt accaattgga aattacgttt gatcacaatt
    21481 atcttttata ttaaatgaga aactataaaa agactcctta aagtttttaa tttaaagagt
    21541 ctttttttct tttaattgtt tatggagagc ctgacgtgca agattatctg ctcctttatt
    21601 tttgttttcg ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa gttcttggat
    21661 tctttgaagg tagggaagga agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac
    21721 tagtgttttg ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt ttttctttag
    21781 aaattctaaa gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc ctatagaaag
    21841 cgggatagat agctgttctt gtttaccttg atagacaatc aagattccac ctccactagg
    21901 accagggttg ccttttgtag aggcatcaat gtatactttt aacataaaaa gagattctcc
    21961 tttttgttca aactaatgtt atggtaagat tatatcatta ctgaatcggt gagaggttag
    22021 agaatgaaaa aaaatattcg atggcagcct gaactggcac atacgatcat ctattggtca
    22081 ttgaccttga tcattctgtt ttttagtctg attctttctt tagaaaacac aggtccttat
    22141 tggaaaagca attttgtttt aggtattttt cttgtttttc tatttctagg tttgcggcgt
    22201 tcttttattt taaaatcaaa ctctgtgatt atacgttatg ctgctttttg gaaagaccga
    22261 gaaatatctg tcggacagat tcgatctttg acgccaacta atactggggt gcaaatgatg
    22321 ttgcaagatg agaaagcccc atctgttttc atgatgcgaa aaaaagatca agtagtcctt
    22381 tttgatcatt ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa
    22441 aaaagaaaaa gctaatttta aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga
    22501 aaagagtgag acgtacggtt catcccgttt agcgtctcac tctatttgtt tatctattca
    22561 tgcatactag tatcggtagt ctgaacgttg cctttcttga catgggcagc ttgtgttcct
    22621 cgattccctt cgatgatctc aaattcgacc acttgatttt cttccaaagt tttgaatccc
    22681 tcttcttcaa tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata actgataaag
    22741 ccgtatcctt ttttgttgtc aaaccatttt acaattcctg tggtcaaagc actcacgacc
    22801 tttctaaagt ctgatcatag ccttcattat aaagagtatt tataagtacg tcaaataaat
    22861 taaaaacctg taattttatg ttttttttct gaataaaaaa aggtataatt ggcatatctt
    22921 aacaaagaag atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga
    22981 tctgcaaatt tcacaagaaa cagagctaga accgattgtt gaagtggcta gtaaattaga
    23041 tcttaaggaa gacgatttgg aactgtatgg gaaatataag gcgaaaatca atttttctgg
    23101 aatcaatcga tcaaaggata acgctgaggg gcatttgatc ttagttactt ccatcaatcc
    23161 aacaccagct ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg cgctcaataa
    23221 aatcaataaa aaatctgtta ttgcattgcg agaaccctca cttggtcctg tgatgggaat
    23281 caaaggtggt gctgcaggcg gaggatatgc acaggtattg cctatggaag atatcaactt
    23341 gcattttact ggtgacatgc atgctatcac aacagctaat aatgcattat ctgctctttt
    23401 agataatcat attcatcaag gaaatgaatt gaacatcgat gcccgccgag tgatttggaa
    23461 acgggtagtc gatctcaatg atcgtgaact gcgtaaagta attgttggct tgggtgggcc
    23521 aatccaaggt gttccaagag aagatgggtt tgatatcaca gttgccagcg aaatcatggc
    23581 tatcttgtgt cttgcggcag accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt
    23641 cggctataca tttgaccgtc agcctgtgac tgctggagac ttgaaagcag aaggcgctct
    23701 ggcattactg ctgaaagatg caatcaagcc gaatcttgta caaacaattt acggaacacc
    23761 agcttttgtc cacgggggac cgtttgccaa tatcgctcat ggctgcaata gtattttagc
    23821 taccaagaca gcattgagat tagcagatta cgttgtcaca gaggcaggat ttggtgcaga
    23881 tcttggcgga gagaaatttt tagatatcaa agtacctaac ttgaaaaaag cgccggatgc
    23941 tgtggtgatt gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga
    24001 acttaaaaat gagaatcttg atgctttgaa agtaggtttt gcaaatttaa agcgccatat
    24061 acgtaatatg gaacaatatc agctacctgt gatcgtcgca atcaatgaat tcgtaacaga
    24121 tacagacaat gaactcacac tgttggagca cttgtgtgaa aatcagggga ttcttgccaa
    24181 gagagcgagt gtatgggcag atggagcaga aggcggcgtt gatttagcaa aagctgtagt
    24241 acgattgatt gatagaaaag aagctaatta caagccatta taccgcctag aagaaacgat
    24301 acaagaaaag acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag
    24361 caaaaaagcg caaaaacaaa tagaagagtt tacgaagaat ggatgggaca aactgccgat
    24421 ttgtatggca aaaacacagt attcattttc tgatgatcct tcactgctag gtgcaccgga
    24481 agatttcacc atcacgatcc gcgaaatcat cccaaaatta ggggcaggat ttttagtagc
    24541 cttgactgga gatgttatga cgatgcctgg tttgcctaaa aaacctgctg cattgaatat
    24601 ggacgtgaca aatgacggag aagtgttggg attgttttaa gcttgattat aatctaccat
    24661 cgaaggagct gtattttgac tacagcttct ttttttagac gatagggaat taattttgtt
    24721 tacactagga tcattctttt tctttattgt attttgagct atactaaagc ataataggag
    24781 ggtatgatga aaacgacatt tataagaaaa accggttttt acggtatggg tagctcgatt
    24841 cagctaagaa tcaacaatga aaaacgcagt gttctcagtc atgaacaaaa gataacgctt
    24901 gatctggaag tgccctttac gatgcaggtc accttttatt ggctaaaaag ccccgtttat
    24961 acagtgaaag aagcaaagcc tgcttatgtg atcacaatga attacttgat tcttcaaatc
    25021 tatccgtttt tatttctagc aactgggttg ggggccgttt tcctacaaaa tttatttttc
    25081 agtttttttg cagttattgt gttgattgcg ttctttttct atataaaaaa ccgggcatat
    25141 ttgatcaagg aggaaaatta tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat
    25201 cgaaaaatgg atgagagacg agatgggaaa tgcacgaaat atgggattta ccgaacttgt
    25261 cagacgccta gctcaaagaa aacaattaat gatcagaaaa tacgaagatg atcttttaca
    25321 gcttgctcag ttaaggaatg cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc
    25381 agagccaaat gattggacag tcaagcgaat ccagctgatc gaacaagaac tgattcagcc
    25441 tgaaaccgtg ctgcctcgat ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt
    25501 acttgaactg ttaaagatag tagcagaaaa aaggtattca caatttccgt tatacaataa
    25561 aggaaaattt gtagcattga ttactttacg taatatcggg ttttggctag caaaagaaag
    25621 ccaaaaaggt ccggttgatt taacaaataa acgagcgttg gatttgatca tccaaaacgg
    25681 aaagtacaca aattatcatt ttgttccagc taaaacacat atttacgagg ttgaagcgat
    25741 gtttcgtgag caagggactt tggaagccgt acttatcact aaagatggga atcccgatgg
    25801 taatctgctc gggatcgtac gacctcgtga tatttataaa caaatagaaa aggattgatt
    25861 ttttgttaat tatttattgg atattcagcg ctttgctagt aggagtggat cagttggtca
    25921 aatggtggat cactgataat ctagcgcttg gagagacgaa aaatcttata ccaggtattt
    25981 tgtcactgaa tcatattcga aacactggag ccgcatggag tatgctagaa gggaagatgt
    26041 ggttctttac tatagtgaca ctcattgcag tcgttgtgat tctcacattg atgattaaaa
    26101 atcgtgaaaa aggtaatcgc tggttcatga ttggattgag tttgattcta gctggtgcga
    26161 ttggcaattt tatcgaccgt attcgtcttg gctatgtagt agatatgttc cagacagatt
    26221 ttatgagctt tccgattttt aacgtagcag atgtgacact tgtatgtgga gtgatttgta
    26281 tgttaattta tattatttta gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac
    26341 gacgattcac catgaatctg gtaggatcga taaagtattg actaatttat tcaccgatta
    26401 cagtcgttcg caaattcagc tgtggctgaa aaatggagcg gtttctgtca atggacagcc
    26461 agtcaaagca aattacaaag taaaacaagg agacgagatt actgtcttga tccccgaacc
    26521 agaaacatta gatgtggtcc cagaaaatat cccgttgacg atcgtttacg aagatgaaga
    26581 tgtagcggtg ataaacaaac ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca
    26641 tggcacaatg gtcaatgcgc tgatgtacca tatcaaggat ctttctacga tcaacggcgt
    26701 tattcgtcca ggtattgttc accgaatcga caaagatact tccggactgc taatggttgc
    26761 caaaaatgac caggcacacg aatcattagc tcagcaatta aaagataaga cctctttgcg
    26821 caaatatgtt gctttagtcc atggagagat tcctcacgaa aaaggaacca tcaatgcgcc
    26881 aatcggccgt tcgaaggaaa accggaaaat gcaggcgata agagaagatg ggaagtcggc
    26941 tgtcacacat tttactgtat tggaacgttt cgaaggattt actctggttg aattaaaact
    27001 agaaaccgga agaacacacc agatccgtgt gcatatgaag tatatcggtt atccgttagc
    27061 tggagatcca gtatacggac cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc
    27121 gcaaatgtta ggttttacgc acccgactac aaacgaaaag atgatctttg aagcacctct
    27181 tccagaagtt tttgaaaaaa ctttagaaaa attaagaaaa aacattgcat tttaatttag
    27241 ataacggtat agtaattaac aacgaaagca taaaacttaa tagaattcct ttaagtttag
    27301 tcccgtgagg ctaagaaggg tgggagcaag gatacgtatg gtgcgccata agtagattgc
    27361 tcattgacat agtatagcta tcagtgtatc agtatacgct cagtctaaac attcctcctg
    27421 tcctcttagg gcaggagttt ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa
    27481 agaagtagta gatcaggtaa ccatgaagcg agcattgact cgaattactt atgagattat
    27541 cgagagaaac catagtatcc aagatatcgt attggtaggt atcaaaacac gaggaatcta
    27601 cattgcatca agaattgccg aacgcctgaa gcagctagaa gatatcgata ttcctgtagg
    27661 agaactggat atcactttat accgagatga caaaaaagaa aatccagaag aaccggaact
    27721 tcattcttct gacatcccag tttccttaga aggaaaagaa gttattttga ttgatgatgt
    27781 tttgtataca ggacgaacga ttcgagcagc aatggacgca gtaatggatt ttggcagacc
    27841 aagaaaaatt tcattagcag tcctggtcga tcgtgggcat cgagaattgc caatccgtgc
    27901 agattatgtt gggaagaaca ttccaacatc tcgggcggaa gagattttag tagagatgca
    27961 agagcttgat ggacaagatc gcatcatgat tttgaaagag gaagattaga ggagagagaa
    28021 acatgtcgga gaaaaaagga tttcataatg atgacgtggt gttagatatc cacgatcgcc
    28081 caaccccagg acattgggta ggattaagtt tacaacactt gttcacgatg ttcggagcaa
    28141 ccgtgttagt ccccatctta gtaggtatcg atccaggtat tgcgctagtc agttcaggac
    28201 taggtacatt ggtgtactta tttactacta aaggaaagat acctgcgtac ttaggaagca
    28261 gcttcgcttt cattgcagcg atgcagcttt tgatgaaaac agatggttat ccagcgattg
    28321 cacaaggagc agtcacaact ggattagtct atttgattgt tgcgctgatc atcaaaaaga
    28381 tcggttcagc atggctggat aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg
    28441 ttatcgggtt gggacttgcg gcaaatgcag caacaaatgc tatgtataat cagggcacgt
    28501 atgatttcaa gtatattgct gtagcgctga tcacattagg attaacaatt ttcttcaaca
    28561 tgtggctaag aggttttctc ggattgattc cgattttact aggtattatt agcggataca
    28621 tcgtagcctt gctgtttgga atcgtggata ctaagccaat cgtagacgct gcctggtttg
    28681 ctgttcctaa ctttgagatt ccctttgtac aatatgaacc aaagctatat cttggagcaa
    28741 tcacaacaat ggcacccatc gcttttgtga cgatgacaga gcatatcggc catcttatgg
    28801 tattgaataa actaactaaa cgtaatttct ttgaagaacc gggtcttcat aagacgttga
    28861 caggtgatgg attagcacaa atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt
    28921 acggtgaaaa cattggtgtt ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg
    28981 gagctgcagt atttgcagtc attttaggat tcgtaggaaa attaagcgct ttgattttaa
    29041 gtatccctgg accagtcatc tcaggaatca gttttatctt atttggagta attgccgcaa
    29101 gtggaatgaa gattttaatc gagaacaaaa tcgattttga taaaaagaaa aatctgttga
    29161 ttgcttcagt tatcttagta gtaggaattg gcggactcgt ctttgaagta ggcacattta
    29221 cattgtcggc aatggcatta gcaactgtac ttggcatcat tttaaatctt atcttaccag
    29281 aaacttcacg aagcgaagaa aactaaggag gaaacattat gatcatcaaa tcagaacgta
    29341 tcagcttaaa gcatttattg accgttgagg cacttactga tcaagaagtt atgggcttga
    29401 tccgcagagg gcaggaattc aagcaaggtg ctaattggac tccgcaaaag cgccagtact
    29461 ttgctacaaa cctctttttc gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg
    29521 agaaaaaact agggctagaa gtgattgaat ttgaagcaag cacaagctct gtccaaaaag
    29581 gggaaacact atacgataca gtactgacga tgtcagcatt aggcgtagac gtagcggtta
    29641 tccgtcatgg tgatgagaac tattatgacg aactgataca aagtaaaaca atccagtgtt
    29701 cgattatcaa tggaggagac ggaagcggac aacatccaac ccaatgcttg cttgatttga
    29761 tgaccattta tgaagaattt ggtcattttg aaggtctgaa agttgccatc gtaggtgata
    29821 tcactcactc tcgcgtagct aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag
    29881 tattcttctc aggtcctaga gaatggtacg acgatgaatt cgaagtgtac ggacattata
    29941 tgccattgga tgaactacta gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg
    30001 aacgccatga tggaaaagaa agtttctcaa aagaaggcta ccatcaggaa catgggttaa
    30061 cagttgagcg tgcgaagaag ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta
    30121 accgtgatgt agaattagct gattcattag tggaaggatt acagtcgcgg atcgttcagc
    30181 aaatgagcaa tggagtattc gtcagaatgg caatcttaga agcagtgcta aatggaaaag
    30241 cgtaattata ggaggatgaa aaaatgaaaa cactcattaa aaacggtcag atcaatacac
    30301 gaaaaaatat gacgacacca gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg
    30361 gtacaggatt ctcagaagcc gaatttgatg aagtgtttga tgcaaaggga caattgatca
    30421 cacctggttt agtggacgtc catgttcatt tgagagagcc aggatttaca tacaaggaga
    30481 cgatcgaagc tgggactcgc tcagctgctc gtggcggttt cactactgta tgcgcgatgc
    30541 cgaatttaaa tcctgtacct gatacggcag aaaagctgag acaagtatat gacatcatta
    30601 gaaaagatgc agttgtgaag gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg
    30661 agaaattagt tgatcaagaa gcactgattg aagaaggtgc ttttgctttc acaaatgacg
    30721 gtgtaggtgt ccaaacagct gggacgatgt atctggctat gaaagaagct gcaaaaaaca
    30781 ataaagccct agtagcccat acagaagatg aatctttatt atttggcggc gttatgcacg
    30841 ctggcaagaa ggcagaagaa cttggtcttc ccgggatctt aagcgtaacc gagtcttctc
    30901 aaattgctag agatctgttg ctagctgaag caactggtgt tcattaccat gtttgccatg
    30961 tttctactaa agaaagtgtc cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa
    31021 cagcagaagt ttcaccccat catcttattt tgatcgacga agatattccc gaagatttcg
    31081 gcttttggaa aatgaacccg ccgctaagag gacgggagga cagagaagct ttgatcgaag
    31141 gcttgcttga cggtacgatc gattgcatcg ctacagacca tgccccccat ggattagaag
    31201 aaaaaagcca aagtttcatg aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc
    31261 aactgatcta tactcatttt gtagaaactg gtcgattcac gttggaacaa gtgatcaatt
    31321 ggttggcagt taaaccggct gagatttttg gtttgaatgc aggaacgcta acggttggtg
    31381 cccccgctga tgtagcagtt tttgatatta cacaaacttg cacgatcgat aaagaagact
    31441 ttttatcaaa aggggagaat acacctttta tcggctggaa agtaaaaggc gaaacacaaa
    31501 tgacttttgt caacggaaaa cttgtgtggc agaagggaga ataaaagtat ggaacgtttg
    31561 cttatattag aagatggaac agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc
    31621 ttcggagaaa tcgtttttac aacaagtatg actggttatc aagaaactat cactgatcaa
    31681 agttttaacg gtcaaatcat tacatttacc tatcctatgg tgggtaatta tggggtaaat
    31741 agagatgatt atgaatcgat ttctccaaca tgcaaaggag tagtagtaaa agaacatgca
    31801 cgtgtagctt caaactggcg aaaccaaatg acgctagatt catttttgaa acaaaaagga
    31861 attcctggaa tatcaggtat cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga
    31921 acgatgaaag ctagtatagt tgatgctgga gattccttcg aacatgcatt tgatcagctg
    31981 aaagcaaccg ttctagcaac caatcaagtg cagcaagtct caactaacaa accttatcca
    32041 agtccaggaa caggtcggaa agtcattgtc attgacttcg gcttgaaaca tagtatttta
    32101 cgagaattga gtaaaagaaa ctgtaacctc acagtacttc cctataacac agatgccgag
    32161 acgattttag cgctatcacc agacggagtc atgctgacga atggacctgg tgatccaaaa
    32221 gatgtgccag aagcaatcga aatgatcaaa aaaatccaag gaaaagtacc gatttttggt
    32281 atttgtctcg gtcaccagtt atttgcttta gccaacggtg cagataccta taaaatgaaa
    32341 ttcggacatc gaggattaaa tcacccggta cgagaagtag cgaccggaag aattgatttt
    32401 acttctcaaa atcatggcta tgcagtcgat gaagccacga ttgatccaga aaaactcatc
    32461 gtaactcatg tagaagtaaa tgacggaaca gtagaaggag tcaaacacag agattatccc
    32521 gctttcagtg tgcagtttca tccagatgct gcccctggtc cgcatgatgc actacattta
    32581 tttgatgaat ttatggaaat gatggacgca ggaaaggagc agtaaacaga tgccgaaacg
    32641 tacagatatc aagaaaatta tggtgattgg atctggtccg atcatcattg gtcaagccgc
    32701 agaatttgac tacgcaggaa cacaagcctg ccttgcctta aaagaagaag gatatgaagt
    32761 ggttttagta aattctaatc ctgcgacgat catgacggat aaagaagtgg cagatcaagt
    32821 atatatcgaa ccgatcacac ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga
    32881 tgctctcctt ccaactttag gcggacagac aggcctcaat atggcaatgg agctggcagc
    32941 atcaggtatt ttagaagaat tgaacataga acttctagga acaaagcttt ctgcgatcga
    33001 tcaagcagaa gatcgtgatc tattcaaaca gttgatgaaa gaattgaatc agccaatccc
    33061 cgagtctgaa atcgtcaata cagtagaaga agctgtagat tttgcaaata aaatcggcta
    33121 tcctgtcatc gttcgtccag cctttacttt aggcggaact ggtggaggta tgtgtgaaaa
    33181 tgaagaagaa ttacgtatcg tatcagaaaa tggattaagt ctttcccccg ctacccaatg
    33241 tttgattgaa cgaagtattg caggttttaa agaaattgaa tatgaagtga tgagagattc
    33301 ggctgataat gcaatcgttg tctgcaatat ggaaaatttt gacccggtgg gtatccatac
    33361 aggtgattct atcgtatttg cccctagtca aacattgtct gaccatgaat atcaaatgct
    33421 tcgtgatgct tctctttcga ttattcgagc acttaagatc gagggaggct gtaatgtcca
    33481 actcgctctt gatccacata gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc
    33541 tcgttcatct gcgttagcaa gtaaggcgac tggctatccg atcgctaagc tagctgctaa
    33601 aatagcgctt ggattaacat tagacgaaat gaaaaatcct gttacaggaa cgacttttgc
    33661 agaatttgaa cctgcattag attatgttgt agctaaaatt cctcgctggc cgtttgataa
    33721 gtttgaaaac ggagaacggc ttttaggcac gcagatgaaa gcaactggtg aagtgatggc
    33781 tatcggtcgg aatatcgaag agtctttatt gaaagctgtg cgttctttag aaatcggtac
    33841 acaccatatc gaacttcctg agttggcaga catcacggac gatgacctaa tccaaaaagt
    33901 ggttcgagca caagatgatc gcctgtttta tgtagctgaa gctattagaa gagggtacac
    33961 catagaagaa ttagcggatt tgacaaaaat cgatttattc tttttagata aactgttgca
    34021 tatcgtagaa atcgaacagc agttgaaaca gcagccgttg gatgacaaga tattgaaaac
    34081 agcgaaacaa aacggtttta cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga
    34141 agccgttcgc cagatgagaa aagaaaatga tttgattcct gtatataaga tggttgacac
    34201 ttgtgcagcg gaattcgaat caaaaacacc ttatttttat agttcctatg aatatgaaaa
    34261 tgaaagttca cgatcagaaa agccctcaat tctagtactg ggatctggtc ctatccgtat
    34321 cggacaaggg gttgaatttg actatgcgac tgtgcattct gtcaaagcaa tccagcaggc
    34381 aggatacgaa gcaatcatca tgaacagcaa tcctgaaaca gtttcgactg acttttctat
    34441 ttcggataaa ttatattttg agccattgac cttcgaagat gtgatgaatg tgatcgagct
    34501 agaacagccg ataggagtaa tcgttcaatt tggtggtcag acagcaatca acttagccga
    34561 acctttgact gctgctggtg taaaaatctt agggacaacg atcgaagact tggatcgtgc
    34621 ggaaaatcgt gatttattcg agcaagcgct gaaagagttg gatattcctc aacctttagg
    34681 ggatactgct acaagtaaag aagaagccgt ggttatcgct tcgagaattg gttatccagt
    34741 tcttgttcgt ccaagctatg tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg
    34801 agatttagaa gattacatgg aacatgctgt gaaagcatct ccagaacatc cagtactagt
    34861 ggaccgttac ttgcttggtg gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt
    34921 actcatccca gggatcatgg agcatattga gcgtgctggt gtccattcag gggattcaat
    34981 ggctgtgtat ccacctcaaa cattatctga atcgatcaag aaaacgatcg aagattacac
    35041 gatccgctta gcattaggtt tgaattgtgt cgggatgatg aatatccagt ttgtcatcca
    35101 tgaaaatgag gtttatgtga tcgaagtcaa tcctcgagca agtcggactg ttccattctt
    35161 aagtaaggtg acaggaattc caatggcaca aattgcgacg aaagctattt tgggagagaa
    35221 actaaagaca cttggttatg aaaacggact ttatccagaa acaaaagaag ttcatgttaa
    35281 agcacctgtc ttttctttta cgaaattgca gaaagttgat acttacttag ggcctgaaat
    35341 gaaatcgact ggagaagtaa tgggatctga cagacaatta gacaaagctt tgtacaaggc
    35401 attcgaagct tctggattac acttgcctga atatggagcc gtattattta cgattgcgga
    35461 cgaaacaaaa gaagaagcac tagaattagc taaacgtttt acagaaattg gctatagtct
    35521 gataggaacg aaacatacag ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt
    35581 agcgaaaatt tctgaggaag cagcagaaaa aaatgtagtc gacttaataa gagatggtaa
    35641 aacacaagtg gttgtcaata cgattgataa agatcgggga aatgcttcta aagacggttt
    35701 catcatccga agagaagcag ttgaacatgg tattccattg tttacttctt tagacacagc
    35761 agatgcgatc gttcgagtga tggaatcccg tgctttttca actaacccta tttaacggcg
    35821 agcacatgtt aagacaaaaa gacgaaaaac cgttgcagga ggggacagat gaaacaagaa
    35881 atgatggaaa tcgttcagca aaaacagctg gcaccaaaga tttttgaaat gacattgaaa
    35941 ggtgatctag taaaagaaat gcagcaacca ggacagttct tgcatattct cgtgccgcgt
    36001 gcagacttgc tattacgccg accgattagc ttgaatcata tcgaccacga aacaaatact
    36061 tgccgtatca tttatcgggt ggaaggtgag gggactagag tcttctctac tctttctgcc
    36121 ggagaccatt tagatgtcat gggtccttta ggcaatggtt ttgatttgtc caatcttcat
    36181 acaggagacg aagtatttat aatcggtgga ggaatcggtg tccctcctct atatgagtta
    36241 tcaaaacagt tagtcgaaaa aggaattaag ccaacgcatt ttttaggttt tgccacacat
    36301 gaagtcgttt attatgaaaa agaatttaat cgtctaggag aaacaagaat cgctactgac
    36361 gatggaacat acggtatgca cggaaatgtc ggtaatttat tgttaggaac gaagacggaa
    36421 cctacagctg tttttgcatg tggttcaaac gggttattga agacagtgga acagctgttt
    36481 tcttcgcatc aaaatgtcca gttatcatta gaatcccgca tggcatgtgg gataggggct
    36541 tgctatgctt gcgtttgtca tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt
    36601 tgtgacgaag gaccgatttt taaaatagga gaggtaatta tatgaatgag aatcctttag
    36661 cggtcaaact tcctggccta gaattaaaaa atcctatcat gccggcaagc ggctgtttcg
    36721 gttttggcaa agaatacgga aaatactatg atttgaatca gcttggatcg atcatggtca
    36781 aggcaacgac tccaaatgcc cgctttggca atcccacgcc aagagtggcg gaaacaccat
    36841 ctggcatgtt gaatgcaatt ggtttgcaaa atcctggtct agacgttgtt atgcataaac
    36901 ttcttccaga attagaggag tacaatgagt tacccatcat cgcaaatgtt gctggtgctt
    36961 gtgaagaaga ctatgtagaa gtttgtagca aaattggcga agcaccaaat gtgaaagcaa
    37021 tcgagctgaa catttcttgc ccaaatgtca aacatggcgg aattgctttt ggaacagatt
    37081 ctgatgttgc ttttcaatta acacaagcag taaagaaagt ttcttctgtt cctgtctatg
    37141 tcaaattgtc cccaaatgta acagacatcg ttccaatcgc tcaggcaatc gaagctggag
    37201 gggcagatgg gtttacgatg atcaatactc ttcttggtat gcggattgac cttaagacaa
    37261 gaaaaccgat tttagccaat caaacaggtg gactctccgg accagcaatc aagcctgtcg
    37321 ctattcgttt gattcaccaa gtagcaagta tatccagcct tccaatcatt gggatgggcg
    37381 gcgtgcagac agtggatgat gttcttgaga tgtttatggc tggagcaagt gctgtagcta
    37441 tagggaccgc gaatttcacc gatccgtata tctgtcctaa actaattaaa gagctccctg
    37501 tgcgaatgag cgaactaggc atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg
    37561 aacgtgaacg atgaatcaac tagaccaacg accaattatt gcattagatt tttcaactag
    37621 gcaagaggta gaagactttc ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat
    37681 cggaatggaa ttgttctacc aagaaggacc tgaaatcgta cgttatctaa aagaggctgg
    37741 tcataatgta tttttagatc tgaaacttca cgatattccg aatacggtcg aaaaagcaat
    37801 gcggggattg gcaaagcttg gtgttgatct cacctgtgtt catgccgctg gcggtattcg
    37861 tatgatggag gcggcaatgc gaggattaga agaggggact ccagaaggtg gaaaacgtcc
    37921 gctacttcta gctatcactc agcttacttc tacaagtgaa gaagaaatgc atgccgatca
    37981 actaatcgaa gtttctttag aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc
    38041 aggattagat ggtgtggttt cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg
    38101 agacgaattt gtttgcttaa ctccgggtat aagaccagaa ggaacagtcg caggagatca
    38161 gacaagagta gtcacacctt ctcaggcaaa aaagatcggc tcaactttta tcgttatagg
    38221 tcgaccaatc actcagtcaa ctaatcctta taaagcatat caaacgattc aaacagaatg
    38281 gagctaacct aaaatgaatg ttgaacaaag tatcgcaaaa gacttattag aaattgaagc
    38341 agtattttta aatccaagta atccttttac atgggcaagt ggaatcaaaa gcccaattta
    38401 ctgtgataac cgaatcacga tgagctatcc aaaagtccga aaagagatcg caaaaggatt
    38461 agcaagcaaa atcaaagaag cttaccctga agtacaagtg attgctggaa ctgcaacagc
    38521 aggaatccct catgccgcat gggtagcgga aatactggat ttgccaatgg tctatattcg
    38581 aagtaaggcg aaagatcatg ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca
    38641 gaagatggtt gtgatcgagg atttgatttc tacaggcgga agtgtcttgg aagcagctga
    38701 agcagcaaaa agagaaggtg cggatatcct tggtgttgcg gctatcttta cgtatgagtt
    38761 gccaaaagga aaagcaaatt tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta
    38821 ttctgtattg attgaagcag ctttagaaga tcgttatatc gatgagcaag aattaactct
    38881 gctaaaagaa tggaaaaaag atcctgaaaa ttggcagatt gaagcgcgct tgatgaagat
    38941 agagaaatcc gatttgtgtc tccagaatca tcaattggaa tagcaaaaat ccaaaaactg
    39001 attgcgagaa gcatcttttc atcaggtatg aaaagaaact tctatcgtga tcagtttttg
    39061 atttttttaa aattcatttt tctaagactg gttagcagaa agaagagaat aatctcttct
    39121 cttttttcta ttatatatag attttctcta ttttttggta agggagctgt ttcaaaaatg
    39181 gtaaagagcg gaaatgttgc gaatatcagt tatttaatga tatgatattc ggaaaaagaa
    39241 atctcaaagg gaggtaactt ttatggggtt gaatgaattt attttaggat tgaacaattt
    39301 agagacgatc acaagggcac ctggattttt taaatataca gagcatacag ttgcagcaca
    39361 ttcgtatcgt gtagcatcta ttgcgcaagt tctaggagac attgaagaag cgaatggaca
    39421 accgatcgat tggaaactgc tctatgaaaa atcattgaat catgactaca cagagcgttt
    39481 tattggtgat atcaaaacgc cggtgaaata cgcaagcaaa gagttaagag ggatgctgca
    39541 aacagttgaa gaaaagatga cggacgaatt catcaaccaa gagattccag ctgagttcca
    39601 agaagtttat cgccgtcggt tgttcgaagg aaaagatgaa accgtagaag gagaactatt
    39661 agccatttca gataaggttg accttcttta tgaatctttt gatgaaatca tcaaaaataa
    39721 cccggaaaaa gtttataagg agatgttcat tgaggcagtg actacgatta aaagctttga
    39781 acatcggcct tctgttcagt atttctttaa agagatattt ccggaactat tgaaccagga
    39841 attttatgga aaagaggcgt ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa
    39901 ataaaaatga tatgagatgc agggggaagt atagttgaaa aagaatatgg atgtcgaatg
    39961 gggctattct ggagacaaag gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag
    40021 aggtgcggaa tttccattgc agtcgcctat tcatttgacc aagggggaag aaaccaaaat
    40081 agaaggagac acgctttcct ttcattatca aaagcaacgg tttactgaca aggaattcaa
    40141 aaacacgatt catttagtac cttttgacca actcagttat gtagagttca agaaagaacg
    40201 ctattatttg acagatattc atttccactt gccttctgaa catatcatca acggaaaaca
    40261 ggaagatatt gaatttcatt ttgttcatat gaacagcaaa aaagagaatc tagttgtggg
    40321 tgtcatgtac cagctaatgg tacaggaagg ttggctgtat aatcaagaaa atggggaaag
    40381 ttggaactta gaaaaacatg agcattgggt aaatccggat gtgttctttc cggagcaaaa
    40441 aagccatttc cattatattg gctcattgac aactccgcct accagcgggc caatccaatg
    40501 gtttgtaatg gatcatgtag gaaaactgaa ccaagagttt cttctcccct tcgatggtca
    40561 gtatgctcga ccgaacaacc gaccaatcca gcccttgaat ggaagagaaa tttattattt
    40621 cgaggagttt gagcaatgaa tattcagcag atgaaatacg tggtcgctgt agcaaacaat
    40681 ggtagtttca gagaagcggc aaagaaaatg tttgtctccc agccaagtct ttcagctggt
    40741 atcaaagaat tagaaaccga gttagggatc acgctgttta ctcgaacgaa tcgaggggca
    40801 tatttaacag aagaagggca agacttctta aatcgtgccg agaaaatatt ggtccagctt
    40861 gatacattag aaactcacta tctaacgagt gaaaagagag acaatttttc gatcgctgcc
    40921 cagcattatg atttcttagg tcctttgacc gcacagctac tcgaggaatt caaagacgag
    40981 atcaaacaat ttcggatcat ggagacgaca acagcgagag tgatcgaaga agtcaaagag
    41041 cagcacagtg agatcggcat tatttatatg aatgagcata atcaagctgg tatcagaaga
    41101 tatttacaac aaggagagct aagtggccgc agcattggaa aattcaaaac gcatattttt
    41161 ttaaggcaag gacatccatt agctgaacac gcagagatca ccaaagaaga attgttatct
    41221 tatcctcaag ttcgttttac acaggatgga aataatttcc cgtattttta tgaagacctt
    41281 atcgagatcc ctgatcaaga aacagtgatt tatacgagtg atcgaggtac tttgatgaat
    41341 atcgttcttg aaactgatgc ttacgcttca ggatctggta ttgtgattgg agggattcgc
    41401 gagcacttgc ggttaatccc tttggcagaa ggcgaactaa atgaatttta tatcatttat
    41461 tcggcgaaac gtccgctcag tgaaatcgct caaagattta tcaaagagct tactcggtta
    41521 ctggaccagc agagctaaga aaagcagatg agaacgctgt ttattgaaca ccgttgatca
    41581 aaaaagaggg gttgtgacag aattttgtca caactcctct ttttgtagca aacaatttat
    41641 aactaattgc tgttgtggga aagtacgcaa atatgattct agaaacactc attgattacc
    41701 tgttttgttt catagctgta attttttctt cttctggtgt cacgataatc gtcttttggt
    41761 tttcataaat aacataacca ggcttggcgc catttggttt tcggatatgc tttacctgta
    41821 caagatcaac cggcacttgg gcagagtatc ggtattttga aaaataggca gctaactcgg
    41881 cagcttctgt aattgtttca tctgagggct tgtcactttt aatgatgacg tgcgaaccgg
    41941 gtatattttt tgcatgaagc caatagtcgg tttttttagc tgtcttcatt gtcagctggt
    42001 cattttgaag attgttttta ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat
    42061 caggctgaga tggtttctta cgtttttgtt tttttaagtt cttctttttc aggtatcctt
    42121 ctgaagtcaa ttcttcttta attgcttcga tatccattgg gccggcaatt tccagttgag
    42181 ataagacaga ttcaagatat tggatttcat ctttggcttc ttggatttgt tcgccaatca
    42241 gcttaacagc gttcttcagc ttttggtatc gatggaaata tttctgtgca ttttgatttg
    42301 gtgtcaatgc cggatctagt gctattttga ttggccgatc ttcttcataa taattaggta
    42361 aaacaacttc ttttgcgccg cgtggtactt gtgtcagaaa agtagtaagt agttcgccat
    42421 ttctacggta attctcagcg ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt
    42481 tatttttatt tcgttttagt tcattttcga tttttcgaat cagttctccg ccttgttgtt
    42541 ttgcccgatc tttctctgct ttttcgtgat aatagctatc taataattga ctgagtgttg
    42601 gataagctgt tacagcagat gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa
    42661 aatctttatt ttccgtttcg taaaaagtag gaataggctg atggctgatg gctgagaaaa
    42721 attcgtccca tacaaccatc ttttcattag gacgttccag taatcttttg gtcagctctt
    42781 gagcgatatc gaaaccgatt ccctgaaact gacgttggat tgcttttgga tctagttcag
    42841 tttgggaaag ccgctgaaag attttttctt tctcggagct aaatgggttc aagacctctt
    42901 gttttggcgg agcaacatat tctacaccag gaagcaaaga acgataagtg ttttgagagc
    42961 ttccaatgtg tttgatcgca tccagtattt ttccagtttc tcgattaact aagacgatcg
    43021 tactatgacg tcccattaat tcaacgatca atatgatatt ttgtagatca cccaactcgt
    43081 ttcgtttagc aaagtggaaa tggatcactc gatcattttc gatttgttcg atgtcttcta
    43141 aaatagcgcc atccaaatat tttcgaagca tcatcacgaa gttaggcgga ttatcaggat
    43201 tctgataatc gatttgggta atctgtacac gagcataact tgggtgcgca gataataata
    43261 atcgatggtt tttgccgcgt gaacgaatca ccagcacgat ttcattttca taaggctgat
    43321 ggattttcga gatccggcca gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg
    43381 taaatacacc atcaaaggac atggttcatt cctcatttct tttcataagt atattcatta
    43441 taacgaaaaa aagaagaaaa accaatttgg ccgaatagta aagcaaaagt ctagaaaaat
    43501 cttggactaa ttacataaaa aacagtgttt ctatcgttga aatccaaatt tttaggt
//
LOCUS       DARDHD010000008        41025 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000008 DARDHD010000000
VERSION     DARDHD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.008"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SDA06_000359"
     CDS             complement(38..1189)
                     /locus_tag="SDA06_000359"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG4227656.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SDA06_000360"
     CDS             complement(1202..1639)
                     /locus_tag="SDA06_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4227657.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SDA06_000361"
     CDS             complement(1654..3420)
                     /locus_tag="SDA06_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG4227658.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SDA06_000362"
     CDS             3684..4361
                     /locus_tag="SDA06_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227659.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SDA06_000363"
     CDS             4364..5491
                     /locus_tag="SDA06_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227660.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SDA06_000364"
     CDS             complement(5691..6131)
                     /locus_tag="SDA06_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4227661.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SDA06_000365"
     CDS             complement(6142..8175)
                     /locus_tag="SDA06_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4227662.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SDA06_000366"
     CDS             complement(8177..9880)
                     /locus_tag="SDA06_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4227663.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SDA06_000367"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SDA06_000367"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG4227664.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SDA06_000368"
     CDS             complement(10963..11988)
                     /locus_tag="SDA06_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4227665.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SDA06_000369"
     CDS             complement(12197..13066)
                     /locus_tag="SDA06_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG4227666.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SDA06_000370"
     CDS             complement(13525..15417)
                     /locus_tag="SDA06_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227667.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SDA06_000371"
     CDS             complement(15414..17141)
                     /locus_tag="SDA06_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227668.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SDA06_000372"
     CDS             complement(17157..17807)
                     /locus_tag="SDA06_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4227669.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SDA06_000373"
     CDS             18011..18307
                     /locus_tag="SDA06_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4227670.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SDA06_000374"
     CDS             18479..19099
                     /locus_tag="SDA06_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG4227671.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SDA06_000375"
     CDS             complement(19191..20357)
                     /locus_tag="SDA06_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4227672.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SDA06_000376"
     CDS             complement(20369..20722)
                     /locus_tag="SDA06_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4227673.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SDA06_000377"
     CDS             20850..21872
                     /locus_tag="SDA06_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG4227674.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SDA06_000378"
     CDS             21891..22052
                     /locus_tag="SDA06_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227675.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SDA06_000379"
     CDS             complement(22311..22745)
                     /locus_tag="SDA06_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227676.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SDA06_000380"
     CDS             complement(22855..24714)
                     /locus_tag="SDA06_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4227677.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SDA06_000381"
     CDS             complement(24832..25992)
                     /locus_tag="SDA06_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG4227678.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SDA06_000382"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SDA06_000382"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG4227679.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SDA06_000383"
     CDS             complement(27739..28437)
                     /locus_tag="SDA06_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4227680.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SDA06_000384"
     CDS             complement(28394..30055)
                     /locus_tag="SDA06_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG4227681.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SDA06_000385"
     CDS             30273..31673
                     /locus_tag="SDA06_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG4227682.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SDA06_000386"
     CDS             complement(31925..33004)
                     /locus_tag="SDA06_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4227683.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SDA06_000387"
     CDS             complement(33090..34049)
                     /locus_tag="SDA06_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4227684.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SDA06_000388"
     CDS             complement(34061..35572)
                     /locus_tag="SDA06_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4227685.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SDA06_000389"
     CDS             complement(35594..36157)
                     /locus_tag="SDA06_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG4227686.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SDA06_000390"
     CDS             complement(36163..37026)
                     /locus_tag="SDA06_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4227687.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SDA06_000391"
     CDS             complement(37040..37936)
                     /locus_tag="SDA06_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4227688.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SDA06_000392"
     CDS             complement(38111..39400)
                     /locus_tag="SDA06_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4227689.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SDA06_000393"
     CDS             complement(39372..40889)
                     /locus_tag="SDA06_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4227690.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11836 a         8590 c         7097 g        13502 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata attta
//
LOCUS       DARDHD010000009        34295 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000009 DARDHD010000000
VERSION     DARDHD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.009"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            147..1373
                     /locus_tag="SDA06_000394"
     CDS             147..1373
                     /locus_tag="SDA06_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4227691.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1461..1853)
                     /gene="rpsI"
                     /locus_tag="SDA06_000395"
     CDS             complement(1461..1853)
                     /gene="rpsI"
                     /locus_tag="SDA06_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4227692.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1869..2312)
                     /gene="rplM"
                     /locus_tag="SDA06_000396"
     CDS             complement(1869..2312)
                     /gene="rplM"
                     /locus_tag="SDA06_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG4227693.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2831..3298)
                     /locus_tag="SDA06_000397"
     CDS             complement(2831..3298)
                     /locus_tag="SDA06_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG4227694.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3418..4956)
                     /locus_tag="SDA06_000398"
     CDS             complement(3418..4956)
                     /locus_tag="SDA06_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG4227695.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5036..5722
                     /locus_tag="SDA06_000399"
     CDS             5036..5722
                     /locus_tag="SDA06_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG4227696.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5923..6450)
                     /gene="msrA"
                     /locus_tag="SDA06_000400"
     CDS             complement(5923..6450)
                     /gene="msrA"
                     /locus_tag="SDA06_000400"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4227697.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6534..7211)
                     /gene="pgmB"
                     /locus_tag="SDA06_000401"
     CDS             complement(6534..7211)
                     /gene="pgmB"
                     /locus_tag="SDA06_000401"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4227698.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7208..8338)
                     /locus_tag="SDA06_000402"
     CDS             complement(7208..8338)
                     /locus_tag="SDA06_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4227699.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8338..10521)
                     /locus_tag="SDA06_000403"
     CDS             complement(8338..10521)
                     /locus_tag="SDA06_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4227700.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10518..11543)
                     /locus_tag="SDA06_000404"
     CDS             complement(10518..11543)
                     /locus_tag="SDA06_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4227701.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11588..12415)
                     /locus_tag="SDA06_000405"
     CDS             complement(11588..12415)
                     /locus_tag="SDA06_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4227702.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12412..13479)
                     /locus_tag="SDA06_000406"
     CDS             complement(12412..13479)
                     /locus_tag="SDA06_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG4227703.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13505..14341)
                     /locus_tag="SDA06_000407"
     CDS             complement(13505..14341)
                     /locus_tag="SDA06_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4227704.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14338..15207)
                     /locus_tag="SDA06_000408"
     CDS             complement(14338..15207)
                     /locus_tag="SDA06_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4227705.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15277..16560)
                     /locus_tag="SDA06_000409"
     CDS             complement(15277..16560)
                     /locus_tag="SDA06_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4227706.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16574..18259)
                     /locus_tag="SDA06_000410"
     CDS             complement(16574..18259)
                     /locus_tag="SDA06_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG4227707.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18534..19559
                     /locus_tag="SDA06_000411"
     CDS             18534..19559
                     /locus_tag="SDA06_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4227708.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20154..23828)
                     /gene="rpoC"
                     /locus_tag="SDA06_000412"
     CDS             complement(20154..23828)
                     /gene="rpoC"
                     /locus_tag="SDA06_000412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG4227709.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKTETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23886..27503)
                     /gene="rpoB"
                     /locus_tag="SDA06_000413"
     CDS             complement(23886..27503)
                     /gene="rpoB"
                     /locus_tag="SDA06_000413"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4227710.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28231..29199
                     /locus_tag="SDA06_000414"
     CDS             28231..29199
                     /locus_tag="SDA06_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4227711.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29217..30122
                     /locus_tag="SDA06_000415"
     CDS             29217..30122
                     /locus_tag="SDA06_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227712.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30119..30925
                     /locus_tag="SDA06_000416"
     CDS             30119..30925
                     /locus_tag="SDA06_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227713.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31061..32050
                     /locus_tag="SDA06_000417"
     CDS             31061..32050
                     /locus_tag="SDA06_000417"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG4227714.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32367..32891)
                     /locus_tag="SDA06_000418"
     CDS             complement(32367..32891)
                     /locus_tag="SDA06_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG4227715.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33178..>34295
                     /gene="scm"
                     /locus_tag="SDA06_000419"
     CDS             33178..>34295
                     /gene="scm"
                     /locus_tag="SDA06_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4227716.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT         9794 a         7279 c         6422 g        10800 t
ORIGIN      
        1 ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt tatgaataca
       61 tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga aatatactgc
      121 cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg aaatataaaa
      181 ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca gaaaagtatc
      241 aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg aagaagaaaa
      301 tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga aaaataacgt
      361 ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa acaattagat
      421 caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt cggttgcata
      481 ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact gagataatat
      541 cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata aaaggatatg
      601 ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat cgaactacta
      661 aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa agaatacaag
      721 aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca gtaaatgatg
      781 attttaataa tcatttactt acttttcatg attacacact ttttatgatt accctgtatc
      841 ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat tttgaaaaac
      901 aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt acaaaaggtc
      961 gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt gaatggaaat
     1021 cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat cagtatctct
     1081 ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat tacctaaact
     1141 atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct ccacacaaac
     1201 tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat caaatttcaa
     1261 atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca ccagatatag
     1321 ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat tgatatgtgt
     1381 tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa agtgccagaa
     1441 acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg tgctttctta
     1501 agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc agcacgttta
     1561 agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc atgacggata
     1621 gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc atatgcgcct
     1681 tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc atgtggaatg
     1741 tattcttcaa catctttttt atttacagta attttgccag ttcctggtac tagacgtacg
     1801 cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc caatgaaatt
     1861 ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc tgcatgtgga
     1921 tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa agtgttttta
     1981 ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc acgcaattcg
     2041 ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata aattttgtca
     2101 gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac gaaatcgcca
     2161 gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga tgcaacaact
     2221 gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt acgttctact
     2281 tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt aagttctgat
     2341 gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac catttaacat
     2401 attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa tttatatgaa
     2461 actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa tgaaaaacaa
     2521 aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg gtgttctcct
     2581 gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt tttttaaaat
     2641 tgtttcttgt gactcggagc tgaacggcag gcttacaacc tcttggtaaa cggtgttcaa
     2701 tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgacgtt
     2761 tgtttctcta atactcaggt caaaacgctg atttcacaac ctctttttcg gtcaagttca
     2821 acaacagttt ttacgcgtcg atctctggcg ctttgcccaa atgagcttgg atcatccaga
     2881 tacgtttttc tgtggctgtt ttgaattcga taaagatatc ttgtgttgga tcgtcgcctt
     2941 cttctccaga aacttcgatt cctttttggt aaagatctgc tagataacga tagcctgcaa
     3001 caagtgtttc caaacgttct ggaatagtac gatcccagcg gccaatttca tcagggattt
     3061 ttgtgttatc tgcaaattca cgcaatgtag aataaggtga accgtctaat gtgatcaagc
     3121 gttcggatac ttcatctaac tgagcgttca aatcatccat gaactcatcc atcattgggt
     3181 gcaatgtaag gaaacctggt cctctcatgt accaatgtgt ttggtgaaca acaacagaca
     3241 tttggcttag atctgcaact aattgattta atacttcttt ggtttgttgg aatttcatct
     3301 ttcttgcctc ctaacatttt cttacactta aaataatagt agactttcaa tgaaaagtac
     3361 aacaaaatca tttacctgag aacgattttc agttcgcttt tttatccgta aagccaatca
     3421 tttttcagaa tcagctaatt cttctcgcag ttgagcaaat tgctgataag tctgtgctaa
     3481 atgcttttgt gtcgaaacaa ataatggata gattttctga tattttttat gatcagtttc
     3541 ttgtactcca acttgctctt tcaattctat caattcctgt gcttcactga tactttggat
     3601 ctttccgatg cttctccagc ctaaaatcac agctcctaat gcggatgctt ctgtgacagt
     3661 agggaaaaca agcgggtaac ccagtacatc agccatcatc tgacgaaaaa tcggagattg
     3721 agcaaacccg cctgtagcac ggatctctgt gagctgattt tcctggcaaa ctccttttaa
     3781 aatccgataa aggttgagac agatcccttc tagaatcgcc ttcatcattt ctttttctgt
     3841 atgttgccgc tgtaaaccga taaaactgcc aaacgcatct gcgtcccaaa gcggtgctcg
     3901 ctcaccaagt aaatagggat gaaaaatcag accattggca cccgctggca caaaagcgat
     3961 agccgaaagc agtgcatcat atggattttc tcctttttct ttagcagatt gaacgacttc
     4021 ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca ccattgctag tcgctcctcc
     4081 cactacccaa tgtttctgat ccagaacata acaaaacgtc tccatttctg gatggaactg
     4141 aggctgagta gtaataaaac gaagtgctcc gctcgttcct acggtcaaag cggcgatcgt
     4201 atcaccaaaa gcacctaacc ctagattaga aagcggcccg tctgcccctc ccaaaacaaa
     4261 tgatatatca tttggcaaac ctagttctgc tgctgttttt gatggtagac ctgttacttg
     4321 ataggtggaa ggaaagactt gcggcaattg ctctattcga atgttcaagt agtcaagcac
     4381 ttcttctgcc cattggaatt catggatatc aaaatatcca gttcctgaag ctgttccgta
     4441 gtcgctgatc agttcgccaa acaatctata aaagatatag tctttgatac cgacaaaata
     4501 agcagttttt ttgaataatt caggttggct gttttgcagc cacttgattt tgttcaatgg
     4561 tgtcattgga tgaatcggcg tcccagtctg ccgataaaat tctttgccaa gcacggtata
     4621 tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc caagtgatca ttcgagataa
     4681 cggtgttttc tcttcatcca acaatatcag gccatgcatc gcagaggaga aggagacaaa
     4741 ttgaatgatt gtgtctgaag gcaactgttt aattacttct tttataacgg tgcagacacc
     4801 ttgaaagatc aattcctgat cttgttctgc catcccttgt gtttcttgga tcaaagggta
     4861 gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg aaagcaactg ctttggtttg
     4921 tgtggttccg atatctaatc cgattgaaac gttcattttt tcacctcaaa ttttatgtat
     4981 gtacaaaaat ttagtttaag cttgcgtatc ttggttttga aaagggggag atggcgtgtt
     5041 actttatttt atcattggtt gctgtatcgg atcattttta tgtttaaccg cccagcggat
     5101 tccgttaggt cactctatta tttatcctag atctcattgt gtcaaatgct gtcgttcgct
     5161 ttcttggtat gaattgattc cgattctctc gatcatcgtc caacgatttc gctgccgcta
     5221 ttgcaggtgt cggttgccct tctactattt gttggcagaa gccttatgtg gtggtctttt
     5281 tgcctggttc tttacttttt cttccgcaag aaatttttcg acgttcatat ggatgctttc
     5341 ggctttgcta ttttcgttga tggatttatt ttacttcatg gtccattctc acacgctatt
     5401 ctgttcttgg gcaattttat ggattttttg gttgcaggcc ggcgtcttcc aatggcaaag
     5461 tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc tatggtcaat cctatctcgg
     5521 agctggcgat actctgttga tgctttcttg gagtggtggc ttgtctctag aagagttgct
     5581 tcagatcttg tttcttgcta gtctgtttgg aatatccgtt ttttctgttt actttatctg
     5641 tcataaaaaa aagttggaaa agctgccttt cattcctttt ttgagcagtg cgttgctgat
     5701 tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac gacttgtcac aacctctgat
     5761 atatagtgtt ctcttaccaa atccttgtga cttggagcta ggcgacaggt tcacaacctc
     5821 tccactaaat ggtgttcaat cagctgaccc tcagtattag ctcaaaatgc tggttttaca
     5881 acctctgata tacggtgttc cataagtagc tttttcggct tcttatttct tgacgcccca
     5941 atgtcgacga aggaaacctg cacgtccaga accttctcga taaagattgt aatggagctt
     6001 gtttttcttg taataatcct gatggtaatc ttctgcagga taaaattcgc ttcttggttc
     6061 gatttttgtc acgattggct tatcgaatcg cccgctgtct tgcaatgctt gttttgaacg
     6121 ctctgcaata tttcgttctt cttcatcatt atagtagatc actggacgat aagaatcacc
     6181 gcgatcagcg aactgtccta atgcgtcagt cggatcggtt tgctgccaat agatttccac
     6241 caactgatca taagaaataa cttctggatc atattcgatc tctactgctt ctgtatgccc
     6301 tgttgtgcct gttgttactt gttcataagt tggatttggt acatgcccgc cagtataacc
     6361 ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt ttgaccatac accaaaagca
     6421 tccgcctgcg aaaactgctt tttttgtcat ataaaatccc ccttttttct catcatacct
     6481 tgatttccaa aaggaggata acgttttgct tgtgacagaa aaaacggaaa ctattatata
     6541 cgtgatacgg cttctgataa atgccaaaac gaacgtactt gatcaaaaac ggttaaatca
     6601 ggaatcacat attcagcaga aggaagattt atgttttcac ctattcccaa tacatgcatg
     6661 ttaccagcca aagctgcttg acaaccagct tctgaatctt caatgaccaa gcatgcattt
     6721 ggcggtacat tcaattgctg tgctcctttg agaaaaactt ctggatcagg ttttgctttt
     6781 gatacttgcg tgccgtcaat aaggacatca aaataacttg tcaaattcgt tttttctaaa
     6841 atgaggcgtg catttttgct tgctgatccc aatccgatct tgatgtttgt ttttttcaag
     6901 tactcaagaa cagctattgc accaggcaaa acggatgttt catccatttt ttgaatcgct
     6961 tgaagatata gattgttttt ttgctcagca aaggctgatt tctctttttc tgagaatttc
     7021 tgttccattt ttccaaatga cagcaatcgc tctagtgact cggttctgga tattcctttc
     7081 agttgctcgt tttctttctc agtgaatgga atggacagct ggttagctaa aaccagccac
     7141 gcttcgtaat gataacgagc tgtgtctaca agaacgccat ctaaatcgaa tagtgctgct
     7201 ttgattttca tcgattacct ccttgaaaat gaacgacttg aagtaaagat gcgatgtttt
     7261 tagctgtgcg atgaagatta tcagtggctt gctgtaaagc ttcttgtaaa ggcacagctg
     7321 caggaaggat cggaaaaacg gccgaaatgc caacatctgc tgctttttcg cttcctttac
     7381 caacacttcc gcaaatagct atcacaggaa cgccctctgg tactcttcta gcaatcccaa
     7441 ctggcgcttt tccttttaga ctttgtccat ctagacgtcc ttcgcctacg attacgaggt
     7501 ctgcttgctg tacccgttta tcgaactcca gtaaatccaa tacgtagtcg atgccagaga
     7561 ctattttccc tgaagcaaat gctaacagtc ctgcacccat tccaccacca gcactactgc
     7621 ccgatgcctc gattacttga ggggcaaatc tcatataaaa atcatgcatg gcttggtcag
     7681 ccactgctaa ttcattttct ttcaagcctt tttgtggtcc gaaaacaaaa gaagcgcctt
     7741 cttttccgca taaaagatta gtaacatcag taatgacagt gattttggct ttaatcagct
     7801 caataggcac gttcgtttta tcaactgtag caattttacc tagattttct cccagtgctt
     7861 ctactttttg attatcttga tcgtaaaaag cataacctag accttctgcc atgccgatgc
     7921 cgccatcgtt ggtactactt ccgccaacgc cgatgatgat gtgtcgaaca ccttgttgca
     7981 acaattgtcg tatcagttct cctactcctc gcgtggataa cctaagtggc tgttgttggt
     8041 ttagtgggac atctttcaat ccgacgattt ctgccatttc aaaaatcgca gtttgaccgt
     8101 ctgaagcgta ctggatttct ttgtttttgc caaatgcatt agtaacgact gctgtttgcc
     8161 gcttcaacct taaactgtct gctaatgatg aaaccgttcc ttctcctcca tcgcctatag
     8221 gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa acctgattga atggcttgtg
     8281 ctgcctctaa ggctgtcaga ctttctttga aagaatctgg cgcaatcaga atacgcatta
     8341 ttttcccctc cgttccaaga tcagttcttt ttccagctgg tattggtctc cattgacaaa
     8401 aataggtaat gtttctttcg tctctttttt tattactatt tgtttttttg tcaaaacaaa
     8461 tagcaacctg tgatttttcc aaataaaagg aaaagaaatt ttttcccatg ctgaaggaag
     8521 tcttggagaa atagaaagag ttccattcgt tactgtaata cctgaaaaac caaatataac
     8581 tgctagccat agtgctccta atgaagcagc atgaattccg tcatctgatg aatgcggatc
     8641 tggtcctaaa tcaatcaggc acgcttcttg gaataaatga tacgcccatt ccatttcttg
     8701 gatacggcaa gcctctatgg cgtgaatcgc tttactcaac gatgagtcgt ggattgtttt
     8761 gttttcatag taatgcaaat tcttagaaac gattggttga gaaaaatact caggtaataa
     8821 gtataacagc atgacaagat ctgcttgctt gctgacttgg agttcattta cttcgctacg
     8881 ggcataatca agtaaaatag actggctgcc aggacgttct ttgtaagtgg tcaaatcaat
     8941 aactggctta ctgagatacg tgtcatcttg cggaatcagc tgttctttgt taggcactgg
     9001 caaatataac ttatctaaaa aattttgaca ttctttcaaa aaaagcggat cgccagcttg
     9061 atatttaaca gctaaggcca cattataatg cgccagatag ttcgtatatg cattattgtc
     9121 gatatattct gtgtattcat ctggaccaat gactctgtgt atctctaaat gatcatcgac
     9181 ttttacagct ctgctcagcc aaaaagctgc tgtttcatga atgagcgttt ttccctttgt
     9241 ttctaaaaac gattgatcct ctgttgcttg gacatactct tgaacggcat aagcaaggtc
     9301 tgcactgata tgatgttctg cggcagctga agcaacttct tgtcttgttc cggttttgat
     9361 attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg tgtgcgcttt cccatgggaa
     9421 aagtgcacct tgatagtgat aatctcttgc tttttgccgt gcgccttcta atcgatcata
     9481 acggtacagc agcaaattct ttgctacttc aggaaactga tataaaaaga aaggcaggat
     9541 aaaaatttcc gtatcccaga aaacatgccc tttataacct tcgccagtta gtcccttcgc
     9601 ccctacacta acacttggat cattgcgggg agtcataatc tgtaaatggt aagcagcgaa
     9661 gtccatcgct aactgatcga aagaagaatc gccttggatc gttatccgat gctgatgcca
     9721 aaaatctgtc caataagaca tcgactgctc taaaatttct gtataactaa cgcttttctg
     9781 ggcttgggca atacttttct taaacgggtc tgtttgctga agagaggtag agagcacgat
     9841 gatcttctcc agcgtaaagg acgtattttt tctgatcttt tctgaaaagg aagcggtgat
     9901 ccggcgattt tttgcttgga atacacctgc tttattgaat ttagctgcaa tcccaatatc
     9961 aattttactt tctgttgtct ggtaggatgc actgatcaaa tcgtcaccaa atactcgcag
    10021 ttctttttca accaactgct gtgtaccaaa attggtttgc tgtccatcga tacccgtttt
    10081 cacatctact tggcagtctt cggtcaacgg cttgatgacc actttcaggg ctgcctgatg
    10141 gagattattt tgatcagcaa cacgacaagt aatgacttcc actttttttc cttgagcagt
    10201 ttcaatgagc gcatagcgtt taagctctcc catttctaaa tctagtgccc gctcgtatgc
    10261 aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg atttttattt cccatctgat
    10321 gacatctggg agattcacta actcggctga ttctgctcct aaaggtcggt tatatacacc
    10381 agctgcaaac attcccctta cttgattccg atagtcttcc tcctggcttc ctctgatacc
    10441 aaaaaatcca tttccaatgg tcatctgact cgcatactta ttgatatact ctaaatcaaa
    10501 ctgtttttct tctactttca taaggtaata acttctcctg tttctgccga acgatataaa
    10561 ttttccacca gattctggat gacatatcct tgccagccat ctgcaatcat ctgagtctgg
    10621 tcaccgagcg cccgattcac aaaatgagcc atacttagtt catgtgggtc attaactggc
    10681 gtggtctttt gtaaggaaac cagctttcca tctacatctt cgaatagttc gcctggatac
    10741 agattagcac ctgcctgatc gccacaaaaa gccacattca gtttgtcttc tctgatattc
    10801 aaagcaaacg aagtattcag ttggatgatc ttgccgtttt ctaattccag tgtgccaaaa
    10861 attgcatctt ctactgtgaa tttgcttgga tcccatttcc cgaatttccc tgaatttttt
    10921 ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt ttactttagg aaatccagta
    10981 atatacatcg ctgtatcaag catgtgaatg ccataatcga tcaaaggccc gcctccttga
    11041 agttctttgt cgataaacgt tccccagcct ggaacaccag accgacgaag cgcattcgct
    11101 tctacgtaat aaatatcccc gagaacatca agatgattct tgatcattgt tgcttcgtca
    11161 gaaaaacgat gctggaatcc ataagccaaa atctgcccgt ttttatcggc tgtctctgcc
    11221 atctttcttg cttgatcagc tgtcatagct ggtggtttct cgcacatcac tgaagcatta
    11281 gcttcgaggg cagccaatac atgttcgaag tgaaagcgat tgggtgtaca gacactcacg
    11341 atatcgggag aaatctcatt cagcatttgt gtaactgatg tatacgcatg agaaaatcct
    11401 gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat caaccacagc ggccaattct
    11461 aagacatctg atcttttttg ataataagca gcatgtactt tttctgctac ttgtccagcg
    11521 cctatgattg ctactttctt catttatttc tctctccttt gcttatttac ttattgttat
    11581 tctttctcta ttctttctct aaatcattta aatgacgcgc tgtttccaga cagtttttca
    11641 agtattgcaa tgattcctga taaattttca cttcattttc tcctcgcaaa cgacattcat
    11701 ataccatata accttcatac tcattttgaa gtagcgtttg aaaatggcga gtaaaatcaa
    11761 ttgcgccaga tcctggttgg tagcgatgat tatctgctaa atggatatga cccacatact
    11821 ctttttgcgt agtcaatgct tttgaaatat catcttcttc aatattcata tgataaaaat
    11881 cagcgatgat ctgcacatgt ttcaattgat tagagtcaat gtaatgacgc gcatccgcta
    11941 acaaattgat catatggtct tggtatcggt tcaaaggctc caaataaata accgtacctg
    12001 tttcttcggc tactttatct aaataacgaa gtgattcgct gactgcttta tagtctcctt
    12061 ctttgcttcg gggtgaaacc atcggcggta atcgataagt gaacattccc caagctgctg
    12121 ggataacgat tcctttgcct ccaacctcgc ttagtgcacg caaaatctca gcaatttgac
    12181 gtacaccatt aagacgccgt tcttcgataa agtcaccgat ccacccatcg tagccgttac
    12241 aagctgtcac tactgggatc cctgtttctt tgattgcctg tttgactgtg tcgatatttt
    12301 ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc acagtctttg ataaatctaa
    12361 atttttctaa aatgtctctt gggaaaaatg cttgatcttg cgtaccgaat ttcattgttc
    12421 tcctcctcgg acttgaacgc ccattttgat actttcttct gggtgctggt ctacataacg
    12481 catgtaagct tctgcgcttt ctgcaaatgg cacaactgga tcaatgattt cttcacaatc
    12541 caagtaaccg ttcatcaata attcccaaca tgtgtcctcg attcgtttac ggttccatct
    12601 tgggtaatct gggtttggtt cactagatgc tcgagcaaag acgatttttg cattattgaa
    12661 atgtgcttcg cgaccaagat taaacccagc tggaaaaggt ttagcaaagg caacataaga
    12721 aatgatcccg ccatagccga tccctattaa agaagcttgt aatgcggcag cactgccact
    12781 cgtttcaata actgaatcta ctccacgttt atctgtcaat tcctttagac gaaaaccgac
    12841 atctgctttc ataggatcta gcgtgtaatc ggcaccgtgt ttttctgcta attctctacg
    12901 ctgcgctatg ggatcgatcc caacgatggt agtagcacct gcttttttag ctaattgaat
    12961 cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca gcatcacctg ctcgaacatg
    13021 tgcttcccgg ataccaccta aagcgaattg agcaggatcg taacatactg catttttcca
    13081 ttgatccaac cgatcaagtt tacgtagttt ataattatca acaccgtttg taatcagcgt
    13141 ttcacgaatg ggtccataag tgcagactaa ctctcctttt tgatactctg tcaccaaact
    13201 gcccgtttct tcgatcgtac cgacaatcat atttcctaat tgaaattcgc caaataccac
    13261 accgcgttgg cttccttctt ctcttggcaa gaataggcgc cattcttctg aaaattcttc
    13321 atcgataaat ggacttaatc ctctaaagtc gacaatttct gttcctggtt ttggagtagc
    13381 gaaaattact cggatacgta cttcgttctt ttcgatctca cggtcttcgt aatctttgaa
    13441 tccggctatt cttggttcta atgcaactaa ttttttcatc ttttctttcc tctttctttt
    13501 ctttttatcc ttttacgccg ccttcgacta aatcaccttt aataaatcgt tcagataacg
    13561 catacataat gatgactggt aaagcggtca ccaatgaagc ggccatcatt cgtccccaaa
    13621 tgtagtctgg tgtactgaat aatgtgttca aaccgattgg taaagtaaat ttttcggagg
    13681 tcgacaggaa aatcgaagca aataaataat cattccacga aaccataaag caataaacga
    13741 agacagaaat gattccagaa atagccaatg gtataatgat ccgaataatg atctggatcc
    13801 ggctaagacc atcgatcatt gctgcttctt caatatcagt cggaatcgtg tcaaaataac
    13861 tttttagcat gaatatcgca gtaggaagtg tttgaacgac catcgtgata atcagtgctg
    13921 tattggtatc atacaaacct agaccggaaa tgattttaaa taatggaaca acgatcaaaa
    13981 taccggaaaa catataaacg gtgtaaaagc tagcattgat tttcgagcga gctttgaagc
    14041 gtagttttga caaagcgtat gccccgctga tcccaatcac tactgcagtt acggaggcta
    14101 tcaaggagat cactaaacta tttttgaaat acatgatgaa tggaaaaatc tcagcattga
    14161 aaatatcctg ataatgttca aaagtccatt ctttcggaaa aatagtttgt gtcgttgcaa
    14221 ttgcttcttg tgaatccttg aatgatgtca tcagcatgat caaaaatgga aacaaattga
    14281 cagctaatag caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt ttatttttca
    14341 tttcaagtcc accttctttc gtgagagcaa gacgaccgct gcaataatga tgaacaatag
    14401 aatcgatata gctgctgctt ttccaagatc attgaacgca aaggctgtct gatacaaata
    14461 aacgcctaag atattgactt tattcgtcag tagatagaca tctgtaaaca tatagaacat
    14521 ccagatagat cgtaaaacaa tcacagttgc tagtactggt ttgattgccg gcagtgtcac
    14581 gatcttgaac ttctgccaag catttgcacc gtccatttca gctgcttcat aaagcgtttt
    14641 gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca tacggatagt atctccagat
    14701 agcaaacaaa acgaccataa agaagctagc aactggctgg tcgaaccaca gcggtgcttc
    14761 gttgaaaagc tgcaacctat ctactaacag atcattgacg attccataac tattattgaa
    14821 catatatttc caagcaaaaa tcaaagaaat agatggcgta acataactta ataaaatcag
    14881 tgaacgcacc gttttcctaa atctgaactt gcggttaagc aaaatagcta cggctaatcc
    14941 taaagctgta ctgcctgcta caactaagaa tgtgtaagca actgttaaac ctaaagaacg
    15001 ataaaattca ctatcactta aaatttcaag ataattgctt aatccgataa aaacggcgtc
    15061 tgagttagga ttcagcggca tatcgaaaaa actgatctga atattggaaa gcattggaat
    15121 cagcactaaa acagctaata aaagcatgct tggtgctaaa agtcccatgg ctaattttaa
    15181 gtcagaatgt ttccttggac gattcattga aacctccccc tgataatctg ccaatagcta
    15241 tctacttttt tcgaacacca ttggcagaac ttcttgttat ttcgtatttt tttgtgcgtc
    15301 agctaaagct tgatcgacat cttcgtttcc gacagttact tggttcactg cttttccgat
    15361 cacacctgag cttgtgatat ctcccatttt cgtgaaattc ttttccccga ctagtccaaa
    15421 gacttgtact tcatcaaaag cactagcaat ctcagatggc agctcgccaa aagacttgat
    15481 gacttcattt tctttgtaac catccagttc gactacttgt ttattcactg gttgagcacc
    15541 accaggagac atcaagaccc acttttccat gtttttcggt tgagataaga aagctgtgaa
    15601 tgcttcagca gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca atccagatac
    15661 tgtgccatag acagcttgat ctttttgtgt tgggatagca aaaccgatat tttctgagtt
    15721 ccccgcttca taaacagaag gcaaaatgta agttgaatag attgccattg gtgctgttcc
    15781 attcatgaaa gcatctttga tttctgtcac atcattagag cctggcatcg tgtattggga
    15841 taagttttga taaaacgaca atgcttctct catcttttcc gtatcgatcg ttacctcgcc
    15901 ttcatcatcc aaaacatttg ctccatttga caaagcaaat tgagagaacg cttgctcact
    15961 cattgttcct tcagcagtag gcatagcaat tccatattgt ttattctctt tatctgtgaa
    16021 atgttgagca atttttaaaa tatcatccca attttctggt tctgaaaaac cagcttctga
    16081 taatttttct ttgtcatacc aaataccttg aacccagccg ctaattggtg cagcaatata
    16141 gctttttcca tcttctgaac gaacaaggtt tttggctccg tcataataat tatcttctcc
    16201 agcttcctcg ataacgtttt ggactgccgt ctgatcaatc agttcatctt tatccatcac
    16261 tttagcaaaa tcctgactga cttccattac ttctggtaat ttaccggaac gagctaacgt
    16321 aacgattttt gtattgaacg ctgattcttc taccggcact tgcttgattt tgatgtctgg
    16381 attttctttt tcaaaatcag caaccagttc attgatcaca cttaatcgtt cctgttcgac
    16441 agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt gactttcacc
    16501 acaagcagta agaagaaatc cagtagctag cagacaggta gtaacaataa tttttttcat
    16561 ttttttccac ctcttatttt attattttca gccacatata ttcatacggc tggaatatca
    16621 actttcgttc ttgactgcaa tggataattt gtccagaaaa caggtctagg taatctcctt
    16681 ttggtaaaag ataacctgct gattgattgg aaaaattatg gataactaag actttctctt
    16741 ccttcgcttc gcgaataaaa ctgataaaag aatctgctga gtccagtaag taaatcaaac
    16801 tattcggatg gaaggctttt tctttttttc gaatagatag catctgggtc aattcaccat
    16861 atactgctgt tcttaatgga tctgtctgca actctttctc aatatctgct aacaaaaatt
    16921 tttgccgatt gatttttcga ttctctcctg ttttttctac gcctttgtaa tcatttcgac
    16981 ttcccagcac actttgaata taaattgccg gcacgcctgg taacgttacc aaaaatgaat
    17041 gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa cttaacgcat
    17101 ctagatacgt agcgtttatc tcatatggac tggtcgttcc atctgggttt ttcttgtagt
    17161 ttactcgggc accctctttc ttcagctgtt ccactaattc aaggatcgtt ttttccggca
    17221 gaatacctcg taccggattc aatccgatac cgtcatggga ggctaaaaaa ttaaaaaaag
    17281 ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc tttcaactta cttgtattac
    17341 ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc atctgtgctt
    17401 cattctcgcc attcccaaaa taactgatat tatctttgtg tggcacattt gtctcagtga
    17461 tcaataccgt tcctggagct gcttcatcag cgattgcacg gaataattgg atgattttat
    17521 gcgtttctgg caggtgtata caagaagttc ctagttcttt ccataaaaat cccacagcgt
    17581 ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa aagaatatcg atcattttca
    17641 ataaaacttc tggattttta aaattcaagt ctacttgatc ttcgctaaaa gtcgtccata
    17701 cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag taacgggctg gtccttggtc
    17761 tagtcacgct gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct ttgtacgtat
    17821 cattcaagtt taaatattcc tgaaaccaag tactttcggc agacatatga ttgcagacaa
    17881 agtcgaacat taaccgtgtt tcgcttctca tttcttctat atcttcccat gtgcccaaac
    17941 ggtcgttgat tgcctgataa tttacaacgg agaaaccatc atcagatgta tatgggaaaa
    18001 atggcaagaa atgtacaact ctaaatgtat ctgccaaata cttttgataa aatttcttaa
    18061 atgtacgcaa agttggttgc tgctcttcaa aaaattgatc cccataagta atcaagaaaa
    18121 tatcttcttc gtcccaatgc agtttgcctg cctggccttt cttctgctga taaacactga
    18181 ctttttgttc aatcatagag gcggttttct cagacaaatc tgagtcgcga taaatcgctt
    18241 gtaaaagttt tgtatacatc ggattcccct ttcttttttg ggagcgttcc caacgatttg
    18301 atcatattat ggaaaacgct tactgtcaat agcatttttt taaataattt tgatatacac
    18361 aaaaatacaa ataagaaagc ttatgtatca ataattaata gcttcttaaa aaaatatggt
    18421 ctctcatgat catgcaagct attcctaaat tttcaaaatc atagacaaaa aagaatgtct
    18481 attgtactat ggaattattc taaaacgctc ccaaaaagga ggtgcatata aaattgaaac
    18541 ctactattta tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc tccagagtat
    18601 taaataacca gccaaatatc tctgataaag caaaagcaag tgtacttcaa gcaatcaaag
    18661 aattgaatta tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt tttgacgcga
    18721 ttctagcatt atctcatcgt acttctcata cgacctatgg caatcctttt ttttcagatg
    18781 ttattcaatc catttctgcc gtttctgaac aaaataactt cgacttgatt ctacaaacgg
    18841 caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa aaaatgatcc
    18901 gaggaattat tattttaagc tctacgatgg atgaaacact tctttttgaa ttggatcaat
    18961 ataatattcc tattgtggta atcggaaaag tcggaaacga ctacacgaac atctactcag
    19021 tggacacgga taatttccag gatagttacg atttggtgga aacactgatc aagaatggac
    19081 accgtcatat cggctgtctc catgctccga cagaagttta tgtatccaat gatcgtctca
    19141 atggttaccg tcaatgctta tttgatcacc acctagatat ccgtaatgaa tggattatag
    19201 acggcggctt tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg aagctacgag
    19261 aaagaccaac tgcgattttt tctaccgatg cattaaaatc attgagtatt tacaaagtgg
    19321 cagaacaatc tggaaagcag atacctgatg atctttcgat cgcaggattc agtgatccct
    19381 tattgtcctc cctattttcg ccacagttga ctcggataga cattccgaca aatcagttag
    19441 gaatcaaagc cacagaactt ttattccaac taatccacca ccaataccct gaagaaaaaa
    19501 gaatcattct ttcaaccgag attactctca cagattcgat ccaacaacaa gaaatgtaga
    19561 acagaattat aaaagaacag aaacactgag gataagtcag ttactctgtt cttttttcaa
    19621 tcactcatcc ctcgttaccc cctcccggta ttttagcgaa tttttcttat tttccatctg
    19681 ttttagatag ctgtggataa ctataatgtt tgtgttaaac tattttttcc acagggaaga
    19741 aaaaactatt tttaaccacg ttgtgtataa gtttttatag ttcgtttttt taaacacatt
    19801 tacccttatt tcaatccatg ttatttgggg atacttatct ctaaaacata aatagaataa
    19861 cagacatgtc tactggtttt gttttattcg aggaattatt caataatagc tagcaaaata
    19921 ttaaaaataa ccgctctatc ttcccatcaa tttattttgc attcaaaaaa tgatctatct
    19981 ttacttttgt tgattgactt ctcttggaag atgatcgttc cgatacctta attggatacg
    20041 aaccgatgtt gttaagatcc tttcccactt ttcttctagt tagacaaatt attttagaca
    20101 aaccaaaaaa gaggttgtga caataatttg tcacaacctc ttttttaaaa tatctatttt
    20161 gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt caatatctga aatactgtag
    20221 acattttcgc ttgctacacc gacttctttt ggttccatgt cacgataacg ttgcatacca
    20281 gtaccagctg ggatgatctt acctataata acattttctt tcaaaccaag aagaggatct
    20341 ttcttaccgc ggatcgcagc atccgtcaag acacgagttg tttcttggaa tgatgcagca
    20401 gataagaaac tatttgtttc taatgatgct tttgtgatac ctagtaagac aggtcgagaa
    20461 gttgcaggaa cgcctcctga gactagcgta ttgtagtttt gatctttaaa gtctgcgatg
    20521 tctaataatg tacctggtaa gatatctgta tcacctggat ccattacacg aactttacgc
    20581 agcatttgac gaaccattac ttcgatatgc ttgtcgccga tttctacccc ttgcatacgg
    20641 taaacacgtt gtacttcacg aagcaagtag ttttcaacag ataatacatc gcgaacttgc
    20701 aacaattgtt ttggatcgat tgatccttct gtcaatggtg caccacggtg gatcatatcg
    20761 ccttctgcta ctttcatacg tgctgtataa ggaacagtat acgtacgtgt atctgttttt
    20821 cctttgattg ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc gatgacatcc
    20881 cctgttactt ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa gatttcttgg
    20941 acacgaggaa gaccttgagt gatatcgtct ccggcaaccc cacctgtatg gaatgtacgc
    21001 atcgtcaact gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc aactgcttct
    21061 ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt gcagacacca
    21121 tgttttgtat tacatgtgaa gacagaacgg attgtcactg tttcgacacc tgcatcaacg
    21181 atttgttttg cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa ttcatttgtt
    21241 tcaggatgac gtacttcttt acgggtgtaa cgtcccacta aacgatcttc taatgattcg
    21301 atgatttcat ttccctcgcg gatggcttgg atttcaagac ctcggtcagt accgcaatct
    21361 tcttcacgga tgattacatc ttgggccacg tcaaccagac gacgagtcaa gtaacctgaa
    21421 tcggcagtct tcaaggctgt atcggtcatc cctttacgag ccccgtgagt tgagataaac
    21481 atttccaaga cggatagtcc ttcacggaag ttcgaaatga ttggcaattc catgattcga
    21541 ccattaggag cggccatcaa accacgcatt ccggcaagct gtgtaaagtt ggaaatgtta
    21601 ccacgagctc cagaatcaga catcatgaag attggatttt tcgcttccaa accttccatc
    21661 agtttttgtt ggatgctgtc ttttgcagca ttccaaacag caatgacacg ttcataacgt
    21721 tcgtcatctg tgatcagacc acgacggaat tgttttgtaa tagtttcaac ttgtttatgg
    21781 gcattttcaa tgatttcttg tttttcattc agtacactga tgtcagcgat ccctactgtg
    21841 ataccggcat gtgtcgaatg tttgtaccct aagtctttca tacggtcaag catcttagaa
    21901 gtttctgtta ctttgaatcg tttgaagact tctgcgatga tgtttcctaa gtttttcttc
    21961 ttgaacggta atactaattc ttgctctttg atatgtgcag gaatatcagt accagcttca
    22021 acaaagtatt tgtccggtgt ttggacggtc aagttgaagt ctgttggttc attcagataa
    22081 gggaattcag gaggcatgat ctcgttgaag atgatcttac caactgttgt gatcatcgta
    22141 cgttcttttt gccattcagt aaatggtttt tcacctaatg agttagggtt cacaccgata
    22201 cgtgaatgta aatgaacata tccgttgcgc catgcgataa cggcttcgtt ttgatctctg
    22261 aagaccattc cttcgccttc gcgtccttct tcttccattg tcaagtagta gttacctaga
    22321 accatatctt gagaaggagt aacaactggt ttaccatctt tagggttcaa gatgttttga
    22381 gcagctaaca tcaacatacg tgcttctgct tgtgcttctt cattcaatgg aacgtggaca
    22441 gccatttggt ctccatcgaa atcggcatta taagcttcac atacaagtgg atgcaaacgg
    22501 attgcgcgtc cttgtactaa aactggttcg aatgcttgga taccaagtcg gtgaagcgta
    22561 ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt cttctaaaac atcccatact
    22621 tcgtcttctt gacgttcgat tttacgtttc gcatttttga tgtttgaagc tagttcgcgt
    22681 tgaacaagct cacgcatcac gaatggtttg aataattcaa tggccatttc ttttggaagt
    22741 ccacattggt acatcttcaa gaaaggtcca acaacgataa cggaacgacc tgagtagtcg
    22801 acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa catatgagaa
    22861 agagatttca atgggcggtt acctggtcca gtaactggac gtccgcgacg accgttatcg
    22921 atcaatgcat ctactgcttc ttgcagcata cgtttttcat tttgaacgat gattccaggt
    22981 gcattcaagt ctaacaaacg tttcaaacgg ttattacggt tgattacacg acggtaaaga
    23041 tcattcaagt cacttgttgc aaaacgtcca ccttctaatt gaaccattgg gcgtaaatct
    23101 ggtggaataa cagggataac gtccataacc atccagcttg gttgatttcc tgaagcgcgg
    23161 aatgcttcta aaatatccaa acggcggatt gcacgtgtac gtttttgtcc ttgtgcagat
    23221 ttcaattctt ctttcaattc ggctgcttct gcatctagat caacattatc aagtaattgt
    23281 ttaatcgctt ctgcacccat agcagcttgg aattcttgtc catattgttc acgtttttcg
    23341 cgatattcgc gttctgtcaa caattgtttt ttctctaaat tcgtatttcc tggttcgatg
    23401 actacatatg aagcaaaata aatgatttct tccaaagcgc gtgggctcat gtctaataca
    23461 agacccatac gtgaaggaat ccctttaaag taccagatat gtgaaacagg agctgctaat
    23521 tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag taacttccac tccgcatcga
    23581 tcacaaacga ttcctttata acggatacgt ttgtattttc cgcaggcaca ttcccagtct
    23641 tttgtaggac caaaaatccg ctcacagaac aacccttcac gttcaggttt taaggtacga
    23701 tagttaatcg tctcagtttt ttttacttca ccgtaagacc agcttctgat tttttcggga
    23761 gaagccaaac ctatttgcat actttcgaat ttatttacat cgatcaaaag gggttccctc
    23821 catttcattt aatggcaaac cgcaagacgg ctgatcttcg accgccttgc agctcataga
    23881 ccggattagt ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc gtctttttca
    23941 tcttccactt gttcagcagc tttcttttct aattctttgg cagtttgctg ttcggcaaat
    24001 tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc gcgaagttcg
    24061 atctctgttt cttcgatatc caagacacgc atgtccagac ctagtgattg caattctttc
    24121 accaatacgc ggaatgattc cggtacacct ggttttggaa ttggttcacc cttgacgatt
    24181 gcttcgtatg ttttcacacg tccgactacg tcatctgatt tgtatgtcaa gatttcttgt
    24241 aaagtataag ccgcaccgta agcttccagt gcccatactt ccatttcacc aaaacgttgt
    24301 ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa ccaatgagta aggtccgatt
    24361 gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta catcacaccg
    24421 acagacacac gtccatcaaa tggttcacct gtacgtccgt cgtacagaac agttttcgca
    24481 tcgctggcca taccggcttc acgaactgtt tcccatacgt cttcatcact tgcaccatca
    24541 aatactggcg ttgctacgtg gatacctaac tgacgtgcag ccatacccaa gtgtaattcc
    24601 aatacttgcc cgatgttcat acgtgaaggc acccctaaag gattcagcat gatatcgatt
    24661 ggtgttccat caggtaggaa tggcatatct tcttccggca taatacggga tacaacccct
    24721 ttatttccgt gacgaccggc cattttatcc ccttcatgga ttttacgttt ttgaacgata
    24781 taaacacgaa ctaacatgtt tacgcctggt gacaattcat ctccagcttc acgagtaaag
    24841 attttcacat cgtgaacgat accgccgccg ccatgtggta cacgtaatga tgtatcgcgg
    24901 acttcacgag ctttttcgcc aaagatcgca tgcaatagac gttcttctgc agataattct
    24961 gttacccctt ttggcgtgac tttccctaca agcaaatcgc catcttggac ttctgcacca
    25021 atacggatga tacccatttc gtctaaatct tttaatgcgt cttccccgac attagggatt
    25081 tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg cttctgattc atattcttca
    25141 atatggatag aagtgtatac gtcatctttt accaaacgac ggctcatgat gatcgcatct
    25201 tcatagttgt acccttccca agtcatgaaa ccaactaaaa cgttttggcc tagagccatt
    25261 tcgccttgtt ccatagatgg tccgtctgcc agagtatccc ctttttcgac tttctcaccc
    25321 aaatgaacga ttggacgttg gttgtagctt gttccggagt ttgaacgacg gaattttgtt
    25381 acatcgtatt tatctaatgc accgttgtcg cggcgtacgc ggatttcaga agcatcaacg
    25441 tattcaacga caccgtcatg tttacataac aaagcagctc ctgagtcatg cgctgatttg
    25501 tattccatac cagtaccaac ccatggtgct tgaggattga tcaacggcac tgcttgacgc
    25561 tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt tttccaagaa aggaatacat
    25621 gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca tgtagtctac tttatcaata
    25681 gatacttcta agttttcact ttgagcacga gccataacaa cgtcttgagc aaatgttcca
    25741 tcttcattca atgggctgtt tgcttgggct acgatgtaat ggtcttcgat atcagctgtc
    25801 aagtagtcga tttggtcagt aacacgacct gtttcacggt ctacacgacg ataaggtgtt
    25861 tcgataaatc cgaatttatt tactttcgca taactggaca aactattgat caacccgatg
    25921 tttgggcctt caggagtttc gatcggacac atacggccat agtgggagta gtgaacgtca
    25981 cgaacttcat aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc tgaaagacga
    26041 cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca tgaactgtga caactgagaa
    26101 gaaccaaaga attctttgat acttgccaca actggacgga tattgatcaa ttgttgaggt
    26161 gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa ccacacgttc catacgtgct
    26221 agcccgatac ggaattggtt ttgtaaaagt tcaccgactg aacggatacg acgatttcct
    26281 aagtggtcga tatcatcgac attgccgata ccttccatca agttcaagaa atagctcatt
    26341 gaagcaatga tgtcggctgg acgaaccgtt tttacagctg cttctggata gccgtttccg
    26401 atcacgttta cttcacgttc agggtctctt ggtgaaaata ctttgatgac ttggacagtc
    26461 attggatctg tgaccacacc gtcttctgat ggatagtaag tcactgaatt taggccatta
    26521 tcaatgaaag gagctagtgt ttccatgact tggtgagtca gaactgttcc tttttcaacg
    26581 atgatctcgc ctgtttctgg atcaactaat gtttcagcta atgtcagatt caatagacgt
    26641 gttttcaaat ctaatttttt gtttactttg taacgaccaa cgtttgctaa gtcgtaacgt
    26701 tttggatcga agaaacgagc attcagcaag ttacgtgagc tgtctgctgt ttttggttcg
    26761 cctggacgca aacgttcgta aacgtctttc aaaccttctt ctgttcttga gtcgcttgcg
    26821 tttttatgca agtctttttc gactgtattg cgtaatgttt cgctgtcgcc gaagatttca
    26881 aagatcgtat catcggaacc aaatcctaac gcacgaacta atacggttaa aggaatctta
    26941 cgtgtacggt cgatacgtac ataagagatg tcttttgcat cagtttccat ttctagccaa
    27001 gctccacggt tagggataac agtagaaccg aatccttctt ttccgttttt gtcgactttt
    27061 ccatggaaat agacacctgg tgaacggact aactgagaaa cgattactcg ctctgccccg
    27121 ttgatgatga atgttccttg ttctgtcatt aatgggaaat caccgaagaa tacttcttgt
    27181 gctttgattt cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa tggtgcagag
    27241 tagtttgcat catgcgcacg agcttctgct actgtatact ttggttcttt caattcataa
    27301 tctacaaatt ccaaagatag attgccattg aagtcatcga ttggcaaaat gtcttcaaac
    27361 atttctctaa gtccctcatc taagaaccat tggtaagagt ctgtttgaat ttcgatcaaa
    27421 tttggtaatt ccaacacttc actgattctt gcgaaacttc tacgttcgcg atgttttccg
    27481 tattttacta cgtgtccagc caagctcttc acccctctaa aatgtcattc aaaaagacta
    27541 cccgatcaat tgttacaata atatttcaaa tattattttt aggtaacaac agacattttt
    27601 tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc ttacttcgtg
    27661 agcttctact tttgtttcct tatattaaag ccgaaacgga caatatttcg tttctgcctt
    27721 ttcttcatca gatagttttt gcaacttcat atactacact tattttctat aaaagtcaac
    27781 acttcctggc gtttcttctg ctttttcgca acattttctt aatgttttca attttatgtt
    27841 ttcaattatc tgacaattct taattgacag acctttttat ctcggttata ctttagaaaa
    27901 ttcattttgt ggaggaactt ctatgcgaac caacatcaac caaatcgcac caacacaact
    27961 gaataacttt tactttagct actttagata ggtttgtatt catcatgatg gatacaaacg
    28021 ttaaacgaca gtttgtatct atgatggcta aagaactttg tattgcagca aaaagccacg
    28081 tagacaaaat gctaagtggc tgagcagtga aaatacaaag taaaagtatc aagcattcta
    28141 ctttttcaat ttattttccg acttttcaat ccatttggac attttgtctg aatggatttt
    28201 ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa atataatcat cggattaggc
    28261 gctgccctgc tactagcttt aggaggttgc gcacaaaaag acaattccgc agaggcatca
    28321 gatggacaaa aaacaattgg cgtattgcaa cttgtacaac ataactcgtt agatgctgcc
    28381 tataaaggat tcaaagaagg attagcagaa gaagggtata aagaaggcga taatctaaca
    28441 atcgattatc aaaatgccca aaacaatcaa gacaacctga aaagtatgag tgagaaactc
    28501 gtcaaagaag atcctgattt gctacttgga atcgccactc cagcagcaca atctttatta
    28561 aatgaaacaa ccactatccc gatgactgtc acagcggtca cagacttgga acaagcccat
    28621 ttagtcacat ccaatgaaaa acctggcggc aatgtgactg gcacgacaga tatggtgcca
    28681 atcgaaaaac aaatcggctt actgttaaat atcgtgccag acgccaaaac aatcggcatc
    28741 atgtataaca acggtgaagc aaactctaag atccaagctg atttggcaga aaaagaattg
    28801 aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt ccacaaatga tgtccaacaa
    28861 gtgacgaaaa gtctagctaa agatgtagat ggaatctata ttcctactga taacacgttc
    28921 gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc tgtcgttgct
    28981 ggcgcagttg atcaagtcaa agacggcggt ctagcaacag taggaatcga ctacgaaagc
    29041 ttgggcaaac aaacagggat catggcagca aaaatcttag acggcaagaa tccaagtgaa
    29101 cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca ataaagacat ggccaaagca
    29161 ttaggcatca atccagacag catcaaagaa gctgaataaa acacaggagg attctcatga
    29221 caaatttgct ctctcttttc ttatcatctg catcgcaagg ggtcttatgg gccctacttg
    29281 cgattggtgt atacgtcact ttccgtatct tggacattgc cgacttgact gctgaaggaa
    29341 gctttccttt aggtgcaggg atcgcggcag taagtattac aaatggctat catccattga
    29401 cagcttgtct tctaggcttt ttagggggtg cggcagcagg gcttgtttcc ggactgcttc
    29461 acacaaaatt gaaaattccc gcccttcttg cagggatcat caccatgact ggtttatatt
    29521 ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt attgggtcag ccaacgatct
    29581 ttcattgggt cgagtcattc ggcataacaa aagacaacgc tgtactgatc gtcggtctgc
    29641 tttttgcttt gatcgttgta tgtttacttg ttttgttctt acgtacagaa acaggacttg
    29701 ctattcgtgc aactggcgat aatctggcaa tgagcgaagc aaacgggatc aacacagata
    29761 atatgaagat catcggttat atgatttcta atggatgcat tgctctttct ggctccttac
    29821 ttgcgcaaaa caacggcttt gctgatttaa attctggtat tggaacgatc gtaattggac
    29881 ttgcttctat tatcatcgca gaagttttgt tacgtaacca gccactattt ctccgtctct
    29941 tgactgtcgt tttaggtgcg gtcatttatc gttttatttt agcgttagtc ttcgaactga
    30001 atgttgaacc aagtgattcg aaacttgctt ctgctttcgt gctagtgatc tgtctttctt
    30061 tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt agtgtcaaat
    30121 gaatgacgtc ttaacattgg aaaatctcca tcataccttt gaaaaaggaa cgatcaacga
    30181 aaatcatgtc ctgaaaggca ttaatctttc cttagatccc ggcgacttcg tgacgatcat
    30241 cggtggaaat ggggctggta aatctacttt gctgaacagc atcgccggta cattttctgt
    30301 cgatcagggt caaattttat tgaatggaaa aaatatcacg aaaaaatctg ttgtcgaacg
    30361 ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta gggacagccg ttcgtttaac
    30421 ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct tcttccgtgg
    30481 tgtcaaacca caagaccgaa catttttcaa agaccagctg gttcggctaa atttggggtt
    30541 ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga ggacaacgtc aagctattac
    30601 gttattgatg gccacattac agcgtccaga actgatctta ttagatgaac acactgcagc
    30661 gcttgaccct gctacttcca aaacggtgat ggaattgaca gaaaagctga tcactgagca
    30721 gaatctcact gcttttatgg tcactcatga catggaagac gcgatccgct atgggagtcg
    30781 actgatcatg ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg aaaaacagca
    30841 gctcacagtg ccccaattat tagcgttgtt caaacaaaac agcggcacgg cattaaaaga
    30901 cgatcaagta cttttatctt cataatcaga aagaggatgg gacaaaaaat gaacgcccac
    30961 cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc gttgtttctt
    31021 cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg agcttgatgc
    31081 catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt ttctttccgg
    31141 agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag ccatcaaaga
    31201 attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt acctgccctc
    31261 tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc ctgatttgaa
    31321 agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg cagcagctca
    31381 aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc gtggacgttt
    31441 tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct tgctccatcc
    31501 agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg cctgtgtgaa
    31561 agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg atatctacat
    31621 ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg aaagcggcag
    31681 gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa atcattttcc
    31741 agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta gtgtcacaag
    31801 gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc acccagaaaa
    31861 agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt cgttcaatca
    31921 aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg aactgatcgt
    31981 ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca catctatccg
    32041 tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg tccttttttc
    32101 tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct atcctctaga
    32161 aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg aatcaatgca
    32221 aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc ttcctttttc
    32281 aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg acttattttg
    32341 ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg tctcgcatag
    32401 atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg cattcctcgt
    32461 gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg ttccagcctt
    32521 cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag atcttctatt
    32581 tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg ttcgttatct
    32641 gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg agaagttcct
    32701 gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag ccaagtcatt
    32761 gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc aatagagacg
    32821 ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca tagcatatct
    32881 aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc taattctagg
    32941 atacctactt atatgttact acacaccctt attttttaca ctttaacagt caccatcatc
    33001 aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac aagaagcaac
    33061 aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac gaacagttta
    33121 agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag gggaaaaatg
    33181 aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt ttcgtatggt
    33241 gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta taatgatcta
    33301 actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc taaaaataag
    33361 acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga tgcaaaaaat
    33421 aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta tatacaaaaa
    33481 actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat gaataaattt
    33541 gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt caatggatcc
    33601 aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca tgaatatatt
    33661 gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc aacgatcact
    33721 ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac aatcgattta
    33781 actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt gttaacgatg
    33841 aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa tgtcatcaac
    33901 agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc aaatcatgaa
    33961 acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat gactgatttg
    34021 acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat ccgcattact
    34081 caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc taccaatcgt
    34141 tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga cacaagcgac
    34201 agtacagata catctaccac aagtgatagc tcagattcat caacttcaag cgacagtaca
    34261 gatactacct ctaccacaag tgatagcacg gatac
//
LOCUS       DARDHD010000010        32669 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000010 DARDHD010000000
VERSION     DARDHD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.010"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..598
                     /locus_tag="SDA06_000420"
     CDS             278..598
                     /locus_tag="SDA06_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG4227717.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            752..1657
                     /locus_tag="SDA06_000421"
     CDS             752..1657
                     /locus_tag="SDA06_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227718.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1654..2427
                     /locus_tag="SDA06_000422"
     CDS             1654..2427
                     /locus_tag="SDA06_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4227719.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2608..4032)
                     /locus_tag="SDA06_000423"
     CDS             complement(2608..4032)
                     /locus_tag="SDA06_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227720.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4243..4689
                     /gene="nrdI"
                     /locus_tag="SDA06_000424"
     CDS             4243..4689
                     /gene="nrdI"
                     /locus_tag="SDA06_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG4227721.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4754..6736)
                     /locus_tag="SDA06_000425"
     CDS             complement(4754..6736)
                     /locus_tag="SDA06_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4227722.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6750..7646)
                     /gene="murQ"
                     /locus_tag="SDA06_000426"
     CDS             complement(6750..7646)
                     /gene="murQ"
                     /locus_tag="SDA06_000426"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG4227723.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7649..8710)
                     /locus_tag="SDA06_000427"
     CDS             complement(7649..8710)
                     /locus_tag="SDA06_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4227724.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8921..9487)
                     /locus_tag="SDA06_000428"
     CDS             complement(8921..9487)
                     /locus_tag="SDA06_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4227725.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9515..11017)
                     /gene="eat(A)"
                     /locus_tag="SDA06_000429"
     CDS             complement(9515..11017)
                     /gene="eat(A)"
                     /locus_tag="SDA06_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4227726.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11275..12396)
                     /locus_tag="SDA06_000430"
     CDS             complement(11275..12396)
                     /locus_tag="SDA06_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4227727.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12522..13463
                     /locus_tag="SDA06_000431"
     CDS             12522..13463
                     /locus_tag="SDA06_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4227728.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13613..14509)
                     /locus_tag="SDA06_000432"
     CDS             complement(13613..14509)
                     /locus_tag="SDA06_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4227729.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14778..16118
                     /locus_tag="SDA06_000433"
     CDS             14778..16118
                     /locus_tag="SDA06_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227730.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16336..16779
                     /locus_tag="SDA06_000434"
     CDS             16336..16779
                     /locus_tag="SDA06_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG4227731.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17283..18701)
                     /gene="nhaC"
                     /locus_tag="SDA06_000435"
     CDS             complement(17283..18701)
                     /gene="nhaC"
                     /locus_tag="SDA06_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4227732.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19104..20327)
                     /locus_tag="SDA06_000436"
     CDS             complement(19104..20327)
                     /locus_tag="SDA06_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG4227733.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20358..21197)
                     /locus_tag="SDA06_000437"
     CDS             complement(20358..21197)
                     /locus_tag="SDA06_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4227734.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21214..21894)
                     /locus_tag="SDA06_000438"
     CDS             complement(21214..21894)
                     /locus_tag="SDA06_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227735.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21896..22969)
                     /locus_tag="SDA06_000439"
     CDS             complement(21896..22969)
                     /locus_tag="SDA06_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227736.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23025..23263)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23306..23539)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23616..24287)
                     /locus_tag="SDA06_000440"
     CDS             complement(23616..24287)
                     /locus_tag="SDA06_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4227737.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24374..27052)
                     /gene="mgtA"
                     /locus_tag="SDA06_000441"
     CDS             complement(24374..27052)
                     /gene="mgtA"
                     /locus_tag="SDA06_000441"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG4227738.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27711..28148
                     /gene="ndk"
                     /locus_tag="SDA06_000442"
     CDS             27711..28148
                     /gene="ndk"
                     /locus_tag="SDA06_000442"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG4227739.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28170..29036)
                     /locus_tag="SDA06_000443"
     CDS             complement(28170..29036)
                     /locus_tag="SDA06_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4227740.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29196..31289)
                     /locus_tag="SDA06_000444"
     CDS             complement(29196..31289)
                     /locus_tag="SDA06_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4227741.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32038..32661)
                     /locus_tag="SDA06_000445"
     CDS             complement(32038..32661)
                     /locus_tag="SDA06_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227742.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         9442 a         6823 c         5783 g        10621 t
ORIGIN      
        1 ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat tgcaaagcat
       61 tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta ctaaaaagta
      121 tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata caatctgcca
      181 ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga ttttcctctt
      241 tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg aattaatcat
      301 agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg gtttttctaa
      361 aatcaattat tctttttaca cagtgattgg catgattgct agtttttact ttttgtcaaa
      421 ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga ttggcgcagt
      481 tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt ccacttggtt
      541 ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg gccactagac
      601 acttggaata agtactgatc agaaaaaata agccttttta tttaatattc aattttttta
      661 tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca ggtgtatgct
      721 tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc aagaattatc
      781 caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat cccctggcaa
      841 aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga aagctatttt
      901 aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga tttctccctc
      961 tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta tctatccatt
     1021 cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat ctgctatcga
     1081 cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga aatcctactc
     1141 gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc cgtctatcgt
     1201 tattttagat gagccgatga acggtttgga tccacaagca acaagagatg tgcgaaaaat
     1261 cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact tactcagcga
     1321 gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca tcaaacaatg
     1381 tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta caacaaatga
     1441 tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc ctgataaaat
     1501 caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat tgtctgcaca
     1561 aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt ctctattgga
     1621 aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa acaagaatta
     1681 tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt tgttttgctc
     1741 ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt tgaaatgact
     1801 gctcagcttt ttagtgccac atcttggatt gtctttatca tgattgcagc tgcaagcacg
     1861 atcatttcga tggaagcaca atatggtaca ttgaaaaatc tcctttatcg aaagtattct
     1921 cgaggagaaa tattagtaag caaatggatc acattagtta tttattctgt ttatttatac
     1981 cttttagcta ttattgtcac tgtgttgatg aaactgattc ttttcccttc gatttccttc
     2041 acggagaagg tttctactgg tcaaacactg atccaaagct tgttcactta tactttgggt
     2101 agttatatcg gtttatggct gattttaagt ttagtgttga tgattgcttg ctttatcaat
     2161 agttccggcg catccatctc agcaggaatc gtcttttatt ttgcttcgtc aatcatatct
     2221 ggtatcctta ttgcactcat ccaaaaatgg gagtggatca aatggaatcc gatcagtatg
     2281 ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga agcaattgac tcatctttct
     2341 actaatcaaa tgctattcgg taatttagct tacatcgttt tgttcttagc attaggatat
     2401 ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt gaaagagacg
     2461 ttcagcctta ttgccgattg gctgtctctt aaacaccgtt tattctgaaa aaggagctgt
     2521 gacaacattt gtgtcacagc tcctttccta gtatgttttt gaataaacga caggctcaca
     2581 acctctcttt atacttcttt tatacttcta tacgtgaagc atcccagtgg caaataaaac
     2641 cattgcaatc actccgacca tcgtcacacc tataagtaaa atcttatcta agtggatatg
     2701 ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc ctggcaagaa
     2761 aagaacaaga acaagcatta cttcattaaa ggctacgact agcatagccc atacttgaaa
     2821 ggcacttgct aaaagcccta ctaaaaattg gacccattct ttccgctgat aagaaagttt
     2881 catttggtac aatcctaaga aaagccacgt gaagaaaata gctgctgtac caagtgtgta
     2941 agcaaagtta taagcactgt cagtgaataa taatgaaagt aaaaagacat tcgtacataa
     3001 cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc cccatgcttt
     3061 aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca atcggtaaga tcgtccatga
     3121 gagccaaaca ccaactaaag aaatgatcaa accgccgttg atcacgactg ctccccattt
     3181 accaaccatc atttcaaaca catagcccat agcaggctgc gccttgatcg tttgcagctg
     3241 attcatcgtc attgtcccat atggaagcat ggaaaccaag acgtaaataa taatcaagcc
     3301 gaatatccct agaatcgttg ctttcccaac atctgattgt ttttttgctc gtgaacctac
     3361 gacagaggct ccttcaatac caacaaatac ccaaagcatc accatgatgc aactcaatac
     3421 ttgactggaa aacgggatcg gctgtccatt cgcattcaat ataatgtcgc tagaaaagtt
     3481 atcaatgaat tgttggatcg aaaacgtgct gatagctact acaataaata aaaataatgg
     3541 cactaattta cagaatgtga cgatagcatt gacgaaacta gcattttcga ctccccgatt
     3601 cactagccat gcatatcccc ataaaaagac tgtactcatc acgatcgatg ccagattttg
     3661 cccaccttca aatactggga agaaatatcc taatgcgctc atcaacaatg ttgcaaaagc
     3721 tacgttgcct aaccagcatg tgagccagta accccaaccg ctgataaaac cagcgaaacg
     3781 gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac gttttttatt
     3841 gatattattg atcgacaagg agagcatcaa cattgctgtt cctacgagta cccaactgaa
     3901 aatagctggg ccgccagccg atccggagat gtccgtcata ataccaaata cgccacctcc
     3961 aatcgctgaa ccaattacta aggcagcaag tggaattaaa ccaattcctt ttttctgttg
     4021 ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa gtataacaaa
     4081 ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc taccggcttt
     4141 tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaactta
     4201 aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat tttatatata
     4261 tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc tgaaaaaatg
     4321 catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat
     4381 tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga cggcggaaat
     4441 ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata tctggaacat
     4501 gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa cttcaataat
     4561 cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt cttggcagat
     4621 tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt agcagaaaac
     4681 aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt tcgtttctat
     4741 tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt gctttacctg
     4801 ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg atcatggatg
     4861 tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc gtatcaggtg
     4921 tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag tcaactgtgt
     4981 tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca atagcgtgtt
     5041 tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt ccgttttctg
     5101 gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca cttgctttat
     5161 ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt tcagctgaag
     5221 tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc gctaattctt
     5281 ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag ccaccagcat
     5341 aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc aatgggatca
     5401 aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt ccgatcactg
     5461 ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga ggtaatgtta
     5521 ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct cctttgatca
     5581 tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct gcacccactt
     5641 gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt tcatcaatca
     5701 tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc ggcaagaata
     5761 atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag ttgatcccac
     5821 cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt tcaatcagac
     5881 cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca ggtactactt
     5941 tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca cctaggatcc
     6001 caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt gcttccggat
     6061 tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt gttgcaccaa
     6121 aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc ccgtttttca
     6181 agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt ccggctgtga
     6241 ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca aatgcaggaa
     6301 tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta gatggttttt
     6361 gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg tcttttccag
     6421 atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct aatttcacgc
     6481 ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg atgatgactt
     6541 gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat tcagagacat
     6601 ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg acaatgcttt
     6661 ctacattgcc catgccgcct gcccctttat aaatttgttt tgccagttct tccaaagtca
     6721 gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa tccgtttgct
     6781 tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat gacgatagcc
     6841 agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta atcagcttct
     6901 gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt tggtttgaca
     6961 tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt agaaatcata
     7021 ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt caaaaattct
     7081 ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc ttgtttattg
     7141 caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc accgatcaca
     7201 tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt caaattcagc
     7261 ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac agctacagtc
     7321 attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac accgaatgtc
     7381 ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc tcccatataa
     7441 atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg ttcgatactt
     7501 gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat tagttgaacg
     7561 gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg ttccgttgtt
     7621 aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata aattgatagt
     7681 tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact ttagctacta
     7741 cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca cccatatagc
     7801 gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct gtatttcttg
     7861 ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca tctcttgcat
     7921 cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca tctacaggtt
     7981 ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc tgaacgtttt
     8041 ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat agatctagag
     8101 cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct tgatatagtg
     8161 ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct tgaattttga
     8221 gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga cgtggataat
     8281 aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat tcatcgatat
     8341 ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac cagtcggcaa
     8401 tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt gctttcaaag
     8461 gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa atatcgacca
     8521 tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc ttttgatatg
     8581 ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag ccattggatg
     8641 ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata aatattgaaa
     8701 agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac tagagtatat
     8761 atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat ttctaaaggt
     8821 tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta agcaaaagca
     8881 gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa gaaaccaatt
     8941 ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga aaataagcat
     9001 tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct tcgtcttcga
     9061 tgcattcttt caataaagga acgatccgtg catacacatc taagactgac tccccatttg
     9121 ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg cttggcagtc
     9181 catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc ggcaagctga
     9241 gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt
     9301 tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc tgcatctgtc
     9361 ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg ttccaagttg
     9421 tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca aagttcccct
     9481 taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga gaaataatct
     9541 cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg gcaggtttca
     9601 cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt
     9661 catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc acttttttcc
     9721 gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc atcccaagct
     9781 ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca aattccgcca
     9841 acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga tgttttggct
     9901 ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg ataatggatg
     9961 actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg attgtaaaat
    10021 gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg cttaaaagtc
    10081 gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg atatctttta
    10141 atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct ttggatcgtt
    10201 tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta gattcagtat
    10261 cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac cattcggctt
    10321 tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca ttttgagcca
    10381 tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa
    10441 gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc
    10501 ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc gctacttgtt
    10561 ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat
    10621 ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt gtgctgaatt
    10681 ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg atttcccata
    10741 tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta tcttttgtct
    10801 gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa ggtagtttgt
    10861 tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt
    10921 tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct tggctgtcgt
    10981 agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc gtaaaaaaag
    11041 accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca cacaaaaaga
    11101 cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc cttgtctcca
    11161 caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc
    11221 tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg tcaactactt
    11281 cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt tttctaatgt
    11341 ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac ttaagacaat
    11401 tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa tagcgattat
    11461 tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc cgtggatcgt
    11521 ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga tctttttttc
    11581 caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc ccaacaaaaa
    11641 aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc
    11701 ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt
    11761 cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
    11821 tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg
    11881 atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga
    11941 agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt
    12001 gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata
    12061 actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc
    12121 ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat
    12181 ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc
    12241 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
    12301 tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact
    12361 tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa
    12421 gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta
    12481 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
    12541 gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc
    12601 tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg
    12661 actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
    12721 aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata
    12781 tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg
    12841 gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga
    12901 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag
    12961 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
    13021 tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc
    13081 gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg
    13141 ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg
    13201 cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg
    13261 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
    13321 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac
    13381 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
    13441 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg
    13501 gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat
    13561 aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt
    13621 tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa
    13681 atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa
    13741 atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat
    13801 tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt
    13861 gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt
    13921 ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag
    13981 gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa
    14041 attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata
    14101 gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat
    14161 gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg
    14221 gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa
    14281 ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc
    14341 cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga
    14401 attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa
    14461 aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct
    14521 cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac
    14581 gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt
    14641 cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta
    14701 tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta
    14761 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
    14821 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
    14881 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
    14941 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
    15001 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
    15061 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
    15121 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
    15181 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
    15241 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
    15301 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
    15361 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
    15421 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
    15481 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
    15541 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
    15601 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
    15661 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
    15721 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
    15781 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
    15841 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
    15901 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
    15961 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
    16021 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
    16081 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
    16141 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
    16201 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
    16261 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
    16321 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
    16381 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
    16441 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
    16501 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
    16561 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
    16621 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
    16681 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
    16741 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
    16801 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
    16861 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    16921 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
    16981 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
    17041 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    17101 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
    17161 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
    17221 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
    17281 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
    17341 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
    17401 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
    17461 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
    17521 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
    17581 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
    17641 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
    17701 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
    17761 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
    17821 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
    17881 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
    17941 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
    18001 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
    18061 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
    18121 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
    18181 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
    18241 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
    18301 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
    18361 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
    18421 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
    18481 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
    18541 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
    18601 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
    18661 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
    18721 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
    18781 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
    18841 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
    18901 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
    18961 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
    19021 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
    19081 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    19141 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
    19201 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
    19261 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
    19321 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
    19381 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
    19441 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
    19501 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
    19561 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
    19621 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
    19681 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
    19741 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
    19801 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
    19861 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
    19921 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
    19981 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
    20041 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
    20101 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
    20161 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
    20221 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
    20281 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
    20341 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
    20401 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
    20461 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
    20521 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
    20581 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
    20641 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
    20701 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
    20761 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
    20821 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
    20881 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
    20941 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
    21001 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
    21061 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
    21121 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
    21181 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
    21241 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
    21301 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
    21361 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
    21421 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
    21481 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
    21541 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
    21601 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
    21661 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
    21721 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
    21781 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
    21841 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
    21901 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
    21961 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
    22021 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
    22081 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
    22141 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
    22201 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
    22261 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
    22321 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
    22381 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
    22441 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
    22501 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
    22561 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
    22621 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
    22681 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
    22741 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
    22801 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
    22861 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
    22921 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
    22981 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
    23041 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
    23101 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
    23161 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
    23221 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
    23281 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
    23341 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
    23401 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
    23461 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
    23521 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
    23581 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
    23641 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
    23701 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
    23761 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
    23821 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
    23881 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
    23941 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
    24001 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
    24061 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
    24121 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
    24181 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
    24241 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
    24301 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
    24361 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
    24421 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
    24481 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
    24541 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
    24601 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
    24661 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
    24721 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    24781 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    24841 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    24901 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    24961 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    25021 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    25081 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    25141 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    25201 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    25261 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    25321 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    25381 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    25441 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    25501 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    25561 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    25621 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    25681 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
    25741 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    25801 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    25861 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    25921 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    25981 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    26041 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    26101 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    26161 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    26221 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    26281 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    26341 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    26401 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    26461 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    26521 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    26581 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    26641 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    26701 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    26761 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    26821 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    26881 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    26941 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    27001 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    27061 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    27121 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    27181 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    27241 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    27301 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    27361 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    27421 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    27481 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    27541 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    27601 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    27661 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    27721 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    27781 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    27841 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    27901 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    27961 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    28021 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    28081 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    28141 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    28201 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    28261 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    28321 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    28381 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    28441 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    28501 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    28561 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    28621 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    28681 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    28741 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    28801 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    28861 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    28921 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    28981 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    29041 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    29101 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    29161 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    29221 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    29281 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    29341 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    29401 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    29461 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    29521 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    29581 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    29641 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    29701 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    29761 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    29821 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    29881 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    29941 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    30001 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    30061 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    30121 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    30181 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    30241 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    30301 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    30361 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    30421 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    30481 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    30541 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    30601 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    30661 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    30721 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    30781 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    30841 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    30901 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    30961 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    31021 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    31081 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    31141 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    31201 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    31261 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    31321 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    31381 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    31441 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    31501 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    31561 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    31621 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    31681 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    31741 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    31801 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt
    31861 tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    31921 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    31981 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    32041 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    32101 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    32161 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    32221 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    32281 tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    32341 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    32401 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    32461 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    32521 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    32581 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    32641 aaacggtctt tttttcctca tgctttcct
//
LOCUS       DARDHD010000011        32465 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000011 DARDHD010000000
VERSION     DARDHD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.011"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SDA06_000446"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SDA06_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG4227743.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SDA06_000447"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SDA06_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG4227744.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SDA06_000448"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SDA06_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG4227745.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SDA06_000449"
     CDS             4735..5628
                     /locus_tag="SDA06_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227746.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SDA06_000450"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SDA06_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4227747.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SDA06_000451"
     CDS             7565..8422
                     /locus_tag="SDA06_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG4227748.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SDA06_000452"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SDA06_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG4227749.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SDA06_000453"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SDA06_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG4227750.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SDA06_000454"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SDA06_000454"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4227751.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SDA06_000455"
     CDS             11178..11936
                     /locus_tag="SDA06_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4227752.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SDA06_000456"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SDA06_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG4227753.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SDA06_000457"
     CDS             12864..13895
                     /locus_tag="SDA06_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4227754.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SDA06_000458"
     CDS             13892..14659
                     /locus_tag="SDA06_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4227755.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SDA06_000459"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SDA06_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG4227756.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SDA06_000460"
     CDS             16624..16944
                     /locus_tag="SDA06_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG4227757.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SDA06_000461"
     CDS             16944..17294
                     /locus_tag="SDA06_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG4227758.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SDA06_000462"
     CDS             17461..18399
                     /locus_tag="SDA06_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG4227759.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SDA06_000463"
     CDS             18414..19244
                     /locus_tag="SDA06_000463"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG4227760.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SDA06_000464"
     CDS             19288..20313
                     /locus_tag="SDA06_000464"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4227761.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SDA06_000465"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SDA06_000465"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4227762.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SDA06_000466"
     CDS             21391..21831
                     /locus_tag="SDA06_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG4227763.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SDA06_000467"
     CDS             21834..22436
                     /locus_tag="SDA06_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4227764.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SDA06_000468"
     CDS             complement(22495..23601)
                     /locus_tag="SDA06_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4227765.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SDA06_000469"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SDA06_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG4227766.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="SDA06_000470"
     CDS             complement(25149..27494)
                     /locus_tag="SDA06_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4227767.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="SDA06_000471"
     CDS             27773..28675
                     /locus_tag="SDA06_000471"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG4227768.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="SDA06_000472"
     CDS             28888..30663
                     /locus_tag="SDA06_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG4227769.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="SDA06_000473"
     CDS             complement(30729..32315)
                     /locus_tag="SDA06_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG4227770.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
BASE COUNT        10510 a         5786 c         6872 g         9297 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccact
//
LOCUS       DARDHD010000012        29653 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000012 DARDHD010000000
VERSION     DARDHD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.012"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            125..502
                     /locus_tag="SDA06_000474"
     CDS             125..502
                     /locus_tag="SDA06_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227771.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(682..1977)
                     /gene="purB"
                     /locus_tag="SDA06_000475"
     CDS             complement(682..1977)
                     /gene="purB"
                     /locus_tag="SDA06_000475"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG4227772.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1990..3114)
                     /gene="purK"
                     /locus_tag="SDA06_000476"
     CDS             complement(1990..3114)
                     /gene="purK"
                     /locus_tag="SDA06_000476"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG4227773.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3107..3595)
                     /gene="purE"
                     /locus_tag="SDA06_000477"
     CDS             complement(3107..3595)
                     /gene="purE"
                     /locus_tag="SDA06_000477"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG4227774.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3888..5195)
                     /locus_tag="SDA06_000478"
     CDS             complement(3888..5195)
                     /locus_tag="SDA06_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG4227775.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5198..5779)
                     /locus_tag="SDA06_000479"
     CDS             complement(5198..5779)
                     /locus_tag="SDA06_000479"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG4227776.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5895..5992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6351..7643)
                     /locus_tag="SDA06_000480"
     CDS             complement(6351..7643)
                     /locus_tag="SDA06_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4227777.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7640..8263)
                     /locus_tag="SDA06_000481"
     CDS             complement(7640..8263)
                     /locus_tag="SDA06_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227778.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8266..9102)
                     /locus_tag="SDA06_000482"
     CDS             complement(8266..9102)
                     /locus_tag="SDA06_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4227779.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9119..10006)
                     /locus_tag="SDA06_000483"
     CDS             complement(9119..10006)
                     /locus_tag="SDA06_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4227780.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(10014..11249)
                     /locus_tag="SDA06_000484"
                     /pseudo
     CDS             complement(10014..11249)
                     /locus_tag="SDA06_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11932..12615)
                     /locus_tag="SDA06_000485"
     CDS             complement(11932..12615)
                     /locus_tag="SDA06_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG4227781.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12633..13250)
                     /locus_tag="SDA06_000486"
     CDS             complement(12633..13250)
                     /locus_tag="SDA06_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4227782.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13260..14084)
                     /locus_tag="SDA06_000487"
     CDS             complement(13260..14084)
                     /locus_tag="SDA06_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG4227783.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14077..14637)
                     /locus_tag="SDA06_000488"
     CDS             complement(14077..14637)
                     /locus_tag="SDA06_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227784.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14811..15767
                     /locus_tag="SDA06_000489"
     CDS             14811..15767
                     /locus_tag="SDA06_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4227785.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16072..17028)
                     /locus_tag="SDA06_000490"
     CDS             complement(16072..17028)
                     /locus_tag="SDA06_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG4227786.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17132..17734
                     /locus_tag="SDA06_000491"
     CDS             17132..17734
                     /locus_tag="SDA06_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4227787.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17822..19321)
                     /locus_tag="SDA06_000492"
     CDS             complement(17822..19321)
                     /locus_tag="SDA06_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG4227788.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19337..19861)
                     /locus_tag="SDA06_000493"
     CDS             complement(19337..19861)
                     /locus_tag="SDA06_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG4227789.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20159..20908
                     /locus_tag="SDA06_000494"
     CDS             20159..20908
                     /locus_tag="SDA06_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4227790.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20899..21684
                     /locus_tag="SDA06_000495"
     CDS             20899..21684
                     /locus_tag="SDA06_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG4227791.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21653..22369
                     /locus_tag="SDA06_000496"
     CDS             21653..22369
                     /locus_tag="SDA06_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4227792.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22471..23505)
                     /locus_tag="SDA06_000497"
     CDS             complement(22471..23505)
                     /locus_tag="SDA06_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG4227793.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23509..24567)
                     /locus_tag="SDA06_000498"
     CDS             complement(23509..24567)
                     /locus_tag="SDA06_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG4227794.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24787..25935)
                     /gene="nagA"
                     /locus_tag="SDA06_000499"
     CDS             complement(24787..25935)
                     /gene="nagA"
                     /locus_tag="SDA06_000499"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG4227795.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(26011..26847)
                     /locus_tag="SDA06_000500"
     CDS             complement(26011..26847)
                     /locus_tag="SDA06_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4227796.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(27031..28248)
                     /locus_tag="SDA06_000501"
     CDS             complement(27031..28248)
                     /locus_tag="SDA06_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4227797.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(28397..28576)
                     /locus_tag="SDA06_000502"
     CDS             complement(28397..28576)
                     /locus_tag="SDA06_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227798.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            28631..28714
                     /locus_tag="SDA06_000503"
     tRNA            28631..28714
                     /locus_tag="SDA06_000503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28665..28667,aa:Leu,seq:gag)
     gene            complement(28954..>29653)
                     /locus_tag="SDA06_000504"
     CDS             complement(28954..>29653)
                     /locus_tag="SDA06_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227799.1"
                     /translation="GNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNIL
                     FLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWS
                     SSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMI
                     MGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLT
                     VLGICTFIITVINR"
BASE COUNT         8872 a         6308 c         5097 g         9376 t
ORIGIN      
        1 aatgttcgtg ttttatcaga aacgaaaatc ttataccatc aacaagcgtt tacatttata
       61 cttaatgatg gtatattaga attaactaaa aatttggata atgggttcga ggaggcactt
      121 gccgatggaa gatgcaataa aacaaggtca gcttttttta tcagaattaa cagctgaaga
      181 acgttcctgt ctatataatg atttgctgtc gatcaaagac gtttatttat gcaataaaga
      241 tactagtttt tatacattcc gaaaaatccg ccgagcaacc aagcgctgtg ctgaatgccg
      301 taaaaggact tttctttatc agacagaaca caaaggcgaa ctttcaatca gtcagattca
      361 tgaacagaag cgtttcaaaa atcattatcg tatccagatc ttattcaatg gacaagagat
      421 ggaatacgct tttctcgaaa acttgctgca acatctccat aaccagcatt ttagttataa
      481 tagccaagcc atttacgctt aattcctcta ttttttgtat gtaaagaggc acggtaaaga
      541 ggtagccgct tttaaaatca ggttcctatt attgataaga aaatataata aactaaaaga
      601 agcagaaccg ctaacggatc tgcttctttt ttggttattt ttgctattct attttaattg
      661 aattttgcaa ctcactcttc tttattccaa acctactcgt ttgaatatca cgtcaacgtt
      721 tttcaaatga tgatgataat caaaggcatc gtctatttct tctttagata ataccgatgt
      781 gatggttgga tcttcttcta acagcgggcg gaactgcact tggttgtccc aagcgtacgc
      841 agtttttggc tgaacaagat cataagcttc ttctcttgtc attcccttat cgatcaattt
      901 cagcataacg cgctggctat aaatcaaacc aaatgttgca gacatattcc gcttcatgtt
      961 ttcaggaaag accgttagat ttttaacgat attgccaaaa cgatttaaca tgtaattcaa
     1021 taaaatcgtg gtatcaggca agatgattcg ttctgctgaa gaatgagaaa tatctctttc
     1081 atgccataat gtgacatttt cataggcagt gaccatgtgt ccgcgcacca ctctggctag
     1141 accagccata ttttctgaac cgatcggatt ccttttgtgc ggcatagcag aagaaccttt
     1201 ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg gatttttgca atccgcgtat
     1261 ctctgttgca aaacgttcaa tacttgtagc aataagcgcc atcgctgaga aatattcagc
     1321 atgtagatcc cgagggagaa cttgagtaga gatttcttgt ggacggatcc ccaattgttt
     1381 acatacatac tcttcaataa atggcgggat atttgcgaac gttcccactg ctcctgagat
     1441 ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt tcgatgttcc gtttcatttc
     1501 tgaataccat gtggcaagtt tcaatccaaa agtagttggt tcagcatgga caccgtgtgt
     1561 ccgacccatc atcactgtat atttgtgttc ttttgctttt tcgccaatga tttccgtaaa
     1621 acgtttcaag tcttcccgta aaatatcatt tgcttgcttg atgagatagc cataagcagt
     1681 atctacgaca tccgtacttg ttagaccata atggacccat tttctttctt ctcccaatgt
     1741 ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt gttaattttt cgatttccaa
     1801 gatccgatcg atatcaaatg atgcatgctc acgaattttt ttcacatctt cttgcgggat
     1861 ttcaccaagt tctgcccaag cttcatctgc taaaatctcg acttcgagcc acgcacggta
     1921 tttgttctct tccgtccaga tattccccat ttcagggcgt gtataacgtt ctaacatgtt
     1981 ttttctcctc taatcccaaa tattcgtttg tttgatttct tccagcgttt gctggatgtc
     2041 ttctgtcaag atagtgatat gtcccatctt gcgacctttt tttgcttgtt tttttccata
     2101 gaaatggaat tgccagtctg gcttattctc aatcaagcga tagctttcca gcagttgttc
     2161 tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt actttttctc ctaatggcca
     2221 accacaaata cctcgaatat gggcatcgaa ctgactcata gaacaagctt caatagaata
     2281 atgtccagaa ttgtgaggac gaggagctaa ttcattcaca tatagcccgc ctgtttttgt
     2341 taaaaacatt tctaccccta aaacacctga aaggctcact gcttctgcaa tcacacgggc
     2401 aatacgctca gcctcctcga tcacatcctg atcaatcgcg gctggagcaa tcgtttcatg
     2461 aagaatattg ttgtggtgga tgttttccac gaccggaaaa gtcgtgaaat ctccttgacc
     2521 atttcctgcc accatgatcg aaatctcttt ttcaaacggg atccatgctt ctaaaacaca
     2581 ggttccttct cttaaaaggt ccatggcagg agctagatcc gcccgacttt tcaaaacata
     2641 ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa acacaaggat agccgatccc
     2701 gtcaatcgca tcttggatat ccgtcggact gacgatcgtt gcataaggag caatcacgat
     2761 gttattcgtt tccaaaaaag atttttctaa caggcggtct tgcgtaatcg caagcaagtc
     2821 tgtcccttga ggaataaatg acatgggtaa aatcgcattt aacgcctcta cactgacgtt
     2881 ttcgaattca taggtaatga catctgttct tctggcaagc tctttcagcg caaaggtgtc
     2941 atcatagaca ctttcaatgt gccaatctgc aacttgtgcg gctggacaat cgctcacagg
     3001 atccagcaca cctactttaa agcccatttc ttttgcacta atcgccatca tacgacctaa
     3061 ttgaccgcca ccgacgatcc cgatcgttga accaggcaac aaaggtttaa ccaagttgat
     3121 cactactttc tataacagtt ttagccaaca ttgaacgtct ttcagccaat ttagcctcca
     3181 attctaaatc atacatggaa agcatctgta tcgcaagtag tccagcatta attgctcccg
     3241 cttttccgat tgctgtagta gctacaggta ctcctccagg catttggaca atcgaaagaa
     3301 gcgaatccaa accatttaat gttcgtgatt ggaccggtac accgataacc ggcaaagttg
     3361 tttttgatgc gaccataccc ggcagatgcg cggctccccc tgctcctgca ataatgattt
     3421 tgattccttt ttttcgagcg ttttgtgcat attcaaacat caaatctggg gttcgatgag
     3481 cagaaacgac ctttttttca taagacacac cgaattcatc caatatttga catgcttctt
     3541 tcattgtttc ccaatctgaa gtactcccca taatcacaga gatttttgct gtcattgttt
     3601 ttcctcccag tttttctaca ttcatatttt aacaatcact ttttttgatg tcaaagaaat
     3661 ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt tattaaagat gaaaaaaagg
     3721 aatgtgacaa aagttaggtc acattccttt ttatggataa acggtgttca atcagctgac
     3781 cctcggtatt agctcaaaca aacggcaaaa tacgaggaac tatttttccg tttgtttctc
     3841 taacaatcag gtcaaaacgc tgatttcaca acctttcttt tgctctatta cttcttgaag
     3901 gcttcttcca atatttcttc atcttttttc aatccattaa agatcaaatt caatatgata
     3961 gccgtaatgc tactcattac gatgccattt ccagtgaaca tttgcaatgt ttgcggcatt
     4021 tgactgaata atgtcggcgt aacattgaaa cccagcccaa atccaacaga aacagcaaca
     4081 atcaatagat ttttatcatt gctgaaatca acttccaaca gcatacggat tccttggact
     4141 gccaccatcc cgaacatgac taacattcct ccaccaagta ccggctcagg gatgatttgt
     4201 gctaacgcac cgattttagg gaataatcct aaaacaatca agaagaatgc agagaaatag
     4261 atgggttttc ttgttttgat tcccgaaagt tgaaccaatc cgacattttg agaaaagcca
     4321 gtataaggga atgtattgaa aataccgcct aagataacgg caagtccttc tgcacgatat
     4381 ccctttttca attcttcttc tccaacattt ttcccagtaa tgtcaccaag tgcaaaatat
     4441 acacctgttg attctaccat gctgaccatg gaaatgatga tcattagtaa aatcgaccat
     4501 acgtcgaaag ttggtttacc aaaataaaag ggttgtggaa catggaacaa cggcgcatct
     4561 ccaatcgcgc tcagatcaat cattcccatg aaagcagcta agatgcttcc tccgactaaa
     4621 ccaatcaata ccgcgattga acgaatgaac ccttttgcca gcatttgcac gcctacaatt
     4681 aggagaatcg tcacaaatgc aagtagcaga tttgttttgt caccaaaatc agctgctgtt
     4741 gcattacctc cacccatttt ctcgattgct acaggaatca aagtcaaacc aataacggtg
     4801 ataaccgttc ctgttactat aggagggaat agttttttaa ttttagaaaa gacaccagaa
     4861 actaacacaa caaagatccc agaagcgata atagatccgt agatagctcc tactccttca
     4921 ttggttccga tcaagatcag tggagctact gcttggatcg cacatcctaa aacgacaggc
     4981 aagccaattc cgaaaaatcg gttaactgtc aattgcaata atgtcgctaa tccacacata
     5041 aagatatcaa ttgaaatcaa ataagtcatc tgttcttgat taaagcctag tcctgtaccg
     5101 atcaaaagag gcaccgctac tgctcctgca tacatcgcta gcagatgctg cagccctaaa
     5161 accgcagctt ttccattttg tgtttctttt tccacgatta tgcgtcctcc tcaagaaact
     5221 caacttgtcc attcgtcaag gaagcaatac gcgcaagaga aacgactctc aatcccattt
     5281 cttcaagtaa ctgacgtcca tcttggaaag acttttcgat gacgatcccg attccttcta
     5341 cttgcgctcc agcttgctgg cataattcga tcaatccttt tgccgcttgt ccgtttgcca
     5401 agaaatcgtc gataatcaat actttgtctt ctgaagataa aaatttacga gaaatagaaa
     5461 tcgtacttgt cacttgtttc gtaaaagaat aaacagaagc tgtcaataat tcttcatcca
     5521 tcgtcaaact ttttgctttt ctagcgaaga tcattggaac atctaattct cttgcagcaa
     5581 ataaagctgg tgcgatccca gaagcttcaa tggtcacaac tttcgtgatt ccttgttctt
     5641 taaatacttc tgcaaaccgt tttcccattt gttccatcaa tacagggtct acttggtgag
     5701 tgacgaagct gtcgactttt aatacgccct cgcctaatat acgtccgtct ttttgaatac
     5761 gttcaactaa ttctttcact ttatttgctc ctttatcatt tattaatacc gaaataaccg
     5821 cttttgtgca aaaacctact atgtttctgt taaagaaaaa caccctccac tttatcataa
     5881 gaaaagaagg gtgaaatagc caatctttta cttatagtcc agcatttacg gtactggcta
     5941 gaaactgtca tccatattat gacgatatat aagtaggtat gtaaaactaa taatagtgta
     6001 aattcatttt cttgcaactg ttttgcacgt gaaggcagtt tgccatattt gaataaaaaa
     6061 tgttgtttca aatattgaaa acagtctaac actactttct gaaactgaca acgtggttta
     6121 cttatttttt tgtaaaatct aatgattttt aatgcattat tcctttttgt tcgggtttaa
     6181 atagttattt ttattttctc ctatctaaaa aagcagccca cataaaaaga aggcatgaca
     6241 aaaagcatgt catagcccct ttttccgaat aaatagtgtt caaaagacag cctatcggca
     6301 ataaggcaag attttataaa ctacgggaag taatttccgt aaatctattc ttattctttt
     6361 aagctgacaa gctcgtcaat cactactgct tttcctgaag caatagaatg atttgctgcg
     6421 attcctgtca aaatcgacaa aataccatct acgtgagttg ctgcccgatt ccacggatct
     6481 tcttcttttt cttcaccaaa tagatcatct aataaacgcg gatctcctcc gccatgtccg
     6541 cccgcttctt gttcgacgat tacctcatat ggctcaccga acatcggata gacatggatg
     6601 gactgaagaa cggctgctcc ttcatccttt ttatctccac ctgcattgat gtaagacttt
     6661 tcgatcactt tcatttcgat ccgtcctttg ctgccattga aagaaacatt gaatccttcc
     6721 cacggcatat acgcgtttag cgaatacgtc aaaatcgcac cagtttcata tttgaccgtt
     6781 acgcctagtg tatcttcaat agagatatca tcggaaaata cactgcggtc acgaatatat
     6841 ccactgtctt tttctgcttc taaatacatt tctcgcaatt cctcattggc tgacaggtct
     6901 atagcaaatg gatcagcttt agctagttcg cttccatagc atcgactgta aaatatttct
     6961 actccgcgtt tctccgcatt atgcatccca taaaagttta atccaccgaa tgcaaatacc
     7021 ttttggggac ggctgttcaa ccaaaaattc actagatcaa aatgatgggt agatttatga
     7081 acaagaagcc cgccgctatt gtgcttattt cgatgccatc ttctgaaata atctgccccg
     7141 tgttgcgtat ttaacagcca ttcgaagtgg atagaaaaca catcaccaat cacatcactt
     7201 tctatcagtt cctttatctt cgtgtgatac ggtgcataac gataattaaa agtgactcgc
     7261 aggcttcttc ccgtctgttt ttggaccgtt attatttctt gagctttttc ctcatcgata
     7321 gtcagaggtt tttcgcaaat aacatcatat ccttttttca ttgcacgaat gatataatca
     7381 tgatgcgttc gatctactga cgtaataatg acttcagccg gccgtacgtc atccagtagt
     7441 tgctcaaatt gtgatggcat acatgtcttt acttttttct gtccatactt gttcacaagt
     7501 atccgatttg cataatccat ccttgtttga gatacatcac aaaaaccgac tagttcacaa
     7561 gtttgatgat acgtagaggc aattgcctca taaaaaaaac gcgctctccc accaagtccg
     7621 acttgtacat attttttctt catttaaagt cattctcctt ttcttttaag caaaatactg
     7681 tgacaaatgg cagccctgga tacagtaatg cagtgagtaa aaaatcaggc tgtatacacc
     7741 agaacagtcc atacaaaagg cataaatacc acagaaaccc cttgacgttt tttgacaaca
     7801 gccagatatt ccttttgatc aaataaacaa gactatctgc tttatcttct ttgcagatat
     7861 caattagcgt actaaaaaaa ctatgggata caaaaagcaa cagacacaat agaaaaggaa
     7921 tcagatacca agctccaatc tttcccacta ataaatacca atcaactgca aaaataatga
     7981 caaacagctg aaacaacgta atgatgaaac acatccgttt gattttccct ttcctccaac
     8041 ataccttttt ttctgccaag taatggacag atagataata agaaggatac atcatcaaaa
     8101 aataaatcag atagacaaca cgagcagaca gcactaatcc taaagtgaac aaacatccta
     8161 gaactaaagc gaaaagagca ttactgataa gactggttaa aaaaatccga cagacatagt
     8221 agttgagctt gtaaagaaac atctcttcta ttggcttttt catattcagc ccttcagacc
     8281 gcttgtactg atcccctgga caagatattt attgaagaat aagaaaatca gaaaaaccgg
     8341 tagcagcgac aacgtagaca tcgcaaacat actcgcgtaa gtcgtactag atccagcgtc
     8401 tgcaaataat ttgatggcaa tcgataccgt gtaagatctc ggcttgttta aataaatcaa
     8461 tggtcccatg aagtcgtccc atttccaata aaactggatg atgatcgtcg tgatgatcga
     8521 aggtttcagc aaaggaaaaa taattttggt aaagacacta tacttgctgc atccgtcaat
     8581 aatcgctgat tcatctaact ctttggggat gctgaccatg aattgcatga tctggtaaat
     8641 gaaaaatgct tgtccaaaaa aatgtggtaa aatcagcggc acatacgtcc caaccaaatt
     8701 cagccgattg taaataataa actgcggaat caaaatcact tgtgcaggaa tcatcatcgt
     8761 tactaacatg acgacgaacc agaatctttt tcctttgaaa tcgattctcg ataatgcgta
     8821 agcaatacaa gtcgagctca gaactgtccc tatcgtcgaa actgcagaga tgacaaacga
     8881 attcctaaag aaagtaaaaa atgaaattcc agcaaacccc gcccatcctt ttgcgtaatt
     8941 ttcccattga aaagtctctg gaatcaaatt cagcgaacca ctcataatct cttgatccgt
     9001 tttaaaagaa cctgcgatca tccataagac cggataaatc atgactagac ctaatagcaa
     9061 aataaaaccg tattgaaacc atttcgttac tgtattattc atttcttcct cctctcattc
     9121 acacaacgac ccattttttt gatgatttga aaatcagaaa ggtgacagct cccataatca
     9181 acagcatgat ccacgaaata gcactagcat accccataga attaaaagaa aaagcttggt
     9241 tatacacata aagagcataa aagttcgtgg catcattcgg accaccttgg gtaatgataa
     9301 atgcctgcgt aaaagtcata aacgccgcga ttgtctgcat aaccaagtta tacaaaatga
     9361 tcggcgaaag aagaggtaac gtgatcttgt aaaacgtctg ccatttgttc gctccatcga
     9421 tctctgccgc ttcataatac gagcctggga tctctttgat acctgcagca aagatgatca
     9481 ttgccgaacc gaattgccaa gcagacatca aaacaagagg gatcatgacc agtttttgat
     9541 cgctaaacca ggaaagcgca ggtgcgccta atcgttccag gaaaatattc actaacccgt
     9601 ttttagaaaa caactcccgc caaactagag cgatagaaat actgccaccg atcaaactag
     9661 gcacataata caaagaacga aagaaagtcg ttcctttgat attcctcgtt aaaagataag
     9721 caatcaatag accaaagatc agtttcacag gtacagatgc aaatacatag agaaaagtga
     9781 ctcggatgga gttcaaaaaa cgggcatcac tcagcatctt tatataatta tccaagccga
     9841 tccatgagac tggatttccc aaacgataat cggtgaacga ataataaagt gacaagatca
     9901 tcggtatcac tgttatcaat agaaatccta ggatgaatgg agaagcaaat acgtaaccga
     9961 ccacattgtc ttgatccaag aaccgtttta gtttttcttt tttcatgcct tccttctgct
    10021 gttacttctt cataaatgac tttttcaact aaaagattgt attggtcttg aatttccgga
    10081 ttcaaagcac tatcaaagac attcacttcg ccgttgtcca tatcggaaat catttctaaa
    10141 taatcaaatg tcgcttgcaa agttgcatca tctgcacgag cgagctgttc tcgaatattc
    10201 gacatgattg gtacaccacg ttctcctttt aaaatttcat tggcttcttc actgttttga
    10261 aagaaatcaa taaacttagc agcttcttct ttatgggctg agctttttgc catactcagc
    10321 atttgcgaag aacgtaaagg gataccagat tctccatctg cttttctgcg aggtggatta
    10381 atcaactgaa tctccatatc ttcaggagcc cctgacaata tttctgtcaa ctggttactt
    10441 gatagaaaca ccattgccgc ttctctatcc ctaaccgcct gaaaagacag atccgtcgta
    10501 cttgcagctc caccacctgg ttctggatgg gcccctttgt ctaccagatt tttacgcagc
    10561 tggaaaaaat ctgttagata gtctggattt tcaaatccca agctgttaga tagatcttcc
    10621 cgattataaa aattcaatcc tttctcaact tgtggtattt gaataataca gccagctata
    10681 aaatcttcga atcttgaaat agcatagatt cctaattctt cagtgatttg atagctgatt
    10741 tgttcaaatt catcccatgt ccaatttgaa gctggttcag ggatatttaa ttgttcaaat
    10801 atatttttat tgtaagcaat cgcaaacgaa ttcaccccgt tagacagtcc aactaattgc
    10861 ccgtcgtaag tcgtcgttgc taaataggat tcatctgcat tagaaatatc aattatccct
    10921 tgctccacat attcatctaa tggttcaatc tgattgatgt attgaggaaa ttgattgttc
    10981 agttggaaaa tgtcccagat atcatctgat gctgctagtg tacctaattt tgtgtaatat
    11041 ccttcataat cataaaattc ataatcgatc gtcacatggg gatttttttc ttcataaagc
    11101 tcgattactt ttactgttgc atcgtgtcgg acttgcgaac cccaccaagc catgttcagc
    11161 gtgatttttt tgtcatcttc ttgcccacaa gccgtcaaga agaataacat aacacttaaa
    11221 gctaagatga ttctcattct tttcttcatt ctctgcacct ctacttccat tgttattttt
    11281 caatatactg tatttttata cagaacatat ttattaaacg atctttttca aggggaataa
    11341 aaaccaagac ttgccttatc gttctgccta ggatgaacaa ctcaatactt tcaagaaaaa
    11401 ttagatagtg ctgattcttc tttcatcgct aaaaacactt cttgcttcaa tgatgggagg
    11461 ataattacgc aaagaaaaaa ggatatcaga caactgccca atatccttga catagctaaa
    11521 taagcatgct tcttcgtgtg cgctcttccc taaaaaacag aaaaataact agtaattctg
    11581 tatgaaatta ggtacaatat acacgtcgat gcacaagcat tgactatggc aacttgattg
    11641 gtcagttccc gctgactttt caagccccga caatttagct gctactaaat tgtcgggtgc
    11701 cttttttagt ttttattcgt ttttcattct atagaaagcg cttcaataaa tcaagacatt
    11761 tttttgttag aatgctaata ataacaaaag gttttaactc ttcttatgta cattacaggt
    11821 aaaaaaacaa aaaaatttct cttttttttg aattctgaac aaatagtact ggagtacaaa
    11881 agacgaggaa gagccactat ctgctcttcc tcgtcttttg attctcacct gttattcatg
    11941 attctttttc tcagtttccc gatatttttc cattttacga ctaaaaagct ctccgctact
    12001 tggtggcgta tatgtgatgg catttgctcc tgcttgaatg gtctcagcaa tactttcttc
    12061 cgttggacca cctgtcgcaa tgataggaag ttcaggatat tttttacgaa aatggcgtac
    12121 tgcttctgca gtttctttac ctgcactgat attgataatc gaaataccag ctgctaactt
    12181 ttcatctatt tctgtatatt tcgacgttac cgtactgata actggaatat caactactcg
    12241 acaaacttct tttactgtct ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc
    12301 ttgcgcttct gcgaacatgc tcatgtagga agaacgagca ccttgtgtca aaccaccacc
    12361 tacaccggag aaaactggaa tgtcagctgc ctcaataatg cttttagtaa tcgctggatg
    12421 aggtgtaaaa ggatacacag caattactgc attcgcattt gtattacgta tgatcgcaat
    12481 atccgtagta aagatgatcg aacggatcaa ttttccaaat atcttgatcc cgcttgcttc
    12541 taaaatgatt tccggtactc ttacgatatc ttttctcaat tccgtcatga tttctggcgt
    12601 aaattgttct gccatcagcc acttctcctt tgctatctcc ggtcatactg attcaattga
    12661 tacgtctcga tgacctggat gattttttgc gcataaccgg ggtctgtcgc ataacctgca
    12721 tcttgcaatg cttgtgcggc ttcctgatag ttggtggccg taatcacttt ttcatacagc
    12781 gcaggattcc aatctactcc ttggacgaac agttgtgtat ggtcatccat ggactgttcc
    12841 catgaatcat acactttgaa gtctccttgg atagttatcc attcttcgtt aacaaattcc
    12901 tttgtttcca ggcttacttt cttctgatcc ccataagcct tgatcccgaa aagattattg
    12961 tatttgctag ccaaggtact ttttccccaa tttgattcta atatagcttg tcctaaaatg
    13021 atactaggaa gcactccata accatcttgt agttcttggg catgcggaac aagccgttca
    13081 ataaattgct gatgggattg cgcttcattt tcttgctgat tatcagtaaa aggagaagac
    13141 agattcttca aggaaaaaac aaaggctaag agtatcactg ctacaccggc aaccactgct
    13201 ggccaacgga tattcttgcg cttttgcttt ttccactttt ttttcgccat gatcaattcc
    13261 tacttttcat ttaattcatt cagatattct tctagcgtca attggtgttt ggtgatatac
    13321 tcagcgtttt ctttaccgac ataacgtaag tgccaaggtt catatgtgat cttagttata
    13381 tcttcgtgtc cttttggata acgaacgata aatccatact gcgcagcatt atctgcgatc
    13441 catttagctc caggctgatc tccgtaactt gcatccaata cagtagaagg ataactgttg
    13501 taccagtttt gatcgacgac atcgactgcc agacctgtgt ggtgttcgct gtatcctggc
    13561 tccgtaatcg tttctttagt aatctttgtc gcctcctctt ctgatacacc ttgacgactc
    13621 atcacatctt ggacattttg ttcgaaaact tgttgttggg atgatacaga ccggtaagct
    13681 gagaccatca ctaatggaaa tccagctgct tcagcagcct tggcaaatgc ttcatattct
    13741 ggagcaatgc gtttgtcgat caagtatccg ttacttaatg atgccaattg agtagactca
    13801 tcaatttctt tttctaattt gtgatctgga ccaaccaaaa ctagcttcca atcgtctgtt
    13861 ttaacatctg gcaattgttt ggacgtacga tcttttgttt ctttgtcctt taccttgctg
    13921 ctggttgttt gactgttctt ttctttacta gcagttgttt tggtgcttgt tcgttcgact
    13981 gcttgattgc cggcttgaac atgcgaatca tcatcgctaa aaataacata gcccattgct
    14041 gcgatcataa tcacaactgc tccaaatcct aaaaatttat tcacttgaaa aatcctttcg
    14101 ttgttcttca cgttccccat tcgcctcatg aacgtaagtt tcttccagat tttcattcac
    14161 ccaatcgaga agttcgattt tttctccttc gatctgtttt tgaacatctg ctggcagacg
    14221 aaaattcaaa atatcatcat agccccattc atcatattga atacttactt tcaagtcaag
    14281 gtatctacca gcctgctgat tatttttcaa agggatcagc tcgacttttg ctgctttgct
    14341 gctaagccat ttttgcgcga tgcgtgtttt gatttgcttg tactctgcaa cggcaacgtt
    14401 tcgtttttcg ggaaaaataa tcgtttgacg caaaatcttt tgcgttagca tccattcata
    14461 atcagtgaaa agatttttta ctttctgcat cgcttcagat ggcagatttt tctcgccatc
    14521 tttccatttt tgccattgtt ctggactgat cagcaagtat tcatctaaaa aatttttttc
    14581 agtttcaaac tgttctaata atacactcat aataagatca atatatactt gatgcacgtt
    14641 tatcccctcc ttttaactat tctacttaag ttttgatcaa aaagcacctc ttaaatcgct
    14701 gctttcattt tttttaatgt ttcaagtcct ctcctcttta ttcttcgtga tttttgttat
    14761 aataatagac agacaagaaa ctcaatccaa ctatgaagga gcgttttcag atgaaaaaat
    14821 atcaatgggg aatcgttggt cttggtacca tcgcacatga gtttgccgaa agcttcaatc
    14881 aagagacgag cgaactaaca gcagttgctt ccagaacttc agaaaaagcg gaaaacttcg
    14941 ctcaccgtta caatattcca aaagcttacg gaagctatca agaaatgtta gacgatgctg
    15001 aaatcgacat cgtgtacatt gctgtaccaa atagacagca tatcgaccat atcttggcag
    15061 cattggaagc aggcaaacac gttctatgtg aaaaagccat cacgatgaat aaaaaagaac
    15121 tggctgacgc tatgcgctta gcagaagaaa agaatttgat cttagcagaa gcgatgacaa
    15181 tcttcaacat gccactttat cagcagttac gctcaataat ggatacagga aaactggggg
    15241 ctttaaaaat gatccaagca cctttcggca gttacaaaga accagaccct aaaaaccggt
    15301 tcttcaatcc agaattagct ggaggagctc tgttggatat cggtacgtat gctgtatctt
    15361 tcgctcgttt ctttttgagc tctcaacctg aagtagttgc ctctacaatg gttccttttg
    15421 aaacaggcgt ggacgaacaa tccgtcacta tcttgcgtaa caaagaaaat gaacttgctg
    15481 ctgtcagctt gacttttcag gcaaaaatgc caaaagtcgg tatcgttgcc tttgagaacg
    15541 gttatgtgac gattgctgat tacccgcgtg ctgatcgtgc ggagatctta tttacagatg
    15601 gtacaaagga attcatcgaa agcgggaata catctgaagc aatgaattac gaaataaaga
    15661 atatggtaca catgattgaa ggtgacttgc ccaatcgctc tctcttcttg acaaaagatg
    15721 tcatcgaaat cttggatcaa atgcaagttc tatggaaaaa tatgtaattt taaaaggttg
    15781 tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc tcctcctatt
    15841 tttcgtttgg ctggacttat taccgcagag gaaagctttt gaacaccgtt taagaaagag
    15901 gttgtgagcc taccgttcag ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    15961 tgtttgttga atattgtgac ttgtgaccga ggagttgaca ggagaacacc gtttaaagaa
    16021 gaggttgtga aatcagcttt ttgacctgaa tattagctga attcacaacc tctatttttg
    16081 gattttttgt tgggagaatt ctgccttgat ttcaataaaa cttttaaaag actgcaaaaa
    16141 ctggaacaat tctgctcgat cctcgaattc agggcgacta gtcggcagag gcatttgccg
    16201 atacaataca taaacttctt caatacgtgt taaaatttgt ctgccatcat ttgactctga
    16261 aaatgtttct gcagtataaa tcaataaacc atagatatgt tttccataat aagctggttg
    16321 ctggattctc tctagatttt ccagcatgtc tttaatcaca ttggcctgtg ctcttctcat
    16381 agagaagtaa gtttcgtagt atagcggctg tctgagccag tagttttctt gatgttctct
    16441 ggcatcttct tggctttcgc gaatgaatgt cagtaaatgt tcgcatttga tctgcacatc
    16501 ttctttattt tctgataaaa gaaattctgc catttgtctc aaaatatttc gaaattcttt
    16561 ttcgatctgt atttggtttt ccttcagctg cttcgtacga tctggcatgt aaacatttgc
    16621 aagcaaagca aatactaatc caatcgccat taatcccgct tcattgccga tcagctgcca
    16681 agacatgttt tcctccacta aataatgagt gaccaaaacc gaatttacta ctatcccttc
    16741 ggttaatttg aagcgagagg caagcgggat gaacaggaat aaaaaaagac tgaagccaaa
    16801 aatagtatac ccaacagcaa aaaaactgaa cagtgctgtt gccgtagcta atataaaggc
    16861 tgcgattcga ttcatgcccg tacgcaatgt agagcgcttg gtatttccta cacttaataa
    16921 tgcaatgatc cctgcagctg gccaatacaa cagcgataag ctacccgctg tgaccatcga
    16981 aaagaagaca ctgatcactg ttttgactgt tcgcattcct atacgcattt acttccccct
    17041 tttttatttt aattatactt gaataatgaa aaactagcta tcagacaaaa gtttaggtat
    17101 gatagtaaaa agaccaaaaa ggagaatctc tatgcaattt ggccgttttc gactaggtat
    17161 gcgtacgatc aaaagtgcct tagctgtttt tttatgtatc ttgttttttc atttgactca
    17221 acgaggaatc ccgatgattg ctgcattatc tgctgttttt tctttgcgtc aggacttgac
    17281 tacaaccgtt tcttttggaa agtctaggat catcggcaat acattaggtg gtacattagc
    17341 tatcatttat ttttttgtca aaagctattt tcacaatgat tttttggtag aattgatctt
    17401 gcttcctatt ctagttatta ttgtgatcgt cgtgtcagat ggtattaaca ataattccgg
    17461 tatcatttcc gcaatagcta cgttgttgct gattgcgttg agcatccctc aaggcgaatc
    17521 cgctttgtat gctatccaac gtgtactaga tacttttata gggacattca ttgcaatcgg
    17581 tatcaatttc tttcttcgtc ctcctgaaac agaaaagaag gaagaactat cagaagattt
    17641 ggtcgaactc caaaagaaag aacgattgct aagaacaaat ttacaagaga tagaagctaa
    17701 gatccaaaaa caaaagaaaa atagcgagga ctaattttct tttgcaataa aataaaaaaa
    17761 tggagagaag aactttaaaa agtcttcctc catttttgat tgaacggtga agtgtactac
    17821 tttattcttc cttcatcgag ttttgttctg cctcttttgg cgcttcatac atatttactt
    17881 ggctagtagt cccacgtgtc gacaaattct ctacgatttc cttcaagtca agtcctgttg
    17941 tcgcttttag tgtttcttga gtagaagaaa gcaaatttgt ggcataattt gtcactcggt
    18001 tcgctccact ggtttctcct gaactgcttc ctgtatcgac aacagagatt ttttcgatat
    18061 ttcctagcgg ctgtgctgct tcgcgcatca attgcggtag catatcgata accatactca
    18121 agacagctgc ttcgccatat tccttgaagg cattggcgat cttttgtttt gcttcggctt
    18181 ctgcttttcc ttttgctagt gctgcttcgg cttctgcttg tccagctaaa cgtgtcttat
    18241 tggcttccgc ttctgccagt gcttccacac ggaatcgttc tgcttcggct tcagtcactt
    18301 ctttgacttt ttgagcttgt gcttcttgtt ctttagcgta ccgatcggca tctgcttttt
    18361 tcttcacttc cgaatcatat tgcttttcac gacgtgtgat ttctttttct tccagttcga
    18421 tttgtttttg gcgttctacg attttaatct gcatttcctg ttcgattact tgttgttgtg
    18481 ctcgggcact ttctaaattg taggcttggt ctgcttttgc tttagcaaca tcttgttctt
    18541 gtttgtaggt tgctaatttc agttcttttt cttttaatga ttctgcgatc tctgtttgtc
    18601 gctgtaattc agctgcttgt gattcttttt ctgcttgagc ttttttgata cgcgtttctt
    18661 ttagagcttc cgcttctgct atatctgcat ctcttttgac ttgggcgatt cttggtttcc
    18721 ctaaagagtc aagataaccg tttttgtctc tgacctcttt gatagtgaaa gaaacgatga
    18781 tcaaccccat ttttgcaagg tctacagaag cgacttcttg tacgctttga ctgaacttat
    18841 cgcgattttg atagatttct tctacagtca tagaaccaag aatcgagcgc aagtgtcctt
    18901 ctaatacttc tcttgcttca ttttccagtt cttctcttgt cttgcctaaa aactgttctg
    18961 ctgctgtagc aatctcttct acagatgaac cgattttgat gatcgaagta ccatcacaca
    19021 tcaccggtac gccttgttcg gtatacactt caggggtgga gacgtccaat ttactcgata
    19081 atagactgat ccgattcgaa cgttgaaaaa ctggcaagac gaatgcgcca cctccacgca
    19141 caattttgat tttgttattg ctttcatcaa tgtgcacatt tttgcttcct aaataacttc
    19201 cactgatgat caacgcttca tccggttttg ctgtctggta cttcgctaca aagacgatca
    19261 gcaacatcag taaaatcaaa gctgcgattg cgatcgcgat aaaaacaact ggtatttcca
    19321 tttaacacac gctcctttaa gcttttatca tgctttcata aggcatgaca taacagattc
    19381 tttccttgat ctcaatgatc aatactttcg ttccccgctc agctgtctgt tcttgggggc
    19441 tgtaaaaaca agctggacga ctaatggaac cggtaacact tttcaactga atttctccca
    19501 tacctcgtac agggatcggt gtgatcacaa cagctacttg tccttccaga gttttttcag
    19561 aagacgacat cgttgattcg gcatgtttca tcggcatcaa tatatagaaa ttaagcaaaa
    19621 ataccaacac tatcgatatg cctccactag caataaataa aaataaagat gattgatctg
    19681 ttaaacgctc acctagaaaa ccaaataagc ttgtgaaagt taaccaaggg ataataagca
    19741 tcggatcaag cgggccatca aaatcgaata catctccaaa aataatcaat aggactgcca
    19801 atcctgcaca aatcagcaat gtataccaat aaatcgtctc aagtgcgatt ccccctatca
    19861 tatacacgcc tccttccatt tctacgtcta tcataccaat gtttgaaaat tctgtatagc
    19921 ttcttttacc gactcagtgg tgttttttat aggaaggttg tgtttatcgt tgacaatttg
    19981 ttaaattttt gttttcatgt taaaattttc acaataaatt ctttcgaggt agcctatgtt
    20041 tccttttttt aagaaaaaac cattaaagct ccagaacacg atcatccttg tcgtttgttt
    20101 ctccacattc gtttctttga ttgtttctgc catattgatc aggaatttta ttctttcgat
    20161 gaaccgatct agtgtgtcag ataaaatctc tagcattgct aaaatcgttg ccaatgataa
    20221 actcatccaa aaaaagctgc agactggtga cccgacacat gcgattcaag gaataacaaa
    20281 tgaaatcatg attgcatcga atttagattt tatcgttgtc atggaccctg aaggcattcg
    20341 atattctcac gcagacccaa gttacatcgg tcttcctttc tcaagtcatg aagatgtgaa
    20401 aaaagtgatt ggaggacaag aatttttctc tgaaaaagaa ggaatgttag ggttcggctt
    20461 ccgctatttc gtccctattt attatgatca agagattatt ggtgcagttt gtgttggctt
    20521 aacaaacaac acgattgaca atcaagtcaa agatggacaa aaaatgattg ttttttcatt
    20581 gattattgga cttagcgtag gcatcatagg cgccgttttt cttgcccaaa aaatgaaaaa
    20641 agtactgctt ggacttgaac cacaagaaat agcagaacag ttaagtgaaa aaacgattat
    20701 cgaaaatgaa gtagatgaag gaatcttagc catctcttct gataaaacta tcctcttgat
    20761 caatacacct gcaaaaaaca ttttctacca aacagagcag ctattggatt tcgaagtagg
    20821 aaaaaaatca gtgataatct atatgacgtt ttgtttaaag agacgattga gcatcaaacg
    20881 accgttcgga ctcaatccat gtatttaaat gggatagaaa ctatttctag cgttacacct
    20941 ctttttagca atgaccgttt tttaggagca gtcgcaactt tacgaaatcg atcagaagta
    21001 atgaaattaa tggaggagct tggggggacg aaacaatata taaactccat gagagctcaa
    21061 acccatgaat tcatgaataa acttcatgtc atttctggtt tgattgattt gaagaagtat
    21121 gaagaagtca cccattttat ccagcagcta aatcaaaatt ataaaaaaga gattggccaa
    21181 gtctcaagtc tgatcaaagt acctgcaatt gccggttttt tattaggcaa aataaacgaa
    21241 gccaaagaac aatctgttga aatgctgtta gaaagcgatt cttttatccc taaaatggag
    21301 atggatgact ccatccattc acttttgcaa gtgttaggga acttacttga taatgcaaaa
    21361 gaagccgtgc tacaaaaaca tgaaagaaac ggacaggtcc ggattcattt aaactatgac
    21421 acagaagctg aagtattcat cgtgaccgtc tgcgatggcg gtgtcgggat atcagaagaa
    21481 atctcagaaa aaatatttac gctaggtttt tcgacgaagg gaatcaatcg tgggtttgga
    21541 ttgaatctca tccattcaat cgtcaaacgc cacggtggat ttttagaatt ttccaaacaa
    21601 acaaatggcg gtacaatcgt ctatctagaa ttaccaaaaa aggaagatca aaatgatgac
    21661 aaatgtcctg ataatcgaag atgatccaat ggttgcagaa ttgaatcgta cttttgtgga
    21721 aagcatccct aatatgaacg taatcgaaag tttcagaaat gtagaagaag gaaaaaaata
    21781 tttcagacaa aaaaacgcag atctgctgct gcttgatgta tacatgcctg gcgaaagtgg
    21841 tatccatttt ttatcttggc ttagacagca aggaaatgat atcccagtga ttttgatcac
    21901 tgctgcagat gatatcgaaa ccataaagaa tgtgatccgc tacggtgcct tagattattt
    21961 gatcaaaccg ttcactttgc aacgttttca gctggcgatc gaaaagtatc ttgctttctc
    22021 tcagacaatc caaacgactg aaaaagccga tcagaaattg atcgatcaag tattcaacca
    22081 caccactccc attcacgaaa taccatcatt catcgatttg cctaaaggtc tgtcaaaact
    22141 gactttaaaa aaaataagtg catttattct aaaagaagat actatttttt caacaaaaga
    22201 actggctcaa aaaagctcac tgtcacgtat atccgtcaag aaatatttga attttttagt
    22261 agaacaagat atattgaacg aaagaatcaa atatttggaa atcggccagc cgctaacctg
    22321 ttatgaattg acaaagaatg ggcggaaaaa gctcacagat tactgttgag tcatcgaatt
    22381 atcccataca aaatagtcca agtatacaga agtaacaact gtttacttgg actattttta
    22441 aacattttat tgacgaatat ccatgttttc ttatcttaat tgacgggctt taggaagaga
    22501 tacctcattt ttcgtttttc cagtttctat tatttcacga aagttctcga aacttgtcga
    22561 aatcatattt gataatgcct catctgtatt tgaacctaca tgcggggtta caagaactct
    22621 agggtacatt tctactaatt gctggatagc agggtctgga atcgtctccc atggcttgaa
    22681 ggatcggaaa aaaacctctg tttcatttgc aaaaacatcg gttgcaaaac cagctaaatg
    22741 attgcttttc aaagctctta aaatagcttc attatcttga agctctccac gggctgtatt
    22801 aatcaagatg ctaccttttt tcatttgagc taaaaatcct tcattgatca tttgatcgtt
    22861 ttcacctggg atatgaggta catgaatact gacaatatcg cttttggcta ataactcttc
    22921 aagcgagtgg aacgtcaata cttttttagc agcttctgtc tgatgaatat cgtagccgat
    22981 cactttggct cctagtccct taaacaaaac agcttccgtc aaccctatcc tgcctgttcc
    23041 tatgatccct actgtgcaat ttctgatttc acgactaaac atttgctcat ccactcgaaa
    23101 atctttgaaa gatgttcgga tcgtcgtata cgcagtattt ctcaaaagca tcatggcaaa
    23161 agtcaaagat aactctgcta tcgcatttgg cgaatacgat ggtacacggg ctactgtcat
    23221 accaaaatca gctgccgctt gtagatcgat atgattgact cccacagtcc gtgtaaaaac
    23281 ataacgaatc ccatattcct gcatttttgc aagattggtc tcatctgcta tacaatttcc
    23341 cctcaacaat acagcatcat ggtcctttgc tgacacgata ttgtcatgtg tcaacagttc
    23401 ttcataaaga ctgagatcat aatggtaaat attcaatttt tcaaaaaaat ctatctcgtt
    23461 cggacgaaca ccgtaacatg cgattctcat actttcttcc tccatttctt atgaaaataa
    23521 acctatgcca tgaatcattt gtaatacaac gatccataca ggtgtcagta ccacagcaaa
    23581 aatagttgac agtaaggatg cattggaggc taaaagtgcc tccctatcaa aactgatagc
    23641 ataggctgct gccactgttg cagctggtgt agccatcatg ataacgattg ttgccagtgc
    23701 ttcaaaattg actggtagaa tattcgttgc cgtcaaaact gctagaatca cgatattcaa
    23761 tgcaggtaca aataccactt tgactagact gtaataccat gaagtttttt cagatgcagc
    23821 ttttttgaag cttacttcac ctaatgttgc gccgattgct agccacgcca atggtgaact
    23881 taaaccagct aaataatcca tcggctgaaa tagccacggt gcagtctggt caattcgcaa
    23941 aaacgctact tggcttatct ctccttctgc acctgtaact gacacttgtg gcagataccc
    24001 ttggaacagc caaatgaaaa ggccggcaaa agtagcgata atgattggat tgacgaacat
    24061 cgctttgata tttttagctt ccatcttcaa gccactcatt tttatgtatc cataagaata
    24121 caaaaagatt cggtagccaa tattgaaaat tgaggaatac atcacgccga caggaccata
    24181 aatcgcactg acgataggcg ttccgaaaaa ggtcgtagaa ccaaagatcg tcaggacacg
    24241 caacgttgtt tgcttatccc ctttatagct taagaaaaaa ggttttgaaa ctagaataag
    24301 aataatataa ataactaatc cccaaatcaa gacattgatt ccttgcatca gtaattctgt
    24361 attgatatct tgcatgaaag ctcgaaatgc cagtgctggc aaagccactg ttaagacgac
    24421 tttcgataga atttttccaa cagattcaga aaaaatcccc ttttttctac aaacaaaccc
    24481 taataatata ataaaaactg ttgaagtgat tgcactgaca atcgacatat ctgtcaaagt
    24541 cgatcgaaaa atgtcaaata agttcatctt tctcctccta attatgttca tttattcaca
    24601 ataaaaaaag cttttatttt gctaatacgt attaacattt ttaattttat ctttacattg
    24661 ttacttattg aacaatagga aattaaagaa acttttgaaa cttatgtaaa gtgattttta
    24721 ctagaacaca aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa
    24781 attatattat gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc
    24841 agggttcaat acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc
    24901 gattttacag ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc
    24961 taatccccag tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa
    25021 gttgccagat tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt
    25081 gtaatttcca tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc
    25141 atgtcctgct ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca
    25201 aatcaattca gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg
    25261 atgattcaat ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac
    25321 agctttttgc gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc
    25381 ttcagtcact tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt
    25441 tttgattaaa ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc
    25501 gaaataactt gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata
    25561 gattcctttg attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac
    25621 atcttttaaa cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca
    25681 agataacaga ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg
    25741 attcatatag ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg
    25801 gtcaatgatg ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc
    25861 ggtaatttca agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata
    25921 gataaaagtt ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt
    25981 agagaagaag tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc
    26041 ttgtgctaag ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa
    26101 gcttttaagc acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata
    26161 gattccttga ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt
    26221 tccataatta taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg
    26281 cagcagttgg atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc
    26341 atatattttt ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc
    26401 tttttggcta gagtagctta atgcttcttt caaataataa agggactgag agatattttg
    26461 acgcaacgtg aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg
    26521 atacagattt tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg
    26581 tttttggaaa caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg
    26641 agagagattc atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac
    26701 caatacattc ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat
    26761 atcatgcgaa agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt
    26821 tccaaaggac tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt
    26881 ctattcgtat tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat
    26941 atactttagt tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg
    27001 ccctcctact gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc
    27061 tctcattttc ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa
    27121 atgatctggc tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg
    27181 taaaaagtcc aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt
    27241 tgggaatata taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat
    27301 tgcattatag atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc
    27361 gacactttga taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca
    27421 caaacgcatg gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc
    27481 tccacttagg accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc
    27541 gttcagcgta acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc
    27601 atccatcact aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc
    27661 tacttcaacg atttcttcta ataattcttt tggataaagt gccccagtag gattattagg
    27721 attaatgata acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc
    27781 aggataccat tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc
    27841 aatagaagcc gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc
    27901 cagcaacccc tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc
    27961 attgatcgtc acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc
    28021 agaaaagatc cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc
    28081 acgaatgact tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt
    28141 taaaatacta atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac
    28201 atcgtaactg acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat
    28261 ccctcatttc aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat
    28321 actccatcct agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca
    28381 aacaagcgtt gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca
    28441 ttgcaattcc acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc
    28501 caatcaatac acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct
    28561 taataagtcc tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg
    28621 ttattattac gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg
    28681 ttagctcgtg tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct
    28741 tcacgagttt ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt
    28801 tacaaaaaga acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca
    28861 agctactcgt agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct
    28921 cacctaatac taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa
    28981 ggtacaaata ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat
    29041 gcttttcatt agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat
    29101 atagtacgtc cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga
    29161 acaaaatccc attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact
    29221 taaataaatt ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca
    29281 agtacctgga taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt
    29341 actactactc caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat
    29401 tgtattacca aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac
    29461 ataatagttt tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa
    29521 taagatattc atgtctaaaa aaaacggctt tccaaatgat gaaataccta aatctaactt
    29581 attaagaaag ttgaaatttt tatattcagg aaaccattta ttaatttcat gtgcagcacg
    29641 taatgcattc cct
//
LOCUS       DARDHD010000013        28377 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000013 DARDHD010000000
VERSION     DARDHD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.013"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(319..1014)
                     /locus_tag="SDA06_000505"
     CDS             complement(319..1014)
                     /locus_tag="SDA06_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG4227800.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1104..2204)
                     /gene="ychF"
                     /locus_tag="SDA06_000506"
     CDS             complement(1104..2204)
                     /gene="ychF"
                     /locus_tag="SDA06_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG4227801.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2213..2404)
                     /locus_tag="SDA06_000507"
     CDS             complement(2213..2404)
                     /locus_tag="SDA06_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG4227802.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2425..3315)
                     /locus_tag="SDA06_000508"
     CDS             complement(2425..3315)
                     /locus_tag="SDA06_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG4227803.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3302..4069)
                     /locus_tag="SDA06_000509"
     CDS             complement(3302..4069)
                     /locus_tag="SDA06_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4227804.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4081..4794)
                     /gene="rsmG"
                     /locus_tag="SDA06_000510"
     CDS             complement(4081..4794)
                     /gene="rsmG"
                     /locus_tag="SDA06_000510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG4227805.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5026..6927)
                     /gene="mnmG"
                     /locus_tag="SDA06_000511"
     CDS             complement(5026..6927)
                     /gene="mnmG"
                     /locus_tag="SDA06_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG4227806.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6944..8341)
                     /gene="mnmE"
                     /locus_tag="SDA06_000512"
     CDS             complement(6944..8341)
                     /gene="mnmE"
                     /locus_tag="SDA06_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG4227807.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8640..9023
                     /locus_tag="SDA06_000513"
     CDS             8640..9023
                     /locus_tag="SDA06_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227808.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9191..9949)
                     /locus_tag="SDA06_000514"
     CDS             complement(9191..9949)
                     /locus_tag="SDA06_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG4227809.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9967..10785)
                     /locus_tag="SDA06_000515"
     CDS             complement(9967..10785)
                     /locus_tag="SDA06_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4227810.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10782..11138)
                     /gene="rnpA"
                     /locus_tag="SDA06_000516"
     CDS             complement(10782..11138)
                     /gene="rnpA"
                     /locus_tag="SDA06_000516"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG4227811.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11256..11390)
                     /gene="rpmH"
                     /locus_tag="SDA06_000517"
     CDS             complement(11256..11390)
                     /gene="rpmH"
                     /locus_tag="SDA06_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG4227812.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11913..13247
                     /gene="dnaA"
                     /locus_tag="SDA06_000518"
     CDS             11913..13247
                     /gene="dnaA"
                     /locus_tag="SDA06_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG4227813.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13445..14575
                     /gene="dnaN"
                     /locus_tag="SDA06_000519"
     CDS             13445..14575
                     /gene="dnaN"
                     /locus_tag="SDA06_000519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG4227814.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14804..15046
                     /gene="yaaA"
                     /locus_tag="SDA06_000520"
     CDS             14804..15046
                     /gene="yaaA"
                     /locus_tag="SDA06_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG4227815.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            15033..16157
                     /gene="recF"
                     /locus_tag="SDA06_000521"
     CDS             15033..16157
                     /gene="recF"
                     /locus_tag="SDA06_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG4227816.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            16154..18100
                     /gene="gyrB"
                     /locus_tag="SDA06_000522"
     CDS             16154..18100
                     /gene="gyrB"
                     /locus_tag="SDA06_000522"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG4227817.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            18120..20591
                     /gene="gyrA"
                     /locus_tag="SDA06_000523"
     CDS             18120..20591
                     /gene="gyrA"
                     /locus_tag="SDA06_000523"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG4227818.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20827..21126
                     /locus_tag="SDA06_000524"
     CDS             20827..21126
                     /locus_tag="SDA06_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG4227819.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21176..21688
                     /gene="ssb"
                     /locus_tag="SDA06_000525"
     CDS             21176..21688
                     /gene="ssb"
                     /locus_tag="SDA06_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4227820.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21714..21950
                     /locus_tag="SDA06_000526"
     CDS             21714..21950
                     /locus_tag="SDA06_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG4227821.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            22093..24066
                     /locus_tag="SDA06_000527"
     CDS             22093..24066
                     /locus_tag="SDA06_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG4227822.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            24072..24524
                     /gene="rplI"
                     /locus_tag="SDA06_000528"
     CDS             24072..24524
                     /gene="rplI"
                     /locus_tag="SDA06_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG4227823.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24798..26165
                     /gene="dnaB"
                     /locus_tag="SDA06_000529"
     CDS             24798..26165
                     /gene="dnaB"
                     /locus_tag="SDA06_000529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG4227824.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26945..28237
                     /locus_tag="SDA06_000530"
     CDS             26945..28237
                     /locus_tag="SDA06_000530"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG4227825.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8645 a         5554 c         5641 g         8537 t
ORIGIN      
        1 aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc aaacttttga
       61 acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt
      121 tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga aactggcttt
      181 tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt cccttactat
      241 tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc tagcaaaata
      301 gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa tcccgtattt
      361 tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa ctactactgg
      421 atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca gtacgaccca
      481 aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt tcttgcttcg
      541 atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa agacccagcc
      601 accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg ttccacgtga
      661 aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca acgtattatc
      721 taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat tgatgattgc
      781 ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc ctttttgttc
      841 tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt cagacaaatt
      901 cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt tggttaattt
      961 tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt ccattatcta
     1021 atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt ctaccatatt
     1081 ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac gtcgccgtct
     1141 tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc tgcatgcatg
     1201 tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa tccgcgttcg
     1261 aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt gaatgtccaa
     1321 gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa atcataagca
     1381 gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa aaattccgct
     1441 ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac cacgatcact
     1501 tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt cgcgtctgca
     1561 tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt cgtcaataaa
     1621 aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg ggcagatttt
     1681 ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac tgcatcttta
     1741 tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga ttctaagtct
     1801 gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac acgtccttct
     1861 acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc gtctacttgg
     1921 cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc ttttacgatt
     1981 ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg tacaagttct
     2041 gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg gtcgatcgtc
     2101 gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa caaagttgat
     2161 tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt attcactctt
     2221 tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga cgggtcatca
     2281 tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt cgaatgactt
     2341 cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg tgcaagtcgt
     2401 acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca agatatcaag
     2461 aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt tcccattttt
     2521 ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc tttctcggat
     2581 ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc catcattttg
     2641 atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag ttactgcacg
     2701 attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac gggcttgtcc
     2761 catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag aaagcaagcg
     2821 caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt ctgcttgtgt
     2881 tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg gattcagatc
     2941 ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt caaagtctct
     3001 aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac gtcgctcccc
     3061 tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt gaaagacacc
     3121 tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg ttttacgggg
     3181 ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat catcttttac
     3241 atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc ggcctaatcc
     3301 tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact tcagctcctc
     3361 gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct gataaacgaa
     3421 tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt acttcttcta
     3481 cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact ccctcgatct
     3541 ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat tggcttaatc
     3601 cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt gctgtaaatg
     3661 aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa tcatactgac
     3721 tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt gacgtcaatt
     3781 cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt tcgtgttcag
     3841 tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag atgtctcttg
     3901 tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca tccatatcca
     3961 ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc gtcgtcgttg
     4021 ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc tcttctcctc
     4081 ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct ttggggttgg
     4141 tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca aggtaaccgc
     4201 ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt ctgtcaattc
     4261 ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag ccaagggcaa
     4321 acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa ggtcaaatga
     4381 tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata aggaagcatc
     4441 gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca gagaatcaac
     4501 gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa accctgcccc
     4561 cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg ctaatgtaag
     4621 tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg taaggttcat
     4681 cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca tttgatgatc
     4741 ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta tcataaatat
     4801 tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta acgcaaaatc
     4861 tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag aaaagccgat
     4921 agactttttc tttctagtat gactcataaa atatacacag aagagagaac tagctttgag
     4981 caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg ctggttcact
     5041 gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt ctgcagggtt
     5101 tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct gcaatttttg
     5161 ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg gaatcttttt
     5221 cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt agccttcata
     5281 tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat tttctggaat
     5341 aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc ttgctagtac
     5401 accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt cttctgttgg
     5461 tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt ttttcttcaa
     5521 ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt ctgttaatcg
     5581 caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag tcagcaaacg
     5641 ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga tgtaaccgtc
     5701 acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat tgataccggc
     5761 aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt gtccagctgt
     5821 ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg gctctactac
     5881 gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc cagcaataga
     5941 atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt gaacatagac
     6001 ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt tgtctgcaaa
     6061 acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt caacgatccc
     6121 tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg tctcacctgt
     6181 ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg tgctgaagct
     6241 aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt cgatcgtact
     6301 tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc caagttcttt
     6361 tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg aatatttcaa
     6421 ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa ctgctttggc
     6481 ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt cgacgatcag
     6541 ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg tatgtttcat
     6601 ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag ctgggccttt
     6661 tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac ccatttctcc
     6721 gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta cagaaggatt
     6781 acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt ttgatcccat
     6841 acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa cgatcacgtc
     6901 atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt cctaaacaaa
     6961 attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca acttctccta
     7021 gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt cctgaatcaa
     7081 tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg atatggcggg
     7141 tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat aaatcagaaa
     7201 tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa aaaactggtt
     7261 cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa tctgttttat
     7321 ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca tcttctgctg
     7381 tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct aatgcttttc
     7441 ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt ccagctgtat
     7501 cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca tctcgagtcg
     7561 ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt aacaaacttg
     7621 atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc agaaccttcc
     7681 cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc gctttttcaa
     7741 gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca atatttactt
     7801 ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa gcagacagat
     7861 tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc cgaatcaaat
     7921 ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct cgttttgtga
     7981 attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa atttgattga
     8041 ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta aatgtctttg
     8101 gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg ggatcaacga
     8161 tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt ccagattgat
     8221 aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata ctgattgcac
     8281 cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta atttcagcca
     8341 ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac cctttatgca
     8401 caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct ggtcatagat
     8461 tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt gagcctgttc
     8521 tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct ttggtttatt
     8581 cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg gtacactaaa
     8641 tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc ccgttcagat
     8701 ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta tctacaggga
     8761 atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc ttagaagctg
     8821 atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag aggaaattaa
     8881 agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt gagtatcagc
     8941 aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca gtgatccaat
     9001 ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa aactgaagac
     9061 agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata ctctgataag
     9121 aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat ttattcaaaa
     9181 agtaatggaa tcaataatat tttttagcag gttctactac taaataacga taaggttcct
     9241 caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg atttgtttac
     9301 gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt actttttcag
     9361 ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa ttgcctacat
     9421 tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt gccagatatt
     9481 gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt tgtgtttcta
     9541 aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct ggagcatcaa
     9601 gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct tcatcaccta
     9661 gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc tcgatctcag
     9721 ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca gaaacttttt
     9781 cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct aggaaacctt
     9841 tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc aaatcagcca
     9901 gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt agcattacct
     9961 cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac gttctctttc
    10021 gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt tgttgatcag
    10081 cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa gaccgctggc
    10141 taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga tcttcattga
    10201 tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag taaagattgc
    10261 tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc ataagaaatg
    10321 cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca agattggcat
    10381 ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg catataaacg
    10441 ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat attgttgctg
    10501 cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg tttggaaatg
    10561 catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc ctatccctgc
    10621 attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat aacgatccca
    10681 aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga cgatgaccaa
    10741 actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact cttttcctct
    10801 ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac ctcatcataa
    10861 gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg atcaatttct
    10921 tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg gtttaccgcg
    10981 ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc cggtttttcc
    11041 aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa cacggcttga
    11101 aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc cttttctatc
    11161 tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat ttccctaaaa
    11221 aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac ttttcttcct
    11281 ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg tttacggaat
    11341 ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat atattccacc
    11401 tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat aacgacaaag
    11461 tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca caagattatt
    11521 tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca cagataataa
    11581 aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat caggcgattt
    11641 cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac agacgcaaga
    11701 ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg aaaacttttt
    11761 ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat tgtaattaaa
    11821 aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat tagtactagt
    11881 tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct ttatggaatg
    11941 aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca tggatcgaaa
    12001 cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct agcaagattc
    12061 ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt tatatgttat
    12121 caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa gaagaagtta
    12181 tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc
    12241 ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct catgcagcag
    12301 cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt tatggaggcg
    12361 tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg caaagtcagc
    12421 ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc atcaattcga
    12481 tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat ttactgctag
    12541 tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt ttccatactt
    12601 tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg
    12661 agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg tctgttgata
    12721 ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg gcggctgaac
    12781 gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat tctaacattc
    12841 gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat agtgaagata
    12901 tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa ggacatcctc
    12961 aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc caattgaaag
    13021 atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt
    13081 tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc ggtggcaagg
    13141 atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat tctagtgtca
    13201 gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt gtcgattcta
    13261 tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata cacacttttt
    13321 ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga atcaacatgc
    13381 cctattactt ttattattct ttttaactaa taataaataa acaaacattc aaaggagata
    13441 catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa ctgttcaacg
    13501 agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca cactgacaca
    13561 agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt ttttgtctgt
    13621 tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt tacaagcacg
    13681 tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag aagttttaga
    13741 aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg gattagatgc
    13801 agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat tacctgtaca
    13861 cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc
    13921 aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg tagctactga
    13981 ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc attttgatat
    14041 tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg aagaagaaat
    14101 cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga tgtatttcta
    14161 ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt caagttttaa
    14221 tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg cttctttact
    14281 ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg cggttgtttt
    14341 atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata cagcaagcag
    14401 tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt
    14461 tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta cattggaacc
    14521 aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa attaacaatg
    14581 attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa ttttaaacag
    14641 aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca ccttttttag
    14701 attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg aaaaaaaagg
    14761 tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac aacaagtcat
    14821 tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag ttatcggtag
    14881 tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg gcgaaccaga
    14941 aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag aagtaggaac
    15001 tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg tatttaaaaa
    15061 attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc atctttttag
    15121 gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta gctatgacgc
    15181 gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat caggcgatga
    15241 ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta ttatctaaaa
    15301 aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact tacgtgggac
    15361 aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc agcccgcaga
    15421 ccaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat ctttatgatc
    15481 ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa
    15541 aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca gcgtttggca
    15601 gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg gctaattctt
    15661 tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc accgcagtcg
    15721 attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca cttttgaacc
    15781 aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat cgtgacgatc
    15841 tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa
    15901 caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag acaggggaat
    15961 atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt caactccatc
    16021 tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca cttgaccatg
    16081 taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg aaactaaaag
    16141 gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag agtatgatgc
    16201 cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg gcatgtatat
    16261 cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg ataattcgat
    16321 cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa aagacaacag
    16381 tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa aaactggacg
    16441 tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg gcggtggtgg
    16501 gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgttaacg cgctttctac
    16561 acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg
    16621 agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa caacggttca
    16681 ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt caaaattggc
    16741 tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa
    16801 acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatta aaagttatgt
    16861 cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt tggaaggtga
    16921 acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc attccaatat
    16981 catgagtttt gccaacaata tccatacgta tgaaggcgga acacatgaat caggattcaa
    17041 gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa
    17101 tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta tttctatcaa
    17161 gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg
    17221 gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag
    17281 tgtgggccgt caaattgttg aaaaaggatt actagcttcc agagctcgtc tagcagcgaa
    17341 acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc tgccaggaaa
    17401 attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga
    17461 ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca tcttgcctat
    17521 tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg caaatgaaga
    17581 gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg atgtatcgaa
    17641 agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg cgcatatccg
    17701 tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag ctggatacgt
    17761 ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta cgtatgtaca
    17821 accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc
    17881 aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc aactatggga
    17941 aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg atgctattgc
    18001 agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc gtgcattcat
    18061 cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga ggaaaaagaa
    18121 tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa atgaaagact
    18181 ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat gtcagagatg
    18241 gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt gtaacaccgg
    18301 ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa taccatcctc
    18361 atggagacta tgcgatctat gagtcaatgg tacggatggc acagccattc agctaccgtt
    18421 atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt gccgccgcta
    18481 tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt gatatcaata
    18541 agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca gttgtactac
    18601 ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta ggtatggcca
    18661 cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg ctgatggata
    18721 atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat tttccaacag
    18781 gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt aagggttcta
    18841 ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa cggatcatcg
    18901 tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt tctgaactgc
    18961 acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc cgtgaaggta
    19021 tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg aataacttat
    19081 ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc gaaaaaggtg
    19141 tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag
    19201 tcatcacaag acggacagtc tttgaaaaag aaaaagcaga agcaagggca cacattttag
    19261 aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc cgcgggtcta
    19321 aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc gatcgccagt
    19381 ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg
    19441 aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt ctggcaagac
    19501 cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa cgatttggtg
    19561 ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat gaagatttaa
    19621 tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa cgtttggcaa
    19681 ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca
    19741 atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg ttattcttta
    19801 cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac gggcgcacag
    19861 ccaaaggaat accaattatc aatttactag gtatcgattc gagtgaaagc atccaggcaa
    19921 tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg acgcgtcaag
    19981 gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg
    20041 caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat ggtcaatcta
    20101 atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa gcagtccgcg
    20161 atatgggacg tacagcttcc ggggttagag gaatacgcct gcgtgaggaa gattatgtcg
    20221 ttggggcagc cttaatgaag gaagatcaag aagtattagt gatcacagaa aaaggttacg
    20281 gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa
    20341 cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt tctggagatg
    20401 aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta tcgactgttt
    20461 ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct gatacgaaag
    20521 ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa gaacaaggat
    20581 cagaagaata gccatcaaga ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc
    20641 tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt cgaaactgtt
    20701 cattctgtat tgcttttgac caaaatagag tgtataatca gagcttgtga gtagtctaat
    20761 taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac agggaggtga
    20821 atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa cattgatgaa
    20881 gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa cggagcagaa
    20941 gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt
    21001 gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc tgtcaatgaa
    21061 tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt taaagaagaa
    21121 gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga ctattttgat
    21181 taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt acacagcaag
    21241 tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa accaaagcgg
    21301 caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag aaactttagc
    21361 aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa ctcgttctta
    21421 tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca acttccaatt
    21481 gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg
    21541 tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc aatcatccaa
    21601 cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt caacgatcga
    21661 catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat agaatggcac
    21721 aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct aaccatattg
    21781 aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa cgcggtaaaa
    21841 tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa
    21901 tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa taaatcctag
    21961 gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg taaaaataac
    22021 ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt agtcatatat
    22081 gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct cttccttatc
    22141 ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt tgttagcgct
    22201 tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat gaaacgttgg
    22261 cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga aaccaatcta
    22321 gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc aagccaaaaa
    22381 atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt aacagaggaa
    22441 gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca
    22501 gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt taatgatatt
    22561 acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat tggtatttta
    22621 tctattgata actatgcaga tgccattgat aaattggatg acaaagagat ttcttatttg
    22681 aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt ctttttcaaa
    22741 agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa aaagatgatg
    22801 gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct
    22861 atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa
    22921 gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt cgtgaaagag
    22981 gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc
    23041 acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga tgaaacaggc
    23101 gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc tgcttttggt
    23161 gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa cgagaacgag
    23221 atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga attagaaatg
    23281 caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct cctcgtgatg
    23341 gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca atttgataag
    23401 attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc tctgctgact
    23461 tatatcgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat ccaataccaa
    23521 agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc agggatggtt
    23581 gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt tgccagctat
    23641 ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc ggatttagcg
    23701 acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc tgatattgtt
    23761 gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa aacagcagat
    23821 acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg gacagacggc
    23881 ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat catggaatcc
    23941 ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac gacagttgag
    24001 gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat atatgataag
    24061 gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt aaaaaagggg
    24121 acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat aacttggcaa
    24181 aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca caagaaaaac
    24241 acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa aaagaagata
    24301 ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt tcgatccctt
    24361 caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat aaacgaaaaa
    24421 tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt aagcttcatc
    24481 atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc ttgatagcga
    24541 tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa
    24601 cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc ggtttctttt
    24661 ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact atttttttat
    24721 tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga agcaattgaa
    24781 gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg caaaatattg
    24841 aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc attgatgcca
    24901 tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt tttgagacaa
    24961 tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg
    25021 aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac ttggcattag
    25081 cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa aaatcattgc
    25141 tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag caaggagaag
    25201 aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa
    25261 atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa aatatcgatc
    25321 gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa ggtctggata
    25381 aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt ccagcggtag
    25441 gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca gatcgttctg
    25501 ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg ctttgtgcag
    25561 aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa gaatggtcga
    25621 atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat gatacaccag
    25681 ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc
    25741 taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa gagaaccgtc
    25801 aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac
    25861 ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac aaacgaccag
    25921 tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc gtagccttct
    25981 tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga aacgagccaa
    26041 aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca cgaggaacag
    26101 ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca agagaagaat
    26161 tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg
    26221 agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg gattgtgaca
    26281 aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg acccttggta
    26341 ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
    26401 ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg ccaactcctc
    26461 ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg ctccttgtga
    26521 ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc
    26581 tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt ggctgttctt
    26641 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc aaaagtagct
    26701 tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
    26761 cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc attctaaaaa
    26821 gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt cgattttatt
    26881 tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag aacgaggtgt
    26941 tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga aaggaaaaat
    27001 cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg gcgacaatgc
    27061 agggcacaca attaagttta acggtgtgac ttataaactg catttgattc cttctgggat
    27121 tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt
    27181 ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc
    27241 tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac aagaagatgc
    27301 taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct atatggataa
    27361 agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt ttgaagaacg
    27421 tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg attcagaacc
    27481 acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa tcaaacaata
    27541 cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt
    27601 tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt ttgttacttc
    27661 ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt caaaaatcaa
    27721 taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac catttccaac
    27781 tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat acggtacgac
    27841 aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc attctaaacg
    27901 agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg gattggaaac
    27961 agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact atccagcaag
    28021 cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga
    28081 cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact atgtacgtcg
    28141 tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg atagaacaca
    28201 aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac gactgaatca
    28261 ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata acttgaacac
    28321 tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DARDHD010000014        28374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000014 DARDHD010000000
VERSION     DARDHD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.014"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            65..289
                     /locus_tag="SDA06_000531"
     CDS             65..289
                     /locus_tag="SDA06_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG4227826.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            431..2182
                     /locus_tag="SDA06_000532"
     CDS             431..2182
                     /locus_tag="SDA06_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227827.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            2182..3966
                     /locus_tag="SDA06_000533"
     CDS             2182..3966
                     /locus_tag="SDA06_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227828.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            4292..5143
                     /locus_tag="SDA06_000534"
     CDS             4292..5143
                     /locus_tag="SDA06_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4227829.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            5197..6948
                     /locus_tag="SDA06_000535"
     CDS             5197..6948
                     /locus_tag="SDA06_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4227830.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            6952..7122
                     /locus_tag="SDA06_000536"
     CDS             6952..7122
                     /locus_tag="SDA06_000536"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4227831.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(7162..8064)
                     /locus_tag="SDA06_000537"
     CDS             complement(7162..8064)
                     /locus_tag="SDA06_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4227832.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            8295..9698
                     /locus_tag="SDA06_000538"
     CDS             8295..9698
                     /locus_tag="SDA06_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4227833.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            9698..11131
                     /locus_tag="SDA06_000539"
     CDS             9698..11131
                     /locus_tag="SDA06_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4227834.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            11276..12202
                     /gene="trxB"
                     /locus_tag="SDA06_000540"
     CDS             11276..12202
                     /gene="trxB"
                     /locus_tag="SDA06_000540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG4227835.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            12416..14143
                     /locus_tag="SDA06_000541"
     CDS             12416..14143
                     /locus_tag="SDA06_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG4227836.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            14158..14508
                     /locus_tag="SDA06_000542"
     CDS             14158..14508
                     /locus_tag="SDA06_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4227837.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14600..15460)
                     /locus_tag="SDA06_000543"
     CDS             complement(14600..15460)
                     /locus_tag="SDA06_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG4227838.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            15552..15980
                     /locus_tag="SDA06_000544"
     CDS             15552..15980
                     /locus_tag="SDA06_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4227839.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            16142..16318
                     /locus_tag="SDA06_000545"
     CDS             16142..16318
                     /locus_tag="SDA06_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG4227840.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            16345..16791
                     /locus_tag="SDA06_000546"
     CDS             16345..16791
                     /locus_tag="SDA06_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG4227841.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(16945..18324)
                     /gene="brnQ"
                     /locus_tag="SDA06_000547"
     CDS             complement(16945..18324)
                     /gene="brnQ"
                     /locus_tag="SDA06_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4227842.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            19030..20001
                     /locus_tag="SDA06_000548"
     CDS             19030..20001
                     /locus_tag="SDA06_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG4227843.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            20025..22217
                     /locus_tag="SDA06_000549"
     CDS             20025..22217
                     /locus_tag="SDA06_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG4227844.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            22234..22710
                     /gene="ybeY"
                     /locus_tag="SDA06_000550"
     CDS             22234..22710
                     /gene="ybeY"
                     /locus_tag="SDA06_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG4227845.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            22694..23089
                     /locus_tag="SDA06_000551"
     CDS             22694..23089
                     /locus_tag="SDA06_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG4227846.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            23104..24003
                     /gene="era"
                     /locus_tag="SDA06_000552"
     CDS             23104..24003
                     /gene="era"
                     /locus_tag="SDA06_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG4227847.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            24146..24958
                     /gene="recO"
                     /locus_tag="SDA06_000553"
     CDS             24146..24958
                     /gene="recO"
                     /locus_tag="SDA06_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG4227848.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            25342..26259
                     /gene="glyQ"
                     /locus_tag="SDA06_000554"
     CDS             25342..26259
                     /gene="glyQ"
                     /locus_tag="SDA06_000554"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG4227849.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            26261..28336
                     /locus_tag="SDA06_000555"
     CDS             26261..28336
                     /locus_tag="SDA06_000555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG4227850.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         9342 a         4968 c         6151 g         7913 t
ORIGIN      
        1 tttcaataaa ctttagtaaa ataagtcatt gagagcaatt cggaaataga aaggagagat
       61 ttatatgtta tcaacaggta ttgtcgtact tattgctatt attgctttgc taatcggcgc
      121 agtaggtggc tttttcctag cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc
      181 cgttaatgag gacatgttgc gatcaatgat gatgtcaatg gggcaaaaac catctgagaa
      241 gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca
      301 acgacttttt acgggctgat tttttcagtc cgttttttat ttcgtaattt gttagactat
      361 tcagattatt cagcaaaaaa gacaagcaca gacaatccta ggctcttgat aaagacaaag
      421 gaggagtaaa atgtcgattt ttagaaaatt aggctggttt tttcgtcagg aaaagaaaca
      481 ttatttaatt ggggttttct cgctattcgc agtggccttg gtacagttag ttcctccaaa
      541 agtgattggg atcatcattg atgagatcgc cgataaaaat ttatcaataa agatcatttt
      601 cgggtggatc ttcctattga ttatcgctgc ttttttacaa tatctttttc gttatatctg
      661 gcggacgaat atttggggaa gtgcagctcg attggagaaa gaacttcggc agcagttatt
      721 caaacatttt acagaaatgg acaatctctt ttaccaaaaa tatcggactg gggatttgat
      781 ggctcacgca acgaatgatt tgaatgcgat ccagaatgta gcaggagctg gtatcttgac
      841 ctttgcagat tcagtgatta cgggcggaac aacgatcatt gcgatgattt tgtttattga
      901 ttggcgctta acactgattg cgctgattcc tctgcctttg ctggccgtca cttcacgagt
      961 gctggggtca aaactccatg atgcttttcg tgattcgcaa gaagcttttt ctagtatcaa
     1021 tgataagaca caagaaagta tcactgggat caaagtcatt aaaacatttg gacaagaaaa
     1081 agaagatcta gcggattttg aagaaaagat tgaagatgca attattaaaa acaaacgagt
     1141 caatttttta gactccttat ttgatccatt cattaccttg attatcggtc tgtcttatgt
     1201 tgcaacaatt attttaggtg gaaagtatgt cttggaaggc acgatctcga tcgggcagat
     1261 ggtctcattt attagttata tcggtatgct tgtttggccg atgtttgcga ttggtcgatt
     1321 gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc gtcaatgaac ttttgcatga
     1381 aaagacgcat atcgtggaac aactgggtgc agtgcagacc ccggcaaaag gcgagctgaa
     1441 tgtagctatt tcctctttta attatccgaa agaagaatca ccagcattag aacaaatcaa
     1501 gtttgcgatc caagaaggag aaacattagg tattgttggg aaaactggtg cgggaaaaac
     1561 gacgatcatc aaattactga tgcgtgaata cgatgattat caagggaaaa tccaatttgg
     1621 cggacgttcg atcaaagaat attcattgaa tgctttatta ggatcaatcg gctatgtgcc
     1681 tcaggatcac ttcttgtttt cgatgaccat tcgagataat atccgttttt ctaatccaaa
     1741 actttcagaa gaagaagtag agcatgcggc ttctttagca tttatcaaca atgaaatcaa
     1801 ggaattacca aaaggttatg ataccttagt tggagaacgt ggagtctctt tatctggcgg
     1861 acagaaacaa aggatttcga ttgctcgagc attgatcaaa aatcctgaat tattaatatt
     1921 agatgatgca ctctctgcag tagatgccaa aacagaagaa gctatcttgc ataatttaaa
     1981 agaaataaga aaagagaaaa caactatcat tgcagctcac cgtttaagta gcgtgatgca
     2041 tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt gaacgaggaa cccatcatga
     2101 gctgcttgca ctaggcggct ggtatgaaca aatgtgggag aaacagcaat tagaagcgaa
     2161 gatagaagga ggcgaggcct gatggatgaa aattatcaat cggaatggac aaaatcgatt
     2221 ccacttaaag aacagaaaaa gatcatcaaa cgactgatga agttcgccaa accttttaga
     2281 agaacgttca tcgttgctat cttatttgcg tttgctttgt ctgtgatcaa tgtcctattg
     2341 ccaaggatca tccaaacttt tatggatgat catctggcta aacagtcggc tacgacacaa
     2401 gttattttgt tttttgctgg tatgtactta tttggagtca tagtaaaaag tatcatttgg
     2461 tttttccaat ggtatttgta ttccatggcc tcattgaaga cctatcagta tattagggta
     2521 aaattatttg aaaagctgca tacactaggt atgcgttatt ttgatcagac accggcagga
     2581 tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat ttgaattttg gtacgtcttt
     2641 ttgatggtat tgactgggat ctttgcagtc gtttcttcat tccttgccat gtttcaaatc
     2701 aatggagaaa ttgccttgtg gactttggtg tttttgccga ttttaggaat cgtcatctgg
     2761 tattaccaga aattcagttc tcgtatttac cgaaatatgc gggaaaaatt aagtcagctg
     2821 aacacgaaat tgaatgaatc gatttccggc atgcgtatta tccagcagtt tcgccaagaa
     2881 gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt atttagacac gcgttatgcg
     2941 atgatcaaaa cgaattcgat tctgttaagt ccgatcatca attttctata tgcattggcg
     3001 attgcgttgg tgttgacttt gtttgggatc gatgcgttgc attctcccgt agaagttgga
     3061 ctgatctatg catttacgac ctatgtccag ggatttttta atccaatgtc tcaaatgatg
     3121 gactttttga gcatcttcac agatgggatg gttgctggta gtcgtatctt gaagattatg
     3181 gatacggaag aatatgcacc acaacaaaac cctggagcag atcaaatgat tacttcaggt
     3241 aagattgagt ttcgcaatgt aagcttttct tatgatggag agcacaatgt attaaacaat
     3301 atctcttttg ttgctaatcc gggagagacc gttgcattag tgggacacac cggaagcgga
     3361 aaaagctcga tcatcaacgt attgatgcgg ttttacgaat tttatgaagg tcagatattg
     3421 attgatgatc atgacattcg agaatatcca atggaagagt tacgtaagaa aatggggctg
     3481 gtcttgcaag atgctttctt gttttatggg gatatcgcag gaaatattcg gatgatgaat
     3541 ccagctatca cagacgaaca gatacgccat gcggctgagt ttgttcaagc aaacaaattc
     3601 atcgatgaat taccaaaagg atatcactca aaagtgatcg aacatggtgc cagctattct
     3661 agtggacaac gacagctgat cacttttgcg cgaacaatcg tgactgatcc taaaattcta
     3721 gtattggatg aagcaacagc aaatatcgat actgaaacag aaggattgat ccaagaagga
     3781 ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg cccatcggct gtcaacgatt
     3841 cgagatgcag aattgatttt agttttagac aaaggtcata tagtagaacg cgggaatcat
     3901 gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt acaaactgca aagcagtggt
     3961 atataaaaga tgttgtgaca gtagaatttt gacctaagca ctagagtgaa aataaaacat
     4021 agcttctcat atttttcatc tgtgagagtt agtgtcgtag tcaccagatt taaaaaaaga
     4081 ttcgacagct agtcagaagt gcctaatagc cactaagaaa gcgttgacaa aagcatttgc
     4141 tttcgataat ataaagaaaa cgatacaact tggatagtga ttgcctgatg caagctaaac
     4201 ctgatgagtc tcccatggtt tcatggacgg ctttttgggt ttttcttttt gttttggata
     4261 cagccctata catcatggaa aggagcaaaa catgctgatt gagaaagtac tgaataacaa
     4321 cgtcgtaata accagggatg aaagaaatca ggaaatgatc gtgatgggaa aaggtttggc
     4381 attcaaaaag aaagtgggtg acccgatttt tgactcatgc atcgacaaaa aatttcagtt
     4441 gacaaatgac gggctttcac aacagttcca agaattggca caagcgattc ctcttgagta
     4501 cttagaaatt tcttgtgaaa tcattgaata tacaaaagag gctctacaag tccagttgaa
     4561 tgattctgtg tatattgcat tgacggatca tttgtatacg gctatcgaac gtcacaaaat
     4621 gggaatattt gtaccaaatg tattgctgta tgatatcaaa caactatttc ctaaagagta
     4681 tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa taccacatca agttatcaga
     4741 agatgaagcc ggatttatcg cattgcattt agtcaatgca caaggcattc aaagccagca
     4801 agacaatacc tacctgttta ctataacaat acaggatatt ctaaatatcg tccgctatta
     4861 ctataagatt acatttgatg aagattcagt ccattttttt cgattcgtca cacacttacg
     4921 tttttttctg acacgcgtcc tagatggaga aaaatcagaa gtgggagaag tttctgagga
     4981 attgtttcag gttattcaaa aaaaatataa ggatacgttt ggttgcgttg agaaaatagc
     5041 agacttttta gccgagacgt tcaactacca gatgtccacg gacgaaaaaa tgtatttaac
     5101 gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa taaaaaaagg aggataaatt
     5161 aacaagaacg tatgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     5221 ttggagggaa tagaaatggg aaaataccaa gaattagcga gaaaaatcgt agaaaatgtc
     5281 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     5341 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtat tgtaacggta
     5401 atgcgagcgg gagggcagta tcaagttgtc attgggaatc atgtgccagt cgtgtttgaa
     5461 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     5521 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctatccta
     5581 gctgctggcg ggatgattcg tggactgaca gcattccttg tcttcttagg cgcgtttgac
     5641 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     5701 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     5761 ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     5821 gaagcaccac taacgaccat attttctggc actatttttg aagcacctat ttatcaaaca
     5881 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     5941 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     6001 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcctttagg ttttttactc
     6061 gttggaccag tattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     6121 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctggtgatg
     6181 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     6241 caaaatatta tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttaccgca
     6301 ctctacttta agcttagaaa tccaaaagac aaagcaattg caataccagc cattgtttct
     6361 ggtattgtgg ggattacaga gccagctatt tatgggttta cattaccacg taaaaaaata
     6421 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtgggtt aatgaattta
     6481 acttcttgga atcaaggtgg actaggcatc tttacaattc ccaactatat tagaccagat
     6541 ggtgatttaa cagatgtgat taatgtattg attgggatcg ccattgcgat ggtggtatcc
     6601 tttacgctga cttttttctt ttggaaggat gaagcaggtg aaacagatga tatacagaaa
     6661 aaatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actaaatgca
     6721 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     6781 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacag
     6841 gcaattggaa tggtttctga aacagggctg gaattgctga ttcatgtcgg attggatact
     6901 gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc aaggataaag agtgaaacaa
     6961 ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa
     7021 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa ccaaggggag
     7081 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaagaatg
     7141 gactaggctt aactattctt tttattccac ttctttattt tcaaaattga aaggctcttt
     7201 catgatggct gagctgatag ggcgtttatc gaatttttga ccgacagaaa ttagatggac
     7261 taaattctgt tgataatttt ctgcaaaaac acaactatat aacacgtcta gaagataaca
     7321 aattgatgta ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag
     7381 tcgcaaagcg gctgtactca ttgcagatag cgttgaatca ccgatactgg tcaaagctaa
     7441 aattggaaca tgattttgtt tcaatatttc tgcggtacga aggatcacat tgttttcacc
     7501 cgtataagaa atcagaatcc cgcacatatc actcgtgcaa ttgtacgctt catagaatga
     7561 ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg cgcttcattt tcagcatgaa
     7621 atcttgagaa atcaaagtat tcgagttact ggcgaaaatc acgatttttt ttgcttgaag
     7681 cattagttgt ttggcttttt gcagttggtc atgatgaatg agagaaagtg tatcatgaat
     7741 cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc ataataccgt ctgtggtgga
     7801 aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca agataggctg tttcagctaa
     7861 aaaagcatta cgaaaactga cccagccatc aaaacccatt ttttttgcta cacggatgag
     7921 tgtagagggg tggacataac atcgctcagc aatcgcttgg atggtcattt cgccaagtgc
     7981 tggagaatcg cttaagagaa actggatcac ttcattttct gaaggagaaa aggagatttc
     8041 tttgattttt tcacgtaata acatgtagat accttcttct tattttttta cgtaaattat
     8101 aacttttttg aaaatgattt gtaaacggat ttatactata taacttaaaa tagacatttt
     8161 ttgacaagat atttacaaaa atccactaga aaacagtaaa ttttgtaaat gttactggaa
     8221 agaaacagat ttctattgtt tgttttgttt acaaaaaaga taatgaaagt gtaaaaaagt
     8281 aaaggagtga gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt
     8341 acacccccga gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa
     8401 tctggctcgt agatgtagaa gaaggaaaag aaaagcttga gatcgttagt cagatgtctc
     8461 agcgtatgtg ggatgcttct ccgtatgaag tcaagatcta tgcaactctg gatcgggaag
     8521 aagcattgaa agatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac
     8581 gaatcaaaga cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg
     8641 ctggcgggat attcaaagct tttcgtaccg ttccagtcgt tttggcaatc gttgaggaca
     8701 tgaagcgttt atgtccaaat gcttggctga tcaatttcgc gaacccaagt gggatggtga
     8761 cagaagcagt tatcagatat ggcaaatggg ataaagtgat tggattatgc aatgtgccag
     8821 tgatggcgat gatgacagaa ccagcaatgc ttggcaagaa aaaagaagag ctgatctaca
     8881 agtttgccgg tctaaatcat ttccattggc acaaagtagc agataataaa ggaaatgacc
     8941 gcacggcaga attgattgat cgtttatacg atgaagagaa tggattaccg aaaaatatct
     9001 ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa catgatccct tgtggctacc
     9061 accgctatta ctatcgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga
     9121 ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga gctatttgaa ttatacaaag
     9181 atccagaatt agaccataaa cctgaacaat tgcaaaaacg aggtggcgct tattattcag
     9241 acgctgcctg tgaaacgatt gcttcaatct atggaaataa agaaacacag atcgtggtat
     9301 ctacaaagaa tgatggagct gttcctgacc tgccggcaga ttgtgtagta gaagtcactg
     9361 cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg ttctcttcct gctgctgaaa
     9421 gaggctggtt gcaagtaatg aagaatatgg aattgctgac gatcgaagca gctgtgactg
     9481 gtgattatgg gacagcttta caggcgttta cgatcaatcc acttatacga tcaggaaaga
     9541 cagcaaaacg gatcatggat gagttgttta ttgcacacca gcagtattta ccgcaattca
     9601 aggagacaat cagccgatta aaagaagaag gaatcgaagt tcaagatgaa ctggcaagag
     9661 aattatcagg taatacacat ataatggagg aagtataatg ggattcagaa aagatttttt
     9721 atggggcggt gctacagcag ccaatcaatg tgagggcggt tatgatgaag gtggacgcgg
     9781 actagcaaac gtagatgttg tgccaatcgg aaaagaccgt tttcctgtta tcaccggaaa
     9841 gaaaaaaatg tttgaatttg atgaggatca tttttatcca gcaaaaattg caatcgatat
     9901 gtatcatcgt tataaagaag atattgcttt gtttgcagaa atggggttca agacttatcg
     9961 aatgtccatc gcctggagtc gtatttttcc aatgggggat gagaaagagc cgaatgaagc
    10021 tggcttgaaa ttttatgagg atgcctttaa agaatgccaa aagcacggaa ttgagccact
    10081 tgtgacgatt acgcattttg attgtccaat gcatttgatc aaagaatatg gtgcgtggcg
    10141 ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga gtcatcttca atcgctataa
    10201 aggacttgtc aaatattggc tgacattcaa tgagatcaat atgatccttc atgcaccatt
    10261 catgggcgca gggctgtatt ttgaaaaagg agaaaatgaa gaacaaatca aatatcaagc
    10321 ggcacatcat gagttggttg caagtgccat cgcaacgaag attgctcatg aagtagatcc
    10381 ggagaacaaa gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc
    10441 tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca
    10501 gtcacgagga gaatatcccg cttatgcatt gaaagaaatg gagcgaaatg gcattcagat
    10561 cgaaatggaa ccaggggatg ccgcattgct gaaagaacat actgttgatt ttatctcttt
    10621 ttcttattat gcttcccgag tttcgactac cgacaacgaa ctcttggaac agactgcagg
    10681 aaatatcttt gcttctgtca aaaatccata tctgcaagca agtgaatggg ggtggcaaat
    10741 tgatccatta ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccatt
    10801 gtttattgta gaaaacggct taggtgcagt cgatacgcca gatgaaaatg gttatgtgga
    10861 agatgattac cgaatcgatt acctggcagc gcatatccag gcgatgaagg atgcagtaga
    10921 actagacgga gtcgatctat taggctatac gacttgggga tgtattgatt tagtatcagc
    10981 tggtaccggt gagatgaaga aaaggtacgg attcatttat gtcgatcgtg ataaccaagg
    11041 aaatggcaca ttagcccgtt caaagaaaaa atcattcaac tggtataaaa aagtaattga
    11101 ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt tataaaagtc tattgttttt
    11161 cagcggattt ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt
    11221 ttttcgcatt ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta
    11281 tgatgtaatc gttattggcg caggtcctgc tgggatgact gctgctttat atgcttcacg
    11341 ttcaaactta tccgtattga tgatcgaaca aggtgcccct ggcggccaaa tgaacaatac
    11401 ggcagaaatc gaaaattatc ctggattcga ttcgatcatg ggccccgatc tagcttataa
    11461 gatgtatgaa agtgtctcaa gatttgggac agaaaatgct tatggaatcg ttcaagatat
    11521 caaggatcac ggcagccata aagaagtcat ctgtgaagat aaaagttacg aaggaaaaac
    11581 agtcattatc gcaacaggat gtatccaccg taaattaggc gtacctggcg aggaagaatt
    11641 tgctggacga ggcgtttctt actgtgcagt ctgtgatggt gcgtttttcc gtaacaaacg
    11701 tctagtagtc atcgggggtg gggactcagc agtagaagaa gccatctatt tgacacaatt
    11761 tgcttctgaa gtagtgatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca
    11821 agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat
    11881 cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga
    11941 atttgattta gcagcagacg gagtattcat ctatgttgga ctggatccat taactgcacc
    12001 ttttagagaa gcaggtttga cgaacgaagc tggctggatt ccaacagaca acgaaatgaa
    12061 aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat
    12121 cactacagct gttggtgacg gcggtattgc tggacaacaa gtgtataaat acttggagtc
    12181 tttagaagaa acaacagcat aagtttaaaa agagtagaag aacgggttca attgttcttt
    12241 tactcttttt tttcgcgtga aatttactga atctgattta tgcgaacacg tccaacacct
    12301 tttgataaaa gaatcctttc ttgatttaag cgttttttgg tcctcatact ttcatatcta
    12361 ataagtgaat gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc
    12421 ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa
    12481 acaattaaat gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt
    12541 ggaattcggt actgcaggta tgcggggaat cttaggacct gggatcaatc gaatgaacat
    12601 ctatacggta agacaagcaa cagaaggact agcacgtttc atggatacac aaggtgaaga
    12661 aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc catcagtcac cagaatttgc
    12721 aatggaagca gccaaaacac tagcacatca caatatcccg tcttatgtat tcgaaagtct
    12781 gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg aagacgtttg cgggaatcat
    12841 ggtaaccgca tctcacaacc cagcggctta caatggatat aaagtctacg gagaagatgg
    12901 cggacaaatg cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa
    12961 tccgctaaaa gtcgaagtat tatctgacga agaagctaaa catagcgggt tgatcacgat
    13021 catcggagaa gaagtggacg ctgcttatct agaagaaatc aaagcagtga cgattgatcg
    13081 tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg tacacgccac ttcatggaac
    13141 agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt
    13201 tcccgaacaa gctgtggcag atccagattt cactacggtc aaatcgccaa atccagaaga
    13261 acattcagct tttgaatatg ctatacgact tggagaaaaa gaaggagctg acttgctgat
    13321 tgcaacagat cctgatgcag accgtttagg cgcagcggtt cgtttgccag atggttctta
    13381 tgaagtattg actgggaacc aaatcggagc attgtttgct caatatattt tggaagcaca
    13441 tcaatcaaga ggcactttac cagaaaacgc agcggtattg aaatcaatcg tttccagcga
    13501 attgcctaca gcaattgcgg aacactacgg tgctaccatg ttcaatgtgt tgacagggtt
    13561 taaattcatt gctgaaaaaa tccaacaatt tgaagaagac cattcacata cttttatgtt
    13621 tggattcgaa gaaagttatg gttatttagt taaacctttt gttcgcgata aagacgcgat
    13681 ccaagcattg cttttattag cagaagtagc agcttactat aaaaaacaag gcaaaacgtt
    13741 gtatgacggc ttgcaaacga tttttgaaac ctatggctat ttcgcagaaa aaacgatttc
    13801 tgtgacaatg agcggacaag aaggttctgc gaaaatctct gctttgatgg aaaaattccg
    13861 taaagaagca ccgacagaat ttgccggagc aaaagttatt caaacagaag actttaaaga
    13921 attgacgaga acagaccgtg aaggaaacgt agaaaaacta acaacaccgc catccgacgt
    13981 attgaagtat acattggaag aaaatggctg gatcgctatc cgcccttcag gaactgagcc
    14041 gaaaatcaag ttttatattg gtgtaaaagg aagctctaca gaagacgcag ccgaaaaagt
    14101 agcctcttat gaagcgatta tcaaaaagat gacagaagaa taaaaaactt gagactgatg
    14161 gaaaaagtgg taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca
    14221 gacacgcaaa aatcaaaagt agagaaagtc agtcaattat tcaaaatgtt gagtgatccc
    14281 actcgtctta aaattctgct atatctgaaa gacggggaac aaaatgtgac agcaatcacc
    14341 caagcagtgg aaatggagca gtctgctgta tcgcatcaac tacgtttatt aagagaaaat
    14401 catgtcgtca agtctcatcg cgagggaaaa gcgattttgt atagtcttga tgattctcat
    14461 gtactggata ttttgaacca gacactcaaa catgtggaac atcaataacg ataggtgaaa
    14521 atagataaac actggagtgt gacaacgaag aaatttcgtt gtcacactcc agtgtttttt
    14581 aagaaaaaga caaaaatgat taatcgcttt tttctgttgt ttcagttcca taataactat
    14641 gatcttctag gtctaaagca ttcaaaataa gcgaagccgt ttcttcaata gataaagatg
    14701 ccacattgat cactacacat cctagttttt cataaagatc attcgcaaaa tcgagttcag
    14761 ctctaatttt ttcgatatcg gaataagcag tatcagggtt taatccgtaa gagcgcatcc
    14821 gttctttacg gatattattt aaaatatctg ggttattcgt tagtcccacg atttttttag
    14881 gatcgatttc ccacagctgt ttgggaatat gtgcttgggg aatcaaaggt aaattagcaa
    14941 cttttaaatt tttatttgct aaaaacaagc tcaaaggcgt tttggaagtt cgggatacgc
    15001 ctagcaaaac gacatctgct tctaaaaaac cacgaggatc tttcccatca tcgtatttta
    15061 cagcaaattc cattgctttg atccgtttga aatagttctc attcaataaa tggagggcac
    15121 caggttgccg aattggcttg ataccagtta aacgttcaat ttctgatatc ggtggcgtca
    15181 tcacatcgaa actaaataat tgatgttctt cacaaaaaga acgtgttatc tcgatcagtt
    15241 cttgattgat taatgtatgc aggacaagtg ctttttcttc taatgcatct tgcaatgcct
    15301 gccttaatgt tttttcatct gtcacgaacg ttctgcggaa taggttgaac tcaacggtag
    15361 gatactgagc catagtagct tgcgctagtt ttgtcgcagt ttctccagca gagtcggaga
    15421 tgacaaaaaa agtaacgatt tctgtagaat cggaagacat ggataaaacc tcacttgcat
    15481 tttttttatg tttattatac catactctag aatgcgtact tcgtaatcat tttgatttgg
    15541 aggaataagt catgaaaaaa cagacattga tcgaacaatc attagaaaca ttgaaaaata
    15601 acggttttaa atatactaaa aaaagagaag cattgttaac ttatttagtg aagcgtaacc
    15661 gctacgtatc tgctaaagaa gtttttgact atatgaatca ggaattcaaa ggggtaagtt
    15721 atgatacgat ttaccggaat ctccatgatt ttgcagagat cgatttactg gaagaaaccg
    15781 atttaaatgg tgaaatgaag tttcgttttc attgctgtca gggggaaatc ggccatcacc
    15841 atcattttat ctgtacagtg tgcggaacaa cgaaagaaat ccatatgtgt ccaatgaatt
    15901 atttcgaaga acagctaggt ggatgcacga tcgaagggca tagattcgag attttcggaa
    15961 gatgtgaaaa ttgtcaataa ggatttaaat aaaataacat ttgataattt aaaaaaaaag
    16021 caagatttcc ttgacggtca aaacagcatt agatataata tttacaggac agtcttgttt
    16081 tttgagatat atttcaataa caattacggt tactttttaa gctcggaggg agggaattaa
    16141 catgtcaaaa acagtcgttc gcaaaaacga atcacttgac gatgctcttc gtcgcttcaa
    16201 acgttccgtg tcaaaagcgg gtactttaca agaatctcgt aaacgtgaat tctacgaaaa
    16261 accaagtgta agacgcaaga aaaaatctga agcagctaga aaacgcaaaa aattctagtt
    16321 ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta gattaaacga tgacatgaaa
    16381 gcagcgatga aagcgaaaga taaggaatct ttacaagtca tccgaatgat caagtcatct
    16441 atacaaaacg agcagattaa agaaggtcac gacttgacag aagaagaaga actaacggtg
    16501 ttgtctcgcg aaatgaaaca gcgacgtgat tctttgcatg agtttgaaga agcaggacgt
    16561 gacgatttag ctgaaaaagt aaaaagtgaa attgtaatag tcgaaaaata tatgccggaa
    16621 caactttccg acgaagaaat tcgacaactt gttcaagaag caatcactca aactggtgct
    16681 tcatcaatga aagaatttgg caaagttatg ggtgcaatta tgcctaaagt caaaggaaaa
    16741 gcagatggaa accaagtgaa tgcgattgtg aaagaactat tacaatctta attttatcat
    16801 ttaaactgcg gacagtgatt gtccgcagtt ttttgtgttc gtaaaaaaag aactaaaaaa
    16861 cagctagaag aaagaataag attctttctt ctagctgttt ttttcctgtt taatatctct
    16921 tatgttaaat tgtgtttgat ttggttattc ttctggtagt tcatttgctt cttgtttata
    16981 ccctgatagg cgataggcgt aataaaagag taagctgcca gcgtaaccaa gtaacgttgg
    17041 gaccatccag ccaagtccta atgcagcaaa aggaacgtga tgatgataaa atcctagtaa
    17101 gcttgctacg acagtttgct gactcactaa tgcaggcgaa tttgccaaca tatccaatac
    17161 ggctggaacg gcagaaaaga acatcgttac ttgatagaca aatggactct tttggaagaa
    17221 ttttgctgtt aaggaaagca agattaacgc aagtgacaat ggatacaaaa gcatcaatac
    17281 tggtaaagac cattggatga tattatccaa tccggcattc gcaacgacaa aagagacaaa
    17341 agtcgttaac cgcagccatg tcatataact gactttaggg aacagtttat ggaaatcttg
    17401 tgcaaaagaa acaacgagtc ccatagcggt agtgaagaca cctaatgtga ccattacccc
    17461 taagaagcta gcaccgaagt tacctaaata aaaattagtg atctgagaaa gtgcagttcc
    17521 gccattttct gataaaggca tcttattcaa actcatcgca ccgagaagga ccagaccaaa
    17581 ataaatcatg acttctaacg caacgctcag gctaccagct tttgctgtta attctgtgac
    17641 ttttttatct ttaaaaccaa gatcacgaac tgcatggaca aaagtgacac ttaaagcaag
    17701 taaagctact gcatcaaccg tgttgtatcc ttctaaaacg ccatttgtca aagcgttcgt
    17761 ctgataagaa tctgcagcag attggtttag attccccatg ggttggaata aagaaacgac
    17821 aaaaatgata gctaataatg caagaaacaa ggagtttaaa tactttccga catatttgac
    17881 caaacgtgtt ggatgtacag taaggaaata agcaagtaaa aagaaaagag cagagaataa
    17941 aaacatacct aatgaatcat atttttcagg caaaaagggt ttagcagcca tttcaaaagc
    18001 agttgctgct gtccgcggtg taccaaagaa tggtccgatc gttaaatgaa caaggatcaa
    18061 gaacaaggca gcataccatt ttccgacagg tttagctaag tcatacagac catcactttt
    18121 tgtcactact acagctaaga tagctaataa tgggaaaaga gcgcctgaga tggcaaatcc
    18181 tacaccagcg ctgatccagt ttgatccagc catttgacct aagtggacag gaaagatcag
    18241 attacctgac ccgaagaaca tcccgaagat aagtgaactg accatcaata attgcttcca
    18301 tgaataagtt tttgtttctt ccataaattt ttcctccaat aattatcatt ttgagtaaca
    18361 aaataaacgt tgcagaaact agaaatgcca ctaatatcag attccatgaa aaccaccacc
    18421 ttaaaatata aaaaaaagcc cttttccact tgggaaaagg gctctagatt ctagaacggt
    18481 accacctttt ttcgcttaaa aaagcttctt cattcacgga cgcttttcga taaagttatc
    18541 cgctagcctg ataatggagg cagccacaat gatctacgtt ttcgacattg actcggaaat
    18601 gatgttcgtc tattcgattt attgtcattt ctcacttaca tgactcactg ggaataaatc
    18661 gctgaatagc ctactgtttt cctcgacgct ttgctgtata tttatttgtt gacgttataa
    18721 tacacctgtt ttttttgaaa tgcaagaata tttcaaatat tttgttttct ttttttgaaa
    18781 caagaaaaat acccagagaa tacttgctaa gttagacgga atacttgtcc tctatcttat
    18841 gcatagctta catgatcagc tttttttatt caaacgagac ctttagagaa tggaagcagc
    18901 tattttttaa cgtttcttgg tacttatggt taagcaactc ttttagaacc ttttcttatt
    18961 tgctatatag tttcttctga gtatggtaaa ataggaaaga atgaatcgaa aaaaagggga
    19021 tgtctatttt tgacggaaga gtctagttct ttagagatta gattgacggc tgctgatgat
    19081 gtcagcatgc ttttaggtgc acacgacaag catataaaac tgattgaaga aacaacagaa
    19141 acaaccatcc atacacgtgg agaaatcatt cagatcattg gcgaacaatc ccagatttcc
    19201 ttggctgcct cagtcattcg gacattgcag gaattgatca aacgaggaat ccatgtaagt
    19261 acaccagatg tcgtgacggc actgaaaatg gggcaaaaag gcacattaga ttattttgtt
    19321 gaaatgtatg aggaagagat cgtcaaagac cgtaatggaa aaccaatacg ggtaaaaaac
    19381 agcggccaaa agaaatatgt cgaatcggta gccaaacatg atatcgtatt tggcgtagga
    19441 ccagccggga caggaaaaac ttttcttgca gtagtattag caatcgctgc cttgaaaaaa
    19501 ggtgaagtgc aaaaaatcat tttgacgcgg cctgctgtgg aagcagggga aaatctaggc
    19561 tttcttcccg gggatctaaa agaaaaagta gatccttatc tgcgtcctgt ttatgatgct
    19621 ttataccaaa tttttggatt agatcatacg aaccgattga tggaacgagg cgtgatcgaa
    19681 attgctcctt tagcttacat gcgggggcgg acattggatg atgcattcgt tattttggat
    19741 gaagcacaga acacgacagt tgctcagatg aaaatgttcc tgacacgtct tggttatcaa
    19801 tcaaaaatga ttgtcaatgg agatacgagt cagattgatc tgcctcgagg gacaacaagt
    19861 ggtcttgttc atgcagaacg aacattaaag caaatcaaga aaatcgattt tgttaatttt
    19921 gaagcaagtg acgttgttcg gcaccctgta gttgccgaaa tcatcaaagc ttatgaaaag
    19981 gcagatctcc atcaagaata agctttgctt aggaggataa gcacatgttg aataaaacac
    20041 tgcagaaact acaagaaaaa cttggaaagg cagcattgcc tttcctgctg ttcttgtttt
    20101 ttatcattat ctgcggcgtt acatttagta gtgtcaaaca aaaggccaat gatttcagag
    20161 aaggccaagt agcagaagaa agtatccgtg cgaacaaaac gatcgagaac acggaagaaa
    20221 ccgaacaaaa aagaaaactg gcagcagaag cagtcactcc agaatacact taccaaaaag
    20281 atcttgcaga tgaccagaac aatcggatca agcaactatt tgaattgatc gataaaacga
    20341 atgatgccat caacaaatct tatgacgaaa agattgataa ggcaaaggaa gatgaaaata
    20401 tcccaaagcc tactacagaa gagcggatcg ccgcgttgaa gaaaaatttt gaaaatgtga
    20461 atgcagaaaa tgttgcgttt tatcaaaaac taccgaacaa tttttattcg acgatatttg
    20521 aaatgactga aactgagatc catactgtga gggatgagag tctgaagctc attgacgaac
    20581 agatgagcaa acaagttcgt gaaagtgaat tggaagcttt taaacaagaa gcagaagagc
    20641 aaatccaata tttgaatgtc acaccagcac aacaacaaat gattcgctac ttagtggatc
    20701 aaggtatcgt agtgaatgac gtattgaatg agaaaaagac agaagaattg aaacaatccg
    20761 cacgagaagc tgtacaacca gtgatgatct tccaaggaga aatcattgtt cgtgaaggta
    20821 atcaaatcga tgcttcagct atgaaaaaat tggaattact aggactgact aatcaaacaa
    20881 cttcgatttt tccgctaatt gccatggttt tagctgtttt gcttcaaatc ggcgtactga
    20941 tttataattc acttcaattc caaaatgcag gagaaagaac aaaatacgtg cttttctatg
    21001 tgacagctat gtcagtcagc atagtgctga tgaaattttt ccaattattc caaacggaac
    21061 aagctactta tatcccgctg ttttatcccg ctgcttttgc accacttgtt ttaagctttt
    21121 tcttgaaccg aagagcaggg atcatggctg gtattttcca agcagtatct gcacttttca
    21181 ttttttatga ttcgattggc acgaactttc taacgattat cttgatgtct tatgttttct
    21241 ctgggctcat ggcaacgatt gtcagacgca aacggatctc agaacaagga ttatctgctg
    21301 ccatgtgggt cattattttt cctgtgatga tggatgtcat tttgatcgtc tatcaaggaa
    21361 tgagctttgg tgatgcaacg acttggttaa tgcttgtctg cgggttcact ggtgcactct
    21421 tctcattctt gctgacgatc ggtctacacc catatattga attgatggtc aatgatgaca
    21481 gtgtaattgt attaaatgaa ctcagcaatc caaatcatcc tttattgaaa cagttgctgg
    21541 aagaagcgcc aggtacttat catcatagta tgatggtagc taatctaagt gctaacgctg
    21601 tagcagaaat cggcggacgt tctttgctaa ctcgagtagc ttgctattat catgatatcg
    21661 gcaagatcaa acatgctagc tttttcgttg agaacttgcc ttcaggagcg gaaaacccgc
    21721 acaatttctt attgccagaa gacagtaaac atattatctt tggacatgtg acagatggag
    21781 ccaaaatatt ggaagattat aatatgccgc aaatggtgat cgatatttgc aaacagcatc
    21841 atggaaccac gctaatgcgt tatttctaca tcaaagcgaa agaacgcaat ccggaagtaa
    21901 cagaagagca attccgttat cctggaccaa aaccgcaaag tcgtgaagca gggatcgtca
    21961 atattgctga cagttgtgag gctgcagtac gggcaatgga tcatccgacg agcgaaaaaa
    22021 tcgaaagttt tgtccataat cttatcgaag agcgtatttc cgatggtcaa ttagacgata
    22081 gtggactgac cttgaaagaa atccgaaaag tggaaaaatc attgatcagc ggtttaagta
    22141 gcaccttcca ttctcggatc aaatatccta aaatgaaaac agaagttgag aagataaagg
    22201 aagaacaaga agaatagaaa gaagcgatgt ataatggata ttacctttat ggatgaaaca
    22261 aaccaagtct cagaagagaa aatgaaagaa atcgatgact tgctgcaatt tgcagcatct
    22321 tatttaaagc tgccagagga aacagaaatg tctgttactt tcatggacaa tgcggcaatc
    22381 caagtaatca atcgtgatta ccgtggaaaa gatgcaccga cagacgtcat cagttttgct
    22441 ctggaagaag aaggcgaaga cgaaatccca gtgatttttg aagatggaga gaatccattg
    22501 cctcgtgtac taggagacat catgatttct attgaacgtg ctgctgaaca agcagaagct
    22561 tacggccatt catatgatcg ggaacttggc tttttagcgg tacatggatt cttgcatatc
    22621 aacgggtatg accatatgac accagaagat gagaaagaaa tgtttggttt acagaaagag
    22681 atcttggatg cctatggact caaaagataa aaacgtagag aaaaacaaac atttcctgat
    22741 gtctgtggaa tttgctgtcc aaggagtcaa aaccgttttt gaggaagaac gcaatatgag
    22801 gaaacatgtt gcgttcggta tccttgcttt atttatgggc gtgattttcc agcttgatcg
    22861 gatagagtgg ttatggctgc ttttagctgt ttttctcgtt tggatagtag agatcatcaa
    22921 tactgttttt gaaaatgtag tggacatgtt tacggatttt cattttcatc cgattgggaa
    22981 aaaaatcaaa gatatggcag ctggagcagt tttagtgacc gcttgttttg ctgtcatcat
    23041 tgggttgatt ttatttgtcc ctaaaatata tcatttgttg tttaattaaa aaggagaacg
    23101 tatatgccag aacataaatc agggtttgtc gcaatcgtcg gccgcccgaa tgtaggaaaa
    23161 tccacattat tgaaccgaat cgtgggtcaa aaaattgcca ttatgagtga caaggcacag
    23221 acaactagaa ataaaatcca aggagtatat actacaccag aagctcagct gatttttatc
    23281 gatacaccag gaatccataa accaaagcat cgtctaggag attttatggt agaagcagct
    23341 tacagtgcgt tgaaagaagt ggatgcagtc ttatttatga tcagtgctga tcaaaaacgc
    23401 ggccggggag acgattttat tattgaacgt ctaaaaaacg tgcattctcc agtttatctg
    23461 attatcaata aaatcgataa gatccatcca gacgagttgc tgggaatcat cgaagattat
    23521 tcgacgcaga tgccttttgc acaagtgatt ccgatctctg ctacagaagg aaacaatgtc
    23581 gaacgcttaa tggatgtatt agtcagtgaa atgccagagg gcccacaata ttttccagat
    23641 gatcaagtga ctgatcatcc tgaatacttt atcgtttctg aattgatccg tgagaaagtc
    23701 ttgtttttaa cacgagacga agtgcctcat tctgtcgcag tcgttgttga ttcgatgaag
    23761 cgaaacgaga atgataaaat ccatatccag gccacgatca tcgtcgaaag agatagccaa
    23821 aaaggaatca ttatcggcaa aggcggaaaa atgctgaagc aagtaggaac gaaagcaaga
    23881 aaggatatcg aaaatctttt aggagacaaa gtatttctag aactttgggt caaagtccaa
    23941 aaagactggc gagacaaaaa agtctatctg caagactttg gctaccgaaa agacgaatac
    24001 tgatattctg aacttcttat aggttaagta tagaaataaa ctggataaaa atccgcacgt
    24061 tgagaaaaac ggcggaagat cgaaacataa ataatctgga tcggcagaat ctgcttttcc
    24121 agatttattt tttaggaggg agaatatgag ccagcttgca gaatcgaaag gcttgatttt
    24181 atctagccgt aattacaaag aaaaagataa attagtcaaa atctttacgg aatcttctgg
    24241 caaaatgatg ttttatatca aaggaatcca tcggcaaaat cagccattag ctccggcttt
    24301 gctccccttt accgaagcag tctatatcgg taattttcga agtgaaggtt tgtcgttttt
    24361 aagcagtgtc aaagacgtcc atgcgtttcg gaacatccaa gaagatatct ttttgagcgg
    24421 ttatggtacc tatttgctaa atctagtaga tgcagcaatc gaagatcatc aatatgaccc
    24481 gcatttattc caatttacgc aacaagctct gcagcggatg gatcaaggag atgatgcaga
    24541 aatcatcacg aatatctttg aagttcaaat actgcaacgc ttcggaatcg cgccaatttg
    24601 gacgcactgt gtcgtatgcg gggaaacaaa aggaaaattt gactattctt ccaaatctgg
    24661 cggtgtgata tgcgagaaac actggccaat ggacgaacac cgttatcatg cagatcctcg
    24721 cgctgtttat ttcgttcgaa tgttttcggc tatttcttat gataagatca gtggaatcca
    24781 cttgaaagaa gaaacgaagc aggcaatccg tgacctcatt gaccagttgt atgatgaata
    24841 tgtcggtatt catctaaaaa gcaaaaagtt cattgatcaa atgaagtctt gggcacacgt
    24901 attaaagacg ccagagaaaa atatgcctga aaaagagata cctgaaaaag aagaataaat
    24961 gaaagataca acaaaaaaat caaaactatt tgacaaatac agggacttcc gtctattatt
    25021 aaccgtagat taaatagatc gttcactgag aggatctatt atataaacaa gaaaactgca
    25081 aaatgaatac gtacattgac caagatcagt aaaaaacacg tggtttagcg agttcgggag
    25141 agtggaagcc ggacccaaac gatttttgaa gaggcacttg cgagtacgat aaatgaagct
    25201 gccgaatctt tattcggaag tagggtggaa ccgcgaaatt attcgtccct atgtctgtaa
    25261 tcagacatag ggactttttg tcgttttctt tcctgtattc ttattgacag caacgcttaa
    25321 aaattgaaag gggcaaaaca gatgagtaaa aaattgaccg tccaagatat gattttaaca
    25381 ttacaaaaat tttggtcatc aaacggctgc atgctgatgc aagcttatga cacagaaaaa
    25441 ggagcaggta cgatgagccc atatacattc ttacgggcaa tcggaccgga accatggaat
    25501 gctgcttatg tagagccatc ccgccgacca gctgacgggc gttatggaga aaacccaaac
    25561 cgtttgtatc agcatcatca atttcaagtc gtaatgaaac cttcacctga aaatatccaa
    25621 gagctttatt tagaaagctt gcgtctttta ggaattgatc cattagaaca cgatattcgt
    25681 tttgttgaag acaactggga gaacccatcg atggggtgtg ccggtgtggg ttgggaagta
    25741 tggcttgatg ggatggagat cacacaattc acgtacttcc agcaagtcgg aggactacct
    25801 tgtaaaccag ttacttctga gatcacttat ggactagaac gtcttgcttc atatattcaa
    25861 gaagttgaaa gtgtctatga tctggaatgg gctgatggtg tgaaatatgg cgagatcttc
    25921 aaacaaccag aatatgaaca ttccaaatat tcttttgaag taagtgacca agacttgcta
    25981 ctaagcaact ttgaccgttt tgaaaaagaa gctaaacgct gtatcgatga aaatcttgtt
    26041 catccagcgt atgattatat tttgaaatgc agccatacat tcaatttatt agatgctcgc
    26101 ggtgcagtat ccgtaacaga acgagcaggt tatctttccc gtatccgcaa catggctcgt
    26161 gctgtagcca aaatctttgt ggcagaacgc gaaaaattag gattcccttt attgaataaa
    26221 aacacagcaa atccagaaaa catgaaggag gctgaataag atggcaaaag acttattact
    26281 tgaaatcggg ctggaagaaa tgcctgctca tgttgtcaca ccaagcatga aacaattaga
    26341 agaaaaaaca gcaaaatttt tagatgaaaa ccaattgact tatgatacga tcgaaacgtt
    26401 ttctacaccg cgccgcttag caatcaaagt aacaaacatt ccagaacgtc aagcagacag
    26461 tgaagaagaa gtcaaaggac cagccaaaaa aattgctttg gacgcagatg gaaactggac
    26521 gaaagcagct gaaggatttg tccgcggaca aggtctgacg acagatgcta tcacttttag
    26581 agaattaaaa ggcgtggaat atgtctatgt aacgaaatac actcacggca aagaagcgaa
    26641 agaagtattg acaggtttag cagatgtcat taaaagcctc actttcccag taacgatgca
    26701 ttgggcagaa tacgattttg aatatatccg ccctattcac tggatcgtgg cattgttaga
    26761 tgacgaagta atcccgtttg agatcttaga tgtcaaaaca ggcagagcct ctcgaggaca
    26821 tcgtttctta ggcgaagatg tgacatttgc gcatgcaaaa gaatatgaag aaaaattgaa
    26881 tgaacaattc gttattgctt caccgcaaaa aagacaagaa atgatcgtta cacaagcaaa
    26941 tgaattagcc gaaaaaaatc actgggttat tgatctagac gaagatttac tagaagaagt
    27001 caacaattta gtcgaatatc caactgtctt ttctggcgac ttcgaagaaa aatatctttc
    27061 tgttcctgaa gaagtactag tcacttctat gaaagaacat caacgttatt tcgaggtacg
    27121 gaacgatcaa ggtatgctgc ttcctcattt tatttctgct cgtaatggtg atcgggtaaa
    27181 attggaaaat gtggttcgag gcaatcaaaa agtattgacg gctcgtttag aagatgcgga
    27241 attcttttat aatgaagaca agaaagcttc aatcgaccaa tacgttgaaa aactaaaaac
    27301 tgttactttc catgaaaaaa tcggtagtat ctatgagaaa atgcagcggg tacaagtcat
    27361 tgcccgtatc attggcgaac aagtaggact atctgaggaa gaattgatgg atctgcaacg
    27421 agctgctgaa atctataaat ttgatctagt aaccaacatg gtaggggaat ttcctgaact
    27481 acaagggatc atgggcgaaa aatatgctct gctgaaaggc gagaaacctg ctgtagcaac
    27541 agcgattcgt gagcattaca tgcctatatc cagtgaagga gagctacctc agtctacagt
    27601 aggttctgtt ttagcgattg cagataaatt agatagcatt ttttctttct ttgcagttgg
    27661 gatgattcca agcgggtcaa acgatcctta tgccttacgt cgccaagcat acggtgtcgt
    27721 tcggatcatc gaatcgaaaa aatgggcctt tcctttgtct gttctgcaag aaacgattag
    27781 tgatgttatc agtaaagata cggatcgttt cggcattggt ttatcagaag ggcaacaaca
    27841 ggtgatcgac tttatcaaag gccgtctgcg ccaattattg acaacgaaga atattcgtca
    27901 tgatgtcatc gaagctgttt tgaatgcaga acaaaaagat ctgacaaaag tatttgcagc
    27961 agcacaattg ttcaagcagc atcttgcaga tgaagacttc aaaccatcga tggaagcttt
    28021 gacacgtgtc gtgaacttag cgaaaaaagc ggagcttgaa aaacaatcag aagtagaccc
    28081 tgaactgttc gaaaatgaag cggagaaaga actgcacaaa gcagtagagg caatgaaaaa
    28141 aagctttgct gaccaaacga tcaacgaaaa ttatgatcag cttgtaagcc ttcgcccact
    28201 gatcgaaaat tattttgatc agacgatggt catggcagaa gaaccagcag tacgtaagaa
    28261 ccgattaacg caattaagtc aaattgctgc catggctttg tcgctagcaa gtttagatca
    28321 aatcatcaca aaataatttc taatatgtaa ataaaccaga gaataggcac aaaa
//
LOCUS       DARDHD010000015        28349 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000015 DARDHD010000000
VERSION     DARDHD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.015"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1414
                     /gene="efmA"
                     /locus_tag="SDA06_000556"
     CDS             128..1414
                     /gene="efmA"
                     /locus_tag="SDA06_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG4227851.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1552..2151)
                     /locus_tag="SDA06_000557"
     CDS             complement(1552..2151)
                     /locus_tag="SDA06_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG4227852.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2555..3676
                     /gene="mnmA"
                     /locus_tag="SDA06_000558"
     CDS             2555..3676
                     /gene="mnmA"
                     /locus_tag="SDA06_000558"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG4227853.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3922..5343
                     /gene="arcD"
                     /locus_tag="SDA06_000559"
     CDS             3922..5343
                     /gene="arcD"
                     /locus_tag="SDA06_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG4227854.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5357..6475
                     /locus_tag="SDA06_000560"
     CDS             5357..6475
                     /locus_tag="SDA06_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG4227855.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6535..7827)
                     /locus_tag="SDA06_000561"
     CDS             complement(6535..7827)
                     /locus_tag="SDA06_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4227856.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7959..8537
                     /locus_tag="SDA06_000562"
     CDS             7959..8537
                     /locus_tag="SDA06_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4227857.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8666..9043
                     /locus_tag="SDA06_000563"
     CDS             8666..9043
                     /locus_tag="SDA06_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG4227858.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8976..11048
                     /locus_tag="SDA06_000564"
     CDS             8976..11048
                     /locus_tag="SDA06_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4227859.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11259..12206)
                     /locus_tag="SDA06_000565"
     CDS             complement(11259..12206)
                     /locus_tag="SDA06_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4227860.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12452..12916
                     /locus_tag="SDA06_000566"
     CDS             12452..12916
                     /locus_tag="SDA06_000566"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG4227861.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12978..14021)
                     /locus_tag="SDA06_000567"
     CDS             complement(12978..14021)
                     /locus_tag="SDA06_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG4227862.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14398..15363)
                     /locus_tag="SDA06_000568"
     CDS             complement(14398..15363)
                     /locus_tag="SDA06_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG4227863.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15597..16442
                     /locus_tag="SDA06_000569"
     CDS             15597..16442
                     /locus_tag="SDA06_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4227864.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            16633..17820
                     /locus_tag="SDA06_000570"
     CDS             16633..17820
                     /locus_tag="SDA06_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4227865.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            17826..18182
                     /locus_tag="SDA06_000571"
     CDS             17826..18182
                     /locus_tag="SDA06_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG4227866.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            18184..18522
                     /locus_tag="SDA06_000572"
     CDS             18184..18522
                     /locus_tag="SDA06_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG4227867.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    18598..18854
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18917..19951
                     /locus_tag="SDA06_000573"
     CDS             18917..19951
                     /locus_tag="SDA06_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4227868.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            19944..20630
                     /locus_tag="SDA06_000574"
     CDS             19944..20630
                     /locus_tag="SDA06_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227869.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            20654..21502
                     /locus_tag="SDA06_000575"
     CDS             20654..21502
                     /locus_tag="SDA06_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4227870.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            21699..22472
                     /gene="sufC"
                     /locus_tag="SDA06_000576"
     CDS             21699..22472
                     /gene="sufC"
                     /locus_tag="SDA06_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG4227871.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            22485..23771
                     /gene="sufD"
                     /locus_tag="SDA06_000577"
     CDS             22485..23771
                     /gene="sufD"
                     /locus_tag="SDA06_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG4227872.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            23768..25003
                     /locus_tag="SDA06_000578"
     CDS             23768..25003
                     /locus_tag="SDA06_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4227873.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            24990..25460
                     /locus_tag="SDA06_000579"
     CDS             24990..25460
                     /locus_tag="SDA06_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG4227874.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            25465..26856
                     /gene="sufB"
                     /locus_tag="SDA06_000580"
     CDS             25465..26856
                     /gene="sufB"
                     /locus_tag="SDA06_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG4227875.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(26951..28093)
                     /locus_tag="SDA06_000581"
     CDS             complement(26951..28093)
                     /locus_tag="SDA06_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4227876.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(28132..>28349)
                     /locus_tag="SDA06_000582"
     CDS             complement(28132..>28349)
                     /locus_tag="SDA06_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227877.1"
                     /translation="EFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITD
                     NINKLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         9188 a         5238 c         6031 g         7892 t
ORIGIN      
        1 tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt attttttgaa
       61 aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg
      121 gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt
      181 ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat
      241 ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg
      301 aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa
      361 aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt
      421 agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg
      481 ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc
      541 atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat
      601 cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt accttatttt
      661 attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa
      721 agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct
      781 gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg catttgtgat
      841 gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg
      901 ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg
      961 tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta
     1021 catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt
     1081 cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact
     1141 gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa
     1201 cttcaactct ttgatgaacc tgacaggtcc aattggatta ttatttgcag gaccattagc
     1261 tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg
     1321 aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact
     1381 ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc
     1441 ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa
     1501 aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct
     1561 atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc
     1621 aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc
     1681 gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt
     1741 aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat
     1801 ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt
     1861 ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc
     1921 ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat
     1981 agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt
     2041 gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt
     2101 tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt
     2161 gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa
     2221 aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata
     2281 gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta
     2341 tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt
     2401 tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa
     2461 ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga
     2521 tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt
     2581 tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca
     2641 aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt
     2701 atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc
     2761 ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc
     2821 cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt
     2881 caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta
     2941 tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa
     3001 taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt
     3061 ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc
     3121 gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca
     3181 atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt
     3241 caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag gactagggat
     3301 cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa
     3361 cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc
     3421 aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa
     3481 attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc
     3541 agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta
     3601 tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt
     3661 tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac
     3721 accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt
     3781 atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt
     3841 ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt
     3901 ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg
     3961 gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg
     4021 gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt
     4081 ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt
     4141 attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt
     4201 tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg
     4261 ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt
     4321 atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc
     4381 aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt
     4441 atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta
     4501 gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt
     4561 atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa
     4621 cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc
     4681 cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa
     4741 cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat
     4801 atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa
     4861 acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa
     4921 aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct attcttattg
     4981 actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt
     5041 ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc
     5101 aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta
     5161 gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac
     5221 tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg
     5281 ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata
     5341 taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg
     5401 ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga
     5461 actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc
     5521 attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta
     5581 tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt
     5641 agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca
     5701 gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga
     5761 agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa
     5821 aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt
     5881 agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata
     5941 tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat
     6001 agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc
     6061 tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg
     6121 cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc
     6181 gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga
     6241 accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat
     6301 cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt
     6361 accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga
     6421 aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa
     6481 aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg
     6541 tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt
     6601 ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag
     6661 aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac tggagccgat
     6721 cataggccct actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc
     6781 agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc
     6841 atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc
     6901 cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc
     6961 aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat
     7021 cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat
     7081 gtacagactg atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac
     7141 gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac
     7201 catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa
     7261 gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc
     7321 agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt
     7381 tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt
     7441 actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact
     7501 agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat
     7561 caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac
     7621 caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt
     7681 acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc
     7741 cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa
     7801 taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac
     7861 agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc
     7921 tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga
     7981 aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga
     8041 tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct
     8101 cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc
     8161 tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac
     8221 tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt
     8281 tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga
     8341 atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc
     8401 gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact
     8461 ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc aataacttta
     8521 tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat
     8581 gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag
     8641 aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga
     8701 gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc
     8761 tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga
     8821 atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg
     8881 gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca
     8941 tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa
     9001 aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt
     9061 attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa
     9121 tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc
     9181 ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc
     9241 agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag
     9301 gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt
     9361 tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg
     9421 cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg
     9481 taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc
     9541 gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc
     9601 gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc
     9661 aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca
     9721 gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat
     9781 tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa
     9841 agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc
     9901 gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag
     9961 cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac
    10021 agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa
    10081 acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct
    10141 ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg
    10201 aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag
    10261 gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg
    10321 atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc
    10381 ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga
    10441 aactaatcaa atctgaactg atggaacgta cccctcaagg actgcaagta tctcctaaag
    10501 gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct
    10561 gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg
    10621 gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc
    10681 aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa
    10741 acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa
    10801 aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg
    10861 aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg
    10921 ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat
    10981 atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga
    11041 agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag
    11101 gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgaagaagct
    11161 acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg tcacagtcct
    11221 ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg cttcaattca
    11281 tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt cgtttctaag
    11341 aagattttcc caatatattt cccgatgatt ccaagacata acaattggat tccaccaatg
    11401 aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa caacaatgcc
    11461 cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc gccgataaat
    11521 gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga gtagttgaat
    11581 aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc ataagaaata
    11641 tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt gtattctgtt
    11701 aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg agcgccatcg
    11761 accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc aaagaaactg
    11821 cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa
    11881 gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag atcaacatcc
    11941 atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc cgcttctttc
    12001 ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc tagcttcttg
    12061 attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc gaactcatgg
    12121 gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa
    12181 catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg tttatatatg
    12241 gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat tttacgtaaa
    12301 agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc tcggtttatc
    12361 aaacttttaa gaacattttt tattagttgg caagtagttt tggatagaga aaaaggagga
    12421 agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta tcggcttttt
    12481 atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa tttcagaaag
    12541 cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag aatattttgc
    12601 gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc cttttcagca
    12661 aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt ataaagaagt
    12721 agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa
    12781 tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa aactgacagg
    12841 atatatggga aacgcaatag atggggttga aatcaaacgc caattgttgt tgttagaagg
    12901 atatctgcaa aattaaaaac agttgtgatt cggacattgt tccatgcgag aatcacaact
    12961 gttttttcat gttcattcta aactttgatc agccaaataa atagtaaagc catcactgtt
    13021 tggagaagcc cgacagctaa tccataagat aagaagcgtg taccttcttt catgaatttt
    13081 gcaaagtcta aacgcagacc aattgccgct aaagcagtga tctcaaacca gctgctgata
    13141 aaatgtgccg attggtcaat aactacaggt aaagaaaaca agctgttgac caaacaaaag
    13201 atcacaaacc ctaatacata ccaaggaagc ggtaattttc gttttttctg cgtatcagtt
    13261 ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt tttcaaacag atagacaaca
    13321 acaacgagca tcataatacg tagaatctta aatagcatgg cgaattcgac aacagaattg
    13381 tcaagtaagc ttgccccagc gactacttga ccaacagatt ggagtgtacc acccaaaagt
    13441 gcactttttt caatgagatt tccaccaaac aactgtaatc caagaaaagg gagagtcaac
    13501 atcatcacag tacccagtag attgactagt gtgatgatct gtcctttttc ttcctctttc
    13561 gcatggatgg aaggggcgat tgaagcaata gcagaagaac cacaaacagc gtttcctcca
    13621 gccatcatca aagaagaagt atcgctgaat tgcagttttt taccaatcaa gtaagcaccg
    13681 atgatagtga ctgacatttg cagtaacaca aaaacaattc cttttacacc aatttgagca
    13741 atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag agaattctaa tagtttgctt
    13801 tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa
    13861 atacccaata aaatagcaat agtcgctcca cctaactgtg gcagataaat agcaagaagc
    13921 ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct
    13981 gaatccttta cagattttat tggatgtgac aatgatttca tttgatcggt tcctttcgtc
    14041 aataaatact aaaagcatac tcgaacatag tagcactaaa ccagtatttt gacaaaagaa
    14101 caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg tgttccagaa
    14161 gtagcttcta cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
    14221 tccttcttat ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa cggtgttcaa
    14281 aagcttgcat ctgcggtaat aagcccaacc agtacaaaaa catgaacagc tgtttttgtt
    14341 tggccgttct tattacttga tgcagaacag ctttttcaca acctattgaa tcgattatta
    14401 agcgattttt ttagaaacaa attttccatg tttgctatgt tctttaatta agaaacgtac
    14461 gtaatgagca agaacgtaga agacaactac acttgcaaac agacattccg caacttttaa
    14521 gaaatccaat acgctactat agatacctaa actgttgaag taatgaacag tagtaaatat
    14581 agctgttgca gagataacaa gcgggaatgt aaaggctgca tagcttggat aaaaacgaag
    14641 cttgaacaaa cgtggaagca tagacagcac aacgaaatac aatacttgag ataaagcgaa
    14701 caatgctacg acaagataaa aattaggttc agcaaagttt ttaagataac ctgttaggca
    14761 aagagaacca ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag ccatatttct
    14821 tacgaaaaag acacgtttga tgatgatcgg caataagaac gcatagcaga tcaatgaaat
    14881 ccaaaagaaa atttttccaa taagtgggaa gaagttcccg gcagttacag tgatgacccc
    14941 aaatccaacg taaatgataa accagcttgg ataaattgct tcaattgaaa gatcagcttt
    15001 gataacatgg tgataattga aataaaagac aaggatcact tggaagataa cagcgcacag
    15061 ccaaatgaat ttgacaattg gtgcaatact ttcatatgaa acaaaataag tacaaataac
    15121 catgagtgac atcgtaaaag ttggtgaaac ggaggcgatg attggatcat gcaacgtttg
    15181 tttcgtgtgt tcgaataaga taaacaattt aacaaaaatg aatatcataa gaatcataga
    15241 gataccgcca gttagattgc ctaaagcaac atggtgatag tctttaaaca gatttccaag
    15301 ggaagctaac ccaaggatca acccgcagat cgggatcgga ataattttca aaaagttctt
    15361 catatataat attctccttt ttatttaata gattttagta ccaagatgaa cgaatacatt
    15421 gtaaaatgaa taggaatgta tgattacgag caagcagaca gctgattgtc ttcatctaag
    15481 ttactagttg gatcagcaac tgttttgttg tacatttcta ttatagatgg ttttttgtat
    15541 atttaaatca aatagttttt atagtaatta tcataaaaag ttatggaagt ggtgccatgt
    15601 ttaagttatt aacgactttt cgagcagtat atgaaacaag aaatttttcc agagcagcag
    15661 aagtactgtt tttatctcaa ccagcagtct cgaatcaaat caaacagttg gagcgggaat
    15721 tgaatatcga tctttttcaa cgtaacggaa gaaaagagat gcttccaact aagcaagccg
    15781 acttattata tgaacggaca ctggttttgt tagaagaatg ggaagaaact cagcaaaaat
    15841 tgctcaatga acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac acctttgctg
    15901 tctatctttt gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg
    15961 agatcgaaat cacgaattct catgaatcat tagaacaagt agcgaaacat gaatccgatt
    16021 ttggttttat cgaaaagcca ttagagactt ccggcgtaca aagatactct ttgatggaag
    16081 accagctagt catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact
    16141 ctggtgtttt tcattacacg cagcgttatt ttgaagagat gaatatccaa gataagaaga
    16201 tgatcgtgaa aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga
    16261 ttttgtctaa gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat ttccatcgat
    16321 atttttattt catccaacga accaatctgg tttctgatca gctgctagag attgccgaag
    16381 agatccttcg atacaatgaa agcacagaaa cttctcacag cgaggagaat ctcagctggt
    16441 agataattgc atgtatcaaa aaaagttcta tgatacacta aagataagca gtaggctttt
    16501 gtactgcatt tgctcaagca actgcacaaa cggaaagtta atttttccca aatgtctatg
    16561 gaaagacttt ccattatcta actggaccgc tataataatc agcagtatgg tttttaagaa
    16621 aagggatgaa acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat tgattatggc
    16681 gtgattttac cagtctttct gttatgtttg attggtctgc tctcattata tgtagcactt
    16741 agccatgatc cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat
    16801 ttagttggag cggcagcaat cgttgtcatc atgcatttga attccaaatg gatctggaaa
    16861 ctgactcctt acctttatgg tttggggtta gtggtaatgg gactcttgct caaattctat
    16921 gatcagaatt tagctgcttc aacagggtct aaaaactggt ttcgattcgg tgcgtttact
    16981 ttccagccag cagagttgat gaagattgcg tacatcttga tgatggcact gatcgtaact
    17041 aaacataata cgcaagtaaa agaacggacg atgaaatcgg acttttggct gattgggaag
    17101 ctcttgatcg tgacaatacc ggttttagca ttgatcatgg cccaagatga ctttggaacg
    17161 atgcttgtct ttcttgcgat ctttggcggg attttcctga tgtctggaat ctcttggcgc
    17221 atcatcgcac ctgtagtaat cttggcagta gtcgttggag caggaacgat cttcttggtt
    17281 acgactgaag gcggaagaga cttgctttat aaagttggat tcaaatctta tcagtttgca
    17341 cgtattgatt catggctgga ccctttccat gatacgtcag gaatgagtta tcagccggcc
    17401 caaggacttt tggcaattgg taccggtggc ttgtttggta aaggattcaa cgtaagcaat
    17461 atctatgtgc ctgttcgtga gtccgatatg atttttaccg ttatcggtga aaacttcggc
    17521 tttatcggtg gtgcattcgt cattttcttg tattttattt taatctatcg tatgattcgg
    17581 gtgtgtttcg atacgaataa tgaattttac gcctatatcg caagcggttt gatcatgatg
    17641 ctgctgttcc atgtctttga gaatattgga gcaaatatcg gattacttcc tttaacaggg
    17701 atcccattgc ctttcattag tcagggagga tcctcgattt tagggaacat gatcggtatc
    17761 gggttgatat tatcgatgag ataccaaaat gaagcacctg caagatcttc aaggaggtaa
    17821 gaaaattgta caccttttac tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt
    17881 ggttagacca gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg
    17941 cctcaacgat tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca
    18001 caagcgggat gaaatatcgg gaacttggac tgaaagatca agtaggcgag tttacggctt
    18061 caaaagcgag ccaagtctta tcgacagatg gtatgctgat caagcgacca ctgatcttac
    18121 gagaggacca gcttgttgca attggattca atgaaaaaat ttatgaagga gaactgatat
    18181 aaaatggcag gcaagcattt gaagaaaaag gacaaccttt ggatcttatt taatggaaaa
    18241 gaatactgtg ttggtttgac aaatgaagcg caagaagaac ttggagacat cacgtttgcc
    18301 aatctgccaa aagcaggaca atcgctaaaa aaagatgaac ctctgatcga agttgaagcg
    18361 gaaaaagcgg tcaatgaatt tgccagcccg ctaacgggta cagtttcttc tgttaatgat
    18421 aaaatcggtg aagatatcaa tatcttgaat gattcagatg aaatgaatgc gtggatctta
    18481 agtttcaaag atgttgatcc aaaagaattt gacgaattat aaaatcagaa aatcacttga
    18541 aaaaagttga cactgattct tccagttgct ataatcataa gcaatcctat atgaaacgag
    18601 aaagctttga aaagaagagt acatgtcacg gacgtttgaa gagagccctg tttgctgaaa
    18661 atgggtaacg aaaatgagat ggaagatggt cttagagcag agtaagcgag cgtaagcaaa
    18721 cttactacgg gagtgcccgt tacagcgcga cagtatacgg aaagaagcta agtccaagta
    18781 ctggaagagg ctgttattgc gaagtaacgg taaatcaagg tggtaacacg gaagttcaag
    18841 ctttcgtcct tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa aatcataaga
    18901 gaaagatgtg aatggcatgt cattaatcca actaacgaat gtcaaaaaga cattcaatgg
    18961 gaaaaacgga aacatccaag ccgtcaatga cgtttcacta aacgttgaaa aaggggatat
    19021 ttatggaatc gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg
    19081 acttgagctt cccacgagcg gcgaagtgat cgtaaacgat caagatatta caaaattaaa
    19141 aaacaaagaa ttacgtacat tccgaaagaa aatcggtatg atcttccagc atttcaacct
    19201 gttatggtca agaactgtac ttgaaaatat ccagctgccg ctggaattag ctggcgtacc
    19261 aaagagcaaa cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt tagaaggacg
    19321 cggacaagca tatccttcac aactttccgg cggacagaaa caacgcgtcg gtatcgcacg
    19381 agcattggca aatgatcctg agatcctgct ttgtgatgag gctactagcg cattggaccc
    19441 gcaaacaaca gaagaagtat tagacttatt gttagcgatc aataagaaat tgaatttgac
    19501 catcgtcttg atcacacatg aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt
    19561 gatggagttg gggaaagtcg tagaagaagg agacgtattg actgtcttca gacaaccgaa
    19621 aaaagaagta accaaacgct ttgtacaaca agacatcgaa ccagaagctg attcgacaga
    19681 actattagct gaactcatca aagaaaatcc aagcggccgt ttggcaactt tgacctttag
    19741 cgaaagaaat gccaacgagc cagtcatttc ccaagcgatt cgacgctata acatcgatgt
    19801 caacgttgtg tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt cattgactgt
    19861 cttgatgact ggtgaaacca aagaattaga tcaagcagaa gtgttcatca aagaacaagg
    19921 agtaggaatt gaggtgatcc atcgtggata aaagcttttc agaaacatat ctagatttta
    19981 aacaaatcaa cccggaaacg atgaatcagg ctatccttga tacactatat atgacattaa
    20041 tctcaatggt cttagtgacg atcatcggat tagttttggg attgatcctt tactctattg
    20101 gacgcaaaaa caaaccgtat gctcgaatca tttatggaat cgtatcgatc atcagtaata
    20161 ttttccggtc aacgccattt atgatcctga tggttttgat cattccattt actaaagcaa
    20221 tcgtaggcac tatgctaggt gcgcaagcag ccattccagc tcttgtttta tccgcagcac
    20281 cattttatgc ccgtctagta gagattgctt ttcgtgaagt cagccctggt gtactggaag
    20341 cagcagatgc aatgggagca agttactggg aaatcatctg gaaggtcttg atacctgaaa
    20401 gtattcccgc attgatttct ggattaacag ttacaacgat ctcgatgatc ggatttactg
    20461 cgatggccgg cgcgatcggc gcaggtggac ttggaggtct tgcttggcag gaaggctacc
    20521 agcgtggtaa tctaacagtg acattcgttg cgactttaat tattttacta atcgtattta
    20581 tcgttcaagg aatcggtgat ttcttaacta agaaaacaga caaaagataa taggatatag
    20641 ggggatgtag gatatgaaaa agaaaatttt tggatttgca gcagcgcttt tgctgactgt
    20701 aggattagca gcatgcggta acaacagcga cagcaaagat tcagcaaata aagcagacga
    20761 cacgacattg aaagtcggcg cttcaccgac accgcacgca gagattttgg aacatgtgaa
    20821 accattattg aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg actatgtatt
    20881 accgaaccaa tctttggaag aaggcgacat tgatgccaat tacttccagc atattcctta
    20941 tttgaacaaa gaaatcaaag aaaaaggata tgactttgtc aatgccggtg ctgtccacat
    21001 cgaaccaatg ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc
    21061 aacaatcatt acttcaaatt ctgaatcaga ctggggacgc atcatcacaa ttctacaaga
    21121 tgctggctta gtcaaagtca aagatggagt agatctggaa acagcaacat tcgatgatat
    21181 tgaagaaaac cctaagaact tgaagttcaa ccatacgatc gacccatcac ttttagcttc
    21241 tacgtaccaa aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc
    21301 agggttgaat ccattagaag atgccgtatt gcttgaaaag gacaactctc cttacgtgaa
    21361 tattattgcg actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt
    21421 gcttcaccgc aaagaagtcc aagaatggat cacagacaaa tggggcggct ctgttaaacc
    21481 agtaaaggct gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg
    21541 gcttatttct catctttttt atacctattc taatttagaa atcaaaattg tttaataata
    21601 aataaaagat atttgatttt agtaaggaag gtcattgcgc ttcattctca attagtctaa
    21661 aatgagtgag aacgaaagag aatttggggg tataatgcat gtctgtctta gaaataaaaa
    21721 atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa
    21781 ccggagaaat ccatgccatc atgggaccta acggcacagg aaaatctacc ttatctgccg
    21841 ctatcatggg caatccaaac tatgaagtaa cagaaggcga aattttgttt gacgggaaaa
    21901 atgttttaga cttggaagta gatgaacgtg cgcgacttgg cttattttta gctatgcaat
    21961 atccaagtga gataccaggt atcacgaatg cggaattcat gcgtgcagcg atcaatgcga
    22021 aacgtgatga aaacgacaaa atttctgtta tggcattttt gaaaaagtta gatgaaaaaa
    22081 tggctttact aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg
    22141 gcggagaaaa gaaacggaat gagatcctgc aattgttgat gttagaacct acatttgcaa
    22201 ttttagacga aatcgattca ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag
    22261 taaatgaaat gcgcggagac aatttcggtg cattgatcat cacccactat caacgattat
    22321 tgaactatat cacacctgat gtcgttcaca tcatgatgga aggccgagta gtcatgacag
    22381 ggaatgctga cctagctaaa cgtctggaag cagaaggcta cgctggtatc agtaaagaat
    22441 taggaatcga ctataaagaa gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact
    22501 ggctcgatta tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa cctgattgga
    22561 tgagaacctt ccgtcaagac gcactagcga aagcagatga actgccattg ccgcatatcg
    22621 atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa agaaacacaa accatcagtg
    22681 aaacaggcac gattccggca tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag
    22741 gttcatggac gatttttgaa caactgcctg tggaattagc agaaaaagga gtcattttta
    22801 ccgacttgtt cactgcaatg atcgaatatc cagaactagt ccaagaatat tatatgaaaa
    22861 aagcagtaaa catgaacgaa gaccaattga ctgcactcca tgtagctttt atgaacagcg
    22921 gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga accactcgag tccttattca
    22981 ttcaagatgg cgcatccgat gagcacttct tcaagcatgt gttgatcgtg gcagatgaac
    23041 atagtgaatt ttcttatctg gaacgtttcc agacaaccaa agaacaagta gcaaaatcat
    23101 caggtaatat catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa tattcagccg
    23161 tagatcaatt aggagaaaat attacttctt atatgaatcg ccgtgggcac attttacgag
    23221 atgcttctgt cgactgggca attggtgtca tgaatgacgg tcatgtgatc gctgactttg
    23281 actcagattt agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg
    23341 gcagacaaat ccaaggaatc gatacacgag taaccaataa agcccctcat acgatcgggc
    23401 atattttgca acacggagtg atccgagaaa aaggaacatt gacctttaat ggtatcggcc
    23461 acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt
    23521 ctgataaagc acgtggtgat gcgaatccta ttcttttgat cgatgaaaac gaagtaactg
    23581 ctggacatgc tgccagtgta ggacgagttg atccagaaga aatgtactat ctgatgagta
    23641 gaggattgca taaagaagaa gcagagcgct tagtgattcg aggattcctc ggttctgtct
    23701 taacagcaat ccctgtagaa caagttcgga aagaattagt cgaagtaatc gaagggaagt
    23761 tgaatggatg atcgatgcta aaaagctacg caatgatttt cctatcttat ttcaagacgt
    23821 caatgatgaa cctttagttt atctggataa tgcagcgacg acccaaaagc caaaacaagt
    23881 actccaggca ttagcgtact attacgaaca cgataacgcc aatgttcatc gcggtgtcca
    23941 tacgttagca gaacgcgcga cccatgatta tgaacaagca agagaaaaag tacgggcgtt
    24001 catccatgca aaagaaacag cagaagtgct gtttactcgt ggtacgacga caagcttgaa
    24061 ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca ggagacgaga tagtgctctc
    24121 ttacatggaa catcattcta atgttatccc gtggcagcag ttggcaaaac gaacaggtgc
    24181 tgtcttgaaa tatatcgaac taacagaaga aggcttcctg gatatggagg atgcagcgaa
    24241 aaagattacg gacaaaacca aaatcgtctc cattgctcat gtatccaatg tcttaggtgt
    24301 cttcaatcct gttcaagaat tagctgaact tgtccatgaa aacggcgggg tcatcgtagt
    24361 agatggtgct caatctgccc cgcacatggc cattgatgtc caagcactag actgtgattt
    24421 ctttgctttc agcggacaca aaatgtgcgg accaacaggt attggtgttt tatatggtaa
    24481 acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga tcgattttgt
    24541 cgaattatac gacagtacat ggaaagaact accttggaaa ttcgaagcag ggacaccaaa
    24601 tatcgccggc gctatcgcat taggccatgc aatcgattat ttgcaaaaac tcggtatgga
    24661 caatatccat cgatatgaag aagaactagc cgcttatgtg ttgccaaaat tacaagcaat
    24721 tgatggattg actgtttatg gtccacaaga cccgaatttt cggacaggag tcgttgcatt
    24781 caatttggat gggttacatc cccatgatgt cgcgacagct ttagacatgg aaggggtagc
    24841 cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag taccttgaag tatcagcgac
    24901 tgctcgcgcg agcttttatt tctacaacac aacgcaagac gccgaccgtt tagttgaagc
    24961 aatcatggca acaaaggagt ttttccaaca tgggactatc taaattagac aatttatacc
    25021 gtcaagtgat cttggatcat tcttcccatc cgcatcatcg tggaagcttg actgactcga
    25081 caagcaagat cgaattgaac aaccccacat gtggagatgt gatcgaattg caagtcgatg
    25141 taaaagacgg aatcatcaaa aatatcgcct ttgacggcag tggctgctcc atcagtacgg
    25201 caagtgcctc gatgatgaca gacgccgtca tcggaaaatc aatagaagaa gcagaacatt
    25261 taactgtaga ctttctacag cttgtccaag gaaaagaagt caaagatccc gaccaattag
    25321 gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac
    25381 ttgcctggaa agcactagga aaagcaatgg aaaacgatgg taccgcagct ttccatcatt
    25441 accacaagga agaggagtga gaacatgggc gtaccagaat tagaagaata taaattcggg
    25501 tttcacgatg atgtcgaacc ggttttcagt acaggagaag gcttgacaga agaagtgatc
    25561 cgtgagatgt caagaatcaa aggcgaacca gaatggatgc ttgaattccg tctgaaatct
    25621 ctagaaacat ttaacaaaat gccaatgcaa acatggggcc ccgacctttc agacatcgat
    25681 ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac
    25741 gtaccagaaa aaatcaaaga tacatttgaa aagattggta taccagaagc tgaacgagct
    25801 tatctagccg gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa tatgaaagat
    25861 gaattcgaaa agttaggaat cgtctttact gacactgact ctgctttgaa agaataccct
    25921 gaattattca aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg
    25981 ctgaattcag ccgtatggtc aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc
    26041 gatgttccat tacaaacgta cttccggatc aatgcggaaa acacggggca attcgaacgt
    26101 acgctgatca ttgttgacga aggagcaagc atccactatg tcgaaggttg tacggcacct
    26161 acttattcaa gtaacagcct gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg
    26221 tattgccgtt acacaacgat ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa
    26281 cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa
    26341 acaacgatga aatacccaag tgtttactta gatggaaaag gtgcacgagg caccatgctc
    26401 tccatcgcct ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat gatccacaat
    26461 gcaccaaata catctagctc tatcgtctct aaatcaattt caaaagacgg cggagaagta
    26521 aactatcgag gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa
    26581 tgtgacacga tcatcatgga cgaatggtcg aaatccgata cgatcccatt caatgagatc
    26641 cacaacagcc aagtctcttt agaacacgaa gccaaagtct caaaaatctc agaagaacaa
    26701 ctctactatc tgatgagccg aggcctgacc gaatctgaag caactgaaat gatcgtcatg
    26761 ggctttgttg aaccattcac caaagaactt ccaatggaat atgcagtcga actcaatcga
    26821 ttgatcagtt atgagatgga aggcagtgtt gggtaaacct gttataggag ctagaaccct
    26881 tgatatataa gggttctagc ttttttgttt tgcctgtata ctagaaatga ccagattttg
    26941 accagctctt ttactgatga gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg
    27001 cattatttgt aatatgtgca tagatgtcta atgtcacttt actattttca tgccctaatc
    27061 gttgttgaac taccttgaca gattctcctg cttctaataa taaactagca tgtgtatgtc
    27121 taaacccatg aggtgtaata cgaggcaagt tatatttttt caaaatatat cttaaccaat
    27181 cattaacagt gtttggaatg tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt
    27241 tagagctagt attgtatcca agttttaaat actcttcttt ttgctgtaat ttccagttgt
    27301 tgaggataga aagagtctca gtatctaaag agataactcg tctagaagct cttgttttcg
    27361 gagattgaat tataacttta ccaaattcat ctacagcaag agttttattg acatgtagtt
    27421 ctttattaaa cgtgtctatg tctttccact gtaaagcgag tacttcactc tttctcatac
    27481 ctgtgtatgc caataatctg aagaaggcgt attgtttcat gttaccaaaa tctttaaaag
    27541 catccagtaa attattaagc tcctcttttg agtaaaattt acgatttttc tcatactctt
    27601 tttttcgtgg aagaagtgtt ttttccatag gattattgtc aatatattgc attgcaaccc
    27661 cgtagcgtaa aatttgtgca gtctgttttc tcatataatc gtaactttca tatttactgt
    27721 gccattcatc taccactttt tgacaatatg ggacagagat attcgatagt ttaagcttac
    27781 caaatgctgg aagtatttga ttatttgcgt atcttactga gattgcgatt gttgaagctt
    27841 taacactggt acgctgatga gttatccatt cattatacag gtcttcaaat gtcatatcag
    27901 gtgctttctt tttaacaccg tataatattt gatgtctgta ctcgtcataa tctttttgag
    27961 cttcttgttt gctactaaaa cctcttctcg tagtatttat tttttcacca gttcgtggat
    28021 cggtggcaat gtgtccttta tacatccacg ccacttgacc gtttttcttt ttgtactttg
    28081 atatttttgc cattaaatca tctcctctta gtttacaagg agtacttacc actagatggt
    28141 atcacctccc ttcatttttt tcttagtgta ttttttccca attgatgaaa tcgtttgttc
    28201 tagcttgttt atattatcag taatttctct cacagcttct tcgggataag acatgtcttt
    28261 caattgaata ggtagccatt catctaccat tctttctagt agttcttcta tactgctagt
    28321 gaggaaaaga atgttattgt caaattctt
//
LOCUS       DARDHD010000016        28001 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000016 DARDHD010000000
VERSION     DARDHD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.016"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SDA06_000583"
     CDS             8..649
                     /locus_tag="SDA06_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227878.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SDA06_000584"
     CDS             708..1148
                     /locus_tag="SDA06_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227879.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SDA06_000585"
     CDS             1160..1828
                     /locus_tag="SDA06_000585"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4227880.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SDA06_000586"
     CDS             1892..2515
                     /locus_tag="SDA06_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG4227881.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SDA06_000587"
     CDS             2571..2957
                     /locus_tag="SDA06_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG4227882.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SDA06_000588"
     CDS             3170..4561
                     /locus_tag="SDA06_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG4227883.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SDA06_000589"
     CDS             5172..5540
                     /locus_tag="SDA06_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG4227884.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SDA06_000590"
     CDS             5720..6661
                     /locus_tag="SDA06_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG4227885.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SDA06_000591"
     CDS             6730..7461
                     /locus_tag="SDA06_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG4227886.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SDA06_000592"
     CDS             7605..9785
                     /locus_tag="SDA06_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG4227887.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SDA06_000593"
     CDS             9805..10287
                     /locus_tag="SDA06_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG4227888.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SDA06_000594"
     CDS             10303..11064
                     /locus_tag="SDA06_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4227889.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SDA06_000595"
     CDS             11195..12529
                     /locus_tag="SDA06_000595"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG4227890.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SDA06_000596"
     CDS             12836..13321
                     /locus_tag="SDA06_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG4227891.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SDA06_000597"
     CDS             13314..13997
                     /locus_tag="SDA06_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG4227892.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SDA06_000598"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SDA06_000598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG4227893.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SDA06_000599"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SDA06_000599"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG4227894.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SDA06_000600"
     CDS             17613..18242
                     /locus_tag="SDA06_000600"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG4227895.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SDA06_000601"
     CDS             18294..18626
                     /locus_tag="SDA06_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4227896.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SDA06_000602"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SDA06_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG4227897.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SDA06_000603"
     CDS             19452..20825
                     /locus_tag="SDA06_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4227898.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SDA06_000604"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SDA06_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG4227899.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SDA06_000605"
     CDS             21880..22320
                     /locus_tag="SDA06_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4227900.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SDA06_000606"
     CDS             22464..23165
                     /locus_tag="SDA06_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG4227901.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SDA06_000607"
     CDS             23162..24283
                     /locus_tag="SDA06_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG4227902.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SDA06_000608"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SDA06_000608"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG4227903.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SDA06_000609"
     CDS             25662..26570
                     /locus_tag="SDA06_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG4227904.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SDA06_000610"
     CDS             26605..26877
                     /locus_tag="SDA06_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG4227905.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SDA06_000611"
     CDS             26888..27934
                     /locus_tag="SDA06_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG4227906.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9411 a         4844 c         6101 g         7645 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg t
//
LOCUS       DARDHD010000017        27770 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000017 DARDHD010000000
VERSION     DARDHD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.017"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(240..1184)
                     /locus_tag="SDA06_000612"
     CDS             complement(240..1184)
                     /locus_tag="SDA06_000612"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4227907.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1480..2424)
                     /locus_tag="SDA06_000613"
     CDS             complement(1480..2424)
                     /locus_tag="SDA06_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227908.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2645..3859
                     /locus_tag="SDA06_000614"
     CDS             2645..3859
                     /locus_tag="SDA06_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4227909.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3881..5896
                     /locus_tag="SDA06_000615"
     CDS             3881..5896
                     /locus_tag="SDA06_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG4227910.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5979..7352)
                     /locus_tag="SDA06_000616"
     CDS             complement(5979..7352)
                     /locus_tag="SDA06_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4227911.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7378..7662)
                     /locus_tag="SDA06_000617"
     CDS             complement(7378..7662)
                     /locus_tag="SDA06_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4227912.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7664..9679)
                     /locus_tag="SDA06_000618"
     CDS             complement(7664..9679)
                     /locus_tag="SDA06_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4227913.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9858..10163)
                     /locus_tag="SDA06_000619"
     CDS             complement(9858..10163)
                     /locus_tag="SDA06_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG4227914.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10252..11283)
                     /locus_tag="SDA06_000620"
     CDS             complement(10252..11283)
                     /locus_tag="SDA06_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4227915.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11490..12365
                     /locus_tag="SDA06_000621"
     CDS             11490..12365
                     /locus_tag="SDA06_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4227916.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12517..13359)
                     /locus_tag="SDA06_000622"
     CDS             complement(12517..13359)
                     /locus_tag="SDA06_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4227917.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13451..15328)
                     /locus_tag="SDA06_000623"
     CDS             complement(13451..15328)
                     /locus_tag="SDA06_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4227918.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15325..16746)
                     /locus_tag="SDA06_000624"
     CDS             complement(15325..16746)
                     /locus_tag="SDA06_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4227919.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16749..20138)
                     /locus_tag="SDA06_000625"
     CDS             complement(16749..20138)
                     /locus_tag="SDA06_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG4227920.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20395..21786
                     /locus_tag="SDA06_000626"
     CDS             20395..21786
                     /locus_tag="SDA06_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4227921.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21859..22506)
                     /locus_tag="SDA06_000627"
     CDS             complement(21859..22506)
                     /locus_tag="SDA06_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4227922.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22514..23200)
                     /locus_tag="SDA06_000628"
     CDS             complement(22514..23200)
                     /locus_tag="SDA06_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4227923.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23206..24279)
                     /locus_tag="SDA06_000629"
     CDS             complement(23206..24279)
                     /locus_tag="SDA06_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4227924.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24501..25175
                     /locus_tag="SDA06_000630"
     CDS             24501..25175
                     /locus_tag="SDA06_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4227925.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25180..26520
                     /locus_tag="SDA06_000631"
     CDS             25180..26520
                     /locus_tag="SDA06_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4227926.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26669..27655)
                     /locus_tag="SDA06_000632"
     CDS             complement(26669..27655)
                     /locus_tag="SDA06_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG4227927.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8092 a         5247 c         5043 g         9388 t
ORIGIN      
        1 ttgtatttgc cgttgcggaa ggatttttta attaaccaca gataaacatt gataacgatt
       61 cgcttattgt aatcaacaga ttaaatttca tatgttcata tgtacggata cattttcgga
      121 ccgattaatt ttaaccattc tgtttatttg cttacctatt agatatcaat aaaaaactgc
      181 ttaacagcta tttcagctgt taagcagttt taggattttt agttcatatt cattgtttct
      241 tataatacag tgttcaatac ttctcttaaa gcacttgttg atttgctgaa cattgctttt
      301 tcttcttcgt tgattgataa ttcaacaact tcacgaacac cattttcgtc aacgattgat
      361 ggcacacctg taaagatatc tttttcgtcg tattcaccag ttaaataagc agatacaggc
      421 aatacagctt gttcgttatt caagatagct tttacgatac gtgcagtact cataccaata
      481 ccatagtaag ttgctttttt acgatcaatg atctcataag cagcattacg gactttatca
      541 gcaatcacat ctaattcatc ttgggcaata cgatggtctt tttctacaat ttcaaacact
      601 ggtttaccac cgacagttgt atgtgaccat gcagcaactt ctgaatcacc atgttcacct
      661 aagatatagc cgtggacgct gcgtgggtca acttttaatt tcaatgcgat ttctttacgg
      721 aaacgagttg tatccaatgt tgttccagta ccgataacac gtgatgttgg cagaccagat
      781 tcgttccaag cgatatatgt caaaatatct actgggtttg aagcaacaac gataataccg
      841 tcgaatcctg atcccatgat ttcttttacg atttggcgca tgattgacgc atttgtttta
      901 actaaatcta aacgagattg accaggtttt tggttgatac cagctgtgat aacaacgata
      961 tcagcatctt tacattcttc atagccacct gaccatacag ccacattttc atcgccccaa
     1021 gccataccat ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt cacatcgatc
     1081 aatactaatt cattagagat tccttggttg atcattgcat aagcgatact tgttccaaca
     1141 aaacctgtac cgacgattac tactttacga cttgtttttt tcatgaataa acagctcctt
     1201 ttcataacta tttgttactt tcatgaaaag tatagtcttg tttattcatt ttatcaacat
     1261 gttaattata atagttgtta ttataatttt tgatggaaac gaattcctga atacgcataa
     1321 cagataaatg acaaatgttt acacctatat cccctgataa tacaaccaaa agtttagaac
     1381 tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata agacagcaat cgcacctaaa
     1441 acaattttcc ttcatatgct ttagtgaaag attttcttct taatgtttat ttctccattc
     1501 attgggagta catgttgaat acttcttgaa taatttgaat aaagaagtcg tttgtttata
     1561 tccgatacga ttagcgattt cttccaacga aaaactcgtt tcttgaatca gttccaacgc
     1621 aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa gcatcaaaat acattttcaa
     1681 tttattacta agatagaccg gattgtagcc aaaatgagct gctgtttctt ttaacgtaca
     1741 agaagtaaaa tgcttatcga tataatctaa tactctaagc atctcatttt ctgcgcatcc
     1801 atctgctgat gaaaagcttt gttgttctaa taaatgagtc aattcgatca gtaatagtga
     1861 tgtatagaga tttaacgaac ggatcttatg actgtatgta tcaaaataaa tcaacagcat
     1921 attttcaatc aaatgcgtca aacgacttgt ttttccgccg cgaaaatgaa tgaattgtgt
     1981 atgttcccct ttttcttttg atgcatttat caaaaaagaa gaaagtagat tatttgtgct
     2041 tgccaaatga ttcaatatct ctgtagataa ggtttcttct tttaataaga tgttgattaa
     2101 aatatcctct tgccctgtgt attcgattct ttgagtagct tctttatcaa tgatcaccac
     2161 gtcactttct tccatctcta tcctttctcc attaataatt tgtaaacatg taccgctata
     2221 catataattg atttcaacaa aatcatgtgt atgctcggga acttccgcat atcgacggtg
     2281 tttactgata tgaatatttc cctgttgaaa tagatcccct cggtggattt ttggcggtgt
     2341 ctgaagatgg taagctaagt ttaaatcatt gatattcatt ccttgttcac gctgcagagt
     2401 ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc agctagttcc aattgactcc
     2461 ctcctttttg taagcggttt ctttcgctta ttagttcacg ctgtactgct attatatgca
     2521 aagctatttt tggtttcata aagactttct atggttggga catgaacttc ctgtgattcc
     2581 gctattgaga gaataaccac ttcatactat atttttgata agcgcttaca aaaatgaaag
     2641 gaacatgctt atgaataaaa agatgatgaa attttctctg ctgtcagtct ctcttatttt
     2701 gacttctgct ggtgcagtca cgtctgcaat tcccagtatg tcagctactt atgccacgcg
     2761 ttctttatct gctgttgaat tattaacgac tgctccctct tttatggtca ctatttttgt
     2821 tttgattagt agttttattg ctaggaaaat cggacaaaaa caaactgtga tcatcggatt
     2881 agtgattgca tcgatttgtg gtatcctccc cctcttctca caaagttaca gtattgtctt
     2941 agcttcaaga gctggactag gtattggctt tggattgatc aattctttgg cagtcagcat
     3001 gattgctgcc ttttatcacg gagatgaacg agcctctatg attggttttc aaagtgcatt
     3061 tcaaggattt ggtgcagctt taatgacatt tgttgcagga cagctagtac agttcggttg
     3121 gcaatatacc tttttagtct acgccatcag cttacctatt cttgtcttgt ttgttttgtt
     3181 tgttcctaca cctgaaacaa ctgaacttca atcgcatggc acagccccaa caagacaagt
     3241 aatcaaaacg aatcctaaaa tgattagtta tggtattttt ctcttgctgg tcatcgtggt
     3301 atataatgct gtatcgatta aattagccac agtcctgacg acttattctc taggaaacga
     3361 aactaatgca gcgaatatct taacaatcat gcaattggca agtatggctg ccggagtttg
     3421 ttttggctgg cttcagacaa aagcacatcg ctatatgctt acactttctt tactactgat
     3481 gggaatcggc tttgtattga ttgccactgt gataaacctg tatgtgattg ggtttggtgc
     3541 gatcctaaca ggaatttctt tttcgttatt cattccttac ttattcaacc aagtaagtat
     3601 ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc ttattggttg gcgctaattt
     3661 aggtactttc attgcaccat atggtttgct ctttctctct tttgcttctc ttgggagtaa
     3721 aacacttgct gttttcgtca atggcagtat cttgctgttg attagcgcgc ttattagcct
     3781 tctagtagta cacaggacga atagaaagac agtgataaga gtagatgaag aaataaaaga
     3841 acaattgaca acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg aaaaacataa
     3901 agatagatgg ctttggtatc ctggagactt tgagatccgc catggactgc tacaaaattt
     3961 ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg tacatggatg attgccatcg
     4021 gaacgtcaag tttaagagat attactttct tgatcaacct tccatgttta aagtgacgat
     4081 ccaaggagtt gggtatgtag aaattaatgg acaaaaacat ccatgtggaa aatggctgac
     4141 ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga catacatcag ggctaccagc
     4201 gatgtttatc gagggagacg aagtaaaatc agatattggc tggacagcaa gtaattttat
     4261 tgaagaatat ccagctggct ggagtccgct ttatgtcgat atcgcaaaag atccaaatca
     4321 aatctattat caaaaagaat gcgtccatcc agtcactgaa acggagattg gcggaggaat
     4381 cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc tggatgacca
     4441 tatcgaatct atcactctat gttatggaga gtccgatgta gaagctatgg atatcgatta
     4501 ttgctactat aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac gcgcttttcg
     4561 ctatttgttt ataccagagg tttctactgg aatggttcaa cttcaagttt ttcatgaatt
     4621 tttatctctt tcgaaacaag ctgctttttc aagcgattct tctttactga atcagatctg
     4681 gtcagtatca gaagaagcct tcaagttatg tagtgatttg ttttttatcg acggtatcaa
     4741 acgagatcgc tggatttggt ctggagatgc gtatcaatca ttcatgatca atcaatatct
     4801 tttttttgac gaggaaatca ataaacgaac gatattagca ttaagaggac aaaatgaaat
     4861 caagcagcat atgaatacta tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa
     4921 ccactatatg atgacgaaag actatgaatt tttacgcatc gtctacccta agatgcaatc
     4981 attaatgaga tacttgatgg agcaaacgaa tgaattaggg tttatttatg ggagagcaaa
     5041 agattggata tttattgatt ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca
     5101 agtcttatta ctgaaagcct atcattccat gatcacttgc ggagatatct tagctgaaac
     5161 atatggagag aacgaaaatt atcatactca tctctatcaa gaaaaatatc aactattgaa
     5221 acaaaaccta tttgactact tttgggatga agaacttggc gcttttatcg attgctatga
     5281 atctgggaga agaaatgtga cgcgtcatgc gaatattttc gctgttttat tcgattttgc
     5341 taacaaagac caacagcaat cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca
     5401 gattactact ccatatttca aattctatga acaagatgct ttatgcaaac tcggacagac
     5461 gaatatcgtc taccaaacga ttcttgatta ttggggaggg atgttatctc atgatgcggt
     5521 aactttctgg gaagaatacg accctactca aacaggaaat gaaaagtaca gtatgtatgg
     5581 agatccttat ggaaaaagct tatgccatgc atggggagca agcccaatct atcttttagg
     5641 acgctatttt atcggtttgt atccaacaaa gccaggttat gaagaattta tgatacaacc
     5701 taatctagat tcatttgaaa cactgaattg tacgctacct atcaaagatg gaaagatttc
     5761 gcttgaaaaa acaaaagata agatcgtgat tacttctgat agaacaggcg gaacagtgaa
     5821 aatgaatggt aaaagttatc ctcttctagc aaaacagcca cttgtcttaa caagctattc
     5881 aaaaataaat gcataaaaca aaaaactgct tgcgacatat cagaagactt taaatcttct
     5941 tctgatattg tccaagcaat ttttattttg aagtttgctt aattctgttc tttagaaaca
     6001 atgtttgttc tttttccggc aaaaagaaag ctagataata cgacaagtaa aaagaaagcg
     6061 atgattccaa ctgtcaaggc tatttgaccg tattctccta gtttcccaag tacaagtcct
     6121 ggtactagat aatcagtatc ggcaaatgta gaagaagcga ttttaagatc tcctaatact
     6181 ggaaggataa ataatggcag ccaactaatc aataagctat tgaaagcaga acctaatacc
     6241 gcccctcttc taccgccagt cgagttgccg aagacaccag aagcagctcc gcagaagaaa
     6301 tgtcctacta ccccaggaat gataactgta gtttgcaagc ctatcatagc aaacatggat
     6361 agtgttccta caaagaagct aacaaaaaag ccaattaata cagcattagg tgcatatgga
     6421 aaaacgatag gaacatctaa tgctggttta gaatcgggta cgagtttttt agcaatccct
     6481 tggaatgctg gaacgatttc tgccaacacc attcgaaccc cttgaaggac gataacaaac
     6541 ccagctgtga atgttccagc ttggatcagt gcaaagatta cagcgttgtc tccattgctt
     6601 agttctcttt caacaaatcc aggaccagct aacaaagcaa gtgcgacata tacgatcgac
     6661 atcaagagca tgatcgatac agtagaatct cgcaaaaagc tgaaactttt aggaatcttt
     6721 atgtcctctg tagaacggtc tttgtttcca aaaaactttc cgatccatcc actagaagca
     6781 taaccgatat ttccggtatg ccctaacgca acttgattgt ttccagtgat gttcttcata
     6841 aacggttgac aaagagcagg cgtaatagtt aatagtgttc cttcgaagac cccgcctaat
     6901 aagatcatcc acaaatttgc agtaaacccc gcgcttacta aaatgactgc tgttagacaa
     6961 gaaacataaa gcattgcttg acctgtaaga aaaatgaatt tatatcttgt gaatctagca
     7021 agtaaaatat tcacgatcat ccctactagc atgatcaaag cagtaggtgt accgtattgt
     7081 tgcaaagcaa tcgccactac tgcttcattg ctaggaacta ctccttgagt atgcagagct
     7141 gtttggaaca tcgaagcaaa aggggttagt gaattactga ttaaaccagc accgccgatc
     7201 agtactagga atccgacaaa tgttttgata gttccttgga tcacttctga aaatttcttc
     7261 ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga ggatggaggg ctgactgacg
     7321 attgacacaa acaaattcaa caatttatcc attgatagtt cctcccttga atcattttta
     7381 ttcttttgac aagatcttcg ttactttttc ttttacttct tcgctgtcaa tgatcgattg
     7441 caaaggtacg agcttttcat caggtataga agttagcgta tctttcaaag aatgctcaat
     7501 gaagaaataa tcagccatat cgctagatac acttccaaca tccgtatgac ttacttcaaa
     7561 ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg atgttcatct ctaccataaa
     7621 acttgttcct aatccacttt gacatactgc aaaaattttc atatcattcg tctccttttc
     7681 ttatgatcgc taatatttct tcttttgttg atgcgtgtaa taaatcttga agattttgtt
     7741 tattagaaag tagctttgtt aaatttgcca aagcccgaag atgtgcctca ttatcgatag
     7801 cagctaaaca gatgaacaaa ctaatgggat gggctggatt atcaagcaga tccacaggtt
     7861 gttgaagttt cagcaaagac atccctactt cgtttacgcc atcttctgga cgagcatgag
     7921 gtaatgcgat gtgatcgcca atatggataa acgcaccata ctgctccacg cgctcgatca
     7981 tagctttgat ataactttct tctattttat tttctgataa tagcggctgt gcggcaaatg
     8041 tgattgcttc ttgccagttt tttggttgat ccgataattg gatcatttct tctgttaata
     8101 attcagatag cataggacgt tgatcctcct tcttttctaa aagtttgttc attttacgat
     8161 ttaatacttg atagagcttt tcttcgtcta cgccttcttt taattcgata taaggcagta
     8221 gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc tggtatcagc caatctcttt
     8281 gtacttgatt gatcagctga tttttttcca agtcagtcat gattggatgc agaacataga
     8341 gagacttttt cttatcagta agtgtgagag gtacagtaga aaaaataata tcgtaagaag
     8401 tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag atcatcggga
     8461 ataattgctg aagttcactt tttaagatca atgaagagct aatgccactt ggacagacga
     8521 tcacggctct tatttttcta tttctttctt ctgcgtccgc ttttctgatc tctccgccaa
     8581 atagtatagt gaaaaaacca atctcttctt cagggatgct cttccctatt cgtctttcta
     8641 acggaagtaa tgcttttctt gtcatttcgt agatggaagc atattgatgc ttaatttgat
     8701 cgatcatcac attaggtaaa taaaaagaat aggaaatacg aaaataggca gggaccaagt
     8761 gataaaagac attcatcaat aattcacgaa acgaagcaaa ttctacggcc atcaggaatt
     8821 ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga
     8881 catctccttc aacgatcgat gctaagcaaa gagagaaaaa tataatctcc gaattttgat
     8941 gttcggaata gccaaggcca gtcaatactt gtcgactaat gtcagtgact gcaagagaag
     9001 ccaacagttc agcatctttt accagttcca tggatgtttt tccaattcga atggttgtca
     9061 aactcaaata ataagctaat tcatccattc gatttggtgc gaatcgaaga tctgatagtg
     9121 aaatcgtttt atctagcaaa tctctaactt tggcgtaata atcaatggaa agggttgcgg
     9181 ttaaatacgt gagcccaaat cttccagcaa gtgactgcat caaacgagca gtgcgatttt
     9241 ttgcttctct tcttaatatt gcttctgacc ctagcagcct aaaaccagtt ttcctagtat
     9301 acgacagctg acaatcttcc acttctaatt catttcttat ctttttgata tctgacagga
     9361 ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag
     9421 tcgaataaac catcaaataa agaagatctc ttcgctcttg ttcggtatac acagcttcaa
     9481 atcgagtaat atccaaaaat ttttctgccc agtcgattgc tcggaatttc tttatacgga
     9541 tacgggtatc atccacatat tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat
     9601 ttacttcgat gatctctgct gctttttcta gactgatttc tcttggtgtt ttcaggagat
     9661 aactaatttt gtagttcaca gcgctgttca cctcctactt atattctagt atggtagcgc
     9721 ttttaaatcg aggggtgttt ggtacaactt acagttggat tttttttacg actgtaccga
     9781 acgatttcta tcgaatggat acagaaaaaa agcaggtgac caaatgtctc ctgcttttat
     9841 gaattgattt cgtccgatca atgatgatca tgtttatttt ttgaggtcag ttcttcaaat
     9901 ttttctttga catcttcaac cgctttttcg atatcttttt caaagtgatt gggctcatta
     9961 ggctttgttt cctcttctac agaatttgaa ccagataaag ctgtttcttt attttctgtt
    10021 tcaccagagt ttgtgccagc taatgattct tctttgtctt ctgtttcacc agaattagtt
    10081 cctgctaaag ccacttcttc ttctgtttta ggggccattg aacctcttaa tcgtttgtgt
    10141 ttttcaccgt cataatttgt catgttggtt cctcctagta gttctatact ttttcatcgt
    10201 acaataagac agtttatgcg tcaaaaaaca aacccgattc ttacaaatat gtcatatttt
    10261 tccttctgat tcttgaatgc ttttgattcc ttcttccaaa attttcattt gtaagattgt
    10321 ttcttcatct ttgatgactt tttctgcgcc attaactaat gtctcgtaaa tcccttcgta
    10381 gacacggctg taatcaccaa cctcggaaat tactttttct tcatgataac tcccttgttc
    10441 atctatatag gtcaatacac cataatgttc gggcaaatcg ataccaaagt cttcatgatc
    10501 aggcatgtaa aacattttca gatgttcttc ttgtcgatct tttgtttgtt tcacgaacat
    10561 cccttttttt ccgtaaacga cgaaacttgg acgttctttg atgcggaaat aactggattt
    10621 tatcgaaatt ttcatatttc catagaaaaa atccagatcg aagtaatcat tcattcgtcc
    10681 tttccctaaa agttgacgta catcatagct gatatcatct ggttcgccga aataagaaag
    10741 aacttgatct actgtatgac aagcatgtcc atataaatag ctgttcgcag tagaaaattc
    10801 atgcgtattt tctggtactt ccggtctgaa ataatcataa tgcatttcta cttctaaaat
    10861 atcccctaat tttccgcttt cgattacttt ttggactgtc aagaagtcag aatcgaaccg
    10921 tctgttttgg taacattgga taaatagacc tttttctttt gctaatgcaa ataactcttc
    10981 tgcttccttt gacgtttctg caaaaggttt ctctactagt acattcttac catgttcgag
    11041 tgccattttt ccatattcat aatgaaattg actaggtgta gtgatgacaa ctaattggat
    11101 ctcaggatca tcccaaagat cagacagctg atcagtatat tcgatgtctt cgaatgtttt
    11161 ccacactggt tcagatgggg aaaaaatttt tttgatattg attttgttcg taagttttct
    11221 tgaaaaaggc aaatgatagc gatttgtact ctttccattg ccaagataag cgattgtaag
    11281 catgagtggt gcctcctttt attgatgttt tcagtatatc ctagaaaagc gcagaaagta
    11341 tttataatag ctattctggt aaaaaagttc agaaaaacac ttgttatcat tcggtttggt
    11401 gaaattaatg ttcaaagtaa ggataaaaat caaaaagata gtagcggaac caagtgttta
    11461 tcagtaagat agaatttgga ggtcagatca tgcttattga agaaaaacta acaaaacaag
    11521 agttgtttac cacgacagag aaacggattg tcgattacat ccgtaggaat atcgaagctg
    11581 cagtctacat gacgattgaa gaattagcaa aagctacgta tacttcccat tcagctatca
    11641 ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga attccgtttc gagctggggc
    11701 gggaagttca tcaattgatt gctcagttca atcaaacgat tgatcctaat tttccttttt
    11761 caagtgaaga tagcactcag caaattgcaa agaaaatggc ggatttatcg atccagtcaa
    11821 taaaaaaagc acaattgcag attgaacatg aagatttaga tcaaatcggt aaagtattga
    11881 acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg gcaaggaaat
    11941 tccagaataa aatggtcaag ttgaataagt ttttgatcat ggctgaagaa tacagtgatt
    12001 cttcttggaa tgcagccaat ttgactagcg aggattgcgc catttttatt agctacagcg
    12061 gtcgcatcca tcattatgaa cgaataatga cctatttgaa gcatgtagag gcccctacct
    12121 tgctgattac cggaaatcag cattcagaaa tggccaaaca ggctgatatg tgtcttgtta
    12181 tatcgcagga ggaatatgat tttgctaaag tagcgacttt ctcctctcaa attgccttcg
    12241 attacgtatt aaatacgtta ttctcagtga tatattctca aaattttgaa gagaatatcc
    12301 gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa tatcggaaaa
    12361 cttaagaaaa gatcctcatt tagtaataag aaagatagtg tttgataggc tctctttgat
    12421 atcatcataa aaacaggctg aagttttctt tttcaaacct cagcctgttt gtttacggaa
    12481 cgattcgaat agatatatta tagaaatgag gaatagctaa cttctgtttc ttattttgaa
    12541 cgattgacga aaaagatata taagaaggag gaaaatcagt atacatgcag ctaacaatcc
    12601 atacaggaaa aatcgtttgt tctcggatac tttctgtact tctttttgaa cttcttttgc
    12661 atgataagga acccggtgcc ctctgacaag taagcgatga ctgttgacca tataaggtgt
    12721 acaagttagg agtgtcacga aatcctgccc tttttcgatt ctcagatctt ttgtatcagt
    12781 cggttctacc gtcttgatct gatcgacttg ataagcgagt gtccgtccat tgatttcgat
    12841 atagaaagaa tcacctaaag tcagtttcgg taagtctgtg aacaactttg cttgaggcag
    12901 tcctcgatgg ctggaaataa cggcatgtgt attttctccc cccgtaggat aagacgttcc
    12961 ttccaataat gaggcacctt tttctagcag cacagtattc gtttcatcaa aaattggcaa
    13021 atggacattg atctcaggga tcgtcaaaat accgatcgta tgtttctcaa aataggttct
    13081 atcctttttc gcaggttctt ttttcttagt aaagggatct gcacctggat ttccaccttc
    13141 ttttgctaat tgctggtttt tctttgtcat gttttcttgg attttagcca tgacttcttc
    13201 attttctttg accgcttgtt gctgataatg agagatgatc tgctgatcca gataattatt
    13261 cagcgcgtcg ctaacaaatg gataagctag tatccctatt cctgagagaa gcaagaaaag
    13321 catcagcaga tctagaagtt tccttttctt tggtttcatt tgtgttctcc ttgtgatgac
    13381 tgtttgataa taaaagaaaa aggagcattt gcgtaatccg ctgctccttt ttccaattct
    13441 tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca gcaatcagta
    13501 gaaggactac tcctgcaccg atatacacat agattccctt accgcctgtt gaaggaagtg
    13561 tacctttgtg tttatttggt attttttcag gagaaatcaa ctgtcctgct gttacataag
    13621 attgttcatc gatagtaaat gctacacggt ttgttaatgg aacatatttt tctggcgctt
    13681 tcgtttcttc cagatagtac gtgccatatt ttagacctgt cacatcgatt aaaccgttac
    13741 ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac ccagctgacg gcttttgtgg
    13801 atgggtcgat cgataaatat ttcgctgtgt cactatcttg atcacgaacg acgaattctg
    13861 ctccagcaag tgtttggtct gatgtaacgt caccatctac tttaacaaat cgtttgcccc
    13921 cagtaacgac atcgactgac ggtggtgttt gatcatttgt atgcccgtta tcgacatttg
    13981 cttggttaga aaatcctttg gttggatctg ctttttcgtt caaatgcata tagtaaacga
    14041 atttcaatgt accgccagga gttaatgaag gaatataatt cggatcaact gaaaccgtga
    14101 atccgtctgt ttgctcagtg acagaataat ttactgggtc gatttcttta tttccatcat
    14161 ataaggcata agcatacgtt cctgaagaat cattatcaaa tgttaaagca gcgtcatgag
    14221 tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca gcgataccta
    14281 atgggatatt gacagaaatc tcatattgaa ttttttcacc gattgcgaca tcttttccat
    14341 tcaattgagg tgttgtttta tcaactttag acgtatcatt tttgatggtc ttttctactg
    14401 gtgtttggct atttgccagg atctcaaaag gcgtgattgt ttgcttttca atcatttccg
    14461 cattatcagg agcttttact tcttctaaat tatattttcc aacttctaaa tgattaacga
    14521 tcaactggcc tttttcaata gatgcctcgg caaagtcatt gttgccgatg tcataagaat
    14581 gaccagtaat gaaatgtttg gctttggttt gatcagttgt ccaagtgtac aatccatctg
    14641 tgacactttg gatgtatttt ttgacgcttg gtgttccttc ttctttagaa ataataaatt
    14701 ctgctccatt tagtgcttcg ttttcggcag taccgatttt tgtaactttc aacgttccat
    14761 cattaccgac tgtattttta gggtagagat ggatagtatc tagttcttct gtcccgtatt
    14821 tataagagcc atctgcttgt ttgatcatct catatacagg gaaagctaaa accatgtttg
    14881 cggcagctga cactccgtct tttggttctt ctttgatcgt gtagactgca tctttcccat
    14941 tttgtttttt aggtaaagat aaagtgacat ttccatcagc atctgtcgtt cctgaagcaa
    15001 ctggtgtacc aggagtcaaa gactggactg cttgttttgc tgcatccacg gacgctcctt
    15061 tatctcgttg cgcataaaat tcttgagtga cgttataaac tgaaaatgaa atatcggcta
    15121 atccttggta ttgatcgaat tcgctcattt ctttcccgct gttttggatt aaaggatcgg
    15181 gtaaatcagt cattttcttt ttatgaagga tgacttgagc tgctgtctcc tctgcaaaca
    15241 ctttttttgc gccgaagctg tttgtgagta atggtaaaat aaggaaaagg actcctaaca
    15301 taacgtttat ttttttatga tttttcattt tttcactcct gttctttttt tgataaacaa
    15361 ccagattgca aagcttgcga ataccatacc aacaacgcct aaaagtgtat ttttcgtttc
    15421 gtttgttttt ggaagaaatg gacggctatt ttctttttta ttgactactt tcaatatact
    15481 gtccgcctct ttatcctcag aaagaaaagt tgaaatggta aacggaattt ctgtttcact
    15541 taaaatataa ccagaaggtg cttcgatctc ttttaatcga taacttcctg ttttcaaccc
    15601 ggaaattgaa aaagcgccat ttttatcgga aatcaggtga agcgcatctg attcgttctt
    15661 tgtccacttg tatccgttgg ctgtttgctg gaggtaattt cctgctttgt ttttaataag
    15721 gaatactgcg ccagctaatc gtttatctgg tttatttgtc tcgattttga caaattgctt
    15781 actccctgta tatacagaga ctttctttgt actgacacca aaaccaaaat ccagtgttcc
    15841 gttgttgttg atttccttgt tcgcctgtgc tgtactgctt agctgcatct gataagaaat
    15901 ggtcagtttt tttccagcat atttttccaa cgaagggatc gaaaataaaa catcaaatcc
    15961 gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt tctccattcg atgaaatcgt
    16021 taaactatta ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt cgaacttttg
    16081 atagtcaagg atatttacag gaattgtcgt atgcaaggaa taagtgattt tttcaccaat
    16141 cgtaaaatca tttctaggct cttctaatac cttctcaaaa ggtggatcag ggtagtcgtt
    16201 ttcttcattt ttaggataca aatggatagt tgaaagtttt tgattgtttt gtccatgtac
    16261 aggaagaaca actaccatat tctctgcctt ttctttgacg acttctggta ctttggattc
    16321 aatgaaaaga tacgctttat ctcttttttc ttgatcttta gcggccagag aaaaagaagc
    16381 gataccatct tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca taccggaaga
    16441 cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc gttttgccct tttcccgtag
    16501 atggtaaaaa gaatctgtga catcataaac ttggaatgtg acaccattta atcctcgata
    16561 cgtttgtaat aaagcttttt cttgtccgtc atttggatga tttttcggca gttgcccgtt
    16621 tgggaaaagc aatttgtgta aagtaatctg aaccatttct gtttctgttt ctgttgctac
    16681 ctgagtaaaa gctggtttaa atagtaacaa gaagagacac atactaagcc aaccaagttt
    16741 tttcatattt agctgccccc tctttctttt ttcaaaaaaa gataccaaat agaaaacgca
    16801 caaccaatca tccctactag atagattcct aaacggccaa ttcctcccgt ttcaggtaat
    16861 ggtacttttg cctgattcgt gatgtctaaa gaaatctggt tgtttttggc tcctggtgac
    16921 agaacagatt catgatcttg tccatccact tgaattgacc catcttcgtg tataactata
    16981 gtaactggct cttttagacc ttggtatcct tctggtgtaa aagtttcggt cagcgtataa
    17041 gttccaggtt ttaaattttc gaatagaaag gtatctgttt cttgtccatc ttttggcgat
    17101 tctaagtcca tttctggtcc ctgcagtctg aattttgctc cttttaatgt ctgtccgttg
    17161 tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc gattttgatg agtgagcgtc
    17221 catacattat ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc atatttatcg
    17281 atttggaatt cataatttcc cggcgaagta tcatatccgg caggaccagc tgtttcaacg
    17341 actcgatatt taccaggtgt gagtgaatca aaactggcaa gacctttttc attcgttgtt
    17401 tggctgtcaa ttggctgata agtaccattt gcttcttttt tctgtaggga aaatgctgct
    17461 cctttaagat ttatctcttt gtccgtccct tgcatcgcat acttacgtac tgctacagga
    17521 acttcaacaa aaggattttc aatagtcaac tggatcgtat catcggaagt agtgactgtt
    17581 tttccatcaa tggttaccgt accatcagaa gcgatcttga tttcccaagt agtctttttg
    17641 cttaatccat gcccttctgg agcttttgtt tctgtcagcg tataggtcat ttctttttgc
    17701 aatttgacat tttctggaag agaataggtt ccgtcgccat gatctgttag taaagtatca
    17761 atagaatctc ctgttaattt gaaaacagcg ccaataagat ccttttcacc tgtagaggaa
    17821 tttttcgtta ttttcaagtt taacggtgtg aattgtttag tatttttcca tgtcattgca
    17881 tctatttgtt gagaatcgta tcctggtaca ggtaattctt tgattgtttg gtaactgaat
    17941 gcttgacctt gattattgta ttgaggtagt gataatatct cttgataact ttctccgcta
    18001 tttgcagata atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc tttagctggt
    18061 ttaatgattc gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg ttcccgttga
    18121 atctcaaaag taacttgatc tggacgatca gctagattgt tgtcaaattc ttcccataac
    18181 tttttgatgt gaagactgac tccgggagct ttagcagatg gtataccgaa ttcagctaat
    18241 tcattggtat caatgtttgg ctggaaagtt gtcctgccgt tcatttgata ccagaaattt
    18301 ggatggaagt cctcattttc tgtttggatt ctcacttggt aatggatttg gatttcttgg
    18361 tcttttccta aatagatctg attgctcttg atggtatttc cttctatgga aatagatgga
    18421 gtgaccgttg taggacttgt ccccacactc ttgactgaaa gtgtaccagg ctgataaatg
    18481 aatggttctg cgattggatc atttatttgt ccattgctta cagttgctga gatctgtaca
    18541 gcttttttgg ctaggtattc agagatatct gttgcatgat cagctgattc atagtaaaga
    18601 tcgccttttt catctgatga aaccatttgt cgcatacgag actctacttc tgcttttgag
    18661 agaccagctg ccggatcgct ttgaagttgg atgccaagac catgtatttc gattcctcgt
    18721 tctttgagtg ccatcgcttc tccgatggtt gcgataaacg tactgtcgat tcttctggat
    18781 agattgtttt ggtcaggtgc atcatagatt cttgagatta gagaagtatt tcccggccga
    18841 tcttgcgtat tagaaaactg agttccgtaa taattgctgc ttgattgtgc gtgtacccgc
    18901 tgtactttat aggaaaatgt tggtacacca tccgtcagca aaacgatcac ttttttatgt
    18961 ccatttggaa cggatagcat gcttcctgca tctcttagtg ctttttgtgt aaaagtacca
    19021 ccatttgtcc gtgaaggtgt aatggatttt acttgatttt tcactgaatc aaatgaaccc
    19081 atctgtactg caccgttact gtagctatat ccttcgcttg agtagccgac atatcccata
    19141 ttgattttgt ctgtgatacc gctatctgct aaagtatcga caaaacgatc gacaccaatc
    19201 tttacttcac cgatccgatt attgtcgttc atacttcctg accagtctac gaccaatacg
    19261 agatcaagag gcgtgatatc tttttgtaca tttccacgag cattcaaata aacatcaaac
    19321 aatccgggtg tacttgtttc ctttgcatac tttcgtatcg catagtctgc ttgattaccg
    19381 gttcctccat attcgatata cgaatgggtc cgatcattag gatctccatt ccaagaattt
    19441 ataccatccc attgatttgt tccgttttta ttgccttgat gatcaagaac atttgtattt
    19501 cctgtaggtt gccaactggc tttaggataa gtgccctgat catctgttgt atattctgta
    19561 ggaatcgttt gattagatgg cacactcaca gctggcaagg ataatgtagg caatgtaaat
    19621 gggctgacag aattttcgtt ttcttttgat gattcttctt ctatttcgtc tggctttggt
    19681 actgacacat ctgtatcttt cttattttct gtctcatcaa taatgtcata ttctttatta
    19741 tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg aaaactcaga
    19801 tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct atttacctga
    19861 taagcatagc catctccttc ttttctttcc acatcactta agtaattttc gactagacca
    19921 atttcttttt ctatcttttc agcactggtg ttattcgcat ttagcttgat gttcagtgct
    19981 ttttttccag actgatccga aatgacttca gctgaaattg tttctaaaac tgttggatca
    20041 tctgtagtgt ctgctattgc agataatggc acaaagtttg ataaactcaa tattatcagc
    20101 atgaaaataa ctttcagttt cttccctgtg gtcgtcattt ttcccctcct ttttcttttt
    20161 ttctgttttg acgtgaatag tgaaaattat taaaataaac aggcgttaca taattttatt
    20221 tattttaata tgataattat tatacgaagg gcgctaaagg gaatcacacc aattttcata
    20281 ccaattggtt tcatttatga agttgattcc ttcgattaat gagcgtttca ttgaaagaaa
    20341 agggttgagg gagaaaaaca tatgatcagg ttgttggaag gaaactatag aaagatggaa
    20401 aaaatcattc gaattctgtc catttctgac aagtggatga ctaaaaaaga acttgccaaa
    20461 cgaattggta gttcagaatc tacatttgta aggtatatcg aagagatcaa aatccgctgg
    20521 ggaacggatg taacgattca atcttcacaa aaattggggt atcgtcttga acatttgaat
    20581 gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa cagtcgttta
    20641 ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt attattgtga aaaaatttcc
    20701 atcagccggt cttcttttac acgtaagttg aaacaatgca atcaaatttt agaaagctat
    20761 ttattaaaag tgatcgttga ccaaggtttt cagttaacta gccttaaaag tgagctccag
    20821 ttgcgtatct ttgttacctt tttcttttta agttactacg gaagacgcac gttgccattt
    20881 ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga ataattgtca gatgaatttg
    20941 tttcaggaag aaagcccata tgagcaatct ttctttatta tgtattttgt tgtgcatctc
    21001 atgagagaat cccaaggaac tccatttgaa tggaaagctg gagactctta tgtacagttg
    21061 ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa attgaagtac
    21121 agcagctata taaaagtagt gaactatttt gtcaatacga ttttttcaga attttcggag
    21181 aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata tgtcctactg ttcgttttca
    21241 tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat
    21301 tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt
    21361 gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa
    21421 ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg
    21481 atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc
    21541 agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct
    21601 gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat
    21661 cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat
    21721 atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag
    21781 ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat
    21841 acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa
    21901 gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat
    21961 tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa
    22021 atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc
    22081 tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata
    22141 gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa
    22201 atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct
    22261 tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg
    22321 cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca
    22381 aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa
    22441 agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat
    22501 tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc
    22561 gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt
    22621 tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat
    22681 ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag
    22741 caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg
    22801 actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag
    22861 taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc
    22921 tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct
    22981 cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg
    23041 aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc
    23101 cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa
    23161 atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc
    23221 tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac
    23281 aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc
    23341 gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac
    23401 cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg
    23461 tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc
    23521 gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg
    23581 ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt
    23641 cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg
    23701 ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga
    23761 cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa
    23821 aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag
    23881 attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg
    23941 taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt
    24001 gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc
    24061 tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc
    24121 ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc
    24181 tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg
    24241 taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata
    24301 gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca
    24361 cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac
    24421 atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac
    24481 caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat
    24541 ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta
    24601 gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga
    24661 tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc
    24721 cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag
    24781 gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag
    24841 cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca
    24901 gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa
    24961 aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc
    25021 agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt
    25081 atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct
    25141 tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga
    25201 aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt
    25261 ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca
    25321 ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa
    25381 ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca
    25441 ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt
    25501 gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg
    25561 gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct
    25621 gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac
    25681 atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt
    25741 atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct
    25801 agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat
    25861 gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt
    25921 accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag
    25981 acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat
    26041 cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa
    26101 ccatttcaag agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca
    26161 ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat
    26221 gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa
    26281 gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg
    26341 atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg
    26401 cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc
    26461 aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa
    26521 tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa
    26581 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca
    26641 cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta
    26701 catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa
    26761 catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta
    26821 catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg
    26881 ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga
    26941 ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag
    27001 ttgtcacatc atgtgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat
    27061 ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc
    27121 caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa
    27181 agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc
    27241 ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg
    27301 gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt
    27361 gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag
    27421 tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg
    27481 tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag
    27541 tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata
    27601 atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa
    27661 ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt attctatagg
    27721 agacgattgt tttctacaaa tatttagatt ggaatttgat taaaacaatt
//
LOCUS       DARDHD010000018        26134 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000018 DARDHD010000000
VERSION     DARDHD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.018"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..217)
                     /locus_tag="SDA06_000633"
     CDS             complement(20..217)
                     /locus_tag="SDA06_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG4227928.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(611..2338)
                     /locus_tag="SDA06_000634"
     CDS             complement(611..2338)
                     /locus_tag="SDA06_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG4227929.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2331..3524)
                     /locus_tag="SDA06_000635"
     CDS             complement(2331..3524)
                     /locus_tag="SDA06_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4227930.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3711..4355)
                     /locus_tag="SDA06_000636"
     CDS             complement(3711..4355)
                     /locus_tag="SDA06_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4227931.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4449..4583)
                     /locus_tag="SDA06_000637"
     CDS             complement(4449..4583)
                     /locus_tag="SDA06_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG4227932.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4585..6717)
                     /gene="feoB"
                     /locus_tag="SDA06_000638"
     CDS             complement(4585..6717)
                     /gene="feoB"
                     /locus_tag="SDA06_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG4227933.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6714..7187)
                     /locus_tag="SDA06_000639"
     CDS             complement(6714..7187)
                     /locus_tag="SDA06_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG4227934.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7588..7812
                     /gene="nrdH"
                     /locus_tag="SDA06_000640"
     CDS             7588..7812
                     /gene="nrdH"
                     /locus_tag="SDA06_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG4227935.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7971..10130
                     /gene="nrdE"
                     /locus_tag="SDA06_000641"
     CDS             7971..10130
                     /gene="nrdE"
                     /locus_tag="SDA06_000641"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG4227936.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10180..11145
                     /gene="nrdF"
                     /locus_tag="SDA06_000642"
     CDS             10180..11145
                     /gene="nrdF"
                     /locus_tag="SDA06_000642"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG4227937.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11234..12373)
                     /locus_tag="SDA06_000643"
     CDS             complement(11234..12373)
                     /locus_tag="SDA06_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4227938.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12682..13395)
                     /locus_tag="SDA06_000644"
     CDS             complement(12682..13395)
                     /locus_tag="SDA06_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG4227939.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13649..14350)
                     /gene="nagB"
                     /locus_tag="SDA06_000645"
     CDS             complement(13649..14350)
                     /gene="nagB"
                     /locus_tag="SDA06_000645"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG4227940.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14584..16116
                     /locus_tag="SDA06_000646"
     CDS             14584..16116
                     /locus_tag="SDA06_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4227941.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16307..17509)
                     /locus_tag="SDA06_000647"
     CDS             complement(16307..17509)
                     /locus_tag="SDA06_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4227942.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17591..18280)
                     /locus_tag="SDA06_000648"
     CDS             complement(17591..18280)
                     /locus_tag="SDA06_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4227943.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18386..18754)
                     /gene="rplL"
                     /locus_tag="SDA06_000649"
     CDS             complement(18386..18754)
                     /gene="rplL"
                     /locus_tag="SDA06_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG4227944.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18807..19310)
                     /locus_tag="SDA06_000650"
     CDS             complement(18807..19310)
                     /locus_tag="SDA06_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG4227945.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19514..19641)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19720..20409)
                     /gene="rplA"
                     /locus_tag="SDA06_000651"
     CDS             complement(19720..20409)
                     /gene="rplA"
                     /locus_tag="SDA06_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG4227946.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20513..20935)
                     /gene="rplK"
                     /locus_tag="SDA06_000652"
     CDS             complement(20513..20935)
                     /gene="rplK"
                     /locus_tag="SDA06_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG4227947.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21278..21946
                     /gene="sdaAB"
                     /locus_tag="SDA06_000653"
     CDS             21278..21946
                     /gene="sdaAB"
                     /locus_tag="SDA06_000653"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG4227948.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21959..22831
                     /gene="sdaAA"
                     /locus_tag="SDA06_000654"
     CDS             21959..22831
                     /gene="sdaAA"
                     /locus_tag="SDA06_000654"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG4227949.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22922..23941)
                     /locus_tag="SDA06_000655"
     CDS             complement(22922..23941)
                     /locus_tag="SDA06_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4227950.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24114..25019)
                     /locus_tag="SDA06_000656"
     CDS             complement(24114..25019)
                     /locus_tag="SDA06_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4227951.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25078..25623)
                     /gene="nusG"
                     /locus_tag="SDA06_000657"
     CDS             complement(25078..25623)
                     /gene="nusG"
                     /locus_tag="SDA06_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG4227952.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25724..25894)
                     /gene="secE"
                     /locus_tag="SDA06_000658"
     CDS             complement(25724..25894)
                     /gene="secE"
                     /locus_tag="SDA06_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG4227953.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25918..26070)
                     /gene="rpmG"
                     /locus_tag="SDA06_000659"
     CDS             complement(25918..26070)
                     /gene="rpmG"
                     /locus_tag="SDA06_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4227954.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
BASE COUNT         7824 a         5455 c         4633 g         8222 t
ORIGIN      
        1 cttagcattt tttttcattt tagcacttcc ctggaattgc tccacctaaa aaaccaccta
       61 tagacattcc tgcaatacaa gtagttgcct tggcccaatc gaccgtacat ttatttttag
      121 tgcaatacac tccatttcca taatattttc cactatgagt ggtaccccca tagataagtt
      181 gtgtctcttt aatagacaaa atttttaaat gtttcataat ctaatcaccc ctaaattaat
      241 gtttttagac tattattcta acattcatta aatttcaaaa aacaattcct tcttgaaacg
      301 tagcaaaata attcctgaat aagaatttaa tcacttttaa gtatataaac tatgtatatt
      361 ctttaatatt ttttgagaat gcacttcgta cataataaac tttaatcaca ttagtctttc
      421 atacctacga tcgatctctt tgactcgcac ccataaattc ataatagttt gtcggcgtct
      481 ttggacgttt cgaagatgag ctacgcattc tcttttttta tagacaaaaa agatagagac
      541 gaacatggag cttccaaatt tggatatttg atccaatttt ggaagctcca tttatttcgt
      601 gtctgtcttt ttattttttc cagctttcga ctaattcttg gttctctttg atccagtctt
      661 ttgctgcttg ctgtggatct tttccattgt tgatttccaa cataactttt tccatatctt
      721 tttctgacca atggaaattg tctaatactt tgtaagcttc tggctggtct tctttcaatc
      781 cttttcgaac catcgtatgg atctgctctt cgcttcccat tccgttttca ggatcttcta
      841 agtatttcag atcatatttg gcaaacatcc aatgaggtgt ccatcctgta actacaattg
      901 gttcgtgttt tttgatcgct tgacctagtg caacggtcat tgctcctgaa gaagatgtct
      961 ctactttcca gtctttcaag ttgtcatatt tttggatcgt gttttcagca gccgtaacta
     1021 caccagctcc tggctcgatt cctatgattt tcttacctgc ctgatcggtt aactcatcga
     1081 tcgaatttac atccatgtag gctggcactg ctaaaccaac ttttgctcct gtcaaattgg
     1141 cacctaggtc ttcgacttga tctttatatt gtgcatattg tgaaccatga gttttaggta
     1201 gccaagctga aaccatggca tctgactcgc catttgaaac agatttccac atgattgaat
     1261 tatctaatgg cgtcatcgtt acatcatagc ccatttgttt caatacttca ccgactacat
     1321 ttgttgaagc aacttctgta tcccattcaa cataagataa gttgatacgt tttgtttctt
     1381 tggcagatga aaaactccct ataccaatcg cacctaagat cactactact acagcaccaa
     1441 tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc tcctgcagct tttttattgt
     1501 ttggctggtt caaatgctgt gtaaaacggt caatgatgat tgctaagata actaatgcaa
     1561 ccccgttgac aaatccattc ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc
     1621 ctaagccggg tgcaccgatc atagaggcaa taacgaccat tgaaagtgac agcattgttg
     1681 tttgattgat ccctgccatg atcgtacttt tagctaaagg caattctagt ttgaatagtt
     1741 tttgacgact tgtgctcccg aatgaatcag aagcttctac taattcttta ggaacttgtc
     1801 gaatccctaa attcgtaaaa cgaacggttg gtggcagagc aaagatgact gaggcaaata
     1861 cccctggaac catcccgata ccgaaaaatg caactgctgg aatcaaataa acgaatcctg
     1921 gcattgtctg cataaagtca aggatcggtt tgataatact ttgggcttta ctgctttttg
     1981 ccatcaagat ccctaatggt accccaatga tgatcgagat cacactagat aaaagaacaa
     2041 gtgtgacagt attcatcaaa tcattccata gaccttggtt ataaataaac catagaccga
     2101 ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat aaagaaagct gctaccgtca
     2161 ataacaagat aaacaaagga gctggaatca ctaacagtaa atttgtaatc cccgacatca
     2221 ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt
     2281 ctgtgatatt ttctacccag tttgccactg gtaattgata gttcaataga ttattcatgt
     2341 tcactcacct cgcttgtttc tgctaatgct tcaatcacac tacctcggat gacgacacct
     2401 tttaattttc catcctctac aactgcaagt ggagtcggtg agtcataaat aatattgaaa
     2461 atatcactta ccaccatgtc tttcccaatc accgtcacat tttcatctag gaagtcgatc
     2521 aatggacgtt tttgcttcct agcttctaac gcttgatctg cagtgatgat ccctttcagg
     2581 tgtctctttc tgtcaacagc catcagcatg ctgacttctt cattacgcat ccttgtcaat
     2641 gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg caggtaccat gatgttttga
     2701 gccgtaagca ctttggaacg atcgacttct tccacaaatt cacgtacata atcatttgct
     2761 ggattggtca atatttcttc acctgttccg atctgcatga tttcgccgtc tttcatcaag
     2821 gcgattctgt ccccaatccg taaagcttca ttcaaatcat gggtaatgaa aataatcgtt
     2881 ttttgaacat tggcttgcag gtccagtaat tcgtcttgca tttctcgccg gatcagcgga
     2941 tcaagagccg agaacgcttc gtccatcagc aagatctcag gatcatttgc taaagctctg
     3001 gctaggccaa cccgttgttg catcccacca gataactgac taggatattg gtctttaaat
     3061 gaaagcaaac tagaattttc taatgctttt tctgcttttt cttgtcgttc ttctttaggt
     3121 acaccacgga cttctaaccc gtattctgta ttttccaaga tcgttcgatg tggaaacaga
     3181 ccgaagtttt ggaaaaccat attgatttta tgacgacgga cttctcgcaa ttcttctttg
     3241 cttaacttcg atacatcttc tccatcaata tagatactgc ccgaagttgg ctcaatcaga
     3301 cgattcaata gacggatcaa tgtggatttc ccactcccgg aaagccccat gatcacgaag
     3361 atttctcctt cttttacgtc aaaattcacg tcatacacac caaccgttgc accggttttc
     3421 tttaaaatct ccgttttcgg ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt
     3481 ttaccaaata tctttgttaa atgttttact tctactttta ccaaacaaat tctccttttt
     3541 tatgtaaatt catttttctt tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc
     3601 actttgtcaa aaatgaaaga aatttgcttg tccaaactga aaaaaacttg gctaaatctt
     3661 tgtaaataaa aggtttagcc aagttaatat ttttttaata tttcacacaa tcattctgca
     3721 agctgatgct ccgggtaaaa aaagtttgtc atacgctgta gtgtatattc atttccaata
     3781 aaatgaacga tgtcccctgc cgtaaattta gcataggggc caggtgaaac aagcagttct
     3841 tcattatgag aaattgcgat aactgtggcg ccagtcgcct gccagatatt caactcgcta
     3901 attgaacggt ccagatgcat tgctttttct gataaagata attcaaacgg aagtaaagga
     3961 ttcatcttat gtattcgctt cgtttgattg accagctgat ccaataattg agaaaaatga
     4021 ttcagctcat cttgttgctt ctccacactt gccatgattt ctgcttttgt atcttggatg
     4081 gattgtacat cttgatattg caataggaaa tcttttgctt tttccttcga agcaacaaat
     4141 gccccacttc catgccgtac ttccatgatt ccaagatcta ccagcacatt gatagccttg
     4201 cgggtagtct caggagaagc attgtacgta ctggcaagtg ttgagcgggc atggatcttc
     4261 tcacccactt tatatcggtt ttcaacgata cgttcggcta aatcaacagc aatctgctgg
     4321 tagcgcgggc ggacgatttt tttctttcca gacattttct ttcctctttc tttttttact
     4381 ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca
     4441 acgactgatc atcgcttgat ctgttccttt tttactgggc aagctgtttg acagtcatca
     4501 caattcttac cggattttaa acgagtatag acaattgcgc ctgcacttcc aaagattaac
     4561 acacttaata agaatgtcgc catcctattc ctctccttct aatgacaata tagtataaaa
     4621 ctggttcttg gattttggct gacggaacaa aacataaacg agtcctatca tcagtaaaat
     4681 agcaagatac gtccctaacg ctacctgtcc accctccaat aagtgtccca actgataaat
     4741 cacaaaacta actaaataag ctaaaccgca ttgatagcct atggcgatcc aagtccattt
     4801 cttggcattc atttcccgat ggatagcccc aattgccgca aaacaaggcg cacaaagtaa
     4861 attgaaggct aacaatgaat aagctgctgc gggagtatat gtgtgttgta gcacttgcca
     4921 gatttctctg ccattttcgg atacttccgc atgaccaaac aaaataccaa atgtgccaat
     4981 aatattttct ttagcgacca atcctgtaat cgctgcgact gttccttgcc agtttcccca
     5041 accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa aaggctaaga tactttggtc
     5101 ttcagaaaca ggttgagcat aaaaattata agaagagctg aaccagatca cgatactaga
     5161 aacaaagata atcgttcctg cttttttaac aaaagacttg ctgcggtcat aggtttgtct
     5221 taggaccgct gaccattgag gaaaatgata tgcgggcaat tccatgataa aaggagctgt
     5281 atctccagca aacaaacgtg tttttttcaa gctgattcca gagaaaataa ttgcagtcat
     5341 accaagaaaa taggcagaag gagctaccca agattgatga ggaaaaaagg cccctgctac
     5401 taaggcaata atcggcaact ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa
     5461 acgacgatct tgctcgtttt caatcgtgcg actggccatg acgcctggca ctccacatcc
     5521 tgtagcaatc agcataggaa tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg
     5581 atccatgaca aaagcgatcc tcgccatata tccgcagtct tccagaaagc tcaagcataa
     5641 aaacaatata atcaattgcg gtagaaatcc taacactgct cccacgcctg cgatgatgcc
     5701 gttcaagatc agctcctgca tccatcccgc tacttgccat tcagctaaca atctgccaac
     5761 atgttctggt aaccatgtac caaacaatgt gtcattgatc caatctgtcc ccattgttcc
     5821 tactgtttga atagccagat aatagatcag ccacatcaca aatgcgaaga tcggcaaggc
     5881 taaccaacga ttcgttgcaa tctgatcgat tttgtcactc atcgataatt ggaaagaatc
     5941 atttgacaca acacagagtg tacatagttg ggtaataaat tcataacgtt cattgaccag
     6001 aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac tggatcagtt gctcgatctc
     6061 tttttgctga gaaactgcta tctcgtaatc ctctcttacc ttctgatctc gttcaaaata
     6121 tttcattgca taccagcgtt tcttgccgct ttgttctcct agtacatctt caatttcatg
     6181 taaagctgct tctaaacgtg gatcatatgt acagggaaca ggaatagaag gccgcttttt
     6241 tgcagactcc tgaatctttt cgattagttt ttcgattcct tgctttttca atacgctgat
     6301 tggtaaaact ggtacaccaa gaccatagga aagcttttct agattgattt tttttccttc
     6361 ttttttgatg acatccatca tattcagacc aatgatcata ggaatgtcta aatccaataa
     6421 ctgcgtagtt aaataaagat ttcgttctaa attggtagca tcaataatgt tgatgatcag
     6481 ttcaggctgt tcctgaacca aataatctct tgcaatcact tcttctggtg tataaggaga
     6541 caaagaatag atgccgggta ggtcctgaat atgaatgttc ggattttttt tcaacgatcc
     6601 ttcttttttt tctactgtta cgcccggcca attccctaca tattgattcg aaccagtcaa
     6661 gagattgaac aagctagttt tcccgctgtt tggatttccg actaacgcga tattcattct
     6721 ttttcctccc tttgtatcaa gattagttcg gcctctgact ttcgtaacgt cagttcatac
     6781 cctcgcactg tcagttcgat tggatctccc agaggggcta gttttcggat aactattttt
     6841 acatttcgcg tcaatcccat atccattaac cgtcgcttca ctgcgccttg tccgtggatt
     6901 gctacgacga tcccagtctc tcctgctttt aattgatcaa gagaagtcca gctttcagag
     6961 gaagcagtct ttttattgac aaagatttga cctagtattt cccgacttac tgccactcga
     7021 tgacttttta ccagtaaaat ggcattgttg cctgataaat tgacgacgac gattttactt
     7081 ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt
     7141 tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg tctgcatatt gattcctctc
     7201 ttgtgaaata aatcatgtat taaaatcatt cattatatgt tctaattttc ttacgtgtta
     7261 cgcaaataaa tgagaatcat tctcacatag aatactctcg gtaattgaga atgtcaatta
     7321 tacttgtgat attatgagcg aatctttagt attgatagat aaaaatgaga aaaaaatatt
     7381 tcttcatagt tttccacaat tttttctcta ttaaatttgc tattttccca cacgatactt
     7441 gttttcagct cttttttcaa aaacacaaaa cttagtattc cacagtttcc acaacacaat
     7501 atgtagtgtt tgttgtttga cttttttact aagagtttta tactatctat tatagataat
     7561 aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa tcttttctaa aaataattgt
     7621 atgcaatgta agatggtcaa acggttttta acagaaaatc aaattgcttt tgaagaagtc
     7681 aatatcgatg aacagcctga agcagtagac tggttgaaag cacaaggatt ccaaagtgtt
     7741 cctgtcatta cttctgatgc agaaacagtt atcggattcc gtcctgatca gttgcgtaaa
     7801 cttgccagct aacatgacta aaagaatcaa aacgtgatac atggatttga agggcttgag
     7861 acagcaccag tttttgtcgc aaacttccaa atcctttttt aaagcttaaa aacaatgcaa
     7921 tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt gggcgtacgt atgagtctta
     7981 aaaatttaca agatgtcagt tatttcaagc tgaataatga aatcaatcgg ccagtcaatg
     8041 gtcaaatccc tttgaataag gataaagaag ctttagttgc cttttttgag gaaaatgtca
     8101 aaccaaatac gatgactttt ctatctatta tggaaaaaat caattacttg atcgatcaag
     8161 aatatataga agcagaattc attcaattgt attcaccatc atttattgaa aagctatatc
     8221 aatttttgga cgagcaacat ttttctttca aatcttttat ggctgcttac aaattttatt
     8281 ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct ggaaacttat gaagatcgag
     8341 tagccttcaa tgcgctttac tttgcgaacg gggatgaaga attagcactt gctcttgcag
     8401 acgaaatgat ccatcaacga tatcagccag ctacaccttc tttcttgaat gctggacgta
     8461 aacgtcgggg cgaattggtg tcttgtttct tgactcaagt cactgacgat atgaactcta
     8521 tcgggcgctc aatcaactct gctttgcaat tgtcccgtat tggtggcggc gtagggatta
     8581 ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg atatgagggt gctgcaagtg
     8641 gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag ctattctaat caattaggtc
     8701 agcgtcaagg agctggcgtg gtttatttga acgttttcca tccagacatt attgctttcc
     8761 tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt aaaaactctt tctcttggcg
     8821 tgatcgtacc tgataaattt tatgaattga cacgtaaaaa cgaagatatg tatttattta
     8881 gtccatatag tgtcgaaaaa gaatatggtg tcccattctc ttatgtaaat attacagctg
     8941 agtatgatca tctagttgca aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc
     9001 ttgaaaatga aatctcaaaa ttacaacaag aatctggcta tccttacgta atcaatattg
     9061 atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat catgagtaat ttatgttcag
     9121 agattttaca agttcaaacg ccttctgtga ttaacggcaa acaggaatat gaagtattag
     9181 gaacggatat ctcatgtaac ttaggctcga caaatatcgt gaatttgatg caaagcccta
     9241 actttggtca atctgtgcgt acgatgactc gtgcattgac atttattaca gatgcatctg
     9301 atatcgatgt cgtaccaccg atccaaaacg gaaataagct gaatcatacg attggattag
     9361 gtgccatggg cttacacact tatcttgcaa aagaacaaat ggaatatggc tcagaggatt
     9421 cattagactt tacaaatatt tacttcatgc tgttgaatta ttggacactt gttgaaagca
     9481 acaatatcgc tcgtgaaaga aaacaagttt tccataattt tgaaaaatca gcttatgctg
     9541 acggcactta tttcgaaaaa tacgtaactg gagaattcca gccaaaatca gaaaaagtca
     9601 aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag
     9661 cagtaatgaa agatggactg tatcatcaaa atcgcttggc agtagcacca aatggctcaa
     9721 tttcatatat caacgatacg agtgcaagta ttcatcctat cactcgcatg atcgaagaac
     9781 gccaagagaa gaaaatcggt aaaatttatt acccagcgcc ttatctatcc aatgagacga
     9841 ttccgtatta cacttctgct tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa
     9901 tggcacaaca acacgtcgat caaggcatga gtttgacttt attcatgcgt tctgaaatcc
     9961 cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac gacacgtgat ttgaatattt
    10021 taagacatta cgctttccac aaaggaatca aatccattta ttacgtacgt acctttactg
    10081 atgatgcaga agaaatcgga agcaatcaat gtgaaagctg cgtgatctaa tatcatagat
    10141 agattcgctt attcatattt taattaaagg agcattctca tgtcagaaac atattatgaa
    10201 gcaataaact ggaatgaaat tgaagatatc attgataaat ccacttggga gaagctgaca
    10261 gagcaatttt ggctagatac acgtatccca ctttcaaatg acctagatga ttggcgtaca
    10321 ttgtctcaat tggaaaaaga tacagtagga catgtatttg gcggtttgac actgctagat
    10381 actgtccaat ctgaaagtgg catggatcag ttgcgtaaag atgtccgcac tccccatgaa
    10441 gaagccgttt tgaacaatat tcaattcatg gaatccgttc atgccaaaag ttattcttct
    10501 atttttagta cactgaatac gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg
    10561 aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa tctacaaaca cggtacaccg
    10621 ctagaaaaga aaattgccag cgtcttttta gaaacattct tgttctattc aggtttttat
    10681 actcctcttt attatttagg aaacaacaaa ttggctaacg tagcagagat catcaaacta
    10741 atcatccgag atgaatctgt acatggaacc tacatcggtt acaaattcca attaggattc
    10801 aacgagttac ctgaagcaga acaagaaacg ttaaaagact ggatgtacaa tcttctttat
    10861 gagctttacg aaaacgaaga acgttataca gaagaattat acgatccaat cggctggaca
    10921 gaagaagtca agacattcct acgctataat gccaacaaag cattgatgaa cctagggatg
    10981 gatccactct ttccagatac tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca
    11041 acaggcacaa gcaaccatga cttcttctca caagtcggaa acggctattt gttaggtcat
    11101 gtagaagcga tgaaagacga cgattatttg attggattgg aataaggatt tattttaatt
    11161 caaaaatttt tcgaaaaata aaaagagttt agcgtacaga tattttaaat gtacactgaa
    11221 ctctttttta tgcttattca cttgtttctt ccagcacttt tggactgtct aacttttccg
    11281 aaacatgttc cattcttctc aataaataga ttttccggat atttaatatt atcgtcttaa
    11341 tcactgcata gacaggtatg caaatcagca tgcctaatat tcctgcgaca cttcctgctc
    11401 caatcaatac caagatgatc gtcaaaggat ggatattcat tgatttccca aataacaacg
    11461 gcttgatcaa atttccatct aattgttgga tcactaaaat cgacaacgcc atatacaaag
    11521 cttgaaatgg agagataaac aaaccgacaa tgattgctgg taccgctcca ataaaagggc
    11581 cgacatacgg gataatattc gtgattccgc aaatgaccgc aagcaacaag ctataaggct
    11641 gatcgaatat tctcattgcc aaaaagctca ttacaccaat aatcaaagca tctaagatcg
    11701 tactgctgat ataggcagat aatgtttcgt ttaattcttt taccgtttgg cgtaactctt
    11761 tacgaatcgc tttcggaaag aagtgagaca acgcatcaat aaatttgtgc ccatccttga
    11821 acataaatag caaaataaat ggaacggtaa ataacaaaat aaaaaattgc atcataaatg
    11881 aaatgatttt tgagacactt gcagataaac tgactaatat gatattgaac aaattactaa
    11941 gagataaatt tgctgatgct aattgttctt ccaaattgaa atttttaaac tcttcccgct
    12001 gcgccaattc atttaaccat ttgcttgttt catctgcata cattggcaag ctctgggtca
    12061 attgaattgt ctgttccatt aactgaggca ctacattcat gacagttaaa acaactaaaa
    12121 tgattacgac caaaaacgtc aataaaaaac cgaacactct cgggatccga tgcttttcga
    12181 gaaaaacaac gattggatcg aacatataat agataaaacc agcaacaagc aatggtaaaa
    12241 aaactgccga aagcattttc aatattggac taaaaatatt cggcatttgt aaaataaagt
    12301 acacgccgat gatcgtgagt actagctcca ctgtccagaa aaataatttt gattgtttaa
    12361 aacgtgattg catacatctc ctccaaggtc ctttgatact tctattctat catttaacaa
    12421 agagttacgc gctgagattt tcatttgctt aaaaaaaaca aaaaaaataa acatatttta
    12481 atgagtagac gagtacttaa acaaaaacac ggtttggagt agtgacaaaa attatactgc
    12541 agtccctttt tccaaataaa cggtgttcaa aagcttgcat cttcgtaata agcccagcca
    12601 aacgaaaaat ataaggaact atttttattt ggctgggctt attacttgat gtaggacatc
    12661 tattttacaa cctcagatca tttattgcgc aatactgact aaatgcaact ttgtgatatt
    12721 tttgtgtacg gacaactcct cgatcacatc ggcataagtg agcccttttg gaagatcaat
    12781 cgtatagata tttttgtaca ctcggtagtc atcaacgaat tttacgtcaa aattcacatc
    12841 ttcgattacg atatttttct cttcaaaata atcggtaaga aaatctttgg tttcttttct
    12901 atggataaat tggatttcta actttttagt cgtaggtaca tggatcacgc gtttcactac
    12961 tgtgagtgca atagcaatcc cgataaaact gatgatcgca atggaatagt aacccatgcc
    13021 gatggcgatc cctaatccag caattgccca taaagaagcg gcagtcgtaa gtcccgtcac
    13081 cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg
    13141 agcaatcagc cttgcttcat ctgaacgaat cgttccagca agctgtggat actctaaagc
    13201 atctcttaat gcaccactag cgatctcaac ttggatcaat gcaatgatac atgctcccat
    13261 acaaactaaa atatgcgtac gcatacctgc tggacgattt ttatattgtc gttcaaaacc
    13321 gattgctccg ccaaatagca tagcgatccc taaacgtaaa ataatctctg gaattgtcaa
    13381 taccatatca ttcatctaaa caccctccag tccaatatat catgtctgaa aggagcagac
    13441 aaaaaaagtg caatttttac ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag
    13501 tcctttttat gaataaacgg tgttcaatca gctgaccctc ggtattactc aacaaacggc
    13561 aaaatatggg gagctatttt tcctttgtct tctctaatac tcaggtcaaa acgctgattt
    13621 cacaacctct ttagcctact attgaaaatt acaacttact tgctgcttct tcatcgatga
    13681 tcacaacaac attgtcgtga ttttgcaata cacttgctgg caaatgattc gttacaggtc
    13741 cttcaatcat ccctttgatt gcatcagctt tgctttcgcc aaatgccatc aagacgattt
    13801 ctttactttt cataattgaa ccgattccca ttgaaacagc ttttgttggt acatcttctg
    13861 ctttatcgaa gaatcgtttg tttgcattga ttgttgattc tgttaagtca actactgaag
    13921 tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat atgtccattt tgcccgattc
    13981 ctaagatttg gatatcgatt ggatgtgcat cgatgatttg atcatacttt ttgcatgctg
    14041 cttccagatc ttctgctttt ccatctggta caaacgtttc tttgaatggt tttttatcaa
    14101 ataattggac atccatgaaa tgacggtagc tttgttcatc tgaaccgcct agtccaacgt
    14161 actcatctaa gtttacagaa gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt
    14221 tatataaagt aactggtgtg cttcctgttg ccaatcctaa tacttttgca cctttgttca
    14281 taccttcttt gatgatttca aatgcttttt ttccgccttc ttcagcgttt tttacacgaa
    14341 tgatttccat agtgattgct cctttatatt ttggtatagc ccaattatag cattcttttt
    14401 ttctttttac aataattagt atagaccaaa ttgcattttt ttcattttct tgacaataaa
    14461 taggaatccg tgttaggttt cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa
    14521 ccaagcaagt gattcactgt ggacgcttta tgtctgcagt ttttttatga aaaaggagtt
    14581 attatgagta tactgacatt aaacaacatt acccagcaat ttggcgacaa aatcctctat
    14641 gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg ggctgattgg acaaaacgga
    14701 gccggtaaaa gtacattgat taaaatcatc actggtgaga tcttaccaga tgacggacag
    14761 atccattggc aaaaaaatat tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa
    14821 acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa
    14881 aaaattgctt ttctttacca aacatatagc gagaccttcg atgattcctt gctggaaaaa
    14941 gctgggaaac tccaaacgga attagaccaa ggagtgtttt atcaaatcga caccttagtt
    15001 gctgagatga gcagcgggtt ggggatcgat gtcttaggtt tagattcact gttgaaaaat
    15061 ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac tacttttaga aaacccagat
    15121 gttctgatct tagatgaacc aaccaatcac ttagatgatc agcatatcca atggctgacg
    15181 cagtttcttc aggattttga cggtgcgtat atcgtgattt cccatgacca agaatttctt
    15241 gatcagatca ccacccatat cgcagacatc gagtttggaa agattactaa gtatactggc
    15301 catctaaaac aagcattgaa acaaaaggaa caaaatcgcg aaagctattt gcgccaatat
    15361 catgctcaac aaaaacaaat cgaaaagacc gaagcttata ttcgaaaata caaagccggt
    15421 tcccgatcaa cgatggcaaa aagccgacaa aaacaattag ataaaatcga acggctgacg
    15481 cctccagctt cagcaagtaa agctgtcttt gattttccat tctcgcctat tgtcacgaca
    15541 ttagcagtgg aaacaactga tttagtcatc ggatatgatc gaccgctact tgcacccatc
    15601 aatttgacca tccgctttgg tgaaaatatt gccattcgag gcttcaacgg gattggaaaa
    15661 tctactttat taaaaacact gatcggtgag attgaaaaaa taagtggcga tttccacttc
    15721 ccagataaca caaaaatcaa ttacttttca caagatctgt actgggaaaa tccattagaa
    15781 acaccactac aatatttaag cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc
    15841 caactagcta aagcagggtt agttaatcaa ttagcaagtg aacctctcac tacattaagt
    15901 ggtggggaac aaactaaggt gaaacttgca cagttgacaa tgaaccaagg taatttactg
    15961 attctcgatg aaccgacgaa ccatatcgat caagaaacga aagaaagctt acaagacggg
    16021 atacagaaat ttccaggaac agtaatcatc gtctctcatg aacaagaatt ttatcaagat
    16081 cttgtgcaac gagtcattga gattgaacca aaataaacgt ttgattgaat agttgaaaaa
    16141 aaggagctgt gacaaaagtt ttgtcacaac tccttcttct gaataaatgg tggccaatta
    16201 gctgaccttc ggtattagct caaaaaacgg caaaatatga ggagctattt ttatttggct
    16261 attcttatta cttgatgcag aacagctttt cacaacctct tttcgtctat tttttatttt
    16321 cgagatttct ctttccttta aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa
    16381 caagcccgct aaccgttaca cctaaccagc catactgctg ccacattgtc gtccctagca
    16441 atgaaccagc cgcacctcca ataaagtaca taaacataaa tacagtatta tttcggttgc
    16501 ttgctgcttc acctagccct tgtactcgtg tttgattggc gacttgacca aattgattcc
    16561 cgacgtctaa taaaataata gctaaaagca aaatggccaa atggcttccg ccaaccaata
    16621 aagcaataaa gcttattgtt tgcataacaa gtcccatata cacgattttt ctttcagaat
    16681 aagaatctgc cagtcgtccc acgataggtg cagcaactgc tccagacaaa ccaaaaatcg
    16741 ctaatatccc tgcttcgaat gttccccaat tgtaggcggg actgctgata taaaaaatca
    16801 acgtagacca aaagatcgaa aatgttccaa acatgaaaaa tccactgact gccgcttctc
    16861 ttaatagacg ttgagaagca actaaatgcg gcaaactttt aagtgaatca aggtatgaaa
    16921 cagctgtact actttgctgg acagaacgcg gcattttcca gtaaagcaga agtgtaagaa
    16981 ttccgactgc acataaagca aataaataga tcatccgcca agaaaaaaga ctggcaacaa
    17041 taccagatac cgtccgagaa agcaaaatcc ccacgagtaa accgctaagc aatgtaccca
    17101 tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg
    17161 gcacgatcga aagtaatcct attaaaaaag aagcgcatgc aaataacgta aacgaaggcg
    17221 caaaaaaagc ggccaacaga gagacggcag ataacgctgc cattcgaatg atcaattttg
    17281 gacgatccac cacgtcccct aaaggaacta aaaacagtaa ccctaacgca tagcctaatt
    17341 gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac agataaacta gctgctactt
    17401 ttgttccaat cggctggatg taatacatat tggcaacaac aacgccacaa gtgattgata
    17461 atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt actcttcatt gatttccccc
    17521 atttcctatc ttagtttaca acttatggaa aagattggaa atagaaaaat acaactattc
    17581 gccacactta ttatttgtgc ttttgttttc cttgtttttt cttgcgatct ttataaatga
    17641 agacaactaa ccctactagc acagtaacac ttgcaaccaa cgtgactgca gttttgttat
    17701 atccgtgaaa cagagcttct ccaccacacg catacaacac agacgaaggg atgactccaa
    17761 tcacgatcga caatatgatt tgctgtagag gcaatttcat cgcatctgca gcaaaattga
    17821 ccatagaaga tggaataacc ggtatcatat agcctaacat tacacctatt tttggatgtt
    17881 tcatttgtcg gattgcttgc acccaatgat tcgtttcggt tttcttatct aaaaatttta
    17941 agcgatgcat aaggaaaatt gcagtgatgt taccaagagc attacccaaa atgttgatga
    18001 tcgcacctgt cagtggtcca aagctcaaac cagcaatgac tccaactact gaagtaggaa
    18061 tacctggaac agcacacatc accgctgtca atacgatcaa gagtatcgat gcaaaagtcc
    18121 catgcgaacg aatatgtgcg agtagctctg ccttactcgc tctcggattt aaaaataatt
    18181 ggatgtctgt ctgatattct aaatacaacc gatagcccac taccagtatc agaatgattc
    18241 ccaacgagat caatatcaat cggattattt tctttttcat atttcctcct gaaaaaaatc
    18301 tatattcatt aaataatagt ttaacataaa aacaaaaaag caccgaaatc caacatagga
    18361 tctcagtgct tttaaatagt aaaaattatt taacagttac agatgcgcca acttcttcaa
    18421 gtttagcttt caattcttca gcttcttctt tagatacgcc ttctttaact ggtgcaggag
    18481 cgccatcaac tacagcttta gcttctttca agcctaaacc agttgcttca cgaactgctt
    18541 tgataacttt aactttttgg tctccagctg cagttaattc tacagtgaat tctgtttgtt
    18601 cttcagcagc agcagcgcca ccagcagcag ctacagctac aggagcagca gcagatacgc
    18661 caaattcttc ttcaatagct ttaactaggt cgttaagttc taggatagtt gcttctttta
    18721 actcagcaac gatgttttca atgttcaatg ccattataat ttcctccgtt taattgttct
    18781 tttttattta atgctttatg cggcaattaa gctgcatctc catcttcttt gtctgccact
    18841 gctttgacag cgtaagccac gttgcggact ggcgcttgta gtacagatag caacatagat
    18901 agaagtcctt cgcgatttgg caattttgcc aatgctgtga tttcttccaa tgaagagact
    18961 ttaccttcga tgataccgcc tttgatttct aatgcttttg cttctttagc aaattcgtcc
    19021 atgattttcg ctggtgcgac tacatcttcg ttactgaatg caacagctgt aggtccagtg
    19081 aatacttcgt ccatgccttc aaggccagct tttttagctg cacgtgaaag aatagaattt
    19141 ttgataactt tcatttctac gttagcatca cgaagttgtt tacgtaaacg tgttacttca
    19201 tcaactgtta atccgcggta gtcaacgact actactgatg cagctgctgt aaatttttcg
    19261 cttacttcat caacgattgc tgcttttttt gcgataattg cttcactcat ttttgagttt
    19321 cacctcctga attttgatgg aagagttttt tgcactgaaa aggcaagtca cttttatagt
    19381 tactttgctc aaccagtatt attcatcacc tattcacttc gttcatagtg attctactcg
    19441 ctttgctcga aaggaaactc ctttcgcttt gctcaaccag tattgttcat catctcttca
    19501 cttcgttcat agtgattcac aataaaaaac tctatgccac cgcagacata gagggactat
    19561 tgaatatatt caataaacgt cctcggtagg aaattaaggc ttgatgccac ctactgtctt
    19621 cggtacaagt taattattaa cctcaactag cttagcatgc taaactagtt gagtcaagaa
    19681 ttatttttca gttggatcaa tcaacattct aatatttaat tagaaagaag cttggtcaac
    19741 atggatacca ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt gacctttagc
    19801 tgctgatggt ttagctttta gcaatacatc atggattgct gcaaagtttt caactaattt
    19861 ttcatcgtca aatgaaactt ttccgattgg tacatggata tttccagctt tgtccacacg
    19921 gtaagttact ttaccagctt tcacttcgtt tactgctttt gttacgtcca ttgtaactgt
    19981 accagtttta gggtttggca ttaagccttt tggtcctaat actcgtccta gacgacctac
    20041 agtagccatc atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc cgttttggat
    20101 tttttgaacc atgtcgtcgt cacctacgaa atctgcacca gctgcttcag cttcttttgc
    20161 tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac
    20221 aacagcacca cggatttgtt ggtctgcttt tttagggtct acgttcaaac ggtatgcaac
    20281 ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc
    20341 aacaggataa actttacttg aatcaacttt ttttaatgca tcttgcattt ttttgctctt
    20401 tttagccatt ttggttcctc cttgattgtg gttataacgg ttgtacctcc cacgtgttct
    20461 atatctttca atatagagcc aactgagaag ctgctgtctt agggtgtata gattattcta
    20521 cagtgatccc catgcttcgt gcagttcctt caaccatgcg cattgctgct tcaacgttag
    20581 ctgcgtttag gtcttccatt tttaattcag cgatttctct tacttgatcg ctagaaactt
    20641 tcgcaacttt atttttgttt ggttcgcctg aacctttttc gattttagct gcttttttca
    20701 atagaacagc agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag
    20761 aaataacaac cggaatgatc aaacctgctt gatcagctgt acgagcgttg aattcttttg
    20821 tgaatcccat gatattgata cccgcttgac ctagtgcagg acctactggt ggagctggag
    20881 ttgctttacc tgcaggaatt tgcaatttaa cgattttttc tactttcttt gccacgagac
    20941 atacctcctt aagtccgtga tgtggttaat tggagatgca tatttctcct cccacgtttt
    21001 atctgatctg aaaaaatcca gacaaataat gtgcaaaaca atgcacactg gtacagtata
    21061 gcaaatttat ttgttttttc aagatttttt tctgctattt ttacattttg cttgaataaa
    21121 cgctaaattt attctaaaaa aacaacaatt tttcgttctc atttcttctt ttatgttaca
    21181 cttgcgctag catggttcaa cagatgaaag caaacaataa cacggttttt tagacccttg
    21241 tacaaagatt cctacataac aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag
    21301 tgtctttgat attattggac ctgtgatgat tggtccaagt agctctcaca ctgccggagc
    21361 tgctagaatc gggaaaatca tacgtagtat atttggcgaa cagcctgatt ctgtagatat
    21421 ttatctatat gaatcatttg caaaaaccta tcgggggcac ggaacagata ttgcattagt
    21481 tggcggcctg ttagggatgg aacccgatga tgaacgttta gctgattctt tgaaattggc
    21541 atatgaagcc ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa
    21601 ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt tcagtcactg gcatttcgat
    21661 cggcggtggc aatatccaga tttctgaatt gaatggattc aaaatttctt taagcatggg
    21721 tactcctacc ttgatcgttg tccaccaaga tgttccaggt atgattgcta aagtgactag
    21781 tatactttct gagaccaata cgaatatcgg cactatgaca gtgacacggg aatcaaaagg
    21841 tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag
    21901 actcaaacag ctgccaaata tcgacagcgt aaattatttc gattaaggag ctaaaaaaat
    21961 gtttttatcg attgaagaat tagtagatca agcaaaagct tatccaagtg tagcagaatt
    22021 gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca tcgagatcat
    22081 ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag gtgttacctc
    22141 cgtcacagga attaccggtg gtgatgcaaa aaaattagat gattatttaa atgaggcaga
    22201 ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt
    22261 caatgcaaaa atggggttga tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc
    22321 tggtgtgctg atggctgtcc gagatcggct tgatttgaca catgaacagc aattggaatt
    22381 cttatttacc gctggcgctt tcgggttagt catcgcaaac aacgcttcta tcagtggtgc
    22441 agaaggggga tgtcaagcag aagttggttc tgcaagtgca atggcagcag cagctttggt
    22501 ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca gtagccatta cattgaaaaa
    22561 tatgatggga ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg
    22621 taatgcttta ggctcttctc aagcttttat ctctgctgat atggcattag cgggtatcga
    22681 aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc
    22741 gtcgattttc aaagaaacag cagaaggtgg cttagctgtt acgccaactg ctaaacgact
    22801 tgcggaagag attttaaata agagggcgtg ataaagctcc ttaatttcga gtattaagag
    22861 attagttatc ttcataacaa aaaacgaaga gaggactaat tgcccttctc ttcgtttttt
    22921 attaatccat caatttcgta ttttttactt catcgatcgt ttttgttcct gcaagttgca
    22981 tcgtgatcga caattctttg ttcaggtgtt caaaaacaga tgttactcct tctgccccac
    23041 ctagattcaa cccataaatc actggtcggc caatcgcaac taagtcagca ccacttgcca
    23101 gagctttgaa gacatgctct cctcgacgga caccagagtc gaaaatcact ggtacacgtt
    23161 tgttgactac ttcggcaatt tttggcaata cttcaaagga cgcaggacct ccatctagtt
    23221 gacgtccacc atgattggat acccagatac catctgcccc tgcagagata gcgattactg
    23281 catcttctgg cgattgtatt ccttttacga taactggaag atgggtgatt tctttgattg
    23341 ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc ataaatttct gcgattcctt
    23401 ttccttcgcc attaccactt gcactttgtt cgctatacgc agctaagtta ggcattggta
    23461 atggaaattg gaattgattg atgacgtctt cttcacggta accaccgagc gttgaatctg
    23521 ccgttaaaat gatagcttta gcccctgctt ctactgcttt gttcaaaatg aactcattga
    23581 atctgtcgtc tttactcata tataattgga aaaactgcgg tgcgcctggc gctgcatcgg
    23641 ctgcgtcttt gattgtcgta tttgcataag tgctgatgga aaagattgat ccagcagcag
    23701 ctactccttt agctgtatca gcttcccctt tttcatgtgc caatccttgg gccgctgatg
    23761 gcgcttggat aatcggtgta tctaattcaa ttccgaaaac agatgtatgc aaatcggctg
    23821 agtcgattcc gcgtaatatc cgtggcatga ttttctttgt attaaaagaa gtcgtatttt
    23881 ctttcatcgt ccactcatct tcagaaccac cgcgaatata gccaaaagct cctgcttcca
    23941 tgcgctcttt tacacgacct tctagttagg caatgtttac aatatcgatt ggatgttctt
    24001 cattacttgt atgataagtc atagtagatc cttctttcat cttcttgatt acatataaat
    24061 catactaaac ttacaaaaag taagcaaaca tttactctca gatactttta tcgctattct
    24121 ttccaaataa atcgacttac aagctgatct acttcttggt tttcatgaag catactatga
    24181 gaggcatttt cacctgtcac tactttttcc tctatttcat ttccgcgttg cttcaacaac
    24241 gcatagaccg ctaatgcact gtttaacggt actgttccat cactcaagtc tgtttcatct
    24301 agttgaccag caatcaagaa aaatcgagtg gttataggaa tattaccgat catttgctgg
    24361 taatcttgat agcggttaga aacgacagca ggcccctttg ctaactcatc tgtcagagac
    24421 tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact
    24481 tgtggcaatg acgtatcctg tccatacgtt cctaaatagc gtagtgcact aactcctccc
    24541 atggaatgcc ccactatgtt gactttatct atgccgtagt tttctttcag atacaaaaga
    24601 caagttttga tccattctgc ttgattccac tcgttatttt tattatcttc aaacaatact
    24661 tggaccatcg gattgtctgc ttttccagac aaatctcctg ttgcttgtat ttgtccatct
    24721 gttcctactg ttaaaaccaa ttcttttact gcttgctgat ttttttccaa acgccgcaac
    24781 atccctttaa aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg
    24841 gttttttcta tcactttact ggattctgcc gttttttctt tatcagaaac ttcagtagta
    24901 tttatcttcg tcgtctggca accacttaac gcaaacaaac tgataaggac taaagctgct
    24961 gcagttgcac gtttgcataa atttttgatt ggttctgtca tacacctctt cctttccata
    25021 tagaaaaaag aaagacttga aaggccaatc atatgatcga catttcaagt ctctttatta
    25081 atcgatttta tctacttgat cgaaatcaag ttctgtactg gtttcacgtc caaacatatc
    25141 gatgttgact ttcaattttt ctttttctcc gtcaacttct gttacttgtc cttctaatcc
    25201 agcaaatgcg ccttcgatga ttttcacgac ttcgccaact tctacttcta gatcattttg
    25261 tcgcaggctc ataccgatag aacgcaagat atgattgact tcttctggaa gtaatggtgc
    25321 tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac
    25381 aacataccat gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt
    25441 atgaacgatt tctttttctt tcccattttt gacttcagtt tctttttctt caggaacaac
    25501 gacacggaaa ataaagtcgg ccattttcat actttgcgca cgagattcga tatttgcttt
    25561 tactttgttt tcatatcctg aatatgtatg caagacatac cagttttttt caaaagattc
    25621 cattgaaggc tcctcatttc attgtaaaat aaaaaaacct cagtacaccc gaagtttttc
    25681 tttcgcttca ttatagcact aaaagtacgt tgtggctagc gagttatttt aaaatccagc
    25741 caaataagct ttggatggct gtatccatta cgtaaaacat caatccgaaa ataatagaag
    25801 tttcgatgac tactaacgta tctttacgga gttgttttct gcttggccaa gttacttgtt
    25861 tgatttcatc ttttacgctt tttagaaatt tcatgagaca acctcccttg ctactgttta
    25921 tttcgtttct ttatgtaatg tatattgatt acaatattta caaaacttat tgatttccaa
    25981 acgttcgccg ctttttcctt cactgactga ttttgtgtag ttgcgagacc cacaaacgga
    26041 acaagcgagt gctgcttttt tctttgccat ttacttctgc ccccttgctg agaatcagac
    26101 ttatacttca taacagtatc acgtaacttt ttac
//
LOCUS       DARDHD010000019        25047 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000019 DARDHD010000000
VERSION     DARDHD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.019"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="SDA06_000660"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="SDA06_000660"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG4227955.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="SDA06_000661"
     CDS             complement(1174..1869)
                     /locus_tag="SDA06_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG4227956.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="SDA06_000662"
     CDS             complement(1882..2598)
                     /locus_tag="SDA06_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG4227957.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="SDA06_000663"
     CDS             2928..3536
                     /locus_tag="SDA06_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG4227958.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="SDA06_000664"
     CDS             3861..4184
                     /locus_tag="SDA06_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG4227959.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="SDA06_000665"
     CDS             4366..5736
                     /locus_tag="SDA06_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG4227960.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="SDA06_000666"
     CDS             complement(5866..7167)
                     /locus_tag="SDA06_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4227961.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9479)
                     /locus_tag="SDA06_000667"
     CDS             complement(7521..9479)
                     /locus_tag="SDA06_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG4227962.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="SDA06_000668"
     CDS             complement(9656..9922)
                     /locus_tag="SDA06_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4227963.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="SDA06_000669"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="SDA06_000669"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG4227964.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="SDA06_000670"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="SDA06_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG4227965.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="SDA06_000671"
     CDS             13266..13829
                     /locus_tag="SDA06_000671"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4227966.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="SDA06_000672"
     CDS             complement(14082..14603)
                     /locus_tag="SDA06_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG4227967.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="SDA06_000673"
     CDS             complement(14606..15448)
                     /locus_tag="SDA06_000673"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG4227968.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="SDA06_000674"
     CDS             complement(15441..16166)
                     /locus_tag="SDA06_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG4227969.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="SDA06_000675"
     CDS             16342..16638
                     /locus_tag="SDA06_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4227970.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="SDA06_000676"
     CDS             17206..18798
                     /locus_tag="SDA06_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4227971.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="SDA06_000677"
     CDS             18811..19551
                     /locus_tag="SDA06_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4227972.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="SDA06_000678"
     CDS             19719..21068
                     /locus_tag="SDA06_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4227973.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="SDA06_000679"
     CDS             complement(21196..21786)
                     /locus_tag="SDA06_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4227974.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22022..23101
                     /locus_tag="SDA06_000680"
     CDS             22022..23101
                     /locus_tag="SDA06_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4227975.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23322..24674
                     /gene="brnQ"
                     /locus_tag="SDA06_000681"
     CDS             23322..24674
                     /gene="brnQ"
                     /locus_tag="SDA06_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4227976.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7156 a         5131 c         4881 g         7879 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact ctcttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagat aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaataact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccatcg ttttatatag ggaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaggcgg attttataat taacttagcc atttggaaat aaaaaaacgc
    21961 cacgtgaatt ccatgttata ttgaagttac cacaactctc aatagacaag gatgaattca
    22021 catgacgcaa aaccatgata acacagtttc acgcaaggga aaacacctaa cctactcaga
    22081 acgttgccaa atcgccatcc tgaaaaagga aaactattcc aaccgtcagg ttgcggatgt
    22141 cctgggctgc gtgccgcaga ccataaacaa cgaaatcaaa cgcgggaccg ttactcagct
    22201 taaccgccag aatcagaacg ggaaggtcta cgactattat catgacgtct acgacgccga
    22261 tgccgggcag gcggcctacg agaagaaccg cttgaactgc ggcagacggc ccttatgggc
    22321 cgacacggac gccttcattg aatgggcgga cgacatgatg ctgttggaaa aatggtcccc
    22381 ggacgtggtg gtgggctttg ccagaaaaca cgaattgttc gacccggcca ttatccctgg
    22441 gaccaccacc ctctatcagt ggatcgaccg tggcatcatg aaaacgaaga atatggacct
    22501 gctagaaaaa ctgtcccgca aaccgaaatc gtcccgtcat ggcaaccgcc cgaataaacg
    22561 ggtgctaggg aaatccattg aagagcgccc ggaggcggtt ggtacccgga aaaccttcgg
    22621 ccattgggaa gtcgacacgg tcgtaggaaa caagaccaag tcggatgccg tgctgctgac
    22681 gttggtggaa cggcagacac gcttcgaggt cattctgaag ctggccggga aggatgccga
    22741 atcggtcaac caagcggtcc gacagctgag agaacgggct ggtgcgtact tcggccagct
    22801 gttcaagacg gttacctcgg acaacggctc ggagttcgcg ggactgcacg atgccttgca
    22861 ggatgtggca aacatctact tcagccaccc ctatgcttcc tgggagcgcg ggacgagcga
    22921 aaaccaacac aagctgattc gccgtttcat ccccaagggc agcccaatcg gcaacgtgag
    22981 cgaaaaccaa atcttgcgga ttcagcgttg gatgaacgat tacccgcgaa agattttggg
    23041 ctatgcaacg ccgcatgaca cgttcgtcca ggcatttagg caggaacggt tgacggcttg
    23101 acacgtgggc ccttgacaac gagcctccct cggcatggct tgtgtctgga aagctggccg
    23161 gggccagcca gtcgggagac taccatgcct cagctcattg tcaagggccc actcgattag
    23221 aaaaattaga cggttggtgg taacgagcaa ccgggtcacc atgggtggct aacttaaact
    23281 tgaaatttac gacttttaaa aaaaccgtaa aggagttatg tatgacaaaa aaattatcat
    23341 ttaaagacta tctttttata ggctccatgc ttttcggtct attttttggt gctggaaatc
    23401 ttatctttcc cgttcatttg ggtcaagaag caggcgctgc taccttttgg gcgaatctag
    23461 gtttcttggt taccggtatc ggtcttccct tcttaggggt gattgccatt ggtgtatcga
    23521 aaagctcagg tgtatatgaa ttagcaaaac ggataaatca aacatatgcg atggttttta
    23581 ccatcttgct ttacttagtt atcggaccgt tttttgcttt acctcgcctt gcaaccacat
    23641 ctttcgagat tggtttagca ccatttatcg atcctagcag acagacgcta tatctagcaa
    23701 ttttcagtat tgtatttttt gtgctggctt ggtggttttc aagaaaaccg acaaagatat
    23761 tagattatgt tggaaagttc ctgaatcctg catttttggt tttattaggt gctttgcttc
    23821 tccttgcttt tttgaatcca ctaggttctg taaatcaagc gcctattcag ccaaattatc
    23881 aagagcatgc ttttttcaca gggtttactc aaggatataa cacattagat gcgcttgctg
    23941 ctttagcatt tgggatcatc attgtcacaa caatccaaaa catgggtgta acaaaacctg
    24001 ctgaaatcgc taaagacacg atcaaatctg gtgcgatcag cattatattg atgggaatca
    24061 tttatacttt gctcgcttat ttaggtgcta tgagcttagg tagctttgct ctaagtgaaa
    24121 atggcgggat cacgctggct cagattgctg atcattattt aggtacttat ggcagtatct
    24181 tattagcatt tatcgttata ttagcttgct tgaagaccgg tattggtttg attacagctt
    24241 ttgcagaaac attcacagat ttgtttccgc aaagaaatta cgcattcttt gttgcacttg
    24301 ctagtatcct tccttgttta gcggcaaacg taggcttgac gaatatcatt caattttcgt
    24361 tgcctgtttt gatgttcatc tatccattag caatgacact gattttacta gtgttagttg
    24421 gtcctttgtt taagcagcgt acggctgttt atcgaatgac tacttatttt acattgattg
    24481 cttcgatttt tgatgggttg aacgcttgcc cagaaactat caaacaaaca cctattgttc
    24541 aaaatctttt atatgttgcg gaaagctact tgccgttttt caaattgggg atggggtgga
    24601 tcgtaccagc agttatcggt tttgttatcg gactgatttg gagtttcgct aagaaagaag
    24661 aagtagcaga ttgataaaag aggttgtgaa agagacgttc agccttgagc aataaaaagt
    24721 aattttgaaa aatggcttct cacatttttg cataaatttg acttaatacc gagaaactga
    24781 cttttgaaca ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa gtattaagaa
    24841 atattttgtg aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact
    24901 ggcttttgaa caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa
    24961 cggcaaagta gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg
    25021 atcaaacaac atttctttta taatata
//
LOCUS       DARDHD010000020        24516 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000020 DARDHD010000000
VERSION     DARDHD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.020"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..651
                     /locus_tag="SDA06_000682"
     CDS             <1..651
                     /locus_tag="SDA06_000682"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG4227977.1"
                     /translation="EYLDQATALNGARKDQISGKGELNNQITSAIFRYLTQRGIKNHF
                     IEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGVPEGKEFIKPIIEFYYKDDQLD
                     DPFINEEHIYFLQLAKPEEISELKAQALAINEILTELFAKVGLVLVDFKLEFGRSNGQ
                     ILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIPVYQEVYNRLSELTEEKERV"
     gene            651..905
                     /gene="purS"
                     /locus_tag="SDA06_000683"
     CDS             651..905
                     /gene="purS"
                     /locus_tag="SDA06_000683"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG4227978.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            907..1578
                     /gene="purQ"
                     /locus_tag="SDA06_000684"
     CDS             907..1578
                     /gene="purQ"
                     /locus_tag="SDA06_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG4227979.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1579..3801
                     /gene="purL"
                     /locus_tag="SDA06_000685"
     CDS             1579..3801
                     /gene="purL"
                     /locus_tag="SDA06_000685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG4227980.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3786..5225
                     /locus_tag="SDA06_000686"
     CDS             3786..5225
                     /locus_tag="SDA06_000686"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG4227981.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5248..6300
                     /locus_tag="SDA06_000687"
     CDS             5248..6300
                     /locus_tag="SDA06_000687"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG4227982.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6297..6875
                     /gene="purN"
                     /locus_tag="SDA06_000688"
     CDS             6297..6875
                     /gene="purN"
                     /locus_tag="SDA06_000688"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG4227983.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6872..8407
                     /gene="purH"
                     /locus_tag="SDA06_000689"
     CDS             6872..8407
                     /gene="purH"
                     /locus_tag="SDA06_000689"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG4227984.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8423..9697
                     /gene="purD"
                     /locus_tag="SDA06_000690"
     CDS             8423..9697
                     /gene="purD"
                     /locus_tag="SDA06_000690"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG4227985.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9819..10733
                     /locus_tag="SDA06_000691"
     CDS             9819..10733
                     /locus_tag="SDA06_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4227986.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10866..12431)
                     /locus_tag="SDA06_000692"
     CDS             complement(10866..12431)
                     /locus_tag="SDA06_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4227987.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12424..13875)
                     /locus_tag="SDA06_000693"
     CDS             complement(12424..13875)
                     /locus_tag="SDA06_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4227988.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13889..14905)
                     /locus_tag="SDA06_000694"
     CDS             complement(13889..14905)
                     /locus_tag="SDA06_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4227989.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15125..16213
                     /locus_tag="SDA06_000695"
     CDS             15125..16213
                     /locus_tag="SDA06_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG4227990.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16267..17793
                     /locus_tag="SDA06_000696"
     CDS             16267..17793
                     /locus_tag="SDA06_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG4227991.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17795..19000
                     /locus_tag="SDA06_000697"
     CDS             17795..19000
                     /locus_tag="SDA06_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4227992.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19257..20234
                     /gene="guaC"
                     /locus_tag="SDA06_000698"
     CDS             19257..20234
                     /gene="guaC"
                     /locus_tag="SDA06_000698"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG4227993.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20498..21922
                     /locus_tag="SDA06_000699"
     CDS             20498..21922
                     /locus_tag="SDA06_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4227994.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22383..23492
                     /gene="pdhA"
                     /locus_tag="SDA06_000700"
     CDS             22383..23492
                     /gene="pdhA"
                     /locus_tag="SDA06_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG4227995.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23495..24472
                     /locus_tag="SDA06_000701"
     CDS             23495..24472
                     /locus_tag="SDA06_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG4227996.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7916 a         4189 c         5549 g         6862 t
ORIGIN      
        1 gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa
       61 ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata
      121 aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt
      181 atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt
      241 gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag
      301 ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc aaaaccggaa
      361 gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt
      421 gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa
      481 attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat
      541 gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa
      601 gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg
      661 taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa
      721 aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg
      781 agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca
      841 agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag
      901 tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta
      961 tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct
     1021 ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga
     1081 tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa
     1141 gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg
     1201 ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa
     1261 gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca
     1321 gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa
     1381 aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca
     1441 gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta
     1501 gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc
     1561 ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa
     1621 aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat
     1681 atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa
     1741 cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat
     1801 gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt
     1861 gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg
     1921 acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg
     1981 ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa
     2041 gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa
     2101 attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg
     2161 attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat
     2221 gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt
     2281 aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag
     2341 ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata
     2401 caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc
     2461 tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat
     2521 gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa
     2581 gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta
     2641 acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc
     2701 gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc
     2761 gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg
     2821 cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat
     2881 tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt
     2941 agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg
     3001 gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc
     3061 ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa
     3121 gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga
     3181 actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat
     3241 ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag
     3301 gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc agattttgat
     3361 ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat
     3421 ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt
     3481 gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc
     3541 aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa
     3601 actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg
     3661 atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca
     3721 gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca
     3781 atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg
     3841 gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac
     3901 aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg
     3961 ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg
     4021 caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt
     4081 ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg
     4141 ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata
     4201 cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa
     4261 aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa
     4321 tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg
     4381 gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa
     4441 gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct
     4501 ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag
     4561 attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag
     4621 cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag
     4681 ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc
     4741 aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa
     4801 tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt
     4861 caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa
     4921 aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg
     4981 atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat
     5041 gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc
     5101 tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga
     5161 cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct
     5221 tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct
     5281 ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa
     5341 acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg
     5401 gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta
     5461 gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt
     5521 gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg
     5581 gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt
     5641 caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt
     5701 aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca
     5761 ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca
     5821 aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca
     5881 aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat
     5941 gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact
     6001 ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc
     6061 aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt
     6121 cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa
     6181 gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa
     6241 atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga
     6301 gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat
     6361 cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg
     6421 ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg
     6481 attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt
     6541 tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata
     6601 aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac
     6661 gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag
     6721 gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt
     6781 tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta
     6841 gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga
     6901 taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc
     6961 aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga
     7021 agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca
     7081 tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa
     7141 tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa
     7201 accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct
     7261 ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga
     7321 agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact
     7381 agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac
     7441 ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac
     7501 gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga
     7561 aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat
     7621 ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc
     7681 cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga
     7741 agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga
     7801 agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc
     7861 ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac
     7921 agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg
     7981 acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac
     8041 aaaacgtaag ccgactccag cagaagcaaa agcattagcg tttgcttgga gagctgtcaa
     8101 acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc
     8161 cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat
     8221 ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc
     8281 agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc
     8341 tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag
     8401 acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca
     8461 tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg
     8521 aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc
     8581 agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt
     8641 tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc
     8701 gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac
     8761 ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta
     8821 tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg
     8881 tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca
     8941 gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga
     9001 attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga
     9061 tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc
     9121 accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc
     9181 agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt
     9241 gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga
     9301 aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt
     9361 agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt
     9421 tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga
     9481 agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc
     9541 aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa
     9601 aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg
     9661 agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa
     9721 aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg
     9781 tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta
     9841 cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag
     9901 atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag
     9961 atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa
    10021 tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg
    10081 atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg
    10141 attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag
    10201 ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc
    10261 aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc
    10321 agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga
    10381 cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata
    10441 tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga
    10501 acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga
    10561 caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg
    10621 ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt
    10681 ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt
    10741 cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca
    10801 gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc
    10861 gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc
    10921 ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa
    10981 gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt
    11041 tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa
    11101 tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta
    11161 aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa
    11221 ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc
    11281 gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc
    11341 gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga
    11401 tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat
    11461 acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca
    11521 actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa
    11581 ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt
    11641 tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg
    11701 accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact
    11761 attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta
    11821 aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg
    11881 tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca
    11941 agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt
    12001 tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa
    12061 gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga
    12121 taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta
    12181 ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg
    12241 tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa
    12301 gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca
    12361 aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa
    12421 actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac
    12481 agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata
    12541 aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat
    12601 ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt
    12661 gtctataact ttgaattgga tcatctcatc aattttgcat gcggtaatac gaataaatcc
    12721 tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat
    12781 catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat
    12841 atggccgtat cttattttta agatgactag ataatcattg atatggctaa tttctttttc
    12901 aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg
    12961 ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa
    13021 actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc
    13081 taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt
    13141 attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc
    13201 aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac
    13261 ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc
    13321 aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc
    13381 taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga
    13441 atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt
    13501 gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt
    13561 actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc
    13621 ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca
    13681 catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt
    13741 tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa
    13801 agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct
    13861 taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat
    13921 tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt
    13981 gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac
    14041 actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat
    14101 caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta
    14161 aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat
    14221 ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa
    14281 ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg
    14341 gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct
    14401 ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc
    14461 tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga
    14521 aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc
    14581 gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca
    14641 tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt
    14701 agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag
    14761 tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt
    14821 caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg
    14881 ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc
    14941 ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac
    15001 ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa
    15061 aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata
    15121 aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat
    15181 tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat
    15241 cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga
    15301 attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca
    15361 agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat
    15421 cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat
    15481 cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa
    15541 ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg
    15601 aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat
    15661 caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt
    15721 gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc
    15781 gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt
    15841 tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta
    15901 tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt
    15961 taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc
    16021 aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga
    16081 tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc
    16141 tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata aagaatccga
    16201 tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata
    16261 agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc
    16321 cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga
    16381 gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc
    16441 tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat
    16501 agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca
    16561 ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa
    16621 ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat
    16681 cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta
    16741 tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa
    16801 agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt
    16861 atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat
    16921 ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcggg
    16981 atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt
    17041 tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac
    17101 gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga
    17161 gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc
    17221 ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca
    17281 aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat
    17341 attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat
    17401 aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac
    17461 tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc
    17521 aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat
    17581 gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct
    17641 gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg
    17701 atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg
    17761 atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga
    17821 taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag
    17881 catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt
    17941 ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc
    18001 tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat
    18061 ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat
    18121 gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt
    18181 agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg
    18241 actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa
    18301 aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt
    18361 gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg
    18421 tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt
    18481 tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat
    18541 tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt
    18601 agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat
    18661 caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc
    18721 tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga
    18781 actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac
    18841 aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat
    18901 tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt
    18961 tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga
    19021 aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt
    19081 gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt
    19141 tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt
    19201 gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga
    19261 aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt
    19321 ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg
    19381 ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt
    19441 tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa
    19501 agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag
    19561 agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac
    19621 atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg
    19681 tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag
    19741 atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct
    19801 ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc
    19861 cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg
    19921 gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa
    19981 caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa
    20041 aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga
    20101 aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa
    20161 gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg
    20221 gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt
    20281 aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat
    20341 tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt
    20401 tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa
    20461 atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa
    20521 acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag
    20581 gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa
    20641 tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa
    20701 acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat
    20761 cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat
    20821 aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc
    20881 agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga
    20941 agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt
    21001 ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca
    21061 gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga
    21121 gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg
    21181 gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa
    21241 taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag
    21301 tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga
    21361 agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac
    21421 gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac
    21481 gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga
    21541 aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga
    21601 agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat
    21661 aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta
    21721 tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga
    21781 tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat
    21841 caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct
    21901 agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg
    21961 gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa
    22021 gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca
    22081 aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa
    22141 tagaaaaaat gcagatgaaa aataaaacgc tttccttatt gtgattttat tcactaatgc
    22201 tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt
    22261 atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta
    22321 ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac
    22381 aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag
    22441 atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac
    22501 ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat
    22561 tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg
    22621 ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc
    22681 ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt
    22741 ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac
    22801 caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga
    22861 agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag
    22921 gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata
    22981 tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac
    23041 ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc
    23101 ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag
    23161 tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa
    23221 cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct
    23281 tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg
    23341 aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa
    23401 aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa
    23461 ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc
    23521 aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga
    23581 agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg
    23641 agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg
    23701 tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga
    23761 agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag
    23821 aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca
    23881 ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc
    23941 caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt
    24001 tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta
    24061 cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac
    24121 atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat
    24181 atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca
    24241 atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt
    24301 tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat
    24361 cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt
    24421 gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa
    24481 gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DARDHD010000021        23884 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000021 DARDHD010000000
VERSION     DARDHD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.021"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(54..788)
                     /locus_tag="SDA06_000702"
     CDS             complement(54..788)
                     /locus_tag="SDA06_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4227997.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="SDA06_000703"
     CDS             1115..1525
                     /locus_tag="SDA06_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG4227998.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="SDA06_000704"
     CDS             1518..2219
                     /locus_tag="SDA06_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG4227999.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="SDA06_000705"
     CDS             2219..3805
                     /locus_tag="SDA06_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228000.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="SDA06_000706"
     CDS             3824..4045
                     /locus_tag="SDA06_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG4228001.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="SDA06_000707"
     CDS             4042..4803
                     /locus_tag="SDA06_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG4228002.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="SDA06_000708"
     CDS             5183..5692
                     /locus_tag="SDA06_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4228003.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="SDA06_000709"
     CDS             complement(5767..6306)
                     /locus_tag="SDA06_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG4228004.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDA06_000710"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDA06_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG4228005.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="SDA06_000711"
     CDS             7319..8170
                     /locus_tag="SDA06_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG4228006.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="SDA06_000712"
     CDS             8198..8833
                     /locus_tag="SDA06_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG4228007.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="SDA06_000713"
     CDS             8853..9626
                     /locus_tag="SDA06_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4228008.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="SDA06_000714"
     CDS             10034..10831
                     /locus_tag="SDA06_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG4228009.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="SDA06_000715"
     CDS             10809..11222
                     /locus_tag="SDA06_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4228010.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="SDA06_000716"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="SDA06_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4228011.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="SDA06_000717"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="SDA06_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG4228012.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="SDA06_000718"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="SDA06_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG4228013.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDA06_000719"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDA06_000719"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG4228014.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="SDA06_000720"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="SDA06_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG4228015.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="SDA06_000721"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="SDA06_000721"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG4228016.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="SDA06_000722"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="SDA06_000722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG4228017.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="SDA06_000723"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="SDA06_000723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG4228018.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="SDA06_000724"
     CDS             21906..22484
                     /locus_tag="SDA06_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4228019.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="SDA06_000725"
     CDS             22536..23018
                     /locus_tag="SDA06_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG4228020.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="SDA06_000726"
     CDS             23135..23509
                     /locus_tag="SDA06_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228021.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4289 c         5123 g         6663 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtc
//
LOCUS       DARDHD010000022        23842 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000022 DARDHD010000000
VERSION     DARDHD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.022"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            224..1168
                     /gene="mvk"
                     /locus_tag="SDA06_000727"
     CDS             224..1168
                     /gene="mvk"
                     /locus_tag="SDA06_000727"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG4228022.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1161..2138
                     /gene="mvaD"
                     /locus_tag="SDA06_000728"
     CDS             1161..2138
                     /gene="mvaD"
                     /locus_tag="SDA06_000728"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG4228023.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2151..3236
                     /locus_tag="SDA06_000729"
     CDS             2151..3236
                     /locus_tag="SDA06_000729"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG4228024.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3233..4297
                     /locus_tag="SDA06_000730"
     CDS             3233..4297
                     /locus_tag="SDA06_000730"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG4228025.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4546..5349)
                     /locus_tag="SDA06_000731"
     CDS             complement(4546..5349)
                     /locus_tag="SDA06_000731"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG4228026.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5369..6211)
                     /gene="kduI"
                     /locus_tag="SDA06_000732"
     CDS             complement(5369..6211)
                     /gene="kduI"
                     /locus_tag="SDA06_000732"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG4228027.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6227..7234)
                     /locus_tag="SDA06_000733"
     CDS             complement(6227..7234)
                     /locus_tag="SDA06_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4228028.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7241..7885)
                     /locus_tag="SDA06_000734"
     CDS             complement(7241..7885)
                     /locus_tag="SDA06_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG4228029.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7985..8743)
                     /locus_tag="SDA06_000735"
     CDS             complement(7985..8743)
                     /locus_tag="SDA06_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG4228030.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8870..10021)
                     /gene="fucO"
                     /locus_tag="SDA06_000736"
     CDS             complement(8870..10021)
                     /gene="fucO"
                     /locus_tag="SDA06_000736"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG4228031.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10103..10414)
                     /gene="rhaM"
                     /locus_tag="SDA06_000737"
     CDS             complement(10103..10414)
                     /gene="rhaM"
                     /locus_tag="SDA06_000737"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG4228032.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10416..11261)
                     /gene="rhaD"
                     /locus_tag="SDA06_000738"
     CDS             complement(10416..11261)
                     /gene="rhaD"
                     /locus_tag="SDA06_000738"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG4228033.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11275..12531)
                     /gene="rhaA"
                     /locus_tag="SDA06_000739"
     CDS             complement(11275..12531)
                     /gene="rhaA"
                     /locus_tag="SDA06_000739"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG4228034.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12528..13988)
                     /gene="rhaB"
                     /locus_tag="SDA06_000740"
     CDS             complement(12528..13988)
                     /gene="rhaB"
                     /locus_tag="SDA06_000740"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG4228035.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14141..15010
                     /locus_tag="SDA06_000741"
     CDS             14141..15010
                     /locus_tag="SDA06_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG4228036.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15136..16035)
                     /locus_tag="SDA06_000742"
     CDS             complement(15136..16035)
                     /locus_tag="SDA06_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228037.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16200..17024)
                     /locus_tag="SDA06_000743"
     CDS             complement(16200..17024)
                     /locus_tag="SDA06_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4228038.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(17035..18330)
                     /locus_tag="SDA06_000744"
     CDS             complement(17035..18330)
                     /locus_tag="SDA06_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG4228039.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18346..18825)
                     /locus_tag="SDA06_000745"
     CDS             complement(18346..18825)
                     /locus_tag="SDA06_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG4228040.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18831..19859)
                     /locus_tag="SDA06_000746"
     CDS             complement(18831..19859)
                     /locus_tag="SDA06_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG4228041.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19876..20982)
                     /locus_tag="SDA06_000747"
     CDS             complement(19876..20982)
                     /locus_tag="SDA06_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4228042.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21211..23808)
                     /gene="adhE"
                     /locus_tag="SDA06_000748"
                     /gene_synonym="adhC"
     CDS             complement(21211..23808)
                     /gene="adhE"
                     /locus_tag="SDA06_000748"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG4228043.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         6727 a         5288 c         4331 g         7496 t
ORIGIN      
        1 ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga aaaaagtgtt ttttctttag
       61 aaaacgtatt cttagtttta cttctgagat ggttttagta aaataaagga ggaatgtttt
      121 ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc aactcgtttt gggcgcttta
      181 ttttttgctt aaaaatcact tttacgtgag aaggagttag attatggcaa actatggcca
      241 aggagaatca agcggaaaga tcatattgat gggcgagcac gcggttgttt atggagaacc
      301 agcgattgcc tttcctttct atgcaacaaa agtcaccgca ttccttgaag agctggatgc
      361 aatggacgat caactggttt cttcctacta ttcaggaaat ttagccgaag ctcctcatgc
      421 attaaaaaat atcaaaaaat tattcattca cttaaaaaaa cagcatgaca tccaaaaaaa
      481 cttgcaactg accattgaaa gcacgattcc tgctgaacgt ggaatgggat caagcgctgc
      541 agtcgccaca gcagtcactc gtgcttttta tgattactta gcatttcctt tgtctcgtga
      601 aatactatta gaaaatgtcc agctttcgga aaaaatcgcc cacggtaatc ctagtggaat
      661 cgatgcagcc gctactagca gcttgcagcc gatttatttt acaaaagggc atcctttcga
      721 ctacttttct ttgaacatcg atgctttttt gattgtcgct gatacaggaa tcaaaggaca
      781 aacaagagaa gccgtcaaag atgtcgctca cctctttgaa acccagcctc atgaaactgg
      841 acaaatgatc caaaaattag gatacttgac gaagcaagca aaacaagcaa tcattgaaaa
      901 ttcaccagaa acgttggcac agacaatgga tgaatcacaa tcacttctgg aaaagctgac
      961 aatcagcaat gattttctta atctattgat ccaaacagca aaagataccg gggcattggg
     1021 tgccaaatta actggcggtg gacgcggtgg ctgtatgatt gcattagcac aaacaaaaac
     1081 aaaagcccaa gaaatcagtc aagcacttga agatgcgggt gctgctgaaa cttggataca
     1141 aggattagga gtacatacct atgtttaaag gcaaagcacg cgcatatacc aacattgctc
     1201 taatcaaata ttggggtaag aaaaatgaag aactcatcct tccaatgaac aacagccttt
     1261 cgttaacatt ggatgcattt tatacagaga cagaagtcat cttttctgat agctatatgg
     1321 tagatgaatt ttatctagat ggcaccttgc aagacgaaaa agcaacaaaa aaagtcagtc
     1381 agtttcttga cctttttcgt aaagaagccg gcctctcact aaaagcttca gtaatcagtc
     1441 agaatttcgt tcctaccgca gctggattag cctcctctgc cagcgggcta gctgctttag
     1501 caggcgcttg caatactgct cttaagcttg gattagatga tctctctctt tcaagatttg
     1561 ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg tggtttcgtc gaatgggaaa
     1621 aaggccatga cgacttaagt tcttacgcta agccagtccc ttccgattct ttcgaagacg
     1681 atttagcaat ggttttcgtt ttgatcaacg accagaaaaa agaagtgtcc agcagaaatg
     1741 ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg ctggttagat tccgttgaag
     1801 gggatctata tcaattgaaa caagcaatca aaacaaaaga tttccaactt ctcggagaaa
     1861 cgatggaaag aaacggacta aaaatgcatg gaacgacttt agctgcccag cctccattca
     1921 cttactggtc tcctgacagc ttaaaagcga tggatgcggt gcgacaattg agaaagcaag
     1981 gcattccatg ctactttacg atggatgctg gccctaatgt caaagtttta gtggaaaaca
     2041 gccatttatc tgaggtacaa gaaacattta ccaaattatt tagcaaagaa caagtaatta
     2101 ctgcacatgc tggtccagga attgcgatta ttgaataaag ggagattttt atgattgaag
     2161 tatctgcacc aggcaaactc tatattgccg gagaatatgc agttgttgaa acaggccatc
     2221 cagcagttat cgctgcagtc gatcaattcg tgacagtcac tgtagaatct gcacgaaaag
     2281 tcggaagtat ccaatctgca caatatagtg ggatgcctgt acgttggaca agacgcaacg
     2341 gagaattagt tttagatatt cgggaaaatc cttttcatta tatccttgct gctattcgct
     2401 tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc cttttatgat ctgaaagtaa
     2461 caagtgagtt agacagttca aatggccgga aatatggatt gggttctagc ggtgccgtga
     2521 ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt gaatttatct cagttggaga
     2581 ttttcaagat tgcagcacta gccaatttag cagttcaaga taatggttcc tgcggcgaca
     2641 tcgctgctag ctgttatggt ggctggatcg ctttctcaac cttcgaccat ccttggctcc
     2701 aagaacaaga aactcagcat tctatcagtg agttacttgc cctggattgg ccaggtctat
     2761 ccattgagcc attgattgct cctgaagatt tacgtttatt gattggttgg acgggtagcc
     2821 ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc gagagaagat aaaatggtgg
     2881 cttatcagct tttcttaaaa aatagtacag aatgtgtcaa cgaaatgatc aaagggttta
     2941 aagaaaataa tgtaacgttg atccaacaga tgattcgaaa aaaccgacaa ttactgcatg
     3001 atttatctgc aatcactggg gtcgtcatcg aaacgcctgc tttgaacaaa ttgtgtaatt
     3061 tagctgaaca gtatgaagga gccgcaaaat cttctggtgc aggtgggggc gattgcggaa
     3121 tcgtaattgt tgaccagaaa tctggcattc ttcctttaat gagtgcatgg gaaaaagcag
     3181 aaatcactcc actgccgtta catgtctata gcgatcaaag aaaggaaaac cgatgaatcg
     3241 aaaagacgaa catgtttcac tagctaaagc ctttcatgat aaacaaaaaa acgaatttga
     3301 cttcgtacgg atcatccata accctttgcc gcaaatagca gtttctgatg tcgatctaag
     3361 tacacaggca gttgggttta cactaagcag cccattttat atcaatgcga tgactggcgg
     3421 aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc gtcgcaagag aagcagattt
     3481 gatgattgcc actggttcag tcagcgctgc cttgaaagtc ccttctttag ccgatactta
     3541 tacgattatg cgacaagaat atcctcacgg gaaaatcatt gccaacatcg gtgcagggac
     3601 ttctgtcgaa agagccaaag aagctatccg attgtttcac gcagatgcct tacagattca
     3661 tttgaatgca cctcaagagt tggttatgcc tgagggagac cgtgatttta ccaattggaa
     3721 agtacttatt caagaaactc aaacagccat tgacgtcccc ctcatcgtaa aagaagtcgg
     3781 ttttggcatg acaagagaaa cgctcaatga tctagctgct ttaggagttc atacagttga
     3841 tatcagcggt cgaagcggaa caagcttcac ccagatcgaa aacgcccgtc gctccaagcg
     3901 ggaactgagt tatttagctg actgggggca atcgacagtc tcttcattgc ttgaagcaaa
     3961 tgaagcagac acttctatgg aaatcctagc ctccggaggt atccgcaatg cctatgacat
     4021 atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact tcaggaaccg tactgacgca
     4081 tctgatgaat cacggcgtag aagaaactat tatactgatg aagcaatggc aagaagagct
     4141 tcgtctgctt tatactatgg tcggcgcaac gaataccgcg gccctacacc aacaatctct
     4201 gatcttttcc ggaccagtga aggattggtg cgaagcaaga gggatcgact tagtgaagta
     4261 tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     4321 aaacacgtac aaacggcgga agtaaaagca actccttcgg aaataagctg aaattcacaa
     4381 aaatttgaaa agcaattttc gtgaattcct tcttatttct tggagttaaa tgcttctgtc
     4441 ccagcctctt ttttcgaata aatgatgttt aatcagctgt ccttcggtat tcgttcaatc
     4501 cgctgatttc acaacctctc cactaaacag tgtactaaag ctcttttaag gttgttttcc
     4561 gatataggca agtatgcctc cgtccacata caagacatgg ccgttaacga aatcagatgc
     4621 gctagaagcc aagaagacag atggtccagc caaatcgatt ggctcgcccc agcgtgctgc
     4681 tggcgtacgt ccaacgataa attgatcgaa tggatgacgt tcaccgtttt cttgtgtttc
     4741 gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt ccaattccat tacattggat
     4801 attgtattga ccgtattcag aagcgatgtt tttcgtcagc atttttaatc cgccttttgc
     4861 cgcagcataa gcactgaccg tttcgcgtcc taattcactc atcatggagc agatattgat
     4921 gattttaccg ccgccttttt cgatcatatc cggaataact gcttttgaaa cgatgaatgg
     4981 ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc gcatccattt cgatcatcgg
     5041 tgtccgtttg atgatccctg cattgttgac tagaatatca actgaaccta catcttcttt
     5101 gatctgttga accatcgctt gaaccgcttc ttcatcggtc acatcgcaga cataaccttt
     5161 tgcatcgatg cctgcttctt tataatgcgc tataccttca tcaactgatt cttgattcaa
     5221 gttattaaat acgatttttg ctcctgctgt agctaaagag cgtgcaatct caaatccaat
     5281 accatagaca gcaccggtga ctaatgctac ttttccatct aaacgaaaca tatccatact
     5341 aaattccatc attttctctc ccttatgctt attttaattg atccatttgc accatatcca
     5401 tgtctgtata ggtgatattt tctccgcaca ttgcccagat gaatgagtaa ttgcttgtac
     5461 ctacacctga atggatcgac cagcttggcg aaataatggc ttgttcattg tctacaacta
     5521 aatgttttgt ttcatctggt ttgcccatca tgtggaacac acgtgtatct tcattggcat
     5581 aatcaaagta gacataagct tccatacgac gttcatgtgt atgacaaggc atcgtattcc
     5641 aagcgcttcc tggttctaaa atcgtataac ccatttgtaa ttgacagctt tcgcatacat
     5701 ttggatggat gtattggtag attttacgtt cattcagtgt caggccttca ccagtctcca
     5761 ttggttttac ttggtcgata ctgattttta catttggata tttatgatga gctggaacag
     5821 aactaatata gaatttagct gggttttcag catcttgaga agaaaataca acatgtcgtg
     5881 tttcttttcc gatatagtat ccgtcttgtt ttttcatttc ttctttttgc ccatcgatct
     5941 cgatgaatcc tggtccaccg atattgatca cacctagttc acgacgctct aagaagtaat
     6001 caacacctag ttctttgtct aatgtgattt ctaattcttt ttcagtaggt gtcactccac
     6061 caaagatcat tcgatcgttg tgcgtataag tcaaactgat tttccctggt tcaaatacct
     6121 tttctactaa aaattctttt ctcaattgtt ccgttgaata atgacggata tcttctggac
     6181 tgtgtgtata acgtgtttcc atttcttgca ttttcttcta ctccttctat cgaatgattt
     6241 ttccagagcc gcatgctaaa aattgtgcga cttcttcttg actaaattgg ttgcagtccc
     6301 catggatcgt atgcttcaag gcagaagctg ccgtagcaaa atcaatgatt tcttgcggag
     6361 acatattgga taaaatccca tggagcacgc cacctgcaaa agcatctccg ccacctactc
     6421 gatccacgat tggggtgatt ctatggactt gggattccac atattcatgg ttcatccaca
     6481 atgtcccctt caattcattt tcacttgctg aatgaacggt acgcttggtc gaataaaaca
     6541 cctgaatatt cgggaacttt tcctgcattt tttgataata ataaattaga ggttgttctt
     6601 ctgcttctgg cgctttctcg atacctaaca aataaagagc atccattttt ccagctgagc
     6661 agatatctac taaaggtaaa agctgatgga ttacttcgct ggcttcttgc tgtgtccata
     6721 attttccacg atagttgata tcgaagctga tcaggcagcc agcttttttt gcagcttcta
     6781 tcacctgttt cgtcattttt tgccattttt tagacaaagc tggggtgatt cctgaaacat
     6841 gaaatatatc tattccttgg aataactcct ctagcgacca ttccaaatga tccatttcag
     6901 caaaacttga tccggcacga tcatagatca ctgaggcggc gcgttcgcca ctaccagttt
     6961 ccatataata agtacctagt cttgatccac cagataatac ctgtttcgta tctactccat
     7021 aacattgcaa gtgctttttg actgcttttc ccaatgcgtt atcaggtact tttgtagcaa
     7081 aaatcgcttc atgtccgtaa ttagctaaag aaattgctac gtttgcttcc cctccgccat
     7141 aatgggcagc gaatcgttca cttgaatcga tacgggttcc ctgctctgtg gacaatcgca
     7201 acatgatttc tccgagtgtc actacttttc ccataagcag ctacctcttg atttctttca
     7261 tttttgctgc atactgttgc gcgacttccg ttactttgtc aaaatctcct gtagctgcgg
     7321 gtgctaaaag gttcccacct actccgactg ttacgacacc agcatcgaac cactctttca
     7381 tattgtccag actcacaccg ccagttggca tgatattgac ttgaggcatc ggcgccttga
     7441 aggcagaaat gatacttggt ccataagcgc ttccaggaaa tagtttgacg atatctaccc
     7501 cgcttttcag cgctgttttg atctcggtaa tagtcataca tcctggtaag taaggaattt
     7561 gatacagatt gcacatctct gccgtttctt gatcaaagga agggctaaca atatactctg
     7621 cgccagctaa gatagccaag cgagctgtga cagcgtctaa tactgtgcct gcgccaatca
     7681 ctatttctgg ttggtctttg tagaatgaca aaagttcttt gattacttga tctgcttgag
     7741 ggactgtaaa agtcaactca ataccagtta gaccaccttt gatgattgcg tggcaagcat
     7801 tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat cactccggcg tttttcaatc
     7861 gagataaaat atcaattttt ttcatcttct ccacctattt cctatttaga aattttattt
     7921 tataacttgg aaatctatac ccatattata cggcagaaaa gaaaaattgc aaacgctttt
     7981 ttcattattt ttgaattctt tgctcgattt cgctttttgt ttgcttgagg gcactgatta
     8041 tttgttcttt tgtctcatct gttaaacgat atttcggcat acttacacta aatgcaccaa
     8101 tgatttgttc gtttttcacg attgaggctc ctatacaaaa aatatcacgt tcgatttctt
     8161 catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat ttcctttttc aaccgtaatg
     8221 cattggtgat cgtatattct gtgtaaggga tcaacggatg cttttcaaga taatcagtgt
     8281 actgctcttc actgaattct gctaatacgg ctttgcccat cgcagaattg tataatggtc
     8341 gggtgattcc gatttttgaa gacatgcgga tcgtttgatg ttttggatct agtttgttca
     8401 cgtacaagat ctcattgttg tccaataccc ctaagtggat agtctcatcg atcttttgct
     8461 gcagctgttc taaaaacggt aaagtcaact ctaccaaatc gatttgttca atgtttttat
     8521 tcgcgtagcg aataagcttt gcacctaaac gatagttttt ctctgaattt ttatttacgt
     8581 agccgatcat caacaaagta tctagtattt tcaagactgt agaagaggtc attcccacac
     8641 cctttgcgat cgtctgcaaa gagacatctt ggttttccgc taaatagtct agtattttag
     8701 aggctttgat caatacagtt ccataaggct ttttattttc catataatat tctcctattt
     8761 ctttataaga aaaattatag cggaaatacc atcaatttaa aagagatatc cagatataaa
     8821 ctaccatttc tctttacagg caatttaatg ttgaagatcc gcttcgtttt tacatcaaac
     8881 tttgatataa agcaatgatt tcttctactg tcgtttctct tggatttcct ggtgtgcata
     8941 catctcgcaa agcatcttct gcaattgctg gaatatctgc ttcttttaca cctaattcag
     9001 aaatggtttc tgggattcca accgctttgc tcaaccgatc gatctcacca caagccgctt
     9061 ctcttacatc ttctaaagac atttcagctg ctcctttaat ccctagaaca agagcgatct
     9121 cacggtactt ttctccagta tattctttgt tatatttcat gacagttggc aaaagtgctg
     9181 cacacgctac gccatgagga atgttgtacc atgcacttaa cgggtgagcc atcccgtgta
     9241 ccagtcctaa accgacattt gagaagccca ttcccgcaat atactgtcct aatgccattt
     9301 tttctcgccc attttgatcg ccatctacag aagcttccaa agaatggccg atgatttcga
     9361 ttgcttttaa atgcagcata tcagtcattt cccaagcgcc ttttgtgata aacccttcaa
     9421 tagcatgcgt catagcatcc atacctgttg atgcagccaa ttttttcggc attcccatca
     9481 tcatatcgct gtcgatgaac gcgacgattg ggatatcatg aggatcaaca caaacaaatt
     9541 ttcggtgatt tacttcatct gtgatgacgt agttgatcgt tacttctgct gctgttccag
     9601 aagtcgttgg tatagctaat ataggtaaac atggattttt cgtatcagca acaccttcta
     9661 aactaatgac gtctgaaaac tctggattcg tcaaaataat cccaatggct tttgccgtat
     9721 cgattggcga acctccaccg attgcgatca aacagtctgc ctctgcctct ttaacaaaag
     9781 ccactccatc ttttacattt tggatcgttg gattaggtac gatcccgtcg tataaagcat
     9841 aagcgattgc tgctttatct agaagttctg ttacttttgt tgctataccg tgctcgatca
     9901 atcctttgtc agtaatgaca gctgctttgc cgaatcctcg ctgctgaaat tcagggacga
     9961 tcgactcaat cgctcctttt ccaaaatacg atgtttcatt taaaatcatt cgattactca
    10021 ttttctattc ctcctaattt gtattatttt tcacaataaa aacgaaagaa ccccaagcaa
    10081 ccggttgccc agttgcttgt tgctacagtt caaagactgg catcaattcc caactgactg
    10141 gagaggcatc tggatttgtc tccatgatat cttccatgta cttccaccat ttttgattga
    10201 tagatgtatt tgccattttt tcccataact tttcatcttc gatcactaag taaccaaata
    10261 acaaacttgt actcggatca agatagatcg aatagtcgat gaccccgtgt tcacgaagtg
    10321 ctgtacgcat ttctggccaa agttcatcat gtcttttttt gtattcttct tgtttttctg
    10381 ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga cacctgcttt cgtatgaact
    10441 tcctccaaat aacctgcttt tggagtgaca ccaaacgcat ctgctaaatg ccacaggttt
    10501 tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga cgtacgtata gacttctgct
    10561 gccttttctg ctgtctcgat caaaccaaag acttcatcca tgtcttttcc tgacccgtag
    10621 attccatgtt gtggccataa aaccaatcga gtttctttca ttttttcagc tgttgcttcc
    10681 ccaatctcat ttgttcccgg aaccatccac ggaatgatgg aaattccttc tggaaaaaca
    10741 actaagcatt ccgtacacat ttgccatagc gtacgactaa aggaacgttc gtctaattca
    10801 tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat gcataacgat ccgattatct
    10861 gggtctactt ctaaacgggc gatatgactc atgaaatgac ttggcaactc gcttgtagga
    10921 aggcattcgt tttccagccc ccacaaaagt tccagtgttt tgccatcttc agcaactcga
    10981 acgattccaa gattctcggc aggttcgctc gctacatttt taaaatatct tcctgaacca
    11041 gttacaataa aatagcatcc tgctaaacgg ctggcatcaa aaatcatcga gatgcggcgg
    11101 atcacttttt caggatccaa gaacggtgtg atctcttctt cttttagtaa ataagaaata
    11161 ttcccgccat ttctttcatc ccatcccaag cgataaagat tcgacgtcgt ttcgatcatt
    11221 tctttcacat aaggcgcttg taaaatatct atttttttca atatagccac actcctattt
    11281 tcttaattcc aacacgtctt tttgatattc ctggacttct tccagccaat ccaaaccaac
    11341 tggaacttga tttttgtaac agaaataatt ccatacatcg gcatacggga aatctttcaa
    11401 ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc agctccattt ctttcaatcg
    11461 ctcaactggt tccagcatag ctttcagcag tgctttttgt gtatttctcg taccgatgac
    11521 ccaagcagcg atacggttga tcgttgcatc aaagaaatcc agaccgatat ttgttttttc
    11581 caacaattcg ttacggacta attctttagc gatttcctgc aattcatcat ccatcaaaac
    11641 cacatggtct gaatcccaac gcactggacg ggaaacatgc aacatgattc ctttgctgaa
    11701 taaagctaat gaagataatt tattggaaat cacttctgtt ggatgaaaat gtccagcatc
    11761 tagacaaaca agtttgtcac gagtcaaccc gtaacccata taaaattcat gggaaccgac
    11821 tgtataagct tcagcgccta aaccaaatag tttgctttct actgcatctt gtacataccg
    11881 ttcatctaac tcttctgtaa aaatttcatc gagtgattcc attaaacgtt cccgtggggt
    11941 aaaacgatca atcggattat ctttcatccc atcaggtacc caaaagttat tgatacttgt
    12001 ttgcccagtt tctttcccaa agtactccgc gattttgcgg cttcttttcc cgtgttcgat
    12061 ccagtattca cgaacttctt tatctggatg agccaatgta aagccgtctt tcatcattgg
    12121 atgcgaaaag aaagtcggat tgaagtcaag accgatttct tctttttttg cccactctac
    12181 ccatttttca aaatgtttcg gctcaatttc atttagatcg acagattctt ctgtatccaa
    12241 atagatagca tgaaggttca atttgtgttt gcccggtatc aacgaataag ccttctccag
    12301 atcttgtcta agctcttcag gtgttcgtgc ggctcctgga aaattccctg tcgccatgat
    12361 tccaccggtc aattcgccat ccggtgtcaa gaaacctttg acatcatctc cttgccagca
    12421 atgcatagaa attttgatgt cctgtatctt ttctagtaca gcatctgtat ctacaccgat
    12481 agttgcatat ttttcttttg cttgttcgta atttctttca atcgtgttca tacgattact
    12541 cctttgattt tattcacgta ccattccatc gtgttttgct tattttcatc tgggtaaaaa
    12601 gtttcacatg gaaacgactg tttgattaca tttcttgctt gttctatatt cgaaaactga
    12661 cctgtactga gcatttgcat ggcaacattc ccgattgctg tggcttcact aggtcctgct
    12721 tctacaatga ttccggcata atctgctgtc aattgattca gtagacgatt attcgatccg
    12781 ccccctacga tgtgcagctt ttccaatttc gttccagtta agtgttctac tttgatcagc
    12841 tcttgtgcgt aacaaagagc aagattatca tacacacaac gagcgatagc tcccgatgag
    12901 acagggactg gctgctctgt ttcaaaacag tattgctgaa tctcttctat catatttggg
    12961 ggattcaaaa atcgagggtc attgatttcc atcacttgct gcagcggttt ttcttttgca
    13021 gcaagctctg ccaattccgc ataactgtac tcgtactctt gcattcgggc aacttcctgg
    13081 atcaaccaca tccccatgat gtttttcaaa aaacgcaccg tgttatgggc accccattca
    13141 ttcgtataat tttcagaaaa agcattgcga gaaacattgg cgatctttgt ttcaatccct
    13201 aaaagcgacc atgtcccact gctgatataa ccccaacgtt ctccttgacc tggcgttcct
    13261 aatacagctg acgccgtatc atgacttgct actgtgataa aggtcacatc tggcaaatca
    13321 tattttgaaa attgttcttt ttgcagatct cctagtatcg ttcccggctc tgtcaatggt
    13381 ggaaataatt ccttcgagac accgattttt tcaagaagtt ctatttccca ttcttttgtc
    13441 cctgcattca gcagctgcat agtagatgca ttagttttct ccgtcaccat gttaccagta
    13501 aagacatacc ctaaataatc aggtatcagt aacaatttat gtgcttttgc caaattttct
    13561 ttttcttcag caaacaattg gaacaaggta ttgaaaggct ggatctgaat ccctgtctgc
    13621 tcatacaact tatctagaga atagatttca gaaaactgtt ctactgctcc attggtacgc
    13681 ttgtcccgat aagcaatcgg atcatttatc cgatcccctt gttcatcaat cagacagtaa
    13741 tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa tccctgtctg ctttaatttt
    13801 tcaaatccaa tcaaaatctc tttgataaga taatcgatct tccagcgatc gtgaccgtct
    13861 ttgttccgaa agccgttttt gaaccggtgt acttcttcca agatcaaaga accttttgca
    13921 tccctcgtac ttttcattag tcgtccgctt gatgcaccga tatcgatggc aacaaatgtt
    13981 ggattcattt ctccacctct tttactttat tgttgattag cactttatta ataaagcgtt
    14041 atcatttatt gacaatgcta tcataataca gaatcttata ttttgcttca cactattttg
    14101 gtatataatt gcattatctt gacaagttgg gagtgtttac atggattact gggaaaaagc
    14161 caaccactca tggtcggaag actcaaaaag agtgatcttg acgcctacac aaagaagcag
    14221 gaatttattt ttttatatcc aagaaatcgg ttacttcaaa gcaccaatcc cttactatac
    14281 tgaacgagca catttacctt cttacttgat aaagtttact ttgtctggca aaggttcgct
    14341 tacatataat gataatcagc aacagctaaa agctggagac ttatttttta ttgactgcca
    14401 agaatatcag cgttatcaaa cgatcagcga agaaccatgg gaaatggatt ggatccattt
    14461 gaatggtcag aacgtccaat cattttatca ggagttcaca aaagacggcc ataacacctt
    14521 ccattcaaca ggagatccat cagaaaacaa gatccaccat ttgatcacag aattgatcca
    14581 catgcaacaa ggcacgaatg cccgtaccga tttccagtcc tctgtagcta tccacgagtt
    14641 gctgaatgaa ctgattttgc aaaagtatca gctagatttc gaagaagagg atattcccgc
    14701 ctacgtttac aaactgaagc aatacttaga cgaacatttc aaagaaacga ttacacttga
    14761 tcaattagaa caacaattct tattgaataa atatcagctg agcaaagatt tttccaaatt
    14821 tatcggttct ccaccaatcg attatttgat cagcaaaaaa ctttcttacg ctaaagacct
    14881 tcttcggtat acagaattta cggttcgaat gatttccgaa gaaataggga ttgagaattt
    14941 tgcttacttt agccgcctgt tcaaagcaaa aacaggacta tcgccgagag actttcgtaa
    15001 attcagctga ttcatataat atgtacgtca ttttttcaaa atattttgaa atatatagat
    15061 aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa cagcagctac tgcggttatt
    15121 ttttagtgat cattctcatt gttcatcttc aaaatgttca taatgaacca actgtttctg
    15181 tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac tctctcttga atgctttcag
    15241 aaaatgcgga taagaagaaa aaccactttt actatataca gcactagagc tgcggtattc
    15301 tcgcaacaag tgcttggcat agatcagctt tttcttcatt acatactgat gaaacgtaaa
    15361 tccagtgtac tttttaaatt ctcgtgtgat atggtatttg ctgacaaaaa actgcttttc
    15421 catttcctct aaagaaagcg gatgagataa attttttgag atatattgta acatttgttc
    15481 cacacgctca ttaggaacta cagcattctg ttcttgcgca ctctcttctt tttgtacaac
    15541 ttgattgaga aaaatcaagt attctaccag agcttgttca tattttatgt cttcaccgta
    15601 aattttttta tcaggaggat ggtcgataaa tgataatttt tgcttcaata cttcctgttc
    15661 tatttgcaat acacgactcc tcttctctcc cattggttga aaacaagccg atagattaga
    15721 ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca aagatataga cacgctcgaa
    15781 tacatctgaa gattggcgga cgatacgatg caaatcattg gaatggatga gcagcacact
    15841 tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa gtcgcttctc cctttaatgt
    15901 agcatggatc tcatagaaat catgatgatg atagatcgtt ttattttggc tgacgacatc
    15961 tttgacatga tagatttcat actgatcaga aaccatcgta ttgacttgtt caccgctgat
    16021 tttcttttgg agcatgtgcc gcacctcctt ttgttcactt atgcacaatt ttattatacc
    16081 acgttttttg taatcaaaaa gagctgcttt tatatactct tataaaaaag aagagacgtg
    16141 gcatcttcta gccccgcccc ctccattcta aatattagtt aaccaacgtc ttctttacat
    16201 tattcaagaa gacttcttcc ataacgaatc ccatcatctt ttgtttcttc aagaaccaca
    16261 tacaggtacg gacgattctt catgatgatt tctttgattt ttgtgtactc gatcacaccg
    16321 tctcccaaat ttaccggcac gattttttcc tgttcgacac gaaaatcttt caaatgaacc
    16381 gcacatatct tttcaccaaa acgttcaaaa gcttcttcta ccaattggac ttgttcttga
    16441 taatcactac ttgtgatcaa attggtcgga tctaaaatga ttcctagata gtccgaatct
    16501 actgcttgga tcagctgctc tacccttgct aaagaataca ccggatgatt caaacctggt
    16561 tctatcccca ccatcatttg gtatttttct ccagctttta ccaagcgacg gataacggac
    16621 acagcttcag caaatgcttc atctgtgaaa ttttcttccg tatattggat ttccggcaaa
    16681 acacatccag tttccgaagc aaccatcgac gcaccaaagg aagaagcata gcgaagatac
    16741 tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat ctggatgaat catattgata
    16801 tagcaactta aaattcctac ggtcacttct tgcttttcta aaactcgttt gacataattt
    16861 cccatcccag aattgatctc atttgcgcca gaaggtattt caggaaacga aagacctaag
    16921 gcgagctgca gtgtatgaat cccttgttct tttgtttttt ctgcaagatc ttctatcgtt
    16981 tggacttcgg aaagatcatg tcctctaatt gtaatgttca acataactat tctcctaact
    17041 cagcatattg ggtagccaca tgacgatttg cgggaagtaa gtaatcaaaa acaagacgac
    17101 taagataacc ccataaaaag gaagaagcgg tttcagcatt gcctctgctt ttacgcctcc
    17161 tacacttgca ccgacatata gtcctgtacc tactggcggc gtgatggagc ctacacacag
    17221 attcatgatt gaaaacaacc cgtaatgtac aggatcaacc cctacacttg tgacgattgg
    17281 taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca tccataaaca tacccaccag
    17341 taataacaca agattgacta ataacagaat aataaattta ttatccgtta cgcccataat
    17401 caacgaacta attgcctgag gaataccggt aaacgccatg gcgaaagaca tcatggaaga
    17461 agtagccaat aagaacatga tcgtccctga cattgctact gattgaaaca acatattagg
    17521 tatatctttt agttttaccg ttttatagta aaacatagca atcaacaggg agtaaatgac
    17581 agctatcgct gaagcttcta ctgctgtaaa tatccccgtc aagataccac cgataatgat
    17641 gacaatcaat agaaggctag gaatcgcctc ttttattatt tttccagcat gagccatctg
    17701 tcctttttcg acaacagggt atttattctt tttagcaacg atgaatgcta ctagcatgat
    17761 tcctagcgcc cacaaagcgc ctacgagata acctcccata aacaaagcag agatcgaagc
    17821 accaccagca acaagtgaat acatgataaa agcagtactt ggaggaatga ccatacctgt
    17881 cggagctgac gcgatattga ccgctgtcgc aaacttccgg tcatatcctt cttccacttg
    17941 ctgagggatc aacactccgc caatcgcggt agaagcggca atggccgagc tagagatgga
    18001 accaaataaa gcattcccta acacattcgt atgagccaaa gcccccggca cgcgcccgac
    18061 gaacaacatc gcaaaatcca ctagtttctt agctatccct ccattattca tgataatgcc
    18121 cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta ttgatacttc ccaccatttt
    18181 ttgagctgaa ctaaaagcag aaacatcaaa cggtataact aacaataaag tcatcatcga
    18241 tgagaatgca atacttatag caatcggcat ccctaataca agcaacacaa caaatacaaa
    18301 aaataacact aatccggctt ctattgccat tttattcctc catttttata ttttttcttg
    18361 aattgctggc ttgttgctta atgtatcgat tagattgatc aggctataga taatgatgag
    18421 tacccccgat acaggtactg acagataaac atatcccata ggaatactta aagaaggcga
    18481 gatctgcccc attgtcaatg tgacagcatt ccatccgcca tacaccatga tcatcgcagc
    18541 aaataccaga aataaacttt gaacaacaac ttctaaaatt ttactttttt tgccagttaa
    18601 tttttcactt aaaaccgtta cggctaaatg tgctttttgt cccacaacat acgcggaaga
    18661 caacatggca aaccagacaa gaccgaatcg aacaagctct tccgtgaccg tatttggatt
    18721 tcctaaaacc gttctggaaa atacttgcca taaaacaaca gcgaccatcc caacaagcaa
    18781 aacagctccc aggatctcta ataccttgtt taaaatcatt ttcatcacag ctacgctcct
    18841 tgctcttgga tcttttgata aaggtctttg taggtaggat cgtttttgaa tatatcatga
    18901 agtggggcaa ctgcctctcg aaacggtgta ttatctacat cattgaattg gactccatgt
    18961 tcttcttgtg ctaaagcttt ttcttcttca attgcttttg caaaagcttc aatttcaaaa
    19021 accgttgatt ctttcgctgc ttcttcaatg gctttttcct gctcatcagt tagctgttct
    19081 ttgatggcat cattcatgat gatgatatct ggcacacgtg tatgctcatc ataggaatag
    19141 tatttagcca caccaccatg tccagctgta tacaagacaa attcattgtt ttctgatccg
    19201 ttgatcaaat tggattgaag agacgtgtac acttcatcac ttcccatagg tacgggcgaa
    19261 ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt gcatcacacg gatcttcttt
    19321 cccttcaaat cttccggcgt atggatcgga ccgtctacca tataaaagct gcgctgacca
    19381 gaatcataat aggtcaatcc ggtaaatcct aaatttttgg tcgattggta gattggctcc
    19441 attatttctt cattatccat caccttaaag aaatgttctt catcattgaa tagataagga
    19501 actgaaaaaa tcgaataatc tttagagaaa ctttccaacg ctgaggcact tacttttgtg
    19561 aaatctactg ctcctgactg tgtcaattcg atcagttcac gttctccacc cagttgtcca
    19621 tctgggaaat actggatctg tacggaacca tctgtttttt cttttactaa acgcccaaat
    19681 tcattcattg catcaattac tggctggctg ttgcttgcat aagcaaagcg caaggtaact
    19741 atctcattta catctgcatc tgcgacagtt gccacactag aattatttcc gcagccgctt
    19801 agacctagca gacctaggct aagcgaactt gctgcaaaca gcagtgatgt ttttttcata
    19861 tgggtttcct ttcttttaac aataagagac gagcaattct gtcaaacaaa gcaccgttaa
    19921 cgactgtcca taaggcattg ctgtgattcc gatattccga taaaaatcta aactgtcccc
    19981 cattcctgtc ccgattgaaa cgttttgaac ttctcctttt tcatcgattt gttcaagcaa
    20041 tccttggata gcacgataag cgacttcttt atattcttgt ggtaaataac gtttatggac
    20101 cgctttcaaa ataccgtaag caaatcctgc agttgcagaa gattctacgt aagaagaagg
    20161 atcatccaac aacgtatgcc ataaaccgct ttcagactga taagttttta atgcacgcac
    20221 ttgtgcttca agtgtttcga tcaataattt gcgcaatgca tcttctttcg ataattctaa
    20281 gatttcaatg atctctggaa tagcaatcgt caaccagcag tttccacgag cccaaaatgc
    20341 ttcggcatag ttatgatttc cttcaaacgt ccagccgtga taccacaatc ctgttcgttt
    20401 atccatcaag tatttgatat ggatcataaa ctggtaacga gcttcttcta aatattctgg
    20461 gcgattcaac agtttcccga tttttgccaa aggaagcact gtcatcatca atgtatcgtc
    20521 ccacaactga ttcttatttt ccggtccata tgtggcatgc tgtaacccgt cttcatttgt
    20581 ccgaggcatg tcattcatga cccattctgc ccattgttct aaataaggca aatagcgtga
    20641 atctttcgtg tcttcataca agtaagccat agtcaatagc ggtgccattg tattgacgtt
    20701 tttgggcgga gcgccttctt ccatacgatc ctcaaaccat tcattgacca cttgataagc
    20761 gcgaggattt tttgtcatct ggtaattttt ataaatacca tatagaccga caccttgtgg
    20821 ccaattccac actgtccagc ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa
    20881 ttcaccatta tcgtctttga tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt
    20941 cactaattct ttaggtacaa aaatctcttc tttaataatc atctttacgc caccttttta
    21001 aacgctttca ttttatatcc ttaatatagc agtaataccg tccactataa atatcgcttt
    21061 ttgaacaaaa actgtctaaa attgacctaa atgttaaata acaaaagtaa ctttcagtta
    21121 cttggaaatt aaaaaaagaa cttggagaaa caacgttttc caagttcttt ttcatctatt
    21181 ttcttatctt ttttctacgc tttcttttgc ttatccgttg taagcatcaa tgatgatctg
    21241 tttcaattca gagatcaatg gttctttcgg attagctgtc gtacattggt cttcgtaagc
    21301 taattcagcc atacggtcta cggttgtatc tagtgtttct tgtgtgactc cttgagcttt
    21361 tagactcatg ttgatgccga cagattttcc taattcataa acagccgttg ctaaagcttc
    21421 gactaattcc tctgttgttt cgcctttcag acctaagaat tttgcgatat ctgcgtaatc
    21481 tgtatctgca cggaaatagt catatttagg gaacatcgca tgtttttgtg gatcttttgc
    21541 attatagcgg ataatatgcg gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta
    21601 ttccccgccg attttatgtg cgactgagtg acaaatacct aggaatgcgt tagcaaacgc
    21661 cataccagcc attgtagaag cgttgtgcat tttttcacgt gattccatat caggtgtttt
    21721 tactgatttt tctaaatagt caaagactaa cttgattgct tgtaggctca atccacgtgt
    21781 ataatctgaa gccatgactg aaacatagga ttcgattgca tgtgttaaca catccatccc
    21841 ggtatctgct gtgacagatg ctggaactga catgacgaat tgaggatcga cgattgcgac
    21901 atctggcgtc aatgcataat ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat
    21961 gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt gtcgggatac atacgaattg
    22021 tgttttttct ggtttgtcga tcttatatgt ccgtttacgg atatccaaga atttttgttt
    22081 tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc cacattccct tagctgcgtc
    22141 cattgcagaa ccgcctccaa gagcgattac tgtatctggc ttgaagtctg ccatcagttt
    22201 cgttcctgcg tatacggtat ttgtcgatgg gttcggttca acatctgaga agacttcgat
    22261 cttcacatca tttttacgtc gtgccaatac ttcacgaact gtatcgcagt aaccgaattg
    22321 gaccatacca gggtcacaaa cgatcatgac acgttcaacg ttttccattt tctctaagta
    22381 caatagagag ttcttttcaa agaagatttt tggcggtaat ttgaaccatt gcatgttatt
    22441 tctccgtttc gctacagttt tgacattgat taagtttaca gcagagacgt tacgagaaac
    22501 agagttctta ccataagaac cgcatcccaa agtcaatgac ggaatcattt cgttatagat
    22561 atcaccgatg ccgccttctg cagatggtgt attcactaaa atacgacaag ctttcattcg
    22621 aagaccgaat ttgacatgca actcttcgtt ttctgaatgg ataactgctg tatgtcctaa
    22681 tcctccaaga ttcaacatac cttcacacaa atcaaacgca tggtccgtgt tgttagcttt
    22741 gatcatggca agaactggag aaagtttttc tcttgatagc ggataatctg ggcctacgcc
    22801 ttccaattca gcaatcagca ttttcgttcc ttttggtaca ctgattcctg ccaattttgc
    22861 gatttccatc gcagaatgtc caacgattga tggattgacc gcatattttc cttcgttcat
    22921 cactgcatct tctaattttt gcaattcatc tggttttacg atataaactt ggtgtgcttg
    22981 gaattctgct tttactgctg catagatttc tttatccacg atcacagctt gttcagaagc
    23041 acaaatcatt ccattatcaa atgttttcga aacgatcaaa tcgttcactg cacgtttaat
    23101 ttttgctgtt ttttcaatat aagctggtac gttacctgga cctacaccta aagctggttt
    23161 tcctgtagag taagcagact tgaccattcc agctccacct gtcgctaaga cgatcgcgat
    23221 acctggatgg ttcattagca tagaagtcgc ttcgatcgaa ggatgttcga tccattggat
    23281 acaattttca ggagctccgg cagcgatagc agcatcacgt accactcgtg ctgcttctgc
    23341 agaagatttt tgggcacttg gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc
    23401 gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt gttggagtta ctccgcaaac
    23461 cacaccgact ggttcagcaa tttcaatcaa ccctttttgg acatcttcat tgatcacacc
    23521 aactgtttta tcatgtttga tattatgcca aatatattcg gacgcataca tatttttgat
    23581 tgctttatct tcgtaaacac cgcggcctgt ttcctctaca gccatcttcg ctaatggcat
    23641 atgttgatcc aaagctgcca ttgccatttc atgcacgata tggtctactt tttcttgatc
    23701 aaagctttcc atttctttca atgctgcatt tgctttttct gccaaatcat caatcattgc
    23761 ttgtacgtca attgtcttcg ttttttcttt ttctattact ttagccatca ttatcctcct
    23821 aatgggtcat ggttatttgt ta
//
LOCUS       DARDHD010000023        23066 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000023 DARDHD010000000
VERSION     DARDHD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.023"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(110..565)
                     /locus_tag="SDA06_000749"
     CDS             complement(110..565)
                     /locus_tag="SDA06_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228044.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="SDA06_000750"
     CDS             complement(719..1927)
                     /locus_tag="SDA06_000750"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG4228045.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="SDA06_000751"
     CDS             complement(2039..2371)
                     /locus_tag="SDA06_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4228046.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="SDA06_000752"
     CDS             2519..3394
                     /locus_tag="SDA06_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4228047.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="SDA06_000753"
     CDS             complement(3476..4057)
                     /locus_tag="SDA06_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG4228048.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="SDA06_000754"
     CDS             complement(4063..5322)
                     /locus_tag="SDA06_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4228049.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="SDA06_000755"
     CDS             complement(5324..6370)
                     /locus_tag="SDA06_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228050.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="SDA06_000756"
     CDS             complement(6605..6880)
                     /locus_tag="SDA06_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG4228051.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="SDA06_000757"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="SDA06_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG4228052.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SDA06_000758"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SDA06_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG4228053.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="SDA06_000759"
     CDS             complement(9979..10659)
                     /locus_tag="SDA06_000759"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG4228054.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="SDA06_000760"
     CDS             complement(10708..11343)
                     /locus_tag="SDA06_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4228055.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="SDA06_000761"
     CDS             complement(11409..12812)
                     /locus_tag="SDA06_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG4228056.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="SDA06_000762"
     CDS             complement(12799..13830)
                     /locus_tag="SDA06_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228057.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="SDA06_000763"
     CDS             13861..14082
                     /locus_tag="SDA06_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG4228058.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="SDA06_000764"
     CDS             complement(14247..14984)
                     /locus_tag="SDA06_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG4228059.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="SDA06_000765"
     CDS             complement(15137..16477)
                     /locus_tag="SDA06_000765"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG4228060.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="SDA06_000766"
     CDS             complement(16714..18237)
                     /locus_tag="SDA06_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG4228061.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="SDA06_000767"
     CDS             complement(18615..19226)
                     /locus_tag="SDA06_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4228062.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="SDA06_000768"
     CDS             complement(19556..20272)
                     /locus_tag="SDA06_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4228063.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SDA06_000769"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SDA06_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG4228064.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="SDA06_000770"
     CDS             complement(20831..21628)
                     /locus_tag="SDA06_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG4228065.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="SDA06_000771"
     CDS             complement(21598..21969)
                     /locus_tag="SDA06_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4228066.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SDA06_000772"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SDA06_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG4228067.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6469 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaaaa
//
LOCUS       DARDHD010000024        23042 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000024 DARDHD010000000
VERSION     DARDHD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.024"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..512)
                     /locus_tag="SDA06_000773"
     CDS             complement(<1..512)
                     /locus_tag="SDA06_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4228068.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQV"
     gene            complement(589..1119)
                     /locus_tag="SDA06_000774"
     CDS             complement(589..1119)
                     /locus_tag="SDA06_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG4228069.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1123..1983)
                     /gene="rlmB"
                     /locus_tag="SDA06_000775"
     CDS             complement(1123..1983)
                     /gene="rlmB"
                     /locus_tag="SDA06_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG4228070.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1976..2371)
                     /locus_tag="SDA06_000776"
     CDS             complement(1976..2371)
                     /locus_tag="SDA06_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG4228071.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2368..3780)
                     /locus_tag="SDA06_000777"
     CDS             complement(2368..3780)
                     /locus_tag="SDA06_000777"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG4228072.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3777..4313)
                     /gene="cysE"
                     /locus_tag="SDA06_000778"
     CDS             complement(3777..4313)
                     /gene="cysE"
                     /locus_tag="SDA06_000778"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG4228073.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4399..4638)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4635..6095)
                     /locus_tag="SDA06_000779"
     CDS             complement(4635..6095)
                     /locus_tag="SDA06_000779"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG4228074.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6265..7422)
                     /locus_tag="SDA06_000780"
     CDS             complement(6265..7422)
                     /locus_tag="SDA06_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG4228075.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7456..8826)
                     /gene="radA"
                     /locus_tag="SDA06_000781"
     CDS             complement(7456..8826)
                     /gene="radA"
                     /locus_tag="SDA06_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG4228076.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8889..9416)
                     /locus_tag="SDA06_000782"
     CDS             complement(8889..9416)
                     /locus_tag="SDA06_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG4228077.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9646..10452
                     /gene="proB"
                     /locus_tag="SDA06_000783"
     CDS             9646..10452
                     /gene="proB"
                     /locus_tag="SDA06_000783"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG4228078.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10449..11696
                     /locus_tag="SDA06_000784"
     CDS             10449..11696
                     /locus_tag="SDA06_000784"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG4228079.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11772..12116
                     /locus_tag="SDA06_000785"
     CDS             11772..12116
                     /locus_tag="SDA06_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228080.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12233..12559)
                     /locus_tag="SDA06_000786"
     CDS             complement(12233..12559)
                     /locus_tag="SDA06_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228081.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13174..14376)
                     /locus_tag="SDA06_000787"
     CDS             complement(13174..14376)
                     /locus_tag="SDA06_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228082.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14493..15008
                     /locus_tag="SDA06_000788"
     CDS             14493..15008
                     /locus_tag="SDA06_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4228083.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15207..15653)
                     /locus_tag="SDA06_000789"
     CDS             complement(15207..15653)
                     /locus_tag="SDA06_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG4228084.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15700..17178)
                     /gene="msr(C)"
                     /locus_tag="SDA06_000790"
     CDS             complement(15700..17178)
                     /gene="msr(C)"
                     /locus_tag="SDA06_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4228085.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17532..18161)
                     /locus_tag="SDA06_000791"
     CDS             complement(17532..18161)
                     /locus_tag="SDA06_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4228086.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18145..18609)
                     /locus_tag="SDA06_000792"
     CDS             complement(18145..18609)
                     /locus_tag="SDA06_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4228087.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19096..20364)
                     /locus_tag="SDA06_000793"
     CDS             complement(19096..20364)
                     /locus_tag="SDA06_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4228088.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20595..21332)
                     /locus_tag="SDA06_000794"
     CDS             complement(20595..21332)
                     /locus_tag="SDA06_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4228089.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21399..21968)
                     /locus_tag="SDA06_000795"
     CDS             complement(21399..21968)
                     /locus_tag="SDA06_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG4228090.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22096..22968
                     /locus_tag="SDA06_000796"
     CDS             22096..22968
                     /locus_tag="SDA06_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4228091.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         6472 a         4927 c         4186 g         7457 t
ORIGIN      
        1 acttgcttgt gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata
       61 taataatgaa aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct
      121 tcttttaata aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag
      181 tctggtcctt ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc
      241 ttcagcgcgt tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa
      301 taacttccaa agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg
      361 atccttgctt cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc
      421 aaacgtagta aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca
      481 ttacgtgcag cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat
      541 ggaggaatct tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt
      601 tctcattaaa tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga
      661 attacgtcgg aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt
      721 tttttttatc gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat
      781 catccactgt tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag
      841 ctgaccagcg attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac
      901 atccaactgg tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac
      961 aacgatgact tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc
     1021 tcttgcatcc gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat
     1081 catgttgtag ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg
     1141 ggaaaaagcc tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg
     1201 cccggtcatc gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc
     1261 tttgccttca ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt
     1321 tgtgccggtc atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc
     1381 ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt
     1441 tgttacgata ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc
     1501 acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa
     1561 aatcaaaaag aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga
     1621 taaatattca taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa
     1681 agtttctaat ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc
     1741 tttcagttct tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg
     1801 cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc
     1861 ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct
     1921 attttgtttt ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc
     1981 catttttttc accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt
     2041 gttttgtcaa atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg
     2101 taacatcagc attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa
     2161 gttcttcttc ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg
     2221 cattcgcaga aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt
     2281 gatcaatcag gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg
     2341 ctaaaccgtt caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg
     2401 tgtatcttct aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac
     2461 aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc
     2521 taataactct tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc
     2581 ttctccttgt gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca
     2641 cttagcaagc tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc
     2701 ttcgatgaat cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa
     2761 attctctgag gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg
     2821 taaattggtg gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg
     2881 tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac
     2941 agtgatgaaa ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata
     3001 gccattgtgc atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat
     3061 ttcattttca tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc
     3121 tcctaaatgt tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc
     3181 ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa
     3241 tgggtcttct ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc
     3301 atcgatggat tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac
     3361 atcacctgaa gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat
     3421 atctggcata tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt
     3481 cgtatcttct tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa
     3541 ttcgtttgct gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac
     3601 tttatagcct cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc
     3661 atttccgata tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc
     3721 cttttgtata gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat
     3781 tctgatctcc tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct
     3841 gtagcgtcgt ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt
     3901 ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca
     3961 gttgggtgtc ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa
     4021 atcacatcgt cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg
     4081 aacacgccag ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag
     4141 aattgagcat tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa
     4201 cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt
     4261 gcagccggat cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc
     4321 cactcctttc gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct
     4381 tttaaataag tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc
     4441 aatgattatc tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa
     4501 aacagttttt cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc
     4561 attcattgat accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt
     4621 tctgttcatc gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca
     4681 agcaattcga tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg
     4741 attggcatga aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca
     4801 gctttgacag ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg
     4861 agaacagtag gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga
     4921 tgttcattga aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt
     4981 tgatacaggc taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct
     5041 acacgtccat cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt
     5101 gctttgatgt aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat
     5161 ttacttaggc gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct
     5221 tcgcccacag gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag
     5281 cctaattcgc gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt
     5341 ttacctgttt cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca
     5401 aatgcttcat agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa
     5461 acgtgagtga ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata
     5521 ccatcgcgtt tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg
     5581 cctttcacca tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca
     5641 gattctagac cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt
     5701 cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct
     5761 tcttctgtgc aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt
     5821 tgatagattt ctttacgttc agattgacgg taagggccgt attctccagg attttcagga
     5881 gattcatccc aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct
     5941 tcgatattac gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg
     6001 cgggcaaaca aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt
     6061 ggacttggtg catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga
     6121 ctttgactaa cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc
     6181 cactcagcac aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc
     6241 ttaccagttg gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt
     6301 tttattgtcg attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc
     6361 tgtttgaagg gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc
     6421 ttctacgaca accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc
     6481 ggcttttact actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt
     6541 tgccaacgca ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt
     6601 atagtcattg gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc
     6661 tacttcactg atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt
     6721 ttgcaaggca tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc
     6781 tgagtccgcg atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc
     6841 taagaatcca gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa
     6901 gattttatat ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg
     6961 actagctgtt tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc
     7021 gattcgtgtc gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg
     7081 caatacacta ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc
     7141 taaagtcaat ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc
     7201 acttagtttt tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga
     7261 taataaaaag aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc
     7321 gtttgactgt tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc
     7381 gccgacaatg atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg
     7441 acaatcattt attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc
     7501 aactatctcg attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac
     7561 ttttgtgaag ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct
     7621 gatttctcca gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc
     7681 tttatagctt gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt
     7741 taccccacct gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt
     7801 ttccagcact gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt
     7861 ggcatttccg aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt
     7921 cccttccatt gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa
     7981 aatctgtgaa ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat
     8041 ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg
     8101 ttcaccttca aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat
     8161 cgagccttct ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt
     8221 tttgagtagt tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat
     8281 gtctggctgc gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc
     8341 gatggcacga ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat
     8401 acttccgagg cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata
     8461 taagactttt ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt
     8521 cccgatacct ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa
     8581 cacacggttc agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc
     8641 tgcgattttt gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt
     8701 tgtatcttgt tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc
     8761 caagaactta ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt
     8821 tgccattgta ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct
     8881 ttaacgtttt atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct
     8941 tgatctgctt ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc
     9001 actacatcct tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca
     9061 ttattgtaat aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc
     9121 aacggattgc ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg
     9181 attccagtag gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc
     9241 aatccatctg cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc
     9301 acgacctctt ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt
     9361 agattgatat tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt
     9421 tttcctccct aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt
     9481 tacaaattct tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca
     9541 aacgactgca agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt
     9601 agaatagaac gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt
     9661 aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga
     9721 aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg
     9781 ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat
     9841 ttgtcagaac gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc
     9901 gaactgatga atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc
     9961 ctcgtgacaa gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg
    10021 gaacagcttt tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg
    10081 gatgaattag atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca
    10141 aaacttgtcg cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat
    10201 aatccgacta caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag
    10261 attctggcac aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa
    10321 ttaaaagccg cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa
    10381 aatccggcaa ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag
    10441 gacttgaaat gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac
    10501 tagcacttat gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca
    10561 aaaagaatca acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa
    10621 acaatatcag tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca
    10681 tggctacaga gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga
    10741 tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg
    10801 gcatcattta cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat
    10861 cagggaatgc cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag
    10921 tccaattatt gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg
    10981 tcgatgatac ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg
    11041 tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac
    11101 ctatcattga aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat
    11161 ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg
    11221 cagaaacatt gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag
    11281 aactggtaga acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga
    11341 cggctgcttt agcagaagac acagattggg atactgaata tctagattat atccttgctg
    11401 tcaaagtggt cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac
    11461 attcagaaac gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg
    11521 acgcggcggc tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt
    11581 ttggtgcaga gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa
    11641 atgaattaac ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt
    11701 tcgtgtgaaa cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga
    11761 aaggtgtcaa tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata
    11821 tcgctgcttt tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca
    11881 tagggagtca acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg
    11941 ttacacgctt gcacattagt tacatggttg cattatgctt attagcgaca atcaattact
    12001 attctttttt cgatcattca atcgcttata cgattacgct actgattgct gggatttttc
    12061 acttatctta taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa
    12121 tatccgtacg ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag
    12181 gctgtgacaa agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc
    12241 tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat
    12301 cataataacg aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg
    12361 ctgattcagc cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca
    12421 tcgcttttct agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat
    12481 aaatcaaccg atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct
    12541 gaaaatattc ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt
    12601 acctactgaa aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc
    12661 gtgtcacatc ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt
    12721 cacaatattc aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt
    12781 aaacgacagt ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag
    12841 ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga
    12901 taaaacgctg attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta
    12961 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga
    13021 caaaacgctg attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa
    13081 taagcccaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttatgacttg
    13141 atgcaggaca gctttttcac aacctctgat ttcttattct tcttcatttg tagcaaaata
    13201 attacggtga taaatgatac aacatacact gataagaaca atggcacctg caaaataaaa
    13261 cgcatagctt tcaccaatat gttcaccaat cacacctgca ataaatggtg ctaaagctcc
    13321 accgaaaaag cggacaaaac tatagctgga cgaagcaacg tttcgttgca gacctggtat
    13381 ttccatagca atcgtcgtca acagtgtatt gataattcct tggaaaaatc ctgctaacac
    13441 aatgctgatc ggtacaagga ctggcataga agttccagct cctaataaca gcaaacaaat
    13501 aatgaataag acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc
    13561 cggtgcaacg aaaatcgaag caatagccag aagcacaccc cagccaaaaa atacaaaacc
    13621 gacttctaat tcagaatagc caatcaataa aaatggcgca tacgcaagta aagtaaagaa
    13681 tccgaaatta tataacaatg cgattagtcc aactgaacga agtttatgat tgcttaacgc
    13741 tttgacacct tcccaaaggc ctacttttct ctttggtttc tcagtttcag gtaaaagtac
    13801 cgctagggct ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca
    13861 tgaaccactg cccaagaccc caccgactag agggccaaca gccattccgc agccaagtgc
    13921 tgcttcatac atcatgattg ctttttctgt gttaccaact aaaatactga cgatcgttgc
    13981 taaagcagta gagacaaata aagcattccc gacaccccag ccggcacgta acccaatcag
    14041 cattccgata ttcccagata atccgccaag accagcgaat acaacgatga taaacaatcc
    14101 tgtcatcaat gtttttttca aaccgatccg actagataag aaacctgtaa atagcatcac
    14161 cacaccagtg actaacatgt aactagtaaa taacagggtc gtttcagctg gcgtagcgtt
    14221 taactgtgca gcaatcgttt ttaagattgg gtctactaga cccactccca taaaagcgat
    14281 catacaggta gctgctacaa ctagcgctga acttttcccc tttgtttctt catttgtttg
    14341 ttcttgtata tctatttgat tctccaatat ttccatccct ttctctctat tagcatcata
    14401 catgatttat tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata
    14461 ataaagaaaa taagacgaga ggtaggattt ttatgaatac tttagcctat atattattat
    14521 gcgtgctttt atccaaacca cgttctgggt atgaattgaa gcagctgatc acaatttttt
    14581 gggaagccca tcacagtcag atctatacaa cgcttgccaa gttagaccag cagggttatg
    14641 tgaccgtttt ggacaaagac gagcactctc aaaaaaagat ctaccacttg actgatgaag
    14701 gacgtgctct tgtagaggaa tggatcaaag aggaaactcc cgctcctacg caaaaagatg
    14761 aatttctagc aaaaatctat gctttttcga cgctcgataa aacaacagct actggactgc
    14821 tattcactag agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg
    14881 acctctcgct agacaagaaa caagaatttg gacgttatgt tgtgatccag cgcagaatta
    14941 tgctttgtca gcaggaactc caatggtgtc agcaagtaag agaacaaatg cagcagtttt
    15001 ttgagtaagg gataaacaga acctttctag aaatatctat tgaagagcaa attatcttca
    15061 ataaaagagc aatacagttg gaaaagaaaa agcgatccga acgacaggcc aaaacctatc
    15121 gttcggatca ctatttttct agaactgcat tcaagagact atgacaaaaa gcatgccaag
    15181 gccttttttc aaataacagt gttccatcat tcattacttt gaattgcttt tagcacactt
    15241 cttgaaagcg ggccaacgat gatcaactgc aacggtaaag cggcaatcac attcattttc
    15301 caagcattca tataagcaga gaataattga tcaggtattc cttgttccat gatcacaccg
    15361 aataaagaca tacatgtcac cattcctaaa accatcaagc atgaaatagt taagatcatt
    15421 tggaccttgc tttctggatt tactggtagt ttaaaggcaa tttttttcgc cagcacaccg
    15481 atgatgaaga catccaaaat gaaagccacc acgaatccag gcactagacc aatcaacaaa
    15541 cttgataaat gaagatcttg atgcagccat aaattatacg cgctcatccc caagaccatc
    15601 aaaaaacaca tcattgttgt aaaaattatt ccttcttttt tatttgtagg caatctgtac
    15661 cactccttta aatttgtcac gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac
    15721 tttttttatt ttctgccctt ttatttcata aacttcatca gccgtacgct cgacaaagta
    15781 agaatcatga gaagtaaaca aaatcgttcc cggatatatt tgcattagct tctctaaagc
    15841 ttcgatcgtt gccatatcaa taaaattagt cggttcatct aacagcaaca cattacttgg
    15901 cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct cccccactta atgtacataa
    15961 cggtttcgtc aaagtttcct gagcaaaccc taagtgattc agcaaactac ggacaagcga
    16021 ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg gattcttcag atgtcatttg
    16081 ataacccttt tgctgatata cttgaaagct tactttcgga gagagaagga ttccttctcc
    16141 ttgtttgcgg atatgttcta ataaggttgt cttacctgaa ccattttcac cgacaatcgc
    16201 gattttttta cctaaagaaa actgaaaatc accatttacc agtaatgttc tcccacttct
    16261 ttccaattgg acattttgtc ccatgatggg atagcggctg tggagttcaa gagattttgg
    16321 tactggaaaa cggatttgtt tcatctgctg cggcttggtt gtttctccta ttttttggag
    16381 tcttctctct aatgttttcg cattcttttg gatggctttt tgtacactgt ctttttgttt
    16441 agaggaagac aaccgatcca gtctgatttg ttgctttttc tttttactgg acactttttc
    16501 caacttttcc gcttgttttt tcttttcttg gatcgatttt tccaatcgtt ttttctcttt
    16561 catgatcgac tctgcttctc gtagctgagt ctttttctcc aattcctttt gagtgagata
    16621 ggcactataa ttccccgaaa actctcggat ttctccgtct tttacttccc agattttcga
    16681 tgctagctca tctagaaaat agcgatcatg actaacaaaa accagcgtac cataataata
    16741 acgaagctct tcaatcagtc gatcaatact ttttctatct agatgggttg ttggctcgtc
    16801 tagaagcatc ccttgttcat aagaagaaag aagacgtatc aactcgatct ttcgttcttc
    16861 ccctccgctc catttccgct ctgaagtttc ttccagtctt aactggaaat gactggctaa
    16921 ttccaaattt aggtcctttg cttcagcgag ataggttaat tgaggcaggt agttgaaggt
    16981 gatctctgtt tggattttcc catgatcggg aaaaagttca ccggctatta gtttgagcaa
    17041 agtcgattta ccgacaccat tttctcctat gatccctata cgatcttgct gatagacacg
    17101 aagagaatca atagatagtt ctagctggtt gccaaagctg acttgtagat ttgttatatt
    17161 tactgctaaa ttttccatat gttttacact cctatttcat gtaaaacaac aattatcgat
    17221 actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc atcacatctt ttcctactct
    17281 gaacgaaata actatgacta aatcaccaaa tctgtcatag ctttcaccta ttctactgtc
    17341 gttcgaaaaa atggacatac tccatccatg aaattgttgt ttatacatgt cttttgaatt
    17401 tatttagttg ttattcaaaa gttcgaaacg taatttcatc gatgcagtca tttttccgac
    17461 ctcctttttt attaggttac cacacacgat tcaaaaaaag caatagctac tgaatagtaa
    17521 ctgaaaacct tttattcgaa catagtgatc ttttctggtg ctaaacgaac agactcgtaa
    17581 ccttcttcta ccgcttttcc tattgataaa ataataactg gaacataacg gtctttatcc
    17641 aaaccaaatg cctctgccaa ctgatctgct tcgaatccac caattggatt tgtttcatag
    17701 ccatgatcac gcgcaactaa catgaattgc atagccgcta agctagaatc gactttgact
    17761 acatcgttca tttcttctct tgaaaaactc ttgtaataag gaatgatcgc agctaattgt
    17821 tgatctctga cttcttttgg catcttccct gattcgtaag cttggttgta gatttcttcg
    17881 ccatattcgt aacattgcat atcgccaaaa atcatgacca ttgcagaaga agtatcattt
    17941 tgtctcgtat tgaaacggat caaaggacgc aagatttctt ttgcttcctc gctttcagcg
    18001 accacaaagc gccatggttt catattgaca gaagaaggtg cagtcgtcgc ttctttgatc
    18061 atttccagca tttcttcgtg tgggattttg tatgtttcat catatacgcg aacggatctt
    18121 ctaccagtca taattgttga aaaatcattt ttttgttcca ttattattaa cctctttcgt
    18181 ttctgttgtt atattgcaaa gttgggtatt caatttatct aaaagtgtta gaaattgttt
    18241 catttctaca tcgctgaaag ccggggatag cacggaacct gtatctttat ggtccatttc
    18301 acaatgcttg attttcttct gggctttttc tgttagcgct acatacactt cccggttgtt
    18361 ctgaggattt ctctgacgcg aaacatagcc atttgcttcc aataccttca gatgacgagt
    18421 aactgctcct tgatcaatct gcaacttttc ttgcaaactg ctttgtagca atggattttc
    18481 ttctttcagt ctcatcatga tctcgtatct agtaagactt ataccgatat tttcttcaaa
    18541 gatctggacg attttttgat cggctaattt gatttgatat aaaaggtgac taatctcttc
    18601 taaagacata atttcctcct ttctaataat tgactcgtca acgattgact tgtcaataat
    18661 agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata
    18721 gaaaagtata gagaaaagaa attttttgta gaatgaacgg aaaaaatgat aaagaaagca
    18781 caacgctaaa accaagatta ttgattctta tcaaactata gaagatagct cttctgcagt
    18841 agataatttt acaccgccaa aaatttccga aatcgccttt tgcaagaatc ccgtcgaata
    18901 ttagaaattg gatcagtatg cttaaacagt aataaaaaag ttatgacaag atgttgtcac
    18961 aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa
    19021 caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc agggcagctt
    19081 tttcactacc tctttttaat aaccataaaa tcctattcct tcatatctcc agcccatcgg
    19141 tgttagccaa tctctttcct tagcactcaa ggtatagtga tgggtaccag ttttttcatt
    19201 tggattaaat agtcgataaa ttggcttgga accaccagaa taaaaactta gaccttcata
    19261 acgccaaccg atttttgtta aagagttgat ctcagattgt gccattgtgt aatgatggtc
    19321 tccactgtaa ggattgtata aacggtagac aggttgtccg ctgtttggtg cttgccaagc
    19381 aatcccttca tattcccagc ctacagttgg cagataattt ttttctccgg cattcaatgt
    19441 atataaatgt tctcctgtat gacggttata cagacgatac atcggagaag ttgaagttgg
    19501 tgctgtatca tactgtgtca gattataggt ttcgattaca ttgttcaatg tagaaccata
    19561 attaggcgca gtggcataac gacctgttag ccaagccgta gcatctttgt aagagttcgt
    19621 attgcttttc catgcaccgg aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa
    19681 taccgttacg ttatcttgca atgattcttt gtacgaagga tattttctaa aagcggcatt
    19741 caccgtaatc cattgcccat tcacatattc cgaagtaggc atcgtaacag attggccatt
    19801 atagcttcct ttaatcccaa atagattgta attaggggca gaagccaaag tactgttccc
    19861 ccagccactt tcaacgatgg cttgagcaat catgactgat gcatataagt cattggcact
    19921 tgccaaactt tgtgcagttg aggcaatact atcaatgaac gcttgttgag aattagcaga
    19981 tgagcgagca taaaaagcat agccagtata gttttcgaat tcagacaaat ctgcttgatg
    20041 ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg atttcttctg cattctctgg
    20101 attgttctga gcttctgctg atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt
    20161 tgttggttct tcggttgaaa ttgtattttc tgagacagta gctgactgca aagtactttc
    20221 tgtatcgttt tcaattcctg tttctgaaga actcgatgtc tgagttgcga cagtttctgt
    20281 cgattcttca gctagcactg ttgtgttggc aactgctaga tttcctaaaa gaataacgct
    20341 aaataataaa ttctttgctt tcatcttttt tctcctttga attatgaaac ttttcctaaa
    20401 taacagtgta actaaaaaag taaaagcaag tcaatgtcca tatttaaata acatttgttt
    20461 ctttttaata aatttgcttt ttctgcataa aaagacgtaa atgaagaata ttcttctcca
    20521 ttcacgcctt aaattgatgg tcgtttgaat aactatttaa caagtaattc tctctggata
    20581 ttatccgtta tggattatac ttcctgtgtc gtcggacgga taatcatgtt gctgacagac
    20641 atccggtctg gcgtttctat cgcaaatgct acagcagaag ctatatcttc agatgttaat
    20701 ccccattctt tttgcgcttc atgaagttca agagcagcct ttttgtcgct gatggtttga
    20761 tataattcag tttgaacagc tcctggagag atgatcgtag atttgatgtt attttgccgt
    20821 tgctcttgac gtaatccttc catgattgca cggacagcaa attttgttcc gcaataaacg
    20881 gcggaatcag gataaacaac atgtcccgcc acagaatcag ttgaaatgat atgcccagat
    20941 ttttgttttt ccatgattgg aagtacggcg gaaattccat ttaacacacc catgatgttg
    21001 atatcaagca tattttgcca ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg
    21061 attcctgcat tattgtacaa tacatcgatt cgaccgaact tttccatcgc taaatcgata
    21121 actgcttgga cttgtgcaaa atctcttaca tctgctgttt gaatatatag ctcagcttct
    21181 ggtaaagatt cttttattgc ttttaggcga tcttctcggc gtgcagctat aactagttta
    21241 gccccttttt ctgctaatag cttcgttgtt gcttcaccga ttccgcttga agcgcccatg
    21301 atgacaatca ctttttctga taaatcattc attctattct cctcctgttt tgaaaaatct
    21361 cttgcttata ctcactatct cttatttcat cttactgttc aaataatccg ttcgacaggt
    21421 ttgacgattt tggcagggat tcctgcaacg atggtattgt ctgggacatc tttcgtaaca
    21481 gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag aatagttgct
    21541 cctgctccga tccaagcatt tttcttgatg tggattggct ttactgacaa gcctcttcgt
    21601 tcttttggat cggttagatg gttcaccgtg atgaggttga cacgaggacc aatcaatact
    21661 gaatcttcta aacagatgcc acctaagtca acgaacatca cttgacgatt gataaagaca
    21721 tttttcccta tactaatatg tttgccgaaa tctgtataaa aaggcagcag aacagtcacc
    21781 gtatcatcta ctttgtcact aatgatattt tctagccgtt tgcggatatc tgctggttca
    21841 tggtacccag tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc
    21901 acttcaaaga gcggatccgt tactcgaatc tctttattga cgatttgttt ttcgatgagg
    21961 cttgtcattt tgctccctcc ttttatcctt actttataac ggacggctta tgatgtctaa
    22021 tacttataaa acatacttaa ctattccttt taactatact tagtggtatg cttttttaaa
    22081 aacaggaggg aaaaaatgga tattcgtata ctgacgtatt ttttagcagt agctagagaa
    22141 aaaaatatca gccgagcagc aaaatctctg catatcacgc aacctactct ctcaaaacaa
    22201 ctgaaagatt tagaagaaga acttggtgtg acacttttca ctcgaggctc ccgagagata
    22261 cagttgacgg attctggtcg atatctttat tcaaaaggaa aagaaatctt gtcgttggta
    22321 gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct ctggagaaat ctacattggc
    22381 ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg
    22441 catcctgata tccagtttca tttatatagc gggaatgcag atgatatcat agacaaactg
    22501 gatagcggat tacttgattt tggactcgta atcgaaccga caaacaaaca aaaataccag
    22561 tcatttcgac tgccagccca agatcgttgg ggacttttga tacatcggaa tcatccttta
    22621 gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat catttcccgg
    22681 caaacgtcag tggacagtca actagctgaa tggctaggtt tcagcataga tcagttatcg
    22741 atcgtcggaa catataactt attgtacaat gcttctttga tggtcgataa cgggactttt
    22801 ggtgcattag ctctagatgg gatcatcaat acttatggga cggaaaaagt attcatccca
    22861 ctcgatccgc cattacaagc ttcactgaat ctcatttgga aaaaagacca gcctctttct
    22921 cctgctgctc gtaaattttt agatgtccta acggagaaat acagttaagt agagtttttt
    22981 tccttcgctt tcagcttttt aacaacgaat aagttaagca aaagatccaa tgattaaata
    23041 ac
//
LOCUS       DARDHD010000025        22738 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000025 DARDHD010000000
VERSION     DARDHD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.025"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..3658
                     /locus_tag="SDA06_000797"
     CDS             131..3658
                     /locus_tag="SDA06_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG4228092.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="SDA06_000798"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="SDA06_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG4228093.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="SDA06_000799"
     CDS             7446..7787
                     /locus_tag="SDA06_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4228094.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="SDA06_000800"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="SDA06_000800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG4228095.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="SDA06_000801"
     CDS             9144..11564
                     /locus_tag="SDA06_000801"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG4228096.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="SDA06_000802"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="SDA06_000802"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG4228097.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="SDA06_000803"
     CDS             12998..13735
                     /locus_tag="SDA06_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4228098.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="SDA06_000804"
     CDS             13749..14564
                     /locus_tag="SDA06_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4228099.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="SDA06_000805"
     CDS             14577..15224
                     /locus_tag="SDA06_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4228100.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="SDA06_000806"
     CDS             15240..15896
                     /locus_tag="SDA06_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4228101.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="SDA06_000807"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="SDA06_000807"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG4228102.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="SDA06_000808"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="SDA06_000808"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG4228103.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="SDA06_000809"
     CDS             18238..18756
                     /locus_tag="SDA06_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4228104.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="SDA06_000810"
     CDS             18863..19363
                     /locus_tag="SDA06_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4228105.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="SDA06_000811"
     CDS             complement(19643..20563)
                     /locus_tag="SDA06_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG4228106.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20728..21741
                     /locus_tag="SDA06_000812"
     CDS             20728..21741
                     /locus_tag="SDA06_000812"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG4228107.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="SDA06_000813"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="SDA06_000813"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG4228108.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7577 a         3958 c         4832 g         6371 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaatta
//
LOCUS       DARDHD010000026        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000026 DARDHD010000000
VERSION     DARDHD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.026"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SDA06_000814"
     CDS             367..555
                     /locus_tag="SDA06_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228109.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SDA06_000815"
     CDS             545..805
                     /locus_tag="SDA06_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228110.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SDA06_000816"
     CDS             846..1073
                     /locus_tag="SDA06_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228111.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SDA06_000817"
     CDS             1138..1710
                     /locus_tag="SDA06_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228112.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SDA06_000818"
     CDS             1711..2022
                     /locus_tag="SDA06_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4228113.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SDA06_000819"
     CDS             2013..2273
                     /locus_tag="SDA06_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228114.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SDA06_000820"
     CDS             2434..2793
                     /locus_tag="SDA06_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228115.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SDA06_000821"
     CDS             2790..4433
                     /locus_tag="SDA06_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4228116.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SDA06_000822"
     CDS             4468..5550
                     /locus_tag="SDA06_000822"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228117.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SDA06_000823"
     CDS             5594..6766
                     /locus_tag="SDA06_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4228118.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SDA06_000824"
     CDS             6753..7457
                     /locus_tag="SDA06_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4228119.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SDA06_000825"
     CDS             7462..8622
                     /locus_tag="SDA06_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4228120.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SDA06_000826"
     CDS             8698..8976
                     /locus_tag="SDA06_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4228121.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SDA06_000827"
     CDS             8957..9328
                     /locus_tag="SDA06_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG4228122.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SDA06_000828"
     CDS             9344..9748
                     /locus_tag="SDA06_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228123.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SDA06_000829"
     CDS             9745..10155
                     /locus_tag="SDA06_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228124.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SDA06_000830"
     CDS             10216..10803
                     /locus_tag="SDA06_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4228125.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SDA06_000831"
     CDS             10876..11343
                     /locus_tag="SDA06_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG4228126.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SDA06_000832"
     CDS             11389..11697
                     /locus_tag="SDA06_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228127.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SDA06_000833"
     CDS             11892..18626
                     /locus_tag="SDA06_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4228128.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SDA06_000834"
     CDS             18630..19502
                     /locus_tag="SDA06_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG4228129.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SDA06_000835"
     CDS             19499..20548
                     /locus_tag="SDA06_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228130.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SDA06_000836"
     CDS             20552..21376
                     /locus_tag="SDA06_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228131.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SDA06_000837"
     CDS             21394..>22031
                     /locus_tag="SDA06_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG4228132.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARDHD010000027        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000027 DARDHD010000000
VERSION     DARDHD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.027"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDA06_000838"
     CDS             <1..395
                     /locus_tag="SDA06_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228133.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SDA06_000839"
     CDS             398..1147
                     /locus_tag="SDA06_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228134.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SDA06_000840"
     CDS             1159..1428
                     /locus_tag="SDA06_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228135.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SDA06_000841"
     CDS             1433..1597
                     /locus_tag="SDA06_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228136.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SDA06_000842"
     CDS             1590..1892
                     /locus_tag="SDA06_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228137.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SDA06_000843"
     CDS             1889..2050
                     /locus_tag="SDA06_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4228138.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SDA06_000844"
     CDS             2047..2352
                     /locus_tag="SDA06_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228139.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SDA06_000845"
     CDS             2352..2708
                     /locus_tag="SDA06_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG4228140.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SDA06_000846"
     CDS             2695..2913
                     /locus_tag="SDA06_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228141.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SDA06_000847"
     CDS             2910..3329
                     /locus_tag="SDA06_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4228142.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SDA06_000848"
     CDS             3326..3883
                     /locus_tag="SDA06_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4228143.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SDA06_000849"
     CDS             3880..4176
                     /locus_tag="SDA06_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228144.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SDA06_000850"
     CDS             4253..4666
                     /locus_tag="SDA06_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4228145.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SDA06_000851"
     CDS             5012..5386
                     /locus_tag="SDA06_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228146.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SDA06_000852"
     CDS             5423..5590
                     /locus_tag="SDA06_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228147.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SDA06_000853"
     CDS             5616..5960
                     /locus_tag="SDA06_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4228148.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SDA06_000854"
     CDS             5965..6246
                     /locus_tag="SDA06_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228149.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SDA06_000855"
     CDS             6349..6663
                     /locus_tag="SDA06_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG4228150.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SDA06_000856"
     CDS             6641..8335
                     /locus_tag="SDA06_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4228151.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SDA06_000857"
     CDS             8355..9533
                     /locus_tag="SDA06_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4228152.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SDA06_000858"
     CDS             9496..10182
                     /locus_tag="SDA06_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4228153.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SDA06_000859"
     CDS             10182..11342
                     /locus_tag="SDA06_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4228154.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SDA06_000860"
     CDS             11352..12227
                     /locus_tag="SDA06_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228155.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SDA06_000861"
     CDS             12224..12535
                     /locus_tag="SDA06_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228156.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SDA06_000862"
     CDS             12525..12878
                     /locus_tag="SDA06_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4228157.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SDA06_000863"
     CDS             12868..13269
                     /locus_tag="SDA06_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228158.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SDA06_000864"
     CDS             13262..13666
                     /locus_tag="SDA06_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228159.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SDA06_000865"
     CDS             13678..14286
                     /locus_tag="SDA06_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4228160.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SDA06_000866"
     CDS             14306..14668
                     /locus_tag="SDA06_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228161.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SDA06_000867"
     CDS             14671..14853
                     /locus_tag="SDA06_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228162.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SDA06_000868"
     CDS             14870..18289
                     /locus_tag="SDA06_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4228163.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SDA06_000869"
     CDS             18340..19077
                     /locus_tag="SDA06_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4228164.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SDA06_000870"
     CDS             19087..21378
                     /locus_tag="SDA06_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4228165.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SDA06_000871"
     CDS             21402..>21988
                     /locus_tag="SDA06_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228166.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARDHD010000028        21901 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000028 DARDHD010000000
VERSION     DARDHD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.028"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..245)
                     /locus_tag="SDA06_000872"
     CDS             complement(93..245)
                     /locus_tag="SDA06_000872"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4228167.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(436..1314)
                     /locus_tag="SDA06_000873"
     CDS             complement(436..1314)
                     /locus_tag="SDA06_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4228168.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1353..2471)
                     /locus_tag="SDA06_000874"
     CDS             complement(1353..2471)
                     /locus_tag="SDA06_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4228169.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2468..4567)
                     /locus_tag="SDA06_000875"
     CDS             complement(2468..4567)
                     /locus_tag="SDA06_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG4228170.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4618..5439)
                     /locus_tag="SDA06_000876"
     CDS             complement(4618..5439)
                     /locus_tag="SDA06_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4228171.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5436..6245)
                     /locus_tag="SDA06_000877"
     CDS             complement(5436..6245)
                     /locus_tag="SDA06_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228172.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6266..6760)
                     /locus_tag="SDA06_000878"
     CDS             complement(6266..6760)
                     /locus_tag="SDA06_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4228173.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6772..7260)
                     /locus_tag="SDA06_000879"
     CDS             complement(6772..7260)
                     /locus_tag="SDA06_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4228174.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7337..10033)
                     /locus_tag="SDA06_000880"
     CDS             complement(7337..10033)
                     /locus_tag="SDA06_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4228175.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10461..11534)
                     /locus_tag="SDA06_000881"
     CDS             complement(10461..11534)
                     /locus_tag="SDA06_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4228176.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11531..12358)
                     /locus_tag="SDA06_000882"
     CDS             complement(11531..12358)
                     /locus_tag="SDA06_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228177.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12359..13165)
                     /locus_tag="SDA06_000883"
     CDS             complement(12359..13165)
                     /locus_tag="SDA06_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228178.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13177..14262)
                     /locus_tag="SDA06_000884"
     CDS             complement(13177..14262)
                     /locus_tag="SDA06_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228179.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14279..14821)
                     /locus_tag="SDA06_000885"
     CDS             complement(14279..14821)
                     /locus_tag="SDA06_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4228180.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15079..16068
                     /locus_tag="SDA06_000886"
     CDS             15079..16068
                     /locus_tag="SDA06_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4228181.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16065..16802
                     /locus_tag="SDA06_000887"
     CDS             16065..16802
                     /locus_tag="SDA06_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG4228182.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16799..17350
                     /gene="coaC"
                     /locus_tag="SDA06_000888"
     CDS             16799..17350
                     /gene="coaC"
                     /locus_tag="SDA06_000888"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG4228183.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17366..17998
                     /locus_tag="SDA06_000889"
     CDS             17366..17998
                     /locus_tag="SDA06_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4228184.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18083..18730
                     /locus_tag="SDA06_000890"
     CDS             18083..18730
                     /locus_tag="SDA06_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4228185.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18735..19718
                     /locus_tag="SDA06_000891"
     CDS             18735..19718
                     /locus_tag="SDA06_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG4228186.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20121..21635)
                     /locus_tag="SDA06_000892"
     CDS             complement(20121..21635)
                     /locus_tag="SDA06_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG4228187.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         6597 a         4745 c         3788 g         6771 t
ORIGIN      
        1 tagaaacttt cttttttttt cttgctatct ttttataatc acaactctct ataatagaat
       61 tacgatttat tttatacggc tgaaaaaatc tatcaccaaa aaaattcaaa cattaagcca
      121 aggattatga agtcaacatt tcgaaaaatg aacatacatt ggtaagcaac tataactatt
      181 accacagaca taaagtaaca caatatatta ctatttaagt gtacgccacc agtgaatatc
      241 ctcaaggtat acagcgcaca acaaaaagaa aaaaggaaac gcagaagcat ttcctttcag
      301 tgatttaatg aaaacaacac gatttgactt agagcctttt tatttgaatt cattttcgct
      361 ttatccaact tcatttgatc atttgattac tcggttaaat acttttgaca tgagtatcaa
      421 tgaaacattc attaattatg gttgcttatt tcttaccgct tttctggcca atgctaatcc
      481 gccgattatt ccggcattat cacctaattc gggcgtaata atgtagctat caattggtgg
      541 tatttcaaca tatccattta taagctcaac aaatttatcc tgaacttttt tcttcatgtg
      601 ttcttgtttc attacaccac cgccaaaaat aattctatct ggcgaaaaca tcagtgttgt
      661 attgtatgca cattgggcaa ggtaaaaggc ttcaatctgc caaaacgaat cgtcagccaa
      721 taaattctgg ccttttacac ccaaccgttt ttcaatcgct ggtcctgctg ccatcccctc
      781 taaacaatct tggtggaagg gacagtttcc tgaaaaattg tcctttggat gtcgcttgat
      841 caacatatgc cccatttccg gatgactgaa cccttccacg aaagtcccat tgaacaaggc
      901 accacctccg atacctgttc ccacagtgaa gtatgcaaca cctgctaacc ctttgccaga
      961 accagcaaca tattcaccat agcaagccgc attgacatct gttgtccatt caatcggaat
     1021 attgaaatat tgctttagcg ttcctacaaa attgaaattt tgccaagcaa gttttggtgt
     1081 tgatgtgata tgaccgaaac tcgttgattt gcattgaata tcaattggac caaagcagcc
     1141 gaccccgatg cctgccaatt gttccttaaa aggtttaaaa aaatcaatga cctgcttcat
     1201 tgtttcaact ggagttgttg tgggaaaact gactctttcc aaaatcgtta attcctcagt
     1261 accgactgca cagacaaatt tagtccctcc agcttcaatt gccccatata tcatcatagg
     1321 ttcctccctt cgaatatgtg aaaaacactt cattaacgcg gcaagcgttc tcgatcagtg
     1381 aaatgacgtt tagtatttcc tgaagcttct gttgaatacc agtaagcgac actagcaaca
     1441 tcgtcttgcc gctcaaataa gcgaacatca tcgttgccga tttgttgtaa agtgaccgat
     1501 aaatcttcgg taaaacagat ggggtcttga agatgccaac gataaagacc atgtcgtggc
     1561 attgcgtcgt caccaaaggc atgaacggca ttcaattttc ccgtttcaaa acgatctctt
     1621 gtggcatctc tatttgattg gtaaggatac cctaaaaagg ctgttgaata gtttttcgca
     1681 tgtggacgtc cgaactcatc gtactcatga aaggcccacg ccccgccaaa ataatcttct
     1741 gaacctgttg aggttacggt cggatactca ctatccccat caatgtagaa tttaaactcg
     1801 ccttcgcccc accaataacg ttctaggcag gtaagcgcaa ggtaagtacc cacgtaatag
     1861 ccttgaccct ttaatcgatc aattaactta taatcctcct gtaatttggt aatgcgttcc
     1921 cgatgccaat ttgcgtgaaa atataggcta ttttcttcca attcgtccac caaagcgtaa
     1981 tttatcgtat aaaaaaatga ggacatatcc ttcggatgta aattctcgat cgtaatttta
     2041 gcggatgttt ggaaaggcat ttcgaaatag gaattaaagc cccctgtagg attaaccact
     2101 atcggcaacg agttcacctc gcaacgctct ccaaacccat tacaaaagaa atcccctaaa
     2161 ggactttcaa cagcagggct atccgaccca tcccaataaa ttcttaagat taaatcacgt
     2221 aaaacaaaac tgccaaattc tgttcgatca cgaatcgtca tccacatatg acgaattatt
     2281 cccgttccag taatatccgc aatcacagtt tcttcaccag aagtcagcgg aattgcgcca
     2341 cgacctttgc ggcttggtcc tagctcgctt ggcaacatcg cagctttacc tttctcgcct
     2401 gttggatttt cgggagtgat tgctcgactc tggatttttt taaattgggt aatatttttt
     2461 agtatactca tctttttctc ctttgaatgt gaatcgtttt tcccttttct acgtgaagaa
     2521 tttttttcgt atcacttatt ttcaatggct tttctgacaa atttgaaata ctcaactcgg
     2581 attgcgtaca ttcaatcgaa atgaaggctt gaccgaactt caaattcttc caagaaaacg
     2641 ttaaattatc aacaggatca atttgcaata ctcctttttt gaatttcaat ccgaaatatt
     2701 gactgatcat taggctgaca aaaagacccg atgcccagcc gcatttgccg cagccaaagt
     2761 ctcgcacgac ttctccttgt tgagcctcta agcgatacgg ccaccaccac cacgaaccgt
     2821 ctaaatctgc caaacaaatt agttcttcta atcgggttac gaaagcttga gggttgtgca
     2881 gctcgctcat taaaacagtg ataaagccag gaaaagtggc gccggattct aatccccaag
     2941 tgatgccttt gatttcctcg ctataggttg gattgaaaga actagcagaa aaggccatgg
     3001 tgttctgata taaatgatct ttattatcta aaaattgata aaaaggcatc aacgtagtgt
     3061 ctgattcttc tccatcatgc atcaataaat taatttttcc atccacaatc acatcactta
     3121 aaaagattaa cccttgctca agaatcggtt tttgatagtg cttcactgaa taggttgttt
     3181 tctcctcatc accgattccc tccaaaaatt gctcaccaaa aggaccatcg atagtcattt
     3241 gtttacaaat tgattccttt agtttacagg caatgttttt gtatcgcttg ctaagactca
     3301 gttgttctag ggcttcaaaa atttctgcaa gtccaataca ggccttccaa agacaaagat
     3361 tgctgccggt atgatacttt ccaagtgcaa aggcgtctga taaccaaaca gatggaaaaa
     3421 gcattggttc ccctgaccga cgttgagcta atatgtcctc aataattctt gaagcattca
     3481 gtagaacctg cggacaccgc tcaaagaatt ttgtatcccc agtatgttca aaataaagac
     3541 tagccagtaa cgcactggat aacgaattac tcagagaatg attgatacct cctgtaaatt
     3601 ttgaaccagg aaacttaacg ccatattgat caaaccatag aaccgttttt tgacataact
     3661 ccggttcaaa catggtgaat ggcaatgatg cgtaatacat atccttattc caaattcgat
     3721 tagtagcagg atagctgccc caattgctgc cgacgacctg tccctgtcga ttcataccga
     3781 aactagaaaa gctttgataa agtgcacgac gatagagatg gacaactcta tcgtttggca
     3841 ttgacagctg cccaaatagc tgagcaaaat agttcaatgt ttccttcagc catttttcga
     3901 tatcagattc ctgaaaagtc attacttctt caaaagcatt tgggtccaca aaagcaatgg
     3961 cgaagcttgc cacttcctgt tgtgcgatcg tgtccttatt tcctttttga gaaatcaaca
     4021 cattttgctg atccgaatac ttctcttgat acgaaggcac atctttcact gaaacttgaa
     4081 ggctttcgtg tgtttcattc acaatgtaaa cctgttgaac aagcatactt aaacgcttat
     4141 tagccagtat gggagcaaag gatactacaa tgaccttaaa cgtcgaataa tgatgaatat
     4201 atctaggcaa acagtcttga atcacatcga cttcaatacg atgatccgat tctgacaaat
     4261 ggtagacaat cccgtttact tcaaccttta aggataacga attggtttga gagagctgtt
     4321 tattgaccca aacacctggt ttttcctcag ttagtaaatt ttctcgataa aaggtcaacg
     4381 tttgaatcgt cccgcaaaca tcaaggtttg ctaatgtctt ttgattactt acatcgaagg
     4441 tcaatccctt tacatcagga atattccgat agtagctgta ttcgtttgaa gtagtcacat
     4501 ttccgtcgtt taactcagca ttaaaaaatg ggtgtttggg tgaataaatt acttcattaa
     4561 tagtcatctt ttccctcctt gctaaaaaaa ctgcagccta gtagctgcag cttcctacta
     4621 tgcgattcca agtaatgaca ataaaattcc caagacgacc attccaaaaa taattttgat
     4681 gacgccgact tttttatcta gtaatttgaa acagctcaat gttaccaata acggtacaat
     4741 tcctttaaaa atactatcga ggatttcttg gacattcatt agttcttcgc ccttcattga
     4801 aaagctcaaa acggtattga atttgaccat actgatcgtc attgcaccta ccatgatcag
     4861 acccaagata ctagcagctt ttgtcagaat ttcaattaac ccgttcgcat acagcttttc
     4921 aatatattta gagcctaatg aatagcctaa aacgccgcca tagtatcgca tcaagtaatt
     4981 aggtatattg aacataacta aaaagataat tggtgccaaa atatttccgg cattggctaa
     5041 gttgatcgct agtcccgcgg taacgacacg ccaaactccc cagaacaatg aatcgccaat
     5101 tccagccaat ggtcccatca aactcgtttt gatcgcgttg atactttcca cgtcaaaacc
     5161 agctttttca gaattttcct tctccatcga tgccgcaatc cccatgacaa aggacgcgca
     5221 aggaatggtc gtcgaaaaat actgtgtatg acgttccagc gccgcagctc gatcttcttc
     5281 acttttgtag aaatgattaa tgaagggcat catagaatag gcgaaaccac cgccgcccat
     5341 cgtcaccgga ttcaccgaag catacatagt catcgagcgc caaaacatct tccgaaccaa
     5401 ttttttctca tctttagaaa ttccatcagc taatttcatt gaaagaaatc ctcctcttct
     5461 tggtcaagcg ttgcatttac agcagcatcg ctttgaactg catgaacaga tatatttttt
     5521 agttgtagat ctctttgggc tataacaatt gcaataatcg ttccaactgc ggcaattgca
     5581 atcaatggta actcgagata cgccataaca acggccccaa agaaaaacca cacagcaata
     5641 tttttactcc aaagcatccg taacagcatc gccatcccaa ctgccggcag cagatttcca
     5701 gcgactgtta atccgttcat gatgacctct ggaatattat ccaagacgct tttgacaaca
     5761 tctgctccaa ataaaacggc aaagaatgga ataagagcat aaagcgcata gtggacaaac
     5821 cacataaaga agtgcagatt gatgattcct ttttgatcac cagattttgc aaatttgaaa
     5881 aatttagcag ccatcggtcc acatacaaaa gaaaaaatca tattcattaa ctgtaatcct
     5941 aatactgcta gtggaaagac aatgggaata atcgcatcca cgtttcctcc cattttgatt
     6001 gcgaacgctg tagccagccc agcagccaaa gaaggctccg ccgtactaga gattccgata
     6061 ttgacgacac ccataaagat ggcttctagt gacgccccga caatcaaccc cgtctgcaaa
     6121 tcccccatca acgctcctgc taacggagca acaacaatcg gacgactcag catggacatc
     6181 ccgaacattt catgtccagc aattccaata aaaacggcca aagcgactac aaatgcatca
     6241 tacataattt ttctccttcc tttttctaaa aatcactcaa aatagagata gctgaacgtt
     6301 tttccatcgc cggtgtaggc tgaaaaacgg tatctggata aagttcaact aattctttta
     6361 agtcttgaat ttctgacgtc gttaacatca cttcctttga taaggttttc ttatcttccg
     6421 atggcggtgt cattttccca gcattcccca cgttcattaa ttcgatttcc gttgggaaag
     6481 ctttcgcaac ttttaccgca tctttgacat tagggactaa aaccatcaag cgcatgtcct
     6541 tcgcccgcgg atcttttaac aaatgaatcg cgtcatcaac tgttttaata atgactttga
     6601 cattcgatgg tgcagccatc ttcaatgtca tcttttgaat atcgttggta gccgcctcat
     6661 cgcttgctac tactaacccg gttaaattta gactctttgt ccaagtcatc gctacttgtc
     6721 catgaattaa tcgttcatca attcgcactg ctttaatcat ttttcttcct cctaaaaaaa
     6781 atcttcctct tcatttacta catgtacctc atcaagaatg gtcatcttta attcctgttg
     6841 gcatttattt accagttcct gaatatcctc ctttgttaat tccttggttg ccaatactat
     6901 ttcgagcaga attggcagat taaagccaga caaaatagtt acagcaatct ctttctccaa
     6961 aaaggttttg gtaatcttct gattgacact tccgccaaat aaatcggtaa aaacaaaaat
     7021 ctgatcctgc tcgttcacct ggtttacaaa tacattcaac tcctgttcaa aatcacactc
     7081 atccacataa gcattaatca ccgtaatctc ttgaaccttc cccgtcagta attgaatact
     7141 gctaagaaag cctgaagcta agtgaccgtg agtcgctatt agaattttct tcatatggtt
     7201 aattcctttc tttctgttca cttttatata agcaattcct gtgccaactt ttaaaaacat
     7261 tggtttaatc acgttaattc acattaaaaa aagtgtctaa cctaaaaaaa acagattaga
     7321 cactttttta gacactttat ttttgaatat acggtcctaa taattcatag agcagcgaaa
     7381 gctcataagc attgacttta aaattatatt tcatctccat tgattgaaaa atacttctag
     7441 aaacataata aaattcatct tcatctggtt tcaaatcata attcggttct tcagattttt
     7501 ctgtccgagc caagaataaa cgttccatca tcaatgccgt atgcatatag agatttagtt
     7561 ttacacggcc atttaaagtg aaatgatagt aattttcata tttagaaatg actgtttcca
     7621 cttcactcat cacgatcgtt gggttcagaa agttcaaacg gtccgcaatt ccttccaacg
     7681 aaaagaattt tactagctcc tgaatcaagc ggttaaattc tgccggcttc atttcaggac
     7741 tcaaaatatg ctccaaattg gctgctccat caccgtctaa gatgtcataa acattaatat
     7801 taggaattga aaaggaacgt ggaagatccg aagtcgtgat gaccaatttt gtcttgtcaa
     7861 aataagtttc atcattttgg tcgattaatt cacgtagttc tctgtagtcc ttcgtgaaaa
     7921 cttcgatgga ttcatccgat aaatgatgac gaaaaacatc tttcaatttg tccgaaatcc
     7981 ccaaacccga catacaggaa atgataatgt tgctactttg agaaaagcct tcaaagtatt
     8041 gaacattgat tgaatacttg tctgctgcat cctccgcaat ctgtttaaat ggcgcattca
     8101 tcgtcatctt caagccaata tcaagtgcaa tggaagtcgt caaattattc agaagaagta
     8161 actccccttt taaatcgttc ttgatctgtg tgtaaatttg atttaaagag cccatatcta
     8221 cgatcagcac tagactatct gtattcactt gccgtttcac gaaattcctt gtcgattcga
     8281 caatctctga cacattactc tctactggca tatccaacgc ttcaaaaaca taattgccgc
     8341 ataattgatt cactactgct tgaatgctgc tagccgtact atctccgtga gcaagcaaca
     8401 ggcccttcag ctcgatggat tcatctacat aatcagataa agtcacgaca ataagcagtc
     8461 ttagaaagtt ctcgtccttt tctgagagcg gtggtaaatg ctccaagaac tgattacata
     8521 agtaagtcgt ccgaggtaga tttgcagcta atgtacgatt tatcccggtt aagtctgtct
     8581 tatagcttgg ggaaatgaca taggatctgc taataatttc tgcaacaggt ccactcgtcg
     8641 tcaatccgta cgtttttcct aaaacggata cccagacctt tctaaacatc tgaatcatca
     8701 attttctact cattgataaa aaagaatcct tatcaatgtg atgcaccaaa tgctgtactt
     8761 cattctttat cgaagaaaaa ttttcattga aattaccttc atttatcagc tgagtaattt
     8821 tttccaaaat cgaatcataa tcaaacggtt tgattcgatc cgtttttatc ttttgatttt
     8881 tctttatcaa taaactagca atcggcaagc tttctacttc ggattccagc atatttaact
     8941 gaatcaagat ctcatttttc ccaatcgtta atggttcttc ttgtgttcga ctatacacat
     9001 ccgcacaact gattttaatg atattcttca gcttaccaat attcccacta aagggatgag
     9061 ataacattaa agacaaggct tctcttgaaa caataatatc tttttgtaaa acatccgctt
     9121 cattttgaaa aaataattga atcagctcta atcgttctcg aatggggcga tcttcaaaag
     9181 ttggcaacat aacactaatt tgaatacgcc gatcaaaggt gtctaataaa taattttcac
     9241 ctttttccgt cgtggcgaaa ataaaacgaa ccttagcaga atgccaattt acattttcac
     9301 cgactggacg atagtttcca gtatcaataa atagaaagag cttttcttga ttttctttcg
     9361 ataggcgatg gacctcatcg agaaaaagat agccgttgtt ggccgttgcc aataaaccat
     9421 cggtatcctt ttcagccccc gtaaaggccc ccttcttgta tccaaacagt gttgccgaaa
     9481 gcagttcggg attgtccgca taatcggcac agtttaagat agcaaatggt gcatcttttt
     9541 caataatttg ctcatggata gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa
     9601 cacccgtggc tccatttatt aatacactga gtccgttagg cggatatttg acagcggcta
     9661 tacatttttg aatgacctct ctcagacttc cgtcatgacc aactacagaa tgaaaggctt
     9721 cgtttatttg ctccgtatta tctacaaccg catattcatc taaaggcttc cacaaaacag
     9781 gctttcccgg taatttctcg acctttttat cttccactaa tctcgtcaaa taatggctgg
     9841 cattctgacg agagatacct agattcttcg aaattttctc ggcagtgaca gaagacatat
     9901 tccctgttag tcgtatcagc tcatcaaaaa ctcgatcttt agcgctcttt ttcatttttt
     9961 cctccagttt aatgtgtcta aaagcataat aaactatcgg ctgcaatatg tctattctat
    10021 atttgtattc caatttttac tttgaaacac aaatagtcga atatttgcct cagatatctg
    10081 acaaaataat ttatctacca atttagtttt tatctaatat gatttttatg ttttttcata
    10141 gctacaaata ttgaactgga ttcatatatt agttttatta atagattaca gaagggttaa
    10201 gtatatttgc atagtttcca attactttat ccttctgtaa ctttttcaat cttagattta
    10261 ctagcccgtc tcgggcccta ttttacaatg gttttgtcca cttttctctg gtagaaaaac
    10321 gtggtagaaa aaataaaata acacaaaaaa agcggcgttt catccgcttt tggtagaaga
    10381 ttggaaaact cagatagaaa aaggacgatt tttagctctt tagaacgttg ataagtcccc
    10441 attcttactg agttttgcca ttatctccta tacatcttga attctaagaa taggtcatta
    10501 tagatgccta ggtatttcga tcctagatct ttgtagactt caagatggga gatcgtgtca
    10561 tcagatggat agaattgttc gtccgacgaa atgtcttttg gcaataacgc ttttgcttct
    10621 tcatttggtg tagaataacc aatatactct gcattttgtg ccgcattttc cggttctagc
    10681 ataaagttga tgaaatcata ggcaccttct ttatttttca cggtttttgg cataacgata
    10741 ttatcaaacc aaagattgga tccttctggt gggatcacat aatgtaaatg ttcattttcc
    10801 cacatcatat cggcagcttc gccagaaaat gtcactgcca cggaggcttc ttcattgatc
    10861 atatacattt tgatctcatc tgccacgatt gctttgacat ttggtgttaa atgattcaat
    10921 ttatttgctg cttctgtcaa ctgtgccata ttcttactat tcaaagaata gcctaaacta
    10981 ttcaaagaaa gccccataac ttctcgtgca ccgtcaatca gcatcaaact gtcttttaat
    11041 tccggcttcc acaaatcatc ccaatgctta atttgatttg catcaaagaa tttatcgtta
    11101 tatacgatcc ctaaagttcc ccaaaaatat ggaatcgaat actgattatc tggatcaaaa
    11161 tccaagtcta aaaatcgtgg atcaatatta tttaatcctt tgatcttcga atgatctaat
    11221 ggaagtacta gtttttcttt gatcattttt tggatcatat attcacttgg tatagcgatg
    11281 tcgtagtttg tcccaccttg ctggattttt gtgaacatcg cttcattaga atcaaatgtt
    11341 tcataattca ctttgtatcc atattctttt tcaaatttcg tgatcaaact aggatcgata
    11401 taatctcccc agttatagat ggtaagtacc tttgctccac tcatgccact tgttttttca
    11461 agttgacgaa caccaagcag cagcaccgca ataatgatga gaatacctaa aaacagagat
    11521 tgtaattttt tcatcgcagc cgtgccaccc cctcgcgtac tcttctgttt ttcttcagcc
    11581 gttttggacg gttttcttta ctaataaagt agtatccaat gaccaatatc atggagaaca
    11641 agaaaactaa cgcacttagc gcattgattt ctaaactgat tccttgtctt gcacgagaat
    11701 agatctctac tgagagcgtc gtaaagccat ttcctgtgac gaaaaatgta accgcaaaat
    11761 catcaagaga ataggtaaat gccataaaat atccagctat gattcctgga gaaagataag
    11821 gcaaaatgat atttttgact acttggaaat tattagcacc aaggtctcta gccgcatcga
    11881 ccattgaatc gttcatttcc tgcaattttg gcaagaccat caagacgacg attggaatgc
    11941 tgaaagcaat atgggaaagc aatacactct gaaaacctaa tccgaatcct agtagtgtaa
    12001 agaaaatcaa gaaactagca ccgataataa catcaggaga gactaataag atgttattca
    12061 tgctcaacaa cgctgtccgt gcacgtcgtt ttttcgtgta atagattccc atagccccaa
    12121 atgtcccgat gatcgtggcg atcaatgcag ataagaaagc cagcatgaac gtattcaata
    12181 cgataatgat caaacgtgta tcttcaaaaa cagcttcata attttcccaa gtaaatcctg
    12241 taaagctgtt catcgttcct ccttcattga aggaataaaa aatcagatag aagatcggtg
    12301 catataagaa taaaaagacg atccctaaat aaagatacga ccatttaaat tttttcattt
    12361 atgagcgacc tccttttttc ttctcgcctg tcaggagcat cacgatgaac atagcaatga
    12421 ttaaaatcac accgattgta gaacccatcc cccagttttg tgtaaccaag aaatgctgtt
    12481 cgatagccgt tcctaaagtg atcacccgat ttccgccaat cagacgagtc agcatgaata
    12541 aagaaagaga aggaataaat actgcttgga caccactttt cacaccattt aatgataacg
    12601 gaaaaatcac tcgtcggaag gtctcaaagc tattcgcgcc taaatctcta ctagcactga
    12661 tcaaagaagg attcatctct tccaatgcat tgaaaattgg cataatcata aatggaatct
    12721 cgatatacgt tgccacgaac agaaaactga agtctgtgaa taatatctgt ttcggcccga
    12781 tcccgataaa atcaaagaaa tgattcacgc tgccgtgcac actgaaaatc ccaataaatg
    12841 cgtaagcttt aagcagcaga ttcacccatg taggcaaaat aatcagcatc agccataatt
    12901 gcttgtgctt cagttttgtt aaaaaataag cagtcgggta actgatgatc agcgtgaaaa
    12961 acgtgatcaa aaatgcatac catacagaat tgatcgtcat agaaagcgat gtccccgacg
    13021 taaaataaga tagataatta ttcagcgtga attgcccatt catatcaaaa aaagattgat
    13081 aaatgatcat taatacaggc gcgatcacga ataaaccgag ccacaaaata taagggacgg
    13141 aatagatcct gcgcattgtt ttcattcttg tcccccctat tcttcgtagc tgtcaagacg
    13201 tgcatcgaat tcttcttcag actcgttgaa acgcatgaca tggatatctt ctggctcaaa
    13261 ggccaatcct actttgcttc cttctttagc ttttttcgtt gaatggacca tccattcatt
    13321 ttgctgttca tcataacaga tgatttcata atggacacca cgaaacagct gagtatcgac
    13381 cgttacgact agttttcctt tatctggtgt cgtgatcgtc aaatcctctg ggcgtaaaac
    13441 aacttcaact ggttcattta gacgcatccc gccatcgaca cattcaaatt gcttaccaac
    13501 aaactcaact agattatctt cgatcattac accgtccacg atattgctct ccccaacaaa
    13561 atcagcaacg aaatgattga ttggctcatc ataaatatct acgggtgtac cactttggac
    13621 gattttccct ttattcatga caaaaatctc atcactcatt gccaacgctt cttcttggtc
    13681 atgagtaaca aaaataaacg tgattcctaa tcgttgttgc agatcccgca gttcatactg
    13741 catatctgta cgtagtttca aatcaagcgc cgataacggt tcatctaata gcaatacttt
    13801 cggttcattt acaattgctc gtgcaatcgc tacacgctgt ctttgaccac cagacatttc
    13861 actaatctcc cgtttttcgt atccaggaag ctggaccata cgcaaagcat cttttacctt
    13921 cttttcgata tctgatttgg acattttctt gatcttcaat ccaaaagcaa cgttatcaaa
    13981 aacattcata tgaggaaaaa gtgcataatc ttggaagact gtatttactt gtcgcttatt
    14041 agctggcaaa tcattcaacc gtttcccttc aaaatagatg tccccttctg tagcttcggt
    14101 gaatccagct ataatacgta ggatcgttgt tttaccacag ccggaaggac caagcagtgt
    14161 ataaaatttt ccttgttcga tctcaaaact tacttttttt aatactgttt catcatcata
    14221 gcgtttcact acttgatcaa atgaaatgat tggtttcccc acgtttttac cccctatatt
    14281 acaaatatga ttgcgttgca acaatcaata cttttgttcg ttttttgaga tgattcctta
    14341 attgatgttg ttttgtcgct tgataataga tcgactgtcc tgcttttgcc acatagaccg
    14401 tctctcctaa gcttaactct aattcccctt ctaatacaaa aataaacgtt tcagataatg
    14461 aaggttcaaa cgtcttatat tctcctgcac cttcaaaagt cagcaagaca ggctccattt
    14521 ctttttcatt cgaatcagcc acgagccatt ccaattcata cccgttttct tcatcgaaat
    14581 aaaccgtatg atcttctttt gaataaacca cttgcagatt cgctgcttct tgatgaaaaa
    14641 attcttctgg agtcacacct aagacttcaa gaatagaaaa gaatgtttcc attgatggtg
    14701 aacttaaatc tcgttccaac tgggaaatat acccttttga caaatctgtg cgttcgccta
    14761 gttcttcttg tgtcaaattc ttttggatgc gcaaatttct taacttctca ccaatttcca
    14821 taatttcctc cgaatcgaag ttttctaata gtaaacttcc tgtttactac cactcgtatt
    14881 agtatacgaa atttattgaa aaaggcaacc ctttgtaaaa ttttttttgc tgaatatttc
    14941 tttatctttt ggaataaatt ctagttgttt gattctttat ttttcaccta atttttgtct
    15001 aaatttacgg aagaacaaga ataaaagagt aggctttatg gtatgataaa agaaaatttt
    15061 aaaaggagtg tctcacgaat gaacgctttt cgatcttttc aggtaataaa ggaacctgtt
    15121 ttccatacag caatcagtga tctgccattt actgagttgg ctgaaaatga agtcttggta
    15181 aaaattgcct attctgatgt caattataaa gatgcattgg caatgagcga gtctggtcag
    15241 gtgatacgaa catatccgat gactccaggg attgatttat ccggaactgt cgtccaaagt
    15301 aacaatccac gtttttcaaa agaagatctt gtacttgcta ctggttttgg gcttggcgtc
    15361 acccatccag gcggttatag ccaatatcaa aaagtaccag gagactggtt agttcctcta
    15421 ccaaaaaata tgacgctgcg tcaagcgatg atccttggta cagcagggtt tactgctatg
    15481 ttgtgtgtga acgctttaat ccgtcaaggt atgacctccg ataaaaaagt tgtcgttact
    15541 ggtgcttctg gcggtgtagg cagtacagcg atagccctgt tacataaact aggattccag
    15601 tcaatcattg ccttttccag aaaagaagag tctgtcacat ggctaaaaag tctaggtgct
    15661 ttgcaagtcg tacgacctga tgaatttctg ccagagacaa caaaagcttt aggaaaacag
    15721 caaatcgact atgtcatcga cacagtaggt ggcgagcagc taagcagtct tcttccattg
    15781 atctcttata acggtgcagt agctttatgt ggcaatgctg ggggaatcaa actaaacgcg
    15841 actgtcttgc cttttattct gcgaaacatc cagttgattg gaattgattc tgtcaatgta
    15901 ccaatagatc aacgtctttc tttatggcaa cagatggcgg atttgcagat tgcagatgaa
    15961 ctggtcgtcc aagagattac tctggatcaa ttgcctgaaa cagcatctaa actacttgct
    16021 ggaacacacc aaggaagaac actagtaaat gtaggtgacc ataaatgaag attctaatca
    16081 cagctggagg aacgactgaa aaaatcgatc aagtacgtgc tattacaaat cattcgacag
    16141 gacgattagg gcaagcatta gctgattatt tagctgccaa tccggatacc acaatcgatt
    16201 acgtgactac gaaacaagca ctgaaaccga aaagacactc caatataacg atttatacga
    16261 tcgaatctgc actggactta tttttacaat tagaagaact aacgaaaaaa gaacactacg
    16321 atgcgattat ccacagtatg gcagtcagtg attttactcc tgcttttagt ttttcagaag
    16381 aacagctggc aaaaaaccta cccacttctt caacgcaaga agaactggac aattggtttg
    16441 cggaaaatga acagacagat accactgttt caaagatctc ttctaacacc gagcatcttg
    16501 tgctcgtttt gaagaaaaca ccaaaaatca tttcttatct acgtgaatgg cagccaaaag
    16561 caaaaatcat tggtttcaaa ctattagtag atgtaccaaa ggaatctctt ctggcagtcg
    16621 caaaaaatag tctgataaat aacaaaactg attttatttt cgcaaatgat ttgacggaaa
    16681 tccatggcga gacacaccac ggctatctct tatctaaaga tggaacagtg gaagaagctc
    16741 aaagtaaatc tgagattgct gcattgatta ctgagaaaat tagactggag gaatcaaaat
    16801 gaaaaagatc ttactaggcg ttactggaag tatttctgct tacaaaagcg ctgatcttac
    16861 aagtcaattc gtcaaattag gtgcccaggt agatgtgatt atgtctaaaa gcagcacaca
    16921 ttttatcacg ccattaacct tgcaatctct gtcgaagcgc gcagtccata cagacgtcat
    16981 gcaagaatca aatccgcagg taatcaatca tatcgaacta gcaaaacaag ctgacttgtt
    17041 tctcatcgcg cctgctacag caaacattat cggcaagcta gcaaacggca ttgcagacga
    17101 tcttctttca acagtcgcaa tggcgcttcc agtgggaaca ccaaaattga ttgcccctgc
    17161 catgaacacg aatatgtatc aaaacccgat cactcagcgt aatctggaaa cattgagatc
    17221 tgtcggctat caggaaattg aaccaagaga atctctttta gcttgtggag attttggaaa
    17281 aggtgcctta gctgatattc agacgatcat cgaacaggtc atgctgactc ttaatgaaag
    17341 aaatgcttag aaaaaaggag tacttatgaa aaatacaaaa aactttacat taactgcgat
    17401 gtttttagct attttgattt tattagctgt tacgccttta ggttttatcc ctatcggtcc
    17461 aatcaatgca acaaccatgc atattcctgt aatcattgct tcgatcgttt taggaccaag
    17521 actaggtgct tttcttggcg gtacatttgg actgatcagc atgatccgta gcacagttat
    17581 ccaaacacct ttgtcttttg tcttttctcc gtttatccca gtcatcggaa cagaacatgg
    17641 aagctggaaa gccttgctga tcgcgtttat tcctcgcatc ttgattgggg tggttcctta
    17701 tttcgtttat aaaggtctgc gcaaactaat gaaacaaaaa gcagattccg tttctttatt
    17761 cttagctgga ctttccggtt cattgaccaa tacgatttta gtgatgaata tgatctactt
    17821 tttattccag caagattatg ccaaagtcat cggaacaaat ttaaatgctg tttattcagc
    17881 tattctagcg gttattttta caagtggtat acctgaagga atcgtcgcag gtcttgcgac
    17941 tgcagcagta tgcaatgtct tgttaagata cttcaagcac ccttctctta ctaactgata
    18001 gtcatcgtag ccgatctctg atttttttct cttttggtgt tactattgaa tggaaagatt
    18061 tgacgtcgaa aggatgatcc aaatgaaaat ttttgttgta ggtgcaaacg gacaaattgg
    18121 ccgacacctg ataaaagatt tagctccaag ttcccatgaa atttttgcag gggtgagaga
    18181 tgttgcttcc caaacattag taaaaaaaga gaatgtttcg tatgtctctt ttgatttaac
    18241 ttggtccgtg gaaaaaatga ccgaagcatt caaaggcatc gatgtattga tttttgctgc
    18301 cggctctcaa ggaaagaatc ttctgcaagt agacttggat ggcgcaatca aaacagtgat
    18361 cgcagctgaa aatgcgcatg tctctcgcta tctgatggtc agtgctgttt atgcagatga
    18421 accagcaaaa tggcctgagt ccatgacaga ttattatatt actaaacatt atgcagatga
    18481 atggctgaaa cgaactaact tagattttgt cattcttcaa ccagtcacat tgactaacga
    18541 tgaagaagtt acttccattc aattaactaa gcctaatgag aaggcatcaa aaacgatcac
    18601 tcgctctaca gttgctgctg tacttgccgc tctagtggaa gaaacggata tttctcgtac
    18661 gactctagta ctttctgaag gtagcaaaga gttgaataca gcattccaag aatgggcaaa
    18721 ggaggaatag atccatgcgt gccatttcaa tcaagcatcc tggttctgta gatgtattag
    18781 aacaaattga agtgcctgtt cctaaaccaa aacaaggcga attactgatc aaagtccata
    18841 cagcagctgt caatcgaaca gatattatga aaagagaatc agtctctttg aaacctccct
    18901 accctatttt gggagtcgaa gtagctggag aggtagttga gaataaaagt gatatctcca
    18961 cttttactcc tgggacaaaa gtatacggat tagtcaatct aggtggttat gccgaatatg
    19021 ctgtgatgcc tgccgatcgt gcaatccttc tccctgatac gctagactat gtttctgctg
    19081 ctggaatctc agaagttttt ttgactgctt atcaaacact ttactggctt ggaaaattac
    19141 aagaaggcga aactgtcttg atccatgcag gagcaagtgg tgtgggaaca gcagcgatcc
    19201 aattagctag acagctttct cacgcgaaaa tcatcgttac tgcaggatca gaagaaaaac
    19261 tcgccttttg taaagaactt ggtgcagatg aattgatcaa ctataaaaaa caagactttc
    19321 aagaagaggt acgaaaaatc actgaaggtc ggggagtcga tgttattttg gatttcatcg
    19381 gcgcttctta ctgggaaaag aatttagctt ctatagccgt tgatggacgt tgggtcttga
    19441 ttggtatgct aggtgggacc atcgtgcctg agatagacct tggtgtattg attgggaaac
    19501 gtgtccaact gcttggtaca ttattgacac ctagaagtga tcagtataaa gcagagctcg
    19561 cacaaacttt tatgcaaaaa gctgggcctt ttctagaaaa tggcaagatt cggccaatca
    19621 ttgaccgtac attccaattc agcgcagtca aacaagcgca tgaatatatg gaagcaaaca
    19681 aaaacatcgg aaaaatcatt ttgaagataa ctgaataaaa tcatgctgat caaaaaagag
    19741 gctgggacag aagcgtttaa ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca
    19801 aatttttgtg aatttcagct tatttccgaa ggaatttctt ctgcttccgc cgtttatacg
    19861 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt ttccgaataa
    19921 acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagaag
    19981 ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttca
    20041 ctaaacagtg ttcaatcagc tgaccctcgg tattagctca aaacgctgat ttcacaacct
    20101 ctttttattt acgttctatt tcattcattt tccgaagcaa atcgagctgc ttgtatgccc
    20161 gcttgtcgcc caaaaataat gatctccgca actgaatttc caccgatccg gttgtttcca
    20221 tgtaagccac ctgtgacttc accagcagca tataaccccc taattggctg gttttcttta
    20281 tctaatactt cagtgttcgt attgattttc acaccgccca tcgtgtaatg gattcctggt
    20341 gcgattttga tcgcataata attcggagac gataagtcat gttccatggc tgtggcacgg
    20401 ttgaattgag catctttttt ctccgcaaca tccgtattcc atccatcaac tgtttttgct
    20461 agtgtttctt ttggtaaatc gattttttct gctaattctt ccattgtctt tccagttgtg
    20521 acatatcctt tttggtcata aaattcaata gctgtggcac gatcacgcac gccttggtca
    20581 aaaatcaaat aagctgattt ttcaggcaat gctgtaattg ctgcagatac tttatccctt
    20641 gtatccattt cattgacaaa tcttttcccc tcttgatcga ccaagattgc tccttctccg
    20701 cgaacaacct caccaatcaa gaccccctca tcctgctgca cagttggatg aatttgtatt
    20761 ttatccatat caacggtttg tccacctaat ttttcaatca tggtgatacc atctccagtt
    20821 gatccttctt gatttgtcgt aatataatca gctaaatcgg gacgatattt ttcaataaat
    20881 tctttgctgg caccaaaacc gcctgtggta actacgattg cttttccggt gacctctttt
    20941 ggttcttctc cttgaacatg tacagtcact tcattgaccg tttccccatt tttcttaatg
    21001 tctgtcacat ctgcgttgac aaaaataggg atttcccgct catggacgtt attcaataac
    21061 ccctccacta agtatccacc aatagccgag ccatcagacg gacgatgtgt cctcttttca
    21121 ctcatccctc cagtgatcgt taagttgtcc agtttgatac cgttttgatc tagccaatcg
    21181 accgcatcag cagagtgatc gacaaagtat cgaagcattt ctttatcatt cgttcctcca
    21241 ccacctttta gcgtttcttc atagaaggca tcattcgagt ctttgattcc ttcttctttt
    21301 tgaaattttg tttctgaagc attcataccg cttgatgatt tagatgtgtt tcctccggca
    21361 acaggcatct tttccaaaat tactggattc aacccagcat cttttgcttc gatagctgct
    21421 gccatccctg caccaccagc tccaataatc acgacatcat aagattcttt catttctttt
    21481 ggatctgtat atacttgttc agaagcgcct gatgatactt cggcagattc cttcgtacta
    21541 gaacttgtct gcgctgtatt tttctgatct gcgccgcatg cgctcatagc aagaagtgca
    21601 acggctgtta ataaaccagc aactaatttt ttcatttgta tccccctttt ctccttatac
    21661 tatattaagt gatttttttc acaatatcaa ctctgttttt ctttaagtca tatagctttg
    21721 gtaaacacac gcttcttagt ataattaagg atatacatga aggagttatt ttatgaatca
    21781 tttgaaaacc ttttttacga acagtacaaa aactgcaggt agaatcacaa acggaatcta
    21841 tattttatta cgtatcatcg tcgttattat gctagtggat caggtctgga accaacactg
    21901 g
//
LOCUS       DARDHD010000029        21434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000029 DARDHD010000000
VERSION     DARDHD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.029"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SDA06_000893"
     CDS             complement(59..187)
                     /locus_tag="SDA06_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4228188.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDA06_000894"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDA06_000894"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4228189.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SDA06_000895"
     CDS             complement(1674..2729)
                     /locus_tag="SDA06_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4228190.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDA06_000896"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDA06_000896"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG4228191.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDA06_000897"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDA06_000897"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG4228192.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDA06_000898"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDA06_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG4228193.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDA06_000899"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDA06_000899"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG4228194.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDA06_000900"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDA06_000900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG4228195.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SDA06_000901"
     CDS             10491..12050
                     /locus_tag="SDA06_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG4228196.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SDA06_000902"
     CDS             complement(12161..14092)
                     /locus_tag="SDA06_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4228197.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDA06_000903"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDA06_000903"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4228198.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SDA06_000904"
     CDS             complement(15021..15773)
                     /locus_tag="SDA06_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4228199.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SDA06_000905"
     CDS             complement(15932..16141)
                     /locus_tag="SDA06_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228200.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SDA06_000906"
     CDS             complement(16152..17480)
                     /locus_tag="SDA06_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228201.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SDA06_000907"
     CDS             17616..17771
                     /locus_tag="SDA06_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228202.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SDA06_000908"
     CDS             complement(17845..18432)
                     /locus_tag="SDA06_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG4228203.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SDA06_000909"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SDA06_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG4228204.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SDA06_000910"
     CDS             complement(20012..21301)
                     /locus_tag="SDA06_000910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG4228205.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT         5657 a         4610 c         3822 g         7345 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat tttt
//
LOCUS       DARDHD010000030        21387 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000030 DARDHD010000000
VERSION     DARDHD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.030"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..1558
                     /locus_tag="SDA06_000911"
     CDS             86..1558
                     /locus_tag="SDA06_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228206.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            1641..2429
                     /locus_tag="SDA06_000912"
     CDS             1641..2429
                     /locus_tag="SDA06_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG4228207.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(2496..3164)
                     /locus_tag="SDA06_000913"
     CDS             complement(2496..3164)
                     /locus_tag="SDA06_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG4228208.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            3279..4991
                     /locus_tag="SDA06_000914"
     CDS             3279..4991
                     /locus_tag="SDA06_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228209.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4994..6763
                     /locus_tag="SDA06_000915"
     CDS             4994..6763
                     /locus_tag="SDA06_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228210.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(6968..7534)
                     /locus_tag="SDA06_000916"
     CDS             complement(6968..7534)
                     /locus_tag="SDA06_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4228211.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(7534..8490)
                     /locus_tag="SDA06_000917"
     CDS             complement(7534..8490)
                     /locus_tag="SDA06_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG4228212.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(8490..9146)
                     /locus_tag="SDA06_000918"
     CDS             complement(8490..9146)
                     /locus_tag="SDA06_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG4228213.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            9516..11534
                     /locus_tag="SDA06_000919"
     CDS             9516..11534
                     /locus_tag="SDA06_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4228214.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    11604..11810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11961..14375
                     /locus_tag="SDA06_000920"
     CDS             11961..14375
                     /locus_tag="SDA06_000920"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG4228215.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            14723..16276
                     /locus_tag="SDA06_000921"
     CDS             14723..16276
                     /locus_tag="SDA06_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4228216.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(16657..18441)
                     /locus_tag="SDA06_000922"
     CDS             complement(16657..18441)
                     /locus_tag="SDA06_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228217.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(18456..20177)
                     /locus_tag="SDA06_000923"
     CDS             complement(18456..20177)
                     /locus_tag="SDA06_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228218.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            20277..21038
                     /locus_tag="SDA06_000924"
     CDS             20277..21038
                     /locus_tag="SDA06_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4228219.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         6813 a         3844 c         4384 g         6346 t
ORIGIN      
        1 tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat ttttagatcc
       61 tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt tggtcacagt
      121 gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa caaccgctat
      181 gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat tttcgattta
      241 tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata aagtgggacg
      301 gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat tttgcggttt
      361 atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg gtgctggggc
      421 aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa agcgagcaaa
      481 agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac cgcttgcggg
      541 aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg tgccgatcgg
      601 aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca tacatgagtc
      661 taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct cattgctgtt
      721 agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt tgctgctagg
      781 tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag atcctgtcat
      841 catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg cagcattggc
      901 tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag gtgtattagg
      961 gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct ggatggtcgg
     1021 ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct tgagaattgg
     1081 actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca gaagtaacta
     1141 tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga ccatgactac
     1201 ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag caacttcttt
     1261 taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg gtttattgat
     1321 aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg cagatatcat
     1381 gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt tgttaccatt
     1441 aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt tactcgtgat
     1501 cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa aacagtagtt
     1561 tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa aaataacttc
     1621 ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg aatgtgttag
     1681 aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg attggcacca
     1741 gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga atgggtggca
     1801 atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac tacggattag
     1861 ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat gacatgaagt
     1921 attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc ccgcaattaa
     1981 caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat ggcgcgttaa
     2041 aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca ggcgctgttt
     2101 ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt tgggaaggga
     2161 tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac ttattgaaag
     2221 aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg gaatctgatt
     2281 ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc ttggcggtca
     2341 cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg attcaacgtg
     2401 cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata gctcctatag
     2461 agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc tcttctttta
     2521 ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag tgctttttca
     2581 ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt ttcaactaca
     2641 taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat aattttttgt
     2701 cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt gtggagagag
     2761 gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa ttggatcatt
     2821 tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc taattctgcc
     2881 ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag caactgttca
     2941 ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc agccattatt
     3001 tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg aacatgaatt
     3061 ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc caggatatca
     3121 aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc aacctcgtgt
     3181 attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac agtgttaaat
     3241 tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc ttacgacatg
     3301 cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc atggttgcag
     3361 cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt acagaagata
     3421 atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta ggattagccg
     3481 caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt gcagatatcc
     3541 gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa cagttctctg
     3601 ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat ttagtgatga
     3661 tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc attttagcta
     3721 tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg gtatttctga
     3781 ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa aaactgattg
     3841 atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg aaatcttttg
     3901 tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta acaaaacaca
     3961 acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg gctgccaatt
     4021 tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat cctacgttaa
     4081 ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca atcattattg
     4141 gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg attgcggaaa
     4201 ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag ttgacgggtt
     4261 ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat acgcttaaag
     4321 atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg actggtgccg
     4381 ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa ggcaaagttg
     4441 aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa acggtttcct
     4501 ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg cgccaaggga
     4561 aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa gcaaaagaat
     4621 tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc agtaactttt
     4681 ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat ccaaagatct
     4741 tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg gtccgagaag
     4801 cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa attgcttctg
     4861 ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc gaaggaaccc
     4921 atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact caaaaaggaa
     4981 cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc actatttgaa
     5041 gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta cctatctaca
     5101 agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt acgtaggagg
     5161 cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga aactcttgct
     5221 gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca cacaagtagt
     5281 agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa aattgacgat
     5341 ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca gtgacttaga
     5401 taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg ctgttttact
     5461 tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg ctacaattgc
     5521 ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac ggaaatatgt
     5581 ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa aaatcagcgg
     5641 acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat tcttagagca
     5701 taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc tactatttcc
     5761 gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg gcggctggtt
     5821 agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga cattcgtcca
     5881 gtattcacaa caatattacc aaccactgat gcagatttca tctggataca gcatgatcca
     5941 attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag atgaagtcaa
     6001 accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc agcacgtaga
     6061 ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg tggacaaagg
     6121 agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca tgaatctgct
     6181 aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag atatccgaga
     6241 ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat ctgtattgtt
     6301 ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag atgaagaagt
     6361 catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag aaaaaggtta
     6421 tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc agttgatcag
     6481 tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag caacaagtaa
     6541 tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa tcaaaggaag
     6601 aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata gaatcgttgt
     6661 cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag cagaaaatgg
     6721 attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt gtgaaaaagc
     6781 tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt ttttgatttg
     6841 gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag gttatgaaat
     6901 caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta tgttttgcgt
     6961 tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc ttttggttga
     7021 taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa aaagaatcaa
     7081 ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg atcaatcgtc
     7141 cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc tctggcatag
     7201 tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct gctttgagat
     7261 tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac aatgagacac
     7321 gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct tgttgctgga
     7381 tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa accgtatcgt
     7441 ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt tcttgcagta
     7501 atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt tccgtacatc
     7561 atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat tcaagacttc
     7621 ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat cccctgtcaa
     7681 acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga cattgacata
     7741 gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca ttctcgtttt
     7801 gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta aaatggtgcg
     7861 tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga tcaaatgcgt
     7921 acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag tttgataatc
     7981 atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc caagttctac
     8041 ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat ctggcagaca
     8101 atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta cttgctcaaa
     8161 tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga aataaacgat
     8221 gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct ggaattggat
     8281 tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac tgcaaaaagt
     8341 acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc catcgatcgg
     8401 caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag aatgatatct
     8461 tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta agagttatct
     8521 atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga aagactgagt
     8581 aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg gcttatcccg
     8641 acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt tcttttttga
     8701 acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc tgctgcatgt
     8761 ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg acgctctcgc
     8821 atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa tccagaaacg
     8881 aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc acaagcaaat
     8941 aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta gctgttcaga
     9001 tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga aagccacgac
     9061 agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa gctaccagcc
     9121 acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga cttattatag
     9181 catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga ttcctctttg
     9241 aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt ttggtaatgc
     9301 aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa caagaacgta
     9361 gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt acaatatgat
     9421 tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa gttataatta
     9481 ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca agaaaagaac
     9541 ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca gcaatcgtgg
     9601 gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt cctgttcaag
     9661 cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt atcacagaaa
     9721 ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca gtgatgatcg
     9781 cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca ccttattata
     9841 atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat acgcttgaat
     9901 ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct tcttttgctg
     9961 aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac ggttattact
    10021 atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct tgtttgacag
    10081 ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc gcaacttacg
    10141 gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg actacaggtg
    10201 gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt aatacaaact
    10261 caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt tggggaatct
    10321 caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc caaaacaatt
    10381 tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc tcttcgacga
    10441 ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac ggaggacaaa
    10501 caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc tcaaatgatc
    10561 acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat ttcgtttatc
    10621 caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca aatactggct
    10681 caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct aatacagctt
    10741 caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca aataaatacg
    10801 gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt atctacccag
    10861 gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt aatacaggtt
    10921 cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg tctcaaggca
    10981 cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac ggcatcacga
    11041 tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca ggacagcaat
    11101 tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact tctgcagcga
    11161 aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt aatacgatgt
    11221 ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat atcacgatgg
    11281 atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga cagcaagtga
    11341 ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt tcgaaaacct
    11401 atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga attacaatgg
    11461 ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt caaacgttga
    11521 ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca aaaatatttg
    11581 ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa aagactgaag
    11641 tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc gcctttgagt
    11701 tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa cgttcgaggc
    11761 cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa aaagagcaat
    11821 ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc aaaaggaaat
    11881 aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca agtgttttca
    11941 atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa aaatggcaaa
    12001 aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag ccaaaatttt
    12061 acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg catcctgaag
    12121 gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac aacgtgctgc
    12181 atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta gatacaggaa
    12241 acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa atcaattctt
    12301 taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag tattataaat
    12361 ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa gcagaagtag
    12421 cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg atcgatggga
    12481 aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg atgctgaaaa
    12541 tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg ccagaaagca
    12601 ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt gaatttaaag
    12661 tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact ctattcggtg
    12721 caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg acccctgaac
    12781 aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat ctaaagagaa
    12841 cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc aatccagtta
    12901 atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac ggcacaggag
    12961 ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa actttcggac
    13021 tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac acagaagatg
    13081 gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca atcgataaaa
    13141 tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat cgcttacgtg
    13201 actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc cattgggagg
    13261 acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct aagacaaaaa
    13321 acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag tgggtcaatg
    13381 tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg ccacaatggg
    13441 caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa gaactagcag
    13501 attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga gcagaacatg
    13561 cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat atcggtgttg
    13621 ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta ggcgaaaaca
    13681 acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg gtcgaaactt
    13741 acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat gcttcgattg
    13801 cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta tggcgtttga
    13861 tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat ggcaagcttg
    13921 ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat cttcatttca
    13981 atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg gacgctctgc
    14041 cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct cacatgggtg
    14101 aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca tggccaacat
    14161 acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta aacggtaaag
    14221 ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa aaagcagcat
    14281 tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa gttattgcgg
    14341 taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa taaggactga
    14401 tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca gattttttgt
    14461 tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt ctcaaacacg
    14521 ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat caaatttttg
    14581 tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt acataccaaa
    14641 ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca taatgaatga
    14701 agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag tattatcagc
    14761 taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt tcaagctttt
    14821 aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc tatatcgcca
    14881 tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt tgtatcactt
    14941 ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc tacttatttg
    15001 gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa gagcacaaca
    15061 gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg agaagacttc
    15121 gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga gatcacttga
    15181 tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca cattccatat
    15241 ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg gtggatacct
    15301 gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag ctggaacaat
    15361 ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg cttctcacat
    15421 gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt tgttagctga
    15481 tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac ttgatacaga
    15541 gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg tgccgattga
    15601 ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc ctgaaggtta
    15661 ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct attcttctgg
    15721 ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa cgtctattat
    15781 cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca acgatggtct
    15841 atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt acactactat
    15901 ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc cagtacaaaa
    15961 tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt tgttttatac
    16021 aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa actatcagac
    16081 aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg cgaatacgtt
    16141 agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa tgtttgcctg
    16201 cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac tatcgcaagt
    16261 agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg agtcacaaga
    16321 aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg attcggagtt
    16381 gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca agatcgtgtc
    16441 ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat tgtgacaagt
    16501 cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat cattagtatt
    16561 attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga tcatggacta
    16621 atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa attgactatt
    16681 ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc ctttttcgac
    16741 gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg acaaacggtg
    16801 tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct gaatcaatgc
    16861 ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag gaggattcgc
    16921 tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg tgccgttttc
    16981 gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat cacattgtgc
    17041 gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt aggcgatatt
    17101 atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa aagaacgacg
    17161 tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct ttcctttatt
    17221 cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac cagttggacc
    17281 aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca tcaatatttt
    17341 atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg gttgttctaa
    17401 aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct caaatatcct
    17461 atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg tagaaattgg
    17521 ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg tcacaccacc
    17581 tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt gattaatgaa
    17641 acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag cgttgtattg
    17701 ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga atgttttgac
    17761 cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca gttcatactg
    17821 attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa ttgaactgcc
    17881 aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca gcatcagacc
    17941 agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca ataacacttg
    18001 agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt gatgattgtc
    18061 aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca ccctcaatgt
    18121 gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga tgctgcttaa
    18181 gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga tatcactgaa
    18241 agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga tcccgattcc
    18301 catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata gaaacaatcc
    18361 gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag cacgcgtgtt
    18421 caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga ttttgcaaac
    18481 tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc aacgattttt
    18541 ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg ttgagcaaca
    18601 atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc tgcatctgtt
    18661 ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg tttgatcaac
    18721 gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc gccttgagca
    18781 acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg agcgatcttg
    18841 atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa gttgcttaaa
    18901 attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga gcggatctgt
    18961 tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat cgtagggtca
    19021 ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc gccaacgatc
    19081 gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg atcagagatt
    19141 tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc aaaggttggt
    19201 tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag tatttcttct
    19261 atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc tgcgatcatc
    19321 aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc gatttgtaat
    19381 gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac gattcccatg
    19441 agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat catttgatta
    19501 gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt atcaaaggca
    19561 cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg atccattttt
    19621 ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac aatccataca
    19681 taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg aaacatcatg
    19741 agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa taaaacaatc
    19801 atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc agaactaaaa
    19861 cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc ccgcgtttgg
    19921 aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc agcgattgtt
    19981 cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga ggcttcgcca
    20041 cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa tgtaccggcg
    20101 atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt ctgtaagaat
    20161 gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat tctatacctt
    20221 caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga gaaaaaatga
    20281 aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa tcaacgatcc
    20341 gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat gattatcgat
    20401 tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga aaattagata
    20461 taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta tatgagacac
    20521 ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa aaacaaaaag
    20581 agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag gaagaatttc
    20641 ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat attgtggaaa
    20701 tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca gcttccagtt
    20761 cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt actcaagacg
    20821 tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa tccgagatga
    20881 accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa aaaggatgga
    20941 aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac aatatcagaa
    21001 ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt taagataact
    21061 cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta cgggtttaat
    21121 gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa atatgtcttt
    21181 aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct cttaaaaaag
    21241 tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga gactaatgag
    21301 aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata aattaaatag
    21361 gtgcataaaa gcattacact taaaata
//
LOCUS       DARDHD010000031        20770 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000031 DARDHD010000000
VERSION     DARDHD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.031"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1139
                     /locus_tag="SDA06_000925"
     CDS             18..1139
                     /locus_tag="SDA06_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG4228220.1"
                     /translation="MVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPS
                     AGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGI
                     LIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAA
                     GISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALF
                     LGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLH
                     HRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIG
                     LINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1132..1932
                     /locus_tag="SDA06_000926"
     CDS             1132..1932
                     /locus_tag="SDA06_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4228221.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1932..2759
                     /locus_tag="SDA06_000927"
     CDS             1932..2759
                     /locus_tag="SDA06_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4228222.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2737..3471
                     /locus_tag="SDA06_000928"
     CDS             2737..3471
                     /locus_tag="SDA06_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4228223.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3571..4437
                     /gene="rfbA"
                     /locus_tag="SDA06_000929"
     CDS             3571..4437
                     /gene="rfbA"
                     /locus_tag="SDA06_000929"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG4228224.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4451..5023
                     /gene="rfbC"
                     /locus_tag="SDA06_000930"
     CDS             4451..5023
                     /gene="rfbC"
                     /locus_tag="SDA06_000930"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG4228225.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5045..6073
                     /gene="rfbB"
                     /locus_tag="SDA06_000931"
     CDS             5045..6073
                     /gene="rfbB"
                     /locus_tag="SDA06_000931"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG4228226.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6171..7022
                     /gene="rfbD"
                     /locus_tag="SDA06_000932"
     CDS             6171..7022
                     /gene="rfbD"
                     /locus_tag="SDA06_000932"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG4228227.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7057..9090
                     /locus_tag="SDA06_000933"
     CDS             7057..9090
                     /locus_tag="SDA06_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228228.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9134..10414
                     /locus_tag="SDA06_000934"
     CDS             9134..10414
                     /locus_tag="SDA06_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG4228229.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10624..11430
                     /locus_tag="SDA06_000935"
     CDS             10624..11430
                     /locus_tag="SDA06_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228230.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11442..12662
                     /locus_tag="SDA06_000936"
     CDS             11442..12662
                     /locus_tag="SDA06_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228231.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12652..14238
                     /locus_tag="SDA06_000937"
     CDS             12652..14238
                     /locus_tag="SDA06_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4228232.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14277..16415
                     /locus_tag="SDA06_000938"
     CDS             14277..16415
                     /locus_tag="SDA06_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4228233.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16783..17766)
                     /locus_tag="SDA06_000939"
     CDS             complement(16783..17766)
                     /locus_tag="SDA06_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG4228234.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18144..19535
                     /locus_tag="SDA06_000940"
     CDS             18144..19535
                     /locus_tag="SDA06_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4228235.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19551..20591
                     /locus_tag="SDA06_000941"
     CDS             19551..20591
                     /locus_tag="SDA06_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4228236.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20610..>20770
                     /locus_tag="SDA06_000942"
     CDS             20610..>20770
                     /locus_tag="SDA06_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4228237.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIE"
BASE COUNT         6647 a         3445 c         4279 g         6399 t
ORIGIN      
        1 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
       61 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
      121 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
      181 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
      241 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
      301 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
      361 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
      421 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
      481 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
      541 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
      601 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
      661 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
      721 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
      781 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
      841 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
      901 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
      961 cgatcgtatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     1021 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     1081 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     1141 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     1201 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     1261 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     1321 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     1381 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     1441 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     1501 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     1561 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     1621 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     1681 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     1741 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     1801 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     1861 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     1921 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     1981 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     2041 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     2101 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     2161 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     2221 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     2281 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     2341 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     2401 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     2461 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     2521 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     2581 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     2641 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     2701 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     2761 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     2821 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     2881 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     2941 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     3001 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     3061 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     3121 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     3181 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     3241 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     3301 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     3361 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     3421 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     3481 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     3541 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     3601 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     3661 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     3721 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     3781 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     3841 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     3901 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     3961 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     4021 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     4081 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     4141 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     4201 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     4261 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     4321 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     4381 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     4441 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     4501 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     4561 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
     4621 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
     4681 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
     4741 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
     4801 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
     4861 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
     4921 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
     4981 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
     5041 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
     5101 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
     5161 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
     5221 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
     5281 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
     5341 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
     5401 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
     5461 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
     5521 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
     5581 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
     5641 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
     5701 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
     5761 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
     5821 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
     5881 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
     5941 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
     6001 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
     6061 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
     6121 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
     6181 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
     6241 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
     6301 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
     6361 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
     6421 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
     6481 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
     6541 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
     6601 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
     6661 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
     6721 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
     6781 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
     6841 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
     6901 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
     6961 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
     7021 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
     7081 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
     7141 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
     7201 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
     7261 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
     7321 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
     7381 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
     7441 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
     7501 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
     7561 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
     7621 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
     7681 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
     7741 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
     7801 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
     7861 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
     7921 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
     7981 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
     8041 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
     8101 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
     8161 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
     8221 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
     8281 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
     8341 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
     8401 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
     8461 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
     8521 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
     8581 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
     8641 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
     8701 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
     8761 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
     8821 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
     8881 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
     8941 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
     9001 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
     9061 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
     9121 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
     9181 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
     9241 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
     9301 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
     9361 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
     9421 tggagactta ctgtttcctt tggttatttt attttgttta gtaattaaat ggatcgagtg
     9481 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
     9541 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
     9601 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
     9661 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
     9721 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
     9781 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
     9841 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
     9901 ggctgtctct gaaaaagtca atttgtttac cgatcgagaa gatatttggc ataaattcta
     9961 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    10021 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    10081 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    10141 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    10201 gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta tttatatcct
    10261 gttggctatg gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat
    10321 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    10381 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    10441 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
    10501 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
    10561 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
    10621 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg
    10681 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
    10741 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
    10801 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
    10861 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
    10921 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
    10981 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
    11041 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
    11101 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
    11161 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
    11221 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
    11281 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
    11341 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
    11401 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
    11461 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
    11521 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
    11581 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
    11641 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
    11701 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
    11761 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
    11821 tggtcgatga tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa
    11881 cttattcaag cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg
    11941 atattttgat tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta
    12001 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
    12061 tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg
    12121 aatatggcga tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag
    12181 ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg
    12241 acgtgacgaa agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag
    12301 gagaaaaact atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg
    12361 ttttaggaat tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac
    12421 tagatggtat caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa
    12481 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
    12541 cggttccttt ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg
    12601 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
    12661 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
    12721 ccaggaagta aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc
    12781 ggagcaaaac aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc
    12841 agcgaatttg ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat
    12901 tatgctggac agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat
    12961 cctggaaaaa cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt
    13021 acattcccta acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg
    13081 tgggcttctt atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc
    13141 caaaaagttt ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct
    13201 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat
    13261 ttcaaaatgc gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca
    13321 gtagcacaaa gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta
    13381 acccagcaaa cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg
    13441 gaaaatgaga cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc
    13501 gcgcaacaac ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt
    13561 atcgattcga atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa
    13621 ttaccagagt tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat
    13681 cgctttatcg gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg
    13741 gctgaagtca caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat
    13801 cttcttgagg aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc
    13861 acattgttgg aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa
    13921 acttcgaaaa aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat
    13981 gcagaaacaa aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc
    14041 ttgtcctata agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga
    14101 gaagaagtca atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga
    14161 atcctttgcg agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca
    14221 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
    14281 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
    14341 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
    14401 aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat
    14461 ataatcttga tgttaagacc caaagcggtt tcaaggtacg tttatcaggg aaaatgagag
    14521 gaaaagccat cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa
    14581 acggtaaata cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
    14641 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca
    14701 aattggaatt gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
    14761 cagatttgaa agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca
    14821 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
    14881 tggatcaaac gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag
    14941 ctatccgaaa gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc
    15001 gaaaagaaaa tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag
    15061 aatttatagc actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg
    15121 caaaagtgct aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg
    15181 atgcagatgg caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga
    15241 tgggaaataa ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg
    15301 gcggtttccg taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag
    15361 aacaaatccc tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga
    15421 tcccaggatc aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga
    15481 aagcattgac agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa
    15541 tttctggatt ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca
    15601 ttccaacgaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
    15661 cgtcttattc aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc
    15721 aagaattatt tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg
    15781 acattccatt caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat
    15841 actttttatt cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg
    15901 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg
    15961 atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga
    16021 ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact
    16081 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt
    16141 ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
    16201 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
    16261 gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc
    16321 aagataaatg gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag
    16381 acggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg
    16441 tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc
    16501 gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa
    16561 attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct
    16621 tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc
    16681 ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg
    16741 aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg
    16801 tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt
    16861 gatggagata accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat
    16921 tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt
    16981 ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg
    17041 tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag
    17101 atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa
    17161 ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc
    17221 agcataataa ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa
    17281 atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac
    17341 agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt
    17401 ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg
    17461 tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt
    17521 cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg
    17581 ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca
    17641 agtatcgtct tttttataac cattccattc atctgtataa acttctaccc catccttata
    17701 tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt
    17761 attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca
    17821 attttttcga ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag
    17881 atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata
    17941 cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt
    18001 tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca
    18061 ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt
    18121 attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga
    18181 tattcattat attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac
    18241 tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta
    18301 ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc
    18361 gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga
    18421 tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt
    18481 catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt
    18541 tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag
    18601 caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg
    18661 ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt
    18721 ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa
    18781 aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt
    18841 tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat
    18901 tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta
    18961 tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag
    19021 tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca
    19081 cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg
    19141 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
    19201 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc tttcttcgtc gatcagttca
    19261 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
    19321 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
    19381 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
    19441 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
    19501 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
    19561 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg
    19621 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
    19681 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
    19741 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
    19801 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
    19861 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
    19921 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
    19981 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
    20041 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
    20101 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
    20161 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
    20221 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
    20281 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
    20341 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
    20401 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
    20461 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
    20521 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
    20581 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
    20641 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
    20701 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
    20761 agattgaaag
//
LOCUS       DARDHD010000032        20680 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000032 DARDHD010000000
VERSION     DARDHD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.032"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            281..1360
                     /locus_tag="SDA06_000943"
     CDS             281..1360
                     /locus_tag="SDA06_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4228238.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1557..2258)
                     /locus_tag="SDA06_000944"
                     /pseudo
     CDS             complement(1557..2258)
                     /locus_tag="SDA06_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2272..3196)
                     /locus_tag="SDA06_000945"
                     /pseudo
     CDS             complement(2272..3196)
                     /locus_tag="SDA06_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3313..4392
                     /locus_tag="SDA06_000946"
     CDS             3313..4392
                     /locus_tag="SDA06_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4228239.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4424..5017
                     /locus_tag="SDA06_000947"
     CDS             4424..5017
                     /locus_tag="SDA06_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228240.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(5127..5354)
                     /locus_tag="SDA06_000948"
     CDS             complement(5127..5354)
                     /locus_tag="SDA06_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4228241.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5457..8045)
                     /locus_tag="SDA06_000949"
     CDS             complement(5457..8045)
                     /locus_tag="SDA06_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG4228242.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(8065..8724)
                     /locus_tag="SDA06_000950"
     CDS             complement(8065..8724)
                     /locus_tag="SDA06_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4228243.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8773..9276)
                     /gene="trmL"
                     /locus_tag="SDA06_000951"
     CDS             complement(8773..9276)
                     /gene="trmL"
                     /locus_tag="SDA06_000951"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG4228244.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9497..10363)
                     /locus_tag="SDA06_000952"
     CDS             complement(9497..10363)
                     /locus_tag="SDA06_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4228245.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10472..11113
                     /locus_tag="SDA06_000953"
     CDS             10472..11113
                     /locus_tag="SDA06_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG4228246.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(11175..12536)
                     /gene="mgtE"
                     /locus_tag="SDA06_000954"
     CDS             complement(11175..12536)
                     /gene="mgtE"
                     /locus_tag="SDA06_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG4228247.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12554..13453)
                     /locus_tag="SDA06_000955"
     CDS             complement(12554..13453)
                     /locus_tag="SDA06_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4228248.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13455..14252)
                     /locus_tag="SDA06_000956"
     CDS             complement(13455..14252)
                     /locus_tag="SDA06_000956"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG4228249.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(14230..14934)
                     /locus_tag="SDA06_000957"
     CDS             complement(14230..14934)
                     /locus_tag="SDA06_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG4228250.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            15065..15640
                     /locus_tag="SDA06_000958"
     CDS             15065..15640
                     /locus_tag="SDA06_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG4228251.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15758..16435
                     /locus_tag="SDA06_000959"
     CDS             15758..16435
                     /locus_tag="SDA06_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4228252.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16514..18322)
                     /gene="pepF"
                     /locus_tag="SDA06_000960"
     CDS             complement(16514..18322)
                     /gene="pepF"
                     /locus_tag="SDA06_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG4228253.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18498..19496)
                     /locus_tag="SDA06_000961"
     CDS             complement(18498..19496)
                     /locus_tag="SDA06_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG4228254.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19722..20375)
                     /locus_tag="SDA06_000962"
     CDS             complement(19722..20375)
                     /locus_tag="SDA06_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG4228255.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5989 a         4486 c         3684 g         6521 t
ORIGIN      
        1 aaaagttcac tactctctgc tgttacatcg gaattactca tttcagtcgt ctcggttctt
       61 tcttttgcaa ttttgtatgt tgaattttcg cttgccaaca catggaaagg tgttgctata
      121 aggagttggg acattaatat aactgctgat gcaagtttta aagatttttt cattggcgga
      181 ttttataatg aacttagcca tttaaaataa aaaaaacgcc acgtgaattc catgttatat
      241 tgaagttacc acaactctca atagacaagg atgaattcac atgacgcaaa cccatgataa
      301 cacagtttca cgcaagggaa aacatctttc ctactcagaa cgttgccaaa tcgctatcct
      361 gaaaaaggaa ggctattcca accgtcaggt tgccaaagtg ctgggacgtg ttccgcaaac
      421 cataaacaat gagattgatc gcggaaccat cacccagtta aaacgccaaa cgcaaaatgg
      481 aaaagtgtat gacacctact attctgttta tgacgctgat gcaggacagg ccttctatga
      541 aaagcaacga ctgaactgtg gcagacgccc caaatgggcg aacacaaatg ccttcattga
      601 ttgggcagat gacaaaatgt taaatgaaaa atggtcgccg gacgtcgtgg tcggatttgc
      661 cagaaaacac gagctgttcg attgtgccat cattcctggc acaaccgctc tttatcaatg
      721 gattgaccgc ggaatcatgc gaacaaagaa catggacttg cttgaaaaac tgtcccgtaa
      781 aatcaaagcg gtccgcccag gacaccgtcc caacaagcgt gttctcggga cgtcaattga
      841 agaacgccct aaagaagttg aaacacggga aacgttcggt cattgggaaa tcgacacggt
      901 agtgggaaac aagaccaagt cggatgccgt gctgctgacg ctggctgaac ggcagacacg
      961 ctttgaagtc atcctaaagt tgaacgggaa ggacaccgaa tcggtggacc aagcggttca
     1021 acagctaaga gaacgggctg gcgctaactt cgatcaatta tttaagaccg ttacatccga
     1081 caatggctcg gagttttctg ggctacacga tgctctgcaa gatgtagctg acgtctattt
     1141 cagccacccc tatgcttctt gggagcgtgg gacgagcgaa aaccagcaca aattaattcg
     1201 ccgtttcgtc cccaagggaa gcccgattag tgaggtgagc gagaaccaga ttttgcggat
     1261 ccagcgttgg atgaacgatt acccgcggaa aattttgggt tatgcgacac cacatgatac
     1321 attcgtccag gcattcaagc aagaacggtt ggtggcttga cccattattc cttgataacg
     1381 atcctcactc gggacatggc ttcatgtctg gaaagctggc ctggggccag aatcgtctga
     1441 agactatcat acctcagttc attatcaagg cctcactgat ttagaataac gaggcagtta
     1501 cggtatcaaa caaatgagtt acctgtagtg gctaacttaa acttgaaatt tacggacgta
     1561 aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat caaggacaag
     1621 cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac cccccacatc
     1681 gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt gaaactcata
     1741 ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt catgatgggc
     1801 gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat acgctgtaaa
     1861 aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc cacatggagc
     1921 gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt tagcttttca
     1981 ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt gacttcgact
     2041 tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt gattacaatt
     2101 ccagtattaa tcgtttcgag attgccacct tgatcaatct cgccaaaggc cgccataaac
     2161 atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt atttcgcagc
     2221 agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc tctagtctct
     2281 caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt tggttgctat
     2341 ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca atactgtatt
     2401 tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat cagaagctat
     2461 tgttcgctga caacacgttt tctgggagta atgggatgtc gatcgtcaaa cgatccgccg
     2521 tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt ccttgatcca
     2581 tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt gtatagacaa
     2641 tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc cgactttggg
     2701 ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac
     2761 atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga aacgaaacat
     2821 atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt ttctcttttt
     2881 cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt tcgtcaaaga
     2941 ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt tcaggagtca
     3001 ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa agcatacagt
     3061 tgatggcagt cgatttccca ctgccgtttg gtcctaacag acctaagact tctccttctt
     3121 gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg actaatttat
     3181 caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct gactataata
     3241 aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt tgtcacaatg
     3301 aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct actggttatc
     3361 tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta tttattgatc
     3421 gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg gattttccaa
     3481 gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct gccagcgacg
     3541 atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt aggttttttg
     3601 ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt aattttgctg
     3661 tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga gaaaaagtac
     3721 attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca aaataaagca
     3781 ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg gaaccgaatt
     3841 gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt
     3901 gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc
     3961 accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca tgagacatcg
     4021 atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc tgttacgatt
     4081 gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt aatggtcatc
     4141 aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt tttacatttt
     4201 cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa
     4261 aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga
     4321 caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt accaagggag
     4381 ggacaccaat gataacagta gtcattattg agatgatcca tttgtgacga aatcgttaca
     4441 aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt gtgccaagga
     4501 tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc
     4561 taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca aagccatcct
     4621 tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt ccatcggtgt
     4681 caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca aatccgctta
     4741 taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa tgatgaagaa
     4801 taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat
     4861 caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt acttgagtga
     4921 cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac gggatcgcac
     4981 gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca caaaaaaacc
     5041 tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca attgtcccgc
     5101 aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg attcgttgga
     5161 ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc tagttttgat
     5221 tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc tttttccatc
     5281 tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa tttatcttcg
     5341 gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata tcacactgaa
     5401 aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct ttaaaatcaa
     5461 gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat catcggatct
     5521 actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac agcaggttct
     5581 acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc cactgtttcc
     5641 attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag cttttctttt
     5701 acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc gaccaaagaa
     5761 tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt atccatgatc
     5821 cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt gacataggct
     5881 tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt gtagagcaaa
     5941 ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat cttgaattca
     6001 ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt ccattcgttt
     6061 cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac tttatcttca
     6121 gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa tacattcaat
     6181 tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt gtcgttccaa
     6241 tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat tttatttaac
     6301 gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc ttgtgcacgg
     6361 aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat ttgataagca
     6421 tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg cagacgtcct
     6481 tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg ataaatctga
     6541 ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac ttgaccagga
     6601 ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt cgtcggaatc
     6661 gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc aacaatcaaa
     6721 agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc attcagcccc
     6781 agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg tttagctgct
     6841 cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt tgaaggatcc
     6901 aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac agtagttttc
     6961 cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc agcgatagct
     7021 tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg aatattcttc
     7081 tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg ctggatcgaa
     7141 ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt ctcttcttgt
     7201 tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc aggctggatt
     7261 tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt ctccgccggt
     7321 acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat agctgctcga
     7381 attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc tgcacgtttg
     7441 aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat gatatccaaa
     7501 gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa cccgtaacga
     7561 ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc agcaagcatt
     7621 tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc agcaatgatt
     7681 ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt cttcccgatt
     7741 cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt tggcttttct
     7801 tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac taattctcca
     7861 taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac tacaatctct
     7921 ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt gtaaaaatta
     7981 ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat ttcttctggc
     8041 tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt cagttcctaa
     8101 aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag gctcttcagt
     8161 ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact cacttagctc
     8221 ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta cgaataaaag
     8281 tgggccatct ttatgttggg caaccgcatc tgctacaaca gtttcgatac gtgtgattgc
     8341 cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat gattacgcaa
     8401 attcggcaga ttatccggat atttctggta catttcatcg atcgactgac cttctaaagt
     8461 acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac gagcaagctc
     8521 tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat aaattgcttt
     8581 aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac tttgtggcaa
     8641 aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt gtgtcttccc
     8701 atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt attttactct
     8761 ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt ctaaattagg
     8821 gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg ataaattcaa
     8881 tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt tttcacgcat
     8941 aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct cttctccatc
     9001 agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat gtagtttacg
     9061 attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat ttacatcatt
     9121 ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac ctagtggctc
     9181 gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc cagtgtttgc
     9241 agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc tcatatttat
     9301 gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat caactcggtg
     9361 tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta tcagaacagg
     9421 aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct caactttttt
     9481 ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt ccttttaata
     9541 attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct ttgattcgct
     9601 cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt tcctctggta
     9661 tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca gcaaacttgg
     9721 ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa aaagcctgac
     9781 gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca tcttctgaga
     9841 gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc gcgttgaatg
     9901 tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct tcggcagatt
     9961 gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca ttttgaatct
    10021 ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca tctaatagcg
    10081 agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac atgccagatt
    10141 ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta accgcatggg
    10201 ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt ttgcatatca
    10261 agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac agctgatgat
    10321 tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc cctcatttct
    10381 gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat acaatgccta
    10441 gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg ctgaaaacta
    10501 cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt gtgacaactt
    10561 agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa gttatgctgg
    10621 ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact tcgtctataa
    10681 cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat tagggacgga
    10741 tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg ccttagaact
    10801 attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg acagtattcc
    10861 tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg accgtatctt
    10921 gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc ttaaagaatt
    10981 gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca ctgcagagaa
    11041 tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca aaatcgtgga
    11101 tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg cttattgcaa
    11161 atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt gaaataaatc
    11221 agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac tgctggatca
    11281 aatcctagac gatccattag catcgggatt aaactacctg caagattcgc cactgtgatt
    11341 gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt ccaaaaacct
    11401 acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt caagacttca
    11461 gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag tcgacgaaca
    11521 gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag cgaaatgaag
    11581 actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa agaagcggtc
    11641 gacataccca aaaacaacag agtgatcagc caaggcaaac gtcgagacgc tgcctttact
    11701 ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc gctagcagct
    11761 tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa atgatcatca
    11821 taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc cacatcttct
    11881 tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga aatcattgcg
    11941 tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg accttcttga
    12001 ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt cagcacgtac
    12061 atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt catgatcgcg
    12121 ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc gtctgccgac
    12181 atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa gtcaacagca
    12241 ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat ttcatctagg
    12301 tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc taattcttta
    12361 ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg gtagaattgc
    12421 ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat attattttcc
    12481 cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc atccaaacca
    12541 atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct gcggctaaag
    12601 gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga tcataacaat
    12661 gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca tcaccgagca
    12721 gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct gtatgcagct
    12781 ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca gtctgtgccg
    12841 aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg gcgatcggca
    12901 aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag gcctgatatt
    12961 ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca aatccatgct
    13021 ttgcaaaaag catcaaacca gatgtatccc gatctagtcg cgtcaccaca tggattactt
    13081 ggttttcgta accttgtttt ttataataag cctttacacg attagccatt gtcccatttg
    13141 gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc agaacgtgtt
    13201 catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca tgttctcctt
    13261 cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag acattttcta
    13321 cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac agaccctttg
    13381 agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc tcataatccc
    13441 atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg ccaaaaatgc
    13501 atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata aattgagcgg
    13561 atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc tgtattttcc
    13621 aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa agtacgaaac
    13681 actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact aggatggatc
    13741 acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga aatcgtcaat
    13801 ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc taccatcgta
    13861 cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc cggtgtttca
    13921 tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc ccggttcgtt
    13981 cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta aaaccctaaa
    14041 tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta gtgatggaac
    14101 gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc atgatcatct
    14161 cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc tatagtcttt
    14221 gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt cgctttcttt
    14281 cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg agaataaccg
    14341 ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact gataggcagc
    14401 ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt gatatttata
    14461 attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa tctggatttc
    14521 cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca ccagatgata
    14581 agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga gaactttcat
    14641 gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa actgacacat
    14701 gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg gaatatgccg
    14761 taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga caaattcaat
    14821 cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt tcactttcaa
    14881 ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt ccatagctga
    14941 tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt tttttcgtac
    15001 tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa aaggagtcga
    15061 tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc aagagtacga
    15121 gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga ctaatattta
    15181 tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga tccgtttcgt
    15241 tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc tcgaaacaac
    15301 ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc tgcctcgttc
    15361 aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat taatccaaac
    15421 cggaaagctt acaacaaaac gggctgaatt ccctattgaa gaaggacttt tagctattga
    15481 tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag gggatgcatc
    15541 tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc
    15601 taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag
    15661 gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt
    15721 cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt
    15781 taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga
    15841 agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt
    15901 gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg
    15961 taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca
    16021 aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga
    16081 cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa
    16141 tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa
    16201 aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg
    16261 cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac
    16321 agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac
    16381 aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta attaaaaaaa
    16441 tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca
    16501 aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa
    16561 acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca
    16621 ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg
    16681 cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg
    16741 agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa
    16801 tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta ccataactat
    16861 cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct
    16921 cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt
    16981 tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga
    17041 tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat
    17101 aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg
    17161 taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc
    17221 ttcccgaaga ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt
    17281 cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta
    17341 aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg
    17401 tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt
    17461 atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt
    17521 caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg
    17581 ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt
    17641 gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt
    17701 ctagcaactg accatacacg ccatgagata attggactat ttcaccattt tctccttcaa
    17761 tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa
    17821 agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat
    17881 tatctactat ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat
    17941 attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt
    18001 cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc
    18061 cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa
    18121 ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt
    18181 tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt
    18241 ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt
    18301 ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca
    18361 aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt
    18421 gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta
    18481 gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac
    18541 tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt
    18601 atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac
    18661 aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag
    18721 atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc ttgtatggat
    18781 actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc atcttcactc
    18841 actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa tatttcttta
    18901 aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc
    18961 tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt ccagcaatgc
    19021 actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt
    19081 ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa ataattttgg
    19141 gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat tgctagattc
    19201 gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa aaatactggt
    19261 tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg ctcttttgat
    19321 tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg agcacatttt
    19381 ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata tttctttcct
    19441 ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag cagcattttt
    19501 ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa
    19561 agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa tatttttcgt
    19621 ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga aaaaaagact
    19681 gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa ataaaatttc
    19741 gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa tgtttctggt
    19801 gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc tacatccgct
    19861 tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc gaataaatac
    19921 aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa
    19981 tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc agataattct
    20041 tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc ttctgaatca
    20101 acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact gataaacagt
    20161 tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc tcggaactcc
    20221 tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc tttgtgattg
    20281 ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc aattagtaca
    20341 cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac tttgccacaa
    20401 tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct
    20461 gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc ttatttaact
    20521 ataacatttt tggcagacga atactatata gccgactttt cagctaattt tccctctcta
    20581 tttttcgtgc aatcatcatc ttctactacc tttttataca tctaatcaag aatacttttt
    20641 gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag
//
LOCUS       DARDHD010000033        20634 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000033 DARDHD010000000
VERSION     DARDHD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.033"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..395
                     /locus_tag="SDA06_000963"
     CDS             27..395
                     /locus_tag="SDA06_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG4228256.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            565..996
                     /gene="mraZ"
                     /locus_tag="SDA06_000964"
     CDS             565..996
                     /gene="mraZ"
                     /locus_tag="SDA06_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG4228257.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1017..1976
                     /gene="rsmH"
                     /locus_tag="SDA06_000965"
     CDS             1017..1976
                     /gene="rsmH"
                     /locus_tag="SDA06_000965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG4228258.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1981..2382
                     /gene="ftsL"
                     /locus_tag="SDA06_000966"
     CDS             1981..2382
                     /gene="ftsL"
                     /locus_tag="SDA06_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG4228259.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2383..4575
                     /locus_tag="SDA06_000967"
     CDS             2383..4575
                     /locus_tag="SDA06_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4228260.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4601..5563
                     /locus_tag="SDA06_000968"
     CDS             4601..5563
                     /locus_tag="SDA06_000968"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG4228261.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5577..6938
                     /locus_tag="SDA06_000969"
     CDS             5577..6938
                     /locus_tag="SDA06_000969"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG4228262.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6964..8052
                     /gene="murG"
                     /locus_tag="SDA06_000970"
     CDS             6964..8052
                     /gene="murG"
                     /locus_tag="SDA06_000970"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG4228263.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8067..9287
                     /locus_tag="SDA06_000971"
     CDS             8067..9287
                     /locus_tag="SDA06_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG4228264.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9443..10768
                     /gene="ftsA"
                     /locus_tag="SDA06_000972"
     CDS             9443..10768
                     /gene="ftsA"
                     /locus_tag="SDA06_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG4228265.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10791..12032
                     /gene="ftsZ"
                     /locus_tag="SDA06_000973"
     CDS             10791..12032
                     /gene="ftsZ"
                     /locus_tag="SDA06_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG4228266.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12049..12726
                     /locus_tag="SDA06_000974"
     CDS             12049..12726
                     /locus_tag="SDA06_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG4228267.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12740..13339
                     /locus_tag="SDA06_000975"
     CDS             12740..13339
                     /locus_tag="SDA06_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG4228268.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13354..13641
                     /locus_tag="SDA06_000976"
     CDS             13354..13641
                     /locus_tag="SDA06_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG4228269.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13690..14472
                     /locus_tag="SDA06_000977"
     CDS             13690..14472
                     /locus_tag="SDA06_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG4228270.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14493..15200
                     /locus_tag="SDA06_000978"
     CDS             14493..15200
                     /locus_tag="SDA06_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG4228271.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15462..18260
                     /gene="ileS"
                     /locus_tag="SDA06_000979"
     CDS             15462..18260
                     /gene="ileS"
                     /locus_tag="SDA06_000979"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG4228272.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18309..19835)
                     /locus_tag="SDA06_000980"
     CDS             complement(18309..19835)
                     /locus_tag="SDA06_000980"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG4228273.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19850..20497)
                     /locus_tag="SDA06_000981"
     CDS             complement(19850..20497)
                     /locus_tag="SDA06_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG4228274.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6978 a         3570 c         4414 g         5672 t
ORIGIN      
        1 caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc ttgttttggt
       61 atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa
      121 atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc
      181 atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt atttctgttt
      241 tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa attatttatc
      301 cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg
      361 tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt
      421 cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata aaaaaagttg
      481 ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac taattttagt
      541 tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa tattgatgca
      601 aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg
      661 acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga aaatctggaa
      721 gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt gcgattcttc
      781 tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat acccagcacg
      841 ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt
      901 gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga gaactttgac
      961 gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc tgactaatga
     1021 ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga ttgaacatca
     1081 aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt gaatatttat
     1141 taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg
     1201 atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca tttatcaaag
     1261 cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta gacggcattt
     1321 tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga tttagttatc
     1381 atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc gcttatcacg
     1441 taattaatga atattcctac catgaattag taaagatttt cttccgctat ggcgaggaga
     1501 aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa
     1561 caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca agaagaaaag
     1621 gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat gacgaactcg
     1681 gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat ggaagaatca
     1741 gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt aaagaatata
     1801 gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc caaccagaac
     1861 tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca gaaaacaatc
     1921 gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag gagtagaatg
     1981 atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt ggtggatcag
     2041 ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg
     2101 gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt
     2161 gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag cggagctgaa
     2221 aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa aacgagttta
     2281 gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc agaagaaaaa
     2341 ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct taaaaataaa
     2401 attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt
     2461 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
     2521 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
     2581 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
     2641 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
     2701 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
     2761 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
     2821 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
     2881 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
     2941 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
     3001 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
     3061 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
     3121 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
     3181 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
     3241 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
     3301 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
     3361 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
     3421 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
     3481 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
     3541 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
     3601 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
     3661 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
     3721 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
     3781 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
     3841 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
     3901 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat
     3961 agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc ttctgataca
     4021 gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat ggttccttct
     4081 gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata tgatcgaatg
     4141 gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa agaaactgaa
     4201 gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta tcgtaatctt
     4261 gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt
     4321 aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat ctctggagaa
     4381 aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg atggtcaaaa
     4441 gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga tggagatggt
     4501 tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa acttagtttt
     4561 actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga cacaagcatt
     4621 gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat ttattggtta
     4681 ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag
     4741 caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta ttttgacagg
     4801 aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct tgctttttgt
     4861 attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat tcaagaaaag
     4921 aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg gaatcatctt
     4981 ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg gactagatct
     5041 acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat tttcaaatgc
     5101 agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta tctcttttgc
     5161 agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca tttgcttaag
     5221 tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca agatttttat
     5281 gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta taatgctaaa
     5341 ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag caagtgtgat
     5401 gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt ctccaattca
     5461 tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt tttggttgat
     5521 cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga cgggaaatga
     5581 aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca aaaagcggag
     5641 taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat gatggaaaac
     5701 cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa gtaataacag
     5761 gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa aatccaggga
     5821 ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg gtaattacgg
     5881 aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca ggaacgaacg
     5941 gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca gatcaaggaa
     6001 ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat
     6061 cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc acagatttcc
     6121 gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat catggtacaa
     6181 gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa aaagattatc
     6241 tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca aaagcacgcg
     6301 tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac gattactcaa
     6361 tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca ggtaagcata
     6421 atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata tcaaatgaag
     6481 ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag
     6541 aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta gcgactaaaa
     6601 tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga ttagacagag
     6661 gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg gtcgtatttg
     6721 gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag acaatagtaa
     6781 cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca gatggtgatg
     6841 tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt gaaactagag
     6901 gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag cgagcgataa
     6961 aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc agctcttgcc
     7021 tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa
     7081 cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac cttggagatt
     7141 caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt gtttcttaag
     7201 agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt tatcggtact
     7261 ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat tccaacagtt
     7321 atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg atatgtggat
     7381 cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa agcagttctt
     7441 gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat tttgaaaaca
     7501 tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca aggagcactt
     7561 aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc ttaccaagtt
     7621 ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa tcctgaaata
     7681 caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc ttccagtgat
     7741 ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct ggggttaccg
     7801 gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa tgcaatgagt
     7861 cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg tgaatcacta
     7921 agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat gtgccgagct
     7981 tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt aaaacaaata
     8041 atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc
     8101 atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga aaatatctgg
     8161 cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta gatacacccc
     8221 tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa agccagtccg
     8281 agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga ccatataacg
     8341 gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc
     8401 ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc tattatgttt
     8461 ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc aacagccaag
     8521 aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag tattttcatc
     8581 gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca gcaaatatac
     8641 atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag attgctttga
     8701 tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta gatgaaaaag
     8761 tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct gcaaaaatca
     8821 aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca atttctgaaa
     8881 tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg aatgacggca
     8941 atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat tcgcaagtag
     9001 ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt
     9061 acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact gaaagtgaag
     9121 aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac
     9181 ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa
     9241 tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa aacaacttta
     9301 aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga agcagtgaat
     9361 atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta tatggaatag
     9421 taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg
     9481 aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga atattattgg
     9541 agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata tcgataaaac
     9601 ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta tccagatcaa
     9661 aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc agggaatgat
     9721 tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg tggcttcagc
     9781 tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt
     9841 tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg taagattaga
     9901 aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac gtaaatgcgt
     9961 ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc tgactgaatc
    10021 aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg gtggcggcca
    10081 aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg accaagaagg
    10141 tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta acaatgcaga
    10201 agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag atgaagaatt
    10261 tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct acttgtcaga
    10321 aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat tggaccaaat
    10381 cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg
    10441 tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca
    10501 catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt attcagcaaa
    10561 cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg
    10621 tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag cacctgaaga
    10681 aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa
    10741 gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat atggaattct
    10801 caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc ggtggtggcg
    10861 gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa ttcattacag
    10921 ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc caattaggcc
    10981 ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa aaagcggcag
    11041 aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt ttcatcactg
    11101 caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag
    11161 aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac
    11221 gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat acattattaa
    11281 ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg cttgaagctt
    11341 tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat ttgatcacag
    11401 cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa aaccaaggta
    11461 cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa
    11521 aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa gtacttctta
    11581 acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca gatatcgtag
    11641 caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat gaagaaatgg
    11701 gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca
    11761 agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag actgtattag
    11821 atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc gattgggata
    11881 tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac aatatcgaaa
    11941 aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa
    12001 atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat gattgctgac
    12061 aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca tcgtgatccg
    12121 aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg
    12181 gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa
    12241 caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt acagcgcaga
    12301 aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag tttaagatta
    12361 gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga agtaaatgtt
    12421 tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt tgtggaatct
    12481 ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc taaagaagct
    12541 tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga tcttgttcaa
    12601 gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag tcaagacttt
    12661 caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct attcaaagac
    12721 gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta attttttcgg
    12781 attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa aaaaggctgt
    12841 acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat ctgctgcgaa
    12901 tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa gtattcagcc
    12961 ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg tagcgaaaga
    13021 aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa aaatcactgt
    13081 aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca ttgctggaga
    13141 agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa tcgttgattt
    13201 tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag atgagatttt
    13261 tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt ttgcagatgc
    13321 gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact attacttcat
    13381 ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc attgctctct
    13441 tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat ctgcgaaccg
    13501 tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt cacaatttta
    13561 ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt tatatggata
    13621 actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa tagataggag
    13681 gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
    13741 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
    13801 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
    13861 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
    13921 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
    13981 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    14041 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    14101 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    14161 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    14221 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    14281 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    14341 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    14401 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    14461 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    14521 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    14581 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    14641 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    14701 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    14761 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    14821 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    14881 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    14941 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    15001 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    15061 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    15121 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    15181 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    15241 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    15301 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    15361 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    15421 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    15481 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    15541 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    15601 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    15661 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    15721 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    15781 ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat
    15841 atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga gttgcaggag
    15901 attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa gtacgcgtat
    15961 ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt tactggtctc
    16021 cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta aaatctccat
    16081 ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa gatacagctt
    16141 ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc
    16201 caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt
    16261 tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta aaagaatttg
    16321 ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt gaatcacttg
    16381 tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat actgctccag
    16441 gacatgggga agatgactat atcgctggta gcaaatataa actatctgta gtgtcacctg
    16501 tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc ttttatgaca
    16561 aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg aagctagatt
    16621 tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa
    16681 ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat gaagtagaaa
    16741 aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg
    16801 gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt ttctatgctg
    16861 aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg
    16921 aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct gaaggattta
    16981 cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg gatgtctggt
    17041 ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta tcattcccag
    17101 cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca agtatcacga
    17161 ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa ggatttgctt
    17221 tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca ccagaaaaag
    17281 tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta gattatgaag
    17341 cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat cgtaaaatca
    17401 gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa aaagatactg
    17461 tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg aaccaagtaa
    17521 tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt taccgtacag
    17581 ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct aaagatgtag
    17641 tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa
    17701 cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca gaagaaatct
    17761 ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct ggttatgaag
    17821 agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac ttccgtgata
    17881 atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa tctttggaag
    17941 caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag
    18001 atattgcaca attattgatc gtttcaccag actactttga agttatggca gcagatgaac
    18061 aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa aaagcagacg
    18121 gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat gaaaaacttc
    18181 ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg
    18241 aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg tcacaccccc
    18301 ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg ttgccagatc
    18361 cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat tgatccagct
    18421 ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac atccacgata
    18481 tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc attcaacgca
    18541 tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc actatttcta
    18601 aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc attcaaggat
    18661 aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg aagtgtcgta
    18721 tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg tttgaatacg
    18781 atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca
    18841 cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc agtctctgat
    18901 tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc caattcacct
    18961 tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata aatgcggata
    19021 gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc tactggcggt
    19081 tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg
    19141 ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc aaaagtaatt
    19201 tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt ggcaaaacgg
    19261 atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct gaaaatatct
    19321 tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg gtaatcagaa
    19381 ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat attttgtgat
    19441 ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata atagacacgg
    19501 ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa tgtctgataa
    19561 tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa ggtagttgct
    19621 tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg gtagtgctca
    19681 tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa atttcctttt
    19741 ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg
    19801 aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt tatattttat
    19861 cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta tcattttgga
    19921 tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg ttgatattca
    19981 ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt gcgatacgca
    20041 cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg acaacttcct
    20101 cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa tagttttcta
    20161 aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca tcatggactt
    20221 cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa
    20281 tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga taaggggaat
    20341 gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat gcagctgaga
    20401 catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca cctagttcta
    20461 aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa
    20521 aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt ctaaataaaa
    20581 aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag aaga
//
LOCUS       DARDHD010000034        20272 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000034 DARDHD010000000
VERSION     DARDHD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.034"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1509
                     /locus_tag="SDA06_000982"
     CDS             202..1509
                     /locus_tag="SDA06_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG4228275.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1630..2829
                     /locus_tag="SDA06_000983"
     CDS             1630..2829
                     /locus_tag="SDA06_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4228276.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2856..4124)
                     /locus_tag="SDA06_000984"
     CDS             complement(2856..4124)
                     /locus_tag="SDA06_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG4228277.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(4294..4935)
                     /locus_tag="SDA06_000985"
     CDS             complement(4294..4935)
                     /locus_tag="SDA06_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG4228278.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(5344..5847)
                     /locus_tag="SDA06_000986"
     CDS             complement(5344..5847)
                     /locus_tag="SDA06_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4228279.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(5970..6734)
                     /locus_tag="SDA06_000987"
     CDS             complement(5970..6734)
                     /locus_tag="SDA06_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG4228280.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6842..7117
                     /locus_tag="SDA06_000988"
     CDS             6842..7117
                     /locus_tag="SDA06_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG4228281.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            7677..8627
                     /gene="yidC"
                     /locus_tag="SDA06_000989"
     CDS             7677..8627
                     /gene="yidC"
                     /locus_tag="SDA06_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4228282.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(8673..9074)
                     /locus_tag="SDA06_000990"
     CDS             complement(8673..9074)
                     /locus_tag="SDA06_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4228283.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(9088..9897)
                     /locus_tag="SDA06_000991"
     CDS             complement(9088..9897)
                     /locus_tag="SDA06_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4228284.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(9884..10774)
                     /locus_tag="SDA06_000992"
     CDS             complement(9884..10774)
                     /locus_tag="SDA06_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228285.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(10787..11266)
                     /locus_tag="SDA06_000993"
                     /pseudo
     CDS             complement(10787..11266)
                     /locus_tag="SDA06_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(11442..12383)
                     /locus_tag="SDA06_000994"
     CDS             complement(11442..12383)
                     /locus_tag="SDA06_000994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG4228286.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(12380..13375)
                     /gene="lacD"
                     /locus_tag="SDA06_000995"
     CDS             complement(12380..13375)
                     /gene="lacD"
                     /locus_tag="SDA06_000995"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4228287.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(13368..14564)
                     /locus_tag="SDA06_000996"
     CDS             complement(13368..14564)
                     /locus_tag="SDA06_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4228288.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(14582..15310)
                     /locus_tag="SDA06_000997"
     CDS             complement(14582..15310)
                     /locus_tag="SDA06_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4228289.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(15751..16725)
                     /gene="lacD"
                     /locus_tag="SDA06_000998"
     CDS             complement(15751..16725)
                     /gene="lacD"
                     /locus_tag="SDA06_000998"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4228290.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(16722..17180)
                     /locus_tag="SDA06_000999"
     CDS             complement(16722..17180)
                     /locus_tag="SDA06_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4228291.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(17177..18640)
                     /locus_tag="SDA06_001000"
     CDS             complement(17177..18640)
                     /locus_tag="SDA06_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4228292.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(18622..19554)
                     /gene="pfkB"
                     /locus_tag="SDA06_001001"
     CDS             complement(18622..19554)
                     /gene="pfkB"
                     /locus_tag="SDA06_001001"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4228293.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            19696..>20272
                     /locus_tag="SDA06_001002"
     CDS             19696..>20272
                     /locus_tag="SDA06_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4228294.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6097 a         4378 c         3421 g         6376 t
ORIGIN      
        1 tcagtttatt tgctttcagc gcttcccgct tattagatgc ggaaacacag cctattcctg
       61 taaaagtacg taaaaacgac cgctaacctt ccataacgtg cgttcttatc ctaaagtttt
      121 ttcagaaaca ttgaaactct ctcccctctc tgataaaatt ttaatttgta gcgaaacaaa
      181 ttaattacaa ggagatcgga tatgtccata aaaaaaacac gtctagtcct attattggct
      241 agtctcattc tttgccttgc ttcttttcta ccagctgttg ctgtgcaagc tgaagatact
      301 ttcaaagtaa atgcaaaagc agcctttgct gtagatgcgc aaagcggtaa gattctttat
      361 gatcaagatg gagaaaaaac catggggatc gcttcaatca caaaaatcat cggtctttac
      421 atcgtccttg atcaagtaaa agaagggaaa ctatcatggg atgataaagt ttcaatttca
      481 gattatgcag aaaatttgag tgtcacccca gatttatcaa atgttccttt gcataaagaa
      541 aacacctaca ccgttaaaga attattcgac tctgcaatca tccagtcagc caacgcttca
      601 atggttgcct tggcagaaaa aatctctggc agcgaagcaa aattcactga acgaatgaaa
      661 gaacaattga aagattgggg tatcaaagat gcaacgatcg ttaatgcatc aggactgaat
      721 aactcttatc ttggcgaaaa ccgaccagaa ggtactggag aaaacgacga aaatcaaatg
      781 tctgctcaag atgtggctat cgttgcccgt catttgatct tggattttcc agaaatactg
      841 gatgtatcta gcactacgac acaaatgttt ggcgaaaaca cgcaatcacc agtagaaatg
      901 gtcaactgga actggatgct cccaggattc gtgaattata aagaaggcgt ggacggatta
      961 aaaactggta cgactgaact tgcaggagcc tgtttcgtcg gtacgatcac taaaaacgat
     1021 caacgaatca tcactgtcgt attgaatgca gacggccatg cggaaaaccc aagtgcccgt
     1081 ttcaatgaaa cagctcgctt aatggattat tgctacgaca attggtcaga aaaagagctt
     1141 ggaaaagcca atgcaagtat ccctagtcta aaaaccattg atgtaaaaga cggaaaagaa
     1201 tcaacagtgc ccgttgtgct taaaagtcct gtaaaagtat gggtacggaa cgatatggat
     1261 actggaaaat taacaatcac accaactctt gacaaaaaac aagtcactga caatgagatc
     1321 caagcaccta tggataaagg aacaaaaatc ggtaccgcaa cgatcacctt ggcagaagat
     1381 aaattaggtt atcttgaaga caacaacgcg ccatccacag aaatcatcac ggataaaacc
     1441 gtagaaaaag caaatatttt cgttattggc tggcgacgcg tcgcagaatt tttcggtaac
     1501 ttgttttaat tgaacaaaaa gaaaaaagca tctttccatg tggaagatgt ttttttgctt
     1561 tttctagttt aaataacagc ttttttcatc tattttcgtt atactgtata agaaaaagga
     1621 gttgaattta tgaatctaac cagagatctg atttttttgg ttctctatct tattggggta
     1681 ttctccatcg tttattggcg aaaacagaaa aaggatcctt ccttgttctt ttacagcagt
     1741 acatggacgt ttgcggtaat cttgacttat tttgtcgtgt tggaaatttt ttattcgacc
     1801 ttttattttt tgattccctt agtgtttttc agtatctttg ccttttccta tttttcagaa
     1861 aagcgaaggc tgttgaatgg tcttttattc aatattttcc tcattagttt tggtatctac
     1921 ttgtttgtct tgctatacga aacacaaaat atttttcttg gcggactgat tgccttgatc
     1981 acgatcccgt tactgcttgt ctttctattc ggcatttatg gattgatcgt ttttcttttt
     2041 tggaacggtg tcacagtttt acgtagagag tcgcattcat tagctaatct tttaacgttg
     2101 atactagcca tttttcttac attgtttctt gtttttgact ttttcttgtt aaaatattta
     2161 ccacaatgga taaatgcttt gtttttctgc atcccattga tcttgatcta tctatttatc
     2221 gtattctaca actttttgac tgtctcattc ttgtatcagt tcaaccgccc tcgatacaac
     2281 caagacttca tcgttgtgtt aggcgccggg ttgatcaatg gagaaacagt ttctcctctt
     2341 cttgccaagc gaatcaataa ggcgattgct ttttattggg cacaaagccg tgcgacatta
     2401 aatccgccta tcctattaat gtcgggtgga cagggagcag acgaaaaagt accagaagca
     2461 atggcgatga aacaatatgc catggaacaa ggaattcctg aaagagatat tcttgtcgaa
     2521 accaattcga caaccacttt ggagaatatg cgctattcaa aggaaatcat ggatcagcaa
     2581 atgaaaggtc cctatcgcgc gatcttcagt tccaacaatt accacatttt cagagctggt
     2641 ctctatgcac gacaagcgaa actgaaggca aatggcattg gggcaaaaac agctttctat
     2701 tacttgccta acgcgttttt aagagaatac atcgccatct tgatgatgaa caaaaaaaga
     2761 catatgatca tttgcggatt gcttttcgca ggttccgcat tcttatcact gatcacttta
     2821 cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca ccttgttttt ttcttttatt
     2881 ctcttgacga ttggcaagaa tagtattcct tttttgacgg ctacttgctg tgcagaataa
     2941 ataccgctgt ttcctgaaca cataaagctg atcaagcaga tcataaagaa gtaagcagca
     3001 gcttgtgaac caaataattc gattcccatc acaaaacagg caatcggtgt atttgtcgca
     3061 ccagaaaata ccccgataaa acctaatcca gcaagaaaag gaacagaaac atggagcaaa
     3121 ggcgctagta ctgcgccaag cgttgcacca atctcaaata gtggtgtcac ttctccccct
     3181 tggtaccccg cacctaaaga caagaccgtg aagaataatt tcccaataaa atcataagca
     3241 tgtgcctgtc cattaaatga ttcctgcagt aacggaagac tcaacccaag gtaacgttgt
     3301 gtttgcaaaa tcaacgctgc ggctacaact actgctccac ctatcgtatt tcgccaaaca
     3361 ggatgaggaa tccattgcgc ataccgcgct tttaaaaaga cgatcgaacg gctgaatacc
     3421 caaccaatca aaccaaaagc aatcccagca acaaaaactt tgaagaacag caacatactc
     3481 cattcaggaa tcacacccat ttggtaatgt agatgagaaa caccatagct ttctgtcatg
     3541 aaatttgcaa aaagcgcagc aaagaagcta ggaaatattg cttctgttcg gactttaccg
     3601 attgccaaca cttctaatcc aaataatgtt ccagctaaag gtgtgccaaa tacagaacta
     3661 aaacccgcac tgattccact gataaccaaa atctcccgtt ctgctttatc caatcggaat
     3721 aaatgcgcta tattatcagc taatgctccc cccatttgaa ctgctgttcc ttcacgacca
     3781 acagatcccc caaataaatg ggtagtgatg gtcccaaaca aggtcaatgg aatgagccgc
     3841 aaagggatct tctcttctcc tccattccct tgatcgatca ctaagttatt cccacgacta
     3901 gaaagaccgc catgatacgc ataaagataa gcaaaaagcg cgccgctgac tggcaataga
     3961 taaagtagcc aaggatgtgc tagtcggata tctgtcacga acgtcaggct atttaagaaa
     4021 aaagcagaca agctccccat gaatactccg agaatcgctg tgaccaccag ccacttcaac
     4081 atgtacatca aaatacgcac tggactcggt aattgttttt ccataaaaaa ttcctcctca
     4141 aataaaaaaa cgcctactaa agaaaggggc tggccccaga ctttagtagg cgtcattagc
     4201 tatcttacgt tagcatttaa aggcgaacac catcacctat tcacttccat taaaagatag
     4261 catatttttc tgtaaaaaac aataaaatta tcgttatttc tttacacgtt cagcaaattc
     4321 atagaaagga gccaatttcg ttaactgttg attacattga tcactatcat gacaaatata
     4381 attccccttt ttcgtatacg tgccatcaga accggctttc gttgtagaaa gaaagagcga
     4441 gacattcgat gttttctgac agatcgcaca gattccctta ttaatcgttg gtgataaagt
     4501 accgctgatc ccttttaatt ttccatcttc gtaataaacc atgaattttt tttgcgtccc
     4561 tggatcattc caaccgatgt acgtatgtgc ttgcaagtcg atttcattcc aagcgggttg
     4621 tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctcg gctgcataaa
     4681 cggctggacg atttgcttca gctcctccaa tgctcgttct gcttgcgttt ttgtcagttt
     4741 tacatctaaa actgtcgtta aaaacgtatc gatttctgat ggttcgtaac ctaatacatc
     4801 gcgaatcttt tcagctgcta gagcctgaac cgtttgtacc gtttttgagt cattgacaga
     4861 tgagtacgca tttaataact gctctacttc tctttttata aaaaaatatt gatatggttt
     4921 cattgtttgt tccatgattt taaagtcctt tcttctaacc aaagtgcata ctttgtagaa
     4981 aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc cggactcttt taaataagat
     5041 acgttttgca tcttatcatc acccccttta atgttcattc ttctatgtac atgattcatt
     5101 cgataaataa aagtaaaccg tatcttgagc agatagtcaa ctgcaaacag aaaaaggact
     5161 gtgacaaaag tcgtgtcaaa gtccctttct acaaacaaac ggtgttccag aagtagcttc
     5221 ttcaaaaaat aagccggaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
     5281 tatttcttga agctgaccca cttctgtcac aacctcaata tggtctatta ttggttttac
     5341 ctgttaaatc gaggattctt ttccgaagta acgttgccat ctcttacgca tacttgctga
     5401 cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta cgatatatcc
     5461 ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa tgatatctcg
     5521 ctcaagatcc gacaaatcgg tgatcttttc tgcattcttt acagcgattc ttggatggat
     5581 atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga acagatcatg
     5641 caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata
     5701 actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta
     5761 attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag acatatattc
     5821 ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt
     5881 ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt cgccttggat
     5941 aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag aacaatccct
     6001 gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata tacgttctga
     6061 tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat gattattgcg
     6121 aagttatctg atttttctac tttgtctaag gcgtgtgctt cttcatttaa ttctgtgcca
     6181 tacactgtaa accctttgtc tttcattaaa ggaatcactt ctgtcaaatc tttttgcaac
     6241 acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact ataaatatct
     6301 gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt acggatcatc
     6361 gtccctacat ttccaggatc ttgtacatta tctaataaca gccatcctcc tgaaaaatct
     6421 ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt aggtagatcc
     6481 gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt gtttgcggcc
     6541 agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt tatttctgca
     6601 tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc ttccttttct
     6661 tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt ttttgctgat
     6721 tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg tgcttgagct
     6781 tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa aggtgacgaa
     6841 catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat ttcgctacat
     6901 gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg aagatgacgg
     6961 ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta ttgaagaagt
     7021 caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag aagacccgat
     7081 gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag ctggtctgca
     7141 tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca ggctggactt
     7201 aatgccgtag atgcaagctt ttgaacaccg tcaataaggt tgtgagaaag tcgcttagtt
     7261 ttgagtagta aggagaattt tgtgaaaatg gctttccaca tttttgcata aaatttgact
     7321 taataccgag aaactgactt ttgaacaccg tcgataaggt tgtttttgag acgctttgcc
     7381 tcaagtatta aagataggta gtaaaaaagc tgatctataa cttgcaataa atttgcttaa
     7441 cccatatttt ccagacagaa caaaaaagtt tttagttttt taaataagaa agaaacactt
     7501 ggaatcactc ttttctttcc aagtatttct ttcttgattt tttgtctttt tttcaaaatt
     7561 tttcgctacg taaaagaacg aatatttata tgaaatttct atgaagttga ttgaattcca
     7621 tgcgcttttt tagtatagtt aagtttgtgt aaaaattata gaagaggaag tattctatga
     7681 ataaatggaa aaactggtta ttgggatccg gacttgtcag tatcctgctc tttttatctg
     7741 gctgtgtgcg gatggattca aacggaaacc ctgacacttc gggaatcgtt tatcgagtac
     7801 tcgtccatcc tatgggccaa gcgattacat atctagttga gaatttcaat tggtcttatg
     7861 gctgggcagt tattgcaatg actgtgatcg tccgtatcat catcttgcca ctaggtatta
     7921 gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca agcgttaaaa ccacaagttg
     7981 aagcagccca acaaaaattg aaagctgcaa caacacgtga agaacaaatg gcagcacaag
     8041 cagaaatgca gcaagtatac cgcgaaaacg gattgagtat gaccggcgga atcggctgtc
     8101 tgcctctatt gatccaaatg ccgatcttct ctgccttgta ctttacggca cgttatacag
     8161 aaggtattcg ggaatcatct ttctacggca tcgatttagg ctcaccaagt atggtacttg
     8221 ttgtcattgc aggggtcgcc tatctgcttc aaggatacat ctcaacgatc ggtattcctg
     8281 aagaacagaa aaagacgatg cgtacgatgc taatcgtctc tcctgctatg atcgtcttca
     8341 tgtcgatcag tgcgccagcc ggtgttacac tttactgggt agtcggtggt gtgttcagct
     8401 gtctgcaaac attcatcaca aacgttatga tgaaaccacg tttgaaagca caagtggcag
     8461 aagagttgaa acaaaacccg ccaaaacaag tcgtcactcc aagaaaagac gtgacagaat
     8521 cagcaaagcc agctaacaac aaacaagcaa caaaacaaat caaacaacca aaaaacagta
     8581 caaacaacaa aggccgcaac gcaggaaagc agcagaagcg gaaataaaaa cataggacaa
     8641 aaaagatgag agctaagccc tcatcttttt tgttactctt cctcttctgc tattccatta
     8701 attaattcgt tgacagagac aatgctttca catcctgcac gaacaaactc gggctctgaa
     8761 ccaatcattt gtgagttgat aaattcaatg accatcggaa gattcatccc agcataaaga
     8821 gtaatctgtt gtccttccat tattagttta gaaatgatat ttgcaggtgt accacctaaa
     8881 agatcgcata gtaccacaaa atcatttaac gaatctgtga cagtgagaaa cttttgtttg
     8941 tatgtttcag tgccatcttc tggtaataaa ggtaacgtgt agatatcctc ttgaggcccc
     9001 atgatcattt ctgtactttt tttcaattcc tcacaaaatt ttccgtgact aatcaatacc
     9061 aattgtctac tcatttttcg ctctccctta tttcgaaatc acacccaatg cagataaagc
     9121 aacacacaca attaatacga taaaaatcgc tcgagtagga gtcatgtttt tctttcctaa
     9181 taaccaataa acaccaccga cgatcccagc aggtaaaatt ttaggtaaga tcatatcgat
     9241 atagttttgc atattcaatg tcacatcgcc aatcgttggt gcccatgaaa atttaacatt
     9301 gatcattgtc gcaactaatg cccctaccat gaatacccct aataaagtgg ctgcgtccgt
     9361 taatgcagtt aagcgagatt gcatagtagt aactaaggaa attccttctt tgtatgcaaa
     9421 ttcaagttgc ttccaacgaa aaaccatgac aacgatctga gcaaaaatcc atataaacac
     9481 acctatcgga tttccatcag ttgccatatt cgccgccaaa gccccaaaaa tcgttggaat
     9541 caaagcagca aatacggaat ctccaatcgc tgcaaaggaa cccatcaatc cagctttcag
     9601 ccccgatacg gtttcctttg aagcaattcc ctctttttct tccattgcaa gatcaatccc
     9661 agtaatgatt gtattaaaaa aattagaagt attaaaaaat tgcgtatgtg ttttcatcat
     9721 ctcttttaat tctggtgtat tgtctccata tatttttctt agttgtggca agatagtata
     9781 taaatagcca gaaccctgca ttctctcata gttccaacct aattgaaaag tgaacaaact
     9841 tcttcgattg atttgcttaa aatcttcttt tgttaatttg taattagatt tcgtcatctt
     9901 cgatttcccc ctcaacagaa tgtgatggct cactagctaa tggttgatgg tctttgtttt
     9961 gatcattctt ataactgatc attgctagtg ctagaccaat caatgctaca gcaagcatcg
    10021 gcagcgaatt gaaagttgcc ggaagatctt taacaacgct tgccacactt gttcctaata
    10081 attgaacatt agagaataac gttgctaata atgccgtgat cgtaaatcct aaaataagat
    10141 aagatagatg tttttttact ggtaaatagc gcagaagaat ggcaaaccca acagctggta
    10201 aaacagctcc tgctactgaa agtccatcgc ctagccattt tagattcccg tttaaaacag
    10261 aaacaacatt ttcaactaat ccaccaccaa atgctaaagc taaaaataca ggaatcatac
    10321 gagagagcga ccaaggtaaa gctcccatta agaaattgcg ttcgattgct tgataattca
    10381 tgtcttctac gtgacggtca atacgatggg caaagtatgt gttagcgaat ctagctaaaa
    10441 tatccaattg gatcattaag cttgctacag gcactgcaat agcagcaatc gcttgttctg
    10501 gattcattcc aagagaaatc gagaaggcag ttgctaaaat cgtcccagag ttggcatcta
    10561 tttttgatgc gcctccaaac gtccccactc ctaaaactgt caactgcata ctgccaccaa
    10621 taatcaaacc tgcgtttaaa tcacccatta ctaaacctgc atataaccct gcaaacactg
    10681 gcgcactcat tgaagaatag atctgtaatt catctaaaat ttgataacct gcatataatg
    10741 ttaataataa gatctgccac cattgaatat ccatggatca ttcctcctat ttgattaaaa
    10801 gattcataaa gtcttctgct ttatcgtttg gtaccatttg agcaattaaa tgtataccaa
    10861 gctgatctaa ttttttaaat acttcgatat ctgcatctac cacattgatt gattttgtaa
    10921 tggaccgtgt ttcattcgtt tgtgacatat tccccacatt tacttctttg ataggaactc
    10981 ctttttctac taaagataag attcgatctg gcttttttgc tacgatcaat actcgttgag
    11041 agtcatattt gccagctaaa atatttgttg ctgctttttc gattggcaaa atgcttaatt
    11101 ttacccctgc tggagtcgct aacttcaatc cacttttttc aattgtgttt gtggctacct
    11161 catcatccac gaccatgatt cgactaatat tcagtttcgt tgtccaaaga tttgctactt
    11221 atccatgtat caaacgtcca tcgattctta caccaataat tgccatcatg cattctcctt
    11281 atctattttt tctatttatt gttcaattaa aactgtgaca tcatcaaata tcccttctgt
    11341 ttcaaagata acgttttcat tttttctttc ttatatttca tttttaagct aagaacaaat
    11401 tccttcccaa ctaaagaaaa cgctttcttt tatatttaag ttcatttgat tttcttaact
    11461 tgaatgtgtt tcatcaacat cgtaactttc atagggtcga tccagccagt tttctcttgt
    11521 tgcgcgtttg ccataccaca agccatagaa aatgcgagaa tctcttcgat tgattgcctt
    11581 tttgataacg catgggccga tcctgcaagg gtcgcgtcac ctgaaccaac aggattaaca
    11641 actgatattt taggaataat gacttcgtaa aaacactgag caacttttgc cacagcacct
    11701 ctctctccta aagaaacaac aacccatggc actccttcaa atattggatg agctaataag
    11761 gactgaattt tttctaattg aatcgaagaa cagatacgtt ggccaatcag ttctcctaat
    11821 tcttccagat tgggcttgat aagtagtggc ttctccttac cttccaatac ttcaagcaaa
    11881 aatttaccag aagtatctag taatatgtct ttcccttttt tatcagctaa agcaaccaat
    11941 tgcttatagt aatcaagagg gcatccttgg ggtaaactac ctgagatcgt cataatatct
    12001 gatatttcga ggagctgctc gaaatgatcg gtaaatgtct gttgttcttt ctttgttatg
    12061 aaggggcctc gttccaaaag ctctgtttgt ctcgaactat gcaggatagc aatagaatcc
    12121 cttgtctctc ctttaattgg aacaaactga tgcatgatcc ctaactgatc tagctggttc
    12181 tttagatatt caccgtgctg gccacctatt aaaccagtag ctatcacctc ttctcctaat
    12241 aaatttagaa ccctcgccac attcactcct tttcctcccg ctgttttatt gactgtttca
    12301 actcgattga ctttgtcgag gtttaattct tcaattgtat acgcgatatc gatggaagga
    12361 ttcaaggtaa ctgtcgtaat catttcatct tcaccttttt gctttttgtt ttattgaacc
    12421 aaggggtagc ggtttcattt attactttat ttaaagtctc gatgttttct ctcccatttg
    12481 ttcttaacca atgaatagct ccgtcttttc cattttcgac gtatgctttg accccatctt
    12541 tccatgtcgc acgtccacac aatacaccat tgaaggtgga acccgattca tgagcaaaaa
    12601 tcagtgtttg ttgaaataat tctgttgaaa cacccccact caaaaaaatg aatggtaatg
    12661 gagtcgcttc actttgctcc ttaaagaagg cagcagcctc ttctttgctg tgaataaatt
    12721 cacctgtgga atagccattc acataattca tattgactgg tacttctact ttcaacacat
    12781 caactttata ttttggatca gaaaattctt tcatcatctc aataactttg tggggcttga
    12841 ctttcgcata ctctggagaa gaactatcgc ttatttgtgc atcataagaa actaactcca
    12901 aatagaaggg acgattttct ccttcgcact cactacctaa acgttcgatg aacacatgct
    12961 tcaaatgatt gatttcgggt gcttcatcaa catcgtagta aagtaagaat tttactgcat
    13021 ccgcttgctg ttctttcaat ctgagagaag accaatccgc taataaatca ggtagacgac
    13081 caggcgtagt tgcgtcatag ccggttttct cataagctaa caataacccc gtctcttttg
    13141 ctctgacttt tgctgctggc aatccatatt ccggatccaa taaaatagca gaagcatagg
    13201 gtgtcagctc agctgatact agttctttga attcactgat ttggtcttca cttggctctt
    13261 ttcccaacga tttgatcatt ttttttagag caccacgctg atcgatcgct aatgcactaa
    13321 taatcccatc ttttgttgat agtctctcca ttgccctatt tttttcttca ttcatctgtc
    13381 atttctcctt cggtttcata cttatgaata atgacgccct tgactactcg attgaccgtg
    13441 cctgttggag atggtgtatc tggtttatta gctactttga ttgaagtata gagtgcaatg
    13501 atttgtgcag tcataatcat cgctaatgca agataggcat cgggcaattt actcaccgag
    13561 tcttccaata gcacagtcat acctgagaaa tcaatagtac ccggttgcgc aatcgcaatg
    13621 acattggcag tgacccgatc ttcttttatt tcctctaaaa tatctaagtc ataatttcgc
    13681 gtatatggtt gattgctcac aaaacctaca acgagtgtct cgctatttaa aaaagacttt
    13741 ggtccatgac gaaaccccat cgaagaatca aaaattgtag caactttacc tgctgttaat
    13801 tctaaaattt ttaattgtgc ttctcttgtc gcacctgcca aacttcctga acccagataa
    13861 gcgatccgtt caaaaaaatc aggtaataac ttttgaattt cttgatctct tgaaagaaga
    13921 tcttgaccga ttttaactaa ttgttcaaga tatctttctt ttttctccat tgctgtttga
    13981 tcaaagatcc ataaagcact caataacata cacgtaaaac ttcctgtcat agcaaaacct
    14041 gcatcgtttg ctctctctgg catcaataac agataattct gtttatcatc ctgtgcgttt
    14101 tttgccaaca tcccctcttt agcacaagta atcgtcaaat gaaacaatgt ttcaacttgt
    14161 tcatttgcaa tttgcacagc cgctacactt tctggactgt tgccactgcg agcaaatgaa
    14221 actaataaag tcggttcatt tggaaacaaa aattcttttg gtgaagctac aatatctgtc
    14281 gtgccaaaac tgtggaagat aaaaaactga ttgtctccat gttcatttag atagggtaag
    14341 agcgtatccc caatatattg agacgttcct gcacccgtaa aaataacacg tattcttctt
    14401 ccgtttgctt ctttttttat ttgatcaaaa aatagagcta aattttcttg gctttgcatc
    14461 aatctatcga ttgtttcttt ccagagatct ggttgttgtt ttatttcttg tgtcgtgatt
    14521 gctgcaccta tttgttttaa tttgttcttc gatagctcaa acatgttgtt ctccctctca
    14581 atcattttcg gatatgccga attttataat gaaactggtc cgctcttgct acactatctg
    14641 tatattcgat aatctcattt ttcacgttat aggttatcct cgtcagatgt aagacgggag
    14701 cgccatcagc gattcgtagc acaacagcat cttttttttg cacgatactt gcataaaact
    14761 cttcatcagc taattgaact tgttgttcat aatcctgtga aaacacatca tacaacggtt
    14821 tactttcaag taacggcaaa tcaagtccca taaatagttt cgccggtaga taagaacgct
    14881 caaccatcaa tggctcttca tctgccaaac gcaatctttt aagtttaaat acagtttcac
    14941 ctaaatggat gttcaaattc ttagcaattc tcttattagc ttccatttgt ctaaattcta
    15001 aaatgaccgt cttagggacg cgccctattt cgcgcatttc ttctgtaaag ctatatgctc
    15061 cagctaagtt cgatgactta tcagacaaat cagagacata ggtacctttt ccatgtcttc
    15121 ggtaaatata accttgattt tccaattcct gtaaagccag acgtacggtg gtacgactca
    15181 ccccatagtg ttgacaaagt tctctttcag ataacagctt atcatgcgga gtcatctgtg
    15241 tttcgatacg atccttcaat aaatcaacta gttgataata caatgatttt cctttatttg
    15301 agctctccat aaaaatcacc tcttctttac tggttataac cacaacacaa gaatagtgaa
    15361 ttattaatca tttgtcaaag gttttttaga ccaaaaaagc tattataaca gctctttaaa
    15421 gctttatatc ttgaatagac aaagatacct caaaaatacc agttaccaat aaaagaacag
    15481 aaaaaaagac gaaccacata agcgatccgt caaaatggtc taacttatcg agcagcgtga
    15541 tccacattaa tccctatgat attcctacaa ctatcgtttt attacttcca atatcattta
    15601 caacaactaa aaatagctta ttaatgacaa atgttattat tagttttaga taacttttgc
    15661 agttttatac tttttttcta taaaatttga caaacatttt tttaaataac taattatttt
    15721 ttgaatctgt cactcagaca atcctttctc ttacttgtta acataaatag aagttgctgt
    15781 ttcttctaat agtgtactta attctttaat attttgtttt ccttcttcta acatccaact
    15841 actcgctttt tcttcgcctt cggtaacaaa aggtgctact cccttcgccc atgttgctcg
    15901 cccacataag acaccattga atgtcgaacc tgattgtttt gcaaaacgca atgtatcttg
    15961 aaatagtgca gcgctgactc ccgcacttaa aaaaataaac ggttgttcag tcactttcga
    16021 ttgttcatag aaatacgcgg atgcctctgc ttgactataa caagtatctt gcccatatcc
    16081 ttccacaaat ctcatattca ctggtacttc tactttcaat acatccactt gataacgttc
    16141 ttttgaaaat tctcgcatgg cttcaatcac tttatgcggt tttacttttg catagtcaag
    16201 agaagacgga tcgccaatag ttgcatcgta agaaaggagc tctaaaaaga aaggtatgcc
    16261 ttctgcttcg cattctgaac caatccgttc tataactatt tgtttttgca cattaatctc
    16321 tactggttca tcaacgtcat agtacaataa aaatttacaa gcatccgcac ccacttcttt
    16381 taaccttttc actgaccaac tattcagtaa atctggtagt cggcctggca ttttcgcatc
    16441 atagccggtt ttttcataag ctagcaacaa accacattct ttctctcgaa gcgctgctgc
    16501 aggcagacca tattctggat cgagtaaaat agcggaagca tacggagtca ggtactgaga
    16561 aaccatctgt ttaaattgga tgatatcttc tgatgtagct tctttatatt ggttgatcat
    16621 tttcttcaaa gcaccacgtt ggtcgatagc tagcgcgcta ataattccag cttcatccgt
    16681 caatcgcttc aaacgttcct gtttttctat actcattttc ttcatatatc ttcacctatt
    16741 tctttagtta atattgtagc tatttctatt tttgattttg cagctctcaa ctgttctgta
    16801 acctcttttc gcatcaatga tcgtgcaacg agagacaata atttcaaatg agtcgttcct
    16861 gcttctgaat ctgggataaa taacgcaaaa ataaaatcaa ctgactgtct atctaaactg
    16921 ttccattcaa ttcctttttt cagtgtgacg ataatcaaac taggttcatt gattgtttga
    16981 gacttcgcat gagggatagc aaaacctgac attattcctg tagtaccttc tttttctctt
    17041 tccagaagtt tttgatatac ttctttttcg tcatgagcga ttcctaaaca gaatgtttgc
    17101 tttgctaaaa acttaaatgc ttcttcttga ttatctaatg gctgttgtaa aaaaatgttc
    17161 tcttcatgaa tgattgtcat gataaacctc ctgctcttcc ttcttcgata actgtacgat
    17221 ttgttgcttt attatccctc ttcttgtctt tatcagttac tcttttcttt tggatcaaac
    17281 ctagtatcac tccaccaacg atcgaaccaa taagaatcgc tgtaacccat tggatttttc
    17341 ccgtcactac aggtaacact aaaaaaccgc catgtggggc tggtacttgg acaccaaaca
    17401 gataggtcaa gacagcagca attgatgagc ctaacatcat cgttggtaat actcggatcg
    17461 ggtctttcgc agcaaacgga atagctcctt ccgtaatatg agttgatcct aaaataaaat
    17521 taaccaaacc tgcattacga tcattagtat caaaatattt accaaacaat aaagtagcaa
    17581 aaccagtaat caacggtggt gcaatgcaag ctgctgacgc accagccata aaagtcgtgt
    17641 ttccttgtgt caaaagagct gttcctgtta catatgccgc cttattaacc ggtcctccca
    17701 tatcaaacgc acacatacag ccaacaatca cgccaagaac aagtggattc gaatattcaa
    17761 atccagctaa aaagctcatc attccttcat ttatagcctt cattggacct gccagcaaag
    17821 acatcacgat gccaataaat gctacaccaa taactggata taagacgatt gccttcaagc
    17881 catctagttg tttaggtaaa attatcagta gcttttgcaa aaataagata aaataacctg
    17941 ctaagaaccc agctaaaata cctcctaaaa atccagtacc gccattcatg gctacgattc
    18001 caccaacaaa tccaacgact aaccctggac gtttcgcgat cccttctgca atataagctg
    18061 ctaaaactgg aaccatcatt cccattgctg catcgtcaac tgcttttacc attgccgctg
    18121 tagcattgta ttgtgcatga tctggatcag aagaatagat tccccaaacg gcaaatgaaa
    18181 tagcaatcaa tactccgcca gctaccacga aaggcaacat atgagagaca ccgttcatca
    18241 aatttttata gatgctacga ccaaaattag caccacttat cgcttctggt tctaaagtat
    18301 cagcagcttc attatctgct aagacagtac cttttcctgc caatgcttcg tcaatcagcc
    18361 gttgtgcatc cttgattcct gtactgacag aaacatcaat gactcgtttt cccacaaatc
    18421 tttctgaatg gacatctttg tctgcggcaa taatgacagc atctgcctgt ttgatttctt
    18481 cagccgttaa ttcattctcg atcccaattt gtccatgggt ctccactttg atcgtgatgc
    18541 cttttttcct agctgcttgc tctaatgcct cttgcgccat gtaggtgtgc gcaattcctg
    18601 tcggacaacc ggttgctgca attagttgat attttcccat ctttcgaacc tctcttcctt
    18661 gattttgatt tgtttactta attcctccac atccaaaaaa tcagtcagtc ctttacgaaa
    18721 agcagtagag ctgcctgctg ctatgctttt acgtaacgtt tcatcaagcg atcgtcgatt
    18781 catgtatcct gctaaaaaag tgcctaacat ggcatcacct gcacatgctg tattgacaac
    18841 ttttcctttt ggcgagtttc ctcgaatcac cctgtctttc gtaaaaagaa tcgctccttt
    18901 ctcaccgagg gataacaaaa cattttctgc gcctgactct actagtcgtt gtccataaac
    18961 aaagtaatct tcatctgtaa ccatttttcg tccgaaccaa gaagaaagtt cttcctcatt
    19021 tggtttcagc aaaaacggtc gatagggtaa acaatccatg acttcttgat cactactatc
    19081 aataatcaaa caaatccgac tgatttctat taaaaccttt ggagacagcc ctcttggtaa
    19141 actcccagaa acacaaagat aatcccctgc ttgtaatgct cttatttgat ttaaaaaatt
    19201 atgaacagct gtctgcggta tttcaggtcc ttgattcact aatttgtatt cttctcccgt
    19261 ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt
    19321 ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtgaacc cagcagaaaa
    19381 acctaatgcc gtacttggga aatctagcat ctttaatact agtgacacat tcaccccttt
    19441 tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat gcatctcatc
    19501 tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa
    19561 cgacctcctt gattctatta tgaaatacgc gaaatagaaa atgttttctt aatcgtccca
    19621 acacaaagaa aacctttaca tttataaact cgtcgtgctt tttttaacta acttttggtg
    19681 aaagaaggaa agaatatgaa tcacaaatta agtgaaacag agcagtacct ctggaacttt
    19741 atcgaacacc tcatcctaga gatccctaac tattccatcg tgaaactaag tgaacaagca
    19801 aatgtctcaa cagccaccat tgtgcggaca atgaaaaaga aaggctatga agggtttact
    19861 tcgtttaagc atcatctaaa agaaggagaa aacaagaaca tcaatttttc atttttagat
    19921 aaagtagaca aaggaatccg ccgagccatt ctaaaaaatg aacaagaagt ggtacgtaca
    19981 atcaatatgt tggaaatcgg gaacattgaa gatgcaatac aaagaatcaa atttgctgac
    20041 cgggtttttg tctttgcccg aggattttca gaaatgattg gccaagaaat gctggtaaaa
    20101 cttcaattaa cgggaaaaaa ttgtcagatg catactgatc cagaaattat caaaagcatc
    20161 agtcggaaac taactaagaa agacagcgtt atttttgttt ctttgaacgg tgagaaaaca
    20221 aataaaaaca tcaaaaataa gatttatgtc tttatttttc aattttttaa at
//
LOCUS       DARDHD010000035        19841 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000035 DARDHD010000000
VERSION     DARDHD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.035"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            32..220
                     /locus_tag="SDA06_001003"
     CDS             32..220
                     /locus_tag="SDA06_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228295.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(864..1817)
                     /locus_tag="SDA06_001004"
     CDS             complement(864..1817)
                     /locus_tag="SDA06_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228296.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1840..2595)
                     /locus_tag="SDA06_001005"
     CDS             complement(1840..2595)
                     /locus_tag="SDA06_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4228297.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2592..3551)
                     /locus_tag="SDA06_001006"
     CDS             complement(2592..3551)
                     /locus_tag="SDA06_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4228298.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3544..4494)
                     /locus_tag="SDA06_001007"
     CDS             complement(3544..4494)
                     /locus_tag="SDA06_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4228299.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4644..5096
                     /locus_tag="SDA06_001008"
     CDS             4644..5096
                     /locus_tag="SDA06_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG4228300.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5185..7452)
                     /gene="gshAB"
                     /locus_tag="SDA06_001009"
     CDS             complement(5185..7452)
                     /gene="gshAB"
                     /locus_tag="SDA06_001009"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG4228301.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7639..8637)
                     /gene="ftsY"
                     /locus_tag="SDA06_001010"
     CDS             complement(7639..8637)
                     /gene="ftsY"
                     /locus_tag="SDA06_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG4228302.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8648..9457)
                     /locus_tag="SDA06_001011"
     CDS             complement(8648..9457)
                     /locus_tag="SDA06_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4228303.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9466..13047)
                     /gene="smc"
                     /locus_tag="SDA06_001012"
     CDS             complement(9466..13047)
                     /gene="smc"
                     /locus_tag="SDA06_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG4228304.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13067..13753)
                     /locus_tag="SDA06_001013"
     CDS             complement(13067..13753)
                     /locus_tag="SDA06_001013"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG4228305.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14101..15882)
                     /locus_tag="SDA06_001014"
     CDS             complement(14101..15882)
                     /locus_tag="SDA06_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228306.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15908..16822)
                     /locus_tag="SDA06_001015"
     CDS             complement(15908..16822)
                     /locus_tag="SDA06_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228307.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16849..17811)
                     /locus_tag="SDA06_001016"
     CDS             complement(16849..17811)
                     /locus_tag="SDA06_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228308.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17811..18755)
                     /locus_tag="SDA06_001017"
     CDS             complement(17811..18755)
                     /locus_tag="SDA06_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228309.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18756..19760)
                     /locus_tag="SDA06_001018"
     CDS             complement(18756..19760)
                     /locus_tag="SDA06_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228310.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5501 a         4265 c         3529 g         6546 t
ORIGIN      
        1 actctttgtc aatgaagata aaaccaaagc attggatatt tataaaaaat tagcttcaaa
       61 aaaaacgaat agttttggat tgatattaag cgagtataaa atcaaagcag aagagagtta
      121 tttctatcaa ttattaaaac ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca
      181 gatcatcaac tatcaaccgc ttgctgagac acatgcctaa atagaggttg tgaaatcagt
      241 gctttgagct aataccgaag gtcagctgat tgaacattgt ttagaagaga ttgtgacaac
      301 tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt
      361 ctagtgggct tattgccgag aagcagcaga tggaacatcg tttagaagag attgtgacaa
      421 ctgcgatttg ctttgagtaa taagaacgct ggacagaaaa tagcttctca tattttctag
      481 tctagtggac ttattgccga gaagcagcag atggaacatc gtttaaagaa gagatcggta
      541 caaaataaaa atatctaaat ttaagtttta tctaagtttc gctttgtacc tttgattcaa
      601 atcaaaggag gcgatcttga tgctgaaaaa atggatcaca ctattttttg ccactcccca
      661 ttctagaatc aacccgtatg atgtagataa tgaattcacg ttgaaaaaag atttctcaaa
      721 taaataaaaa ccatccgtaa aatcatcaat acgattttac ggatggtttt tgataacttc
      781 tttgaggata ggctcttgct atttaagaat gtaagaaaca ttattgttac atagtatgag
      841 agtttaatcc tctgttattt atgctattta aatgcttgat tcacatcttc gatcatcagt
      901 ttcattgatt ctaaaccgcc cccgcttaaa taccagacat ctggttcaag ggagatgatc
      961 tgctgatttt taccagcatt cgtttgtgct accagttcat tggcagaaat atcatctttg
     1021 ctgtcgtctc caccaatagc ttttgttctg tctactacaa ataagatatc cggatttttt
     1081 tccagcacat attcataaga aacactttga ccgtgagtgg atgcttcgat ttgatcatct
     1141 gcttgttcaa agccaaataa atcatggatg aaaccgaatc tagaaccaga tccgtaggct
     1201 gataattgcc cttcattcac aagtgtgact agtgcttttt tgtcagtagc ttctgctttc
     1261 tttttcgtct gatcgattgc actagataat tcctctaatt gagttttagc ttcttcttgc
     1321 ttaccaaaga tctcccctaa agcagtgaca ttttgctgga aagattccca tggatttttc
     1381 gcatctaaag aaaggaaaat ggtcggagca atttgactta gatctttttg aaaatctctt
     1441 tgtcggccag aaatgatgat tagatctggc tgaagctgat taattttttc taagtctggt
     1501 tcttttattc cacctgctga ttctactttt ttataggatg aaagatactc aggaaggctg
     1561 cttgtagctg ctccaactac cttatctcca acaccaagag catccatcgt atctaaagaa
     1621 ccattgtcga atacgacgac tttttctgga ttatttggca cctcgatttg gtctccatta
     1681 ctatctgtta tcgtcaatgt agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga
     1741 ctggtgttgt tgctatttcc acatgctgct aaaccaaatg atgctagaat caagatacct
     1801 actactaatt ttttcatctc ttctctccta atgttagttt tagaaataaa gacagaatct
     1861 gcggccttca atctcacaaa tccgtatgtc catttcgtat aattcatcca atacatcttt
     1921 ttttatcatt tcttgggtcg ttccttgcgt aaataactgt ccatctttca ttgcaacaat
     1981 ttcgtctgca taacttgcag caaagttgat atcatgaaga acaataacaa ttgttttccc
     2041 gatttcttgt actaaatttt ttaaaatctg catgatttga accgcaaaat tcatatctag
     2101 attgttcaaa ggttcatcca gtaaaatata atccgtgtct tgcgctagca ccattgcaat
     2161 atacgcacgt tggagttgac cgcctgaaag agtgtcaatc gattgatccg ctatttcttc
     2221 taacccaagg tgttccagtg attcctgaac gatttcctcg tcttttgtcg tcaattttcc
     2281 tttcgtataa ggaaatcgtc caaattctac taattctttc acggtgattt ggacatttat
     2341 ttcattcgat tgtcgtagga tcgataattt tttggctaat tcattggatt tccaagcctt
     2401 tacttcgtta tggtctaaat agatttcacc tgtatctttt ggtatcaaac gactgatcat
     2461 cgacaacacc gttgactttc cagcaccatt tggaccgata aaagcagtca atttcttttc
     2521 aggaatcgtt aactgtacat ctttaaccac tgattttcca tggtacagtt tattgacctt
     2581 ttgtaaatca atcataggat actcttcctt tcgcgaatca attttgtgac aaaataaatt
     2641 cctccgacaa attcaacgac gacactcact gttgtattcc agccgaaaat ctgctctact
     2701 gcgagctgtc ctcctgtcag aaacagcata gctaccagaa agcttccgat aaatagctga
     2761 ccatgacgat aagtagaaaa gacacgataa ctgatatttg cgacaataaa ccctaaaaaa
     2821 gtaactggac ccacaagagc cgttgatgtc cctgtcaaaa gactgacaca acataaaatc
     2881 aaaaattcaa aacgatctgt gtctatcccg aggtttactg cttgttcatt tccgagatgg
     2941 aaaacgtcta gcacatgtcg atatcgaaat aacaggtacc agcagccgat aatgatcaaa
     3001 gaagcaggca aaagatactg actctggata ttgccaaagc ttgcaaataa tctgccttga
     3061 agcaaatcat attcattcgg atccatgatg acttgtaaaa aggtactgac actaccaaaa
     3121 agcgtaccta aaatcatccc tgacatcagt aataaaaaaa gattcgctct catttttttc
     3181 aaaagaaaga aaaataacaa tagactcagc ccgaccatca gcccaacgtt caataagaaa
     3241 aaaactgtac ctgtctgctg aagcatcgtc actcctccga taaagaagaa aaggaccgtt
     3301 tggacaagga catataacga ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc
     3361 gtaattgtct gaaagctgat tgtggcactg ctgacagcta gcgagacaag gataaatgca
     3421 aaaattttct tactcctcaa tgcacatgca aaatgccagt ttccatatgt atggatcgtc
     3481 aagtacattc ctgtaacaaa taaaacaaga acaagcatac caataaaaat agatggcttt
     3541 tttttaagca tatcgtcgtc cccttatcag aagataaagg aagatagccc caccgatgat
     3601 tccaacgatc acactgactg gtatttcata aggaaaaatc aacacgcgag cgacaagatc
     3661 gcaaaatact aagaacaaac tacctgctaa agcagttacg ccaattgttt tgtgcacttg
     3721 atctccatat attctagaga caagattcgg aatgatcact ccgataaaag gaatgcttcc
     3781 taccatgatc aacatgactg cactagccaa tgatactaag ccaatgccta aaaactgcat
     3841 cttctggtaa tctattccca gatttactgc aaacgattcg cccagaccaa tgatcgtaaa
     3901 tcggtaagca aatagccaaa ccaagagcaa caatggaaca acagcataca ataactcata
     3961 atttcctcgt atcacagtcg caaaattccc ttgaagccat gaagtcatat tttgtaccaa
     4021 ctggaattga taagcaaaaa aagtagcaac tgaaccaatg atattcccta acatgactcc
     4081 aatcaacgga agcatcaatt gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag
     4141 caatgttcct gcaaaagcaa agcaaaatgc tgtcaaggat cggacaatca aggatgcacc
     4201 aggtaggaaa atcatagcca ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt
     4261 cgttggtgaa acaaatttat tttgggtcaa atgttgcatg atcaacccac aaatactact
     4321 ggtcgctccc acaaggatca aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg
     4381 taagctttgc ccatctaact ggagcagttc tgacaattgt acattcgata ctccgatgaa
     4441 caatgaaata ctacttaaaa taactaataa tgctactagt cgtccgtagc gcatccaatc
     4501 cacctttcta attgagaatg attttcattc ttattttaag tgaatcgttg aacaaaagca
     4561 accgcttttt catatgtttt aggtattatt gggaatatgc tataatcgta agaaagacaa
     4621 agagaagaag gagggtattt tttatggcac aagatgatgt aaaaagccat ttggataagg
     4681 gactttatgg tacaccttta gtgaaccctg aagaacagca taaatattta gggacattca
     4741 gggaacgctg ctatcttagt atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa
     4801 actttttaaa agaactcaaa aaacatccag atgcttccat actcttgaat ggggctctac
     4861 ccctttctgt ccaaaaccaa tatatccagc tagcaacgaa aaacaatagc cgtttcactg
     4921 tagtcaacga ttacgtcact gcaaatcctg aggaattcgg attgctctta acagcaaaag
     4981 aagcagtaaa tgaacctgtg attgatatcg aaaaaaaata cccagaagaa aactcttcgc
     5041 cagctgaaaa agaaaaacca aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg
     5101 aaaaaacaca taaattttta catgcaaaaa aagcttttga tcagattgac atcaaatagg
     5161 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
     5221 catcgtcaat cttctgcctt ttcctgcaaa aggataaaca tgcatatgca tggctggatt
     5281 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
     5341 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
     5401 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
     5461 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
     5521 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
     5581 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
     5641 tgttcggata ctgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
     5701 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
     5761 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
     5821 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
     5881 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
     5941 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
     6001 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
     6061 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     6121 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     6181 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     6241 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     6301 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     6361 aaccagcatt tgcttcattc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     6421 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     6481 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     6541 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     6601 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     6661 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     6721 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     6781 cagcgcactg ttgcgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     6841 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     6901 aaagtttctc gctgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     6961 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat
     7021 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     7081 atcttcgaaa ttttttaatt ttgcaataat gatctcttcg tctttttccg gtaaggcagg
     7141 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     7201 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacagggg tgatcatctc
     7261 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     7321 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     7381 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     7441 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     7501 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     7561 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     7621 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     7681 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     7741 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     7801 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     7861 cgcgttttga ccagtggttg catctaatac aagaagtgtt tcttggggtg cttctggtaa
     7921 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     7981 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctgtctttg ctctttccaa
     8041 agcatcgaat acgacagctg ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc
     8101 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     8161 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     8221 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     8281 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     8341 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     8401 tgcttcggta attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     8461 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     8521 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     8581 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     8641 actcccccta ctctaataca aatttttgga tcactttggc tacgccattt tcttcattgc
     8701 tggctgtcac gaaatctgct gcttctttga cgaaaggcac agcattctcc atcgctacgc
     8761 ctagtccagc atattggatc atcggtaaat cattttcctc atcaccgatc gccatgattt
     8821 cttcttgttt caaaccaaga tcttttgcca ataaagaaat accataagct tttgtaacac
     8881 cttttggcat aaattccaaa agcatacttc ttgttttgat gatctcatac atttcatgga
     8941 attctgctgg tatttctttg attttaggat ctaattcttc ttggtcataa gcgattactg
     9001 ccttattaag cacaaattca tctgtaatat cgcttatatc cgctggttgg aattgcagca
     9061 gcttattcaa cacattgtac aaggattgct tccttggggc actaggcagc tgtaaaacca
     9121 cattgtcaga caaaacatct agtggcagat ccaattgctg agccagtttg tataagcgtt
     9181 ggatatctga tacttccatc actttctttt ccatgatttc accagtatca tttttttgta
     9241 ccaaaccgcc gttgaaagtg atgctgtagt caccttcatc taccagtcct aattcttgca
     9301 gataatgcgc catcgcagct aaggggcgtc ctgtacatag aacgatcttg accccttgct
     9361 gttttgcttg tgctaatgct tgtttattct catctgaaat cagcttttgt tcatttaata
     9421 atgtaccgtc caaatcgatt gctactaatt tgatcatttt cgttcctaca ctttcgtttc
     9481 gttgattttc gcctcttcat tgatatcttc catccgtaca gaaacgattt ttgatacacc
     9541 ggattcctgc atcgtcactc cgtacagtac atctgctgcg accatcgttc ctttgcggtg
     9601 tgtgactacg ataaattgtg tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt
     9661 cacgttggct tcatctaacg ctgcttctac ctcatctaag atacaaaacg gaacaggaca
     9721 gacacggata atggaaaaga gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag
     9781 caaacttaga ctttgcagtt ttttgccagg aggttgtact tcaatctcaa tacctgtttt
     9841 cagcaaatca gttggttccg tcagtactaa ttcagcttta ccgccgccaa acatatttgg
     9901 aaagacaatt ttgaattctt gacggatagc ttcaaatact tctttgaatc ttgtccgcac
     9961 ttcatcatcc atctcatcca tcgtttcaaa caattggttt ttcgctgcta acagatcatc
    10021 tcgctgtgtt gctaagaatg tatgtctttc actgacttgt tcgtattgtt cgattgaatt
    10081 taagtttact ggtcccagtt tttcaatctg ttcttttaag gatgaaacct ttgttctact
    10141 gtcttcgata tcggttgttt cttgatagtc ttgggaagct ttctcaaatg tcagctgata
    10201 ttctgactgc agataggtca aatgattatc taaaatcatt tcagctcggt ctttttggac
    10261 ttctaatttg gattgttccg acagtcgtgc tttttgctca cgattacgct ctgccaagac
    10321 tgcttcaagc ttatcgatct ccttttgcct tctatctcgc tgctctttgg cttttgccaa
    10381 ctcggctttt aatgtttctc gctgagcaga taattcgtta atttgttttt ccaaactttc
    10441 ttctgtgact tcatgatcag aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc
    10501 ttcttgacgt tcggttgcct ctgctttttg gactcgggca ccgcggagct ggatctgcag
    10561 atgattaaac tgctctttca agacagcaag atctgcttgt tcttgtgctt tttgactttg
    10621 tacttgtgct cttctggctt ccatctggtc gctttcttga cttaacgatt tgatttcttc
    10681 gtcgattttt tgtcgttgcg attcaagatc tgtttgtttg tctttcaatt cgttcgtttg
    10741 cttttcgtaa tcttcgataa actgctgcag ttcacgtgtc tcaaaagttg agatttgctt
    10801 ttctttttca aaacgttcaa gttcatttat gatgttttgt agctgatttg tcgcttcttg
    10861 ttcttcaaaa cgtaattgtt cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg
    10921 agctgtttct tgttgcagct cctgaacttt tttttcttgt gcttggagtt gtttatctgc
    10981 ttcttcaaat tcactagtca actgtttcaa ttcttgattt tgtacaaaca aactgccttg
    11041 gtttcctcgt ttattcgctc cacctgtcat ggatcctccg gcattcatca catcaccttc
    11101 taaggaaacc actcgatatt gatagcggat cgtttgcgcg attgcattgg cactagttaa
    11161 gtctttggct aaaaggatcg ttcctagcag gttttgaacc acagtttgga tttgatcggg
    11221 ataactgact tgttcactgg caatccctat aaacccttct acagctgctg cttgtgtcaa
    11281 tatatgagcc ggtaattgtc ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc
    11341 tcctctttgc tgtttcaaat acgtgatggc ttgccgagca tctttttcat tttcaacgat
    11401 gacatgctgg gctgccccac ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac
    11461 atcgatcagt tcagccactg ctccgacaat acccgaaagc tgctgtttat gctgcaagac
    11521 caatcgaacc ccttggtaaa aaccaaaata gttttcttgg atttcttgaa ggcttttctt
    11581 acgagcacgt agctgctgga cttgattcat taattgatac attttaggct gttcttttgc
    11641 taatgcttct tggaactttt tcccgttttg ttggatcatt tctaacttct cttttgtttc
    11701 agttaaagat gattttagct gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa
    11761 tcggtctact gatgcttcta cttccgattg cttggctaac gtctgcttgc tttttgctgt
    11821 ttcttgtata tattgacgtt ctagatactt cagttcgttt cctacagctg cttgttcttg
    11881 catcaaatca acatattgat ctcttaattc tgccaataat tctttcgttg atttttggta
    11941 tttttctaat tcttgctgtg tttttatgag atttgcttca gctttttgga tctctgtttc
    12001 tttttctgct gctgctttca tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt
    12061 ttgtgcttct gctaatgaag tttgatattc ttgcgagctt ttttgtgtat gctttgtccg
    12121 ctcctgcaaa acatcttttt gtccttctgt ctgcttcagt ttttccgaaa gatctaataa
    12181 tacttgctga tttttttcga tcagacgatc ggcttgcgca ttttctttac gctgcttcgc
    12241 caaaatggat tcttgttctt ggatcgactc agatagtttg cctaattcta gattgaattt
    12301 cgcaagctgt gcttgtttgt tgtcccaatc tttttttgct gtcttgattt cagcaaccat
    12361 caaagagaca tcggtttgag tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc
    12421 actctgggcg gccaaaggag ttaactgttc ttccaattcg tggatgatgt cttggacgcg
    12481 gctgaggttg tcttctgttt caaaaagctt ctgttctgct ttctttttcc gctgcttgta
    12541 tttcaagaca ccagctgctt cttcaaagat cccacgacgg tcttccggtt tactactgaa
    12601 aatcgcttct acttttcctt gagaaatgat tgagaacgat tcttttccta atccagaatc
    12661 taagaacaat tcttggatat cttttaatct gcagctttgt ttattgatga aaaactcgct
    12721 ttccccagtc cgtcgataac ggcgggttac actgatctca ttgtactcca atggcagata
    12781 atgatcacta ttgtctaaaa tcactgtgac ttctgcaata ttcagctgct tacgggtatc
    12841 ggaaccggca aagatgatat ccggcatttt tccgcctcgc agacttttag cagattgttc
    12901 tcctaatacc cagcggatag cctctgtaat attgcttttt ccgctgccat ttggtccaac
    12961 cactgctgtg acgctatttt caaaatcaat gactgtcttg tctgcaaatg atttaaaacc
    13021 ggctatttca attcgtttca aatacaaagc tttccctcac ttacttttac agtgctgcca
    13081 atgcattttc cgccgcagct tgttctgcta attttttcga tttcccttgt ccagtgccaa
    13141 tcaattgatc atctacataa acttcgatcc aaaatactcg ttcatgagct ggaccttctt
    13201 cattgatcaa tcggtattcg attgatacat cacctgattt ttgcaagact tcttgtagtt
    13261 ttgttttatg atccatctca tgtgaaaaag cacctgcttt gactttagga aaaatcactt
    13321 tttcgatgaa tgcatgggtc gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag
    13381 cttcgaatag gtcgcaaagc aatgctggtc gcgtacgtcc tcctgaattc tcttctccct
    13441 tgcccaacaa aacatattga tcaaagtgac attctttggc aaatttcgac aagctgtctt
    13501 cgcgaacgat cgcagctcgt gtttttgtca attttccttc tggcaaatga ggatataaac
    13561 ggaataaata ctgagaaacc atcagttcta aaacggcatc tcctaaaaat tcaagtcgct
    13621 cattatccga taattgcaga ttgcgatgct cattcacata ggatgaatgg gtaaaagctt
    13681 gttcaagcaa atttagatcg tgaaaaacga tgttatagtt ttcttttagt tcttttgtca
    13741 gctggttgtc cattttccac attcctttta tggatatttt ttcgatactt ccttattata
    13801 ctggtttttt gtcaaatttg aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc
    13861 tagtgattga cgagaagttg tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt
    13921 gtgaaaatgg ctttccatat ttttgcataa atttgactta ataccgagaa actggacttt
    13981 gaacaccgtt tattggagaa gtcatgaaat cagcgccttg acctgaatat tagagaaata
    14041 aacagaaaaa tatcaaaaaa ccgaaaaagg acatcgccct ttttcggtca tttttagtga
    14101 ttatgaacgt gaatctgctg taacagaaac atttgcccat gggtttggta catcataaga
    14161 ccaatcccag tctttgacac ggtcgttgac tggcaatact tcattacgga acaacgttgg
    14221 aatcgcaaat gcttcttcaa atgcatattg ttgccatttt tgatatgctt ctttttggta
    14281 ctcttcatca aatgctttgc ttgaactgat atcgttgatc aattttgtat tttcttctga
    14341 ttcataacga gtgtagttga acgcagcatt tggtccccat agtccacttg gatttggatc
    14401 agttcctgta ttccaagcag cttggtaaac atcaatttca ggatcatcgt tttctaattt
    14461 atcatagaaa gaattgaatt cgatcagacg acctgttgtg tattggacat ccaaaccaat
    14521 ttctttccat tgttggatat agtaatctgc caatggttgc gctgtttccc caccggacat
    14581 cgaagcaaag ttgattgtta gtttatttcc atctttgtct tctcttaaac catcgtcatc
    14641 cacatctttg tagccagcat catctaatag ttttttcgct ttgtctagat cttgtgtgaa
    14701 tcctttcatc tcatcatcat ggtatgactg gaatactgga ggaatcaatg aagtcgcatt
    14761 gcttctcaaa cctgcataga aacgttcacc tactgcatta ttatctaatg catagcccat
    14821 tgcttgacgt aatgatttat cagccatttt tgaatctgga ttgtattcca cactctttgt
    14881 atctgcattc catttaccta acttgaatcc tagataagta taagaagttt gttcacggcc
    14941 taagtttgta tagccagcaa tttctttata ggtatcgtaa gtatctgttg gcatttggaa
    15001 aatcatatca tataatttag agttcaatgc ttcagttgca cttgccatcg gtacagattt
    15061 catcgtgatc tttttcaatt taggcgtacc attccagtag tgctcgtttg gcaagtattc
    15121 tactgattct cctgcaacga tattactcat gtaataaggg ccaaaagtca ctggattttt
    15181 acgtacttga tcgcttgatt ccatatcttt gattgctaca ccttctaacg tatgttttgg
    15241 tgctgcatat gaccagatac caccacccga ttgaagcatg cttgggttta cttctttata
    15301 ttcgatttca actgttttgt cgtctatttt tttgattccg gagattgtgt cggcacttcc
    15361 atcatgatat tcttccatac ctacgatgtt acggaaagtg tcgtcataac ggataccagt
    15421 gtaatcttta ttcccaatga tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc
    15481 accatctgac catttgacgt tgtcttttac tttgattgtc gcttttttcg tatcttcatc
    15541 tagatcaagc gttgcagctc cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag
    15601 cggttcatga gaaggagcca taaaagctgc atcatatgaa tcttggtaaa actcccactg
    15661 gaacaatcct ttaaactgac tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat
    15721 tgcatcatca ttattttttg ttgcaattgg aaaagttaat tcctctttgt tattgccgtt
    15781 gcctccagag ttgtttccag aggtatctgt acctccgccc ccgcatgctg caagaacgac
    15841 agcagacatc agcgctgcaa atccaaaaat cgttttcttc attcctattt cccccttcgc
    15901 ttctatacta gcctaaccgt tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat
    15961 aattgatact caacatcaaa actaaaataa ggattgatgc gggtaaccag atccattgcc
    16021 gattcactaa gacttctccg ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat
    16081 ttgccggcaa gccgaaacct aagaaagtca aagttgtctc gataccgatg tttgccgcaa
    16141 agttcatcgt caaattagta atgattaatg aacttagatt cggcatgatt tcgcggaaca
    16201 tgattttaaa atcattggtt cccattgttt ttgaggcact gacataatct cttcttgctt
    16261 cagataatgt cttgcttcgg aataatcggg ctttagctac ccaataaaat gcactcatga
    16321 tgaatataaa ggaccagaca ttgtatctgg aaataatcga tacgaagacg ataatgatca
    16381 gcatgatcgg tagaatcatg ataaaatcaa cgatccgcat caagatgttg tcaatgatcc
    16441 ctccgtagta gccagagatg atccctaatc caaccccgat gatcgaagta aggatcgtga
    16501 tcgcaaatcc gatcgtaaca gagtttctag cacctatgat caattgtcct aatacatcac
    16561 gtccgccttc atctgctccc agaatgaact tttgtccttg tgtcgaaact gttcctggtg
    16621 cagcgtattt gtcgaagata ctaacataca tcactttgtc ttgatcggtc agcatcgcac
    16681 cgataaagat cactaataat aaaaccacta gcaaacctaa tgaaaaaata gcaattttat
    16741 cttttttaaa ttctcgagca atcattcgta tccccatagg aggaatactt tcttgaactg
    16801 ctgtattgtt tttttcactc atcgatccat tctcctttct gattggtttt attgaatccg
    16861 aatacgcggg tcaacgatac tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc
    16921 gattcctaaa atcaatacta aggatgtgat cacggtatag tcccgttggc cgatggaatc
    16981 aataaatagt tttccgactc ccggataacc aaaaatcttt tcgataacta cagaaccagc
    17041 aatcaacgaa gtgatttcat accccatttg agaagccatc ggcaacgctg catttcggaa
    17101 aatatgtcga ctgaatactt tattcgtcgg tacccctttg gaacgggctg ttctgacaaa
    17161 atcgagcgat ttcgaatcga ttacttcact tcgcagatat tggattgtga cggcagtcgc
    17221 caaaaatgct tgggtaattg atggtaacag aatatatttc aaacggtcta tccattgagt
    17281 gataaatcca ccttgcaaac ctagtgtttg tgatccactt gttgggaacc agcctaaacg
    17341 ataaccaaaa acaaatagca tgatcaagcc gaagataaat aaaggaacag caaaactaaa
    17401 gaagttgtag attacgacaa tcttatccaa gattgaattt tgataccggc cagcaagcat
    17461 ccccagaggc acagcaatca aataagtaag gacaactgtc aagatcgata gatataaagt
    17521 gttgatgatc cgttcgccga tcaagtcagc aacagagcgt ttgtataaga aactttgacc
    17581 aaaatcgcct tgcaaagcat ttccgatcca acggaagtac tggacatacc atgggtcatt
    17641 caagccagct gcttgtcgca tctgttcgat cactgccgga tcctgatttg gattgatcaa
    17701 accggtaaat ggatcccccg gcatcgcttg ggctaataag aagatcagaa cacttaaaat
    17761 aataacttgc gggatcatca ataaaacacg tcgcagaatc gttttccaca ttagcttgca
    17821 cctccatctt tcaacgccac tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga
    17881 ccagtcgcat catagtattc tttttggttt tggatatact cttgttctac tcttcgtcga
    17941 ttttctttgt ggctttccct attttctaca tcgatttgcg gaatagctga aagcagacgt
    18001 ttcgtataga tatgtcgcgg gtcattataa atgtcttctc ttgtaccgat ttctacaaat
    18061 cgacctttgt acataattgc gatattgtcg cacatgtgtt ttactacacc taaatcatgt
    18121 gaaatgaata aatagctgag gccgtattct tgctggattc gtttcatgaa attcaatact
    18181 tgcgcttgaa cagatagatc cagagcagat acaggttcat cggctacgat cagttttgga
    18241 ttggtagcaa cagcacgggc tactcctaga cgttgacgtt gtccgcctga aaattcatga
    18301 ggatatttat atagcgcatc ttccggcatc ccaacgatat ctagcagtcc ttttaccttt
    18361 ttcttttctt cttgatcgct taaccgttca aaatttcgga tgggttcagc aatgatatcc
    18421 aacactcgct ttttaggatt caaactggac attgaatctt ggaagatcat ctgcacatcc
    18481 ttgttataac ccattttttt gcggttgctt cgcttcgtta catcttttcc ttcatacatg
    18541 atttgtccgg ctgttacctt ttctaatccc acaacggctt ttcccgtagt agattttcca
    18601 gagccagact ctcccactaa accatatgtc tttccttttt cgataataaa atccacacca
    18661 tctacagcat atacatgatc cgttactcga ttgaagaatc cactgcgaat agggtaatga
    18721 actttcaggt ctttgattgt gattaattct gtcatctaca cctctccttt ttcgtctcta
    18781 aagtggaaat gcttatagca ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg
    18841 ataggctctt cttcatgatc gcttgctggt atccatggaa tacgtggtgc aaaccgacaa
    18901 cctttacgag gcatgttttt cagagaagga acgacacctt cgatcacatg gagttctgca
    18961 tcatgatttc cttcttgtgg tatagagttc agtaaagagc gtgtataagg atgcttagga
    19021 tgatcaaaca gttcttttac acctgctact tcaacgaact gtccgccata catcactgcc
    19081 actcgatcag ccatttctgc tacgactcct aagtcatgag tgatcaagat gatccctgtt
    19141 tttgtttctt cctgaaggtc tttcagtaaa tcaagaatct gtgcttggat ggttacatct
    19201 agggctgtcg tcggttcatc tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg
    19261 atgatgacac gttgtctcat accgcctgat agttcatggg gatattggcg tccaactctc
    19321 gcaggattag gaatccctac ttgttctagc aattctagtg cacgagcttg acgctgttct
    19381 gcattcatct ttgtgtgata ggaaagtcct tctttgatct ggtcttcgat tctcatgaga
    19441 ggatttaatg cagacaacgg atcttggaaa atcattccga tatcatttcc gcggatttta
    19501 ttatagagcg tttcgttcag gtcgattaaa ttcagatcgt tatataaaat ctcacctgta
    19561 atttttgtgt tattaggatc aagtaatccc ataatcgtag tagccaaggt acttttcccg
    19621 cacccagatt ccccaacgat tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca
    19681 tctactgcat cgtaatagtc atctttgagt cggaatcctg tatgtaggtt ctgaacttct
    19741 aataattggt tatcggacaa tcgaataccc ctctctgctt cctaaatgga agtttttctt
    19801 aataaaaaag gacattacac agaataaatc gttttataac a
//
LOCUS       DARDHD010000036        19451 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000036 DARDHD010000000
VERSION     DARDHD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.036"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..274)
                     /locus_tag="SDA06_001019"
     CDS             complement(<1..274)
                     /locus_tag="SDA06_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228311.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYS"
     gene            428..1123
                     /locus_tag="SDA06_001020"
     CDS             428..1123
                     /locus_tag="SDA06_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4228312.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1565..2362)
                     /locus_tag="SDA06_001021"
     CDS             complement(1565..2362)
                     /locus_tag="SDA06_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG4228313.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2359..3543)
                     /locus_tag="SDA06_001022"
     CDS             complement(2359..3543)
                     /locus_tag="SDA06_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4228314.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3568..4815)
                     /locus_tag="SDA06_001023"
     CDS             complement(3568..4815)
                     /locus_tag="SDA06_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4228315.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5030..6775
                     /gene="dnaX"
                     /locus_tag="SDA06_001024"
     CDS             5030..6775
                     /gene="dnaX"
                     /locus_tag="SDA06_001024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG4228316.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6795..7109
                     /locus_tag="SDA06_001025"
     CDS             6795..7109
                     /locus_tag="SDA06_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG4228317.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7134..7730
                     /gene="recR"
                     /locus_tag="SDA06_001026"
     CDS             7134..7730
                     /gene="recR"
                     /locus_tag="SDA06_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG4228318.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7794..8438
                     /locus_tag="SDA06_001027"
     CDS             7794..8438
                     /locus_tag="SDA06_001027"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG4228319.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8453..8782
                     /locus_tag="SDA06_001028"
     CDS             8453..8782
                     /locus_tag="SDA06_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG4228320.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8796..9734
                     /gene="holB"
                     /locus_tag="SDA06_001029"
     CDS             8796..9734
                     /gene="holB"
                     /locus_tag="SDA06_001029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG4228321.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9770..10594
                     /locus_tag="SDA06_001030"
     CDS             9770..10594
                     /locus_tag="SDA06_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG4228322.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10587..10934
                     /locus_tag="SDA06_001031"
     CDS             10587..10934
                     /locus_tag="SDA06_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG4228323.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            11003..11875
                     /gene="rsmI"
                     /locus_tag="SDA06_001032"
     CDS             11003..11875
                     /gene="rsmI"
                     /locus_tag="SDA06_001032"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG4228324.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11984..13105
                     /gene="dinB"
                     /locus_tag="SDA06_001033"
     CDS             11984..13105
                     /gene="dinB"
                     /locus_tag="SDA06_001033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG4228325.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13159..13761)
                     /gene="nrdG"
                     /locus_tag="SDA06_001034"
     CDS             complement(13159..13761)
                     /gene="nrdG"
                     /locus_tag="SDA06_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG4228326.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14076..16265)
                     /gene="nrdD"
                     /locus_tag="SDA06_001035"
     CDS             complement(14076..16265)
                     /gene="nrdD"
                     /locus_tag="SDA06_001035"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG4228327.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16564..17358
                     /locus_tag="SDA06_001036"
     CDS             16564..17358
                     /locus_tag="SDA06_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228328.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17348..19390
                     /locus_tag="SDA06_001037"
     CDS             17348..19390
                     /locus_tag="SDA06_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228329.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         6104 a         3526 c         4020 g         5801 t
ORIGIN      
        1 ctgaatatgc ttttaatttt tgtatttgaa cttctacatc gtgataattg gatggatagc
       61 gatcaacaag ttggcgaata aataaatctc ttgtagacat aaacagattt aaaaattctt
      121 cgcattcgtt tgttggattc tcatccactg ctttgatcaa ttcgatcgag ttttgatacg
      181 catcatttaa aaagactaaa gaagctttca tatcaaatct tgtttgatat gctgtttcta
      241 atcgattaat gattgtgttc atccagtaat tcatgttttt tccctctatt cttaaaaaat
      301 tttacttctg cagtataaag aataaaaaaa aggaaaaaaa tcgcataagt gatggaaaaa
      361 ggtacttaac aaatgttaat atcattagta aaataccttt ggactttaaa aagttttaaa
      421 gtactgtatg atctttttag atgaaagttt ggatggggga gcatcattgg atagtagagt
      481 cttaaaggaa tatttggata aaaatgattt tccgattata cgcaaaggat atcataaata
      541 tttgacattc gaagggatgg aagattccca tacgtatatc ttgaaagagg gaacggtaaa
      601 agcaagtgtt atttcaaatg atgggagaga atttaacctt cgctatatca aagatcttga
      661 aatcgtctct cttttaaaag acgaatattc acaattcatc gattcgccgt ataatattcg
      721 agttgaatca gaatatgcag agttttatcg aatcaatcgt gtgcgctttt ggcaaaatat
      781 caaccaagac ccaaagctac aacactatgt aaaagagtat taccgctatc gtctgcttta
      841 ttcgatgaaa aaaatgcagc aaatgctatt aaacggaaag ctgggagctg tttgtacgca
      901 actttacgaa ttatatgatt tattcggtgt gcggacagaa gaagggatgc tgatcgattt
      961 ttcgatcacg aatgaagaaa tcgccaaatt ttgtggcatc tcaactgcta gcagtgtaaa
     1021 cagaatttta aaacaactga aaaaagatgg tattatcacc gttaaagacc atcatatcgt
     1081 tattttgaat atggacttgt tagaagacaa tattatcttt tgatgaacaa agaggttgtg
     1141 aaatcagcgt tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
     1201 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt
     1261 gagcctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
     1321 gatgaatatt gtgacttgtg accgaggagt tggtaggaga acaccgttta ccaagaggtt
     1381 gtgaaaaagc tgttctgcat caagtaataa gaagagccaa gtaaaaacag tctcttatgt
     1441 ttttaacttg gctggactta tcaccgtaga tgtaagcttt tgaacgccgt ttatttgcaa
     1501 caaaaggggc tgtgacacga tttttgtcac agcccttttt ttgcgaacat tcatttagca
     1561 atttttagaa acttctgccg ccaccgccaa aagtcccgcc accgctagaa tgggtagtac
     1621 ttccgccacc gccgcttcca ggaggtgggc ttttggggat tcttcttgtc gtgacaaaag
     1681 aatttgtcag acgatcggtt ttttcagtta atttgatact ggatttttct cggaatggat
     1741 atttgtagat gcctgatttc aattgataac gcgaaacagt cacaaaataa aagacaagac
     1801 taaggataag agccaaaaca acagcaatga tgatttctac tgtcgtcaaa accttatagc
     1861 gtgtaatttt gccagtttct tcgtctacac gataatgtcc tcctggaact ccgtcttcca
     1921 catatgttgc tgatttagac aggtaagctt ttgcagcatt gtaataggca gcatttgtca
     1981 tctgatcata aacatcatct aagatcgaat cgattctgct atcagtcaaa taatcgatca
     2041 tattaccaga agtcgaaatg tagatttcac gctgtcccat atctaatagc aatactgagc
     2101 cattttgatc atttccgact gcgtctctta agtaattatc agcaaagtct cttggttctt
     2161 ctgaattaga tgtagtcgtt acgatcaaga ctcgtccttt gattttttca ttcagctttt
     2221 ctgcctgttg ttccaaagat tggatttgat ctgttgtgaa taacccagcc tcatcattta
     2281 ccgtagctgt atctgcatga gtagccaccg gccagccaac acttactagc aaaagaaaga
     2341 ataaaaaaaa ctttttcatc atatcaagta acctccgatc agaaagagga tcgctagccc
     2401 agcgaaaatc ccaagagcgg tcagtcccaa ttttttctgg ctgatcggca ggacaccgct
     2461 gacctttcct gtttgtccat tcattgcgta gtagtagact tttttacttg agtcattgct
     2521 tcgataagta acaagccata ccggcagtaa tacgtaatta tttacttcgt ttgtgattga
     2581 agcagaagtt cgcacaccgg tcaatgttgt atatccattt gcggtatctt ttaacagctt
     2641 ctctgaatag tcttttaatt cagattgaat ctgtgatttg attgcctcat attcgatatc
     2701 tcttttttct gcttggaacc cggctaaata ctggctttta aaatcaattg ctttatctaa
     2761 cgggaaaggt tgcacgcctt cgaccatctt ttgttgcgta ttttttgata aggcattttt
     2821 tactagttct ttaaaagaaa gcttgcctgc tcttgaaaca gcaaattgct tcgtttctgt
     2881 gtattcgatg tcacctatcc gccatatgcg gataactgta ccattcgcct gcatggcacc
     2941 atctacttca gcatctgtag cccaataagg gaaatacaca cctgtcaact tatcgatttg
     3001 atccttattg aaaaaagctt tgggaacaaa ccattttttc ttggtccaag cgagaaatct
     3061 gttgattgct tcttctttct cgacagcaaa aggaagtact ttatttggca agaattctcc
     3121 gcttaaacgt cccgatagta cgacgggatt atggcagtag taacaataag tcgcagcagt
     3181 tgtcgcatca gtcacgatct ctgcaccaca gttgggacaa agaaaaagat ctatcgctgc
     3241 ggtattatct gcagattcgt ccgctgtatc tgaaaaaccg cctgcctcgt caaccgcttt
     3301 gcgctcggct tcattcatat cggcaagctg ttcttgggct gtaaaagtaa aatcttcggt
     3361 aggtgaaggt tcttctttgc tttcttcgcc agccatacgc gcttcttttt gcttttgctc
     3421 atattggctg acttcttctt ctgtatagat attcaggcag tattcgcaat gaaatttttg
     3481 gtctttggga tcaaaagtca acggaccgcc gcaattagga catttatgtg taagaacatc
     3541 catcagctgc acacctttct aaataaatta attaacgggg attcctttaa acggtttacc
     3601 acagtttgga cagaatttag ggatgccgtt ggaaagatcc acgatctcat tgcattcact
     3661 gcatcgcatt tgtagcttag gctgaacgtt ttctacaggt ttggcagctc cgcagttgga
     3721 acagaatttt ccggaatttt ctgtcccgca aactggacat gtccaagtat ccgcatttcc
     3781 ttgtgcagtt tgttgatttt gtttttctgc ttgttgtttg atttgttctt ggttattttg
     3841 agctgcttga gaaagataag aaccatttgc attcattccc atacctacac ccataaagcc
     3901 ggtcattgct cctgcttcat tttttccggc tgcttccata ccacgtgcaa tggaaccttg
     3961 cacgtatcct tcacgtacgc taggatcacc tagcatcgcc ccttcgttac gcatattgat
     4021 cagtttcaca gaatcatcgg tgtaagaaat gctggctaca gccacagaaa caatctccat
     4081 cccacgtaat tcacgccaag aatcatccaa tgcagtatcc atgtatttgc ttaattccaa
     4141 actcttagat ggtacataag aaatccgttg accatctgca gacatctgat tgattgctga
     4201 ttgtagcgct gttaggaatt cagctaaata ctgctcattg atatcattga tttccacttg
     4261 tgttttgttt ttagggattg cgtttgtata aaataaaatc gggtcaacga catgaataga
     4321 ataagtgcca tgtgcccgta agaataattc tgcgttatag aaattgtcaa agtagttcaa
     4381 cggcgcgctt gtaccgaatt tgattccctt tatttcttgg agattgatat aaaaaacttg
     4441 ttgtttctgc ggtgtgacgc cgccaaattt gaatcggtca aaagtttcag caattgcttc
     4501 tttcagtgtt ccattgaaca tggaaggagc tgcgtcattt ttaatggtgt aataaccttc
     4561 ctcagcggta taatcgatga tttttccacc atcgaccaaa agcatcatca tgtttggata
     4621 gacatggacg actgttccgt ctgttaaaac atcttctgtt cctttacgat tggagccacg
     4681 tttgtcatct ttgcggactt tgacaccttt tgtcatgacc gttgtatcac tcatattgtc
     4741 tggttcgatg acttctagcc attgatctgc cagaccgccg ccaaccatac ttgttgctgc
     4801 tttgataaga cccataaaaa attcctcctc tgattcagct agtgccaaac aattattgtt
     4861 tatttattat accataggaa gatgtgaaac ttctcggact agagacggaa ttgaaatgga
     4921 aaattgcgta ttgtttaaat acctgaaaaa gccgagaagg atagtagttt ttttacctaa
     4981 tatcacgtat aataagagat agtgaatttt ttaaaaaagg gggatcctta tggcatatca
     5041 agcactttat cgagtctggc gttcgcagcg tttcgaagac atcgttggac aaaaagctgt
     5101 gacacagaca ctaaaaaatg ctattgtttc ccacaaaacc tcacatgcct acttgtttac
     5161 aggacccaga ggaaccggaa agacgagtgc agcaaagatt ttcgcaaaag caatcaactg
     5221 tccgaacagt aaagacggtg agccatgcaa cgaatgcgaa atgtgtcgat caattactgc
     5281 tggtacgcaa gaagatgtga tcgaaatcga tgctgccagc aataacggag tcgaagaaat
     5341 ccgatttatc cgtgatcggg caaattatgc gcctacaaag gcagcgtaca aagtgtacat
     5401 tattgatgag gtacatatgc tttcaacagg agctttcaat gccttgttga aaacattgga
     5461 agagccaaaa gaaagtgtca tattcatctt agcaacgacc gaacctcaca aaataccagc
     5521 tacgatcatt tcacggactc agcgatttga ttttaaacgg atcaatacgg cagatattgt
     5581 cgaacatctt gaatttatct tgaaagaatc gactatcagc tatgaaccag ctgcattgaa
     5641 tgtgattgcc cgttctgcag aaggcgggat gcgggatgca ttgagtattc ttgatcaggc
     5701 aatctcgttt agtgaaggaa caattacttt agatgatgcc atgcaagtga caggtagctt
     5761 aactgacgaa atgatggaca gttacttgag tgcttgcgtg aaaaaagaag taccagaagc
     5821 attggaaaca ttgaaccaaa ttctcgcagc cggcaaagaa tcacgtcgct ttctagagga
     5881 tatgttacaa tactgccgtg acttgctgat gtatcagcaa gcacctaacc tagttgagga
     5941 acaatcgagt acgatcagaa gtacatttac agaattggca caaactgttt cagcggatca
     6001 gctgttttca atgataaaga ttttgagcga tactcaaaat gaaattcgtt ttaccaatag
     6061 tgccgctatt tatttggaag tcgctacagt caaacttgca cagctaccgt ctgcttctgt
     6121 tccttcagca gatgtagcat ttgcagatga tcagatagta aaacagctac aacaagagat
     6181 taaggaacta agaaacgaaa tcaaggaact acgcagtaat acaggaattg agaatacaaa
     6241 agtacagccg gtacagcaaa caaatcataa aaaacagaca agtacctatc gtgtaccgac
     6301 ggaacgagta tataaagttc tgcgggaagc gacaagacag catctgaacc aagtaaaaga
     6361 agtatgggaa gacttgctga tgagtctatc tgtcactcag cgagcaatgc ttaaagcaag
     6421 cgaaccagtt gcagctggac ctacaggact agtgattgcg tttgattatg agattgtttg
     6481 tcaaagagca gcaaatgacg aagaattgca gctttcggta cacaacaact taagccgaat
     6541 gatcaaagat tatgcgcctg atcctgttta tattacaaga gaaagctggc cagaacttcg
     6601 aagaaattat ttgacgcagg cacaagaaga gggaacagaa aactcttttg cttctgaaga
     6661 ctcagaagga gaaatcgagt tgcttcctca agaacaagaa gtaccagaag aaacacttgt
     6721 agtaaaagca gaggaactgt ttggctccga agctgttaca gtggtagatg attaataaaa
     6781 aggagtagat aattatgatg cgtggaatgg gcaatatgca aggcatgatg aaacaagtac
     6841 aaaaaatgca aaaagaaatg gcagaagcac aagagctgct aaataaaaca gagtttacag
     6901 gtaaagccac caatgaattg gtcaaagcgg tttttacagg cgatcgtcgc atgaaagata
     6961 tccaaatcaa tgaagaagtc gttgatcctg atgatatcga gatgcttcag gatcttgtcg
     7021 taatggcagt caatgatgcg atcgcacaaa ttgatgtgga aactgagaaa aaattaggga
     7081 aatacgcaaa aggtttgcca ggattttagt tttaaactga aggagtaatg aatatgcaat
     7141 atccagaacc aattgcgaaa ctgatcgaca gctacatgaa acttccagga atcggtcaga
     7201 aaacagcgac aagactcgcc ttttatacga tcgatatgaa agatgaagta gtaaatgaat
     7261 ttgctaaatc tttgttaagt gtaaaacgcg atttgcattt ttgtagtatc tgcggaaaca
     7321 tcacagaaga agatccctgc gaaatatgta gaaatccttc acgtgatcga agcgttatcc
     7381 tagtggtcga agagccgaaa gatgtaatgg caatggaaaa aatgcgagaa tatcacggac
     7441 tttatcatgt tctccatggt gtgttatcac cgatggaagg gacaggtcca gaagacatca
     7501 atatttcttc tctgttgcag cggctgcatg atgcagatgt aaaagaagtg attatcgcaa
     7561 caaatgcaac aacagaggga gaagcaacag ctatgtatct ctcacgtctg atcaaacccg
     7621 caggtatcaa agtgactcgt ctagcacatg gtctgtctgt aggctctgat atcgagtatg
     7681 cagatgaagt tactttattg aaagctgtcg aaggaagacg tgagttataa atttctaatt
     7741 aagtgttttt tcagatacaa tataaaagca gcacaagaga tgggggcaac aatgtgaacg
     7801 gactatttat tacaatcgag gggcctgatg gtgcaggaaa aaccagcatc ttgaatgaac
     7861 tttaccctct tttgaaagaa gtagcaaaaa cagaaatcat tcaaacaaga gaaccaggag
     7921 gcattccgat tgctgaagag atccgagcag tgatccttga tccaaaaaat gatcggatgg
     7981 atgaacgtac ggaagcactg ctttatgcag cagcaagaag acaacatcta gtcgaaaagg
     8041 ttctgcctgc tctggcaaaa gggaaaatcg ttttatgcga tagatttgtc gacagttctc
     8101 ttgcttatca aggtgcaggc cgacgcatcg gcgttaccga gattgctcgg ttgaatgagt
     8161 ttgctacaga aggtactacg ccggatttta ccctttatct tgacgtagat tcagacaccg
     8221 gattaagaag aatcaagaaa aacagacaaa atcaaatcga ccgtttagat tcggaaggtt
     8281 tggaattcca ccagcgtgta cgtcatgctt acttaaagct ggcagaagag aatccggaaa
     8341 gaatccacaa agttgatgca agaaaaagct ttgaagaagt gctgcaaaca agttatcata
     8401 caattattga acagtatccg caattttttg aaaactagga aggtgaacgt ttatgaaaat
     8461 tattttagcg attatccaag acaaagacag caaccgttta tcaaatgaat tgattgatgc
     8521 aaatatccgt gccacaaaac tatcatcgac aggcggcttc cttaaggctg gaaacagtac
     8581 gtttatcgtg ggtattgaag atgaccgtgt agaagaagca ttggaaatca tcaaaaaaac
     8641 gtgtgaatca agaaagcaat ttgtatctac acctgttaca ctggatattt cgatggatgg
     8701 gggcgtccct tatccagtag aagtagaagt aggcggtgcc acagtcttcg tcttacctgt
     8761 tgaaggattc catcaatttt aggaggagct gagctatgaa tcaagaaata aacttggctc
     8821 aaatgcaacc gatcgtgtat cagcagcttc aacggagttt tgagcatggg cgtatcgctc
     8881 atgcttatct ttttgaaggg gagaaaggaa caggaaaaca tgaagtaggt atatggcttg
     8941 cacagcacct gttctgtacc caaatgaaag acaatctgcc ttgtggaaaa tgcaacaatt
     9001 gtcagcggat cgaaaagaaa gaacatccag atgttttggt tattgaaccg gaaggacaaa
     9061 ccatcaaagt cgatcagatc agacgtttgc agacggaatt cagtcgcagc ggttacgaat
     9121 caagaaaaaa agtttttttg atcaaagaag ctgaaaaaat gaatagcagt gcggcaaaca
     9181 gtttgttgaa attcttggaa gagccccctg gagatttttt agcggtgtta gaaacagatg
     9241 cagtaggaag aattctccct actattcaat ctcgttgtca agttctgcat tttcaagaac
     9301 tttcgaaaga agcactgatc catcgattgc aagaagagca gattccattg gaaaaagcaa
     9361 aacttttggc tttcttaaca aacagtttag gaaaagcagt tgaaatctct caagatgaat
     9421 ggtttaatga tgctaaggac ttgatttatc aatggttcgt ttacttgcaa aagcaagata
     9481 ctcaagcctt tatctatgtc caaaaaaaat tggtaaaatc atttaaagaa aaaacacaac
     9541 agtttacagg attatctgtc ttgatgtttt attatcagga agcacagcgt caagcagtag
     9601 caaacggcga atatggcaaa gtgaaacaga taaaccagat aattgaacgg atattgatcg
     9661 cagagcagaa actacgcagc aatgtcagtt ttcaggctgt tgcggaacag ttcgttttgc
     9721 atacgatttt ttagcttgcc gcaaaggatg atgatcaagg aggatggaaa tggtagaagt
     9781 agtaggtgtc cgatatagag atgcaggtca tatctattat tttgcaccag gagactctga
     9841 atatgtttat agtgacaagg tattagtcga atctcagcaa tccttacata ttgctactgt
     9901 tgtccttcct aaaaaagaga ttgatcccaa tgaccttcct gaagctatcc gtccgatctt
     9961 gcataaagcg tcacaagctg aattagaaaa agacaaaaaa aacaaaaaag atgcttatga
    10021 tgcattgaaa atcgcaaaag aaaaagtgca gttgcgtgag ttggaaatga aaatggtcaa
    10081 agccgaatac acatttgatc gaaataaatt gattttttct ttcacagcgg acgggcgaat
    10141 tgactttaga gaattggtaa aagatctggc cgctgtattt cgtactcgta ttgagcttcg
    10201 acaaattggt gtccgcgatg aagcaaagat aataggcgga attggtccat gtgggcgtcc
    10261 attatgctgt tcttcttttt taggcgattt cgtgcctgta tcaatcaaaa tggcaaaaga
    10321 tcaaagttta tctttgaatc caacaaaaat ttccggttta tgcggacgct tgatgtgctg
    10381 tctgcaatat gagagcgaag aatacgagac agcgaaaaaa gaactccctg actttggaaa
    10441 agtaattgct acacctgatg gtaaagggaa agtcgtaggg ttgaatctgc ttagccgtat
    10501 cgttagtatt cggctgactg gacgggaaaa tcctgtcgaa tatgaatggg atgaattgaa
    10561 agaatttatc caagtaggtg agatgaatgg ataagagatc attatacgac ggattgaatc
    10621 aattagaatc agagttgaga aatacattaa cagaacttgt tgaaatgaag catgctttgc
    10681 atgagatcgt agaaaaaaat acaactttgg agatggaaaa tcaacgtctg cgggaacacc
    10741 tgcgggaatt gaaccaagtt gcacaagctc cagctgaaaa tgtgaagcag gaactttcta
    10801 agtctcgtat gaatcttgaa aaaatttatg aagaaggatt ccatgtctgc tacgatttat
    10861 atggatcaag aagggacaat gacgagcctt gtgcgttttg tctagaagtg atctatcgcg
    10921 aaagcggtcg atgaccaata acaagaggag actggagcgg agaaatactg ctccagtctt
    10981 tttattaagg agattgtgac agatgtataa tcaaaaaagt ttcaagggaa atccggtata
    11041 tggggattta tatcttgtac cgacgccaat tggaaattta gaagatatga gctacagaag
    11101 tgttcgtatg ctgcaagaat ccgatcttat cgcaagtgag gatacgcgta atacacaaaa
    11161 gttattgaat cattttgaaa tccatacacc ccaaaaaagt ttacatgaac ataattataa
    11221 agaacgtgtc cctcaattga ttgctttgtt aaaagaagga aaaacgattg ctcaagtcag
    11281 tgacgcagga atgccttcca tcagcgatcc tggacatgag ctagttttag cctgcataaa
    11341 agaagggatt tctgtgatct ctatcccagg acctacagct gggatgacgg ctttgattgc
    11401 ttcagggcta gtgcctcaac catttttatt ttatggcttc cttccacgta agaaaaaaga
    11461 acagaaagaa acattgaaaa agctaaaaga acagacagtt acgctgattt tttatgagtc
    11521 accttatcgg atcactgcta cattgacgaa ttttttagaa atttttggaa atcgccaagt
    11581 cgttttgtgt cgggaattga caaaggtcca tgaagaatac cttagaggaa gtacggaaga
    11641 gctactcgct tatatcgaag aacatccagt aaagggtgaa tgttgtctgc ttgtagaagg
    11701 gaatttgttc gaaatgccag tgacggaagt tgatgaagag ttgggaacgt taaaggaaca
    11761 agtagaagca aagatcgctt caggcgaaaa accaaatgct gctatcaaag cagtagcgct
    11821 gaaaaatggg ctgaaaaaac aagaagtcta ccgtcagtac catgaattag actgagatag
    11881 tataagttgt ctataatgaa agactaatga ggaaatcagc tactaacttt tcaaatctct
    11941 tgaacgcgaa cgaatgttct tgtataatga ataagaggtg aggatgatgg agctgcaatt
    12001 tcctttacat aatgatacat caagaaaaat cattcatatt gatatggatg cttttttcgc
    12061 ttcggttgaa gaacgagatc atcctgagtt tcgcggacat ccattagtaa ttgccagaca
    12121 ccccagtgac actggtgggc gtggagtagt gactacagca aattatgaag caagaaaatt
    12181 cggaattcat tcagcaatga gtgcacaaaa agcgtatgag ctttgtccgc aagccatctt
    12241 taagcctggc gaccatcata aatacagcga tatttccaaa caagtacggg aagtattcta
    12301 tcgttataca gatattgtgg aaccagttag tatcgatgaa gcgtatcttg atgtgaccga
    12361 aaataaaatc aattgcaagt cagccatcaa aatcgcccga atgatccaag cagatatttg
    12421 ggagagtgtc cagctaacat gttctgcggg agtaagttac aataagtttt tggccaaatt
    12481 agcatcagac tatcaaaaac ctagaggaat cacggtagtt gcccctcaag acgctgtgcc
    12541 atttttaaaa gctttgccaa ttgaaaaatt tcatggggta gggaagaaaa ctgtacctag
    12601 aatgcatgag ctagggattt atacaggaaa agacctatat gaatgcacag aaatgatgtt
    12661 gattcgaaat tttggaaaaa tgggctattc tctttatcgt aaagtcagag gaatccatga
    12721 ttctccagta aacgttacaa gagagcgaaa atctgtggga aaagagcata cttatgggca
    12781 gccgttgcaa acggaagaag cagtgctgac tcagttgcgc caactagctg aaaaagtaga
    12841 agaagcactg agaagagtcc aaaaacatgg gaaaacagta gttttgaagg tcaggtacac
    12901 ggactattct actgtgacca aaagagtgac attgccagaa tatatctata aaaaagaagc
    12961 actcttttat caagcaagcc ttatatggga ggaaatcttg ggagtagaaa aaggaatccg
    13021 attgttaggg attactttga cgaatctgga cccgatgact tatgaaaaca tcgtccttcc
    13081 attatgggaa aatcaagaaa tataaaaaca gctttgagag ttgaaaccgg aaatccgttt
    13141 tctctcccaa agctgttttt atatcaagtc ttgctttttg atttgttcat aggtttctgt
    13201 tgcatccgta catttttccc aaatcacaac ttgattggat tctaaagatt tttggacgtc
    13261 aatgatccgc tggttgctac ttccgcgaaa ttgtagtttt aggtcacgtt tgcttaattc
    13321 aaagcgtccg tcaacaagga tatcgatttg cgaaaggagt tctaatttat cttctgaatc
    13381 ttgtagcaat tcatcaaagg catagcctgt ccatgaccag atatcttttg tcgtaccgaa
    13441 ctcttttcgg atacgtcgta cgacttgcag acaaacatct gtattcaaaa aaggttcgcc
    13501 accaagtaaa gttaatcctt gcacgtaatc gttgcgcaag tcagcgatga tttgttcttc
    13561 taattcaggt gtgaatggtt tgccatatcg gaaattttgt gcagctttgt tgaagcagcc
    13621 ttcgcaggca aataagcagc cactaacgta aagactgttg cggacacctt caccatcgac
    13681 aaaattaaac ggtttgtagt cagcaatata gttttggctg tagtcttttg ctaaccattc
    13741 ttttggtttt ggatttctca taaaaaaact tctttcattt aaggttcaaa gatatagcta
    13801 ttgaagataa aatctactcg accacataaa taagaacaaa ctgacctaaa tccattgttc
    13861 aagaaagctt gttcttattt tgatagaggg cgttcaaaga tacaaagcca tcgaaagagg
    13921 gtggtgaaaa aagacttgtc atagcctctt attctgaata aacggtgttc aaaagcttgc
    13981 atctgcagta ataagcccaa ctaaacgaaa aatatgagga actatttttg tttagctatt
    14041 cttattactt gatgcaggac agctttttca caacctcatt tcatgtgttt cacacgtgag
    14101 gaaatttctt tatggcggcc atgtaccatc ggacgagctt gcggatttcc taaatagcca
    14161 caagtacgtt tgactacatc acatgttttt ggatcatgat ttccacattc tggacattcg
    14221 aaccctcgtt ctgtcggttt gaaatcacct tcgaaaccac attgatagca atgatcgatt
    14281 ggcgtattcg taccaagata cccgattttg tcatatgcaa aatcccatac agcttctaaa
    14341 gctttcggat tttgttggag catagggtat tcacagtagt ggatgaatcc acctgaacaa
    14401 tattttggat aatccttctc aaattccaat ttttcaaatg gcgttgggtt tttacgcaca
    14461 tcatagtgga aactgtttgt ataatattct ttatctgtga tattttcgat gatgccgaat
    14521 ttttctgtat ccaaacggca gaaacggtca gttagacttt cacttggagt tgaatagaca
    14581 ctgaaatgat aaccatattc atccccccat gcatctgcat tagcttttaa ttctttgacg
    14641 atatcaagtg tgaaagcttt tgcttctggg tttgtttccc attcgccacc gaagaaagct
    14701 gaagcaactt catacaatcc aatgtagcct aacgatacag tagctcgacg gtttttgaat
    14761 aattcattga cagaatcggt acgtgaaaga cgtttcccga aagaaccata catatataaa
    14821 attggcgcat ttgctggaat ggcttcttta caacgttcta cacgatagag aagcgcatct
    14881 ttcatgattt ctaagcggtc agacagtaac tgccagaatt tattctgatc tccttttgat
    14941 tcgatcgcga ttcttggcaa attcaatgta acaacaccta aattcattcg tccgacattt
    15001 acttcttgac cattttcgtc tttccaccct tgcaagaatg aacgacagcc cataggtact
    15061 ttgaaacttc cagttaattc aacgatttta tcgtaattca gaatatcagg atacattctt
    15121 ttcgttgcac attccaatgc tagttgtttg atgtcgtaat taggatcttc agggtttaag
    15181 ttcacacctt ttttcaaagt gaagatcagt ttagggaaga tagctgtccg cttttcactc
    15241 cctaatccat tgattcgaat ctgtaaaatt gctttttgaa tttctctttc aaaccagttt
    15301 tctcctagtc caaatcctaa tgaagtgaaa ggagtttgtc catttgaagt aaataaggta
    15361 ttgatttcat attccaaact ttgcatagca tcgaagatat cttttttggt ttttgctttt
    15421 gcatattctt tttggcgttc tggcgaatcg atccattctt ccgcgtcttt tagatgtttt
    15481 ttatagttca attctgcaaa aggagcaagc aattcatctg ttcggtcggc agaacagcca
    15541 ccatattgac ttgacgctac attggcaata atctgtgaga tttgtgctgt ggcggtctgg
    15601 atagattttg gcgattcgac ttcggcattg ccgattttga acccattgtt cagcatgcct
    15661 ttgaaatcaa tcaagcagca attcgtcatc ggtgtataag gatgataatc caaatcatgg
    15721 taatggatat cgcctttttg atgcgcattt gctatatgtg gtggcaacat tttcagtccg
    15781 attgattttc caacgatccc ggctgtcaga tctcgctgtg tattgaaaac attactgtct
    15841 ttatttgcgt tttcgttcac gacagtttgg tccttgttga ttagcttttc aatcgaaaag
    15901 ttgatatctg ttgctttact gcgagcaaaa tctcgtttgg tacgataatt gatgtactct
    15961 tctgctaatt catattcgtt tttttctagt aatatatgtt ccacgatgtt ttgaatctca
    16021 tagattttga cattttcagc aaaccggctt ttgatttctt ggacaaccaa gtctgcaatc
    16081 atttgaattt tttcataagt aagtggtgat aatgagccgt gaattttctt ttctgctttg
    16141 atcagtgctt cattgatctt ttgttcatcg aaatcaacat ggcggccatc gcgtttgatg
    16201 actttcatct gggatgtctc tggctgctga gtaacgacag ttttttcttc ataatttact
    16261 ttcattatct tcaactcctg ctaaattgtt cttgtctatc ataaaacaaa atcggggaag
    16321 gagcaactaa atatcggcta tatatagtat tcaatgtgct gtataattgg atttctatac
    16381 tatatgtagt gtatatttta ttgtgaaaaa aggtgaaaac ttgtagctgg tggatttgtt
    16441 ttttgcatat ttaattttgc tgaataaaat aattgaataa aattttttat tcagtccaag
    16501 taaaagtgac aaaaacataa gagcaaatta ttcgtagata tgctactatg caaataaata
    16561 gaaatgaaaa atgaaagagg aggagaagaa ttggaaaaga tgatcgaagt gaaacatcta
    16621 agtaaaagct acggcagcaa actttctccc gtcaaagcct tagataatat ctcattcaca
    16681 gtagaagaag gcgagtttgt tgggattatg ggcccaagtg gtgcaggaaa gacaacgctg
    16741 atgaatatct tgtccacgat caatttgcct acgatgggca gtgtctcgat ccaaggaaaa
    16801 gaaatcacaa aaatgaaaaa cagtgagctc agtgactttc gtcgaaaaaa actaggattc
    16861 atttttcaag agttcaattt gatcgatacc ctaaatgcaa aagacaatat cctattgccc
    16921 ctagcagttg aacggatgac taaagaggaa atggaaaaga gagtaagaca tgtagctcaa
    16981 ttgttgaata tagaagaact gctcaaacgg tatcccgatg aactttctgt cggacagaga
    17041 caacgaattg cagctgctag agcacttgtt gttcagccac aagtgatttt tgcagatgaa
    17101 ccaactggct ctttggactc gaagtcggct acagaattgt tgaactactt gaagaccatg
    17161 aatcaaaaag aaaaagcaac gatactgtta gtcacccatg atccatatac agcaagctac
    17221 tgcgatagaa ttttatttat taaagatggt gtgatttttt ctgaagttgt cagaagaggg
    17281 acaagaagag agttctttga gaaagtaata gacatgcagg cgacgattgg cggaggagga
    17341 aaaatgaatg ctgtataaac tagccgttaa aagttttttt cggcagtcac gaggctatct
    17401 tgtttatttt ttcagtttga ctttatccgt aatgatctat tattccttca gcgcaatgac
    17461 ctacgatcaa tctttagtca gaagagcaag tcaagatact tcgatagatg gtggattgag
    17521 ttttggtgga tttatcatta ctgttgtttt gctttttttc gtattcagtg ccaaccgatt
    17581 tttcttgaat agaaggcaaa aggaaatagg catctatcag ctttttggta tgaataaact
    17641 gcagatatca ggtatatatg tgttagaaat catgataatt ggattgttcg cctgtatttc
    17701 tgggatatta ctcggtatta ttttttccaa actcttttca atgattttgg ttcgaatgat
    17761 ggatatggat cttactagtc cattttttat ctccattcca tcagtagtag atactttatt
    17821 tgcttttttc atcatccttt tagcagtatc tgtatattca atatggaaaa tatggcgcta
    17881 tccaatgatt cgaagttttg gagaaaaaga acaagcagat agttcaacaa tgcgcttcag
    17941 gacaaggcac cgattactcg cagtcatcgg gttactactt ttaacgagcg gatattttgg
    18001 agctagtcat tttagggaaa tcacttcatg gatgatcagt tatcattatc ttgatacatt
    18061 agttttcttt tttccttttt taattttttt cgtttgtgtg gtcggtactt atttgttttt
    18121 ctgttataca tttcggttat ttttgatcat attgagtaaa tggaagattt attactatgg
    18181 tatcaatttt ttgataatgg gtaacacgca agttcatttg ctcaaaagtt ggcgtacaaa
    18241 ttctctgatt acagtcattc tcggaatttc tttaatgaca attggcggca cggctagtat
    18301 ctctactatt ttatcctacc aggagaaagt ttcctccccg atggactatc agtttgatgt
    18361 tgagacagag aaaaaaatca agccgattct agcagaggaa gggcagaaga taactaaaga
    18421 aatcaccttg cattataaag tcattgggag tatctatagt caaaatctcg aagaacctat
    18481 gtttcaaact gctaatctga tcacggaaac acaatatcaa gccttcagaa aagttaatcc
    18541 agatttgccg aacatttctt tgaaatcacc tggtcatact gttttgcttg atcaaatgca
    18601 aactcttttt cggaatattt cattatatgg atctagtatt ggactgccag ataatcaaga
    18661 attgaagatt caacaaatgc ttccaagtgt attaggtgac gaaacaatgg tatatctaag
    18721 tcctgtactg attgtagatg ataccgtttt tgaaaaagca aaaggtgtgg attatcaagt
    18781 agtcaatgta gatgtatcag gtggaaataa tgaaaaaata gatgaacgac tgagcaatga
    18841 cctgtcgata aagtggctca atgctgttta ttattcttat gatatcgtaa atggaagtct
    18901 acaaggagaa attagcacaa agaaacttcc ggatgaccaa atgaaggaag cacaatttag
    18961 ccaagaaagc tctcgtttga atttctcttc cagctattcg aaattgcgtg cagttaatcg
    19021 gcgaatcggt atcaatattt ttgtcacact atttgtcggg atgatcgtga ttattgctac
    19081 tggtagtatt ttgacagtcc ggcaattagc agaagcagag gaagaaaaag aaaattatca
    19141 cctattgact aagctgggga ttccacaaag aaggatcaga cggatgatct tcgagcaaaa
    19201 cgcacttacc ttctttccac ccatgatatt agggatcact cacgcagtat ttgcaatcaa
    19261 tgtcttcact cagtatataa aaactgccga ttactggttg gcttatcttg tgagtggctt
    19321 attgatcctt atctatcttt tgctttatgt cattacgagt cagttatatt gtcggattat
    19381 cgaggaatga tatacggaat aagacaagat aataaataga actaaatttt gaataccatt
    19441 tatttggaat a
//
LOCUS       DARDHD010000037        19083 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000037 DARDHD010000000
VERSION     DARDHD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.037"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..530
                     /locus_tag="SDA06_001038"
     CDS             <1..530
                     /locus_tag="SDA06_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4228330.1"
                     /translation="TNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRMSDIIGLKVG
                     DIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRHITRDRV
                     YQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRYIGIT
                     EDEIGASLRGFKLGV"
     gene            complement(703..1176)
                     /locus_tag="SDA06_001039"
     CDS             complement(703..1176)
                     /locus_tag="SDA06_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4228331.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1188..1724
                     /locus_tag="SDA06_001040"
     CDS             1188..1724
                     /locus_tag="SDA06_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228332.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1764..2135
                     /locus_tag="SDA06_001041"
     CDS             1764..2135
                     /locus_tag="SDA06_001041"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228333.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2419..3690
                     /locus_tag="SDA06_001042"
     CDS             2419..3690
                     /locus_tag="SDA06_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4228334.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3935..4960
                     /locus_tag="SDA06_001043"
     CDS             3935..4960
                     /locus_tag="SDA06_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4228335.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5254..6363
                     /locus_tag="SDA06_001044"
     CDS             5254..6363
                     /locus_tag="SDA06_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4228336.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6446..7342
                     /locus_tag="SDA06_001045"
                     /pseudo
     CDS             6446..7342
                     /locus_tag="SDA06_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7358..8182
                     /locus_tag="SDA06_001046"
     CDS             7358..8182
                     /locus_tag="SDA06_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228337.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8214..9524
                     /locus_tag="SDA06_001047"
     CDS             8214..9524
                     /locus_tag="SDA06_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG4228338.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9672..10931
                     /locus_tag="SDA06_001048"
     CDS             9672..10931
                     /locus_tag="SDA06_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4228339.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10941..11813
                     /locus_tag="SDA06_001049"
     CDS             10941..11813
                     /locus_tag="SDA06_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228340.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11825..12655
                     /locus_tag="SDA06_001050"
     CDS             11825..12655
                     /locus_tag="SDA06_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228341.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12695..14878
                     /locus_tag="SDA06_001051"
     CDS             12695..14878
                     /locus_tag="SDA06_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4228342.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14939..16159
                     /locus_tag="SDA06_001052"
     CDS             14939..16159
                     /locus_tag="SDA06_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG4228343.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16156..17616
                     /gene="gtfA"
                     /locus_tag="SDA06_001053"
     CDS             16156..17616
                     /gene="gtfA"
                     /locus_tag="SDA06_001053"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG4228344.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17693..18799
                     /locus_tag="SDA06_001054"
                     /pseudo
     CDS             17693..18799
                     /locus_tag="SDA06_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6476 a         2908 c         3722 g         5977 t
ORIGIN      
        1 ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt aatccggaaa
       61 gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca gatattatag
      121 gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa cataaaacag
      181 gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta tatactgaag
      241 gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat ataacaaggg
      301 atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat gatataggaa
      361 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
      421 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
      481 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
      541 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
      601 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
      661 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
      721 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
      781 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
      841 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
      901 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
      961 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     1021 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     1081 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     1141 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     1201 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     1261 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     1321 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     1381 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     1441 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     1501 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     1561 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     1621 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     1681 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     1741 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     1801 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     1861 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     1921 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     1981 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     2041 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     2101 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     2161 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     2221 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     2281 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     2341 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     2401 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     2461 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     2521 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     2581 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     2641 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     2701 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     2761 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     2821 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     2881 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     2941 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     3001 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     3061 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     3121 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     3181 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     3241 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     3301 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     3361 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     3421 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     3481 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     3541 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     3601 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     3661 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     3721 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     3781 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     3841 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     3901 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     3961 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     4021 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     4081 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     4141 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     4201 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     4261 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     4321 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     4381 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     4441 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     4501 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     4561 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     4621 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     4681 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     4741 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     4801 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     4861 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     4921 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     4981 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     5041 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     5101 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     5161 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     5221 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     5281 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     5341 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     5401 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     5461 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     5521 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     5581 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     5641 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     5701 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     5761 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     5821 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     5881 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     5941 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     6001 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     6061 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     6121 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     6181 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     6241 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     6301 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     6361 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     6421 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     6481 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     6541 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     6601 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     6661 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     6721 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     6781 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     6841 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     6901 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     6961 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     7021 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     7081 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     7141 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     7201 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     7261 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     7321 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     7381 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     7441 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
     7501 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
     7561 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
     7621 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
     7681 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
     7741 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
     7801 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
     7861 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
     7921 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
     7981 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
     8041 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
     8101 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
     8161 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
     8221 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
     8281 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
     8341 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
     8401 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
     8461 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
     8521 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
     8581 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
     8641 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
     8701 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
     8761 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
     8821 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
     8881 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
     8941 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
     9001 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
     9061 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
     9121 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
     9181 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
     9241 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
     9301 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
     9361 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
     9421 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
     9481 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
     9541 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
     9601 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
     9661 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
     9721 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
     9781 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
     9841 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
     9901 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
     9961 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    10021 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    10081 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    10141 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    10201 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    10261 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    10321 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    10381 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    10441 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    10501 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    10561 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    10621 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    10681 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    10741 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    10801 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    10861 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    10921 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    10981 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    11041 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    11101 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    11161 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    11221 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    11281 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    11341 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    11401 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    11461 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    11521 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    11581 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    11641 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    11701 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    11761 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    11821 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    11881 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    11941 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    12001 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    12061 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    12121 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    12181 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    12241 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    12301 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    12361 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    12421 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    12481 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    12541 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    12601 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    12661 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    12721 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    12781 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    12841 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    12901 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    12961 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    13021 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    13081 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    13141 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    13201 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    13261 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    13321 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    13381 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    13441 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    13501 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    13561 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    13621 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    13681 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    13741 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    13801 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    13861 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    13921 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    13981 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    14041 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    14101 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    14161 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    14221 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    14281 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    14341 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    14401 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    14461 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    14521 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    14581 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    14641 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    14701 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    14761 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    14821 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    14881 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    14941 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    15001 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    15061 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    15121 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    15181 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    15241 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    15301 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    15361 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    15421 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    15481 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    15541 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    15601 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    15661 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    15721 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    15781 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    15841 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    15901 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    15961 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    16021 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    16081 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    16141 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    16201 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    16261 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    16321 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    16381 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    16441 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    16501 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    16561 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    16621 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    16681 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    16741 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    16801 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    16861 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    16921 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    16981 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    17041 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    17101 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    17161 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    17221 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    17281 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    17341 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    17401 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    17461 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    17521 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    17581 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    17641 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    17701 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    17761 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    17821 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    17881 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    17941 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    18001 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    18061 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    18121 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    18181 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    18241 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    18301 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    18361 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    18421 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    18481 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    18541 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    18601 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    18661 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    18721 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    18781 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    18841 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    18901 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga
    18961 tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa
    19021 agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta
    19081 gtg
//
LOCUS       DARDHD010000038        19065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000038 DARDHD010000000
VERSION     DARDHD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.038"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            184..516
                     /locus_tag="SDA06_001055"
     CDS             184..516
                     /locus_tag="SDA06_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228345.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            503..2506
                     /locus_tag="SDA06_001056"
     CDS             503..2506
                     /locus_tag="SDA06_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG4228346.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            2525..2995
                     /locus_tag="SDA06_001057"
     CDS             2525..2995
                     /locus_tag="SDA06_001057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG4228347.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3016..3600
                     /locus_tag="SDA06_001058"
     CDS             3016..3600
                     /locus_tag="SDA06_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG4228348.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            3615..4601
                     /locus_tag="SDA06_001059"
     CDS             3615..4601
                     /locus_tag="SDA06_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG4228349.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            4591..4902
                     /locus_tag="SDA06_001060"
     CDS             4591..4902
                     /locus_tag="SDA06_001060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG4228350.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            4924..6705
                     /locus_tag="SDA06_001061"
     CDS             4924..6705
                     /locus_tag="SDA06_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG4228351.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            6698..8074
                     /locus_tag="SDA06_001062"
     CDS             6698..8074
                     /locus_tag="SDA06_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG4228352.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8081..8716
                     /locus_tag="SDA06_001063"
     CDS             8081..8716
                     /locus_tag="SDA06_001063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG4228353.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            8746..10101
                     /locus_tag="SDA06_001064"
     CDS             8746..10101
                     /locus_tag="SDA06_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG4228354.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(10293..11687)
                     /locus_tag="SDA06_001065"
     CDS             complement(10293..11687)
                     /locus_tag="SDA06_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4228355.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            11779..12750
                     /locus_tag="SDA06_001066"
     CDS             11779..12750
                     /locus_tag="SDA06_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG4228356.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            12747..13529
                     /locus_tag="SDA06_001067"
     CDS             12747..13529
                     /locus_tag="SDA06_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG4228357.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(13698..14069)
                     /locus_tag="SDA06_001068"
     CDS             complement(13698..14069)
                     /locus_tag="SDA06_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228358.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            14203..14646
                     /locus_tag="SDA06_001069"
     CDS             14203..14646
                     /locus_tag="SDA06_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG4228359.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(14689..15498)
                     /gene="yidA"
                     /locus_tag="SDA06_001070"
     CDS             complement(14689..15498)
                     /gene="yidA"
                     /locus_tag="SDA06_001070"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG4228360.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(15517..16884)
                     /locus_tag="SDA06_001071"
     CDS             complement(15517..16884)
                     /locus_tag="SDA06_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4228361.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(16868..17698)
                     /locus_tag="SDA06_001072"
     CDS             complement(16868..17698)
                     /locus_tag="SDA06_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG4228362.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            17861..18298
                     /locus_tag="SDA06_001073"
     CDS             17861..18298
                     /locus_tag="SDA06_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG4228363.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            18374..18994
                     /locus_tag="SDA06_001074"
     CDS             18374..18994
                     /locus_tag="SDA06_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG4228364.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         6094 a         3274 c         4149 g         5548 t
ORIGIN      
        1 taatttgtta taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc
       61 tagttcttca aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc
      121 tttagaggta catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag
      181 gaagtgaagc aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgag
      241 aagcgtgctc gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa
      301 ttggagataa tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa
      361 caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag
      421 gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc
      481 attgaaaggg tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc
      541 agataaaaga aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat
      601 ccgggatctg tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac
      661 cacgattatt gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca
      721 agaagctatc caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa
      781 acgtcaagaa ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa
      841 taaattagaa gaagtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga
      901 tcgtttagaa gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag
      961 tatgtatgca agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga
     1021 aagtttcaag gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac
     1081 gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga
     1141 aattgctggg aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga
     1201 aaaattgcaa gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac
     1261 gacagcgtta ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac
     1321 acttgccgtt gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat
     1381 cgttttgcaa ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga
     1441 aactgtatca gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca
     1501 gcaggaagtc cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac
     1561 tgaaatgtac agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt
     1621 ttatcttgtc ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat
     1681 tgggacaata ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa
     1741 attctttgag attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt
     1801 cggacaggca ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac
     1861 gacggatgac gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt
     1921 ggttggatta ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt
     1981 cagtgatggt tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt
     2041 catgttgata aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc
     2101 tttatgtatc ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa
     2161 aggagcgtat aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg
     2221 tttgatggca ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc
     2281 tttcatgccg ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc
     2341 actgaatatg tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt
     2401 cgaatttttt ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc
     2461 ggaaaaatac gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta
     2521 tacaatgatg gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat
     2581 ggcaacagcg acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga
     2641 ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca
     2701 attgcttcca ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa
     2761 cttaggaaat gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat
     2821 tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca
     2881 aattttagcg aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga
     2941 aacatatgcg attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata
     3001 aaggaggaaa agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca
     3061 gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa
     3121 caaaaacgta cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta
     3181 cttgaaaaaa aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat
     3241 ttgaatgcga agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag
     3301 aattgggatg aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg
     3361 acaggcaaag taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg
     3421 ctggatgaat ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct
     3481 gatcaagcgg gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc
     3541 cttgtacaag atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa
     3601 ggagggatca gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg
     3661 aacttttgtc caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg
     3721 gtgaaatatt gaaatccacg atttacagtc catatattta tgacggatat gaatatgact
     3781 tcgaacagaa tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac
     3841 cggagcctga aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt
     3901 tgacaaaagc cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt
     3961 attcattaga aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa
     4021 gcattatgga cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg
     4081 gaattgacgt gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc
     4141 taggatatca agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa
     4201 cgatggctcg aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca
     4261 gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt
     4321 acacgaattt tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg
     4381 gaaatcttga tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc
     4441 aagaagcaca aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag
     4501 aggtagaaag caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaaccag
     4561 aaacaatcag aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac
     4621 aaagattctg ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag
     4681 caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc
     4741 acagagcaat gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata
     4801 cctgctatcg tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt
     4861 caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa
     4921 attttgcaaa ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg
     4981 tctgatgcca gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt
     5041 atcgagatgc gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt
     5101 ccaggcgaac cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc
     5161 atttcacaaa tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa
     5221 agtaatttct tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg
     5281 ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta
     5341 gaagaaacaa aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc
     5401 aaaacaatcg aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa
     5461 aatggtgaca aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc
     5521 aaagaaaaat taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc
     5581 ttcccagtga caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca
     5641 gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt
     5701 ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct
     5761 aatacaggag aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca
     5821 gtagcagctc gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac
     5881 atgggttatg acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt
     5941 gagatgagcg gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt
     6001 tcacggcttg cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt
     6061 cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa
     6121 ccagtcacac aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta
     6181 gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca
     6241 gaagtaggga agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag
     6301 ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt
     6361 gattcccttt cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat
     6421 tatttgcagc aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc
     6481 aaaatgttga agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt
     6541 gcttatttaa gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc
     6601 aaatatatcc cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa
     6661 acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat
     6721 cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga
     6781 attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca
     6841 agaagataaa gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc
     6901 ttctgtacga tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg
     6961 agtattcgat ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca
     7021 tttggatatt aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat
     7081 ccaaacaggt atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc
     7141 agtattttct ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc
     7201 aacggtactc gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt
     7261 tgaagaagct gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt
     7321 catgtttatg aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc
     7381 tttgactgct gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac
     7441 agacatgacc aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc
     7501 aggaagaaga ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc
     7561 aggaagaatc cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga
     7621 agacgataaa actcatccaa tccctgactt gactggttat attacagaag gacagatcat
     7681 cttgactaga gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct
     7741 ttcgcggtta aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac
     7801 gatgaatcag ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt
     7861 tttaggtgaa tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt
     7921 tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga
     7981 tctagggtgg gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga
     8041 catgctggac aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa
     8101 tccgacccgc atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca
     8161 taaattatta aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa
     8221 gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt
     8281 attggcaaat gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga
     8341 aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa
     8401 ttttgaatat gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag
     8461 tgccgaactg gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact
     8521 gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg
     8581 agtcaatgca ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg
     8641 tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat
     8701 gggtaccact gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc
     8761 aaacaaataa agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg
     8821 attttagttg gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca
     8881 aattttatcg atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca
     8941 ttgaatacag cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag
     9001 atcggcggtc ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa
     9061 gtcagtctca gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt
     9121 gtgatgaagc tcatgcttta catcctaaaa ttggcctttg ttatccaatt gattggtacg
     9181 gttgctttat cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt
     9241 ttgttccatg caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta
     9301 gccaatcacc agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt
     9361 gggttaggct tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg
     9421 attcattcaa aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg
     9481 ttctttttca cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg
     9541 gcaaacacct tttttatgag tgtgacacct agaacggctg gatattattc agttgattac
     9601 ctccatatga gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca
     9661 tctgggtcga cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg
     9721 cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc
     9781 gcagtgttgc gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc
     9841 atgattttat cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt
     9901 gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact
     9961 gcaataggga aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact
    10021 gtggtcttct ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa
    10081 gaaagtgtca tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata
    10141 agaaaataaa ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt
    10201 ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc
    10261 aataagattt gattccttta tttatcaata ggttataatt catatttttt tattttttct
    10321 tttacgatac ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga
    10381 acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg
    10441 ttagcaagaa agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt
    10501 cgatagtaag ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga
    10561 ttggctcttt ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg
    10621 aggatttgag tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt
    10681 gcttgttcta tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca
    10741 gaacaaataa attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg
    10801 aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca
    10861 ataacccata gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag
    10921 atttgaaacc ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt
    10981 tttctacatt cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga
    11041 acgatctcta aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct
    11101 gttgctattc tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga
    11161 acaccggaaa caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa
    11221 tttgctgcag tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca
    11281 acaagatttg ggttattact agttgattga ataaacggga caacgaattt attacattct
    11341 tcaaaagaac taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg
    11401 gtgatggact caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc
    11461 aaacctttct caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa
    11521 taatcagcta gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa
    11581 atatcttgtt ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg
    11641 tctggtcgcc aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct
    11701 taaaattgta taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt
    11761 gtgaggaggg aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt
    11821 tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc
    11881 agccaaaatc ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg
    11941 atgatgcctg cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt
    12001 ccggataatc atcagtatca tttaccttct catttaggag agattttctt gtttgatgct
    12061 caaaacggac tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct
    12121 gctgtatctg cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta
    12181 attggagctg gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc
    12241 actacagtgt ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct
    12301 aaaaaacagt atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt
    12361 ccagcggaaa tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg
    12421 gtaagggaag acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata
    12481 agtacatcgt tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca
    12541 gaaagcgggg acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt
    12601 ggtacattag gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg
    12661 acgattttcg acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat
    12721 aacaaattag agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg
    12781 cagatgaggt gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag
    12841 aaggtacgat gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga
    12901 agaaaaacca agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg
    12961 gtcacgcgac attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa
    13021 cgattaaatt ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt
    13081 acatggattt tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag
    13141 cgattggggc taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag
    13201 atgaaaaaac ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag
    13261 gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct
    13321 ttccaaaatt aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc
    13381 cttattgggc agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg
    13441 gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt
    13501 tgttttctaa aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt
    13561 ggagagtggc aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt
    13621 taatgaaaag tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt
    13681 aaaatgcccc atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg
    13741 acaaagtagt aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagaccg
    13801 gcagctttat gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca
    13861 tagccgtctc ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag
    13921 tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg
    13981 cggctttctt caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc
    14041 actcgaacac atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat
    14101 cataacaaaa agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg
    14161 taaaatggat agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt
    14221 ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa
    14281 agagatcagc agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg
    14341 gaaattgaag gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg
    14401 ggaattgtgg cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat
    14461 tatcgtgcaa cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt
    14521 ataggagaac caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt
    14581 gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa
    14641 aactgaaaga aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata
    14701 ttttttgatt gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc
    14761 ttctttgaca agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat
    14821 ggataagtcg ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg
    14881 ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta
    14941 atagggcgca cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat
    15001 tgctgcatct aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg
    15061 tgtcatttct tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat
    15121 cgtataagcg ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag
    15181 atgactgccg acacggcgtg ccatcacttc gatctctagg aaatcttcat gtgtcagtcc
    15241 gtgacgggcg atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat
    15301 aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa
    15361 gggacggcca gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc
    15421 ttttacttct tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc
    15481 gactagtttg atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc
    15541 attacggata tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg
    15601 caaggacgga tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc
    15661 cgctaatgca gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg
    15721 caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag
    15781 atcgtagcta gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa
    15841 caaagtgagt tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag
    15901 tggtttacga ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga
    15961 ccattgagta aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt
    16021 aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc
    16081 ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg
    16141 ggaaacagga tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc
    16201 cactgcatgc atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg
    16261 taatattcgg gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag
    16321 caaatagtcc attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt
    16381 ggggtaagtt ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt
    16441 aagaatctga tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg
    16501 attcgtatgg aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa
    16561 cagtgccgca cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt
    16621 ttcttttgaa aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc
    16681 taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa
    16741 ttgtttaatg cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg
    16801 atgctggaca tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat
    16861 ttgatattta taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc
    16921 tctggatttg gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat
    16981 cctgtaaatc acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt
    17041 cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca
    17101 ctgcaggata gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca
    17161 tgacttgccc gcgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc
    17221 catttttgat ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat
    17281 aagttccagg acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag
    17341 cgtatttagt caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt
    17401 cactttgggg gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc
    17461 cacctgaatt tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac
    17521 ttagaaaagg agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga
    17581 aatgtaaaaa gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa
    17641 aaggggaaaa gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc
    17701 aaccggctcc tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt
    17761 tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac
    17821 taggaacaat gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac
    17881 accgatttca atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta
    17941 ttttgatttg aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga
    18001 gattcaggag gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca
    18061 tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg
    18121 gttagaaaca tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga
    18181 actgcatgtt agtttgaaag accgtcccac aggagggaag gttcgaatcg attatgatct
    18241 gttcatggga ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat
    18301 ataaaagaaa agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa
    18361 ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat
    18421 gatcgaagta gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt
    18481 agctaaccag atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc
    18541 acaattttat actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg
    18601 gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga
    18661 cgaagaggat gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa
    18721 tgatgatgaa gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga
    18781 agaagatgac gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta
    18841 taattctgac ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc
    18901 aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga
    18961 agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg
    19021 acagagacga tcagcctatc agaataagga ggtgtgacac gattt
//
LOCUS       DARDHD010000039        19057 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000039 DARDHD010000000
VERSION     DARDHD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.039"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(72..1640)
                     /locus_tag="SDA06_001075"
     CDS             complement(72..1640)
                     /locus_tag="SDA06_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4228365.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1644..1925)
                     /locus_tag="SDA06_001076"
     CDS             complement(1644..1925)
                     /locus_tag="SDA06_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4228366.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1922..2845)
                     /locus_tag="SDA06_001077"
     CDS             complement(1922..2845)
                     /locus_tag="SDA06_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG4228367.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2842..3720)
                     /locus_tag="SDA06_001078"
     CDS             complement(2842..3720)
                     /locus_tag="SDA06_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG4228368.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3707..5044)
                     /locus_tag="SDA06_001079"
     CDS             complement(3707..5044)
                     /locus_tag="SDA06_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4228369.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5047..6150)
                     /locus_tag="SDA06_001080"
     CDS             complement(5047..6150)
                     /locus_tag="SDA06_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4228370.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6224..7732)
                     /locus_tag="SDA06_001081"
     CDS             complement(6224..7732)
                     /locus_tag="SDA06_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4228371.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7698..8390)
                     /locus_tag="SDA06_001082"
     CDS             complement(7698..8390)
                     /locus_tag="SDA06_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4228372.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8412..9386)
                     /locus_tag="SDA06_001083"
     CDS             complement(8412..9386)
                     /locus_tag="SDA06_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228373.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9686..10558
                     /locus_tag="SDA06_001084"
     CDS             9686..10558
                     /locus_tag="SDA06_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228374.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11140..13338)
                     /locus_tag="SDA06_001085"
     CDS             complement(11140..13338)
                     /locus_tag="SDA06_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228375.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13570..14562
                     /locus_tag="SDA06_001086"
     CDS             13570..14562
                     /locus_tag="SDA06_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4228376.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14622..16043
                     /locus_tag="SDA06_001087"
     CDS             14622..16043
                     /locus_tag="SDA06_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG4228377.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16036..17535
                     /locus_tag="SDA06_001088"
     CDS             16036..17535
                     /locus_tag="SDA06_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG4228378.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17574..18968
                     /gene="uxaC"
                     /locus_tag="SDA06_001089"
     CDS             17574..18968
                     /gene="uxaC"
                     /locus_tag="SDA06_001089"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4228379.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5730 a         3882 c         3366 g         6079 t
ORIGIN      
        1 atatatacaa acaaaaaaat tcactaacta gtgaaaataa gttagtgaac ctcatttatt
       61 aaagaattaa attacagatg atcttttcca tcatacttga aaaactcaaa atcagcataa
      121 cttcctcttt ttttatttag atcatgcact gctatcccga taaaatttcc agtaaaccct
      181 cctgagagta tacacatgtt tttttgcgga cctactttct tccattcttc agttgatacg
      241 tctttcaaat gaaactgtgc tgtctctttc tttacagtga ctttcaaaaa agtcttcttt
      301 tctgtgagtg gaatcgtcca atcttctatt tgtgctactc cgttttctga ttctatcata
      361 cgtaggcatt ttcccttgtc ctcattccaa gtaacataac aataaatata attggattca
      421 tttaagtaca gtaccagtcc agccatctga ttaaaattat taggcatgta ttcaaatgct
      481 gtctccgcat caaaagtaaa atccgtttgc cgaatagcta acaaatgatg ttcgaaggta
      541 gattgaagag attcaccgga aatcagacgt aaaaaacctg gtctacattt tgtatcacac
      601 caatcagcat ttggcaaaat gcgtctggca ttccagttct tctttagctc tccttcaaat
      661 gtatccataa actgatgatc tttttcttgt tgtacctctt catttgtctc aatcattatt
      721 tcttccatcg gagttgtctg gctatcttta agtcttaacc aatcgtcgga cgtccaatac
      781 acttcttgaa tagcggtttc tcttcccaaa atagccgcac cgtttaatgg tctggtacaa
      841 agatatgtca tgtaccatct tcctgacggg gtttgtacta agctcccatg tcctgtacat
      901 tgcaatgtag aagcaggttt gtcactagct gtcagcatag gaaaaccagg atcaagctcg
      961 taaggaccag taatattctt tgctcggcaa actgtaactg aatgtcctct tcctgtaccg
     1021 ccttctgccg tcatcaaata ataatacccg ttacgaaaat acaaatgtgg tgcctccgtt
     1081 ttcgctaatt ctgtacctgc aaatatttta tagatgggac catcaagctt cttcttctct
     1141 gcatcatatt cttgtaatac gataccgctg gatttattcg gtgtgtccag acgataatcc
     1201 catagttcat tgacaagcca tttttttccc gtttcaagat catggaacaa agaaggatca
     1261 aagccgttac tatttaaata aattggttcg gaccaaggac ctgtgatctc agatgcagtg
     1321 atcaaataat tgtggacatc tttaaacgga cggcctgcac ttttgacatc tgtgtaaaca
     1381 agataaaaca atccatcagc ataactgatc tgtggcgccc atatacttcc atttgtagga
     1441 tttccttgca aagatacttg agtggtcaaa atatccgttg tatgtgacca gttgactaaa
     1501 tttttcgact catatactcg gattcctgga agccattcaa aagaggagac aataatgtaa
     1561 taccgctctt tcactcttaa tatattagga tctggattga atcctcttaa aataggattt
     1621 ttcactttga tagtctgcat gattcaggct ccttttctca ttcttactcg atataaactg
     1681 aatgccccag ctgctaaaaa agcaagtgaa ataaacaaca caacggcaaa cagaccagtc
     1741 atgtcctgta ataatccaaa tgtattttct aaagacaaag ttgaatgatc tacagcaagc
     1801 gactgataac tttttttaaa ctctgacgca gacatattac ttaaagtaac tgctaacccg
     1861 ccaaagaaaa tcaaagatag actgccaccg accattccta gcaatagttc ccattttctt
     1921 ttcatttctg aaccccctgc ttttctaaat gtatccattc aggtcttttg atcgatgttt
     1981 cataattgtt attacgttca gagaatttga cagtctgttc atttttcttg tcatcccaat
     2041 tatcccaacc agttgggtga acatgcccac ctatcttaca cgcttcaaat aagacatttg
     2101 catacggacg ccacggtctt cctaaataga cgtttctagt atcaggttct gcagtcaagg
     2161 tacagctttc aaaacgatac cctgtttttt gatttttcgg cgtagatgct gctgtgatgt
     2221 atccaggatt tttactggct aagctatgga tatgacactg atgaaaaacg gcatctgccc
     2281 caccaaatat gaaatccacc gttccttcaa tccagcaata ttcataggtc tgacgacaat
     2341 actcaggatt ggcagaagcc ggtgtacaaa aaggagtacc atctttttgc aaatcaggca
     2401 acggacccgt gcataaggta tcctgataac cagatagtcg acagtttttt aaagatacgc
     2461 gatctcctga attgaataaa gcaacagcct gtcctacctt ttctcctgga cctgctgtat
     2521 tttcgatgtg aagattttct aatacgacat tcgatccttc taagaaaaaa gtagcacttc
     2581 ggaaagtccc taactggcta ccgtcaagat actcctgttt tgcaaaacga ttaccggaga
     2641 taactactgt tcctgctccc acaagtttaa aattggataa tctgctttct atatattcat
     2701 catatcttcc ttcttttact atcaagacct ttggatggct atccggcagg cctgccaaat
     2761 aatcgattcc ttcttggata gtttcaaaat ccgtcggggt atctatagca ttctttccaa
     2821 ttatcagttt gtctttcatc ctcataaatg ctcccttatg aattgtttcc aaatatttct
     2881 catttcttgt gtttccatat gaccgcctgt taaagaaaaa gaagaaaagt tttctggact
     2941 acccattgat tgatactttt tgtccagccc ttttctcagc ttcagtacgc cttcgtccaa
     3001 gcacatcgta tcgttttttc caactaagct aagacgaggg cgcggagcga ttttttcttg
     3061 gattgctaac gttgtataag ctgtcaatag atttggcaca tagtaataaa acccatgatg
     3121 atctaggcgg cgatgtagca aaagtgtttc gatgtccacc tgtgccgcta gatcaataca
     3181 aactttgaca cgagaatcca atgctgtcac ccaccagctt aataagcctc ccatagaaag
     3241 accaatcgtt gcgagccgtt gagtatctgt gtcttccctt gtctccaaat aatcgatcaa
     3301 gctgatcaca tcataaatac gcattcccca taacgttttt cctgttaata aaaattgttt
     3361 gaacaactcg ctttcagaaa tacctccgcg ttcttcaaat ccccaagcat caatcgacca
     3421 aacagcatat cccattcctg tcagtacttc tgcaaaagat ggagaagcta agtaaggagc
     3481 accatggatc aattcacttt tcccgactga aaaatctccg ccgtgagaat gcaggtaaag
     3541 gaccgtcgga aacggtcctt ctttttctgg ataagcatag agggctggta ctgtttccaa
     3601 ttcatttaag tacaattgat agtattcaag cataaaaccg ttttgttttt ctcggtataa
     3661 tcgttttcca ctaggggtat gtctgctaga tggagcacct aaaaaatcat tcaactgcat
     3721 aagcaaagtt tttacgaaca accagttcat ccatattttc tgattgattt gattggaatt
     3781 tctcaatact gatttcttta gtattttcta atagaaatgc tggtccctcg tgattttcaa
     3841 tcgacatctg gttaaaggaa accttacccg caaagccaat ataaaatcct tgtctgctca
     3901 tttcctctat tcctgtcatc atagcaggaa caccgggaac tgcatcttgg gccatcgaga
     3961 catcgatatt gtcaaatgtg atttcttgta tgaattgttc cgctaatcca taaataaaac
     4021 ctgcagaagc atggacattc cttgccgtga tattcgaaaa atggatcctt ctgaatgctg
     4081 atgtccgttc atctatcgga taagcctttt tttcccatac atacggttct ttccctcgcg
     4141 gaccacagaa ataataaaga ttcagtataa atggacacat cacattatcc atgactatat
     4201 tggatactcg aatatcttca acgatcccgc ctcgacctcg tctagatttc aatcgtattc
     4261 ctcgatcagt ttcttggaag atacaatttg aaatggtaat attacgaata ctaccactca
     4321 tttcacttcc taatacgaca ccgccatgtc catggaccat cgtgcaattt gtgatcgtga
     4381 tattttcgca agcgatcctt tcgtacgtat cttcagtccc tgccttgatt gcaatacagt
     4441 catctcccac gtcaatatgg caattactaa tacgcacatt tttgcatgat tctggatcaa
     4501 ttccgtctgt atttggtgaa tccgctgggt tcaagatcgt caagttatca aacgttgcat
     4561 tactgcaaag aatcgggttg atcgtccaac ttggtgactg gatcaattta atatctttca
     4621 cagtaattcg atgacagtta tggaaactca tcaatttagg acgaggataa gcgagattat
     4681 ctggttcatt tctaaaagta tgccaccatt tttttccatt tccatctaat gtaccgaaac
     4741 cagtgacaga gatgttctct acattttgag catagataca ggaagcataa acttttcgat
     4801 ggactccttc ccaacgtgaa tgaacaacag gataatcttt tggatcgtca gagaatttca
     4861 gaaccgctcc tgcgcttaaa tgaagttcga cattgctttt taaaaataaa gctcctgtta
     4921 gaaattcacc tgctggaaca actacggtcc cgcctccgtc acttgctgca gcatcaatgg
     4981 cttgttgtat tgcttcagta ttcaattcat caattgatgc gccaaatgtc aaaatatcat
     5041 acatatttat aatgcctcca cttcaatcaa tgcttgcaag aatgcaccaa cgcctttttg
     5101 atcattacaa ataattggtt cactgatgta gtaagcatat gaaccatctc gctgatctgc
     5161 tccgcctaac cctgctactt gacagttttt attcaagttc agccagcctt cttttgtttc
     5221 cagaacaaat tcatcaagta atccttgatg tgatttcttc acttgtggaa gccattcttc
     5281 tttgttcagc agtcccaaac gtattccttt agccattgcg taaacaaaca tactactgcc
     5341 ggaagcttcc agataatttc ctttccgttt tgtttctgtt gttacttgat accatgtccc
     5401 gctgttttct tcttgatatg gaagtaatgc agccagtgtc ttttctagaa tttctttcag
     5461 caaagaacaa tctgtttctt catgaagtaa ttcataggta tctgctgcag ccatcaaata
     5521 ccagccaatc gatcggctcc aaaaatgatg ggatagacct gtttttggat cagcccatgg
     5581 ttggaccttc ttttcatccc atgcatgaat cagcaaattt gtttttggat cgaatagatg
     5641 tttataactg atttcaaact gtctcaaaac atcttggtaa tctttttctt cactaaaatt
     5701 ctggataaat tcagcataaa aaggagaacc catatataag ccatccaacc aaatttgata
     5761 aggataaatt tctttatgcc aaaaagctcc ttcggatgtg cgtggatggt ttgccaactg
     5821 ctgggaaagg agttctgctg cttttttata tttttcttcg cctgtttctt tgtacaataa
     5881 aaagaaaagc tttcctgtat ttacatggtc gatattgtat tcttcaacgc tgtagccacg
     5941 gatcgttcca tcttcttgca cgaaatagtc gatattgtct tttatgaaat caaaataaat
     6001 tttttcttgg gttcgttccc aaagtaattg gaacccttta agtacgacgc catagtcata
     6061 agaccatttt cctttatacc acttttcttc atataagcga ggtgttcttt ccatgatact
     6121 ttttgctaat ttctttgacc agttttccat tatttgctct cctttaaaaa ttaaaatagg
     6181 atttgggaca aataccaaga agtatttgtc ccaatcgtct atattactgt gccgctgctt
     6241 tgtatgcttc ctcgtactct tgtgtgattc gatcgcctcc ttgtgctttc cagtcttcta
     6301 cagctttttt gaatccatct tcgtcaagct gtcccatgat gtattgcacc gtagcatcaa
     6361 tgacgatttt ttcaatttct gatcctactt cattgttcgt cggtgaatcc aatggcacag
     6421 aaggatccat tacagcaaat tcttcatttt ctttgatcaa ttgattagag aattcttttt
     6481 cagggtctgt atctttgaaa gagaacgtca cttcactagg acgagagctt gataacggtt
     6541 gcacctcttg ttgccaaaga tccatatcga taaattcgat agaaccatca tcattcaatt
     6601 cataatgcgt tccttcgata ccgccagtca tcaatttata cacatcttct tctaaaagat
     6661 catcaacaaa ttgaagtaat ttcttcaatt gtgcttcgtc ttttacttga gatttgggaa
     6721 atgctagcaa gccgccaata ccatttcctt ctgaccaaac atgataaccg ccgccttcac
     6781 catctgagat cttgtttaca ggcactaatt ccatatcatc ttgaatacct tgacccatcg
     6841 tcttcagatt cttgatatcc accattccag tgtagattcc agttcttcct tgtgcaaatt
     6901 gttcttgctg atcagtcttt tgggtaactg caaagtcttg tgccaaatag ccattctcaa
     6961 atagatctct tgaaaaattc aaagctttca tatactcttc tgtatcaaat tctggagtga
     7021 atttgccatt atcgtctaca gcccagccat ttggtgctcc aaaataagaa gtgaaaagtt
     7081 taaagctggt ataacgaata tcactaggcg cacgatcgcc aaaaccaact gtgtcgtcca
     7141 caccatttcc atcaggatcg ttttctgtaa attgtttagc tacttcataa agctcatcca
     7201 tggtagttgg aacatctagt cctagattat ccaaccagtc ttttcgaatg actaagccag
     7261 cacgagcgta gtctttttgt atcggtacgc cgtacaaggc accgtcgata ctcgcagctt
     7321 cgattctatc ttcagaaatt tgacttaaat ttttatagtc ttttaaatat tctgatacat
     7381 cccagaatac gcctgatttt aacgcattgc ggacagaaga gtttttcaac atcgcaattt
     7441 gaagtgtaac gatatctcct agttcatcag aagccaatgc agttgttaat ctttcttctt
     7501 ttgaagcatc aggaatccag ttgaatgtca gttccgtatc ggttttttct tcgatcaagt
     7561 tcacaatcgt gtctgtaggc ggtgaagcat gatgcaaaat atttaaccaa gtgattttag
     7621 cttttccatc ttctgtctct gaagaactgt tacttgaact gctgccgcat gcggcaagtg
     7681 ctagaattcc tgcaaaacta agacctaatt ttacgagcgt tgcttttttc ataaattttc
     7741 tccttcactg ttttaataat atttttgttt tcttctgttt tttctgtata tgagttcttc
     7801 cattccaaat ggcaaaatat agaatttgct gtccacgtga ctgcggaaaa gaagagactg
     7861 ccaccgataa aggttaaaac acctggcatt gtttggatga tcatcagata aatccctacc
     7921 atacttaata tcaacactaa tgagtaatga agatgtgaga atccgaataa aaatgccatt
     7981 ttcattttga aaaaatatcc tttccaatca taatgagcca tgattgggaa aatatagagt
     8041 gctgagagca catatatgaa cgcagcaata tacaaagcga tcctaagata ccaattgtgt
     8101 atgaaagaaa gatccacaag taaaacaatc ccgatggcgc catatatcca gctcatcagc
     8161 aaggtttcac gatagttttc tttaaaatat gtgaagtacg ttttcgtcaa aggaaacgtt
     8221 tcttttgagc gcagccattt tctaatcact gcaaccatcg catacgatgc aggaccgaaa
     8281 gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca gaaaattcaa ataaaccaac
     8341 tgtaaaatac gatttagcct tccgtttatt ttatctaatg actgcagcat atctttccct
     8401 ctctttctat cttagtaaac gccgtcttct cctagctttt tggcaatctt attagctaaa
     8461 attaccataa acaatcctac gaccccttta aacaatccca ttgctgtact gaaacttaac
     8521 tgtccattct tcaatccagc tgtataaata taggtgtcga aaatttctgc aacgcttcga
     8581 ttcaatgaat tcaataacaa gtacatatgc tcgaacccta agtctaatgt ctgaccgatt
     8641 tttagaatca gcaaggtaat gattactgga cgaattgccg gcaatgtaac atgccatgtt
     8701 ttgcgtaagc gtcctgctcc atccatttct gcagcttcat acaactgtgt atcgactgca
     8761 gttatcgctg aaaggtagat gatcgtagac cagcccaact ctttccagat cacttgtccg
     8821 atatatactg tgcgtgtcca ttcaggggat gttagaaaac tgattttttc aaatcccatt
     8881 gttgcaagga attcattcaa tacccctccg tcaacattca aaaatacata agtaatcgaa
     8941 acaatgatta cccaagacat gaagtgaggg atgtaaatga tcgtttgaat cccgtttttc
     9001 aatcgtttgt tcgttacttc atttaatagt aaagacaaaa tgattggcat cggaaaaaac
     9061 acgaagatat ttaatccgaa caagatcaac gtatttctta ataatgtaaa gaatgtcggt
     9121 tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag gacttcccat tattcctaaa
     9181 aatggttgat aatcttgaaa agcaataacg agtcccccca taggaagata cctaaaaatc
     9241 aagaaataaa ggactcccgg cagaatcatc acatataaaa atttattcgt tttgattcct
     9301 gcaattattt tttttcgtct tctgagcttt tcttcttttt tcgatagcgt cagttgaaat
     9361 ggttcttgtt tctttacact ttccataagc ccctccttag ttttgtgaat gtttgctgct
     9421 tatgacttca tgatagataa ctatctgaat tacgtatata atcattttta tcgtaagtaa
     9481 aaacgcttac atagcaatga tttttgtgct atacaaaacg ataaagtgta ttttttcaca
     9541 aataaactac gcatagcaaa ccttctattt ttttctatct gacacttagc ataggaaatg
     9601 attatatacg taatctttca ccgatgataa attgaacata tagaaacaga aacatttaat
     9661 ttattttgag gaggagccta tctatatgaa aaagaaagtc tctataggag aaagaagttt
     9721 tacacttttc aacaatacgt ttctaatttt gctagctttg atctgtatcg ttcctttttt
     9781 aaatatcatc gctacatctt ttgcttctac acaagaagtc gtcgcgaaaa agtttatcct
     9841 ttttcctact accttttctt tagacgcgta tcgctacatt ctttctacgc cgaccatttt
     9901 tagagcactt gctgtttcga ttggtgttac aggtgtcgga acgatcgtca gtatgtgtgc
     9961 aacttcactg atggcctacg gtctttccag aaagtatttg tttggtcgag gtttcgtcaa
    10021 ctttcttgtt gtattttcta tgttgttcag cggggggatg attccaacgt tcttagttgt
    10081 tcgttctcta gggctggtca attcttactg ggccatgatc ctgcctgtgg ctgttaatgc
    10141 aatgaatatg atcattatga gaaacttttt ccaagccttg ccggatagtt tggaagaatc
    10201 agcaaaaatg gatggttgca cagattttgg tgtatttttc aaaattatgc tgccattagc
    10261 tttaccgtct attgctacta tctcgctttt ttatgctgtt acttactgga acacttatat
    10321 gactgctatt ttgtatatca acgattcatc taaatggccg atacagattc ttttacgcca
    10381 aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt gccgttgatg tgattccacc
    10441 agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca gtgcctatgc tgattgttta
    10501 tccttttgtt cagaaatatt ttgtcaaagg agcactcgta ggttctgtca aagggtgatt
    10561 atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc agcttcaaga aataagaagg
    10621 catttacgaa aattgcttct taaatttttg tgaaatttcg gcttattttt cgaagaatct
    10681 actcctggaa caccgtgtat cagaggttgt gaaagagacg ttcagccttg agcgatgata
    10741 aaaactctca caaacattgc gacgagtaac ccacaggagc aataagaagc gattttgaaa
    10801 aatagcttcc catatttcac aaaattatgc cttattgtcg atagcctgtc tcttgaacgc
    10861 catttattcg taaaaaagaa ctgtgacaag acttttgtca cagttctttt tgcgtgctta
    10921 gccttgaaat ttaactatcc ataaatacaa atactagggt tattctaact tcttttattg
    10981 cttgaatcag tccacaagaa aaacagcctc ttgattaaac ggcgtttatc ttgttgattc
    11041 tgcggcaata aaatcagcca gcagcaaaac atgaagtgct gttttttgct gtcctctttt
    11101 attgcttgaa tcagatcaca agaaaaacag cctcttgatt cacaaatatt tttttcgata
    11161 ttctcccggc gtaatcgatt ccttcttttt aaagaaacga ataaaatttt gcggatttcg
    11221 atattgcaat ctttctgcaa tctcttttac ggagaggtta gtctctttca accatttttt
    11281 tgctacctct aaacgatgat tttgaacaaa atcaccaaat ttttctcctg tttccttttt
    11341 aaatacatta cttaaatagt tagggttata atgcaaccta tcagcgatca catctaaaga
    11401 cagatcacga tcatattctg tgtagacgat atgcataata ttctccgaca aatttttaaa
    11461 ttcatgatca gtgattgctt gagagcattc tgtgattggc agaactaatt gttctaaaac
    11521 aagtttttct agttctttgg gatgataatt gttcagtgct tttacataaa tttgtttcat
    11581 gttatcaaag ttttttgaat ctactcccaa taactgtcct aattgaacga gttcattgat
    11641 caagcggacc gaaactactt cccgactcaa aggattttta ttttgtgcaa acaatgcatc
    11701 aatcaattca tgtactaaat tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa
    11761 tcgattttgt tcttctatcg gatatctaat aagtgttttt tcatgagcag caggaacgat
    11821 ttcctgataa aaaataattg aacttggacc agtatttaca cgataataca gggcttcttt
    11881 tgcccgatca accgattgct tgctttcatt cagcgtttcg aaccggttac tgatgcctat
    11941 actgatagtt aaattcaagt attcttttac acgtttttga atgagtcggc aatactccat
    12001 gacttttttg cttgcttctg catcatcttt atccattcga taaatagtgg cttgcatttc
    12061 ttcattcaaa acgatcggca gcattcgatt ttcctttggc acaatttccg tgatcatgtt
    12121 gttgatcgct aaaagtagca aatcacgctc cccaccgtga ctatcttcta acagatcgat
    12181 ttgaaccaaa gctgtataat agcagtcatt ttgagaacta taaccaaatt gctgcaaccg
    12241 ctgatctagt tctcgccttt ctactcgatt tctaaataaa ctaagtacaa atagcgtttc
    12301 cagctgtggc ttttgggtaa ctaagtgtgc acttaacgct tctttttctc ctatgatccg
    12361 actgactgat tgagcgacca aatctagttc tttcccactg aatccttttg atttaagatt
    12421 cagacgttcc tgtatttgag aaatcggacg ggcaaatctg tccgaaaata agtaggaaaa
    12481 aagaccaatg agtcctacca gtgttaaaga cactgcaact agcccgattc tcagattgcg
    12541 gatcattgcg ccaattgcac tagagtcaac ttctaatctg taaatccaat tattataatc
    12601 tgatttcagt actatatacg ttttatcttt ttctcttatc actttcagct gattgtaatc
    12661 tagatccacg tccgttaata cagttggttg ctctttattt tttactgtct gagtagtaaa
    12721 aaggttgcca acttcgtttt caatagaaag tatgtcttct ccactttcac caacaacttg
    12781 ttctattgta tgtttctcta tcgtagcaat tcccaatgca tatttttctt tttcaaacat
    12841 tggcaaagaa atcaccattt cgattccttt gtttttcttt tgccaaaata aattattttt
    12901 gttatttatg tacttctgtt gatacttttc aacttcctct tcggtcagtt gactcaacga
    12961 tccattgatt actccccatt taccagccaa actgactaaa tcataattat tatcatccat
    13021 aacaatcgtt tctataaaat ttagttcttt cctgatttca gaataagtgg agtaatctgt
    13081 atatgtcagc ggatgcttga atatgtccga gaaatttttg gtcgaaccat acgtagaaaa
    13141 cgaatattca atcgttctaa ttttccattc aacgttagtt ttaacttgcc tgagcaaatt
    13201 ctttttgtct gttaaataac tcgattcgat cgattcatag gtattctggt aaatgaataa
    13261 actaaatcct atgacaagta cggctcctaa taaaaacatc ggtaaaaata ttttataaaa
    13321 tactgtactt ttcttcattt tcttcctctc ctccttttaa aataaaaggc tttcattttt
    13381 ctgtaaaatt gatactcatc taattaaatg agcattcccc gctttcaaga atagcataat
    13441 taaaaataaa aatccaagta atttttaacg tgtgcaatct tttcatatca aagatctatt
    13501 gacttatcgt agttatcgta atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa
    13561 aatgatggaa tggtaacgat caaagatatc gctaaagcag ctggcgtttc tcatacgaca
    13621 gtttctcgtg ctttgaataa cagcagttta ataaaaaatg aaacaaaaca aaaaatacga
    13681 caactagcta aggaaatgaa ttattcgcca aactatagtg caaaaggttt agtgaatcaa
    13741 aaaaaatatc taatcggact atttttctca agtttgcata aaggaacttc atcaaatttc
    13801 ctagcagatt ccattgcagg catccattca gtattagaca caaattacag tttatctgtt
    13861 gaaagtatcg atgttgttga tttaacagaa atcaacctgc aacgctacga tggactgatc
    13921 gttatgagtc aaagtgatga tgatcagcct ttcatcgact atctcaaaga aaaagaattt
    13981 cctttcgttg ttgtcaatcg tcacatcaat gatccagcgg ttatcaacgt cactgccgat
    14041 gatgcggccg gtgttatgca ggcagtagat tacggtattg gatgcggaca tacgaagatc
    14101 gcttatattg gcggaaagaa aaattttcgt tcgacaaaag aacgggaaaa aggggtattg
    14161 aaaagtctaa aaaaagcgag catccctgtc aatctagcct attttttaac aggagattac
    14221 agcttagaaa gcggcctcca aaacatggaa tacctattat cactttctga cctgcctact
    14281 ctggtttttt gcggcaacga cgacatagcg attggtgcat tgagagctgt acatttagct
    14341 ggcctccgtg ttccccagaa catttctctg atcgggttcg atgattctcc agtcgtctcc
    14401 tatctcaatc cgccgcttac caccgtacgt aagcctttga aagaaatgtg tcaagaagga
    14461 acaaagcttt tactagattt gatcgaaggc aggacgatcg aacaatctaa aattgagctc
    14521 tccaccttgt tgatccttcg tgagtcgatc aattacttgt aaactacaag taattttata
    14581 taaaaatgtt aacgtgtgca taacacagaa aggaagttta tatgaaaaaa ctgacaaaag
    14641 aattagtgaa aaaaaacgaa gcacctgtaa aagttcttca gtttggtgaa ggcaatttta
    14701 tgcgtggatt tattgattgg cagattcagc agctgaataa ccaacaattg tttaaaggta
    14761 atgttgcaat cattcaacct ttagaacaag gtcttggaaa tatgatgaac cagcaagata
    14821 atttatatac ggtcatttta caaggactca gcaatggcga aatcgttgat acttccgaga
    14881 ttatcaccgt agtagaacaa atgatcaatc cttatgatga atgggatgct tatctatctc
    14941 tagcagaaaa tgacgacttg gcctatatcg tctccaatac aacagaggca ggaatccaat
    15001 tcgtggagac tgataattta acagatactc ccccacagag cttcccgggc aaactgacag
    15061 cctttttata ccgacgtttc aaattaaaca aagctggctt taccatcatt ccttgcgaac
    15121 tgattgacag aaacggcgaa cgtctaaaag aaatcatttt acgctacgca gaaaaatggg
    15181 atttagaacc tgcttttgtt gagtggttag aaaaagaaaa tgttttctgt tgtagtttag
    15241 ttgatcgcat cgtcccaggt tatcctcgcg aagatgcagg tgttttcaat caacggcata
    15301 actatgaaga tcaattgatg gtcaaagcag aaccattcat gctttgggtg atcgaagggc
    15361 ctaaagaatt ggagaatcaa ctaccattca aacaggctgg cttgaatgtc atcatcacag
    15421 atgatatgac tccttatcgt gaacgtaagg tgcatttgct taatggaccg catacagcga
    15481 tggtccctct tgctttgttg gcggaattgg aaactgtaga agaagtaatg aaagatcctt
    15541 tattctccgc atatgttgat cagttattca atctagaatt gattcctatg ctttctttac
    15601 ctaaagatga attagccatt tatgctgacc aaatcaaaga acgtttcttg aatccttttg
    15661 cccatcacaa attagaagcg atttcattaa atagtgtttc aaagttcagc acccgtctgc
    15721 ttccagtgtt caagaagtat atcgaagaac aaaatcaggt accaccgtta atcactgttt
    15781 cactagctgc gctacttctt atgtatcgag gcgatcaagt aaagccacat gatgatgaaa
    15841 agacgataag cgaatttact gatgcttggt ctgataatgg aacagctatt cctcgattat
    15901 tgcaaaacgc tgcgctttgg ggagaagatt tgtctcaaat accgaatgtg acagatacag
    15961 ttcaagaaat agctgcaaag atcgatcaaa aaggtgtccg gtcagtatta caagaaatga
    16021 aaggaacgat ggtccatgct tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac
    16081 agtgtggtcg tcgcattaac ccctattcca aaacaaacac aactgacgat tgatcagcaa
    16141 acgatcacca ctcttgaaga tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa
    16201 gctggagaca acgtgatcaa atatggctat ccaatcggtc atgtgacgac agacgtaact
    16261 gccggtcaat ggctgcacac tcataatgta aaaaccaatc tttctggtga attggattat
    16321 cgatatgaaa aagatgtcca tccaagtcat tacctttttg agaaccgaac atttcaaggt
    16381 tacttgcgaa aaaatggaaa agtagggatt cgtaatgatt tgtttttagt acctaccgtt
    16441 gggtgtgtga atggcattgc tgaactgatc gtcaaacagt tcaaggaaaa acatccagat
    16501 ctgggacagt ttgaccatat tacgatttta aagcatcctt atggctgttc gcagcttgga
    16561 aaagatcatc aaaatactcg agaaatttta gcggacgcag taaatcatcc caatgccgga
    16621 ggagttttag tatttggatt aggttgtgag aacaatacag ttcctgaatt caaaaaaatt
    16681 ttaggtgaat atgatgaaga acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag
    16741 atcgaacaag gtgtggctct attagaagag cttttaacag cggcggaaaa tgaccagcgc
    16801 atatcagtcc cgctctctaa gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta
    16861 tcgggaataa ctgctaatcc tttacttggt gcattttctg actacttgat tgctcaagga
    16921 ggcagcacga tcttaacaga agtgccagaa atgtttggcg cagaacaagt attgatggca
    16981 cgagcggaaa atgaagaagt ttttgactca atcgttgact tgatcaatga cttcaaacag
    17041 tatttcttgt cttacgggga accagtttac gaaaacccat ctcctggtaa taaagcaggt
    17101 gggatcacta cattagaaga taagtcttta ggctgcacgc aaaaatcagg aagctctcct
    17161 gtcgttgatg tgctacaata tggcgaaaaa atcagaaaac cagggcttag tttattgcaa
    17221 gctcccggga atgatcttgt agctgcctct gctcttgctt catccgattg ccagttggtc
    17281 ttgtttacca caggaagagg gactccgttt ggctcatatg tcccaacggt caaggtatct
    17341 acgaatacaa ctctttttga acgcaaaggt cattggatgg attttaatgc aggcgtctta
    17401 ttagaacaac caatggaagt aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct
    17461 agtggagaag aaacgaaaaa tgaacaaaat gacgttcgag aaattgccat ttttaaaaac
    17521 ggagtaacac tttgattttc ataaaacagt ataaaaagaa agaaggaaga tttatgtttt
    17581 tatctgatga ttttttatta aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa
    17641 aaaacatgcc cattattgat tatcactgtc acttagatcc aaaagaaatt tacgaaaaca
    17701 acaatttcag caatctgaca gaagcttggt taggaggcga tcattacaaa tggcgcttga
    17761 tgcgagcttg cggcgtgcct gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat
    17821 ttttagcttg gtgtcaaacc gtaccaaaaa ttatcggaaa tccgctttac agttggtcac
    17881 atcttgaatt gaagcgtttc ttcggtattg acttgttgat aaatgaagaa aatgcagaga
    17941 aaatttggac acaagcaaat gaaattttgg cagctcctga ttttcgccgt cgggaaatcg
    18001 caaaaaaatc gaatgtagaa gtgatttgta caacagatga tcccgcagac gacttgcact
    18061 atcatcagtt gcttgctcat gaagaaccag atttgaaggt tctaccttca ttccgtccag
    18121 ataaagcact caatattgaa aaagaaggat ttaatgcatg ggtcacacta ctggaagaag
    18181 cagctgacca aacgatcacc acctatcaag aactgataga agtcttaggg cagcggatag
    18241 aatacttcca tcaacatgga tgtcgtgttt ccgaccacgc tttagacatc ttacgctata
    18301 aagaagcgga tgaaacagta ttagaagaga tttttcaaaa agcacgagct aacaaactat
    18361 tatcttcaga tgaaatcgat gcttatctta cagaaacact gatacatttg acccacttct
    18421 atcattctca taattggacg atgcagctgc acatccatgc ttatcgtaac tgtaatacac
    18481 aaatgttgga aaaattaggg cccgataccg gctatgatgg aatgaatgat ctttctttga
    18541 ctattcctct gcaaaaatta ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga
    18601 tcctttattc attgaatccg aatgactatc ctgcgatttt agctttgatg ggctgtttcc
    18661 aaaaagaaca gaatggaaaa ctgcagttag gttctggatg gtggtacaac gatactcgag
    18721 ctggtatgcg cgatcaattg acacaatttg ctgatggtag tgctttgggg aattttgtcg
    18781 gcatgctgac agattcaaga agtttcctgt cctacactag acatgaatat ttccgtcgtg
    18841 ttctttgtga atttttaggc gaaatggtca tgcgaggaga agcacctgaa gatgaacaac
    18901 ttttaggaag tctagttcaa gcgattagtt atacaaatgc taaaaattac tttggctttt
    18961 ttgcctaatt gattaatagt aacagtaacg aatcaaaaac aggatagtga aataacggaa
    19021 tccgtttatc cacgttttag agaagaagac ggaccaa
//
LOCUS       DARDHD010000040        18357 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000040 DARDHD010000000
VERSION     DARDHD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.040"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..504
                     /locus_tag="SDA06_001090"
     CDS             22..504
                     /locus_tag="SDA06_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG4228380.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            541..1551
                     /locus_tag="SDA06_001091"
     CDS             541..1551
                     /locus_tag="SDA06_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG4228381.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1666..2379)
                     /locus_tag="SDA06_001092"
     CDS             complement(1666..2379)
                     /locus_tag="SDA06_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228382.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2569..3159)
                     /locus_tag="SDA06_001093"
     CDS             complement(2569..3159)
                     /locus_tag="SDA06_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG4228383.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3643..3870
                     /locus_tag="SDA06_001094"
     CDS             3643..3870
                     /locus_tag="SDA06_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG4228384.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3886..5052
                     /locus_tag="SDA06_001095"
     CDS             3886..5052
                     /locus_tag="SDA06_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG4228385.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5286..6329
                     /gene="hrcA"
                     /locus_tag="SDA06_001096"
     CDS             5286..6329
                     /gene="hrcA"
                     /locus_tag="SDA06_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG4228386.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6423..6986
                     /gene="grpE"
                     /locus_tag="SDA06_001097"
     CDS             6423..6986
                     /gene="grpE"
                     /locus_tag="SDA06_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG4228387.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7037..8866
                     /gene="dnaK"
                     /locus_tag="SDA06_001098"
     CDS             7037..8866
                     /gene="dnaK"
                     /locus_tag="SDA06_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG4228388.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9017..10183
                     /gene="dnaJ"
                     /locus_tag="SDA06_001099"
     CDS             9017..10183
                     /gene="dnaJ"
                     /locus_tag="SDA06_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG4228389.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10265..10930)
                     /locus_tag="SDA06_001100"
     CDS             complement(10265..10930)
                     /locus_tag="SDA06_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228390.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10906..11079
                     /locus_tag="SDA06_001101"
     CDS             10906..11079
                     /locus_tag="SDA06_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228391.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11124..11861)
                     /locus_tag="SDA06_001102"
     CDS             complement(11124..11861)
                     /locus_tag="SDA06_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4228392.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11863..13365)
                     /locus_tag="SDA06_001103"
     CDS             complement(11863..13365)
                     /locus_tag="SDA06_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG4228393.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13624..15618
                     /gene="uvrB"
                     /locus_tag="SDA06_001104"
     CDS             13624..15618
                     /gene="uvrB"
                     /locus_tag="SDA06_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG4228394.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15628..>18357
                     /gene="uvrA"
                     /locus_tag="SDA06_001105"
     CDS             15628..>18357
                     /gene="uvrA"
                     /locus_tag="SDA06_001105"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG4228395.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGG"
BASE COUNT         6036 a         3220 c         3943 g         5158 t
ORIGIN      
        1 aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata
       61 tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag
      121 aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat
      181 aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag
      241 atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa
      301 aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa
      361 gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc
      421 atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg
      481 ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac
      541 atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca
      601 ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa
      661 tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca
      721 gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc
      781 tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct
      841 ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca
      901 ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag aggagagtac
      961 ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt
     1021 ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc atcatcttct
     1081 gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg
     1141 aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg tactccttat
     1201 ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta
     1261 gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca
     1321 ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga ggatttgtta
     1381 gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg
     1441 tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg tgcctatgca
     1501 gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt
     1561 tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag tgaaagaagc
     1621 tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat tatctataac
     1681 tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag ctactacaga
     1741 tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc
     1801 tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact
     1861 tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg
     1921 atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac
     1981 atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag ctagccaaac
     2041 acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga
     2101 ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga
     2161 tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt
     2221 agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga ttgaactttt
     2281 gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa
     2341 taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt
     2401 ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ctatgaatat
     2461 aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa tcatcagtaa
     2521 aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt aggagaaagg
     2581 atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa tcgttttttc
     2641 tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga attctatagg
     2701 agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt ctataaaatc
     2761 ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc cggtgtgttt
     2821 gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa tagcagctac
     2881 tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat tttttatgtc
     2941 ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt gttcgattaa
     3001 gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga tagaagcagt
     3061 tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac cttcccaatc
     3121 ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct cctttactat
     3181 ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt agcgacagcg
     3241 aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa ttaaatacga
     3301 actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat tttgcataca
     3361 tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga aactggaagg
     3421 caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca tgaaaacaac
     3481 cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc aagaagtcga
     3541 ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta tatgagaggt
     3601 tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat agcttttcat
     3661 atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt ttatcgagag
     3721 gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga gctaggtttc
     3781 cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga ttttaagaag
     3841 gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata
     3901 aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga gcatatctgc
     3961 tattactgtg attttaataa agttttttta gaagggcagc cagtagatga gtatgtggaa
     4021 atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa tttagaaaca
     4081 ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga tcgactgtta
     4141 tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt agaagcgaat
     4201 ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt caatcgttta
     4261 tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg taaacacacc
     4321 gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac caatgtaagc
     4381 atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga tactttggaa
     4441 aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct cgaaaacaaa
     4501 acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca ggaaacggaa
     4561 accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca ccaatatgaa
     4621 ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta ctggaacaat
     4681 gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat tcgttaccgc
     4741 aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg cccggtattg
     4801 gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt aggactaaga
     4861 aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg
     4921 gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac
     4981 cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg
     5041 tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa aaaagttcaa
     5101 aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa ttatttagaa
     5161 ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca ggagaagggc
     5221 ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa tgagaggtga
     5281 ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa cactatacga
     5341 gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag gccagctcag
     5401 caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg aagactcact
     5461 cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat catttgttaa
     5521 aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta ggcagagaat
     5581 ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag ctgacaagct
     5641 atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc tttcgtatcg
     5701 tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg aatgtagaaa
     5761 gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa atggtacaga
     5821 ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa cttcgtacgg
     5881 agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct cagttatttg
     5941 acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa atgaatctct
     6001 tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct tttatgaaag
     6061 actcagacgg acttacacaa atgatcactc cgatggatag caatattcat atcaagattg
     6121 gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca agttacgaga
     6181 tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg ccttattctc
     6241 ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt gctgattact
     6301 accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg cagctgatcg
     6361 ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag gaagactgta
     6421 tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag ccagaaccgg
     6481 aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg gaagaattcg
     6541 aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg cgggctcgtg
     6601 ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc
     6661 gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa cgtgcattag
     6721 ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg
     6781 aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct aaaggagaag
     6841 cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa gatacaccag
     6901 ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc gtgttaagac
     6961 caaccatggt cattgttgca caataattgt gcggatataa aacttacact ttcaattaaa
     7021 gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga ctaactctgc
     7081 agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag gaaaccgtac
     7141 aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg
     7201 tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg gtgaagcagg
     7261 ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag caatgatcct
     7321 tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa aagcagttat
     7381 cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg ctggtaagat
     7441 cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg
     7501 tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg gcggtacttt
     7561 tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa cagctggcga
     7621 caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg tagcagaatt
     7681 caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac gcttgaaaga
     7741 cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa tcagcttgcc
     7801 atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga cacgtgcaaa
     7861 atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac gtcaagcgct
     7921 aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg gtggatctac
     7981 tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac caaacaaatc
     8041 agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg tgatcactgg
     8101 tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta tcgaaacaat
     8161 gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa gtaaatcaca
     8221 agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat tacaaggtga
     8281 acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag atattccagc
     8341 agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa acgggattgt
     8401 aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga tcaaatcttc
     8461 ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag caaatgcaga
     8521 agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg cattgctatt
     8581 ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa
     8641 agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca tcgaagaaat
     8701 gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta aactatacga
     8761 acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg gagcagatga
     8821 tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag ttcactgaat
     8881 ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag tttgaagtat
     8941 cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat gatgagaacc
     9001 aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag ggctctcaaa
     9061 aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc
     9121 tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag aagcatatga
     9181 gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg caggcacaga
     9241 tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg gcttctctgg
     9301 tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag gcggtcggtc
     9361 agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt
     9421 tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag aaatctgtca
     9481 tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc ataaatgtca
     9541 tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga tgagccgtca
     9601 aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc caacctgtca
     9661 tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag ctggtgtaga
     9721 agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg gcggaccatt
     9781 tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc gtgaaggttc
     9841 tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg atgaagtcca
     9901 agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac agacaggtac
     9961 gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca
    10021 acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt
    10081 acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag acggtttctt
    10141 cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct agtaaaaacg
    10201 ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg tttttttgta
    10261 gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg tcatagtttc
    10321 taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag gtgtttttat
    10381 gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg aagcatgcca
    10441 aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata cgttttacat
    10501 ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct aaatcttcaa
    10561 aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact tgtttggcat
    10621 gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt ccaaagttta
    10681 gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat gatagcaatg
    10741 aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca gtgagcaaag
    10801 cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg atccgttctt
    10861 gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga gaagaaaaat
    10921 caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa tgaaccgatg
    10981 gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg aaaacaagcg
    11041 atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt cccttcatgg
    11101 cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa atcttttgtc
    11161 cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa aaagtttccg
    11221 tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc gtgcgtaacg
    11281 ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac atcaccgacc
    11341 atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg gttcatcgct
    11401 agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc aggatacata
    11461 tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt ctctgcttct
    11521 tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa aatagaaaga
    11581 tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac caagttgata
    11641 tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc agaaggttct
    11701 tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg gccaatgata
    11761 cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt gttatcgccg
    11821 tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca ccttcttttc
    11881 taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga tcgcaatgat
    11941 caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag tttgcagcaa
    12001 ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa cgaactgatt
    12061 gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt gcattgtccg
    12121 attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa cggcgttgat
    12181 cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg tgataacacc
    12241 agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga aaataaagaa
    12301 cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag caattgtgcg
    12361 aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta ctatagccaa
    12421 agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca atgcttgata
    12481 gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact cactggaagt
    12541 ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg ttctttttaa
    12601 ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt ttttaacggc
    12661 aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt gtccttgttt
    12721 gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc caacttttaa
    12781 cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat attcagattc
    12841 atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg tttttccttt
    12901 caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt ggatcccgct
    12961 atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta cagtcattcc
    13021 ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag agaaaccacg
    13081 gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca attttacatc
    13141 gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag cagagtcact
    13201 ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc ttgtgcttga
    13261 ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaaatg tcacaagtcc
    13321 tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca acctcctaaa
    13381 tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact gtaagctcga
    13441 aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac atgtataata
    13501 agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa ggaatgcctt
    13561 tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa aggaggacga
    13621 agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata ccaaccggct
    13681 ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg gaaaaaagca
    13741 caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa tcttattgag
    13801 aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg tcagctgtac
    13861 ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc ttattatgat
    13921 tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa agattcaagt
    13981 gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt agaaagaaat
    14041 gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc ttttgagtac
    14101 caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca gttgattcgt
    14161 gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg tcgttttcga
    14221 gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc cttacgtgtg
    14281 gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac aggtgaagtt
    14341 ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac gaatgatgaa
    14401 catatggagc atgccgtagc gaatatcaaa gcggaattag aacagcgttt aactgtcttg
    14461 cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa ttacgatatt
    14521 gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg gcatatggac
    14581 ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga agattttttg
    14641 atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta taatggtgac
    14701 cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc tttagacaat
    14761 cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta tgtctctgcg
    14821 acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat catccgtccg
    14881 acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat cgatgatcta
    14941 gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac gacattgacg
    15001 aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa agtcaaatat
    15061 ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct tcgattaggt
    15121 gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt acctgaagtt
    15181 tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga acgttcctta
    15241 gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat gtacgcagac
    15301 aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg tacgattcag
    15361 cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga aatccgagat
    15421 ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca agtctcgtac
    15481 gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa agaaatgaaa
    15541 gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat gattttagaa
    15601 ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat tcatggtgca
    15661 cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt cgtggtcgtt
    15721 acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta cgctgaaggg
    15781 cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg acaaatggat
    15841 aagccggacg tagatagcat cgacggattg agtccggcga tttctattga tcagaaaaca
    15901 acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga ctatttacgc
    15961 ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga gattaccagt
    16021 caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac aaagatccaa
    16081 gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt cgaacgaatc
    16141 caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt aagtgaagtg
    16201 cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg gatcattgtg
    16261 aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg attagcagac
    16321 ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga acactatgcc
    16381 tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc attcaatgcg
    16441 ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt cgatcaagat
    16501 ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc atggaatcca
    16561 atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt cggtatcgat
    16621 ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt gaatgggaca
    16681 gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga tgtagaaacg
    16741 actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa cagtgatttc
    16801 acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg taaaggttat
    16861 cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg gcaaacaaat
    16921 gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc tgaacaagag
    16981 aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt cttggaaaat
    17041 gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg gggagaagcg
    17101 cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt gtatatttta
    17161 gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg ctcattaaag
    17221 aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga tacgatgtgg
    17281 gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg acaaatcgtg
    17341 gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg gaaatatctt
    17401 tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg gaaagcaatc
    17461 aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt tccattagga
    17521 gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt caatagtatc
    17581 ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg taaattcaag
    17641 acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag cccaatcggc
    17701 cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat ccgtgggtta
    17761 tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt tagtttcaac
    17821 gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat cgaaatgcac
    17881 tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata taattctgaa
    17941 acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat gacagtagag
    18001 gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca aacgattgtt
    18061 gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc cggtggtgag
    18121 gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa gaatttctat
    18181 atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt gttgcatgta
    18241 ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa tctagatgta
    18301 atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg tggagga
//
LOCUS       DARDHD010000041        17790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000041 DARDHD010000000
VERSION     DARDHD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.041"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..858
                     /locus_tag="SDA06_001106"
     CDS             46..858
                     /locus_tag="SDA06_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG4228396.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            855..1655
                     /locus_tag="SDA06_001107"
     CDS             855..1655
                     /locus_tag="SDA06_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG4228397.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1816..3084
                     /gene="rseP"
                     /locus_tag="SDA06_001108"
     CDS             1816..3084
                     /gene="rseP"
                     /locus_tag="SDA06_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG4228398.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3152..4861
                     /locus_tag="SDA06_001109"
     CDS             3152..4861
                     /locus_tag="SDA06_001109"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG4228399.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5073..9431
                     /locus_tag="SDA06_001110"
     CDS             5073..9431
                     /locus_tag="SDA06_001110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG4228400.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9576..10049
                     /gene="rimP"
                     /locus_tag="SDA06_001111"
     CDS             9576..10049
                     /gene="rimP"
                     /locus_tag="SDA06_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG4228401.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10072..11247
                     /gene="nusA"
                     /locus_tag="SDA06_001112"
     CDS             10072..11247
                     /gene="nusA"
                     /locus_tag="SDA06_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG4228402.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11269..11562
                     /locus_tag="SDA06_001113"
     CDS             11269..11562
                     /locus_tag="SDA06_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG4228403.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11559..11870
                     /locus_tag="SDA06_001114"
     CDS             11559..11870
                     /locus_tag="SDA06_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG4228404.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11883..14189
                     /gene="infB"
                     /locus_tag="SDA06_001115"
     CDS             11883..14189
                     /gene="infB"
                     /locus_tag="SDA06_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG4228405.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14216..14563
                     /gene="rbfA"
                     /locus_tag="SDA06_001116"
     CDS             14216..14563
                     /gene="rbfA"
                     /locus_tag="SDA06_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG4228406.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14720..14893)
                     /locus_tag="SDA06_001117"
     CDS             complement(14720..14893)
                     /locus_tag="SDA06_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228407.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15341..17703
                     /locus_tag="SDA06_001118"
                     /pseudo
     CDS             15341..17703
                     /locus_tag="SDA06_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
BASE COUNT         6077 a         3158 c         3911 g         4644 t
ORIGIN      
        1 aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt
       61 ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca
      121 attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt
      181 ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa
      241 gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag
      301 cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc
      361 gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg
      421 ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga
      481 atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa
      541 gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg
      601 attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg
      661 atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt
      721 gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca
      781 atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag
      841 aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta
      901 ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa
      961 taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt
     1021 tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc
     1081 cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat
     1141 cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct
     1201 tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt
     1261 tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac
     1321 gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc
     1381 tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa
     1441 aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag
     1501 tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact
     1561 cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg
     1621 tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga
     1681 atttcataaa agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt
     1741 atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta
     1801 aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg
     1861 attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa
     1921 tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat
     1981 acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa
     2041 acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag
     2101 aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt
     2161 tatgatttag aagatgagct gactatcact ggttatgtga atggagacga aacagaagta
     2221 gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga
     2281 attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac
     2341 tttgctggac ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt
     2401 atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga
     2461 gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa
     2521 atccattcat ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac
     2581 tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag
     2641 tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg
     2701 gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc
     2761 ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac
     2821 caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt
     2881 ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa
     2941 ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga
     3001 atattgacat tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac
     3061 gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata
     3121 actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac
     3181 attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg
     3241 atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt
     3301 ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct
     3361 gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg
     3421 accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca
     3481 cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt
     3541 aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct
     3601 tcgcggaaga gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt
     3661 ggacgagaca tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt
     3721 agaatttcgc tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt
     3781 catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc
     3841 tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct
     3901 tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt
     3961 ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt
     4021 ccttgttttg gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc
     4081 tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt
     4141 tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt
     4201 ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt
     4261 gaatccagaa cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg
     4321 agatccttca ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca
     4381 tatcttcaaa atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa
     4441 tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc
     4501 agcaatcgta gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc
     4561 atttgatttg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa
     4621 tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga
     4681 acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt
     4741 cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt
     4801 tgaagtacgt aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata
     4861 ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg
     4921 attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac
     4981 tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt
     5041 gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa
     5101 aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg
     5161 gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt
     5221 tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt
     5281 ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac
     5341 agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag
     5401 cacagcaagt attgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa
     5461 tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt
     5521 tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag
     5581 cggaacgagt attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga
     5641 agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat
     5701 cgccagctct tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac
     5761 caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg
     5821 tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca
     5881 cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag
     5941 tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata
     6001 cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc
     6061 gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga
     6121 gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac
     6181 accgagctat cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg
     6241 ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg
     6301 gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat
     6361 ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag
     6421 tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc
     6481 ctttgtcacg aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa
     6541 agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag
     6601 cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc
     6661 ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat
     6721 tcaaacgttt cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc
     6781 accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg
     6841 caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag
     6901 attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac
     6961 tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac
     7021 gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg
     7081 ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag
     7141 caaaattcta cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc
     7201 aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg
     7261 gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag
     7321 atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc
     7381 atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt
     7441 taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct
     7501 gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag
     7561 atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg
     7621 agatcattca aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg
     7681 ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag
     7741 gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta
     7801 atccgttagc ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg
     7861 gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct
     7921 taaacaaaga cggtcatgat attccatttg agacattttt aggattccat ggagacaaag
     7981 tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga
     8041 aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca
     8101 aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg
     8161 cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc
     8221 caggggggat catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat
     8281 atccagcaga cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc
     8341 acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc
     8401 ttcaagattt atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc
     8461 ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag
     8521 ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc
     8581 atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat
     8641 ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg
     8701 gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat
     8761 ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag
     8821 aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt
     8881 caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc
     8941 gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac
     9001 gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa
     9061 tgaaagaaat cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag
     9121 tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt
     9181 ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag
     9241 ctgtcccaag tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc
     9301 catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat
     9361 atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg
     9421 atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt
     9481 tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg
     9541 aattacgcac ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca
     9601 cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg
     9661 taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata
     9721 tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag
     9781 atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga
     9841 aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac
     9901 cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta
     9961 cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg
    10021 caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa
    10081 gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg
    10141 atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa
    10201 aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa
    10261 gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc
    10321 aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg
    10381 cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga
    10441 acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa
    10501 cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa
    10561 caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc
    10621 cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta
    10681 ttgcgccgct tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc
    10741 agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat
    10801 atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat
    10861 gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt
    10921 gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag
    10981 gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa
    11041 aacgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac
    11101 atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa
    11161 gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa
    11221 acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg
    11281 aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc
    11341 cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga
    11401 cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt
    11461 ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat
    11521 gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa
    11581 aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac
    11641 tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa
    11701 gcgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg
    11761 agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa
    11821 taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt
    11881 gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg
    11941 tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta
    12001 gcaatgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg
    12061 cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa
    12121 caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga
    12181 gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca
    12241 gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca
    12301 acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata
    12361 aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat
    12421 tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga
    12481 aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg
    12541 tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca
    12601 tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag
    12661 aagaagtcga tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg
    12721 tagaccatgg gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg
    12781 aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa
    12841 tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa
    12901 gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag
    12961 ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa
    13021 ttgataaacc aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa
    13081 ttcctgaagc atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa
    13141 acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag
    13201 atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc
    13261 ctgtgtcaac cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg
    13321 gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag
    13381 caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc
    13441 gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac
    13501 gtgcattatt agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa
    13561 gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt
    13621 cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa
    13681 tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca
    13741 acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata
    13801 ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa
    13861 ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg
    13921 ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag
    13981 gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag
    14041 gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg
    14101 aatgtggtgc aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat
    14161 ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc
    14221 taactaccgt gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg
    14281 caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg
    14341 ggacttacag caagcaacga tctattacag cattttatct gacttagcat cagataagaa
    14401 aacagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa
    14461 tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg
    14521 caaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa
    14581 tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag
    14641 ttccatgact ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa
    14701 aaattcgact ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga
    14761 taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa
    14821 ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta
    14881 aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg
    14941 atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat
    15001 attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg
    15061 atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc
    15121 atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat
    15181 caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg
    15241 ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc
    15301 tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga
    15361 atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact
    15421 ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca
    15481 tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg
    15541 agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca
    15601 agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac
    15661 ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc
    15721 ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc
    15781 tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat
    15841 tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat
    15901 caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc
    15961 caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact
    16021 tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc
    16081 tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa
    16141 gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg
    16201 atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca
    16261 agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct
    16321 gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag
    16381 agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat
    16441 cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc
    16501 aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga
    16561 gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa
    16621 tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga
    16681 atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg
    16741 gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc
    16801 ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac
    16861 ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag
    16921 caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag
    16981 aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg
    17041 gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt
    17101 gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt
    17161 gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg
    17221 gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta
    17281 aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg
    17341 cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt
    17401 gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat
    17461 ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta
    17521 gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt
    17581 caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt
    17641 cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa
    17701 taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa
    17761 aataaataaa aaaaacggaa aaaagactgg
//
LOCUS       DARDHD010000042        17555 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000042 DARDHD010000000
VERSION     DARDHD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.042"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..175)
                     /locus_tag="SDA06_001119"
     CDS             complement(<1..175)
                     /locus_tag="SDA06_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228408.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(654..1373)
                     /locus_tag="SDA06_001120"
     CDS             complement(654..1373)
                     /locus_tag="SDA06_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4228409.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1422..3068)
                     /locus_tag="SDA06_001121"
     CDS             complement(1422..3068)
                     /locus_tag="SDA06_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4228410.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3247..3837
                     /locus_tag="SDA06_001122"
     CDS             3247..3837
                     /locus_tag="SDA06_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4228411.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3929..5434
                     /locus_tag="SDA06_001123"
     CDS             3929..5434
                     /locus_tag="SDA06_001123"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG4228412.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5518..6870)
                     /gene="celB"
                     /locus_tag="SDA06_001124"
     CDS             complement(5518..6870)
                     /gene="celB"
                     /locus_tag="SDA06_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4228413.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6870..7388)
                     /locus_tag="SDA06_001125"
     CDS             complement(6870..7388)
                     /locus_tag="SDA06_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228414.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7404..7730)
                     /locus_tag="SDA06_001126"
     CDS             complement(7404..7730)
                     /locus_tag="SDA06_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG4228415.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7743..9722)
                     /locus_tag="SDA06_001127"
     CDS             complement(7743..9722)
                     /locus_tag="SDA06_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4228416.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9743..10057)
                     /locus_tag="SDA06_001128"
     CDS             complement(9743..10057)
                     /locus_tag="SDA06_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG4228417.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10225..10518
                     /locus_tag="SDA06_001129"
     CDS             10225..10518
                     /locus_tag="SDA06_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG4228418.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10596..11708)
                     /locus_tag="SDA06_001130"
     CDS             complement(10596..11708)
                     /locus_tag="SDA06_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4228419.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12095..12274
                     /locus_tag="SDA06_001131"
     CDS             12095..12274
                     /locus_tag="SDA06_001131"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228420.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12468..12638
                     /locus_tag="SDA06_001132"
     CDS             12468..12638
                     /locus_tag="SDA06_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4228421.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13062..16463)
                     /locus_tag="SDA06_001133"
     CDS             complement(13062..16463)
                     /locus_tag="SDA06_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4228422.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(17135..17403)
                     /locus_tag="SDA06_001134"
                     /pseudo
     CDS             complement(17135..17403)
                     /locus_tag="SDA06_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         5310 a         3674 c         2976 g         5595 t
ORIGIN      
        1 tagccaagaa cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta
       61 caggtatgta aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg
      121 ctttataacg ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa
      181 aacccctttc gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa
      241 caaagtatag ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct
      301 gagtattaga gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
      361 accgagggtc agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct
      421 gagtattagg gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac
      481 tgtaacaaaa gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt
      541 ctacggaaat aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg
      601 cttttcagat ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt
      661 tctctcactt gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct
      721 ttaggcagcc aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact
      781 gctttgacca ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt
      841 gaagtttctg tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca
      901 tcgccatcaa taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg
      961 ctaacctcag ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca
     1021 aacaccccgt cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact
     1081 ggaaaaagat cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga
     1141 ttatcctgag tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt
     1201 tgatacgtaa tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct
     1261 ttatcgcttt tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg
     1321 acttctttgc ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc
     1381 ccccatttca aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta
     1441 tgtcgtaaac gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct
     1501 aaataaccat acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg
     1561 aatttgctat cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa
     1621 agcataatca aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta
     1681 aagcgggcta cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc
     1741 attgtcgcca ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg
     1801 agtttcacta gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct
     1861 tgcagcatgt tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg
     1921 atcagtacat tacttcctag agaatccggt gtataaaata atgtatttag cggataggca
     1981 agtaacataa caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg
     2041 cttgtcaatt ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt
     2101 aacccagtag acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga
     2161 ttggcactaa aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca
     2221 gacatgatct tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc
     2281 acaataataa acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata
     2341 tttattttgt tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa
     2401 aaatacaaaa ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc
     2461 gtgaccgccg tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc
     2521 atataaaaaa tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct
     2581 tgattgcctt gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata
     2641 ctcaaagaac gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa
     2701 ggagaggcga tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat
     2761 gcacggataa ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt
     2821 tgttttgcaa tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata
     2881 ttgtatccca tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac
     2941 cagggaatga tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa
     3001 gcagagcctc gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga
     3061 ttatccatcg gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa
     3121 cagaaactgg caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg
     3181 tttcgttttt ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag
     3241 atatcaatga cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc
     3301 acagttggat tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc
     3361 atcggacatt tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc
     3421 aatcttatga tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact
     3481 gtagcgggaa gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc
     3541 tcctcacatt tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca
     3601 ttgtacacag tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa
     3661 ttttttcggc ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg
     3721 aattattatg tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt
     3781 tcattgttta tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc
     3841 tggattttgt catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa
     3901 gaaattgaat aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt
     3961 actattaaaa gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt
     4021 accagaaaaa gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac
     4081 tttgtttttc tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca
     4141 gggtattcaa tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt
     4201 aacggatatc cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa
     4261 taaattgaaa gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac
     4321 aaaagcaatc ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac
     4381 aacgttagat ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt
     4441 atatcgtatg atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc
     4501 ttcacaagct tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt
     4561 gaatatctcg cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta
     4621 ttgcaagcga caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta
     4681 ttttgattta cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg
     4741 ttcggaagaa agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt
     4801 atcttctcat gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg
     4861 aggattcaac caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc
     4921 cagtcaagat gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt
     4981 gatccaatcc aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa
     5041 aacattgctg aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg
     5101 cagcccagga aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac
     5161 agctgacatc gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc
     5221 ggaagaaatc aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg
     5281 tcctgaagcg attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc
     5341 gggaaaaggc gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga
     5401 ttatgcaatc gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc
     5461 atttctaaga aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca
     5521 ttttaacttt tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt
     5581 atcatagaat ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc
     5641 taacacagca gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga
     5701 aggccacggc atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc
     5761 gagtgtagca gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac
     5821 gattggcatc ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc
     5881 ttttcctaac actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc
     5941 taaagtcgca cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg
     6001 ataatttgcg ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga
     6061 agtgatgata ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat
     6121 caccatgaca ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat
     6181 cattttgaag atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa
     6241 cgcaatgaca aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac
     6301 atctggcatt tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat
     6361 aatcgacata ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc
     6421 catcctaata ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt
     6481 accatcagtc atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc
     6541 ccccgtgagc ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata
     6601 agcaaacaca atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat
     6661 gtacaagtca ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg
     6721 taatggcaaa atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc
     6781 catccccgct gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc
     6841 catcaggtat ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct
     6901 ttttcatatt gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa
     6961 cgatactgta taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac
     7021 tgtcctccat tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac
     7081 actggaatca ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga
     7141 acgttcgttt ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct
     7201 aaaatcagaa tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc
     7261 cattgcatgt tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg
     7321 aaatacatgt ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta
     7381 tcctgcacaa tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt
     7441 gcaacttcca ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt
     7501 tccatttcta tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc
     7561 gcatgaagaa tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct
     7621 ttacgcatcg agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg
     7681 atatcaaaag aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct
     7741 ccttattcga tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag
     7801 atttcctgct gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta
     7861 attccttcat tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat
     7921 tgatcccact gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact
     7981 ggaacagcgg gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca
     8041 cgttgctgga tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc
     8101 ttttgcagca aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa
     8161 aatttttcag acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt
     8221 atcggtctat ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga
     8281 acatcatctt cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc
     8341 gaaagatcaa tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca
     8401 taatagcctt ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc
     8461 tttaatagtt gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc
     8521 gcagctcttt ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct
     8581 tcattgatgg tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca
     8641 aagactttgg gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct
     8701 cggctcaatc gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga
     8761 tcctctacta atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc
     8821 tttgatagtt cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat
     8881 gtcaaataag caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga
     8941 acgatccgct gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat
     9001 tccttgattt caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga
     9061 ttctgtatga taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata
     9121 atgactagct cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc
     9181 aatgaatttc cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt
     9241 tcttcccctt cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga
     9301 atcaatcgct ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc
     9361 tcttctgcca aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata
     9421 tacttttgtc gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact
     9481 atttcttgcc gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag
     9541 ccataacctg gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc
     9601 tgcaagtaat tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg
     9661 gtgacatact ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc
     9721 attatcatcc actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga
     9781 tgcctgtagg gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag
     9841 attctcctaa ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata
     9901 aatcaaaatc actattctta atcatattat cgccttctgt agctgaaact gcatctacga
     9961 gtacctcttc tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg
    10021 acattcctgc cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt
    10081 tataaaccaa gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg
    10141 cggaaaaaat gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat
    10201 agaatttttt caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca
    10261 aatggacaag ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt
    10321 cgtgcagaag aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct
    10381 cttttactga tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa
    10441 atgatggctc aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt
    10501 ttcactgatt taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta
    10561 tcttcacttt ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct
    10621 cttatttttc ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca
    10681 aggatattct caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg
    10741 attgccaaag caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct
    10801 aaagctagac cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata
    10861 aataaaacac atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca
    10921 atcgacatca atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat
    10981 cgctgaaaag ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg
    11041 agcatccaaa ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt
    11101 ttgtctttgt tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaac
    11161 aaaaataata agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac
    11221 gaataaacaa taaagagata tgaaaagccg tacaaagaag cattccccat tttgggatca
    11281 ctagcggttg ccattaatat gctgcttagt aaaataaaat gaatcaacat gcccataact
    11341 gaccatttga tcagctggac atacggtgct actaatagtg agaacatgac gattgctaaa
    11401 cctatgatcg aatgggaaat cttataacca aagtcaacaa aacgaatact catcaacata
    11461 cagaataaaa ccacagcgta tggtttatgc ttcgattcaa ataatgttgt aattataatg
    11521 ataaaacaaa tggcaaacaa tacgatgaat aatgatcgta aaaacaatga acaaatatac
    11581 atgcactttt cttttttaga agctgcttct cgtatttttt gttttaaaat aaacggatct
    11641 agttgtagag actgataaaa atccacctgt tgtcgtcctt tctttttact tttatttttt
    11701 acagtcatct atgacaggtt gtaaatacta tggaacatca tatggcgttg tcattttctt
    11761 gtcaactatt tttcctattc atgtttttat aaaaaaacat atcgttttct tgtatgatta
    11821 aatcaccaca aaataaacaa ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa
    11881 aattctgaga tcaatagaca aaaatttgat gattacagag gtctcttacg aaaccttaca
    11941 gaagaaaaaa acattgatcg ttgctgcagt tctcttccct gctccatgtg tctgtagaaa
    12001 ttgtggttct gctgtggtag atggaaatag gaaattaatg gtggtgaaaa acaagaagaa
    12061 ggaaaccatt gtccactttg aataatataa tcatatgcca atggttatac gcctaaaaaa
    12121 gcagcgctat acctgtaaaa actgtcgaag ccattggaac gctcaaagtt attttatccg
    12181 tccaaggcac tcgatttcaa atcatgtcag acataaaatc acttctttac tgactgaaaa
    12241 agtgtcgtta ttttttattt cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac
    12301 attgaaagag ttgagaagct atttaccaaa gtcttccaaa agaattctgt cttgagtgtt
    12361 gagggttgtg aatttcgttc tcacgtttcc gttgaggata aactgagttt tatttccgcg
    12421 gatggcgaga cagaaaaatt aatcgagaaa atttacctac acaaaaaatt cctcgattaa
    12481 caaactattt ccttagctgc actaatccta aagaaataga attcttagtt acagacatga
    12541 acgccaccta ctttcagctc accaaacatg ttctaccaaa tgcgaaattg gtgattgatc
    12601 gattacttgg tttttccacg cctttcaagg aggcgtaacc tttattcacg agttggttga
    12661 agctttccga gacaaaagac ccgacttgtt tttctcctta ttgacaaaga gccattaaaa
    12721 ctagagaaaa aattacaact gatcgctgat tatttgtaga aagaaaatca aagtattacc
    12781 caataaactt gagtcattag gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg
    12841 tttttgtata ttttttatta actagtatta ctacaaaggt ttatttttca cttctcaacg
    12901 cagcacaaaa ttgtctttac ttatttactg gttgggttca accttctcta ctccgatact
    12961 aatatttgtt tgtatacccc ttctaaaatc ggtagtttcc attcaattga tgggtactag
    13021 ttgattttaa aaactatgct tattcaattt tttattttag ctcatatatt tcgtttcttt
    13081 ctaaatttta tactgatgaa aacaagtagc acacccaata ttgacaatat aaactggcta
    13141 atgatggttt ctccagtttt tggaagatgc ttatttccac tgcttgttgg attgtttcct
    13201 cttatatctg agctatcagc aacttttcta ttctcttttg ttaaaaagat cgccacttct
    13261 tgttcttttt cgatcttgaa agttactggt gtcgcatcca attcataccc cgttggcgct
    13321 tgagtttcta ccagctgata tgctcctggt gctaagccat ctatcgccag ttttccatct
    13381 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
    13441 gctccttgca ggacttctcc gctttggtca tcgatctttg tcaacacgac accgcctggt
    13501 gtaagtcgat tttctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
    13561 tcgattggtg tcgcatccaa ttcatacccc gttggcgctt gggtttctac cagctgatac
    13621 actcctggtg ctaagccgtc aatcgccagt tttccatctg ctcctgtggt tagacctgac
    13681 tgcagaacgg ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttccccg
    13741 ctttggtcat cgatctttgt caacacgaca ccgcctggtg taagtcggtt ttctttcgtc
    13801 aattctacca ccgctgtctg acttctttcg atctcaaact cgatcggtgt cgcatctaat
    13861 tcataccccg ttggcgcttg agtttctacc agctgatatg ctcctggtgc taaaccgtca
    13921 attgccagtt ttccatcttc tcctgtagtt agaccggttt gtagcgtttc tccttctcgg
    13981 ttctgtaact caaaaacagc tccttgcagg atttccccgc tttggtcatc gatctttgtc
    14041 aacacgacac cgcctggtgt aagtcggttt tctttcgtca attctaccac cgctgtctga
    14101 cttctttcga tctcaaactc gattggtgtc gcatctaatt cataccctat tggcgcttga
    14161 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
    14221 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
    14281 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
    14341 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
    14401 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
    14461 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
    14521 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
    14581 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
    14641 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
    14701 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
    14761 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
    14821 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
    14881 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
    14941 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
    15001 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
    15061 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
    15121 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
    15181 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
    15241 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
    15301 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
    15361 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
    15421 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
    15481 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
    15541 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
    15601 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
    15661 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
    15721 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
    15781 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
    15841 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
    15901 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
    15961 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
    16021 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
    16081 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
    16141 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
    16201 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
    16261 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
    16321 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
    16381 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
    16441 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
    16501 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
    16561 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
    16621 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
    16681 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
    16741 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
    16801 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
    16861 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
    16921 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
    16981 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
    17041 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
    17101 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
    17161 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
    17221 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
    17281 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
    17341 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
    17401 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
    17461 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
    17521 ttgattcccc tctaaaatta ctatccataa aaaat
//
LOCUS       DARDHD010000043        17552 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000043 DARDHD010000000
VERSION     DARDHD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.043"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            111..1067
                     /locus_tag="SDA06_001135"
     CDS             111..1067
                     /locus_tag="SDA06_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4228423.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1140..1256
                     /locus_tag="SDA06_001136"
     CDS             1140..1256
                     /locus_tag="SDA06_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG4228424.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1311..2993)
                     /gene="recN"
                     /locus_tag="SDA06_001137"
     CDS             complement(1311..2993)
                     /gene="recN"
                     /locus_tag="SDA06_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG4228425.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3011..3460)
                     /locus_tag="SDA06_001138"
     CDS             complement(3011..3460)
                     /locus_tag="SDA06_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4228426.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3567..4385)
                     /locus_tag="SDA06_001139"
     CDS             complement(3567..4385)
                     /locus_tag="SDA06_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG4228427.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4394..5284)
                     /locus_tag="SDA06_001140"
     CDS             complement(4394..5284)
                     /locus_tag="SDA06_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4228428.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5286..5510)
                     /locus_tag="SDA06_001141"
     CDS             complement(5286..5510)
                     /locus_tag="SDA06_001141"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG4228429.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5515..6852)
                     /locus_tag="SDA06_001142"
     CDS             complement(5515..6852)
                     /locus_tag="SDA06_001142"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG4228430.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6853..7707)
                     /locus_tag="SDA06_001143"
     CDS             complement(6853..7707)
                     /locus_tag="SDA06_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG4228431.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7804..8253)
                     /gene="nusB"
                     /locus_tag="SDA06_001144"
     CDS             complement(7804..8253)
                     /gene="nusB"
                     /locus_tag="SDA06_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG4228432.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8246..8671)
                     /locus_tag="SDA06_001145"
     CDS             complement(8246..8671)
                     /locus_tag="SDA06_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4228433.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8917..9981)
                     /locus_tag="SDA06_001146"
     CDS             complement(8917..9981)
                     /locus_tag="SDA06_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4228434.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10153..10398)
                     /locus_tag="SDA06_001147"
     CDS             complement(10153..10398)
                     /locus_tag="SDA06_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4228435.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10537..10911)
                     /locus_tag="SDA06_001148"
     CDS             complement(10537..10911)
                     /locus_tag="SDA06_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG4228436.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10994..11908)
                     /locus_tag="SDA06_001149"
     CDS             complement(10994..11908)
                     /locus_tag="SDA06_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG4228437.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11929..12732)
                     /locus_tag="SDA06_001150"
     CDS             complement(11929..12732)
                     /locus_tag="SDA06_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG4228438.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12765..13754)
                     /gene="manX"
                     /locus_tag="SDA06_001151"
     CDS             complement(12765..13754)
                     /gene="manX"
                     /locus_tag="SDA06_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG4228439.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13857..14348)
                     /locus_tag="SDA06_001152"
     CDS             complement(13857..14348)
                     /locus_tag="SDA06_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4228440.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14539..17346)
                     /locus_tag="SDA06_001153"
     CDS             complement(14539..17346)
                     /locus_tag="SDA06_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4228441.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         4968 a         3895 c         2905 g         5784 t
ORIGIN      
        1 ttcattttat ttaaatttat gcagttatat tctatatctt acaaacacat tccatgtatt
       61 ccaattccct atccgttata ataaaagaaa caggcaacag gaggaaaatc atgatcaaat
      121 attttacatt agataatgag aaactgcgta ataccggaaa ggaaactgac caaacgatct
      181 ggtattgtgt agaacgtcct aatcaagaag agattcatca gctgacacat caatttgaaa
      241 ttccgtcaga ttacatcact tccatattgg acgatgcaga aaattcccgt acagaagaat
      301 tcaatcaatc aaagttgaca aagccagcgt tattattgtt acaatatcct tttgctaaaa
      361 caagtcctag cggttatttt caagtggata catacccgct ctctattatt ttgacgccca
      421 aaaagaagct tatcacaatt acgaatcatg aaccactgtt tttaaagaaa gtctttagcc
      481 agacctttcc aaaaagtgat atttcgccta atctcaatat cttctttcaa ttcatttggc
      541 aggtcacact ctcttacaac agttatttag ctacgcttca atctcaatgc gatactttgg
      601 aagccaaact tcaagtatcc actgaaaaca aacaacttta tcaaatcatg gatatccaaa
      661 aaagcctcgt ttatttcaga gaagcgactt ctgctaattt agatgcattg aaagaactag
      721 cgcaaaataa acttttccaa gaaaatcatg ctttgaaaaa tcatttacgt gatgtattgg
      781 tggaaacaga gcaggcaatg actacttctc gcattcaatt aaaactagta gaacaaatga
      841 atcagacttt ttcagcaatc gtatcgaata atctgaataa tattatgaaa attttaactt
      901 ctttaacgat tattttaact ataccgacaa tcgtcggcag tatctatgga atgaacatta
      961 agttacctat tgctgaaagg gatgatgcct ttatatggct agttctatta acattattta
     1021 tttgtatcgt aactacctat tatttaaaaa aagggaagtt tttataaaaa agccctcttg
     1081 tggaaatgga aaaaagattg tacacttgaa tcaactaatt aatgacgagg ttgaaaacaa
     1141 tgcgtgaaaa aaaacaaaca agtacttttg ctaaagtaac aaaatttgtc atttggacaa
     1201 tgctgatttt aacgattgga tctgttgtat tgactgctgt aatgagtgtc atgtaaaaat
     1261 aaaatatagc tatcagctgc tgaaaaggac ctgaaccact gcgaggtggc tcaggtcctt
     1321 tttgcgagtt tcaatagttc ttttgcatgt tcgagtgtga gttttgtaat ctcacttcca
     1381 gccagcatac gtgcaatttc ttcttctctt tcttttgttt tcaactctga aacagaagtt
     1441 tgcgtacgtc catcaatcac tgctttcaca atataatatt gatagtcggc gactgcagca
     1501 acttgaggca gatgagtgat gcacaaaact tgagagtatt tcgatatttg agaaatttta
     1561 tctgcgatgg cctgtgcaac tcttccgctc acacctgtat ccacttcatc aaaaataata
     1621 cttgtcacac cttgctcaga agaaaaaata gttttcaaag caagcaacat ccgagacagt
     1681 tcgccaccag aagcgacttt caccaaaggc ttcagtggct ctcctggatt ggtcgtaatg
     1741 taaaattcga tttggtcaaa tccatcatga ttcaattgtc tattttcttc ttttaagaaa
     1801 cgtacctcaa actcagtgtt ttccatatac agacttttca attcatgcag aatggatttt
     1861 tccaattctt ttgcgatttt tttacgcgct tcatgaagat ctgcggcttg ttgctgaagt
     1921 agaagctctt tttgctctag ttcctctttc attttgtcca attgaccttc tgaaaaatca
     1981 gaagaagcta gctcttcggt gatttcgtca tagtaagcca atattgactc aatggattcg
     2041 ccgtatttac gtttcaactg tcggattaat tctaaacgtt gcgtaacttc ttctaaacga
     2101 ctctcatcta actccaataa atcaatctga cgactcatat cgccgattgc atcttgcagc
     2161 aaatagtaag cactttggat attttcataa attgcttcat actccacatc tagatgggcg
     2221 atcccttgca gttctgaaac ggccaagcca acaccatcta cactgttttg ttcattattc
     2281 gtcagtgctc cgtaacctgc agctaatcca tcagcaattt tttgataatt gctaagtttt
     2341 tctctttctt cttttaattt ctcttcttct ccgacttgca aatcagcagc agctatttct
     2401 tcttgctgga aatggagcat atctattctc tgaacatagg atttttcatt ttgctgcact
     2461 tttcgtaccc ttttttccag ctcacgataa gagatatatt cttgctgata actctttttc
     2521 ttctgttgga aagctacatc tccaaaacga tccaataaag caagatgtga ttcaggctgg
     2581 agcagttcct gatgttcgtt ttgtccttgg atatctacta aataactgcc gattcttctc
     2641 agattagcta atgtgataat atgaccattc actcgacaaa cattttttcc agttaaggac
     2701 atatcccgtc gaacaatcag attgtcctca tctgtttcga tccctaattc aaccatcagt
     2761 tcagagaatc cttcttgttt tggtagttca aacaaccctt caagcacaca tttttctgct
     2821 ccttgacgta tataatcgct tgaacctctt cctcctgcta acagacctaa agcatcgata
     2881 ataatggatt tacccgcacc agtctctccg gtcagcgcgg tcattccttc gtgaaaagaa
     2941 agccgcaatt ccggaataat cgcaaagtta gtaatactga tttcttgtag catcacattc
     3001 gctcctcaac ttaaaggtaa tgcaggaagt ctttcttcaa taaaactgcc tggtcttcag
     3061 ttcgtgtaat catcaaaaca ttatcgtcat cattgataat cgaaaataat tctttcttgt
     3121 aacgtttatc aatcaaattt gctgctgccg aagcatttcc cggcaatgtt ttcaaaataa
     3181 caaatttttc catttggtca actgccacga aagcgtcttt taatagtttt tccaatttag
     3241 tatttacatc ttcgtttgtt tcagctggta tgctataacg gtagccgcca tctgaagccg
     3301 gtaccttgat cagtttcagt tcctttatat ctctggaaat cgtagcttga gtaacagata
     3361 ttcctttgct tttcaggatt tcaacaaatt cttcctgctt tcggatatct tgctcactca
     3421 acaatcgagt aatcaaacga tgccgatctt tttttctcat tacgtcttcg ctccttagca
     3481 tattcttctt aatcaatgat agcttatttc cttggtataa taaagaagaa tccgcatact
     3541 tctcacttga ttttcacagt tacaatttat ttatggtcaa actgttcgtg tgcatttgaa
     3601 accacttctg ccatattaat agctgaattg attgttccgc tttcgggtac tttcctcaag
     3661 tgagccaaga actcgatatt tccttctccc cccgtgatcg gagaataact taattccagc
     3721 acatcatatc caccttgtgc cgcaaatgac aaaatgtctt ctaaaacttt ttgatgggtc
     3781 tgagggtcac gaacaattcc gtttttccct acagcttctc tgcctgcttc gaattggggt
     3841 ttgattaaag caatcacttc tccctcttct tttaaaatag catgtaatgg aggcagcatc
     3901 agttttagtg aaataaaaga aacatcgata acagcaactt ctggaattcc taaggtaaag
     3961 tcttcgggtt ttgcataacg aaaattcact ctttccatta cgacgactct aggatcttgt
     4021 cttattttcc aagctagctg attgtagcca acgtctaaag cataactcat ttttgcgcca
     4081 ttttgcaaag ctgcatcagt aaatccgcca gtggatgcac caatatctaa caatgttttt
     4141 ccttcaacag atagatcaaa tgctttgagt gctttttcta attttaatcc ccctcgagaa
     4201 acataaggaa gttttttacc tttgactctt aattcactac tcactggaat tttttctccc
     4261 ggtttatcaa accgctcgtt tttttcgttg taaatcaaac cagccatcac tgaacgtttg
     4321 gcttgttctc ttgtttcaaa taatccttgt ttgaccgcta atacatctac gcgttctttt
     4381 tccattcttt ccctcatttc tccacatcaa attgcttgat catctggcgc atcagcccgc
     4441 catcaaaaga aaaaacgtcc tcttctaatt tgtctatgat cttgttggcg gataacagct
     4501 caatctccaa cgcctcacgt gtcttttcta ggcctaataa acgcgggtaa gtattttttt
     4561 ccatacgttc atctttacca gctgttttcc ctaatgtttt cgttgtacca atcacatcga
     4621 gtagatcatc tcttatctga aaagctaaac ctaaatgttc ggctaatcgt tgaagtagca
     4681 ataagatttc ttcaggttgc ttcgctaata tgccaccagc cagcaaggca taacgaatca
     4741 aatgccctgt ctttctttca tgaataaatg ctaattcttc taaagaaagc tttttcgatt
     4801 cgccctcgat atctgcagct tgcccagcaa ccataccttg tgatcccgca caaacagcta
     4861 attgctgaag cagcagaagc ttaggagaat tacctaagtg tgccatactg attagctgga
     4921 atgctcctgt aagcaaacca tctccggcca atatagctaa tgcttcgccg aatactttat
     4981 gattggtagg ttttcctcta cgaagatcat cattgtccat cgcaggcaaa tcatcgtgga
     5041 tcaaggaata cgtatgcacc atttctaaag cagctgctac ttgatagacg ggaacatcaa
     5101 taggctcatc aaaggaagca acggccgtta aaagtaataa gggacgtatt cttttcccgc
     5161 ctgctttcac tgaataactc atcgcatcat agagtcctct atccgttgta tgctcatcta
     5221 aaaaagacaa gatttctttt tctacagcag gtagatgaat agctgaaaag tcagttaatc
     5281 tcatctcagc tttcctcgtt tccgtcaaaa tcaacttctt catttgattc tgtcatcatt
     5341 ttcgtcaagg tctcttctgc tttagctaat gtatcttggc aatgtttgct taattccatt
     5401 cctcgtttaa acgaatctag agctgattct aatggaacgt ctccttgttc taactgcatg
     5461 acaatttttt ctaattcttg cagtgactct tcaaaagttg gattttccat atttttattc
     5521 tcctttgtct atctcttgta cttgtccttt aacgattcca tcttgataat ggatcgtcag
     5581 caaatcccct tgatgaatct cggtgacact ttttactact tgatcattta acgtcgtata
     5641 gctgtagcct cttcccatga ttttcaatgg actcaacaaa tcaagagatt gaacagcttg
     5701 agcaaaacgc tgctgctgtt gtttcatata ttgttggatt ttctcttcta agcgtttttc
     5761 tagataagca gtttcttggc gagctgcttt aactcgataa agcggagcca cttgttccaa
     5821 ttggtggctt aatgcgatcg tttgtttttc tttcgcatga taaatttgct gtgtagcttg
     5881 atataatcgc tgtttcaact ggtctaattt gatggtctga ccttcataca atctttcagg
     5941 ctgccgaaat acataggaac gtcgagcacg ctcgaaccgt tcttgtttgc gctggatctg
     6001 gcgtacaaac gcctgctgca atctagcttg ccgttcttga atgcgcatca actcttctga
     6061 caaaacagga acagctaatt ctgcagctgc tgttggagtg gcagcccgaa catctgctac
     6121 taaatcagcg atcgtggtat ctgtttcatg tcctacagat gaaatcaccg gcgtattagc
     6181 ttgataaatc gcccgagcaa cccgctcttc attaaacggc cataaatcct caatcgaacc
     6241 acccccacga ccgatgatca ttgtatcgaa attgcccatc tcttctactc tctggatatt
     6301 cctgacaaca ttgtccgctg cttggtttcc ttgtacaact gttgggaaca aaaccaattg
     6361 gacaattgga tatcttcttt tcaccgtcgt gatgatatcg cggataaccg ctccgcttgg
     6421 actggtaagg accgcgattc gctttgggaa acgtggcagc atttttttag gcccttgaaa
     6481 aaggccttcc ttcatcagct tttcttttcg ttcttccaat tcttgatata aagcacctat
     6541 tccatcaggc tccatgtgct ctacatagat ttgataagaa cctcccgctt catataatga
     6601 aatacgccca acgacaagga ctttcattcc ttcttttggc tggaatcgta atttttggaa
     6661 tgctcctttg aacataatag cagatatttt tgcatggtca tcttttaagc tgaaatattg
     6721 atgggcattg gcacgtaaac ggaaattcga tatttcgcct gttaaatata cgcgttctaa
     6781 ataaggatcg gcatcaaatt ttcttttcaa atacttcgtt aatgtagtga cagttaaata
     6841 ttcttgtgcc atctattcac ctgctccaaa agcttcggca gattttacgg tttgatacat
     6901 cagcatcgtg atagtcattg gccctacacc ctttggcaca ggagtgatcc agcctgcaat
     6961 aggctccact tcatcaaatt tcacatcacc gatcagctta ccgttagcat cacgattcat
     7021 ccctacatct atcactacag cacctggttt tacgaattct tttgtgacaa aatgtccacg
     7081 tcctattgct acgactaaaa tatctgcttc ttgtgccagt tcagccagat tttcggtttt
     7141 agaatgagca acagtaacag ttgcatctgc catcaagagt agctgagcca tcggtttacc
     7201 gacgatattg cttcgtccga taactactgc tcgtttaccc gccggatcga tagaataagc
     7261 ttcaaacatt ttcatgatgc cgtatggagt acaaggaatc ttgtccggtt tacccgcaag
     7321 gagtctgcct aaattcatcg gatgaaaacc gtctacgtct ttttcagggc ggatagctaa
     7381 caggatcttt tcttcatcta tatgctttgg caatggtaat tggaccaata taccatgaaa
     7441 actaggatct tgattgtatt tttcaatttc tgcaagtagt tcgtcttcag aaatcgtctc
     7501 aggataacgc tctaccaatg aatggattcc gatttctttt gctacacgtt ctttatttct
     7561 tacatagatt tggctggctg gattttcccc taccaataaa acgactaatc cgggatggat
     7621 accattatct ttcaattttt ggattttttc agcaatctct gcctgcattt tttcagctaa
     7681 ttcttttccg ttaatcaatt ctgccatcta accactccca gatatttttt gtgttttcat
     7741 tctaccatag aattttcccg atttataaca ggtttatgat gattcataga aaaaagctag
     7801 gacttacgcc ccagcttcta attctttcaa tacattggat aaaacaccgt tgacgaattt
     7861 acgtgaacgg tcatcactaa atgtttttgc caactcgatt gcttcattta aagcaacagc
     7921 cgctggtaca tctgtcacat aagtcatttc aaaaatcgcg atacgcaaga tcaccaaatc
     7981 catttttgag atccgattga tgtgccaatt gttttttaaa tgtttttcaa tcactttatc
     8041 caattcatct ttttttgcac agacaccgcc aactagcgta tcaagataaa caggaacaaa
     8101 ctctgattca tcttcgttga tcatgtcgcg gtggtctaat tcgattgcgt tgaaaatcgc
     8161 atcttctttt gttaggtctg cattgaagtc taatggaaat aaagcctgca atgccatttc
     8221 ccgaatctcg tgtcttgata attctttatt cattctcttc ctcttcgtct tcaaaaagtt
     8281 cttcgattgt tggttgttct aatttttcag gaacaacagc tactacatgg atattgactt
     8341 cagataaatc gatatccgtc atgaaaagga cttgctggct gacccgttcc tgcatttcca
     8401 ttgctacttt aggaactgaa acaccatatt ctagataaca gtaaagatct actttcaagc
     8461 catcttcctc gtttgctaag taaacacctt ttccgtgagc tgcacgtcct aataattcag
     8521 tgacattgct ggcaaaagtt ccgcgcattc cgtatacgcc ttctacttta gaagctgcaa
     8581 tcccgataat tacttcgatg acttcaggag cgatcacgat ctctcctaaa tcttggtttg
     8641 cgtttaaaat caaatttttt tcatctgcca tttatttatt cctccctttg cctaagcacg
     8701 agatacatcc gaattctcta tgatgcggca tagtgatcac gtggtaagaa aactgccgcc
     8761 gatcagctgc aaactgctat gaattggaga aaacacccaa ctttattatc tcttacagct
     8821 taaaagttct tctatagtgt atcattaaat cgagtgattt tctataacaa agtctgtgaa
     8881 cactgatttt tacgcttact tcttctattg atgaaactat aagatgatca attcttttgg
     8941 tgaacgagtc aacacacgat ttccatcttc tgtgatcaac aagtcatcct caatacgtac
     9001 tccaccgatt cctggtaagt agatccctgg ttcatcagtg atcacatttc ctggtacaaa
     9061 ttgcttttct gcacgagaag aaacgtttgg tccttcgtgg atctctaaac cgatcccatg
     9121 tcctgtagaa tgtccaaatg cttccccgta accatgtttt gaaatataat ctctagcaac
     9181 tgcatctaat tggacaccag tcattcctgg ttttgcagca tcaataactg ctaattgagc
     9241 atctaagact atttgataaa tttcttttaa tttttcccca ggatcaccaa tagcgaatgt
     9301 acgtgtcata tcggaaacgt acccttcgta atagcaacca aagtccaatg tgatcaagtc
     9361 accttgttcg atgatttttt tgcttgctac accatgcggc atagccgaac gcaatccgct
     9421 agcaacgatc gtatcaaaag aaacacctga agcgccaaga gaacgcatat aaaagtctag
     9481 ttgatttgcg acttcaatct cagtcatgcc cggttggatc attttcagga tgtgatcata
     9541 tgccatatcc gcaatatgac atgctttttc gataatagcg atttcttctt catctttcac
     9601 ttctctgagt tcttcgatca tctgtgaaac aggtactaat tccgcatcga tgatttcttc
     9661 taaaaccgaa tattctaaaa atgagactgt tgtttcctca aaagctaatg agtgcagttc
     9721 ttctttgcgt actaagtttg ctacctcatc aaaaataggt ccgacatttt taataatttc
     9781 aaacccttga gcctgcgctg ccgcctgctc ggtataacga aaatcagtaa taaaaaatgc
     9841 tttttccaaa gtaataacag ctaatcctgt cgtacctgta aagttcgtta agtaacgaag
     9901 attgtacgga ctggtaacca aaaatgaatc caaattttct tcttgcattt ttttgcgcaa
     9961 tttttctaca cgtagcatca taatgaaact ccctctttct ttgcacgctt ttttccatta
    10021 tagcaaactt ttcttcgtac acgacagtta aataaaaatt tccggaaaac ttctttttat
    10081 tttaaataaa taaagccaaa aaggagtcac agtctcgctg caactccttt tagtttacta
    10141 ttttgtaaaa acttatgatc gttttccaaa gaaaaatgag acaactgcta ccaaaatcac
    10201 tgcgcctaaa atagatggaa taagtgccat acctgcagct tttggtcccc attcgccgaa
    10261 aatcgcttgt ccaatacttg aaccgactag tccagcaatg atattcgtta cacagcccat
    10321 cgaatttcct ttactggtga tcgcacctgc aattgctccg atcacggctc caacaatcaa
    10381 tgtccataac caactcataa tataacctcc ttctttgcta atcaatttta gcatatggga
    10441 tttaaagggg acaaataatt ggttttgcta tttcatattt tcttctgcaa agtaaaaaaa
    10501 taaagaaaac ctcctcttag aagttttctt tattttttac ttgcgtttaa acatactacg
    10561 aataccacgt ttcactacat caaagaaact caatgattgt accattctat cttcatctac
    10621 atattctctg atcgcacgaa gcactttttt aggttctttg gaagaaaatg tgtatgtacc
    10681 attttttttc gtttgaatgg catatcgagg aatccatttt ccctttagca tgactgatgc
    10741 tagtacataa tccacttctt cccatggaat ttggatatat ttgcgtctat ctcgcgcatg
    10801 gtaaaactca aaaccattat cacctatcat gatttgcccg taatctgtca gacccgtaaa
    10861 cgaagtagca tcaattacca agtctacttt tgtattgatt gattgaacca ttttctccct
    10921 ccagatattt gtttatgaac agtatactat ataaaaaaag ctcgggacaa cgcccgagct
    10981 atatttttat ggattataaa agtccgatga cgtgtccaac aacaccgact acgaataaac
    11041 caaggatgat aacgattgga gaaacctttt tcttaagcaa ccacatacat aacaatgtta
    11101 atcctaatgc agctaaacca ggaatcaatt ggtctaagtt gtcttgtaat gttgttacct
    11161 tttcaggaga aagggctaag ccattattaa cttgttcaaa tgcactcttg attccttcac
    11221 cacctgaagg caatttatcc cattcgatgt aagcaccttt atctaatttt acagttgata
    11281 caactggtaa gaatttgatt gatacccatc tttgtacaag ggcagctaat acgaacatcc
    11341 ctaaaattga agcacctttt gttacgtctt gtaacaatcc gccagaaagg tcatcagtaa
    11401 ttttagaacc agctttgtag ccaaattctt gtgtatacca catgaatgcc caacgaataa
    11461 tgttccaacc taagaagaag atgattggtc caaggatatt tcctcccata gctaatgaag
    11521 cacctagtgc acctagcatt ggacgtactg taaaccagaa cactgggtca ccaacaccag
    11581 ctaaaggacc catcatacct actttaaccc cttgaatcgc tacatcatca acaggcgcgc
    11641 cattcgcacg ttcttcttcc agagccaatg taacccctaa aattggagaa gcaatgtatg
    11701 ggtgtgtatt aaagaattcc aagtgacgtt ttaatgctgc agaacgttct tcttttgttt
    11761 tgtataattt tttgattgct ggaatcatag aaaatgccca gccaccgttt tgcatacgtt
    11821 cgtagttcca tgaaccttga atgaaggtag agcgccacgc aacagctaaa cgatcttttt
    11881 ttgataattt gattttttct tctgccattt tattttcctc cttcaagatt aatagtcatt
    11941 caaaatgtcg cctaaagggt caccagagtt gcttccgcct ccattatttg atgaaccacc
    12001 cattttagaa aggttcaagt aaataagagc taatgcaaca cctaaagcac ctagagcaat
    12061 caatgttaat tgagaaattg cagctacaac gaaaccaata acgaagaatg gccatacttc
    12121 ttttgtagcc atcatgttga ttaccaaagc gtaacctaca gctacgacca ttcccccacc
    12181 gatagccata ccatctgtta accatgctgg cattgattca aggaatgatt gcacagtttg
    12241 tgctgggata aatagcaacg ctgctgctgg gaccgcgata cgaacccctt gtaaacatac
    12301 agcaatgatg tgccacattt caaccttacg gatgtttcct tcttcagcag ccgcatccat
    12361 caaatgaacg attggaacag cgattgtacg tacgatcatt gttaagaaca accctgcaac
    12421 agccaaagga acggcaatcg cgatcgcaga aggaacacct ttaacacctt gtccacctaa
    12481 aactaaaata attgctgatg ctactgatgc taatgcagca tctggtgcta cagcggcacc
    12541 gatatttgcc caacctaaag cgatcatctg aagcgtaccg ccaagtacga taccagcttc
    12601 gaggttaccg gtaactaaac cgatcaatgt acatgccact agtggttgat gaaattgaaa
    12661 ttcatcaaga ataccttcca taccagctag aaaggcaact aatacgacta aaattattga
    12721 aataatagac atgataagcc tcctataaat tttaatatta tttattacgc ttttgctaat
    12781 tcagcttttg cttttttcaa aatatcatcc atgttatcgc gtgaatcatt aggtacttta
    12841 cgtacatcga atttcacacc tttttgttca agcttctcaa acgcttcaac gtcttcttgt
    12901 cccatagaca atactttact tacaacgact ttaccgaccg aatgcgccat tgacccaaca
    12961 tttaattctt tgatgtctac gccaccttca acagctgtca atacgtcttc aggattttca
    13021 aataaaagca atgctttggt atttccaaaa cgagggtctt ttgctacttc gatcattttg
    13081 ctaataggaa taacatttgc ttttacacct ggaggagcag cttgttcgat tagtctctta
    13141 cgcagatcat cctttgatac tgcatctgaa acaacgataa tacggtttgg ttgcactgct
    13201 tttgtccaag ctgtcgcaac ttgtccatgt aacagacgtg agtcaacacg tgctaataca
    13261 tatttgattt ttccgtctcc gaccacagtt ccttctggaa gtgctccttt aggttgtgcg
    13321 tcttcaactg ctgctttcgg tgcttctgcc ggttctaatt cttccggttt tacttttaca
    13381 ccgtcttttg ctgtttcaat gatgtgtgct gcaatttcat gagcagattc cattgaaaag
    13441 cgtgatgcgt aggcttcaat caacattggt aaattcaaac cgctgacgat tgcccattta
    13501 tctttgtgct cttcgaataa tgtgtttgat tgattgaacg gtgttccgcc ccataaatca
    13561 actaagaata acacttcgtc ttgattatca aaagaagcaa ccgcttcttc caatttggct
    13621 ctcaagtcat ctggaccttc acttggtttc aaaatcacgg ctttgacgtt ttcttgttcg
    13681 ccaaagatca ttgaaccgga ttgtaagatt ccttcagcaa attgtccatg acttgctagg
    13741 ataatcccta ccatatttat acctcctatt tacttttgtt gtaacaaatt caaaaatatt
    13801 aataattgct aaccattaca acagaattat ttgacctttt ccaacaacca ttcttgctac
    13861 acctttttga ttttcgctag tagttccatc aaaaggattt tcctgtcagc aggtactttt
    13921 cgaatctcta actccacttc ttgactgttc aagtaacgaa aggaatcaat atcttgttga
    13981 tttactgaaa caaaattggt aatcattgat ttgccttcag taaaactcat accgccgata
    14041 ttcaacgact tcaactgtac accgcagcgg acaaccgctt caacatcttg gggattggtg
    14101 aataaaagca tcacttttgc tcgtgataat aatccactat gatagatttg aatcattttc
    14161 tgcacggtta ttacatttgc tgtgatcccc ggcggtgcag cttcttttag tagaaacttt
    14221 ctcaatttat cttgagcgac tacatcgctg atcacaatga tacgttcaac acctgtttgc
    14281 ttcgaccaaa ctgttgcgac ttgcccgtgg attaaacgat catctatacg tgccaatcga
    14341 atatccatct gcaaaactcc ttcttcctca ctacttcaca gaaaccccta tcatttctgt
    14401 gtgaacaacc acctcctagc acacactact ataagcaaaa agcgtgccaa cttttttacg
    14461 atacactaat tgaatttaaa ggattcttaa ccaatacaca aaaacgatac actataaaag
    14521 tgtatcgttt ttgtgtattc atggcacagt gtattctttt ttatctaact gattatggac
    14581 tagacgtagc agataataca tttcttctat cggcactttt acttttatct gatcttccaa
    14641 cagaacaact gaggctgcca attggctata aagaggatca ttcgttgtat ttgtcaattc
    14701 gttttcttcc acagtcaatg gttcattccg aataatacgt tcgatcattc ctgctgtgtg
    14761 catcactagg ttgattttga atccatattc ttcatcgccg attcccgtcg tctgagagat
    14821 ttgtgtagaa aattgccaca ttggatcgat taacttacta ccattaataa agatgaagtt
    14881 atcagagata aactcaacac acgtattttt agctgactct tcatctaggg agatgaattc
    14941 agtttcctcc aattctgatt ccatcagcaa ctgatccaaa atgacttcga tattttgatc
    15001 aataaaacgt tctaaaggaa taaatggggc ttggatattc aaatcagtaa tcccagtagt
    15061 agctatgatc tgatattctt tttggataac tggcagttct tctttcactt ccacaatcga
    15121 caaagctaga acttctaatt ctgtttcttg acgcttgttt accgctcgtt cgatcaattc
    15181 tttgattctt tgtgctgtac cttcgccaga tgcgcagata gccaaaacag cttttttctt
    15241 atgaataggt tttgcttcct gaacagattc tacagaagat atttctgcgt aacctcgaaa
    15301 atttttcaat gattcatgca acatatctag atctgtccct aatactgaga cctttcgtac
    15361 agtttccaaa actagagaag ttgttaccat atcaacagta cgtactggaa ttccagtgtc
    15421 tctttgaatt tgcgaattaa acgacgcaag ggaccccata tctactaaga gtatgacccc
    15481 gcttccttca tctacttctt gtacattgcg ttcaattcgg tccaatgctg ttttcggatc
    15541 catatccaaa ggcatgtcta ctgctcgtac gttctctgca tctaacaact gttggacaac
    15601 ttggaccata ctgcttgctg tgctgtttcc gtgagctgca acgactacac cgattctgcc
    15661 gcttgcttga tttgcccgta acgagacgag tagaacggtc aagtaatagt cttcactttc
    15721 tggaatctcc acttgaaaat acgtttcgat ataactacgc acttctttcg caacagcata
    15781 ttcatctgga aaatcaaaag ccatctcacg gatgttatca ttcgtatgcc gttcttctcc
    15841 taagttgatt tttttcaaaa aagaactgat atgcaagctc atagcataaa taaagttttg
    15901 ctgaaactca tatggtaaag atcttttgac cattacataa atctgattgg tcacttctat
    15961 gattttcgga tctacaaatt ctgctaattt gctgtctgca ttaaaagaaa aaccatggtc
    16021 tttatagaaa gacttcagat ggatgttgat atctgtagaa atgaaatgat tgattgcttc
    16081 ttgatccaaa ccatccgact tcagtaaagc tgctttatct ccgatgatat catatagatt
    16141 ataaggtaac tcataggagt ccgattggat tttcatgatc gtatcatttg gtgctacagt
    16201 tatctttggc tccaatattt tagaaagctc tgccattgca ctgcggttgc ttgctaattg
    16261 aatcaatccg ctacgaatcc cttctgtcaa atcatcgatt gtgatcgaga tttcgggtga
    16321 ttgcatatga tttagaaatc ctcgtgcaca tactaactgg atatttgact tcaattgtcc
    16381 tatatttcca taggtcacac tgccgatcaa tgcttttgct acatcttctg tcaaagaaat
    16441 ctttcgctgc gtgcggtttg cttcgtgggc aatcatgatt tttactaaat cgactttctc
    16501 atttgcagga cgtttttcaa aagccggaag tttgatattg attgggatac gccgaacaaa
    16561 agtttctaat agagaagaac ctgggtcttc tgtggtcgca cctacgatcc gtacatttgc
    16621 ttcatgggat ttcgttgttt ccccaagacg actgtaagtt ccatgatcca taaagtaaaa
    16681 gatcatttct tgaccttcag gcggcaaccg gtgaatctca tccaaaaaca gcataccgcc
    16741 atccgcttga tcgataatcc ccattttttc ttcatctgcg cctgtaaaag caccttttgc
    16801 gtacccaaat aaatgactca tcaaaagctc agggttattg gcataatcag cacaattgaa
    16861 aacaatcagt tccgtttcct catctattac atgatgcgct tttgcaaaat gatacatcgc
    16921 gtgtgcaaaa taagtctttc ctgatccggt aggtcctgta atcaaacaat ttaatcctct
    16981 tggcggatac aaaatcgcag cttttgcctg ttccactgaa tttttcatgc tgccgttagc
    17041 tccaatgatt tttgcaaaga tatccttagt atctatttgg agtgtatttt tttcttcaga
    17101 gaccggcgtt tctttgtaac tgactacatg ttttgtaact ggctttacgt gcgtaacgag
    17161 tgtttgatat atgtaacgta ctggtcgtcc gtctgtcttt atcagttttc cttcgcgcac
    17221 tagctgattc agatctttac tgctatttgt tcgctgaatc cctaacactt cagcaatttc
    17281 ctgtgttgtt actccttgat catattctgc ctttgttaaa tgagcggttt tttctttgac
    17341 atacatataa attcggtcaa tgcgttttgc caatggtatc gctccttttc tatagactat
    17401 tgtacttagg cttcaaaaca taatactatg tatttatttc tgtttcgtca actatttacc
    17461 tgattttgtt ttgttgataa cacgcggata gatagaaatg ttcaatttaa aagaacgaaa
    17521 aatcccgata cgatttttat aatggaaaca ag
//
LOCUS       DARDHD010000044        17285 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000044 DARDHD010000000
VERSION     DARDHD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.044"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            21..1448
                     /locus_tag="SDA06_001154"
     CDS             21..1448
                     /locus_tag="SDA06_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228442.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="SDA06_001155"
     CDS             complement(1540..1905)
                     /locus_tag="SDA06_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4228443.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="SDA06_001156"
     CDS             complement(1902..2267)
                     /locus_tag="SDA06_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4228444.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="SDA06_001157"
     CDS             complement(2459..2707)
                     /locus_tag="SDA06_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228445.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="SDA06_001158"
     CDS             2706..2834
                     /locus_tag="SDA06_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG4228446.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="SDA06_001159"
     CDS             2891..4585
                     /locus_tag="SDA06_001159"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG4228447.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="SDA06_001160"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="SDA06_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4685..4858
                     /locus_tag="SDA06_001161"
     CDS             4685..4858
                     /locus_tag="SDA06_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228448.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="SDA06_001162"
     CDS             5236..6369
                     /locus_tag="SDA06_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG4228449.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="SDA06_001163"
     CDS             6400..6720
                     /locus_tag="SDA06_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG4228450.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="SDA06_001164"
     CDS             complement(6749..7213)
                     /locus_tag="SDA06_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228451.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="SDA06_001165"
     CDS             complement(7345..8598)
                     /locus_tag="SDA06_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228452.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="SDA06_001166"
     CDS             complement(8627..8944)
                     /locus_tag="SDA06_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4228453.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="SDA06_001167"
     CDS             complement(8970..9290)
                     /locus_tag="SDA06_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4228454.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="SDA06_001168"
     CDS             complement(9754..11169)
                     /locus_tag="SDA06_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4228455.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="SDA06_001169"
     CDS             complement(11186..12502)
                     /locus_tag="SDA06_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228456.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="SDA06_001170"
     CDS             complement(12652..13350)
                     /locus_tag="SDA06_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG4228457.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14595)
                     /locus_tag="SDA06_001171"
     CDS             complement(13639..14595)
                     /locus_tag="SDA06_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4228458.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SDA06_001172"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SDA06_001172"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4228459.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15716..>17285)
                     /locus_tag="SDA06_001173"
     CDS             complement(15716..>17285)
                     /locus_tag="SDA06_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228460.1"
                     /translation="IFFKNTQSIYTTGDLSFHLSRIKGLSTIFTSPINYETFNYTGYG
                     VNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFL
                     FSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMS
                     LLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWY
                     PMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILF
                     KKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTM
                     IFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTT
                     KGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVIT
                     LPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLT
                     AYLCLFFKIVFHKR"
BASE COUNT         5439 a         3441 c         2931 g         5474 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag attaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgtcactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    15961 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    16021 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    16081 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    16141 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    16201 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    16261 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    16321 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    16381 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    16441 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    16501 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    16561 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    16621 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    16681 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    16741 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    16801 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    16861 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    16921 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    16981 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    17041 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    17101 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    17161 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    17221 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    17281 agatg
//
LOCUS       DARDHD010000045        16863 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000045 DARDHD010000000
VERSION     DARDHD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.045"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(374..3079)
                     /locus_tag="SDA06_001174"
     CDS             complement(374..3079)
                     /locus_tag="SDA06_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG4228461.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3385..4332)
                     /locus_tag="SDA06_001175"
     CDS             complement(3385..4332)
                     /locus_tag="SDA06_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG4228462.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4522..4791
                     /gene="rpsN"
                     /locus_tag="SDA06_001176"
     CDS             4522..4791
                     /gene="rpsN"
                     /locus_tag="SDA06_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4228463.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4967..5329)
                     /locus_tag="SDA06_001177"
     CDS             complement(4967..5329)
                     /locus_tag="SDA06_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4228464.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5340..6461)
                     /locus_tag="SDA06_001178"
     CDS             complement(5340..6461)
                     /locus_tag="SDA06_001178"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG4228465.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6477..6827)
                     /locus_tag="SDA06_001179"
     CDS             complement(6477..6827)
                     /locus_tag="SDA06_001179"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG4228466.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6957..8468)
                     /locus_tag="SDA06_001180"
     CDS             complement(6957..8468)
                     /locus_tag="SDA06_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4228467.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8790..10142)
                     /locus_tag="SDA06_001181"
     CDS             complement(8790..10142)
                     /locus_tag="SDA06_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG4228468.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10295..11371)
                     /locus_tag="SDA06_001182"
     CDS             complement(10295..11371)
                     /locus_tag="SDA06_001182"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG4228469.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11567..13312
                     /locus_tag="SDA06_001183"
     CDS             11567..13312
                     /locus_tag="SDA06_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG4228470.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13592..14029)
                     /locus_tag="SDA06_001184"
     CDS             complement(13592..14029)
                     /locus_tag="SDA06_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG4228471.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14013..15386)
                     /locus_tag="SDA06_001185"
     CDS             complement(14013..15386)
                     /locus_tag="SDA06_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228472.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15708..16196)
                     /locus_tag="SDA06_001186"
     CDS             complement(15708..16196)
                     /locus_tag="SDA06_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228473.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16218..16820)
                     /locus_tag="SDA06_001187"
     CDS             complement(16218..16820)
                     /locus_tag="SDA06_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4228474.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4737 a         3611 c         3066 g         5449 t
ORIGIN      
        1 gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt ggaagagaga
       61 tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag ttgtcacaac
      121 ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac caaattaaaa
      181 acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca gcttttttac
      241 aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa aaaaacggca
      301 aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa cactgatttc
      361 acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg aatttcgtca
      421 ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca acaaacaaac
      481 cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct gcagattccc
      541 gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg aagaatttcc
      601 ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact aagatgataa
      661 tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg atcatcatga
      721 acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg acatctactc
      781 ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc attgtcaaaa
      841 atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg tctttctttc
      901 ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc cacagacata
      961 gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa agcgcattgg
     1021 tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct agtttcaata
     1081 atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg tctgctgctt
     1141 tttggatacc agctttgata tttggccagt cattgttgat caagtccgca gcagcgttca
     1201 cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg gtgatttcat
     1261 tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat ttttgctgaa
     1321 tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg acgatggtat
     1381 ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt gcaggcattt
     1441 ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc gttgagctga
     1501 gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca gtcccaatcg
     1561 tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct gtattgatac
     1621 cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc aattcatcaa
     1681 tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg ttttgcagat
     1741 tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat ttcgtatttc
     1801 cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca ttttgttgtt
     1861 gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt tcatcatcag
     1921 tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct gtcgttgttg
     1981 ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt ggtatcgcat
     2041 ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc tgattttcta
     2101 aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag ccttgttttg
     2161 cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg tcgatctctg
     2221 ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca aggatgattt
     2281 ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt tcacccatct
     2341 catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt tccttgattt
     2401 caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc gtgtccaaat
     2461 tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg ttcgtatcga
     2521 tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt ttgataaatt
     2581 catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc ggtgcaatcg
     2641 cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca ccactagtaa
     2701 aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa tactttccgg
     2761 aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct aattgtttat
     2821 ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct tgaaactctg
     2881 ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca tatggatccc
     2941 ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc gcgatgatca
     3001 gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat aatcgaagcg
     3061 tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa ttactttacc
     3121 atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt tggacatatc
     3181 acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga ttacagcctt
     3241 tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta tctattatac
     3301 gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg tttcagcttt
     3361 tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg
     3421 aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag cagacagatt
     3481 cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc
     3541 ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg
     3601 ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat
     3661 ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg
     3721 atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa
     3781 tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc
     3841 atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta tcattacagt
     3901 atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt
     3961 gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga
     4021 tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc
     4081 tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc
     4141 tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac
     4201 atcataagga ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc
     4261 tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc
     4321 aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg cctttatttt
     4381 gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt
     4441 cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa
     4501 aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag
     4561 caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac
     4621 gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgattta
     4681 atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt
     4741 gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct
     4801 ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg
     4861 ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag
     4921 ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag
     4981 gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg
     5041 gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct
     5101 ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc ccaatgtgag
     5161 tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa
     5221 aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc
     5281 ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct
     5341 tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc
     5401 gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata
     5461 agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata
     5521 catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc
     5581 tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga
     5641 gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatc
     5701 aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag tgcgtgccca
     5761 gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca
     5821 tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc gggaagtttt
     5881 ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct
     5941 gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat
     6001 gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct
     6061 gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct
     6121 gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc
     6181 agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc
     6241 aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct
     6301 gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac tgcaaacaat
     6361 tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta
     6421 tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag
     6481 tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccataca
     6541 ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt taaaatctcg
     6601 acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg
     6661 caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt
     6721 ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga
     6781 gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt
     6841 ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt
     6901 ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt ttatgtttaa
     6961 tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt accgccatct
     7021 ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg ataattaccg
     7081 ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt gtatcctttt
     7141 ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc agctggatct
     7201 tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc ttctaataat
     7261 ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt ttcgatttgt
     7321 tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg acgtaaagtc
     7381 gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc atttggtgta
     7441 acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat acggtgaacg
     7501 tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc tgaaatatct
     7561 aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct cttaaatgaa
     7621 cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc tgcacggtaa
     7681 ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg tccgaagacg
     7741 attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa tttttcgtat
     7801 tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt cgctttgatc
     7861 ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc tggcggcatc
     7921 gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat tttttcgata
     7981 tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa tgtttgaacg
     8041 gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg tgtacccaca
     8101 acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc tccgccgtaa
     8161 acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg
     8221 atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg atctggatcg
     8281 attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt
     8341 ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat aggcgttgct
     8401 tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc tagttcttta
     8461 aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt ttgctacccc
     8521 gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc acgtttttat
     8581 ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag gcattagaaa
     8641 agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca acaagttgtt
     8701 tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat tctaacatag
     8761 tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg ttctaccact
     8821 tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc ttcaagattt
     8881 tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac aggtagttgg
     8941 gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata aaggaaaatc
     9001 atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt taaagactct
     9061 ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt tggcagattt
     9121 ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac atcgctcaaa
     9181 atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat agaaacatgg
     9241 gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat cgtatacgca
     9301 atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt ttctccttct
     9361 actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac atcgccattt
     9421 ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt
     9481 tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat ttccattttg
     9541 gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc gatcattgtg
     9601 attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt ggcgtgatcc
     9661 atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat cgtgtaagga
     9721 agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt ttcagacaag
     9781 acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact
     9841 tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt caaaggatct
     9901 gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct ccaaaatgaa
     9961 gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg cgtaccagca
    10021 agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac attggtaatc
    10081 tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat tgttagattc
    10141 attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc
    10201 atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa tgaacgcggt
    10261 tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca aaaaaagatt
    10321 gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa tcaccgtatt
    10381 tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat cctggcatag
    10441 agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg caccggctca
    10501 atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa
    10561 cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata tattttgctt
    10621 cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc cgaacgtctt
    10681 catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca acgattgctc
    10741 gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg ttttctgctt
    10801 ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga taaagcaaag
    10861 aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc ttaagtactg
    10921 gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc atgattttat
    10981 ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc aatgtctcta
    11041 agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga aaaacgatgg
    11101 ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct tcctctgtct
    11161 gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt tctgttagta
    11221 atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact tggtaataat
    11281 tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca tgctctgcgc
    11341 tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt
    11401 cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact tatttataaa
    11461 aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct agattatttg
    11521 tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa
    11581 tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg tagtgccaaa
    11641 ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac taggccccac
    11701 cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt ctgccttcac
    11761 tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa cggtcaaaaa
    11821 gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt tttcaaaatc
    11881 gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc
    11941 ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg atcttcaact
    12001 atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag ttgatctaaa
    12061 aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct taaaagaaag
    12121 tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc gcagccccaa
    12181 gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa
    12241 cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg gaattcaaaa
    12301 caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga aacaggtgtt
    12361 tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc atgtactaac
    12421 aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg
    12481 ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat ataccatcaa
    12541 ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa aactcacaca
    12601 agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact
    12661 ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga caagttttct
    12721 tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat tcacacatgt
    12781 cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc ttttgctgaa
    12841 agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt ccctcatgga
    12901 agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag aagatacatt
    12961 gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga tccctcaaca
    13021 gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt tggaattgaa
    13081 aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg ttttcggtat
    13141 gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa
    13201 cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat tcgtcattat
    13261 ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt agaaaaaggt
    13321 tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat tgtttctcaa
    13381 atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac cgagaataaa
    13441 aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg aacatggaac
    13501 acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata aaataccttc
    13561 gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt aacgcttgac
    13621 gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt atgccagctt
    13681 ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa
    13741 attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt gaagctaaac
    13801 cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt ttataatccg
    13861 cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat tctttggtcg
    13921 tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct cctaatttta
    13981 tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt ctttttcgcc
    14041 ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac attgatccat
    14101 tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt cagcgtgatc
    14161 aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag cgtcatcaat
    14221 aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac gatccggcga
    14281 tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct gcctgttact
    14341 acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat caagaggacc
    14401 aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt taaacgtttc
    14461 aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg caaatacata
    14521 aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct aaataaaaaa
    14581 aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct tggcaacttc
    14641 cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag caagaaacca
    14701 taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct caaaagaaac
    14761 aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag atttcttttg
    14821 atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta ggccatgacg
    14881 taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg caactcaatc
    14941 gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc atccatattg
    15001 ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac aagccatgct
    15061 gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa tacgcctaat
    15121 attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa cgtgtaacta
    15181 tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa ggcataaaac
    15241 agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa aaataaaaga
    15301 taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt aaaattacgc
    15361 ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt ataactaatc
    15421 tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata tattcttgta
    15481 aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac ttgattactc
    15541 aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat
    15601 aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg taaaccagag
    15661 tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta tgcgagagct
    15721 tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat aagttcttct
    15781 gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc ctgattcact
    15841 cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact
    15901 gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact taaataaatt
    15961 tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa
    16021 aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc
    16081 agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg ccatacttct
    16141 tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac
    16201 ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc
    16261 atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat aaattcccta
    16321 aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc
    16381 agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca
    16441 aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg
    16501 acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca
    16561 aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga
    16621 atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc
    16681 tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga
    16741 atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa
    16801 actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct
    16861 cct
//
LOCUS       DARDHD010000046        16655 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000046 DARDHD010000000
VERSION     DARDHD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.046"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..727)
                     /locus_tag="SDA06_001188"
     CDS             complement(29..727)
                     /locus_tag="SDA06_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG4228475.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="SDA06_001189"
     CDS             complement(720..2072)
                     /locus_tag="SDA06_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG4228476.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="SDA06_001190"
     CDS             complement(2295..3695)
                     /locus_tag="SDA06_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG4228477.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="SDA06_001191"
     CDS             3856..4593
                     /locus_tag="SDA06_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG4228478.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="SDA06_001192"
     CDS             4718..5197
                     /locus_tag="SDA06_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG4228479.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="SDA06_001193"
     CDS             5194..5961
                     /locus_tag="SDA06_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228480.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="SDA06_001194"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="SDA06_001194"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4228481.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="SDA06_001195"
     CDS             complement(6864..7466)
                     /locus_tag="SDA06_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4228482.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="SDA06_001196"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="SDA06_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="SDA06_001197"
     CDS             8147..8731
                     /locus_tag="SDA06_001197"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4228483.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="SDA06_001198"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="SDA06_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG4228484.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="SDA06_001199"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="SDA06_001199"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG4228485.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="SDA06_001200"
     CDS             10632..10811
                     /locus_tag="SDA06_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228486.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="SDA06_001201"
     CDS             10833..11858
                     /locus_tag="SDA06_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG4228487.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="SDA06_001202"
     CDS             12033..13277
                     /locus_tag="SDA06_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG4228488.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="SDA06_001203"
     CDS             13430..14377
                     /locus_tag="SDA06_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4228489.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="SDA06_001204"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="SDA06_001204"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG4228490.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="SDA06_001205"
     CDS             complement(15279..15521)
                     /locus_tag="SDA06_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228491.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="SDA06_001206"
     tRNA            15642..15714
                     /locus_tag="SDA06_001206"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="SDA06_001207"
     CDS             complement(15833..16471)
                     /locus_tag="SDA06_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4228492.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5677 a         2963 c         3340 g         4675 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcg
//
LOCUS       DARDHD010000047        16497 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000047 DARDHD010000000
VERSION     DARDHD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.047"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..330)
                     /locus_tag="SDA06_001208"
     CDS             complement(1..330)
                     /locus_tag="SDA06_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG4228493.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="SDA06_001209"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="SDA06_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG4228494.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="SDA06_001210"
     CDS             1251..2264
                     /locus_tag="SDA06_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4228495.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="SDA06_001211"
     CDS             2264..3541
                     /locus_tag="SDA06_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4228496.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="SDA06_001212"
     CDS             3604..6306
                     /locus_tag="SDA06_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4228497.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="SDA06_001213"
     CDS             6458..6775
                     /locus_tag="SDA06_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4228498.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="SDA06_001214"
     CDS             6805..7125
                     /locus_tag="SDA06_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4228499.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="SDA06_001215"
     CDS             7233..8693
                     /locus_tag="SDA06_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228500.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="SDA06_001216"
     CDS             8761..8982
                     /locus_tag="SDA06_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228501.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="SDA06_001217"
     CDS             9013..9195
                     /locus_tag="SDA06_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG4228502.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="SDA06_001218"
     CDS             9195..9608
                     /locus_tag="SDA06_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG4228503.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="SDA06_001219"
     CDS             9731..10912
                     /locus_tag="SDA06_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228504.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10983..11147)
                     /locus_tag="SDA06_001220"
     CDS             complement(10983..11147)
                     /locus_tag="SDA06_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228505.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11301..11374
                     /locus_tag="SDA06_001221"
     tRNA            11301..11374
                     /locus_tag="SDA06_001221"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11411..11611)
                     /locus_tag="SDA06_001222"
     CDS             complement(11411..11611)
                     /locus_tag="SDA06_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4228506.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(11916..13148)
                     /locus_tag="SDA06_001223"
     CDS             complement(11916..13148)
                     /locus_tag="SDA06_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG4228507.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            13402..13971
                     /locus_tag="SDA06_001224"
     CDS             13402..13971
                     /locus_tag="SDA06_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4228508.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(14149..14589)
                     /locus_tag="SDA06_001225"
     CDS             complement(14149..14589)
                     /locus_tag="SDA06_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG4228509.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            14747..15511
                     /gene="map"
                     /locus_tag="SDA06_001226"
     CDS             14747..15511
                     /gene="map"
                     /locus_tag="SDA06_001226"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG4228510.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            15543..16466
                     /locus_tag="SDA06_001227"
     CDS             15543..16466
                     /locus_tag="SDA06_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG4228511.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         5305 a         2861 c         3411 g         4920 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagagccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtacaaa taacccctgt atcctttgcg
    11401 gtacaggggg ttattttgta taagtagaca ttgtagtttc taaactgcta tgtcctaaac
    11461 gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca tgcgtatgtc
    11521 ttagtccatc aataaacagt cgaagagaaa atagggaaaa attgattact aaagaaaaac
    11581 ttactaggtc gacggtgtag ttgattttca tacttcaata gattcgaatt aaacgatttc
    11641 gattggctta taccagtcct atccaacgaa agatatatcg ttcttaactc tagcggtata
    11701 acttgaagct ttacgtaaag ccaatgggaa tgaactggag atgacttttt attattttta
    11761 gtaatagctg atgggttaat acttacaact ttccttttca aagtaccata taaaaaaacg
    11821 gtggggttgt ttagcgaaat aaaggatcta agccaatagc ttagatcctt tattctatgc
    11881 tttatattat tttttgcatg tgtgcgaaag tatactcaag cccaatcgcc atttcggaaa
    11941 atcggtactg tagagccatc ttcacggatt ccgtcgatat ccatcttgtc agatcctacc
    12001 atgaaatcta cgtgggtttg acttctattc aatccagctt ctgctaattc ttcatcggac
    12061 atttctgtac cgcctttcac actgaaggca taagcagaac ctagtgctaa gtgattcgat
    12121 gcgttttcat caaataatgt attgaaaaag atgatgccag attgagaaat aggtgaaggg
    12181 tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt catctgtatc aagcagtttt
    12241 gctaagacat cttctccttg ttcggcagaa aaatcaacaa cttttccatc tttaaaagta
    12301 aatttcatac ccgagatgat ggtgccagca taactcagtg gctttgtact tgaaatgtaa
    12361 ccatctactc gatgactgtc aggtgctgtg aacacttctt ccgtcggcat atttgccatg
    12421 aatttttctc cacgagcatt atagcttcca gcgccttccc aaagatggtt tttaggcaat
    12481 ccgataatga tatctgtacc gggagcagta tagtgaaggg cagaaaattg ttctcgattc
    12541 aattcttctg ctttttttgc taatttttca tcatgcttct tccatgctaa gacagggtct
    12601 tcttcataga cacgagtcgt tttgaaaatc tgatcccata atgcctcaac ttgtttttct
    12661 tctggcagtt ctgggaagac ttttgctgcc cattgtttac cagccgcagc cacgacagtc
    12721 caactgactt tatttgcttg agttgccttg cgtaaattca ttagtgcttt gccattcgct
    12781 gattgataag acgctactcg atggctatct acaccagcaa atgcatcggg atcggcagaa
    12841 acaacgctga tcctgcttgc tcctttttca agccattcat ccgcttgatc gattttagat
    12901 tgtggtacat tctcaatacg atccgttgca gcatgtaaaa gaaattctct ttggatctgg
    12961 tcgtcagtcc attgaacgat aacctcagcg gctcctagtt tataagcttc ttgtgtgatc
    13021 aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa caactgtgtg tcctttttct
    13081 gtggctacgc cagtttctgc aattaagcgt gcatattttt ttagaagttc attaaaatca
    13141 gataacataa taaaaattcc tcctcaccat tttttctggt aactattagc atgataccat
    13201 ttaattatga ttcaaacaat gaggctttaa ataaaaaaag gaggttattt ttttaaggtt
    13261 aagtttcatg atatatgtta ctacttgcta tttcagcttg tgaagaaaat caaaaactaa
    13321 ttttgcatat caaaaaaaca tgttacaatg aaattaatca atggtggaag cgttataaaa
    13381 aaattaagga gattaaaaaa tatggcaaga aaaaagacga ttactagaga acagattttg
    13441 gcagctgctt atgaagtagc tgccaccgat ggatttacac gatttactgc gagaaacatc
    13501 gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg agtttaaaaa tatggatgac
    13561 ttgagacaag ctctttttga gcagatctat gatcatttac aatatgaagt gttcactgtt
    13621 gagcatacag ggaatacgat tatcgatctt gcttttaatt atatccattt tgcaagaaga
    13681 gagaagaagt tgtatcgttc actgtactta gaagaatatg gtggaggaaa agagatgcag
    13741 aatttctctt atacttactt tgtgaataca gtgaaacgcg atccagctta tgtgaatctt
    13801 tcagaagagc agatcgaatc tttgtataat gggacatgga tcatagctac tgggatcgca
    13861 gctttgatgt cttcaagcat catccatcca actgatgaac agattgaaaa gatgatccaa
    13921 gacagtatag atgctatctt gaaattcaaa gaaccaatcg aaatcgattg aatatactca
    13981 aaagttccac aaatcagtag gtaacaagag atgtgtcaaa ataaggaaaa gttcgttttt
    14041 cttacagcgt atgcattttt aactcccgaa gaactcttcc atgaatgcaa tgaataaaag
    14101 agccatacag attgaataaa tctggatggc tcttttattg caagtatatt aaatttttgc
    14161 gactagttct ttagcgaaag cttctaaatt ttcgatatct tcttcttcag cattcagatc
    14221 cactttaaca ttttcggctc cttttgatgc gcctgttttt gcaaaagcag catcgaaatc
    14281 atccaccgat ttgcagaatt catcgtaaaa agtatcacca gatccgcaga caccgtaaat
    14341 ttttcccaat agatccaatt cttgcaagtc ttcataaaag tctacgatct catcaggcag
    14401 atcgccatca tcgtatgtat aagtagctac tacgcaaata tctgcctcct cgaattcatc
    14461 tgcatctact tgggtacatt cgttgatttc gacttcgata tccagatttt caaatgcttc
    14521 tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg ctagcatata cgatttttgc
    14581 taaagccatg atgtgcctcc tcgtttataa ttatttaaca attatgaatt ataccaaaac
    14641 atcattgaaa agaaaataaa acctaaagac acctggggaa tgtcatttgg ctaaatgtat
    14701 gccgctatgt taaaatgaga gggatttgta aaaaggagac gaaaatatga ttactttaaa
    14761 atcagcacgt gaaattgaaa tgatggcaga atctggagct ttattagcag atgtacataa
    14821 aaatctgcgt gattttataa aaccaggaat cacaagttgg gatattgagg tatttgtcag
    14881 aaactatatc gagagtcatg gcggaattgc tgcgcaaatt ggctttgaag ggtatgaata
    14941 tgcgacttgt atcagtataa atgatgaaat ttgtcatgga ttccctcgta aaaagccttt
    15001 gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat ttgaaaggcg gcatttctga
    15061 ttcttgttgg gcctatgtag ttggagaatc tacacctgag atcgaccgat tgatggaagt
    15121 aacgaagaaa gctttgtaca tcggtattga gcaagcacaa gtagggaatc ggattggtga
    15181 catcggtcat gctatccaaa cttatgtaga aggcgaaaat ctagcgatcg ttcgtgattt
    15241 tattggtcat ggcgttggtc ctacgattca tgaagaacca gcagttcctc attacggaga
    15301 agctggaaaa gggttgcggt tgaaagaagg catggttatc acgatcgaac ctatggttaa
    15361 taccggaact tggaaaatga aaatggatcc taacggctgg actgcgtata caagagacgg
    15421 cggactttct tgccaatatg aacatacatt ggctattact aaagatggtc caagaatttt
    15481 gacatcacaa ggagaagaag gcacttattg atctatccag tcaataggta cgggagaggt
    15541 acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct gatgcgcttt attgagacga
    15601 ctcaaaagcg catcactgat tcagaaatga gtacgacctc tgtcgtcgtt gcttattatt
    15661 tactcttgtc tttatttcca ttgattattg cttttggtaa tattttaccg tttctccaca
    15721 tcgatcaaga gactgtactg acctatttac gagaagtgat tcctgagacc gtttaccagt
    15781 ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc aggaggcttg ttatccgttt
    15841 ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa tgctttgcaa attgcgatga
    15901 ataaagcgta tggcgtagaa aatagaaaaa attttttgat cgtccgtttc ttctcactaa
    15961 ttgtcattgt cttgttcatg attgctatta gtggggtaac tatcgttctt gggttgggac
    16021 agctgatttt ggatgctatt cagccaatcg tacaaatacc cgtgaacttt atcgatcagt
    16081 tccaaacttt gaagtggcca atcacattgg tagctttatt tgtcatcatg ttcttgattt
    16141 atttaattgt ccctaatgct cagttgaagt tgaagacgat cattccgggc acgatcttcg
    16201 ctactgtcgg ctggatgctg ttatcacaag tgtttagtat ctatgctagg tattttgcaa
    16261 cgaggatcag cggttatcag atcataggaa gttttattgt actcatgctt tggcttaatt
    16321 ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc agttgttcaa gaatacgtga
    16381 cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa atggttcaat aaattgaaaa
    16441 ctaaattttc tcataaaaaa aagtagaaac aagagttctt attgtaaatt tatatca
//
LOCUS       DARDHD010000048        16375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000048 DARDHD010000000
VERSION     DARDHD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.048"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SDA06_001228"
     CDS             complement(152..550)
                     /locus_tag="SDA06_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG4228512.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SDA06_001229"
     CDS             complement(547..1320)
                     /locus_tag="SDA06_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4228513.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SDA06_001230"
     CDS             complement(1317..2000)
                     /locus_tag="SDA06_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4228514.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SDA06_001231"
     CDS             complement(2167..3054)
                     /locus_tag="SDA06_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228515.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SDA06_001232"
     CDS             complement(3051..3920)
                     /locus_tag="SDA06_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228516.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SDA06_001233"
     CDS             complement(4057..5343)
                     /locus_tag="SDA06_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228517.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SDA06_001234"
     CDS             5616..6194
                     /locus_tag="SDA06_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4228518.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SDA06_001235"
     CDS             6194..7123
                     /locus_tag="SDA06_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4228519.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SDA06_001236"
     CDS             7120..8856
                     /locus_tag="SDA06_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4228520.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SDA06_001237"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SDA06_001237"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG4228521.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SDA06_001238"
     CDS             complement(9752..10261)
                     /locus_tag="SDA06_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG4228522.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SDA06_001239"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SDA06_001239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG4228523.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SDA06_001240"
     CDS             complement(11562..12653)
                     /locus_tag="SDA06_001240"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG4228524.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SDA06_001241"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SDA06_001241"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG4228525.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SDA06_001242"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SDA06_001242"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG4228526.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SDA06_001243"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SDA06_001243"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG4228527.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4725 a         3621 c         2910 g         5119 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaact
//
LOCUS       DARDHD010000049        16363 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000049 DARDHD010000000
VERSION     DARDHD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.049"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SDA06_001244"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SDA06_001244"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG4228528.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SDA06_001245"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SDA06_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG4228529.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SDA06_001246"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SDA06_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG4228530.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SDA06_001247"
     CDS             2857..3732
                     /locus_tag="SDA06_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG4228531.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SDA06_001248"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SDA06_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG4228532.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SDA06_001249"
     CDS             4629..5063
                     /locus_tag="SDA06_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG4228533.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SDA06_001250"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SDA06_001250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG4228534.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SDA06_001251"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SDA06_001251"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG4228535.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SDA06_001252"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SDA06_001252"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG4228536.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SDA06_001253"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SDA06_001253"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG4228537.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SDA06_001254"
     CDS             13334..14260
                     /locus_tag="SDA06_001254"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG4228538.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SDA06_001255"
     CDS             14442..15335
                     /locus_tag="SDA06_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG4228539.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SDA06_001256"
     CDS             15469..15594
                     /locus_tag="SDA06_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228540.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SDA06_001257"
     CDS             complement(15728..16360)
                     /locus_tag="SDA06_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228541.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5811 a         2788 c         3492 g         4272 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aat
//
LOCUS       DARDHD010000050        16335 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000050 DARDHD010000000
VERSION     DARDHD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.050"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(5..652)
                     /locus_tag="SDA06_001258"
     CDS             complement(5..652)
                     /locus_tag="SDA06_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228542.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(831..1550)
                     /locus_tag="SDA06_001259"
     CDS             complement(831..1550)
                     /locus_tag="SDA06_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4228543.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1681..2832)
                     /locus_tag="SDA06_001260"
     CDS             complement(1681..2832)
                     /locus_tag="SDA06_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG4228544.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3049..3789)
                     /locus_tag="SDA06_001261"
     CDS             complement(3049..3789)
                     /locus_tag="SDA06_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4228545.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3793..4116)
                     /gene="rsfS"
                     /locus_tag="SDA06_001262"
     CDS             complement(3793..4116)
                     /gene="rsfS"
                     /locus_tag="SDA06_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG4228546.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4173..4766)
                     /gene="yqeK"
                     /locus_tag="SDA06_001263"
     CDS             complement(4173..4766)
                     /gene="yqeK"
                     /locus_tag="SDA06_001263"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG4228547.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4750..5400)
                     /locus_tag="SDA06_001264"
     CDS             complement(4750..5400)
                     /locus_tag="SDA06_001264"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG4228548.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5410..5721)
                     /gene="yhbY"
                     /locus_tag="SDA06_001265"
     CDS             complement(5410..5721)
                     /gene="yhbY"
                     /locus_tag="SDA06_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG4228549.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5737..6846)
                     /gene="yqeH"
                     /locus_tag="SDA06_001266"
     CDS             complement(5737..6846)
                     /gene="yqeH"
                     /locus_tag="SDA06_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG4228550.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6843..7370)
                     /locus_tag="SDA06_001267"
     CDS             complement(6843..7370)
                     /locus_tag="SDA06_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG4228551.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7550..8335)
                     /locus_tag="SDA06_001268"
     CDS             complement(7550..8335)
                     /locus_tag="SDA06_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG4228552.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8328..9197)
                     /locus_tag="SDA06_001269"
     CDS             complement(8328..9197)
                     /locus_tag="SDA06_001269"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG4228553.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9198..10577)
                     /locus_tag="SDA06_001270"
     CDS             complement(9198..10577)
                     /locus_tag="SDA06_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG4228554.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10585..11004)
                     /gene="fabZ"
                     /locus_tag="SDA06_001271"
     CDS             complement(10585..11004)
                     /gene="fabZ"
                     /locus_tag="SDA06_001271"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4228555.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11029..11505)
                     /gene="accB"
                     /locus_tag="SDA06_001272"
     CDS             complement(11029..11505)
                     /gene="accB"
                     /locus_tag="SDA06_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG4228556.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11508..12743)
                     /gene="fabF"
                     /locus_tag="SDA06_001273"
     CDS             complement(11508..12743)
                     /gene="fabF"
                     /locus_tag="SDA06_001273"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG4228557.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12762..13499)
                     /gene="fabG"
                     /locus_tag="SDA06_001274"
     CDS             complement(12762..13499)
                     /gene="fabG"
                     /locus_tag="SDA06_001274"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG4228558.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13511..14428)
                     /gene="fabD"
                     /locus_tag="SDA06_001275"
     CDS             complement(13511..14428)
                     /gene="fabD"
                     /locus_tag="SDA06_001275"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4228559.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14535..14759)
                     /locus_tag="SDA06_001276"
     CDS             complement(14535..14759)
                     /locus_tag="SDA06_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4228560.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14812..15774)
                     /locus_tag="SDA06_001277"
     CDS             complement(14812..15774)
                     /locus_tag="SDA06_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG4228561.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15784..16224)
                     /locus_tag="SDA06_001278"
     CDS             complement(15784..16224)
                     /locus_tag="SDA06_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4228562.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4307 a         3722 c         2791 g         5515 t
ORIGIN      
        1 tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa
       61 taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac
      121 ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa
      181 ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg
      241 ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga
      301 taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca
      361 tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa
      421 tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac
      481 ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg
      541 cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc
      601 ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc
      661 ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct catcatacca
      721 caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga
      781 ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct
      841 aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt
      901 ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg
      961 atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg
     1021 aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca
     1081 tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca
     1141 aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca
     1201 tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta
     1261 tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt
     1321 tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta
     1381 taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca
     1441 cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta
     1501 gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt
     1561 cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat
     1621 cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat
     1681 ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata
     1741 gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga
     1801 ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag
     1861 tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa
     1921 gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga
     1981 cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc
     2041 ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg
     2101 caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt
     2161 ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt
     2221 tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg
     2281 gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc
     2341 caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac
     2401 ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc
     2461 tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc
     2521 ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc
     2581 tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc
     2641 cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc
     2701 tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg
     2761 tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc
     2821 gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta
     2881 aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat
     2941 acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt
     3001 tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta
     3061 tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa
     3121 tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata
     3181 ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc
     3241 aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca
     3301 caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac
     3361 ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac
     3421 ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa
     3481 acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa
     3541 ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga
     3601 aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt
     3661 accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc
     3721 taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc
     3781 ataagccata tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag
     3841 tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc
     3901 aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt
     3961 gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt
     4021 tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat
     4081 aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact
     4141 atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat
     4201 gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt
     4261 tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt
     4321 accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg
     4381 agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg
     4441 gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta
     4501 ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca
     4561 tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa
     4621 gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag
     4681 cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta
     4741 tattcattac tatagtccat attcatacaa cccttcattt tgaatataat caattacttt
     4801 atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga
     4861 actgatgtcg atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta
     4921 tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca
     4981 ttttggcaaa tattctacca tatccccacc gataataaag taataatctg tatccggatt
     5041 gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc
     5101 aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg
     5161 atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa
     5221 aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag
     5281 gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg
     5341 aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat
     5401 ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga
     5461 aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt
     5521 ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt
     5581 gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc
     5641 accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt
     5701 ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa
     5761 tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca
     5821 gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag
     5881 ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt
     5941 ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac
     6001 ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa
     6061 gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc
     6121 aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc
     6181 cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt
     6241 tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg
     6301 attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc
     6361 gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt
     6421 taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt
     6481 taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac
     6541 tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat
     6601 atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa
     6661 gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg
     6721 ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc
     6781 taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc
     6841 tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata
     6901 attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt
     6961 tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga
     7021 tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc
     7081 cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct
     7141 cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc
     7201 atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac
     7261 gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta
     7321 atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct
     7381 catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat
     7441 cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa
     7501 tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc
     7561 ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca
     7621 atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc
     7681 cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag
     7741 taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg
     7801 caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg
     7861 ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg
     7921 gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct
     7981 cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat
     8041 tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag
     8101 aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg
     8161 ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata
     8221 aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg
     8281 tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct
     8341 atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg
     8401 cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct
     8461 tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag
     8521 ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg
     8581 gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca
     8641 gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca
     8701 cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg
     8761 gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact
     8821 ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt
     8881 cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct
     8941 ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga
     9001 cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg
     9061 atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga
     9121 acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt
     9181 ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa
     9241 aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc
     9301 aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct
     9361 cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc
     9421 attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt
     9481 cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg
     9541 aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct
     9601 tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg
     9661 attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg
     9721 aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg
     9781 actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc
     9841 actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc
     9901 tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga
     9961 gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct
    10021 tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct
    10081 ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt
    10141 tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt
    10201 gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt
    10261 ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca
    10321 gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt
    10381 acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca
    10441 tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg
    10501 atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc
    10561 aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac
    10621 ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa
    10681 tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt
    10741 gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac
    10801 tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg
    10861 aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc
    10921 tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat
    10981 ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg
    11041 aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt
    11101 gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca
    11161 gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc
    11221 tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc
    11281 cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac
    11341 tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt
    11401 atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga
    11461 ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag
    11521 cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa
    11581 gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt
    11641 ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag
    11701 gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg
    11761 taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca
    11821 ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca
    11881 gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct
    11941 gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca
    12001 ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc
    12061 acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct
    12121 gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt
    12181 cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt
    12241 gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca
    12301 cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa
    12361 actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca
    12421 ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa
    12481 ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt
    12541 cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct
    12601 aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc
    12661 tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag
    12721 ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat
    12781 ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct
    12841 gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt
    12901 tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg
    12961 gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact
    13021 acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca
    13081 ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc
    13141 gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc
    13201 attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat
    13261 ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca
    13321 ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga
    13381 atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc
    13441 gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata
    13501 acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca
    13561 gaactggtat tgttttatcg atttttttca taaatccgct taatactttt ccggggccga
    13621 cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa
    13681 caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg
    13741 ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct
    13801 tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta
    13861 gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt
    13921 tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc
    13981 cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg
    14041 caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta
    14101 ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc
    14161 ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat
    14221 acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat
    14281 tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg
    14341 tctgctttac aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt
    14401 ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt
    14461 gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa
    14521 cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt
    14581 ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt
    14641 tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt
    14701 tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata
    14761 ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc
    14821 catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg
    14881 ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc
    14941 agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac
    15001 ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac
    15061 tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa
    15121 aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg
    15181 atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa
    15241 tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc
    15301 gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc
    15361 accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc
    15421 ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa
    15481 tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac
    15541 taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac
    15601 taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt
    15661 ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat
    15721 aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata
    15781 tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg
    15841 ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg
    15901 atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc
    15961 atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt
    16021 caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta
    16081 catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt
    16141 tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt
    16201 tactgttttt aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc
    16261 ttttgatgtt caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt
    16321 ttactatatg agctt
//
LOCUS       DARDHD010000051        16204 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000051 DARDHD010000000
VERSION     DARDHD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.051"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..1208
                     /locus_tag="SDA06_001279"
     CDS             39..1208
                     /locus_tag="SDA06_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4228563.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1782..2351
                     /locus_tag="SDA06_001280"
     CDS             1782..2351
                     /locus_tag="SDA06_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG4228564.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2338..3198
                     /locus_tag="SDA06_001281"
     CDS             2338..3198
                     /locus_tag="SDA06_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4228565.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3388..4092
                     /locus_tag="SDA06_001282"
     CDS             3388..4092
                     /locus_tag="SDA06_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228566.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4097..5932
                     /gene="walK"
                     /locus_tag="SDA06_001283"
     CDS             4097..5932
                     /gene="walK"
                     /locus_tag="SDA06_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG4228567.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5929..7245
                     /locus_tag="SDA06_001284"
     CDS             5929..7245
                     /locus_tag="SDA06_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228568.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7246..8115
                     /locus_tag="SDA06_001285"
     CDS             7246..8115
                     /locus_tag="SDA06_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG4228569.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8170..8979
                     /locus_tag="SDA06_001286"
     CDS             8170..8979
                     /locus_tag="SDA06_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4228570.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9082..9711)
                     /locus_tag="SDA06_001287"
     CDS             complement(9082..9711)
                     /locus_tag="SDA06_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG4228571.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9881..11173
                     /locus_tag="SDA06_001288"
     CDS             9881..11173
                     /locus_tag="SDA06_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4228572.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11249..11911)
                     /locus_tag="SDA06_001289"
     CDS             complement(11249..11911)
                     /locus_tag="SDA06_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4228573.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12136..12420
                     /gene="groES"
                     /locus_tag="SDA06_001290"
     CDS             12136..12420
                     /gene="groES"
                     /locus_tag="SDA06_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG4228574.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12471..14096
                     /gene="groL"
                     /locus_tag="SDA06_001291"
     CDS             12471..14096
                     /gene="groL"
                     /locus_tag="SDA06_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG4228575.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14185..15333)
                     /locus_tag="SDA06_001292"
     CDS             complement(14185..15333)
                     /locus_tag="SDA06_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4228576.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15435..16019)
                     /locus_tag="SDA06_001293"
     CDS             complement(15435..16019)
                     /locus_tag="SDA06_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4228577.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         5346 a         2730 c         3331 g         4797 t
ORIGIN      
        1 atttttgccg atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa
       61 ttgaaaaaag aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta
      121 gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa
      181 ttagagatga atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta
      241 atccaaattt attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag
      301 tagcatttaa cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt
      361 tttatcctaa aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc
      421 aatatatgtt aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac
      481 gcaaagctct tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag
      541 taagaaagcc aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga
      601 aattacttga cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt
      661 atggattgcg aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca
      721 aaaaaatcat gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa
      781 agaaagatca aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg
      841 aacgagcatt agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg
      901 attatatttt agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc
      961 ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc
     1021 gctttcatga cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga
     1081 aatccattca agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc
     1141 atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta
     1201 aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt
     1261 ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat
     1321 ttataaagct ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc
     1381 taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt
     1441 aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca
     1501 ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg
     1561 caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc
     1621 gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc
     1681 atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag
     1741 tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc
     1801 aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg gaagaactcc
     1861 ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg
     1921 tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac
     1981 taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac
     2041 ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt
     2101 tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag
     2161 cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc
     2221 aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac
     2281 ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg
     2341 aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgttccaga agaattgata
     2401 aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca
     2461 gataaaattg acattacccc tgaagagatc tatcaacgtt tgccagagga aatccctggt
     2521 acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt
     2581 tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt
     2641 cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt
     2701 ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa
     2761 tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat
     2821 gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta
     2881 ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca
     2941 gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa
     3001 aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa
     3061 gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt
     3121 ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta
     3181 caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct
     3241 tttttattga agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct
     3301 cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg
     3361 acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag
     3421 ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgacgt ttatactgca
     3481 tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt
     3541 gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat
     3601 gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta
     3661 gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt
     3721 gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct
     3781 gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga
     3841 ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac
     3901 cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt
     3961 ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat
     4021 ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca
     4081 gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat
     4141 cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat
     4201 acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga
     4261 atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga
     4321 ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat
     4381 acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc
     4441 tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc
     4501 tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg
     4561 cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat
     4621 caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat
     4681 cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc
     4741 cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact
     4801 tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac
     4861 aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat
     4921 tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga
     4981 tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga
     5041 atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag
     5101 tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc
     5161 aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga
     5221 acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag
     5281 tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc
     5341 aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct
     5401 cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt
     5461 caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa
     5521 aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga
     5581 taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg
     5641 gggaacgatt acttgtcgct tattagaaac gcataataac gtgattttaa gtatcaccga
     5701 tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt
     5761 agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga
     5821 agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac
     5881 tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct
     5941 gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat
     6001 gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa
     6061 atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca
     6121 tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa
     6181 ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca
     6241 cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt
     6301 ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa
     6361 gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga
     6421 tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat
     6481 atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg
     6541 acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta tagttatatc
     6601 ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg
     6661 aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc
     6721 cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct
     6781 agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc
     6841 agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg
     6901 gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt
     6961 tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac
     7021 gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca
     7081 gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg
     7141 aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca
     7201 gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa
     7261 atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat
     7321 catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa
     7381 caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa
     7441 ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag
     7501 gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa
     7561 tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc
     7621 ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg
     7681 aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa
     7741 ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat
     7801 gattatagca tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa
     7861 aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata
     7921 ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg
     7981 gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc
     8041 caaattgaga gcgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa
     8101 gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt
     8161 aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt
     8221 tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt
     8281 agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat
     8341 ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt
     8401 aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt
     8461 ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc
     8521 gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt
     8581 ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat
     8641 atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag
     8701 atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga
     8761 catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa
     8821 cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca
     8881 atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat
     8941 acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata
     9001 taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt atttttttag
     9061 catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt
     9121 ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc
     9181 gctagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc
     9241 atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt
     9301 caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg
     9361 tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc
     9421 aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt
     9481 ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga
     9541 tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg
     9601 caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc
     9661 taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct
     9721 ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt
     9781 tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag
     9841 aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg
     9901 tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa
     9961 tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca
    10021 ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac
    10081 acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata
    10141 cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc
    10201 ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga
    10261 agggatacta ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt
    10321 aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt
    10381 tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt
    10441 tcaacatttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat
    10501 tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt
    10561 atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca
    10621 agcgaattca ttagcaaatg cagaaatctt agcctcagga caagagttga cggtggcaaa
    10681 tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg
    10741 attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga
    10801 attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc
    10861 cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc
    10921 aggattgatt acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca
    10981 agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc
    11041 gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa
    11101 aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa
    11161 gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct
    11221 aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa
    11281 caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag
    11341 ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat
    11401 cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat
    11461 atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag
    11521 caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg
    11581 tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt
    11641 gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag
    11701 ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta
    11761 ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt
    11821 ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa
    11881 tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc
    11941 atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt
    12001 cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt
    12061 attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta
    12121 tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa
    12181 gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa
    12241 actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct
    12301 gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa
    12361 tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa
    12421 atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag
    12481 aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag
    12541 atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg
    12601 gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc
    12661 attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg
    12721 caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa
    12781 aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa
    12841 aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg
    12901 cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa
    12961 tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag
    13021 aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa
    13081 cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa
    13141 aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc
    13201 cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca
    13261 aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta
    13321 aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact gacgatctag
    13381 gtttagaatt gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag
    13441 ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg
    13501 tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac
    13561 aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg
    13621 aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg
    13681 cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag
    13741 tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt
    13801 tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg
    13861 ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa
    13921 acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg
    13981 ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac
    14041 cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg atgtagtaat
    14101 taagaaagaa ccctatttta acagggttct ttcttctttt tttgttttat tttaagcaaa
    14161 aggggcaaag aaggggcaaa aatatcaaag gctatttagt ttatcaatta cttgttgttt
    14221 ggctcttttt gttacatggt tataaataga cagagttgtg tttgcatcag aatgccctac
    14281 acgctccata atggctttaa gaggtacgcc taattcagat aatagtgaaa cgtggctatg
    14341 tctgaatata tgtgaggata gacttttttc taattccaac tcttcctcta ctttatggag
    14401 tatagcgtta aatgagtgca gcgcaagcgg agtgccactt gtagatataa atatatattg
    14461 atcgggatct gtgggtctac ctgcaagaat attgtcagct atcacacttt caatcaattc
    14521 ttttgcacga ttgggtaatt gcacttcacg ttgcgaataa gtattttttg gagttgtttt
    14581 tatagcatta tccattttca cagatgtgta atctaaggtc ccattaatgg aaattttccc
    14641 atcctcatag tccttcattt gcaaagctag caattcccca tatctcaaac cagttaaata
    14701 tagaaactca gctattatgc cgtgtagttt tctgcgagga ttggagtata gctgtttcag
    14761 tatttgatca atttcttctt tctccagata ttttttattc atagaaagcc ttcttttttc
    14821 ttcttccact tttttaggat ggattttaac tgctagcgca gggtttcttt gaatgtattt
    14881 tctatcgatt gcatagttta gcataacaga tagagttgtt tttgtttgtt ttgtgtagtt
    14941 cagtgagagg tcaccaaacg tatacatatc ttcaattatc ttattaatga gtgtctcatc
    15001 aatgtttcta acgatcgtat catcgcttat gtgcttagaa acatgtttca tcatcattgg
    15061 aaccttcaaa tagctagtac gtttaacatg ctgcttataa tattcatacc attctttata
    15121 cagctcacca aaagtgatat ctgatttatt gtaatcttcg agtgcttctt taattttttt
    15181 atctagaatt ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga
    15241 tttttttcgt gttttttctg tataaggatc tacatatctt tcaataaact tgaattggcc
    15301 atctttggtc tgttcaaccc acacttttaa catctcctat catttgctat aataggcata
    15361 acaaatagac ctatataggt ttgttttcta aaagcacgct cttactctgg acggtggggc
    15421 gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa
    15481 ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc
    15541 atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt
    15601 ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca
    15661 ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt
    15721 ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg
    15781 tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa
    15841 gatttagtta ctttttcgcc accattatca cttgctttat tactatctga tccaccattt
    15901 aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca tacttttttg
    15961 taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt
    16021 taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc
    16081 gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
    16141 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
    16201 gcat
//
LOCUS       DARDHD010000052        16050 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000052 DARDHD010000000
VERSION     DARDHD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.052"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SDA06_001294"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SDA06_001294"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG4228578.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SDA06_001295"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SDA06_001295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG4228579.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SDA06_001296"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SDA06_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG4228580.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="SDA06_001297"
     CDS             complement(4503..5270)
                     /locus_tag="SDA06_001297"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG4228581.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SDA06_001298"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SDA06_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG4228582.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="SDA06_001299"
     CDS             6383..6781
                     /locus_tag="SDA06_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228583.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7060..7560
                     /locus_tag="SDA06_001300"
     CDS             7060..7560
                     /locus_tag="SDA06_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4228584.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SDA06_001301"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SDA06_001301"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4228585.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="SDA06_001302"
     CDS             complement(8346..8573)
                     /locus_tag="SDA06_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG4228586.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SDA06_001303"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SDA06_001303"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4228587.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="SDA06_001304"
     CDS             complement(9108..9821)
                     /locus_tag="SDA06_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG4228588.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="SDA06_001305"
     CDS             complement(9824..10720)
                     /locus_tag="SDA06_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4228589.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="SDA06_001306"
     CDS             complement(10878..11720)
                     /locus_tag="SDA06_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4228590.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="SDA06_001307"
     CDS             11852..12505
                     /locus_tag="SDA06_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG4228591.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="SDA06_001308"
     CDS             complement(12568..13095)
                     /locus_tag="SDA06_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4228592.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="SDA06_001309"
     CDS             complement(13115..14062)
                     /locus_tag="SDA06_001309"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG4228593.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15960)
                     /locus_tag="SDA06_001310"
     CDS             complement(14074..15960)
                     /locus_tag="SDA06_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4228594.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         4227 a         3323 c         2809 g         5691 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa
//
LOCUS       DARDHD010000053        15986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000053 DARDHD010000000
VERSION     DARDHD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.053"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            216..884
                     /locus_tag="SDA06_001311"
     CDS             216..884
                     /locus_tag="SDA06_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG4228595.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1084..1470)
                     /locus_tag="SDA06_001312"
     CDS             complement(1084..1470)
                     /locus_tag="SDA06_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228596.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1442..2824)
                     /locus_tag="SDA06_001313"
     CDS             complement(1442..2824)
                     /locus_tag="SDA06_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG4228597.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2980..4167)
                     /locus_tag="SDA06_001314"
     CDS             complement(2980..4167)
                     /locus_tag="SDA06_001314"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG4228598.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4174..5205)
                     /locus_tag="SDA06_001315"
     CDS             complement(4174..5205)
                     /locus_tag="SDA06_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4228599.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5221..6675)
                     /locus_tag="SDA06_001316"
     CDS             complement(5221..6675)
                     /locus_tag="SDA06_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228600.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6850..7755
                     /locus_tag="SDA06_001317"
     CDS             6850..7755
                     /locus_tag="SDA06_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4228601.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7846..8523
                     /locus_tag="SDA06_001318"
     CDS             7846..8523
                     /locus_tag="SDA06_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4228602.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8520..8690
                     /locus_tag="SDA06_001319"
     CDS             8520..8690
                     /locus_tag="SDA06_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228603.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8804..9700
                     /locus_tag="SDA06_001320"
     CDS             8804..9700
                     /locus_tag="SDA06_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4228604.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9755..11125)
                     /locus_tag="SDA06_001321"
     CDS             complement(9755..11125)
                     /locus_tag="SDA06_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4228605.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11363..12109
                     /locus_tag="SDA06_001322"
     CDS             11363..12109
                     /locus_tag="SDA06_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4228606.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12106..12945
                     /locus_tag="SDA06_001323"
     CDS             12106..12945
                     /locus_tag="SDA06_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4228607.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12946..13896
                     /locus_tag="SDA06_001324"
     CDS             12946..13896
                     /locus_tag="SDA06_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4228608.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14037..14789
                     /locus_tag="SDA06_001325"
     CDS             14037..14789
                     /locus_tag="SDA06_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228609.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14808..15959
                     /gene="queG"
                     /locus_tag="SDA06_001326"
     CDS             14808..15959
                     /gene="queG"
                     /locus_tag="SDA06_001326"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG4228610.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5190 a         2999 c         3220 g         4577 t
ORIGIN      
        1 cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa aaaaggagtg
       61 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca cagtcctttt
      121 tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga gaaagcatat
      181 ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt acaatgaaac
      241 tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag aaatccatgt
      301 cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc ttagaagaat
      361 caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg ctttgggcag
      421 taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat cgttgggtca
      481 aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag ttgacaaata
      541 accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc ttttctcttg
      601 aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg aaaaaatcag
      661 atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag cataaagtgc
      721 ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa gaaggctatg
      781 cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc tacatactaa
      841 ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac agaggttgtg
      901 aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc tctgatatct
      961 aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc acaaaaattt
     1021 aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt ctgtcacaac
     1081 ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta cgtcttccca
     1141 caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa tgaagtgccc
     1201 ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa tgattactgc
     1261 tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg tcatttgttg
     1321 tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt tgactagttc
     1381 ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat actcgttata
     1441 gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca ctcataccac
     1501 ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc aagagactat
     1561 gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca acaaaatcaa
     1621 ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt aaacctgtcg
     1681 cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag tggtaatgct
     1741 ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc gcttcgattt
     1801 tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa gagtatccta
     1861 cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg ccgacttggt
     1921 cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct ctatctagga
     1981 tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa aaacttcgat
     2041 cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg cttttcaagt
     2101 gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt ttcagacgtt
     2161 ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc cataactttt
     2221 ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt gagccattgt
     2281 ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg tagattccgg
     2341 tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt gttttaccaa
     2401 agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag acatgatggt
     2461 tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt tccatataat
     2521 aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga agatctctgg
     2581 cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa gccaagatta
     2641 atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact aatttagttg
     2701 taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat ttgcagtcaa
     2761 tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt tgattcgccc
     2821 acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt gtcaataaaa
     2881 aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga gaggttattt
     2941 ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa cagcttctgg
     3001 atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag catagccgac
     3061 aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt cttctttgac
     3121 aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta agacaccttc
     3181 ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt cataatcata
     3241 gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt tcccagcttc
     3301 taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc gagctctgcg
     3361 tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt catctaagtc
     3421 tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt cgcattcgct
     3481 acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat tcatgatgac
     3541 gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat ttgtatctaa
     3601 taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga tacctgaatt
     3661 gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca agcgatctgt
     3721 ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg aagcgctact
     3781 ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc catgatcgat
     3841 tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg cccagtttgc
     3901 ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat cgtcaaagtt
     3961 tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt gcgttccata
     4021 agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac caatcaaatt
     4081 gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt cggcaaattt
     4141 tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt tgattttgaa
     4201 ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa atgctgagaa
     4261 gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat gtgccagttt
     4321 tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa ccacactaga
     4381 agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc gagcggcttc
     4441 tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa caagattttt
     4501 ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa aatcgaaagc
     4561 acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca aaggagcata
     4621 agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat ctcccgtcaa
     4681 gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg tgccgcgaga
     4741 ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat ccattgtacc
     4801 tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag cttcattttc
     4861 gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac caccatgcag
     4921 attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga gagaaaagcg
     4981 gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg gatctttttc
     5041 gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg attttaaact
     5101 gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt tctccaaggt
     5161 tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca agatcctttc
     5221 ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag ctggagccaa
     5281 tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg taatcttcca
     5341 agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg cataagcttt
     5401 gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag tcatcccaga
     5461 agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt tatctagtaa
     5521 agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt gaccatattc
     5581 aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag ggaagtagaa
     5641 caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa agaaaaagta
     5701 agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga tgacgcgtgt
     5761 aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta gaccaacgat
     5821 ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata cggcagttgt
     5881 tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca actgatcatt
     5941 ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct gacggatcgc
     6001 agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg aaataacagc
     6061 agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat gagtaacaag
     6121 ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg cacctagaga
     6181 agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg tactcaaagc
     6241 tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca ataagatgaa
     6301 aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc caaagaaatt
     6361 ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta accatggttt
     6421 gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag gatcaaaaat
     6481 aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca tttttgcatt
     6541 cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa aataagtgct
     6601 taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca cacgttggat
     6661 cattttgctt ttcataatct gtattcctct cctgttcaat atataatact aagtaaaccg
     6721 gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat cgcttccttt
     6781 ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa ggagtaagga
     6841 gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt agaaaaagaa
     6901 ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt tggtcctgct
     6961 attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta tcatgtgaaa
     7021 aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg agactcgacg
     7081 ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt tggtgggagt
     7141 gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac gatggtctct
     7201 tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac ggaagaaacc
     7261 ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt ccttttaaaa
     7321 gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc tgttgaaaca
     7381 gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat cgcccagcat
     7441 ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg taccagtatc
     7501 aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc tctgacggat
     7561 gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt atttagccga
     7621 atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat gaaaaacgaa
     7681 aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca aaccgcagtt
     7741 tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga aacttgtggc
     7801 ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg tacacttatc
     7861 atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa agaagcttct
     7921 gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg aaaaatagat
     7981 atagagactg aactacagct cttggcccag catgatcgga tcattttcca gtttccgttt
     8041 tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt ggaacgagca
     8101 gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt ggcggaaaaa
     8161 gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag gccgtatcag
     8221 cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc acaatttatg
     8281 tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta tctctcattg
     8341 gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt acaggaaaat
     8401 cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc actgactgag
     8461 gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg aacttcatta
     8521 tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca gaatatcaag
     8581 accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa cgaacaatgc
     8641 agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga aattttctta
     8701 taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa gaatatgaag
     8761 cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg atgaaaatga
     8821 gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag ctcgtaagcg
     8881 gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag gagttgcggg
     8941 tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa cggaaacaac
     9001 gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa aagagacaca
     9061 aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa tcgtaaatga
     9121 ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc agacagaaat
     9181 tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa aagcacgtga
     9241 gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc aggcagcaga
     9301 aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta attatacgaa
     9361 tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc aagcagagtc
     9421 aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg tagaagcttc
     9481 tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta ataccaacag
     9541 cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa atcaaatagc
     9601 cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga gcatggagaa
     9661 ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat aaaaaagaac
     9721 gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt ttttcgcgta
     9781 caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat gagattctcg
     9841 tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt gtcacataat
     9901 tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct gcgtgcttct
     9961 ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca ggcaaaatac
    10021 ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt ggtagaagat
    10081 aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga taaaaaggcg
    10141 caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg gcaagtgtat
    10201 agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc gctgtaatca
    10261 cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa gtttgtacaa
    10321 aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg ataaaaatag
    10381 caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca atgatcgtct
    10441 ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct tgataggaag
    10501 caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag aatccaccat
    10561 ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca aataagatta
    10621 ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc gaaaaccaaa
    10681 attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga aacaagacga
    10741 caatgattcc gggaataatt gccggcaaat caggccacca ataattcata taagatccta
    10801 cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc caagcggtca
    10861 agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa ccagtcgaag
    10921 gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag aatcctgcaa
    10981 tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag gcacccataa
    11041 acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg gcactaagac
    11101 ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt caacaagaaa
    11161 aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat tgaactcttg
    11221 atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa actttttcat
    11281 cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa aagttaggta
    11341 aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag acctttcagc
    11401 cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc cacaaaaaat
    11461 taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat ctttgctaga
    11521 gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa actcagtccg
    11581 taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc caattgatgt
    11641 attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa gacctgggaa
    11701 aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag aatatgccaa
    11761 aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg caagagctct
    11821 agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg atgcgttaag
    11881 tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa cgattttgat
    11941 cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc ttttaaataa
    12001 acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc ttcaattggc
    12061 ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat ccaatccttt
    12121 attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc catgcttgta
    12181 ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc tttgatggga
    12241 gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt tggttttagt
    12301 tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg tttcgtgact
    12361 cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc cttttttgca
    12421 ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca tattttattt
    12481 gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt tagtggaatc
    12541 gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt cgatccgaca
    12601 atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat gttctttttg
    12661 acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat tgccatgttg
    12721 atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat gatcattaca
    12781 gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta tagttacaat
    12841 tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct tgctttttta
    12901 ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa aacaaaaaaa
    12961 tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg aaagcaggct
    13021 tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac caattcgatc
    13081 ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag tatcgtacca
    13141 gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa ggcaagcgat
    13201 gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg gttcgataac
    13261 ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag caaaaatgta
    13321 gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca tgcttggcta
    13381 gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc tgaaaaagat
    13441 gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa actaaaagaa
    13501 ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa attattagta
    13561 acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc agcttatata
    13621 tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat cattgatcag
    13681 ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc aagaagcatg
    13741 gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac tgattccata
    13801 gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa tattgaaacg
    13861 atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct agcttgagga
    13921 gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt tcgtcgtaca
    13981 gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt gtggctatga
    14041 attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg atcagccagc
    14101 aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta gctaaattat
    14161 tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata ggcaaatggt
    14221 tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt gtgttccttt
    14281 ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata accgtcaaag
    14341 catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag atccagtcgg
    14401 cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt tataatcgtc
    14461 tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca cagcaaaaaa
    14521 atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca tgggaagagg
    14581 taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt aatgcattag
    14641 ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca agaaaaaaat
    14701 ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg aaaaaagaaa
    14761 aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg acattaaaag
    14821 aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc accacggcgg
    14881 aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc catacttctg
    14941 gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt gatcagccga
    15001 aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa gtgcctaggg
    15061 atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat gatatactca
    15121 gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa tggcaaaaag
    15181 aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat gtcgctgtag
    15241 cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact gaagagttcg
    15301 gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct gataaaccag
    15361 gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca aaagcattgc
    15421 ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag acaaaaggga
    15481 tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt gatatctgcc
    15541 agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag atggaaccaa
    15601 aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat aaagacttca
    15661 aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg cagagaaacg
    15721 cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt tcagaatgtt
    15781 taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga cgtctaggaa
    15841 caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa gaaacagaag
    15901 aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag ttaaaataaa
    15961 aaaatagtta acattattca aagttt
//
LOCUS       DARDHD010000054        15975 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000054 DARDHD010000000
VERSION     DARDHD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.054"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..111)
                     /locus_tag="SDA06_001327"
     CDS             complement(<1..111)
                     /locus_tag="SDA06_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG4228611.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIV"
     gene            complement(359..1195)
                     /locus_tag="SDA06_001328"
     CDS             complement(359..1195)
                     /locus_tag="SDA06_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG4228612.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1206..2135)
                     /locus_tag="SDA06_001329"
     CDS             complement(1206..2135)
                     /locus_tag="SDA06_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG4228613.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2327..2680
                     /locus_tag="SDA06_001330"
     CDS             2327..2680
                     /locus_tag="SDA06_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4228614.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2754..3410)
                     /locus_tag="SDA06_001331"
     CDS             complement(2754..3410)
                     /locus_tag="SDA06_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4228615.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3636..5570
                     /locus_tag="SDA06_001332"
     CDS             3636..5570
                     /locus_tag="SDA06_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4228616.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5656..6879)
                     /locus_tag="SDA06_001333"
     CDS             complement(5656..6879)
                     /locus_tag="SDA06_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4228617.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6873..8771)
                     /locus_tag="SDA06_001334"
     CDS             complement(6873..8771)
                     /locus_tag="SDA06_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG4228618.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8765..9883)
                     /locus_tag="SDA06_001335"
     CDS             complement(8765..9883)
                     /locus_tag="SDA06_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4228619.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9966..10799)
                     /locus_tag="SDA06_001336"
     CDS             complement(9966..10799)
                     /locus_tag="SDA06_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228620.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10792..11709)
                     /locus_tag="SDA06_001337"
     CDS             complement(10792..11709)
                     /locus_tag="SDA06_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228621.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11732..11911)
                     /locus_tag="SDA06_001338"
     CDS             complement(11732..11911)
                     /locus_tag="SDA06_001338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228622.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(11936..13060)
                     /locus_tag="SDA06_001339"
     CDS             complement(11936..13060)
                     /locus_tag="SDA06_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4228623.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13243..14151
                     /locus_tag="SDA06_001340"
     CDS             13243..14151
                     /locus_tag="SDA06_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4228624.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14292..15905
                     /locus_tag="SDA06_001341"
     CDS             14292..15905
                     /locus_tag="SDA06_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4228625.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5065 a         3145 c         2660 g         5105 t
ORIGIN      
        1 aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa tttctactag
       61 cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca taaaaagtac
      121 ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat acatgttagg
      181 aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact
      241 ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc
      301 agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt
      361 aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa
      421 ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta
      481 ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa
      541 agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat
      601 tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt
      661 cgctagggta attataaatt agctccataa cttctaaata gttttcatct gtgatcttga
      721 tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt
      781 tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta
      841 ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac
      901 taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc
      961 cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg
     1021 cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca
     1081 ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa
     1141 cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt
     1201 ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat
     1261 aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt
     1321 gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat
     1381 cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg
     1441 ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc
     1501 tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg
     1561 tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc
     1621 ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg
     1681 attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg
     1741 gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg
     1801 ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac
     1861 gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat
     1921 tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt
     1981 tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata
     2041 agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc
     2101 atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt
     2161 tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt
     2221 acacaactct tattttttac caaagcactt caattgactt tttctgttat tcccattaaa
     2281 ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac
     2341 taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc
     2401 gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca
     2461 ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt
     2521 tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca
     2581 agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga
     2641 tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa
     2701 agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct
     2761 gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat
     2821 tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg
     2881 gtaaagttca agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct
     2941 ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac
     3001 atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc
     3061 acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa
     3121 gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat
     3181 gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat
     3241 tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt
     3301 aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa
     3361 tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct
     3421 ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa
     3481 tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct
     3541 gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat
     3601 ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga
     3661 ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag
     3721 gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca
     3781 ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg
     3841 gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga
     3901 aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta
     3961 gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc
     4021 aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc
     4081 atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac
     4141 agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag
     4201 atgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa
     4261 gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca
     4321 atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat
     4381 atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa
     4441 cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa
     4501 aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag
     4561 gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct
     4621 tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc
     4681 tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga
     4741 cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga
     4801 caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga
     4861 tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt
     4921 taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg
     4981 gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt
     5041 tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga
     5101 tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg
     5161 cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt
     5221 attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc
     5281 cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa
     5341 agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg
     5401 atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg
     5461 taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat
     5521 tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc
     5581 aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt
     5641 ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc
     5701 taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt
     5761 ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa
     5821 ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag
     5881 acctgattct attaattcta ataatccaca aaccatgatc accatactag atgtatcttt
     5941 ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa
     6001 ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa
     6061 ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga
     6121 attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat
     6181 ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat
     6241 atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa
     6301 taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg
     6361 atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc
     6421 agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt
     6481 tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct
     6541 gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt
     6601 ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct
     6661 taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat
     6721 acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt
     6781 ttccattaat tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa
     6841 catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg
     6901 tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc
     6961 catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg
     7021 gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag
     7081 tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa
     7141 cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg
     7201 attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat
     7261 tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa
     7321 gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat
     7381 acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc
     7441 ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc
     7501 atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca
     7561 aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc
     7621 aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta
     7681 gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt
     7741 agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt
     7801 tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat
     7861 atttcttttt gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat
     7921 ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga
     7981 taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag
     8041 tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct
     8101 agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga
     8161 cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat
     8221 gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca
     8281 attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac
     8341 caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga
     8401 tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt
     8461 tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat
     8521 ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag
     8581 aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt
     8641 tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca
     8701 aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt
     8761 tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat
     8821 aatcagaata gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc
     8881 ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga
     8941 gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta
     9001 attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta
     9061 cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg
     9121 ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga
     9181 atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc
     9241 cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg
     9301 atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag
     9361 agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac
     9421 tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa
     9481 tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt
     9541 tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat
     9601 gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct
     9661 ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac
     9721 cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag
     9781 aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta
     9841 cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct
     9901 tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat
     9961 tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca
    10021 taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta
    10081 attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta
    10141 tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata
    10201 agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc
    10261 acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg
    10321 ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct
    10381 gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta
    10441 aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa
    10501 cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa aactgtactg
    10561 attacagcaa taacaatcga attccaaata atcaagggat aattatatct cgccatgatg
    10621 gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt
    10681 tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag
    10741 gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt
    10801 tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt
    10861 ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt
    10921 gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg
    10981 gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg
    11041 ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc
    11101 agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt
    11161 atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt
    11221 tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt
    11281 cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat
    11341 ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc
    11401 aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt
    11461 cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac
    11521 aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat
    11581 caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt
    11641 atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga
    11701 ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact
    11761 tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat
    11821 agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag
    11881 tatttgatgt attttcattg attttattca atagttcata gtatttttgt cctccttagt
    11941 agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc
    12001 ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt
    12061 gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg
    12121 caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag
    12181 atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg
    12241 cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt
    12301 cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat
    12361 ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt
    12421 ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg
    12481 ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa
    12541 atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg
    12601 tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat
    12661 atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca
    12721 cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca
    12781 attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt
    12841 caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca
    12901 gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga
    12961 tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa
    13021 tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt
    13081 caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat
    13141 ttatataaaa aaatatttta cttgtcgttt tggcttttca tttttgttat aatagtcatt
    13201 aattataaat agaaagcttt ttatataaaa agagaaagga aagtggttct tttgttaaca
    13261 aacaaccaat cctccaatct tgtcaaaaaa acactaacct attccataga tacccatctt
    13321 catttatctg ctgctcccta ttttcgacta ctaataaatg aaagcgctaa tttatatgct
    13381 gaatttgcac ataaatctat agaactagat aatccttccc ttttattcat tgataaacag
    13441 caagaagtcc aaatcttttc tactcaaaag gaaacacaaa cttttatcat tatccagttt
    13501 tattttgatg agcaattatt agcacatatt acgggacaat ctttttctat agatcaagaa
    13561 ataacatcta tcattacttt tctgaaaaat gaacagttca aaattgatta tctaaaacat
    13621 agtaagccag ctatttccta tgatcaggaa atcaattctt ttttcatact atgccaacag
    13681 atgagcattc atttgctttc cttattagtt acttattgtc tgcaaaaaat tcaattgtct
    13741 tccttaccaa ggcattatca acaggaactt gctttattga caataccttc aaatgagaaa
    13801 gtcattccta aaaaaaagca tattctttat tcagatgaat tagttgatcc aatcatacga
    13861 tatttagaag aacatctttc agaaaatttt ttgcttgaac aattagccca aatttttttt
    13921 atcagtccag ccagcttaaa aaaacatttt agaaaagtga atggaaaaag tgttattagc
    13981 tattttaaag aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc
    14041 tatacagaaa ttgcagatat attaggattt cattcgatcc atcatttttc ttcagctttt
    14101 aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata gaaaaaaatt
    14161 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
    14221 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
    14281 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
    14341 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
    14401 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
    14461 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
    14521 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
    14581 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
    14641 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
    14701 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
    14761 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
    14821 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
    14881 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
    14941 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
    15001 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
    15061 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
    15121 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
    15181 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
    15241 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
    15301 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
    15361 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
    15421 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
    15481 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
    15541 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
    15601 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
    15661 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
    15721 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
    15781 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
    15841 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
    15901 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
    15961 aaaatgaaaa agtat
//
LOCUS       DARDHD010000055        15942 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000055 DARDHD010000000
VERSION     DARDHD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.055"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(378..800)
                     /locus_tag="SDA06_001342"
     CDS             complement(378..800)
                     /locus_tag="SDA06_001342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG4228626.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(814..2220)
                     /gene="atpD"
                     /locus_tag="SDA06_001343"
     CDS             complement(814..2220)
                     /gene="atpD"
                     /locus_tag="SDA06_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG4228627.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2243..3145)
                     /locus_tag="SDA06_001344"
     CDS             complement(2243..3145)
                     /locus_tag="SDA06_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG4228628.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3159..4715)
                     /locus_tag="SDA06_001345"
     CDS             complement(3159..4715)
                     /locus_tag="SDA06_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG4228629.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4738..5280)
                     /locus_tag="SDA06_001346"
     CDS             complement(4738..5280)
                     /locus_tag="SDA06_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG4228630.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5267..5791)
                     /gene="atpF"
                     /locus_tag="SDA06_001347"
     CDS             complement(5267..5791)
                     /gene="atpF"
                     /locus_tag="SDA06_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG4228631.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5882..6097)
                     /gene="atpE"
                     /locus_tag="SDA06_001348"
     CDS             complement(5882..6097)
                     /gene="atpE"
                     /locus_tag="SDA06_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG4228632.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6150..6869)
                     /gene="atpB"
                     /locus_tag="SDA06_001349"
     CDS             complement(6150..6869)
                     /gene="atpB"
                     /locus_tag="SDA06_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG4228633.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7171..10014)
                     /gene="rnr"
                     /locus_tag="SDA06_001350"
                     /pseudo
     CDS             complement(7171..10014)
                     /gene="rnr"
                     /locus_tag="SDA06_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10017..10772)
                     /locus_tag="SDA06_001351"
     CDS             complement(10017..10772)
                     /locus_tag="SDA06_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4228634.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10875..11111)
                     /gene="secG"
                     /locus_tag="SDA06_001352"
     CDS             complement(10875..11111)
                     /gene="secG"
                     /locus_tag="SDA06_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG4228635.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11229..11507)
                     /locus_tag="SDA06_001353"
     CDS             complement(11229..11507)
                     /locus_tag="SDA06_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4228636.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11620..12588)
                     /locus_tag="SDA06_001354"
     CDS             complement(11620..12588)
                     /locus_tag="SDA06_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4228637.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12803..12847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12871..13845
                     /locus_tag="SDA06_001355"
     CDS             12871..13845
                     /locus_tag="SDA06_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG4228638.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13850..14425
                     /locus_tag="SDA06_001356"
     CDS             13850..14425
                     /locus_tag="SDA06_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4228639.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14492..15631)
                     /locus_tag="SDA06_001357"
     CDS             complement(14492..15631)
                     /locus_tag="SDA06_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG4228640.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4355 a         3440 c         2853 g         5294 t
ORIGIN      
        1 aatgtggttc attctgactc agatttgatt ttgcacaact catcagtcct tattgacaaa
       61 gagcccaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa tcatctgacc
      121 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt gtttctctaa
      181 tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag aagtagcttc
      241 ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta aattccttct
      301 tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta aacacggata
      361 aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat tgctcggtgc
      421 aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc ttcttcgatt
      481 tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc acgttcacgt
      541 tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat tccgccatta
      601 acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac ttcatcaatc
      661 gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc agctgtcgta
      721 cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt taccacattt
      781 actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt tcgctttttc
      841 tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat catcgtattt
      901 gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat atgaacctgg
      961 aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt ggactctgcg
     1021 ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta agatagcaat
     1081 gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg ctactttata
     1141 atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca atggatctac
     1201 cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg catccaaatg
     1261 ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat agattgcttg
     1321 gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt gacccatttc
     1381 tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca aggctgatac
     1441 ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta catcttgacc
     1501 ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac gcatacgtgc
     1561 acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa ctcctgaatc
     1621 tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac cggtaaatac
     1681 agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca gtacggtttt
     1741 accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg gggcaagcaa
     1801 atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt cgtcaaaggc
     1861 tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg tttctaaatc
     1921 gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta caggaacaga
     1981 gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg ttgattccat
     2041 cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa caacttttga
     2101 tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta aggattggtc
     2161 taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc ctgaactcat
     2221 cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca atttcggtaa
     2281 tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc tcatcaatga
     2341 tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca tgttctgctg
     2401 ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc aataactgag
     2461 acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta gttgcttctt
     2521 ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta gttagcgaat
     2581 tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg tacatagttg
     2641 ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat agattgcgca
     2701 gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt ccgccaattg
     2761 cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac atcgtttgtt
     2821 tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg acgatataac
     2881 ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc cctctaggat
     2941 tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt tctcttactt
     3001 ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac ttagaagcag
     3061 acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg atacgtgtct
     3121 tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg aatggatttc
     3181 aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc tttatattcc
     3241 tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg gattgtttca
     3301 aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa gtctaaaata
     3361 ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat cactacttgt
     3421 ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt acggcgtcca
     3481 cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt aaatgcttct
     3541 aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt cattgctttg
     3601 atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc tggacgagta
     3661 cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga aataacgttt
     3721 gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa ggcagtcatt
     3781 gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg tgagtgtaag
     3841 tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag caaggaaagt
     3901 tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac atgtttcccg
     3961 ttgtacatga actcttcacc cattgcagta ccagcatatg gtgcgatata aagcaacggc
     4021 gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc atattttttc
     4081 aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac atagatacag
     4141 atcatatctt gacctttttg gttgatgatc gtatcgatcg caatggaagt tttccctgtt
     4201 ttacggtcac cgataactaa ttcacgttgg ccgcgtccga ttggtacaag agcgtcgatt
     4261 gcttttagac ctgtttgcat tggttcgtta acagatttac gttgcataac gcctggtgcc
     4321 attgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc aattggctga
     4381 cctagcggat taactacccg tccaatcaag gcctctccaa ctggaacttc catgatcttt
     4441 cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag gataatgata
     4501 cctacatcat ttgattctaa gttttgcgcc attccgtatg agccatttga aaattcaagc
     4561 aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc acctacataa
     4621 gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat ttgttcttta
     4681 atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct ctttgtacta
     4741 tctatttaat tgattacgca tattttctaa ttgcgtgcga atgcttccgt cgattactcg
     4801 atggttagct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt gttttaattc
     4861 gactgattga tagttcattg tttttgcaac attcttttct aattgtattc gttgttcgtc
     4921 tgaaagtgga actgctgttg tgacacttcc caacagcaaa ccttgatgtt catcatatcg
     4981 acgttcatat tcttcgatca tgaacaaaag atcatccatt cggttatacg cataaacaac
     5041 ttctaaaaag tttttaacga ttccatcata gccactaaca agcttgtcca tgatttctcg
     5101 tttttcatga ggttctagac gaacatcact taacatatta cctaatccag gtacttgttc
     5161 atagatttga cgtaagctta cgagatcttg ataaatttct tctgctttat ttgaatcgat
     5221 cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat ctagtttcat
     5281 ttgaagaacc tagaccttcg atatattgat tgattaatga ttcgtgcatt tctggagata
     5341 attctttgtt caagattttt tccgcgattt gaagagatag ttctgcaaca tcgtctttta
     5401 cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggcttta ctttttagac
     5461 gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct ccactttctt
     5521 tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt tgttcgcgtt
     5581 cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatccaaa tcattggcga
     5641 tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa tgcttcaaga
     5701 gagccagtag tatcaaaaat gagccgctga caacgatgac atttcctaac atcgcattgc
     5761 caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt tgaagtactt
     5821 ctagttttca tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa tactgattca
     5881 attaaacacc aaaaactaga atcaaggcga caactacccc taagatagga acagcttcga
     5941 ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca ggttggcgag
     6001 ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct ccgattgctg
     6061 ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc ctaaattaga
     6121 taaatagtag tttgttttta aaacgaatat tattcttcca cttcaatctt atggctcata
     6181 taaaccatag ataaagtaac gaagacaaat gcttggatgc ttccgataaa caaggagaat
     6241 gcgatccaga ccatctctaa tggaattgct aacggcaatg agaaccagcc taagcttatt
     6301 accatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc ataaagacgt
     6361 aaagcaagtg ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa cataaaacta
     6421 tatggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt caccccaaat
     6481 aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg gtctgctgtc
     6541 gggcttttcc aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa accgatgttg
     6601 tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt gctcaattct
     6661 tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca ttctatcgca
     6721 ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc taagaagaaa
     6781 acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca aattggtcca
     6841 atgtggaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc gaaattcaca
     6901 taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca cacggtatca
     6961 taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact tttttatttc
     7021 agtcaaaaac gaagcgaatt tttctttata cacggtgctc ctgatctaag aaaaaaccta
     7081 tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt aaaagcaaaa
     7141 gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta atgttcgatc
     7201 aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcataag attttttccc
     7261 tttcgccaga ccaatcaata ccttggcata gccgttacga atatagactt ttaaaggaac
     7321 aacagtgatt ccagtatttt tcaattcatt ttcaagccgg atgatttgtt ttttgtgtaa
     7381 taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt cataaggact
     7441 gatatgaaca ttatgcaaga aagcttctcc gttgcggacg cgaataaatc catcttttag
     7501 attgatccgg ctgtttcgta tcgacttgat ctcggttcct tgcaagacca tccctgcttc
     7561 catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa ttaattttcc
     7621 ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt tcttcttttt
     7681 aacacctttg taaaatggct tcttcccttt tttcttaccg aacgtttgct tactatcgct
     7741 atatcgtttg ttcttcttgc catttctttc tgggcgtttt cctgcttgtt ttccttcgcg
     7801 ttttcctgat tttccatttc gtgtttttgg ccgttccaac gctgtaacct cttctgcgga
     7861 aacaagttca aaatctactg ctcgttcatc tggatcagct ttgactactt ttactcgcac
     7921 tttctgtccg attttaaaag tcagaccagt tctttcacct acaagagcta aatgattttc
     7981 aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatccct ctactgtatt
     8041 cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat cgaattcttc
     8101 ccccactttg tccatcatga attctgcttt cttcatggaa tctacttcac gttctgtttc
     8161 tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt ccgtagccca
     8221 cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac gatggacgat
     8281 tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg ccgctaagcc
     8341 ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta gcaacatagt
     8401 attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct gtaaatcttt
     8461 tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag caaaatcaaa
     8521 gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa acggcagatt
     8581 taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag attcgatcaa
     8641 gcgttcacca attcctctgc ttcttagctg gatatcattt gggtgtcctt cagcatcaac
     8701 aaggattttt gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc gcatcgtttc
     8761 aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag catagcgttg
     8821 catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg tcatccgttc
     8881 tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat ttggatcaat
     8941 ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac agataccatt
     9001 cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac ttgtccctct
     9061 ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa catctgcaat
     9121 gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg catcatctag
     9181 atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat ctctacgtcc
     9241 agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca atacatcatc
     9301 aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca tgccagggtc
     9361 attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat accctttttc
     9421 agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa ctccctctgc
     9481 tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa cgccgtaaag
     9541 atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta actgagtagt
     9601 cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat ctgtatttgc
     9661 tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag tcgcttcttt
     9721 tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc ctcgttcatt
     9781 tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt ttcctttttt
     9841 attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta gtaatttaaa
     9901 atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa aactgcgttt
     9961 gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg tcatatttat
    10021 tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa ttcgcgtctt
    10081 gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt cactcgctga
    10141 cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc atctttccca
    10201 gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac gattgctgcc
    10261 tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac tgctttttca
    10321 ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta tttttcaaag
    10381 ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc gccgccaatc
    10441 actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa tcctaatacg
    10501 gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt gtctgcccac
    10561 cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg tcctttaaaa
    10621 agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat tcgaacatcg
    10681 tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg ttttgcataa
    10741 aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta ccatagtttc
    10801 tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg ccgttttgcg
    10861 agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa gatcatccag
    10921 atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg agctttttgt
    10981 ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt ttgcttgctt
    11041 ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat tcccaaaatt
    11101 aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact ttatcataga
    11161 taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa aactttcagc
    11221 tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc aaactctttt
    11281 tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa ttctactcca
    11341 gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac aaatgtatgg
    11401 tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat ctcttgttgt
    11461 tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg ccgcactcct
    11521 caaataactg atattttttt attcctacct tattttagca gtctcctgat ctataaacaa
    11581 ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg ttattctttg
    11641 ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg atgcttccaa
    11701 gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat tttgaatcgt
    11761 gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca ataaaggcag
    11821 atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac cggcgtattt
    11881 ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag tgattttact
    11941 taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa taaatccttt
    12001 gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc ccattgatcc
    12061 gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga tccaggcaat
    12121 taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt ctccgtgcgc
    12181 acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac ctatatgaga
    12241 catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt tattctgctt
    12301 atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt tgattgtttt
    12361 gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc gttttttttc
    12421 tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat accagtcata
    12481 tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa gaaaatggta
    12541 actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg tacacccgcc
    12601 gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag gattctttat
    12661 tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa gttctcctca
    12721 atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata ctaatttcgt
    12781 gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa taaaaaacta
    12841 agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga ttgcgatcca
    12901 gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat cgaagtagtt
    12961 gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa tgtgctgaaa
    13021 tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga aaaacctttg
    13081 aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg ggaaacaaat
    13141 ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt tttggctgat
    13201 tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac caatatcgct
    13261 tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt ctcgatgggt
    13321 ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta ctggtgtgat
    13381 cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat ggttgggtta
    13441 gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg tcaaatgaat
    13501 cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt ccactggaaa
    13561 caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta ttttatcgag
    13621 ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg aataagtcgt
    13681 ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag taaaattatg
    13741 acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt aaatgcacaa
    13801 gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa tgaataaaaa
    13861 caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca attatattgg
    13921 aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg gaacgatttt
    13981 tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca gcggtatttt
    14041 aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa ttctcacagg
    14101 cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca gcttgaaact
    14161 gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga tcattaccgt
    14221 tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc tattacatgg
    14281 gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag actaccttga
    14341 tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac aacgcttgat
    14401 atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat aatgctaatc
    14461 tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa ataattcctt
    14521 gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct acttgatgag
    14581 gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta gctttcgtaa
    14641 attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg ctgaattgtt
    14701 gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt ttgttgtaat
    14761 cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg attgattgca
    14821 gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc ccttggagct
    14881 gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta acattatgga
    14941 ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt tcagcatttg
    15001 ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct ggcatagagc
    15061 tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg tgcgtataca
    15121 tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct acatagcctg
    15181 ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg gctaataatt
    15241 tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca atcaccattg
    15301 ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc ccatggattt
    15361 ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg acatccatat
    15421 gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca atcaaattat
    15481 ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc aaaagcttat
    15541 tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg atgatctctt
    15601 gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca tttcttttta
    15661 ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa tgaagaaaga
    15721 gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata cgagaggact
    15781 tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga tgctagttat
    15841 tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta aacatcatat
    15901 acagaagcta gttgcaaaaa aaactctcta cctgctgata tt
//
LOCUS       DARDHD010000056        15942 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000056 DARDHD010000000
VERSION     DARDHD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.056"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..1480
                     /locus_tag="SDA06_001358"
     CDS             56..1480
                     /locus_tag="SDA06_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4228641.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1779..2486
                     /locus_tag="SDA06_001359"
     CDS             1779..2486
                     /locus_tag="SDA06_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4228642.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2473..2796
                     /locus_tag="SDA06_001360"
     CDS             2473..2796
                     /locus_tag="SDA06_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4228643.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2885..3922)
                     /locus_tag="SDA06_001361"
     CDS             complement(2885..3922)
                     /locus_tag="SDA06_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4228644.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4243..5226)
                     /locus_tag="SDA06_001362"
     CDS             complement(4243..5226)
                     /locus_tag="SDA06_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG4228645.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5641..6411)
                     /locus_tag="SDA06_001363"
     CDS             complement(5641..6411)
                     /locus_tag="SDA06_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228646.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6404..7339)
                     /locus_tag="SDA06_001364"
     CDS             complement(6404..7339)
                     /locus_tag="SDA06_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228647.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7655..9304)
                     /locus_tag="SDA06_001365"
     CDS             complement(7655..9304)
                     /locus_tag="SDA06_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4228648.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9421..12024)
                     /locus_tag="SDA06_001366"
     CDS             complement(9421..12024)
                     /locus_tag="SDA06_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4228649.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12036..13085)
                     /locus_tag="SDA06_001367"
     CDS             complement(12036..13085)
                     /locus_tag="SDA06_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG4228650.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13111..13425)
                     /locus_tag="SDA06_001368"
     CDS             complement(13111..13425)
                     /locus_tag="SDA06_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG4228651.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     YK"
     gene            complement(13441..13887)
                     /locus_tag="SDA06_001369"
     CDS             complement(13441..13887)
                     /locus_tag="SDA06_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4228652.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13888..15810)
                     /locus_tag="SDA06_001370"
     CDS             complement(13888..15810)
                     /locus_tag="SDA06_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG4228653.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4708 a         3241 c         2656 g         5337 t
ORIGIN      
        1 tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa aaattatgac
       61 aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt ggtcagctga
      121 gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc gctggtttaa
      181 agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg atcacatcca
      241 tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct ttcgcgtttc
      301 tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat
      361 ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag tatttgccaa
      421 cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg aatcacgtga
      481 agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg gtgacttagt
      541 ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt atcttgaaga
      601 ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat tcttcactat
      661 acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta gtaaaatagg
      721 cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc
      781 agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg ttaaagggaa
      841 tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg aagattatac
      901 gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac taaactatta
      961 cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc catttttacc
     1021 agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat accggggatg
     1081 ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc attatggaaa
     1141 catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac gtttcatgga
     1201 tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc atcttgctta
     1261 cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt ggacatttat
     1321 tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt cactcgacct
     1381 tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat taagtgatac
     1441 aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa ttccacttga
     1501 agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac tttttcttca
     1561 tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt
     1621 aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt ttttatttgt
     1681 ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag tttgacgaca
     1741 attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag ttagatataa
     1801 aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt ggacttgcat
     1861 ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg atgtccctgc
     1921 tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc catagtccac
     1981 tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg atgagtatga
     2041 cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc
     2101 tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga caaaagttaa
     2161 actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg ggttcttctc
     2221 ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt ttggatttcg
     2281 ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat agaaacacct
     2341 ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat gtcggactga
     2401 tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc ggattggggg
     2461 tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg ttgtacttta
     2521 gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga tttgccgcaa
     2581 ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt atggttcagc
     2641 agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa tgctttggct
     2701 tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat cgtcttggta
     2761 ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga gcttatggca
     2821 aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat cggcgtattc
     2881 ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt cctgtttcta
     2941 cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag accttgctca
     3001 atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt
     3061 gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag tcgaagctga
     3121 tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg atcgttccca
     3181 catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta atatcgaata
     3241 atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta cttaagtcgt
     3301 gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa ttcttcggac
     3361 ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa atcgaaccga
     3421 cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt tcttctggag
     3481 tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg
     3541 cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg tcaaccagtt
     3601 ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga
     3661 cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca tcaaacccta
     3721 agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg acaggattaa
     3781 cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt atctgttctt
     3841 tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa ccgactacct
     3901 tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt ggagcatcca
     3961 tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt catcataatt
     4021 attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag
     4081 acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt attcgctcaa
     4141 aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac
     4201 agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag
     4261 cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag tagatttcgt
     4321 attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa
     4381 gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc
     4441 tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg gttctttttc
     4501 aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta cgccatattt
     4561 ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg tgatcagtgt
     4621 ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc aatgtgcatc
     4681 tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg
     4741 aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt
     4801 gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg ctacttctgg
     4861 gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat
     4921 gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa
     4981 ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg taaaggtcaa
     5041 tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc
     5101 agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt
     5161 tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt
     5221 agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa
     5281 cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc
     5341 tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc cggaataaat
     5401 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat aggaggagct
     5461 atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact
     5521 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa atatgaggaa
     5581 ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     5641 ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac
     5701 gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg ttccaaggaa
     5761 tccgtaacgg aaaccggaga tcatataaac gatcggattg actttcgaaa cagcttgcca
     5821 aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa
     5881 gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc cgttgagcag
     5941 tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga
     6001 atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat
     6061 actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag gaacaggcgc
     6121 taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg aagaaacgtt
     6181 ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga attccatata
     6241 tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa taacaaagta
     6301 caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca aatagcggtt
     6361 cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct
     6421 ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga ttcgacttat
     6481 tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt ccgttcaccc
     6541 cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta caagcgtatc
     6601 cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca
     6661 gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat tggatgatcc
     6721 cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa atgatcgttg
     6781 taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat tcaatatcga
     6841 ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa
     6901 tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc
     6961 aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac
     7021 cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga ttgaaattga
     7081 actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta tccagatcat
     7141 aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg attccgatcg
     7201 ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct tctttcacgg
     7261 aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc aaattattta
     7321 tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct ttattgtatc
     7381 atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt tgctgggctc
     7441 aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt ttcagaaaaa
     7501 acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt gagacaaaag
     7561 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca aaagccaaga
     7621 tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc cactgccgct
     7681 tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct gggtaatcca
     7741 aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat
     7801 caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat
     7861 tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact
     7921 ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgtccctt
     7981 ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg tccaacaaac
     8041 gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga
     8101 ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt
     8161 ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg
     8221 atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc actaccgctt
     8281 cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc
     8341 ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact tctaattctc
     8401 ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga
     8461 aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaactctg
     8521 cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct cttaatggtt
     8581 tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc tgattggcgg
     8641 ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct
     8701 tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag attcctagta
     8761 cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga aaaataaata
     8821 aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat
     8881 gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt tgaggatttt
     8941 cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca aagctgcgta
     9001 aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt ccttcgaata
     9061 cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc catgaacgga
     9121 acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat ctcggttgat
     9181 aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt gaaaaaatgt
     9241 ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat tctctttgac
     9301 ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg cgctatttga
     9361 aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta tattacatat
     9421 ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg ttaattcttg
     9481 tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag gtactatttt
     9541 ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa aataagaagg
     9601 ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt tacgaggaac
     9661 atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa atacattcag
     9721 tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt ttgccacttt
     9781 gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat actcacctat
     9841 ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat ctcctgaagc
     9901 tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg aaaataaggt
     9961 tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat aaaacgtaaa
    10021 ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat taacaggttt
    10081 ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt caataaatcc
    10141 gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg ctaagcgcat
    10201 acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt gactttctcc
    10261 cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag ataaacgatc
    10321 ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt gtaaagaatc
    10381 tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt ctaatggtga
    10441 ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat caagatattg
    10501 tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat atttattgtt
    10561 acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat ttttctcaaa
    10621 ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt caagttcata
    10681 ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag catttttccg
    10741 ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga agtgtatttt
    10801 ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag tcgttgtgtt
    10861 gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt ttacaataag
    10921 gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa tatcttccgc
    10981 aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca aaatcttttt
    11041 ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg caatcagcat
    11101 gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta ttttcatttt
    11161 tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac gaagttcacc
    11221 tttatattca ggtaattctt ttttgtttct gataacttct acataagaag gataatctga
    11281 aataatattg tggaatttac tgtatttatt caacttctct acgacttgat caaaatttaa
    11341 aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt ttccttgcat
    11401 atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa tatctttaat
    11461 tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg cataaacaaa
    11521 tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt caaagtttaa
    11581 tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag gcaaattcgc
    11641 attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat atttaaggat
    11701 cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca ctaataaggc
    11761 atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca gtcgttggat
    11821 aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag tttgtccatc
    11881 taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag taaagacctg
    11941 atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc agtgcgtgtg
    12001 ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc tactgaattt
    12061 acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa cgctgtaacc
    12121 actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa aggaattgcc
    12181 ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag accacatgct
    12241 actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac ggcaaacggg
    12301 atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc attatctcgt
    12361 tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc aaatggcgga
    12421 atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga aaaagaagca
    12481 accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc catcattcct
    12541 cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag ccaattagtc
    12601 agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat gattccaaca
    12661 actaatgtag taacaaaagg aatcagcata attgatttta gactaataac ttgcatcggt
    12721 acttttactt ttttcaatgc attagctaaa tagccacaag ctaatccagc aaccattcct
    12781 ccaataaatc ccataccaag attttgacac atcatcccag tgataaaagc tggtgctaga
    12841 ccgggacggt cagcaattga atatgcgata taggctgaaa tcatcggtac gataaactgt
    12901 aatcccgtct gaccaatcat tcgcaggact ccccagaatg tatccgtctg gtcccaaaca
    12961 cctgtgccac caaaaatgac tgcaatagaa gttagtaacc ctgctgctac gacaagcggg
    13021 atcatatagg aaaccccact ttggaaatga tttagaattt ctcttccttg tttcttgaat
    13081 tccattagta actcccccac gatatatttt ttatttgtaa attaatgcaa ttgcttcatc
    13141 aatcgtctct tcaccatctt ttactacctc tgaggtccct actgttaaaa tcggtttacc
    13201 gtcaaagcga cctcgttctg aaacatttgt atctactgca aatatgacaa catctgcctc
    13261 ggaaacatcc gaaattgaaa gtttgttttc tatccccata gccccctgtg tttctacttt
    13321 aatatctaac cctttcttct tcgcaaactt ttctaaatta gcttttgcca tataagtatg
    13381 ggcaatccct gcgatacatg ctgttactgc tactattttt gtcatatgaa attcctcctt
    13441 ttaaaatgat agagctttta ccaattcttt tttatttctc gaatctttta ttcttttaac
    13501 aaactcgtca tccatcaatc ttccagataa ttctgctaaa atacgtaaat gattagtacc
    13561 tttatcagtg tcttttattg ctaacaaaaa gacgagatca actggttgat catctaatgc
    13621 gccccattca acagaccgct ttaacttggc tacagccact gttgattcaa ttactgctag
    13681 gcttttgcca tgaggaatgg cgatattttt cccaattcct gtggtcatat ggttttctct
    13741 gtcaaatacg tctttttgaa attgtttgtg gtcagacaca aacccattag tctctaacaa
    13801 tttcgataat ttttcgacta cacttttttt atcagatgca tttaaatcta tcactatatt
    13861 atttatattg attttttcac tgatcattta taagccttct ttcaatatat aaactaattt
    13921 ttcaaacgac gaactcgttt ttaaatttgc taataactca tgattcgaag cgatttcatt
    13981 gaatgtttca taaattttat tcaattcagg ttttactttt tcattaagtg caaaaaagaa
    14041 aacagaatga acaagtgtcc catcccaatc aattccttct ggattaacgt agacacagat
    14101 tactggcttt tttataaaat ctacttcgat atgcggcata gcaaaatcat tcatcgctgt
    14161 tgaagatagt ttttctcttt tgattgctcc ctctagtatc ccttctttcg cgtaattttc
    14221 ttttaccaat tttttggtaa ttatatttaa aacgttcact tgattatcct ttatttggtg
    14281 ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa gcatcttctg tttgttcatt
    14341 taactgtaga tactgtttta cttttctgac atctgcccct aacatcaaag gactaacaac
    14401 aataacagga aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt catcagtgac
    14461 ttttctatct tgtagttcat tgatgcttag tacatcttct atctcaacca tatctgcaaa
    14521 accgttattt attctttgct ctaataattt tgatgttcca tatccgctac tacaaactaa
    14581 tatcactttc gttttgtcag gtttgtaacg atccaaaagt gattgaatat ggagtgttac
    14641 gtaagcgatt tcatcttcat taaaatgtat acagaatctc tcttcgattt cctctaataa
    14701 atctacactt atcataaatg accttttaaa agaatatttg attttgtccg tataagggtt
    14761 atagatattt actcccaatt tgagtctttt aactgctgca ttgagatgta atactagatt
    14821 attgatcaat tcttcatcag gataaaattc atttaattta attcttacta actcttgtag
    14881 ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat tcatagcatt
    14941 tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg gcacaataat
    15001 tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga tttcttcttc
    15061 agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg caatagcaag
    15121 gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct tattcatgta
    15181 tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat tattcgcttc
    15241 accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg ctgtaaagcc
    15301 accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt catcagcttt
    15361 gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta gtctttttct
    15421 aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt cagcaagatt
    15481 ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag ttagacggtc
    15541 ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa gtgtttcttt
    15601 ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat ctggcatttt
    15661 ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag atacatataa
    15721 ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt atagtatttt
    15781 catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac agcttaagtg
    15841 tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta gaagtttcta
    15901 atattcttga ttatttcaaa tcaataaaaa agcctgcaat at
//
LOCUS       DARDHD010000057        15534 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000057 DARDHD010000000
VERSION     DARDHD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.057"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            48..3215
                     /locus_tag="SDA06_001371"
     CDS             48..3215
                     /locus_tag="SDA06_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228654.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3235..3780
                     /locus_tag="SDA06_001372"
     CDS             3235..3780
                     /locus_tag="SDA06_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEG4228655.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            3808..4263
                     /locus_tag="SDA06_001373"
     CDS             3808..4263
                     /locus_tag="SDA06_001373"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEG4228656.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            4260..5018
                     /locus_tag="SDA06_001374"
     CDS             4260..5018
                     /locus_tag="SDA06_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4228657.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(5104..5844)
                     /locus_tag="SDA06_001375"
     CDS             complement(5104..5844)
                     /locus_tag="SDA06_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228658.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5905..7158
                     /locus_tag="SDA06_001376"
     CDS             5905..7158
                     /locus_tag="SDA06_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4228659.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7480..7707
                     /locus_tag="SDA06_001377"
     CDS             7480..7707
                     /locus_tag="SDA06_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4228660.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7830..8156
                     /locus_tag="SDA06_001378"
     CDS             7830..8156
                     /locus_tag="SDA06_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4228661.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            8224..8937
                     /locus_tag="SDA06_001379"
     CDS             8224..8937
                     /locus_tag="SDA06_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG4228662.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            9247..10098
                     /locus_tag="SDA06_001380"
     CDS             9247..10098
                     /locus_tag="SDA06_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4228663.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            10219..12084
                     /locus_tag="SDA06_001381"
     CDS             10219..12084
                     /locus_tag="SDA06_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4228664.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            12132..13541
                     /locus_tag="SDA06_001382"
     CDS             12132..13541
                     /locus_tag="SDA06_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG4228665.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            13649..14350
                     /locus_tag="SDA06_001383"
     CDS             13649..14350
                     /locus_tag="SDA06_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4228666.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14509..14988
                     /locus_tag="SDA06_001384"
                     /pseudo
     CDS             14509..14988
                     /locus_tag="SDA06_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5188 a         2643 c         3365 g         4338 t
ORIGIN      
        1 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
       61 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
      121 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
      181 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
      241 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
      301 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
      361 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
      421 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
      481 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
      541 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
      601 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
      661 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
      721 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
      781 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
      841 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
      901 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
      961 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     1021 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     1081 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
     1141 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
     1201 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     1261 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
     1321 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
     1381 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
     1441 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
     1501 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
     1561 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
     1621 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
     1681 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
     1741 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
     1801 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
     1861 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
     1921 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
     1981 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
     2041 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
     2101 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
     2161 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
     2221 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
     2281 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
     2341 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
     2401 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
     2461 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
     2521 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
     2581 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
     2641 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
     2701 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
     2761 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
     2821 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
     2881 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
     2941 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
     3001 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
     3061 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
     3121 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
     3181 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
     3241 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
     3301 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
     3361 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
     3421 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
     3481 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
     3541 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
     3601 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
     3661 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
     3721 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
     3781 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
     3841 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
     3901 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
     3961 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
     4021 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
     4081 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
     4141 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
     4201 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
     4261 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
     4321 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
     4381 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
     4441 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
     4501 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
     4561 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
     4621 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
     4681 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
     4741 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
     4801 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
     4861 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
     4921 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
     4981 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
     5041 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
     5101 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
     5161 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
     5221 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
     5281 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
     5341 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
     5401 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
     5461 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
     5521 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
     5581 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
     5641 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
     5701 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
     5761 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
     5821 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
     5881 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
     5941 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
     6001 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
     6061 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
     6121 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
     6181 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
     6241 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
     6301 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
     6361 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
     6421 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
     6481 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
     6541 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
     6601 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
     6661 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
     6721 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
     6781 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
     6841 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
     6901 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
     6961 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
     7021 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
     7081 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
     7141 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
     7201 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
     7261 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
     7321 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
     7381 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
     7441 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
     7501 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
     7561 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
     7621 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
     7681 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
     7741 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
     7801 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
     7861 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
     7921 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
     7981 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
     8041 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
     8101 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
     8161 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
     8221 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
     8281 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
     8341 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
     8401 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
     8461 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
     8521 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
     8581 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
     8641 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
     8701 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
     8761 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
     8821 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
     8881 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
     8941 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
     9001 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
     9061 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
     9121 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
     9181 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
     9241 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
     9301 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
     9361 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
     9421 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
     9481 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
     9541 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
     9601 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
     9661 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
     9721 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
     9781 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
     9841 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
     9901 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
     9961 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    10021 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    10081 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    10141 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    10201 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    10261 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    10321 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    10381 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    10441 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    10501 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    10561 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    10621 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    10681 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    10741 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    10801 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    10861 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    10921 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    10981 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    11041 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    11101 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    11161 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    11221 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    11281 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    11341 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    11401 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    11461 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    11521 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    11581 aatcaattag aaaacaaaca tgcgaatgca ggaaaaccga ttacttcagc acggcatacc
    11641 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    11701 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    11761 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    11821 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    11881 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    11941 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    12001 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    12061 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    12121 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    12181 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    12241 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    12301 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    12361 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    12421 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    12481 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    12541 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    12601 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    12661 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    12721 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    12781 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    12841 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    12901 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    12961 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    13021 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    13081 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    13141 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    13201 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    13261 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    13321 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    13381 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    13441 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    13501 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    13561 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    13621 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    13681 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    13741 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    13801 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    13861 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    13921 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    13981 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    14041 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    14101 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    14161 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    14221 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    14281 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    14341 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    14401 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    14461 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    14521 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    14581 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    14641 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    14701 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    14761 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    14821 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    14881 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    14941 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    15001 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    15061 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    15121 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    15181 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    15241 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    15301 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    15361 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    15421 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    15481 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacg
//
LOCUS       DARDHD010000058        15517 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000058 DARDHD010000000
VERSION     DARDHD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.058"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SDA06_001385"
     CDS             138..1475
                     /locus_tag="SDA06_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG4228667.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SDA06_001386"
     CDS             1523..3652
                     /locus_tag="SDA06_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG4228668.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SDA06_001387"
     CDS             3627..4316
                     /locus_tag="SDA06_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228669.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SDA06_001388"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SDA06_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SDA06_001389"
     CDS             complement(5546..5740)
                     /locus_tag="SDA06_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228670.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SDA06_001390"
     CDS             complement(5790..6230)
                     /locus_tag="SDA06_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG4228671.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SDA06_001391"
     CDS             complement(6234..7079)
                     /locus_tag="SDA06_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG4228672.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SDA06_001392"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SDA06_001392"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG4228673.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SDA06_001393"
     CDS             complement(8703..9650)
                     /locus_tag="SDA06_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4228674.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SDA06_001394"
     CDS             complement(9873..10223)
                     /locus_tag="SDA06_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4228675.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SDA06_001395"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SDA06_001395"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG4228676.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SDA06_001396"
     CDS             11646..11966
                     /locus_tag="SDA06_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4228677.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SDA06_001397"
     CDS             11988..12452
                     /locus_tag="SDA06_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4228678.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SDA06_001398"
     CDS             12657..13262
                     /locus_tag="SDA06_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG4228679.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SDA06_001399"
     CDS             complement(13301..14590)
                     /locus_tag="SDA06_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG4228680.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SDA06_001400"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SDA06_001400"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG4228681.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4845 a         2899 c         3182 g         4591 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaa
//
LOCUS       DARDHD010000059        15128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000059 DARDHD010000000
VERSION     DARDHD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.059"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..489)
                     /gene="yajC"
                     /locus_tag="SDA06_001401"
     CDS             complement(109..489)
                     /gene="yajC"
                     /locus_tag="SDA06_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG4228682.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(586..1731)
                     /gene="tgt"
                     /locus_tag="SDA06_001402"
     CDS             complement(586..1731)
                     /gene="tgt"
                     /locus_tag="SDA06_001402"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG4228683.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1949..2647
                     /locus_tag="SDA06_001403"
     CDS             1949..2647
                     /locus_tag="SDA06_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4228684.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2815..5454)
                     /locus_tag="SDA06_001404"
     CDS             complement(2815..5454)
                     /locus_tag="SDA06_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4228685.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6073..7104)
                     /gene="queA"
                     /locus_tag="SDA06_001405"
     CDS             complement(6073..7104)
                     /gene="queA"
                     /locus_tag="SDA06_001405"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG4228686.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7297..8472
                     /locus_tag="SDA06_001406"
     CDS             7297..8472
                     /locus_tag="SDA06_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4228687.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8476..9117
                     /locus_tag="SDA06_001407"
     CDS             8476..9117
                     /locus_tag="SDA06_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228688.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9114..10031
                     /locus_tag="SDA06_001408"
     CDS             9114..10031
                     /locus_tag="SDA06_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228689.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10028..10699
                     /locus_tag="SDA06_001409"
     CDS             10028..10699
                     /locus_tag="SDA06_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228690.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11240..11728)
                     /locus_tag="SDA06_001410"
     CDS             complement(11240..11728)
                     /locus_tag="SDA06_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG4228691.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11929..12195)
                     /locus_tag="SDA06_001411"
     CDS             complement(11929..12195)
                     /locus_tag="SDA06_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4228692.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12195..12458)
                     /locus_tag="SDA06_001412"
     CDS             complement(12195..12458)
                     /locus_tag="SDA06_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4228693.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12934..13119
                     /locus_tag="SDA06_001413"
     CDS             12934..13119
                     /locus_tag="SDA06_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4228694.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13261..15084)
                     /locus_tag="SDA06_001414"
     CDS             complement(13261..15084)
                     /locus_tag="SDA06_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG4228695.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4382 a         3243 c         2852 g         4651 t
ORIGIN      
        1 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
       61 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
      121 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
      181 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
      241 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
      301 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
      361 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
      421 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
      481 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
      541 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
      601 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
      661 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
      721 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
      781 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
      841 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
      901 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
      961 tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
     1021 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
     1081 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
     1141 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
     1201 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
     1261 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
     1321 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
     1381 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
     1441 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
     1501 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
     1561 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
     1621 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
     1681 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
     1741 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
     1801 gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat
     1861 gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg
     1921 tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc
     1981 aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt
     2041 gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat
     2101 atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg
     2161 cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac
     2221 ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg
     2281 tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt
     2341 tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc
     2401 tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact
     2461 ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt
     2521 agatctttca tttatcggct ggcatatctt agcattagca actgctggta ttggttatct
     2581 atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga
     2641 tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag
     2701 aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca
     2761 cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc
     2821 ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac
     2881 gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag
     2941 ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat
     3001 ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac
     3061 attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac
     3121 atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc
     3181 aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca ataaacctcg
     3241 agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg
     3301 ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg
     3361 cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc
     3421 cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg
     3481 tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc
     3541 ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt
     3601 caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg
     3661 ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc
     3721 tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc
     3781 ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg
     3841 atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt
     3901 actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc
     3961 cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat
     4021 cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc
     4081 tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc
     4141 gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac
     4201 tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac
     4261 gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt
     4321 cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc
     4381 ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg
     4441 tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc
     4501 tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt
     4561 caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt
     4621 catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc
     4681 agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg
     4741 tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat
     4801 gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac
     4861 catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc
     4921 aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat
     4981 caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac
     5041 cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt
     5101 attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa
     5161 taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc
     5221 tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa
     5281 atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt
     5341 attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg
     5401 atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt
     5461 cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta
     5521 tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct
     5581 tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat
     5641 caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac
     5701 aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg
     5761 tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta
     5821 aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac
     5881 aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt
     5941 acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag
     6001 ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg
     6061 cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc
     6121 ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa
     6181 catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa
     6241 ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt
     6301 aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt
     6361 cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa
     6421 ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa
     6481 ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa
     6541 ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata
     6601 gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat
     6661 ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg
     6721 tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat
     6781 tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg
     6841 tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa
     6901 acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc
     6961 aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa
     7021 acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa
     7081 atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc
     7141 tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc
     7201 ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga
     7261 tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca
     7321 aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt
     7381 gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc
     7441 aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa
     7501 attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg
     7561 cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa
     7621 gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg
     7681 ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt
     7741 gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca
     7801 atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg
     7861 atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc
     7921 atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg tcatcctgca
     7981 aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa gccaaatgcg
     8041 acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga aaaatcatta
     8101 caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt taccgataat
     8161 tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg aggaaaagta
     8221 tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc cgcctatctt
     8281 cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt cgttgatgaa
     8341 cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt ctacgatgtg
     8401 atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc tgaagaatct
     8461 aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat tctctcaaaa
     8521 ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat cattgctgta
     8581 ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat tggcttaacc
     8641 agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc gctatttggc
     8701 gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc gattttacgt
     8761 aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc aaaaggcatg
     8821 gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat gccaacgatc
     8881 atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact tgcctcttat
     8941 atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta ccaaccagat
     9001 ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga ttggcttcta
     9061 ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga agaatgaaac
     9121 gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt tgttccttcc
     9181 ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga atcacttcag
     9241 aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca gataaaaata
     9301 ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg atgaacggag
     9361 atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca ttgaaccttc
     9421 ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt gagaaaagat
     9481 acggtcaaac gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt cttgttcgaa
     9541 aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat gtagcggatg
     9601 aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt tatgacggct
     9661 tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt ggacttgttt
     9721 atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg gatggacgga
     9781 tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca ccatatgatg
     9841 ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa gaagccttaa
     9901 ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac gaagcagata
     9961 ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac cactattttg
    10021 aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct attatttcca
    10081 ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt ctatctatgg
    10141 tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta ggagacgtaa
    10201 attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt ctctagccat
    10261 gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg ttactgtatt
    10321 tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc aagtagacaa
    10381 aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac tattcatggt
    10441 agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt tagttgttgc
    10501 catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag atatcattat
    10561 ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat tgccaactgc
    10621 attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc tagatccagc
    10681 cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac catttgttgg
    10741 tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg attcttccct
    10801 ccttatgggt aaagagttgc ttttcttagc tatttatcca ttccgtatac tccaacttca
    10861 tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat ccgctaaggc
    10921 attttgccaa tccattttcc catcactcgt catggaattc ataccacatg atctctaaat
    10981 actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc gtggctttca
    11041 tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc atcaaaactg
    11101 gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt gccatcctca
    11161 tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt ttgcggttcc
    11221 taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg gcgatacgat
    11281 gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc caataagaaa
    11341 agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata ctgctattca
    11401 tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg ttcggcaatg
    11461 tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc gtatctgtat
    11521 cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac tccggttgat
    11581 tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca tttccaattg
    11641 tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact aacaacgatg
    11701 ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca taaacccgtc
    11761 aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata ggaacaaatt
    11821 aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag agtctccttt
    11881 ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc actcataatg
    11941 tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt aaaccactcg
    12001 atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca gcttttcaaa
    12061 agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt caaattgttt
    12121 ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt tgattgcgac
    12181 tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt aaagctactg
    12241 tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag aaaaagggtt
    12301 tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc actacgatca
    12361 ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg ataaaaattt
    12421 ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc tttttctgat
    12481 ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac ttaaattcag
    12541 caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg atctttgtcc
    12601 acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa ttggtaacaa
    12661 cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac aacaccaata
    12721 cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct gaggatacgc
    12781 acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt ctactgcctc
    12841 aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt gatacttgct
    12901 tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg taacaaaaaa
    12961 tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt aagagtcgag
    13021 gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg attcggaaat
    13081 tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat aataacattt
    13141 catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag aataagacaa
    13201 aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt aaaacttggt
    13261 ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc aagacaatcg
    13321 caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc tgtttggtac
    13381 aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt tacttacgat
    13441 atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga cgattgaaaa
    13501 acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt tttcgacatc
    13561 ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg cgtaattcaa
    13621 cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat tcccgtcata
    13681 atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt tataatgatc
    13741 atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag gccaaatgtc
    13801 ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg cacccgtaat
    13861 attggcaatc gatttgctta accatctttt tccttctttt ttccatttga cgaccatccc
    13921 tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa tcaagcgttc
    13981 tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg tgataatccc
    14041 attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga atttatcttt
    14101 tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag caactgctaa
    14161 aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa agacagcttt
    14221 accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc aatagttgat
    14281 aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca atgtagcagc
    14341 ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg cctccacccc
    14401 agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag aaacacccgg
    14461 cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca cgatattata
    14521 taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg gaaataatag
    14581 gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca aaacgattgt
    14641 cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga tcgcttcagc
    14701 acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag agccaccgga
    14761 aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc ccccgccatg
    14821 aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata aaataataac
    14881 aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga ctaaaacgac
    14941 aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca ataatgccaa
    15001 agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag gcttacctac
    15061 taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt tacttcaagt
    15121 tttatagt
//
LOCUS       DARDHD010000060        14875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000060 DARDHD010000000
VERSION     DARDHD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.060"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..1173
                     /locus_tag="SDA06_001415"
     CDS             7..1173
                     /locus_tag="SDA06_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228696.1"
                     /translation="MINFIVYLIYYLLMVIMAVIAQDTLHASLSQAGFASGIYIIGTL
                     LARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVST
                     ATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVF
                     FVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFL
                     SSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLL
                     SVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVG
                     PYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1321..1686)
                     /locus_tag="SDA06_001416"
     CDS             complement(1321..1686)
                     /locus_tag="SDA06_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG4228697.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1833..2606)
                     /locus_tag="SDA06_001417"
     CDS             complement(1833..2606)
                     /locus_tag="SDA06_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4228698.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2780..3640)
                     /locus_tag="SDA06_001418"
     CDS             complement(2780..3640)
                     /locus_tag="SDA06_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG4228699.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3640..5058)
                     /locus_tag="SDA06_001419"
     CDS             complement(3640..5058)
                     /locus_tag="SDA06_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4228700.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5115..5564)
                     /locus_tag="SDA06_001420"
     CDS             complement(5115..5564)
                     /locus_tag="SDA06_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4228701.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6009..6272)
                     /locus_tag="SDA06_001421"
     CDS             complement(6009..6272)
                     /locus_tag="SDA06_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG4228702.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6371..7666)
                     /gene="rho"
                     /locus_tag="SDA06_001422"
     CDS             complement(6371..7666)
                     /gene="rho"
                     /locus_tag="SDA06_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG4228703.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7663..8934)
                     /locus_tag="SDA06_001423"
     CDS             complement(7663..8934)
                     /locus_tag="SDA06_001423"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4228704.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9099..9968)
                     /locus_tag="SDA06_001424"
     CDS             complement(9099..9968)
                     /locus_tag="SDA06_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG4228705.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10260..11870)
                     /locus_tag="SDA06_001425"
     CDS             complement(10260..11870)
                     /locus_tag="SDA06_001425"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG4228706.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13063..14403)
                     /gene="glnA"
                     /locus_tag="SDA06_001426"
     CDS             complement(13063..14403)
                     /gene="glnA"
                     /locus_tag="SDA06_001426"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG4228707.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14431..14823)
                     /locus_tag="SDA06_001427"
     CDS             complement(14431..14823)
                     /locus_tag="SDA06_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4228708.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4179 a         3209 c         2569 g         4918 t
ORIGIN      
        1 atcacattga tcaattttat tgtctacctg atttattatc tgttgatggt catcatggca
       61 gtcattgcgc aagacacctt acacgccagc ttgagccaag caggttttgc ttctgggatc
      121 tatatcatcg gaacattgct ggcacgctta ttcatgggga aacagttaga actatttggt
      181 cggaagttta ccttaagagg cggcgcgatc ttctatctcc tttcaacatt agcatatctt
      241 tatacgccaa ctatcggggc attgtattta gttcgattct tgaatggatt cggctacggt
      301 acagtttcta cagctacaaa tgctatcgtt actgcatata tccctgcatc taaaaaaggg
      361 gaaggcatca attattatgg attaagtact agtttagctg ccgctgttgg gccattttta
      421 ggaatgattt tgctgaatat gactgatttt cgtttcatta tctggttttc aattgtactg
      481 gtcttctttg ttacgattgc ctgccttgtc tttcctgtaa aaaatattcc attatctgaa
      541 gaacaaaaaa tatctcttcg ctcatggaat atcaatagtt ttattgaaaa gaaagcactt
      601 ttcattacag gaattgcctt tttgatgggt ctttcttatt caagtgtttt atcattttta
      661 tcttcttata caaaagtaat ccacttagtt gctgctgggt catttttctt cgtcgtatat
      721 gctttggtca tcacatttac acgtccattg actggaagga ttttcgatgt caaaggagaa
      781 caatacgtga tgtatcctag ctatttattc ttaacagcag gattattttt actgagtgta
      841 acgacgaata gtgtgacatt gcttatctct ggagcattag tcggcctagg ttatggtacg
      901 tttatgtcaa atggacaggc tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt
      961 gctttatcta cctactttat tggtttagac ctaggcttag gcgttggtcc ttatttgatg
     1021 gggatgctaa aatctcttac ttcatttaga ggattgtatg tgattgccgg agtcattcca
     1081 ttgatatgta cagcactcta tcttgttaat ggccgaaaac atagtcatga agcagagggc
     1141 gaattgattg aatcattgaa aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt
     1201 tcagatctga aaataatctg agcggttttt tgagtaaaaa tagtttatga ttattcttaa
     1261 ttaaacatct atttattttt aaagctttca tttatcgata agaatagttt aactgttgat
     1321 ttattccatt ttccgttcat ctggtacgat gccttgactt agcaaccact tgtggaagaa
     1381 ccattctgcc acaccgatca atagagcagg aatcaatact ttcatccaaa tatcgactgt
     1441 atcattcata aaatatagat agagccacaa gataactgca gctgaaccag catctgcgat
     1501 cgtcgctgcg atgttcccta ctttacgtaa gatcaacaga tcccctagca catagatcat
     1561 gactacaata attaccccaa caatcgttga atccataaag ctaacaccgt ataaacctgt
     1621 cagcacgacc cacagaatcg cccagatcat gattgccttc actaccaacg ccttcacatg
     1681 tttcattttc tgcactcctt tctcctggat aaaaaagtaa gattatctaa caagataacc
     1741 ttttcattat tataccatcg gctgagagaa gcgagcaaag ataaggtcct atctaaaaat
     1801 gaggaagcca cttagaacat tcacgtaata atttatccgt acagacattc gatatggtgt
     1861 tcctttactt tcgcatactg ttcttttgtc agttctttac ttgtcaccag ataatcgatt
     1921 tcatgaaaag caatggcttg atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa
     1981 acggcaagcc ggtctgattg ttttctaaaa gcttttttga ccattgcctc tccttcatga
     2041 gtttctgtta ctccttggct gaaatctagc ccgcgacaac tcattatcgc taaatccgca
     2101 taatatgaca atgcatcgtt ataggctttt gaaccgttga ttgttgctcg tttcggaaca
     2161 attgcccctc ccaatacaga tacttttgcc gttgtatatt cactcaacat agcagctgtg
     2221 acgatgccat tcgtaatgat ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt
     2281 aaagaggttg tactggaatc caagaataag accatatcat ctcgaatcaa agaagaagct
     2341 gtatctgcaa tcttacgttt ttctgtttct tcttctaatt tggtgatctt catcaggtaa
     2401 ggcacgtcaa tttttcgatt gtcaataagc atggcgccac cgcgaactct acggatcaat
     2461 ccactttttt ccagaaaaac aaggtcacgt ttaatcgtgg cttcgctata atttactaac
     2521 tcgatcaaat cggtgactct tgcaaattgg ttgtttttta gataattcac aatctcctgc
     2581 tgtctattct ccacttcatt caacataaaa tcaccccatt tactttatat agtaacagga
     2641 aaaaagaggt tgtgacagaa gtgattggct tcgagaaata agaagaaatt tttcgaaaaa
     2701 actacttctg aacatcgtgt gtggttaaga gggtatgaca agactattgt cataccctct
     2761 tgaccacaca tttttatttt tagtataaac tagccttgcc tactgtgttc aaaatttcca
     2821 ttttatgttt gaccacttct tgtacctttt tattagctgc tggataaatt gcattcggct
     2881 cgtacatcga tggattttca ctcaatattt ctctcaaagc attgaaaaag acgcttttaa
     2941 gatctgagct caaattcact ttggcaactc cgtattttac tgcttcgctg acttctgcgt
     3001 ccggattacc tgaaccgcca tgtaaaacaa atggaagatc gatttgttgg ttcaattctt
     3061 tcagtaagtc gatatttaat tttggagttc tgtttttcgg atacaagcca tgcgctgttc
     3121 caattgctac agcaagtgta tcgattcctg tttctttcac aaaaatcttc gcttgatcag
     3181 gatctgtata aatgattgtt tcagcacctc cttcagcaga accattattc ccaatcgttc
     3241 caagctcagc ctccactgag acatttaccg catgagctaa cgcaaccact tttttcgtaa
     3301 tctgtacatt gtcgtcgaat gatttcaaag acgcatcgat catcacggaa gaatagccgt
     3361 ttcgaatcgc ccgtaagaca tcttctatcg taccgccatg atccaagtgg ataacgatcg
     3421 gcagtttact tgtataagca taggctctta ctgccgcaac aaactcatca cttaaatagg
     3481 cgatttcatc aggatgaatt tctaagatta ctggtgcttc taatctctct gcttcttcga
     3541 tgattgcttt catcatatca tagctgcaaa tattgaaggc tggtactgca aattgattct
     3601 ctttagccac tttcaataac tctttcattg tatataacat tacgcttcca cctcatttgt
     3661 tagattttca tttttcgtcc acttgtcttg ttttttctct ttcactgttt ttttcaatag
     3721 tccaagcata aaggctgtag tgactgtacc tgatgcaatt gccaagacat acataggtaa
     3781 attgttcata acatttggaa ttagcatcac ccaaatccct ccatgaggtg ccattgaagt
     3841 aactcctact gccatggaaa tccctgcagc gactgctgaa cctgtcatca aactaggtat
     3901 cacgcggatc gggtcagata ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc
     3961 taaaatccag catgattgac ctgaagtccg ttcttctttg gtaaatttat gtttgaataa
     4021 taaagtggct aaagcaatac ctaggggagg tgtcattcct gcagccatac aagcagcaat
     4081 cggtgcattt actccagctg acatcaaacc gatcgagaaa gtcgaagtcg atttattgat
     4141 cggcccaccc atatcagctg ccatcatgat cccgataagt aatccgaaca atagcccgga
     4201 tgtatttccc aagctattca gccaatttgt caaagcatcc aacacccatg tgatcggttg
     4261 ttcaatcaca aataccatca aaagcccaat aattaagatc gataaaaagg ggacgacaat
     4321 cagctgataa atagcagaaa attctttttt tacgctaatt ttatttgcca gaaaatcaca
     4381 gatataccct gctagcacgc caccaatcat tccgccaaga aaacctgaac cacctgtaga
     4441 ggccagtaat ccggcaacaa ttcctggggc aaaacctgct tttccagcaa tcgatgtagc
     4501 aatccccgcg cctaacgcag gaaccatcat ggcaaacgct gtatctcctc cgatccttga
     4561 aaatgcagct gctaacacat tatagtctgc tgagttggga tcagccgctg taatcccaaa
     4621 ggcaaaactc agtgcgatga tgattccgcc tgcgatcaca aatggaagca tgtaattcac
     4681 accattcatg aaatgattat agattgtcgg ctttttctca tttgcagctg gttctttttc
     4741 attagacaca acgaatacgc caatatctct caatgcttcg tctattacta cttgcggttc
     4801 tttgattgct cggcttgtag agacacgctt tacttttttc ccggcaaatc ggtccaattc
     4861 gatttcttta tctactgcaa gaatgattgc atctgcctct ttgatttctt gttcagataa
     4921 ctgattttct gtttttacac cttgtgtttc gaattttgct tgatggttta gatttcttgc
     4981 tgttacttct aatttttctg ccgacatata agtatgagca attccagtcg ggcaagcagt
     5041 tacaccaacg attttcatga ttattttcct cctttttctt cactatcgat actcggacaa
     5101 agactctact attttcattc ttgactaatc tgtctgatga tatctgctgg cgtagtggct
     5161 tcttttattc cctttctaaa atctgcattc atcaatcgtc tggagagtgt gctgagaatc
     5221 tctagatgca tatctgctga tttttcagga actacgatgg caaaaatata ttcaattggc
     5281 atttcatcta tcccgctata gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt
     5341 tttgtcacat atttgctttt tgcatgggga atcccaaatc catcttctat tcctgtggag
     5401 atctcctcct ctcgtttgaa tagtgcctta acgacttttt gaacatttct taccttattt
     5461 gtttttttca acacatcccc tattcgctca aataatgatc cttttgaatc tatcgatgga
     5521 tcgaaaacaa tcagttcttc tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc
     5581 acaaaaaatg ttaacgtttt cttggaactg aggtcagtat agcatcgcta gctgtaccgt
     5641 tcaatattat gaatgaacag aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa
     5701 tgaacgatct agctcacaaa caatcgcaag tctataacaa aacaaaaagc cttccttgaa
     5761 aggaaagctt agcttctaat tttaggtaaa tggtttttta cttcgaaata acccctcttc
     5821 atttcgttaa tataaggtgt ttttatcaag tggataatta aaactatata ctagtttttc
     5881 attgttttgt gcattatgat cctacgactt tttcagcatc aaacgaagtt ttaaattggc
     5941 aaaaaacata aaaaaacaga ctatccctat cagatagtct gctaaacgtt gagttatcag
     6001 cgtttttatt attccgcagc gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc
     6061 acacgtccgt ctgcttgtgt gaatttttga cgtccagtgt agaatggatg tgagtcagaa
     6121 gttacttcga cacggatgac tgggtatgta ttaccgtctt cccattcaac tgtttcttgt
     6181 gaagtttttg ttgaacctga caagaatttg aaaccagttg ttgaatccat gaatactact
     6241 gggtggtaat ctggatggat gttttctttc atgatttttt cgctcctttg ccctgctcca
     6301 tttgctgaac cagagttgat ttgtcgattt acaacattaa catcttaaca tgtttcagtc
     6361 aaggttgcaa ttatcttttc agatttctgg tcggattttt tttaccaaag gagacatctt
     6421 gaaattcttt gaaaaattgc tgattgtttt ttgttttacg caaaaagcgg atcagttgtt
     6481 ccgtatattc taatgaatct ccagtcatat tttttcgaag tttccaaatt tcttctaatt
     6541 cttcacttgg catcaataat tcttcttttc gggtacctga ttttttgata tcgatggctg
     6601 ggaagatccg acgttcagat aattcacgtg aaagttgtaa ttctagattt ccagttcctt
     6661 tgaattcttc atagatcacg tcatccatac ggcttcctgt atcgactaaa gcggtcgcta
     6721 aaatcgtcaa actgccacct tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt
     6781 tatacaatgc ggctgggtca atcccgccac tcaatgtacg tccgcttggt ggaacgacta
     6841 agttataggc ccgagcaagt ctagtaatac tatccatcaa gataacgaca tctcgtttat
     6901 cttcgactaa acgcattgcg cgttccaata ctaattcaga aactcgagta tggttttgcg
     6961 gctgcagatc aaatgtagaa gataccacgt cgcctttcac gcttcgttct agatccgtta
     7021 cttcttccgg tcgttcatca atcagtagaa cgattaattc tacttccgga tagttttcgg
     7081 tgataccatt tgcgatttct tttaatacag aagttttccc tgcttttggc ggtgcgacga
     7141 tcaatccacg ctgaccaaaa ccgattggag aaaagatatc gatcattcgg gtagatagtc
     7201 tttgcggtgt tgtttccagt tgaagctgtc tttgcggata aagaggtgtt aaggctggaa
     7261 aatgcggacg ttcttttgct tcttccggat cttttccatt aacgctttcc acgtgcatca
     7321 aaccataata acgttccgat tctttaggtg gacgggcttt tcctgccact ttgtccccat
     7381 ttctcaaact gaatcgtctg atttgtgagg cagaaatata gatatcttct gcacttggtc
     7441 cataattgat tggccgtaaa aaaccgtaac catcttgacc aacgatatcc aatattcctt
     7501 ccatataata aaatccttgt ttctctgctt gtgcacgaat cacagctaga gataattcct
     7561 ttttattcat cttactgtaa taaggaattt taaattcttt ggcataacta taaacttctt
     7621 ttaacgtttt gttctctagt tctgccattg tcaaataatc gctcattcag ctcccacttc
     7681 ctctatcatt tcaattgatg cccctaaggc tgttagtttc tcgataatat gatcataacc
     7741 gcgtaagata taatctacat tgtaaatcgt cgttgtccct tctgccaata gtccagcaat
     7801 cactaaacaa gcacctgccc gaagatcaga agccacgact tctgtgccgt gaagtttatt
     7861 tggaccattc acgatgatca tatttccttc cacggtaata tcagcgccca ttcgtgctag
     7921 ttccggcaca tggttcacac gtttcgcata gatcgtatcg atgatttctg aagtgcctgt
     7981 agtcatcaat agtaacgcag taagtggctg ctgcagatct gtggcaaatc ctggataagg
     8041 ataggtcatg atgtttgcgg gttttaattc tttcgagggg taaacttcga tttcatcttc
     8101 gctgattttc atatggacac caatttcctg caattttgct ataaagcttt ccaaatgttc
     8161 aaagatcaca tttttcactt tgataccatt tcctactgca gctgctaatg ctagataggt
     8221 tcctgcttcg atgcggtcag gaatcatgaa atgccgacag ccatgtaatg tttcgactcc
     8281 ttcgatacgg atcacatctg tacccgctcc tcgaactttt gcccccatat tattcagtaa
     8341 agttgccaca tctatgattt ccggttctcg tgcagcgttt tcaattactg tctgtccttt
     8401 tgcttttaca gcagctaaca tcacattgat cgttgctccg actgaaacca cgtccatgaa
     8461 aatccgattg ccacgcaatc ctttattttc tgtccgtaaa tacatggcac catgttcatt
     8521 tgtcacttca gcacctagag cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc
     8581 taaataacag ccacctggca ggcctactac cgcttcgcca aatttaccaa gcagtgttcc
     8641 cataaaataa taagaagcac gcaaactatt gatttttccg tgcggcatcg gaatcgatac
     8701 gacttctcga ggatcgattt ctagtatgtt gttggaaaaa tggacagttg cgcccatgat
     8761 ctctaaaatt tcaattaatg aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac
     8821 tggggaatca gctaagattg cagctggaat caatgcgact gcactatttt ttgcaccgct
     8881 gatcgttact tcaccagcta aaggtcggtt tcctttgatt acgatttttt tcatctgttt
     8941 ccaccttgtt tattaatctt ttttcatgga tatgcttgta ttttatcata gataaaaaaa
     9001 agaaacgagc atggtctgcc gttttaagta aaaaagaatg gagagcgact tcaaaagtcg
     9061 ttctccattc ctatatatcg tatctgttat gtggatgctt acgctttgtt agctgaaccg
     9121 aaccagtcga tatgagattt agcatcttca acgattgctg ctttacctgg agctaataat
     9181 ttacgagggt cgaatccttt accttcttta tctttaccag cttcgatata tttacgagta
     9241 gctgcagcaa atgataattg gaattcagtg ttaacattta cttttgagat acccattgag
     9301 atagcttttt cgatttgatc ttgaggaata cctgatccgc catgtaatac taaaggcatg
     9361 tctggaccta cagcatcagc aattgcttgt aagtggtcaa aagctaatcc tttccagttt
     9421 tctggatatt gaccatggat gttaccgata ccgcaagcta agtagtcgat acctgtagca
     9481 accatttgtt tgcattcttc aatatcagct aattcgccgt taccgatgat tccgtcttct
     9541 tctccgccga ttgaaccaac ttcacattca actgagatac cttttgcatg tgctttttca
     9601 acaacgtctt tagctaattt caagttttct tcaaatggta gatgtgaacc atcgaacatt
     9661 actgaagtat agcctaattc gatacattcc aaagcagctt cgtaatcacc atggtctaag
     9721 tggatagcaa ctggaacagt gatgttcatt gcttcaacta aatcagtgat taaatcttta
     9781 cagattttgt aaccacccat gtatttagct gcacccattg atgtttggat aagtactggt
     9841 gcttgttttg cttcagccgc ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg
     9901 aatccgccga cagcgtaacc gcctttacga gcagctttta aaaattcagc tcctgatact
     9961 actggcataa taaaattcct cctaagtttt gtaaaactaa atttgttaag acaattctta
    10021 accaacgacc ctattttacc aaacatttcg gtagttttct actaaaatca atgaaaatga
    10081 aaattttcaa gaagtttttt gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa
    10141 gtcttttgct ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg
    10201 acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat
    10261 tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg
    10321 cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct
    10381 ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct
    10441 tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct
    10501 tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat
    10561 gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt
    10621 aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat
    10681 ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa
    10741 aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga
    10801 tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt
    10861 tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa
    10921 ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta
    10981 ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc
    11041 cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc
    11101 attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg
    11161 ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa
    11221 acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct
    11281 tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc
    11341 aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt
    11401 gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc
    11461 acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata
    11521 tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact
    11581 tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa
    11641 cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct
    11701 ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg
    11761 atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct
    11821 tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa
    11881 actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt
    11941 caataagaat agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag
    12001 gtgtataata caagctgact aagggaaagt caagtactca aatgaatact atccgcttac
    12061 aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa
    12121 aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa
    12181 tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca
    12241 ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt
    12301 tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa
    12361 aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa
    12421 acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta
    12481 gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg
    12541 tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    12601 tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg
    12661 taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta ttaagccaaa
    12721 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc gaaactaaac
    12781 gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt
    12841 cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta cttgttgcag
    12901 ggcagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
    12961 ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa tactcaggtc
    13021 aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa gttctagata
    13081 ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga gacgttttgc
    13141 ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat tatccttacg
    13201 caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg cagcttctct
    13261 ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg tgatctgatt
    13321 acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg ggtttgcaga
    13381 tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg gtacacgtac
    13441 taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat aacctggaac
    13501 gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac gagcatgttt
    13561 cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac cgtctttatc
    13621 atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac cagaaccatt
    13681 gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt
    13741 tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat ctgcatattt
    13801 aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc
    13861 tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc
    13921 aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa
    13981 tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg
    14041 catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc
    14101 cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt cccacgggaa
    14161 gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa
    14221 tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt cgatctggct
    14281 cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa
    14341 tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct gtttctttac
    14401 catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt gcaatccttg
    14461 cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat
    14521 ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg
    14581 tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa gatagtcttt
    14641 aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc cttcgcttcg
    14701 ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag acaaatcagt
    14761 cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt ccttttctcc
    14821 catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa aaaaa
//
LOCUS       DARDHD010000061        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000061 DARDHD010000000
VERSION     DARDHD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.061"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SDA06_001428"
     CDS             complement(296..1024)
                     /locus_tag="SDA06_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG4228709.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SDA06_001429"
     CDS             complement(1002..2576)
                     /locus_tag="SDA06_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4228710.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SDA06_001430"
     CDS             2805..3743
                     /locus_tag="SDA06_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228711.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SDA06_001431"
     CDS             3755..4666
                     /locus_tag="SDA06_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4228712.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SDA06_001432"
     CDS             4690..6162
                     /locus_tag="SDA06_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4228713.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SDA06_001433"
     CDS             6213..6824
                     /locus_tag="SDA06_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4228714.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SDA06_001434"
     CDS             6854..8767
                     /locus_tag="SDA06_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG4228715.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SDA06_001435"
     CDS             8895..11876
                     /locus_tag="SDA06_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG4228716.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SDA06_001436"
     CDS             11873..13534
                     /locus_tag="SDA06_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG4228717.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SDA06_001437"
     CDS             13574..14506
                     /locus_tag="SDA06_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG4228718.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARDHD010000062        14456 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000062 DARDHD010000000
VERSION     DARDHD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.062"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..543)
                     /locus_tag="SDA06_001438"
     CDS             complement(109..543)
                     /locus_tag="SDA06_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228719.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(643..1491)
                     /locus_tag="SDA06_001439"
     CDS             complement(643..1491)
                     /locus_tag="SDA06_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4228720.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1880..3646
                     /locus_tag="SDA06_001440"
     CDS             1880..3646
                     /locus_tag="SDA06_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG4228721.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            3748..5007
                     /locus_tag="SDA06_001441"
     CDS             3748..5007
                     /locus_tag="SDA06_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4228722.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            5154..6455
                     /locus_tag="SDA06_001442"
     CDS             5154..6455
                     /locus_tag="SDA06_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228723.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            6458..7309
                     /locus_tag="SDA06_001443"
     CDS             6458..7309
                     /locus_tag="SDA06_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4228724.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            7322..8137
                     /locus_tag="SDA06_001444"
     CDS             7322..8137
                     /locus_tag="SDA06_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG4228725.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            8288..9454
                     /locus_tag="SDA06_001445"
     CDS             8288..9454
                     /locus_tag="SDA06_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4228726.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            9684..10331
                     /locus_tag="SDA06_001446"
     CDS             9684..10331
                     /locus_tag="SDA06_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4228727.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            10415..11359
                     /locus_tag="SDA06_001447"
     CDS             10415..11359
                     /locus_tag="SDA06_001447"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG4228728.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            11494..12945
                     /locus_tag="SDA06_001448"
     CDS             11494..12945
                     /locus_tag="SDA06_001448"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG4228729.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            12966..13802
                     /locus_tag="SDA06_001449"
     CDS             12966..13802
                     /locus_tag="SDA06_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4228730.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            13802..>14456
                     /locus_tag="SDA06_001450"
     CDS             13802..>14456
                     /locus_tag="SDA06_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4228731.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     "
BASE COUNT         4745 a         2554 c         2871 g         4286 t
ORIGIN      
        1 ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca
       61 aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt
      121 ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca
      181 taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt
      241 catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc
      301 agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc
      361 aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc
      421 attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg
      481 atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg
      541 cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct
      601 aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata
      661 agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc
      721 gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa
      781 aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc
      841 aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac
      901 agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat
      961 agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac
     1021 gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat
     1081 gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc
     1141 taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt
     1201 ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa
     1261 gggtttttta cctaaaaata gaagagctag taccaacata acgatattca acactaaaat
     1321 aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc
     1381 tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag
     1441 ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact
     1501 tccttaattc attaataata ctcttatctt attataactt acttttcacc ttaagttaac
     1561 tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata
     1621 cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct
     1681 taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta
     1741 agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat
     1801 aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta
     1861 aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata
     1921 tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat
     1981 caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca
     2041 aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga
     2101 agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac
     2161 ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc
     2221 aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg
     2281 ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc
     2341 aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt
     2401 cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa
     2461 tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga
     2521 ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga
     2581 agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca
     2641 ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt
     2701 ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag aaactgctga
     2761 aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa
     2821 tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat
     2881 tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag
     2941 agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc
     3001 tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc
     3061 gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa
     3121 tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc
     3181 tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt
     3241 gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg
     3301 gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca
     3361 agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa
     3421 gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta
     3481 tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc
     3541 ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga
     3601 cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt
     3661 aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc
     3721 acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta
     3781 gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca
     3841 gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa
     3901 gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta
     3961 gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag
     4021 ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat
     4081 ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg
     4141 acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat
     4201 aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa
     4261 gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg
     4321 actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat
     4381 gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct
     4441 tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca
     4501 ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca
     4561 tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact
     4621 ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat
     4681 tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat
     4741 caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca
     4801 gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa
     4861 gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc
     4921 gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc
     4981 agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat
     5041 ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt
     5101 gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc
     5161 aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga
     5221 tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc
     5281 tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg
     5341 gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca
     5401 tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag
     5461 cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata
     5521 taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta
     5581 cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc
     5641 atattccacc agccagttta attgattggg taggggctaa aaactttttt agtatcttct
     5701 ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt
     5761 tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga
     5821 aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag
     5881 cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata
     5941 ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga
     6001 aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc
     6061 cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag
     6121 cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg
     6181 tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct
     6241 ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta
     6301 ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta
     6361 tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat
     6421 ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc
     6481 tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt
     6541 cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc
     6601 agcttttagt ttagatcttt caagtgactg gactttagga aactttactc ggctattcag
     6661 tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat
     6721 cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg
     6781 ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt
     6841 gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac
     6901 aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga
     6961 cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat
     7021 ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc
     7081 ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa
     7141 tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc
     7201 tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt
     7261 acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat
     7321 catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc
     7381 gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt
     7441 gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca
     7501 atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga
     7561 attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt
     7621 ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga
     7681 gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa
     7741 ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt
     7801 agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct
     7861 tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg
     7921 gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat
     7981 gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga
     8041 ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt
     8101 tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac
     8161 aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa
     8221 cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag
     8281 gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc
     8341 tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc
     8401 ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcatc
     8461 tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc
     8521 attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa
     8581 atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca
     8641 ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg
     8701 tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca
     8761 tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg
     8821 gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact
     8881 aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa
     8941 atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac
     9001 aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt
     9061 aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg
     9121 cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt
     9181 tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt
     9241 acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga
     9301 tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc
     9361 ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc
     9421 tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac
     9481 tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct
     9541 aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc
     9601 tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt
     9661 ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag
     9721 ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt
     9781 ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt
     9841 cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg
     9901 cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg
     9961 aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag
    10021 aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc
    10081 tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat
    10141 tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa
    10201 cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg
    10261 gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac
    10321 aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac
    10381 tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa
    10441 tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga
    10501 attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa
    10561 tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc
    10621 tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa
    10681 agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga
    10741 aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa
    10801 tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta
    10861 tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc
    10921 ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac
    10981 ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc
    11041 agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac
    11101 tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg
    11161 tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat
    11221 gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta gaatcataaa
    11281 agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa
    11341 attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat
    11401 aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg
    11461 tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa
    11521 gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata
    11581 aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc
    11641 cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta
    11701 aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa
    11761 acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt
    11821 ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc
    11881 tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga
    11941 gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt
    12001 tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg
    12061 atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg
    12121 acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt
    12181 gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag
    12241 atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct
    12301 gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac
    12361 ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca
    12421 gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa
    12481 cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat
    12541 tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat
    12601 ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt
    12661 gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac
    12721 gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat
    12781 ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta
    12841 ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta
    12901 tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa
    12961 gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg
    13021 cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga
    13081 aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa
    13141 atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca
    13201 aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat
    13261 cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt
    13321 cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa
    13381 tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata
    13441 tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc
    13501 agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc
    13561 agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt
    13621 tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa
    13681 tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat
    13741 attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt
    13801 gatgtacttt cccctaattg atttttccct catgtggact tcacttcctt tcgtactgca
    13861 aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa atttggtagg
    13921 gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt
    13981 ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt tatatttcgg
    14041 gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag
    14101 tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga
    14161 cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca
    14221 agcttttcga atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac
    14281 ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg
    14341 tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg
    14401 tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc agtcaa
//
LOCUS       DARDHD010000063        14381 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000063 DARDHD010000000
VERSION     DARDHD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.063"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(27..923)
                     /locus_tag="SDA06_001451"
     CDS             complement(27..923)
                     /locus_tag="SDA06_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4228732.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="SDA06_001452"
     CDS             complement(916..1596)
                     /locus_tag="SDA06_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228733.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="SDA06_001453"
     CDS             complement(1597..2379)
                     /locus_tag="SDA06_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228734.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="SDA06_001454"
     CDS             complement(2372..3289)
                     /locus_tag="SDA06_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4228735.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3776)
                     /locus_tag="SDA06_001455"
     CDS             complement(3453..3776)
                     /locus_tag="SDA06_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228736.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="SDA06_001456"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="SDA06_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG4228737.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="SDA06_001457"
     CDS             7904..8218
                     /locus_tag="SDA06_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4228738.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="SDA06_001458"
     CDS             8231..8605
                     /locus_tag="SDA06_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4228739.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="SDA06_001459"
     CDS             8615..8950
                     /locus_tag="SDA06_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG4228740.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="SDA06_001460"
     CDS             9033..10373
                     /locus_tag="SDA06_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4228741.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="SDA06_001461"
     CDS             10451..11125
                     /locus_tag="SDA06_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG4228742.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11118..11282
                     /locus_tag="SDA06_001462"
     CDS             11118..11282
                     /locus_tag="SDA06_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228743.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11358..12500
                     /gene="dcm"
                     /locus_tag="SDA06_001463"
                     /pseudo
     CDS             11358..12500
                     /gene="dcm"
                     /locus_tag="SDA06_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12490..12780
                     /locus_tag="SDA06_001464"
     CDS             12490..12780
                     /locus_tag="SDA06_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228744.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /locus_tag="SDA06_001465"
     CDS             13039..14223
                     /locus_tag="SDA06_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4228745.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14220..14357
                     /locus_tag="SDA06_001466"
     CDS             14220..14357
                     /locus_tag="SDA06_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4228746.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4596 a         2632 c         3019 g         4134 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt t
//
LOCUS       DARDHD010000064        14280 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000064 DARDHD010000000
VERSION     DARDHD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.064"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            50..325
                     /locus_tag="SDA06_001467"
     CDS             50..325
                     /locus_tag="SDA06_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG4228747.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            331..1098
                     /locus_tag="SDA06_001468"
     CDS             331..1098
                     /locus_tag="SDA06_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG4228748.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1266..3095
                     /gene="typA"
                     /locus_tag="SDA06_001469"
     CDS             1266..3095
                     /gene="typA"
                     /locus_tag="SDA06_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG4228749.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3356..5128
                     /gene="recQ"
                     /locus_tag="SDA06_001470"
     CDS             3356..5128
                     /gene="recQ"
                     /locus_tag="SDA06_001470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG4228750.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5242..5544
                     /locus_tag="SDA06_001471"
     CDS             5242..5544
                     /locus_tag="SDA06_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG4228751.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5547..6710
                     /locus_tag="SDA06_001472"
     CDS             5547..6710
                     /locus_tag="SDA06_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4228752.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6736..10164
                     /locus_tag="SDA06_001473"
     CDS             6736..10164
                     /locus_tag="SDA06_001473"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG4228753.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10185..11309
                     /locus_tag="SDA06_001474"
     CDS             10185..11309
                     /locus_tag="SDA06_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228754.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11299..11736
                     /locus_tag="SDA06_001475"
     CDS             11299..11736
                     /locus_tag="SDA06_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4228755.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11720..12058
                     /locus_tag="SDA06_001476"
     CDS             11720..12058
                     /locus_tag="SDA06_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG4228756.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12177..12737
                     /gene="rsmD"
                     /locus_tag="SDA06_001477"
     CDS             12177..12737
                     /gene="rsmD"
                     /locus_tag="SDA06_001477"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG4228757.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12734..13225
                     /gene="coaD"
                     /locus_tag="SDA06_001478"
     CDS             12734..13225
                     /gene="coaD"
                     /locus_tag="SDA06_001478"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG4228758.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13215..14258
                     /locus_tag="SDA06_001479"
     CDS             13215..14258
                     /locus_tag="SDA06_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG4228759.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         4856 a         2434 c         3114 g         3876 t
ORIGIN      
        1 ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata
       61 tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg
      121 ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta
      181 tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt
      241 tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa
      301 caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc
      361 tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa
      421 aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc
      481 gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat
      541 caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat
      601 tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga
      661 aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata
      721 ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc
      781 ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg
      841 gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg
      901 aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat
      961 gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt
     1021 gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt
     1081 ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca
     1141 aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc
     1201 tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag
     1261 ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg
     1321 gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc
     1381 aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt
     1441 tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag
     1501 gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt
     1561 tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac
     1621 tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc
     1681 ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc
     1741 agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg
     1801 atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc
     1861 ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca
     1921 acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag
     1981 accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat
     2041 ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg
     2101 ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag
     2161 atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc ttagttaata
     2221 attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt
     2281 tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg
     2341 catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc
     2401 gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg
     2461 taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg
     2521 ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg
     2581 gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat
     2641 tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa
     2701 tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa
     2761 aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag
     2821 gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg
     2881 tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat
     2941 ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg
     3001 atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa
     3061 atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac
     3121 cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt
     3181 catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta
     3241 aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat
     3301 caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa
     3361 cgacttatta aaacaatact ttggttatga tgagtttaga cctggtcaaa aagaaattat
     3421 ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc
     3481 aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt
     3541 aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt
     3601 tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca
     3661 gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc
     3721 aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg
     3781 ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa
     3841 tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa
     3901 aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct
     3961 acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat
     4021 caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta
     4081 tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa
     4141 tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac
     4201 aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat
     4261 tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc
     4321 cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca
     4381 gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac
     4441 agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac
     4501 aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac
     4561 gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca
     4621 aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg
     4681 acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat
     4741 tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa
     4801 agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc
     4861 caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga
     4921 actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc
     4981 cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa
     5041 aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa
     5101 tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca
     5161 actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat
     5221 tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta
     5281 aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc
     5341 tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa
     5401 gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt
     5461 gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa
     5521 aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag
     5581 actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata
     5641 gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc
     5701 agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag
     5761 atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc
     5821 tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag
     5881 gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga
     5941 ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac
     6001 ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg
     6061 cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata
     6121 ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata
     6181 tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt
     6241 accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg
     6301 ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat
     6361 ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga
     6421 ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag
     6481 gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt
     6541 tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca
     6601 cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct
     6661 ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga
     6721 tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt
     6781 gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat
     6841 gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa
     6901 ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct
     6961 gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc
     7021 gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat
     7081 aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat
     7141 ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt
     7201 atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag
     7261 agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat
     7321 gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg
     7381 gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac
     7441 caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc
     7501 tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa
     7561 tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa
     7621 catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct
     7681 caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat
     7741 ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac
     7801 acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga
     7861 aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt
     7921 acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt
     7981 agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa
     8041 ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt
     8101 cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat
     8161 ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa
     8221 gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc
     8281 gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt
     8341 agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt
     8401 gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca
     8461 acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt
     8521 cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg
     8581 tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga
     8641 atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc
     8701 caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc
     8761 aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta
     8821 gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag
     8881 gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat
     8941 actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta
     9001 gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc
     9061 agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca
     9121 cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc
     9181 ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat
     9241 ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg
     9301 tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta
     9361 gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa
     9421 aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg
     9481 caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca
     9541 ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa
     9601 ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat
     9661 ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg
     9721 gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa
     9781 cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat
     9841 cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg
     9901 atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga
     9961 gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa
    10021 ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt
    10081 gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt
    10141 ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt
    10201 tgttttggct gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc
    10261 cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt
    10321 ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc
    10381 agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa
    10441 caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca
    10501 gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag
    10561 ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt
    10621 accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa
    10681 ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat
    10741 tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg
    10801 gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg
    10861 ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca
    10921 tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga
    10981 caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca
    11041 gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta
    11101 aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt
    11161 cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg
    11221 attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg
    11281 gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc
    11341 ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa
    11401 cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa
    11461 gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa
    11521 cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga
    11581 tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa
    11641 gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt
    11701 ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg
    11761 cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt
    11821 gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt
    11881 ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta
    11941 ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga
    12001 cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa
    12061 atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat
    12121 tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc
    12181 ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca
    12241 gaccaactac agataaagta aaagaatcca tattcaatat gattggacca tattttgatg
    12301 gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta
    12361 gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag
    12421 aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca
    12481 aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc
    12541 catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt
    12601 ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg
    12661 ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa
    12721 aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg
    12781 tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt
    12841 tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa
    12901 agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga
    12961 aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt
    13021 tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt
    13081 cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact
    13141 gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg
    13201 aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg
    13261 gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag
    13321 ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt
    13381 ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta
    13441 aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg
    13501 aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac
    13561 aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga
    13621 gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag
    13681 tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag
    13741 ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg
    13801 atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata
    13861 ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag
    13921 cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg
    13981 gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag
    14041 gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag
    14101 atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag
    14161 aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag
    14221 tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt
//
LOCUS       DARDHD010000065        13983 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000065 DARDHD010000000
VERSION     DARDHD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.065"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            259..2730
                     /locus_tag="SDA06_001480"
     CDS             259..2730
                     /locus_tag="SDA06_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4228760.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2833..4569)
                     /locus_tag="SDA06_001481"
     CDS             complement(2833..4569)
                     /locus_tag="SDA06_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG4228761.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4566..5270)
                     /locus_tag="SDA06_001482"
     CDS             complement(4566..5270)
                     /locus_tag="SDA06_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228762.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5401..5904
                     /locus_tag="SDA06_001483"
     CDS             5401..5904
                     /locus_tag="SDA06_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG4228763.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5864..6202
                     /locus_tag="SDA06_001484"
     CDS             5864..6202
                     /locus_tag="SDA06_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG4228764.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6223..7641
                     /gene="ffh"
                     /locus_tag="SDA06_001485"
     CDS             6223..7641
                     /gene="ffh"
                     /locus_tag="SDA06_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG4228765.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7753..8331
                     /locus_tag="SDA06_001486"
     CDS             7753..8331
                     /locus_tag="SDA06_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG4228766.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8335..8856
                     /locus_tag="SDA06_001487"
     CDS             8335..8856
                     /locus_tag="SDA06_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4228767.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8935..9489)
                     /locus_tag="SDA06_001488"
     CDS             complement(8935..9489)
                     /locus_tag="SDA06_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4228768.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9742..10017
                     /gene="rpsP"
                     /locus_tag="SDA06_001489"
     CDS             9742..10017
                     /gene="rpsP"
                     /locus_tag="SDA06_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG4228769.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10029..10280
                     /locus_tag="SDA06_001490"
     CDS             10029..10280
                     /locus_tag="SDA06_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG4228770.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10405..11196
                     /locus_tag="SDA06_001491"
     CDS             10405..11196
                     /locus_tag="SDA06_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228771.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11366..11887
                     /gene="rimM"
                     /locus_tag="SDA06_001492"
     CDS             11366..11887
                     /gene="rimM"
                     /locus_tag="SDA06_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG4228772.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11877..12638
                     /gene="trmD"
                     /locus_tag="SDA06_001493"
     CDS             11877..12638
                     /gene="trmD"
                     /locus_tag="SDA06_001493"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG4228773.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12774..13121
                     /gene="rplS"
                     /locus_tag="SDA06_001494"
     CDS             12774..13121
                     /gene="rplS"
                     /locus_tag="SDA06_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG4228774.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13253..>13983)
                     /locus_tag="SDA06_001495"
     CDS             complement(13253..>13983)
                     /locus_tag="SDA06_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4228775.1"
                     /translation="VSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKTIKIKD
                     KFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVI
                     NGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKG
                     TPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSD
                     ITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4585 a         2541 c         2853 g         4004 t
ORIGIN      
        1 ggtaggatga cgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa
       61 acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caccaacaca aaatattata
      121 gaaccctttt tttttgctat atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga
      181 acactttcca tatctgttct atactggata ggagaagttt ggaaatatcc agaaaacgaa
      241 atcaatcgag gtagccacat gaagaataaa tggataaatc gaagtaaaaa aaagaaagta
      301 acacaaagat catcagataa agtcatccgt ctttttccat cgtataaaga aggattaacg
      361 gcagatcaag tcaaggaacg aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca
      421 ttcaaaacaa atcagcaaat cgtattggaa aacatattta cgtatttcaa tttgatcttt
      481 cttatcttag ccattttgct ttgtttagta gaatcctata agaacttgac ttttttacca
      541 gttatcatag ccaatacagg aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg
      601 gacgaactaa atgttttaaa tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa
      661 acaatagata tagaagacct tgtaatagat gacattgtta ttttagagac tggatatcaa
      721 attccagcag atgcagaggt cgtggaaggg aaactgcaag ttaatgaggc tttattgaca
      781 ggcgaagcag atgaaataac caaagaaata ggtgatccat taatgtccgg cagtttcatt
      841 gtttccggaa aagcttacgc ccgattagac aaagttggag cagattcgta tatttcccag
      901 ctgacagtca aagccaagac aatgggagaa ggagaacagt cggagatgat tgcttctctt
      961 aatcaattga tcaaatggat tgggatcatc attattccta tcggtatcct gcttttttct
     1021 caaagttact tttttaatgg aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca
     1081 ttgatcggta tgatacctga aggactgtac ttgctgacga cgattgcatt agcattaagt
     1141 gcgactaaac tagcaaagca aagagttctt ttacacaata tgaaaagcat tgagacattg
     1201 gcaagggtaa atgtcctttg tgtagataaa acaggaacga tcacagaaaa taaaatgtct
     1261 gttcaaaaaa tgatcgtctc taaaaaacaa gaaataatca gtcagtcaga tcaattggaa
     1321 gcatgcattg ctgactatgc aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt
     1381 aaagcatatt ttacaaaaac cactgatcaa tcctatcacc atgttattcc tttttcttct
     1441 gtccaaaaat tcagcagtgt agcgttggca gacaaggtgt atgttttagg agcgcccgaa
     1501 atggttttgc gcgatcaatt gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa
     1561 ggctatcgcg tgttggtctt tggtgtgtac gatgggattc ttgaagaacc agtattgaca
     1621 gaagcagtca caccattagg ctatattctg attgctaatc ctatcagaaa agaagcaaga
     1681 gccacatttg attacttcaa aaaacagcaa gtagccatca aagtgatttc tggagataat
     1741 ccgctcaccg tatccaatgt agctgtccaa gcagggattt cacatgctga tcagtacgtt
     1801 gatgcatcta aattatcaga agaagaatat gaagcagctg tcgaaaaata tacagttttt
     1861 ggacgcgtca agccagaaca aaagaaaatc ttcgttcaat tgataaagcg taacaataca
     1921 gtagctatga caggtgatgg cgtgaatgat attctagcca tgaaagaagc agactgcagt
     1981 atagcgatgg cttccggaaa tgaagccgcc atgcaggcct cacaagtggt cttacttgat
     2041 tctgactttt cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt
     2101 gagcgatcag ccagcttgtt tttagtgaag aatattttct cctttttgct ctcactgttt
     2161 tcagtgatct ttgctctgac ctatccttta gaaccatcac aaatcacatt gattagtttg
     2221 tttactatcg gacttccatc tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga
     2281 gggaaattca tcatgaatgt gatggaaaaa gctgtgccag gtggactaac agatatgatc
     2341 gtagtcggtg cgttggtgat ctgcggggtg actttgaatc taaataaaac agatgtttct
     2401 acagcatcta ccatgctttt gattgcagta gggttcctcg ttctctataa aatctgttca
     2461 ccgctgaata aattcagaag tcagatcatt cttttctgtg ctagcggtaa ctttttttcg
     2521 gtcatctttt tgcataagct gttttcaatc accggtatct cagctgtatc acttttgctg
     2581 ttgagcattc tatttttctc ggcagattcg atttttaggc atttgaccac attggtagaa
     2641 tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa
     2701 tttctatcga tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag
     2761 aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa acgcacgagc
     2821 agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg
     2881 actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa tcgctaaccc
     2941 taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa
     3001 gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag aaaacgatag
     3061 atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat attggacagc
     3121 attttcaatt agatttttga taattggata aaggatatct gttttagtag taaatggttc
     3181 gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct gataattttg
     3241 aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag aattctcgcc
     3301 atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg catctttttg
     3361 catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat ccagtaaagt
     3421 ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga cgttgccaac
     3481 gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg ccacccctag
     3541 aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg
     3601 accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca cttgatgaat
     3661 cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag gttgatagct
     3721 taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag aatcagcatc
     3781 atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt gctcttctgt
     3841 ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa tcgtctgata
     3901 caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag gattttgcac
     3961 catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat agatcagata
     4021 atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga cccaacggcg
     4081 aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta aaatggcttc
     4141 taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat tcaaagtagt
     4201 acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga tttcgggacg
     4261 ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct ttccagtagc
     4321 atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag cagttttgtt
     4381 ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg ctttttttgt
     4441 caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca
     4501 gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat aaattttttt
     4561 ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt caccaagtac
     4621 tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg tacatctacg
     4681 attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg
     4741 atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt cggtgttagt
     4801 tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt gatttgtcca
     4861 atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc aattcgtctg
     4921 aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt caaatagtcg
     4981 tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc cgtcagcatc
     5041 aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat gccatccatc
     5101 gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc taattctaat
     5161 ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa attgaacgtc
     5221 aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat tcaaatctcc
     5281 tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag ggctatttta
     5341 caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga agagcagtca
     5401 atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa
     5461 gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt ctgtccagaa
     5521 gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg aaatggattc
     5581 gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg
     5641 tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat tgatctagtc
     5701 atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg aagtttttcc
     5761 ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca aaaaatgact
     5821 gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa tcgaaaagac
     5881 caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa
     5941 ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg aagaatacga
     6001 agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct tggaggatta
     6061 tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat tggaaaatat
     6121 gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat atgtacagca
     6181 aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt tgaaagctta
     6241 actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct
     6301 gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga tgttaacttg
     6361 caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg
     6421 gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt gacgatcaca
     6481 cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt tatcatgatg
     6541 gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa ttatttaaag
     6601 aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc agctgcgatc
     6661 gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat gggaactgaa
     6721 gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac
     6781 tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat ggatgagttg
     6841 aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga tgccatgact
     6901 ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat tacaggggtc
     6961 gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat ccgctcagtg
     7021 acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt agaagtattc
     7081 cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac attgattgaa
     7141 aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa
     7201 aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg tatgggccca
     7261 ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat cgaaaacgtc
     7321 aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat gacgccagca
     7381 gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc tgccggttct
     7441 ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg
     7501 atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat gtttggttca
     7561 ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa aaataagaag
     7621 aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg aaaatgtcta
     7681 ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac tctaaacgaa
     7741 gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg gcaatatcat
     7801 gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa attgatgctt
     7861 gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga agcattcgat
     7921 gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt
     7981 gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc agccgtccaa
     8041 aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga gtatctttgg
     8101 tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga agtcccaaca
     8161 acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt taaatttatc
     8221 ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga tcaccttgac
     8281 agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata aagcatgcga
     8341 aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa aacggatgat
     8401 cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc tttttatgaa
     8461 acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa atatttggag
     8521 accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc acaattacaa
     8581 ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg
     8641 acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa
     8701 catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg ggacgggatt
     8761 cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac gacacgattc
     8821 ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata aaaaaggcaa
     8881 ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg ctttttactc
     8941 tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga aacttaatgt
     9001 tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga tatatgcttc
     9061 cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc
     9121 gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt tatgatccca
     9181 aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata ctgctggcac
     9241 agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca cttcctcacg
     9301 aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat tgtaaaatgg
     9361 cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt tcgcaaaagc
     9421 ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa atccaatttt
     9481 ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa aagataagta
     9541 actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat tacttatttt
     9601 taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt aatcaagctg
     9661 taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt gtgaataaaa
     9721 caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat gggttctaaa
     9781 aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc
     9841 atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt tttaaaagaa
     9901 gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt acgtaacatc
     9961 ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa gaaataaggt
    10021 gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg ctagtcactt
    10081 atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag tacaatctaa
    10141 cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa
    10201 tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca
    10261 tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt
    10321 gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt
    10381 ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta
    10441 ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt
    10501 atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc caccactgaa
    10561 caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa
    10621 cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca
    10681 gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg
    10741 ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg
    10801 tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga cacaaatggt
    10861 cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt
    10921 tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc
    10981 caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca
    11041 gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta
    11101 gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct
    11161 gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca
    11221 tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt
    11281 ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca
    11341 gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa
    11401 cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg
    11461 ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg tggaattaac
    11521 agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc
    11581 tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt
    11641 agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga
    11701 tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt
    11761 cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga
    11821 agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga
    11881 agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca
    11941 tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt
    12001 cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc
    12061 tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca
    12121 aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag
    12181 aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac
    12241 gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag
    12301 aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga
    12361 atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata
    12421 ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata
    12481 aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg
    12541 atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga
    12601 aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tcttctttac
    12661 agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat
    12721 attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc
    12781 cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg
    12841 gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat
    12901 ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta
    12961 aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa
    13021 aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat
    13081 tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta tcagactgag
    13141 aaagaaccct tggtattcaa gggttctttt tttgttctct gtttttttat attaacgatt
    13201 tctatttggg acatttttga ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt
    13261 tttctatatc ttctttcaat ttttctttta ctccctttgt cacatgcaaa taaatctttt
    13321 cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga ggagttccta
    13381 gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt tttttatcaa
    13441 tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt tggaaaggag
    13501 ttcctttcgt tgtttgaaat agaaattgac cattcggatt taattctaga agctcattgt
    13561 aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca gtttttgtag
    13621 aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc ccatttataa
    13681 ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg attgcttctc
    13741 caggcctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt ttattgtgtg
    13801 aagttgtgaa atttactagt cttttatatt catcatcttc taaaaactta tctttaattt
    13861 ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga tttttttcta
    13921 catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta attacactga
    13981 cgt
//
LOCUS       DARDHD010000066        13831 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000066 DARDHD010000000
VERSION     DARDHD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.066"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            360..1799
                     /locus_tag="SDA06_001496"
     CDS             360..1799
                     /locus_tag="SDA06_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228776.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2094..2312
                     /locus_tag="SDA06_001497"
                     /pseudo
     CDS             2094..2312
                     /locus_tag="SDA06_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2561..2776
                     /locus_tag="SDA06_001498"
     CDS             2561..2776
                     /locus_tag="SDA06_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228777.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2993..3249)
                     /locus_tag="SDA06_001499"
                     /pseudo
     CDS             complement(2993..3249)
                     /locus_tag="SDA06_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3436..4347)
                     /locus_tag="SDA06_001500"
     CDS             complement(3436..4347)
                     /locus_tag="SDA06_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4228778.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4366..5370)
                     /locus_tag="SDA06_001501"
     CDS             complement(4366..5370)
                     /locus_tag="SDA06_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4228779.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5367..7490)
                     /locus_tag="SDA06_001502"
     CDS             complement(5367..7490)
                     /locus_tag="SDA06_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4228780.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7495..9942)
                     /locus_tag="SDA06_001503"
     CDS             complement(7495..9942)
                     /locus_tag="SDA06_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4228781.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9929..10318)
                     /locus_tag="SDA06_001504"
     CDS             complement(9929..10318)
                     /locus_tag="SDA06_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4228782.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10378..10881)
                     /locus_tag="SDA06_001505"
     CDS             complement(10378..10881)
                     /locus_tag="SDA06_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4228783.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10894..11118)
                     /locus_tag="SDA06_001506"
     CDS             complement(10894..11118)
                     /locus_tag="SDA06_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228784.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11222..13132)
                     /gene="ltrA"
                     /locus_tag="SDA06_001507"
     CDS             complement(11222..13132)
                     /gene="ltrA"
                     /locus_tag="SDA06_001507"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG4228785.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         3919 a         2889 c         2423 g         4600 t
ORIGIN      
        1 tatcatagca atgtatttga aaagcaaatt ggaaaacaaa taggtgcaac accataaata
       61 tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg tctcacgaga tagctcacac
      121 tattaatctg aagaacaatg aagtcgactt ttagaaattg ctagaagaaa gtaaagcgaa
      181 ttatcactag ttactataga gtaagttcaa ataaagcatc tccaacttac atggttacat
      241 gaggatatca aatcgaaatt actcaagctt cctatggcta gagtcgcgag aagttgttgg
      301 gcagtagaca taggttatat tgttcttagc tacacgatca ttagaaagga aatcctatta
      361 tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg ctggtggatt ttgattgcta
      421 tatctatgtt taccttcatg tccacattgg atggcagtat tgtcaatatt gcactaccta
      481 ccatctcaga tgatatgggc ataccaatga atcaagcgga atgggtagtg tccgtttatc
      541 tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat tggcgatagc cgtgggaaaa
      601 tcaaagtatt tcggattggt acgctgatct ttgtcttggg atcgctgttg tgtgggttca
      661 atcattcgct aggtatttta ttgttcgcac gggtggtaca agccattggt gctagtatga
      721 ctatggctac aaatgccgga atcatcacgg aagtctttcc catacatgaa cgtggtcggg
      781 cattgggagc aataggtgcg tttgtttcat taggctcgat tgctggacca ggtcttggcg
      841 gcttgatttt gtcccaattt tcttgggcgt acattttttg gatcaatgta ccggtgggaa
      901 tcgtaacgat gttgattgct gaaaaattct taccaaaaga tatcaccatg agcggccata
      961 caatcgatgg agggggcttt gctgccattg cggctacgat tatcagcttt tttggagcaa
     1021 tctttttggg gcaagaaagt ggctttttcc atccagtccc ccttgtactg ttcgtaattt
     1081 cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa agcagcctct ccattgattt
     1141 ctttcggtat ttttaagaac aagattttta ccatgagctt gattacagcc ttgttgattt
     1201 tttcttccaa cttctttgtc aatgtagtga ttccttttta tctgcaaaat gctcgcgggt
     1261 tgagtcctag ctatgctggg ctgttgatga tggtctttcc tttgatgatg gtcgtcgggg
     1321 cgcctttaag cggctacctg accgaccggg ttggcccgga gattttggta ttagtaggct
     1381 tgggcttatt gtcggtaacc caggtcatgt atatgttttt gtcagtggac tctcctttgt
     1441 ggctatacat tgtcgctact gccatcatgg gtgccggcaa tgccttgttc cagtccccca
     1501 ataatacgat ggttatgagc tcggtcacta tcgaaaactt gggactggca ggctcgatga
     1561 attcctttgc ccgaaatttg gggatggtca gcgggatttc gctttctacg acggtcttat
     1621 atcaagcgat gtctgctaag atgggccagc aagtaactaa ctatatcgca aatcggccgg
     1681 atgtcttttt atatggaatg aagattacct ttttaggcag ctttgttttg tgttttgttg
     1741 cctttttcct gactatctgg cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa
     1801 aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg tgagtgggat ttccatggat
     1861 gatggaagat gaagtgaact tctaaaagcc gaaaaactca agtattatct tcaaaatact
     1921 agtgctcaat gagatgcttg atttgatcac aagaagactg cgaaagccgc tggcaaccgc
     1981 aagctaggta tcccaccgat ggactacgtt ttagaggcta ctggttatga atacggagcc
     2041 aacgctctca tctgcataag aatacatcac ccagattatg taaagatttt cgatacttct
     2101 gtcaaaaaat cattaagata gataaatgat ttccgtctag tcaaattggt tcagaatgta
     2161 gacacacaga tggtaagaag tcactcacaa ttcgagaatg gacttgtcct atttgccgta
     2221 tacatcataa tcgaaacgtc aacgatagta tcaatatttt gaccaagggc tatgaataca
     2281 agcattgcct tagaaaacat caagaacctt aggaacaacg gggatagctt ggttaataag
     2341 agataccgct gtttaggctt gcaagtatac tcttttatca agaattccgt ggctttagtc
     2401 atgagaggct taatactatg aaaattttct ttagcatttt tgatttaggt tccttttcta
     2461 attataataa gatgaccact acagtaacct actgaaatat ataaacagcc actgtagtac
     2521 cattttcaaa gtagtctatg agaggagaat cttcattctt atgaacgctg gaattgcgga
     2581 tatgaattta ataaaaaaaa cccttaatga ttttacttcc aattcaatat caaaaggtac
     2641 tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt gaaagatcag tggaaaagct
     2701 gaatctttta gatgctataa aaataacgga atttgctatg aaaaatggaa aagctgagat
     2761 tgaaatatgg agataattat cgtctagtga attacttgcc acttagctga atttaacgat
     2821 ccttggtgct tgaaggggga gattcttggg aatagagcat acttgcaagc ctatgtcttt
     2881 actaacaacg atatctctta ttaaccaagc tatccccgta gttctaaggc ttttactttt
     2941 atctaagcaa taatcgtatt caaaggaaat ttttgaccct tagaaagtct cattttatca
     3001 gacgtgagct taggcattgc tgaattttgt taattcggtt ttaggttgtg ctttgagcat
     3061 aatatgaaag tattctttat cacgatttca ttcatctaag gtaatgtgat gtgattctga
     3121 aattctttca aaggcagttt ttgcataagc tgacattaca tcatcaatga catgtttaca
     3181 atgtttcact actaaaataa gggggtaacc aagaagaaaa actgaatgat tatttgtatc
     3241 taattccatg gtatatcaac catttcctat atagactgat tatgacatgg attaaaataa
     3301 aaaggcacta tgtcttaatt gtcggttttc gagttaccga atggcattca tcacccacct
     3361 atagaggatg ggcgacttct gcctgaaata agttacacta cttataactt ctaataaaat
     3421 tagattgagt cattattatt taacatcaac gattttccag tttccatttt tttgtaatac
     3481 taatttgtac tgaaagtagc tagtggtttt tgatacatta tcaaggtatt ttatctttgt
     3541 actcactaga aaattttttc cgttttgtag ataaactgga ttctctaact ccgaaaactt
     3601 taagtcaata ttgattggtc gcatcgcacc attttcaaca taatactcca attctttttc
     3661 agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa tcggtgattt cttccgttgt
     3721 tttggcatca atttctgaat cgttttctac ttgttttgct tcatattttg ccttgtctgg
     3781 ctttccaatc attgttggca aactagttac taaatgattc ctgttttcat cttcaaagat
     3841 tgtcactcga tatgcagaag aaattgattg cgtctttttt ccctctgtaa tatgttgctc
     3901 cactaaaaat gaaacgacaa attcttccga gtgtttttca acatctagaa tttttacaga
     3961 ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata tcttggatta aagcgagacc
     4021 gttttctgta aggtactgtt cgagattact cattctcttt tcaattgctt ctttgttatt
     4081 tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa ctttcaacgc cagaagtatt
     4141 cactaccttc tcttttataa ccactttttc atgagttgta tgttgatcaa tggcggtgaa
     4201 atttttataa acgccaaaag caagactaca agacaataaa agccaaaggg caagcaccat
     4261 ttttcgatgc ttacccacgg atacaactcg ttcttttttc ttttttgggc tcttttcctt
     4321 ttgattccgt tcaattttta tcttcattct ttttcctctt ttctcttatt tgtatcggcc
     4381 agctccgata agatgctgcc gccaatagtt ttccgttaga ttcgtccacc ccactggatc
     4441 tcctgcatga aacatgcgat tctcccctgc gtaaatgccc acatgggtca cataagtacc
     4501 ggcatcatag gttgaatgaa agaaaactaa atcacctggt ttggcatctt ttagatcaat
     4561 atgagtcatg gcatcatatt gatcctgagc cacccgagga agtttcaggt caactttggc
     4621 ataacaccat tgggtcaagc cactacaatc aaaccccgtt tctggacttg agccaccaaa
     4681 cacataggcc gttccttcat acttaaatgc ctcatcaaaa atttcttgga ctattttgtc
     4741 atcaaactga gttgtagtta ggtattgctt cactaataaa cagtaaaaca tattgccata
     4801 attataccgc cacccaccat tttccttaat cgcgattggg ttggggtaat cgactttttg
     4861 accacctgat ttttcctttg aaaaagattc agctaaagca aaactatgct tcttcccgtt
     4921 tttcccgaca tgatctagat acccgccacc atagttatag gcctgaataa tactatcttg
     4981 atcacaccct agttctttgg aacgcttaac caaagacgca aaatagagac agccttgttt
     5041 gatggatgcc tcggtactta atgaattagg tggtaatccg gctgactcag aactttgcat
     5101 aacgtcttcc attgtgccac cagattccac ttgaataatc gccaaaagaa ttggaatata
     5161 ctctgttata tccgattctt tggcatactt ttccaccatt gcacggtgtt tcaatacttc
     5221 ttctgagaca ctagttccgc caggagaagt gccagaacta ccatttccct cactgtcttc
     5281 accaaacaac aaacccacac acatcaaaat acccataaaa gagatggtca gaaaagttaa
     5341 tgaaataaca cccactttct tcaagttcat tttttctcac ctgaccttga ttttggacga
     5401 tggttcattc ttttttgtac tttttgattg gtctgctttg gcagagtgga cctctgtttc
     5461 acttgttgga aagaattttt cttacttgct gattggcttt tcccttttga aatgatttct
     5521 tgactagtta gttttcgttt gatttcggga ttcagatttg ctctcacaat ccgctttggt
     5581 gcttcttttt tcagcatcat atctttctga ggttgagctg ctttatctat tggaatattc
     5641 gcccttcttt ttccctgacg atttgttgct tcactcagta tcttgcgttt ttcgtccatt
     5701 ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca ctcgatccgc tttttcttgg
     5761 aacagtccgc gcttgaattc ttctggtgct tttttcgact tttcgattcc tttatggaga
     5821 ttgtatttca aattcgttgg cgtattcctt acctgatcct ttacttgttt agtcttatct
     5881 ttcaccaaag cttgggtatc caaaactttg ccgatttttc tcccagccaa attcattcgg
     5941 gattgtttct tattatttga aactggattc tccttagtta agtcagatag tatttcgtga
     6001 tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg atggcttaaa tttggatttt
     6061 gattgttcct ttgccaactt atttccgact gctgcaccac caccaaaagc caaagctcgt
     6121 cccacatttc gattgagttg ccgcaatttt cgattcatta acatttgcgg ttttcgcatg
     6181 acgcgtcttc ccattgactg gctatcatta ctctggaggt tgaacatact cataatgtcg
     6241 cccaatttga aatagatacc ggcaaaggtc acaatttgca gaaaagcaac catgaaaaat
     6301 ggataggttg ccgaaatatt gaaaaacatc gtggagatgc taaatgcagt cgtaatcacc
     6361 aaagtaacac ccgctcgcat cataatcgta ttgaataaac gaataatcgc cttctttcct
     6421 aaactttcat aagtcggcaa catggacaat aagaaactaa ttggcagaaa catcgcaaag
     6481 ataatgaaga gaatttgcga gaacaacata atccctgtta gaagaaaaac aaagaaggaa
     6541 atgccaatat tgaacaatac caaaaagacc acggtcccta aacggttcat ggtcttggta
     6601 atactgagat tcgcattgtc attgtcttca atctctgcct tgaccgcttc ttcccgatct
     6661 ttgcctttgt tttcatctgg actcacagat agaatcttgt tgacccggtc ttcaccaatt
     6721 tcttctatgt tggaatcatc aaattgcagt agtaaccaag gttgttggac ttgaatcgaa
     6781 aacaaactat cccgaatcaa atccacacta tcttttcctt ggctttctga attgggtaca
     6841 acgatctcgg ttccaagggt aagggccgct tcactaacat ctgagctaaa gtcattgatt
     6901 tttgtgatat aggtaggcgc ataagcaatg aacgaacccg atagtagaaa aatcatcaca
     6961 aaattaatca cggctcgaac tgctttcgtg gtttctcgtt ttaataatcc ggtatacgcc
     7021 acatacaccc ccagagctaa aatcatcaat aatagaaacc caaaataaaa tccggaggtc
     7081 tggagtccat tttcagtaat accagccaat gtttggatat tctttccaat actttcagcc
     7141 gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca cataacccgt ggcgttcgac
     7201 aaatacaaac tcaccgtcca aatgaaatta gtaatggcat aaagaccata catcacgctt
     7261 ttccctaagc catcacccca attccaaggc aaccaatccc aagagctatc cacaaagaaa
     7321 tctagttgat agttgttcag tgaatatttg gaatatagat ttcctgcttc aactgtatca
     7381 tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac tcaatagaac tatcagagtg
     7441 agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt tctttttcat cccctcactc
     7501 cattctctct tgaacaggtg gacgggtatc aaaggcatgg aagagttctt caaagactgg
     7561 atgtacctga accacgccta cccgaccata caaatcctgc atcaagcatt ggccattttc
     7621 caaatctcgt aggcgttttt gattgctttc atcttctcga tccaagccaa agaattccaa
     7681 ggttttcttg atttcatgaa tatcggtact gcgaaacgca aacttcaacc cgatattatt
     7741 cttcaagcgt tcatccagta aatcatcact attttgcgtg acaaagtaaa ccccggcatt
     7801 catagcccgc ccagcacgaa cgagcttatt cgataatgtt ttcccttgcg ctacttgaag
     7861 aaaactccac gcctcatcta agtctacaat tttgaagatt tctcgattac tatggataaa
     7921 gtccaaggca aaagtggaaa tgacgattag catggaaaca ctaagcaatt ccatggtggt
     7981 atattcttca aaagtggttt ctgcatctgg taaaaccaaa tccgctactt gaatgatatt
     8041 taactgccta tctaaactaa tcgactgggt aatcgaacca tctgagaaaa gcaaatgggc
     8101 aaaatcataa tcggtaaagg actcgatgtg atccgcgata ttttccgcaa ctggtgagcc
     8161 ttcttttcga agctcattaa ttacttgtaa taatccccgt tgattgcttt gggtaaccgt
     8221 ccgaatggcc ttacgcaaca ctggaaactt ctccccatct cgactagaaa ttcctgttaa
     8281 aaaggtcaga atatcaattg ctagactttc agaatccttc tttttctcca taatcacata
     8341 aggatcaagt aatccttgat tttttggctt actagttaag ttgagaatat tgatttcatc
     8401 cgcaatttct ggcaaggttt ctttccagcc accccgttcc ccttttggat cgacaatcac
     8461 agcttgtcca ccaaaaagga cggcgtaata aactaagaga ttgttactaa aggactttcc
     8521 gccaccgagt gaaccgagga atgccgctgc tagcgcattg gtaactgaat ccttgacccc
     8581 ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc gtatctacgt tataaccaaa
     8641 gtaaatgcct tccaactccc ctaacatctg agtggcgcca aaaccaagtc cggcgagaaa
     8701 atcagacgta acatactgga tataatcatt catgtatcgc ttactagaag gtaagaattc
     8761 ttcatgtagc cccatcatat cgccaaaagg ccggactagt tttacattgg tatcatcata
     8821 aaaatctagc acttcatcac accggcgctt tagttcatct actgattcgg cacttactcg
     8881 aaccacatag ctcagtttgt acatggattc tttggactga tccaaggtag tttccaattc
     8941 atctacggaa tccaatgcat ctaagacatt ggaatttgtt tcattgtcag attgataggc
     9001 atggttgtcc aagtctttta attccttttt cttattgcga acggtagcaa gtgccttttt
     9061 gttcgtcaca atctccacat tcatactagt gtcaattggg aatgtaaatt gttgttgctg
     9121 gtaatagaaa agttctgagg agggaaactc catttccccc acaattgtat taattgttaa
     9181 gtacgctaca tatgattcat gattctcatg ttccatgcat aagtaccggg gcttttcttc
     9241 aatcaaacaa cgactgggac gtaataagtc gtaacgtttc accaaagttt ccgacttcag
     9301 ctttttcttt ggtagctgaa attcatattc ttcaaatggt gtaccctttt cgccatagat
     9361 atgttcaatc aaatacatca gatcactcgg tgtgactcgt ctgactttaa atcttcgagc
     9421 caatttactt tccattagtt tttcaagctt ggtatatcgg cgaatttctt cattggataa
     9481 ggaaacaaaa tcgcccatca aatgatgatt cactccatag acaaattctt gaaagccgga
     9541 gaagaaagat ttcttgagat tctttagatt gacctcttca tctgtcgcaa tcagtttgaa
     9601 gccaataaaa aagcgataat caatctgact atcgccaatc atggatacta atgcctctgt
     9661 ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc ccggtaattt ccttttttga
     9721 tcgttcttgc gtggcacgaa tactactttc tgtggcaatt tgtaaggcat gaatcttgcc
     9781 ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg acttgatatt tctgttcagg
     9841 tgacaaaaag gagtaattgt aaggtattaa ctcatagtag gcaaaacatt ctccctcttg
     9901 attaaagaca agattatttt caatatactt aatcgggtac atagttcaca ctccttacca
     9961 gagtaatggg ttcgtcccat cgtttcttct gttctttgac tttctttcct gcgaaagtga
    10021 ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa tcccaatggt ttcttaccgt
    10081 caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg tatacctaag tattttaaaa
    10141 aggccccctc tatcatagat agtggaggca catgggcaaa tacgatgaca aacagcaagg
    10201 ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa aggcagttga aagtcattaa
    10261 ttgcataaat gaccttttca actgcccaaa tactggtgta gctttttatt ttcttcatca
    10321 aatcattcct ttaaaaagaa ggatagttta gaaatcaact acccttcaat aattttgtta
    10381 atatggatac tcaaaaacac ctcgactcgt taccaaataa ttgcctccaa tctccaagtc
    10441 tctaccacac gcatcaaaat caatatacgt cttcaagtgt tctggtactt ctccaaaatc
    10501 accagcttct acaatcaaga aatatgctac atcactcata tcatcgcaat ctggataata
    10561 gataatatca tccacatgct ctaccatctc ctcaaaagaa ttgaagaaag catgttgaat
    10621 ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc tccgctaagc cacacaaccg
    10681 attaatttcc tcaattggcg tatactcatc aatctcaaag ggaagttcat aatcatgaat
    10741 tgcatattcc tcgtattcgt catttaaccc aattcgttct ttcacttcat caaaatcgac
    10801 aggtggggta aaccacgccc ccaccaattc accttcgtta tatttcccga ggttcgcaat
    10861 ataaacacgc atctgttcca tcgtgcttcc cccttacgca ccgacaattc ggttaaacaa
    10921 actgagtaag acatccttca ccccagaagc attaaagacc aatccgaccg caatcaaagc
    10981 aatcaccaaa aagccaatca gtttggaaaa ctcccgctta aatcctaaaa acaaaccaat
    11041 gaccacaatt gccagtaaga ctaaggattg ggcattggtt aaaaaccatt gatatatgga
    11101 taggtaagtt cctgatatta taaatatctc aaacttttcc ccccattcac acggagcgtg
    11161 cgactttcac cgcactccgc gttccatcgt gtttatattg aaaagtattt atgtagtaga
    11221 cctatatcaa gtttaattct tgattgccag ctaatactct atatttattt agtcttatga
    11281 gctggacttt atttagcttc aatagagcta tatatttttg tattgtttct tctgttttag
    11341 cgtgtattag acggtgtacc gcctctttta ccaaaataag atttttataa tcgtcatttc
    11401 caccagctcc agttggtaca atatgatgac aatgtatttc attagcttgt tgaaattctt
    11461 ccagagttat agcacatttt ccatgttgag cagaaaatag cgaaattcga ttgtctgcgt
    11521 attctattgt gcgattttcc aaagataatt tcattaactg gtacatcagt aatttatcaa
    11581 cacttagatt ggtgtggata aattttcttc cctctagagt gtagcaattc gctaaataca
    11641 cctttgccat tggttttctg tgttggatat atccaacagg gaaaataggt tcgccctcgc
    11701 cagcaacata ccgaagcatt ttagatttac catatcttgt tgcctccttt gtcgtaagtt
    11761 gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc taatctgtta gtgaagatag
    11821 ccattacagc tctattcagt ttcatacaat ccagatttac attcgttgca atctgataat
    11881 aattttgcat acctaatacc attgaattat aaagtcttgt ttcttctaat tccgttttcc
    11941 cttttgaggg agaagcaatt ttctttgcct gtgataccag cttgtctttt tggtttttga
    12001 attgtttatc acttatatgt gatttaacaa ctaacttctt tcttctcggt atcattttaa
    12061 tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt tacaattctt gttttttctt
    12121 gtgatacttc caattttaat cgttgttcaa tccattgagt gattgctatt ttggcttttt
    12181 cagcactttc cttgtagcgg cagaaaactc taaaatcatc tgcataacga accatataca
    12241 tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt gcttttacat gggctaccgc
    12301 ttttattgat atgaaccaca tagttttttg taacaggatt ttcctgccat tggctttcta
    12361 cccaatggtc taactcatta agaacaatgt ttgctagtag tggggatatt attccccctt
    12421 gcggagttcc tttctctgga tatgttattg taccgttagt cattttgatt ggagctttta
    12481 atatcctttt taaaatgaat atcaagtgct tgtcccgtat tcccatagcc catatctgtt
    12541 ttatcagctt ggaatggttc acgttgtcaa agaaaccctt aatatcaaat tcaatcacat
    12601 aatgtagctg gctgatttgt aggcggttat aggttgcttt tattgcattt tcaactgacc
    12661 tattaggtct aaaaccatag ctgttttcac taaatttagc ttcgcagatt ggttctaata
    12721 cttgttttat acattgttgt ataagcctgt cccacataca aggaatacct agaggtctgg
    12781 ttttaccatt tggttttggt atttcttttc gcctcactgg cttaggtcga tacccatgtg
    12841 gactgcctgc aacaatgtat cttacttttt tgacaactgt ttctgctggc aatttgccaa
    12901 tatctttaat tattgtaccg tctgttcctg ctgttttact gcctgcattt gctttgatat
    12961 ttcgatatgc cagtaatata ttttctctcg ataggataat atccataaga tgagtgaaaa
    13021 tatctccatt cttacttttg gcatataaat catcaaaggt gttttgcatt tcgtaatatt
    13081 cagcgtgtct taaatcttct acacacaatg tttcattttt atttttaggc ataaggcttc
    13141 actcctttac ttgaaattga gtgagctttt ttatcttact cgtaatcctt attgaaacaa
    13201 cataatactt ttctaatata tacacgactt gagggcattc ctccattctc attacaagaa
    13261 tttcttaggt tcgccctctg cttttcagca ttagttcaac tgcttattgt tcttcgtcag
    13321 tttattctct tgatgagaac ccaatacctt tcctcgttcc gataatctta tctatacata
    13381 tatatcctta ggtcactagc tataagcctg ctggcttgat accgcctgta acgttatatg
    13441 gcatttcata acctatattc ttactttgcc acaccagcta cgatttctcg tacctttgca
    13501 tttctacaaa gttcaaattt agacccgtac attcgctgtt tcgtcagttc cttatagaac
    13561 attctaacca tagatatttt atagaccccc gacctgttgg atacgctacc cacctctgga
    13621 tagttttcga tagaagatat atgttcatca actattacgg tatctttcag ccgtctttac
    13681 cgagcttcaa acattctgag tttcctcata caatgcttgt cggaggctca gggcaggcgt
    13741 ttcagagcgt tacttcatca ttccacctgt cagattatca gttctcctaa taaccaaact
    13801 ttttcgttct tttattagtg ccttagcttt t
//
LOCUS       DARDHD010000067        13751 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000067 DARDHD010000000
VERSION     DARDHD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.067"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            168..506
                     /locus_tag="SDA06_001508"
     CDS             168..506
                     /locus_tag="SDA06_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228786.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(711..1286)
                     /locus_tag="SDA06_001509"
     CDS             complement(711..1286)
                     /locus_tag="SDA06_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG4228787.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2018..2599)
                     /locus_tag="SDA06_001510"
     CDS             complement(2018..2599)
                     /locus_tag="SDA06_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4228788.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2782..3408)
                     /locus_tag="SDA06_001511"
     CDS             complement(2782..3408)
                     /locus_tag="SDA06_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4228789.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3430..4761)
                     /locus_tag="SDA06_001512"
     CDS             complement(3430..4761)
                     /locus_tag="SDA06_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG4228790.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4916..5317)
                     /locus_tag="SDA06_001513"
     CDS             complement(4916..5317)
                     /locus_tag="SDA06_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4228791.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5403..5609)
                     /locus_tag="SDA06_001514"
     CDS             complement(5403..5609)
                     /locus_tag="SDA06_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228792.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5704..7206)
                     /locus_tag="SDA06_001515"
     CDS             complement(5704..7206)
                     /locus_tag="SDA06_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG4228793.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7224..7679)
                     /gene="argR"
                     /locus_tag="SDA06_001516"
     CDS             complement(7224..7679)
                     /gene="argR"
                     /locus_tag="SDA06_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4228794.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7777..8394)
                     /locus_tag="SDA06_001517"
     CDS             complement(7777..8394)
                     /locus_tag="SDA06_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG4228795.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8615..9988
                     /locus_tag="SDA06_001518"
     CDS             8615..9988
                     /locus_tag="SDA06_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4228796.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10063..11175
                     /locus_tag="SDA06_001519"
     CDS             10063..11175
                     /locus_tag="SDA06_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG4228797.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11247..12884)
                     /locus_tag="SDA06_001520"
     CDS             complement(11247..12884)
                     /locus_tag="SDA06_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG4228798.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13052..13660)
                     /locus_tag="SDA06_001521"
     CDS             complement(13052..13660)
                     /locus_tag="SDA06_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4228799.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3717 a         2892 c         2460 g         4682 t
ORIGIN      
        1 tctgtctaac ttaatttgac aattaagaaa ctaaaaaaat aatccaagaa acattgaacc
       61 tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc aattttgcag
      121 ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg cccgattata
      181 aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag ttcacgaaac
      241 tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa atagaaacat
      301 tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag atcgtagcta
      361 tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat aaatggcaac
      421 ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact ttgaatttta
      481 gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat aaaaaagact
      541 cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact gtgaccctat
      601 agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt gggatctgtg
      661 ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg ttataccttc
      721 tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga aatatctgtt
      781 acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg gattggtgcg
      841 actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc atcctgatgg
      901 ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat aaatttgtct
      961 ttttcttcat tccactcata aaagccactc attgggaaaa cacaacgatt ttgcaaaaat
     1021 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
     1081 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
     1141 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
     1201 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
     1261 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
     1321 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
     1381 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
     1441 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
     1501 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
     1561 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac
     1621 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     1681 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
     1741 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
     1801 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
     1861 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
     1921 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
     1981 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
     2041 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
     2101 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
     2161 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
     2221 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
     2281 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
     2341 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
     2401 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
     2461 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
     2521 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
     2581 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
     2641 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
     2701 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
     2761 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
     2821 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
     2881 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
     2941 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
     3001 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
     3061 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
     3121 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
     3181 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
     3241 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
     3301 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
     3361 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
     3421 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
     3481 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
     3541 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
     3601 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
     3661 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
     3721 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
     3781 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
     3841 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
     3901 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
     3961 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
     4021 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
     4081 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
     4141 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
     4201 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
     4261 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
     4321 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
     4381 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
     4441 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
     4501 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
     4561 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
     4621 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
     4681 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
     4741 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
     4801 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
     4861 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
     4921 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
     4981 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
     5041 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
     5101 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
     5161 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
     5221 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
     5281 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
     5341 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
     5401 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
     5461 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
     5521 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
     5581 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
     5641 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
     5701 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
     5761 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
     5821 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
     5881 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
     5941 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
     6001 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
     6061 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
     6121 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
     6181 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
     6241 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
     6301 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
     6361 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
     6421 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
     6481 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
     6541 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
     6601 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
     6661 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
     6721 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
     6781 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
     6841 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
     6901 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
     6961 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
     7021 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
     7081 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
     7141 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
     7201 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
     7261 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
     7321 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
     7381 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
     7441 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
     7501 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
     7561 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
     7621 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
     7681 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
     7741 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
     7801 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
     7861 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
     7921 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
     7981 catctttttg aatactagtt gttctttcaa gtcttccgga agtttgctag aacgctgaat
     8041 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
     8101 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
     8161 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
     8221 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
     8281 attcgggtcg ccgatccctc tgatgctaaa gaatggtttt ttaggaacct ctagtatttg
     8341 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
     8401 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
     8461 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
     8521 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
     8581 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
     8641 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
     8701 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
     8761 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
     8821 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
     8881 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
     8941 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
     9001 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
     9061 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
     9121 gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat
     9181 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
     9241 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
     9301 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
     9361 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
     9421 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
     9481 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
     9541 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
     9601 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
     9661 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
     9721 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
     9781 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
     9841 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
     9901 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
     9961 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    10021 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    10081 atcggtggcg ggatcattgg tatgactcta gccagttact tagatacatc aaaattcaat
    10141 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    10201 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    10261 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    10321 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    10381 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    10441 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    10501 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    10561 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    10621 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    10681 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    10741 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    10801 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    10861 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    10921 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    10981 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    11041 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    11101 ttaaatactg gtcattggtc tacggaagat tatcaaaagg acatctccac ctatattacg
    11161 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    11221 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    11281 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    11341 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    11401 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    11461 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    11521 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    11581 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    11641 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    11701 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    11761 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    11821 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    11881 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    11941 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    12001 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    12061 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    12121 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    12181 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    12241 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    12301 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    12361 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    12421 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    12481 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    12541 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    12601 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    12661 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    12721 atcgttcctg gactcccttt tgctagttga taagctgtct tcctgtcaaa aacttcatga
    12781 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    12841 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    12901 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    12961 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    13021 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    13081 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    13141 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    13201 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    13261 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    13321 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    13381 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    13441 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    13501 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    13561 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    13621 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    13681 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    13741 aaacatgaca t
//
LOCUS       DARDHD010000068        13693 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000068 DARDHD010000000
VERSION     DARDHD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.068"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..921
                     /locus_tag="SDA06_001522"
     CDS             <1..921
                     /locus_tag="SDA06_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4228800.1"
                     /translation="TLLVSSESNIKNSSVVYVYGDEREYIESSEHKYDFKIGNILTFD
                     DRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKLIIFTKQKLKF
                     IPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDSEISTSGSLKP
                     TFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIIDDLYDTSDTMD
                     RIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGFWGINVHVPFE
                     KSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1439..1894
                     /locus_tag="SDA06_001523"
     CDS             1439..1894
                     /locus_tag="SDA06_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4228801.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2235..2837
                     /locus_tag="SDA06_001524"
     CDS             2235..2837
                     /locus_tag="SDA06_001524"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228802.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            2931..3263
                     /locus_tag="SDA06_001525"
     CDS             2931..3263
                     /locus_tag="SDA06_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG4228803.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3414..4393)
                     /locus_tag="SDA06_001526"
                     /pseudo
     CDS             complement(3414..4393)
                     /locus_tag="SDA06_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4398..5165)
                     /locus_tag="SDA06_001527"
     CDS             complement(4398..5165)
                     /locus_tag="SDA06_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4228804.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5655..6080
                     /gene="lacA"
                     /locus_tag="SDA06_001528"
     CDS             5655..6080
                     /gene="lacA"
                     /locus_tag="SDA06_001528"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG4228805.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6097..6612
                     /gene="lacB"
                     /locus_tag="SDA06_001529"
     CDS             6097..6612
                     /gene="lacB"
                     /locus_tag="SDA06_001529"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG4228806.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6623..7555
                     /gene="lacC"
                     /locus_tag="SDA06_001530"
     CDS             6623..7555
                     /gene="lacC"
                     /locus_tag="SDA06_001530"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG4228807.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7558..8538
                     /gene="lacD"
                     /locus_tag="SDA06_001531"
     CDS             7558..8538
                     /gene="lacD"
                     /locus_tag="SDA06_001531"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4228808.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8566..8883
                     /locus_tag="SDA06_001532"
     CDS             8566..8883
                     /locus_tag="SDA06_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4228809.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            8883..10589
                     /locus_tag="SDA06_001533"
     CDS             8883..10589
                     /locus_tag="SDA06_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4228810.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10731..12137
                     /gene="lacG"
                     /locus_tag="SDA06_001534"
     CDS             10731..12137
                     /gene="lacG"
                     /locus_tag="SDA06_001534"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG4228811.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12160..12432
                     /locus_tag="SDA06_001535"
     CDS             12160..12432
                     /locus_tag="SDA06_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG4228812.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12453..13352
                     /locus_tag="SDA06_001536"
     CDS             12453..13352
                     /locus_tag="SDA06_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG4228813.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4427 a         2298 c         2664 g         4304 t
ORIGIN      
        1 acattattag tttcgtcaga atcaaatatc aaaaattctt ctgttgttta tgtttatggt
       61 gacgaacgag aatatatcga atcttctgaa cataaatatg actttaaaat aggcaatata
      121 ctaacgtttg atgatcgagt tgcatatgac acgatgatag ataagaacga tgaaaaaagt
      181 ttgctcgtaa gttttttgtt tcccgaggta catgaaggtg ggcatctaga taccctgacg
      241 acatctgttg ttttcttggt atttaaaaat aagttgataa tttttacaaa acaaaaatta
      301 aaattcattc ccgaactctt atccaatatg gttttacgtg atgtcaatca tttcgtggag
      361 gaagtacttt taaagatcat gcaaaaagat ttctatatga tgcttaacga ccttcgtgga
      421 gtcaaggaaa aaattgatga tttagactca gaaatatcaa cgtccggttc actcaaacca
      481 acgtttggtg atttattgac tcttcaaaaa tacatgatag ctttgtcaac aacttataaa
      541 gctaaccata aagcgcttga tttcattaaa aaaaatttca caactattga cgatattgat
      601 tttagtacta aaaaaataat tgatgattta tacgatacaa gtgatacaat ggatagaatc
      661 attttaagtt acagccaata tttggacaaa ttggaaaata tgataaacaa tatgatttcg
      721 tatcagctaa acatgattat gaaaacccta accgagatat caattgtttt gactattcca
      781 gcaattattt ttggcttttg gggaatcaat gtccatgtcc cgtttgaaaa gtcttcttat
      841 ggtgttttag ccgtattgat tatctctgct atactaagtc taatttgctg gcttttgatg
      901 cgacgcaaaa catatttgta aagtatttgt gataccctaa tatgatttaa agcagaattt
      961 ctttgttgac tttttaaaag gctgattcct ttcaataatt taatagcttc agcggctgct
     1021 cagaagtcaa taaattggac tatttttatt cagttactta ggcaacattt gagatacgat
     1081 tgcgattata aaaattgata taccaaccaa tggctgcttg gaagactgac atattagcct
     1141 cgatatcatc gaataactaa atacagaaaa atacaataaa tattatcaaa ctagggacta
     1201 tactagaaaa catcagtaca ttccttaact cagggtctat aattagaaaa tcgccccttt
     1261 gaaaactgat taaaacagcc cccattatct agtgactaga taatgggggc tgtttttttg
     1321 ttaagtgata taataaacca taaaagaagt ttagaaatac ggtcctggtc atcaataaat
     1381 aaacaattta attaattatt tatttaatta tttaatttca gaaaggtata catgaatcat
     1441 ggtgcaacaa attgtcctac ccatcaaaga ctccagcgtc ttaaagatgg tacaagatac
     1501 acttctcgat agtttccgtg ctggtcgccg aaattacacc gtttttcaag ttggtaaggc
     1561 cacgctacta cgtgtgagtg atgtcatgac attaaaaaaa tcagatgtct ataatccaga
     1621 cggctctgtc aaaaatgcaa cctttattca tgataaaaag accggtaaag caaacacgtt
     1681 atatttaaag cctgttcagc aagacttgtt gcaatatcat gattggctgg cccaagaaaa
     1741 tatcaattct gagtggttat ttccctcgac agcccattat gatcttcata tcactgagaa
     1801 gcagtttcat aaggtgatgg cgcatgttgg tgatctctta ggaatcaact tggaagacaa
     1861 agagaaaaaa ttgcgagtca gaaaaccgtt ttgagatatg aacagcattt gccttcacgc
     1921 cttagcgatt ttaaataata gccttgaaag tgattatgga aaagccctct acgctcaatg
     1981 caaaattgac gtagaaacta tttttgatcg attgaaaggt gtatttggta tgtgaagaac
     2041 ccatgtcaga ggcaagaacg ttgcacctac tgacattgga atgatgctta tgagcatgaa
     2101 tctaaccaaa ttatcccttg aggctagaag aaaagcggaa gcttttcaac acaaagcagc
     2161 caaaaataaa aaacgcgacg aagcaatcac atttatgatt attcatcgcg ttttttatat
     2221 ttagagctag ttatttgcta gacccactat ttacagatga aacgtttatc acctttggtt
     2281 ttgggcattt agtggaatta gcagaaccag gtcattatga cgaaaagtgg caaaattgga
     2341 aacttgaatc tttgccgatt tttcctgatc gatacgattt tgaagttgca aaagataagg
     2401 gaaagcagtt taaaattgtt gcagaacttc tcaaaaaggc aaatacaatt attgttgcaa
     2461 cagatagcga cagagaaggt gaaaatatcg cctggtcgat tatccataat gcaaatgcct
     2521 tttcaaaaga taaaacattt aaaagactat ggatcaatag cttagaaaaa gatgtgatcc
     2581 gaagcggttt tcaaaatttg caaccaggaa tgaattacta tcccttttat caagaagcgc
     2641 aaacacgcca aattgccgat tggttgatcg gcatgaacgc aagccctttg tatacgttaa
     2701 atttacaaca gaagggcgta caaggtacat tttcactagg acgtgttcaa acgcccacct
     2761 tataccttat ttttcagcgc caggaagcca tagagaattt taaaaaagaa ccttttttcg
     2821 aggtggaagc taagtaaaac aagggtcatt taagggcgtt ctaagcccca cacagcgttt
     2881 taaaacccaa gaggagcttt ttagcttttg tttcttctaa acaagctaaa ataggcaatc
     2941 aagaggggat aattgctgat gttcaaacca aagagaagaa aacgaatagt ccgagttcgt
     3001 tttctttaag tagtttgcaa tcaaaagtca atcagcttta taaagcgaca gcgagccaaa
     3061 ctttaaaagc tatgcaagga ctgtatgaag caaaattatt gagttatcca agaacagata
     3121 caccatttat tacagagaac gaatttgctt atttaaaagc gaattttggc aaatatagcg
     3181 gttttttagg acttgatctt gaaatgttgt tatcattgat aatgatacgt tcaaacaaca
     3241 gcacccaaat tttgacgaat tagtgaaact ctatgaaaaa gacgtagtaa aacatgcaac
     3301 atcctattct aatcagctgg taaagcttaa ttaggttata taagcttgaa aataaagggt
     3361 ttgtccaata tggacaagcc tttttcttat gttttgccac agatttgcca cacttattgt
     3421 gtaagagtat caaggatagg tataatttga tactttgttt ttttagtgat gtgcaaataa
     3481 atttctgtta cacgactacc tctagaatgc cctacacggt cttgaattgc ttctaggggt
     3541 attccttact ctgctaagaa actaatatgc gtatgacgga atatatgggt cgtaatatgt
     3601 ttattaaaac taagtttatt atcaatatca attcctgctc gttttggatt accttttcga
     3661 tttaattttc cattttcagg tgagttgagg aaattgttaa ggacactata atgaagaggt
     3721 gttccattat agtcgggttt atgacgactt ttggaagtaa aaatccatcc attatcttta
     3781 taatcaggat tgaatctcat atgttggtga tttaactcaa tttcctcttt aagaatttct
     3841 atagcccttt tagataattg gacatttcgg taactttgta agttttttac attaccttca
     3901 tagctatttt ctgcagctcc tccgtgagtg gactgactaa tgatgatatt ttgcttttca
     3961 aaatattgat aggcaccaat attttggagt ttcgactctg taagtcaaca gggccattag
     4021 ttagaatacc tattgggatg cggtttttat gtagagccag cagtagttga tgtatctcag
     4081 gaatcatcgt aatctgatcg agttcatatt catagtcagc ttggaagtct agagcttctg
     4141 tcctagaaat actatcgatg ccaaattctt ccatagtctt ctgacagcga aaaattctaa
     4201 gctgctcaat actgatgagt tttttcgtgt atttaggaaa agcaacatca gaccaatatc
     4261 tgaaacgttt gtaaatgtta tctatttgtt ggatatcaat cgttggaaaa catttctcaa
     4321 cgctacgttt aaaggggagt tgatggtcat aaagagtgtc atcaaggtca aaaacaatat
     4381 ttttataagt cataattcta gtccgctttt aaaatagtag tgtactggct aaattcctct
     4441 aagtgttgag ggctaatctg gttatctgta atgatggtat cgagttgatc tagattatag
     4501 aagttaaaga aatcgtatcg atcgaattta gtactgtcta ctaataagaa tttttctaca
     4561 gcattgttta gggcaagttg ttgaatcaca ccttccttgt cactatatgt agcaatagaa
     4621 ttatgggtaa cagcatttgc actaacaaaa gctttggcaa atctaattgc ttttaaattt
     4681 gtcgaagcca ttgaacctac aaaagctcca gttatttctc tatattcacc gccaagaagc
     4741 aataaatcaa tggtttcgct atcatttaga atcaagaaca cagggagact atttgtaatg
     4801 acacgaattt tataaccttt acgacctttc aattctaatg ccagttgttc aagtgtagtt
     4861 ccaggtccaa taaaaatagt atcgccatct ttgattaaag agcaggcttt tttagcaatg
     4921 tcaatttttt cttttgtatt tagacttttc ttctcgatat gtgtcttttc aagtggcttt
     4981 ttagaggaaa gcaattgtgc accaccatga gtacgtgtta aaagtccatc agcttctaga
     5041 gcatcaaggt ctctacgggc tgtcatatcg gaaatatcta gttcatctat tatttctttt
     5101 atggttatgg tcccatcaat ctttaacata tctaaaattt tttctaatcg tcgttttttg
     5161 ttcatatgaa gacccttctt tcataaagta atttttttcc aaagataatt ctcttttaat
     5221 tgtatcataa aagacaatat tttcaaggta aaacaaacaa tttcaaacaa aaacaaatgt
     5281 tagatgatga aataagaaca gaggattgac gtatattagc ttaggtcaga ttttgtataa
     5341 gacgaaaata aagtaggacc tcttaatcag taagttatag aaagtaaaag acttttgtaa
     5401 tacttgaata gatatttcac gtccattttg tgatggatta aatgaacaaa aatgaacaat
     5461 aatttaacgg tgttatctat tttttaaaaa aacaaataaa aaaaacaaaa aataaacaaa
     5521 aaaagtgttg ctttttgttt gaatgcttga tattatataa acaaagaaat gatgaaaacg
     5581 ttttttcgaa cattttgcaa aatgttttct acttctacgt agcatttctt tctaaaattt
     5641 aggaggtggt caaaatggct attgttgttg gtgcagatct caaaggtacg agattaaaag
     5701 atgttgtgaa aaattttctt gtagaagaag gttttgaggt aattgatgtg actaaggacg
     5761 gacaagattt tgttgatgtt accttggcag ttgcctctga agtaaataaa gatgagcaaa
     5821 acttaggtat tgtaattgat gcttatggag ctggtccatt catggtagca actaaaatta
     5881 aaggtatggt tgcagcagaa gtttcagatg aacgctcagc ttatatgaca cgtggacata
     5941 acaattcacg aatgattacg gttggcgctg aaatcgtcgg tgatgagctt gctaaaaaca
     6001 tcgctaaagg tttcgtcaat ggtaaatatg atggcggacg tcatcaaatc cgagtagata
     6061 tgcttaacaa aatgtgttaa gaaaaggagt taaggaatga gaatcgcaat tggatgtgac
     6121 cacattgtca cagatgaaaa aatggcagta tcagaattct tgaaatcaaa aggacatgag
     6181 gtccttgatt tcgggacata tgaccacgca cgtactcact accctatcta tggtaaaaaa
     6241 gtcggtgaag cagtagtaag tggtcaagca gacttgggag tatgtatctg tggtacaggt
     6301 gtcggcatta acaatgcagt caataaagtt ccaggtgttc gctcagcgtt ggttcgtgat
     6361 atgacatcag ctatatatgc aaaagaagag ttaaatgcga atgttatcgg tttcggtgga
     6421 aagattactg gtggtcttct tatgaatgac atcattgaag ctttcattga agctgagtac
     6481 aaaccaacag aagaaaataa aaaattgatt gcaaaaatcg aacacgttga aacacataat
     6541 gcacatcaag cagatgaaga tttctttaca gaattccttg aaagatggga ccgtggtgac
     6601 taccacgatt aaggtataac aaatgattct gacagtcaca ctaaatcctt cagtagacat
     6661 ctcttatcct ctagaaacat tgaaaattga tacagtgaat agggtgaaag atgttagtaa
     6721 gacagctggt ggaaaaggct taaatgttac tagagtcttg tatgaatctg gtgataaagt
     6781 tactgctaca ggtttcttag gtggtaaaat tggtgaattt attgaaagcg aattagaaca
     6841 atctcctgtt agtccagctt tttataaaat ctcaggaaac actagaaact gtattgctat
     6901 cttacacgag ggtaaccaaa cagaaatttt ggaacaagga ccaacaattt ctcatgaaga
     6961 agcagaaggc tttcttgacc actatagcaa tcttattaaa caatcagaag ttgtgacaat
     7021 ttcaggaagt ttaccttcag gacttcccaa cgattattat gaaaaactta tccaacttgc
     7081 ttcggatgaa ggagtagcag tcgttttgga ttgttcaggt gcacctcttg aaacagttct
     7141 aaaatcgtcg gctaaaccta ctgccatcaa acctaataat gaggaacttt cacagttgct
     7201 tggaaaagaa gtaacaaaag atattgaaga actcaaagac gttcttaaag agtcactttt
     7261 tagtggtatc gaatggatag tggtctcatt aggtcgaaac ggtgcctttg caaaacatgg
     7321 tgatgttttc tataaggtag atattcctga tatcccagtt gttaatccag tcggatcagg
     7381 ggactcaacg gtagctggta ttgcatcagc tttaaatagt aaaaaaagtg acgctgactt
     7441 attaaaacat gcgatgacat tgggtatgtt aaatgctcaa gaaacaatga cagggcatgt
     7501 gaatatgact aattacgaaa cactaaactc acaaattgga gtaaaagagg tataaaaatg
     7561 gtacttacag aacagaaacg caaatctttg gaaaaaattt cagataaaaa cggtgttatc
     7621 tcagctttgg catttgacca acgtggtgct ttgaaacgtt tgatggcaca gtatcaagat
     7681 acagaaccaa ctgtagctca aatggaagaa ctaaaggttt tggttgctga tgaacttaca
     7741 aaatacgctt cttcaatgtt gcttgaccct gaatatggac ttccagcaac aaaagcattg
     7801 gataaagaag caggtcttct tcttgcctat gaaaaaacag gttatgatac atcaagtaca
     7861 aaacgtttgc ctgactgctt ggatgtttgg tctgcaaaac gtatcaaaga gcaaggcgct
     7921 gatgccgtta agttcttgct ttactatgat gtagacagct cagatgaact taaccaacaa
     7981 aaacaagctt atattgaacg tgttggttct gaatgtgtcg ctgaagatat tcctttcttc
     8041 ttggaaatcc ttgcttatga tgaagaaatc tcagatgcag gttctgttga atatgcaaaa
     8101 gtgaaacctc gcaaagtcat tgaagcgatg aaagttttct ctgatccacg ctttaacatt
     8161 gatgttctta aagtagaagt accagttaac gttaaatatg tcgaaggttt tgctgatggt
     8221 gaagtagttt acagtaaagc tgaagctgct gacttcttta aagcacaaga agaagcgact
     8281 aaccttccat acatctacct aagtgcaggt gtatctgcta aattgttcca agaaacattg
     8341 caatttgctc acgactcagg tgccaagttt aatggtgtgc tttgtggacg tgctacttgg
     8401 gcaggatctg tagaacctta catcaaagaa ggtgaaaaag cagcacgtga atggttgcgt
     8461 actactggat tcgaaaatat tgatgaactt aacaaagttc ttgttaaaac agctagtcca
     8521 tggactgata aagtatagta aaaacataaa acggaggata ttgttatgaa cagagaagag
     8581 atgactctct tagggtttga aattgttgct tatgctggag atgctcgctc taaactttta
     8641 gaagcgctta aagcggctga aaatggtgat ttcgctaagg cagatagtct tgtagtagaa
     8701 gcaggaagct gtattgcaga ggctcacaat tctcagacag ctatgttggc tcgagaagct
     8761 tctggggagg aacttccata cagcgttacc atgatgcatg gtcaggacca cttaatgact
     8821 actgtcttat taaaagatgt gattcatcac ctcatcgaac tttataaaag aggagcaaaa
     8881 taatgcataa actcattgaa cttattgaga aagggaaacc gttctttgag aaaatctctc
     8941 gaaacatcta tcttcgtgct attcgtgatg gatttatcgc tggtatgcca gtcatccttt
     9001 tctcatctat ctttatcctt attgcctatg tcccaaatgc ttggggattc cactggtcaa
     9061 aagacattga gaccttcttg atgactcctt atagctattc gatgggtatc ctagcattct
     9121 ttgttggtgg tactaccgct aaagctttga cggactcaat gaaccgtgac ctacctgcga
     9181 ccaatcagat taacttctta tctacgatgc tagcgtctat ggtagggttc cttttgatgg
     9241 cagctgagcc tgcaaaagaa ggaggcttct tgacagcctt cacgggaaca aaaggtcttt
     9301 tgacagcatt tattgcagcc tttgtgacag ttaatgttta caaggtctgt gtgaaaaata
     9361 atgttactat tcgtatgcct gaagaggttc caccaaatat ctctcaagta tttaaagact
     9421 tgattccatt cactgtagcg gttgttctcc tttatggatt tgaactcatt gttaagggaa
     9481 ctcttggtgt aaccgttgca gaatcaatcg gtacccttct tgctcctctc ttctcagctg
     9541 cagatggtta ccttggaatt acacttatct ttggtgctta tgccttcttc tggtttgttg
     9601 gtatccacgg tccttcaatt gtcgaaccag caatcgctgc tatcacttat gccaatatcg
     9661 atgccaactt gcatcttatc caagctggac aacatgcaga caaagttatc acttctggta
     9721 ctcaaatgtt tattgttacc atgggtggaa caggagctac attgattgtt ccattcttgt
     9781 tcatgtggat ttgtaaatca gaacgtaacc gtgccatcgg acgtgcctca gttgttccaa
     9841 cattctttgg ggttaatgag ccaatcttat ttggtgcacc aatcgtattg aatccgattt
     9901 tctttgtacc gttcattttc gctccgattg tcaacgtttg gatctttaaa ttctttgttg
     9961 acacattgaa catgaactca ttctctgcca accttccatg ggttactcct ggtccgttgg
    10021 gaattgtact cggtactaac ttccaagttc tatcatttat cttagccgga ctcttggtag
    10081 ttgttgatac tatcatttat tacccatttg ttaaggtata cgatgaacaa attcttgaag
    10141 aagaacgttc tggtaaaaca aacgatgctc ttaaagaaaa agtagctgca aacttcaata
    10201 ctgctaaagc agatgccgtt cttggaaaag caggcgttgc taaagaagat gtagcagcaa
    10261 acaataatat cacaaaagaa actaatgtcc ttgttctttg tgcaggtgga ggtacaagtg
    10321 gcttgcttgc caatgccttg aacaaagcag cagcagaata caatgttcca gtcaaagcgg
    10381 cagcgggtag ttacggtgct caccgtgaaa tgttgccaga gtttgacttg gttatcttgg
    10441 ctccacaggt cgcttcaaac ttcgatgata tgaaggctga aacggataaa ttgggcatta
    10501 aacttgcaaa aactgaagga gctcaatata tcaaattgac acgtgatgga caaggtgctc
    10561 ttgcatttgt tcaacaacag tttgattaat aaatttgtaa aagttatttg agacgagatt
    10621 attctttcaa ttccttataa tattgttaac catatcattg catttcattt ccttggtttc
    10681 cggaactcag aatatcgtct caaatgcttt ttttgaaagg atttatagtt atgactaaaa
    10741 cacttcctaa agattttatt tttggtggtg ctacagctgc ttatcaagca gaaggtgcga
    10801 ctcacacgga tggtaaaggc ccagttgcat gggataagta tcttgaagac aattattggt
    10861 acacagcaga gcctgcaagt gatttttacc ataaatatcc tgttgattta gaattagctg
    10921 aaaaatatgg cgttaatggt attcgtattt caattgcctg gtctcgtatt ttcccaactg
    10981 gctatggcga agtaaatgaa aaaggtgttg aattctacca taatcttttt gccgagtgcc
    11041 acaaacgtca tgttgagcca ttcgtgactt tgcaccactt tgacacacca gaagttcttc
    11101 actcagatgg agatttctta aaccgcgaaa atatagaaca ctttgtagat tatgccgctt
    11161 tctgttttga agagttccca gaagtaaact actggacaac tttcaatgaa attggcccaa
    11221 ttggagatgg tcaatacttg gttggtaaat tccctccagg cattcaatat gatcttgcca
    11281 aagtcttcca atcacaccac aatatgatgg tttctcatgc acgtgctgtg aaattgtata
    11341 aagataaagg ttataaaggc gaaattggtg tagtacatgc gttgccaacc aaataccctt
    11401 atgatccaga aaatccagca gatgttcgtg ccgctgagtt agaagatatc atccataaca
    11461 aatttatctt ggatgcaacc taccttggac actattcaga taaaacgatg gaaggggtca
    11521 accacatcct agctgaaaat ggtggagaac ttgatcttcg tgatgaagac ttccaagcac
    11581 ttgaagcagc taaagatttg aacgatttcc ttggtatcaa ctactacatg agtgattgga
    11641 tgcaagcctt tgatggtgag actgaaatca ttcacaatgg taagggtgaa aaaggaagtt
    11701 ctaagtacca gattaaaggt gttggtcgcc gaatcgcacc agattatgtg cctagaacgg
    11761 attgggactg gattatttat cctgaaggct tgtatgacca aatcatgcga gtgaaaaatg
    11821 attatccaaa ctacaagaag atttacatca ctgaaaacgg tcttggatac aaagatgagt
    11881 ttgtagataa taccgtttat gatgatggtc gtattgatta cgtgaagaaa cacttggaag
    11941 ttttatcaga cgcaattgcg gatggtgcaa atgttaaagg ttacttcatc tggtcactta
    12001 tggatgtctt ctcatggtca aacggatacg aaaaacgtta tggactattc tatgtagact
    12061 ttgacacgca agaacgctat cctaagaaat cagctcattg gtataagaaa gtagcagaaa
    12121 ctcaagtgat agagtaatat tacttgaaaa tgtaaaaaat tgttatccga tactttagaa
    12181 aaagtacaac acttatttag accaggcttt gaaaaggtat atattgttag tttcgaagat
    12241 tgtcctatga ttcctgagct tgaagctacc ccgttgctaa aatgtggtaa atggtatgtt
    12301 tcaactggaa aagagtggat ttgccattct gatttagagt tatcagcatt tgaatgggaa
    12361 tttttacaat cactcgatgt agaaatacgt gagactattc acttcgaggt aaattatttg
    12421 cctttccaat aataaaaaga aaggaattag ttgtgactat cgaattaaaa aacgagtatc
    12481 ttactgttca gtttaaaaca cttggtggtc aactgacttc gattaaagac aaggatggga
    12541 tagagtatct ctggcaagct gatccaaatt attggaatgg acaggcgcct attctatttc
    12601 ctatctgtgg tagtttacga aatgattggg ctatttatag acctcaagaa agaccctttt
    12661 ttacaggtct tattcgtaga catggttttg ttcgaaaaga agaatttatt ctggaagaag
    12721 tcaatgataa tagcgtgacc tttagtatta aaccaaatgc tgagatgctt gataattacc
    12781 tatatcagtt tgaactgaga gttgtttata ccttgaatgg taaatcaatt agaaccgaat
    12841 ttcaggtaac gaacttggaa atggaaaaaa caatgcctta ctttattgga gcccatccag
    12901 cattcaatag tcctttagta gaaggtgaaa actatgagga ttattatatt gaatttagtg
    12961 aggttgagtc ttgctcgata ccaaagagtt ttcctgagac aggattatta gatttgcaag
    13021 atcggacacc ttttttggaa aatcaaaaaa ttctcgattt ggactactca cttttttccc
    13081 atgatgccat tacacttgac cagctcaagt ctcgtagcgt aacgcttcgg tcaaggaagt
    13141 ctggtaaagg tcttcgagta gattttgatg attttccgaa cttgattctg tggtctacaa
    13201 ctaataaaag tccattcatt gctttggaac cttggagtgg tctctccact tcacttgaag
    13261 aaggtaattt cttagaagat aaaagacagg taacgaaaat ttcacctcaa gaaacttcta
    13321 gaaaaatcta tgatattact atccttaatt aaaatttgtt attatttgag tcttcaataa
    13381 gatttgaata ctcttacctt gtgatgatag attggtcttc tccgtgccag tcttacatcg
    13441 caaggttttt aagttttttg tgggtctatg tcaactgtag tgggtgacga aaagctaata
    13501 tctaaagaca gtctggtagt aaaaaataag attatttact acttcttgat ttaaaataat
    13561 agtggagcca ttaatttgtg gcaaatttaa tttaatattt tgcctcaaat tagccacact
    13621 ttttatagaa ctaagaggaa aaatgaaatt ttcttcctat taatatagtt gtttgaaatc
    13681 ttaaatatag cgt
//
LOCUS       DARDHD010000069        13196 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000069 DARDHD010000000
VERSION     DARDHD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.069"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            85..1824
                     /gene="yfcC"
                     /locus_tag="SDA06_001537"
     CDS             85..1824
                     /gene="yfcC"
                     /locus_tag="SDA06_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4228814.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1981..3165
                     /gene="iadA"
                     /locus_tag="SDA06_001538"
     CDS             1981..3165
                     /gene="iadA"
                     /locus_tag="SDA06_001538"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG4228815.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3276..3479
                     /locus_tag="SDA06_001539"
     CDS             3276..3479
                     /locus_tag="SDA06_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4228816.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3580..4242)
                     /locus_tag="SDA06_001540"
     CDS             complement(3580..4242)
                     /locus_tag="SDA06_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228817.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4255..5757)
                     /locus_tag="SDA06_001541"
     CDS             complement(4255..5757)
                     /locus_tag="SDA06_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228818.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6305..7984
                     /locus_tag="SDA06_001542"
     CDS             6305..7984
                     /locus_tag="SDA06_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4228819.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8111..8590
                     /locus_tag="SDA06_001543"
     CDS             8111..8590
                     /locus_tag="SDA06_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG4228820.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8809..9489
                     /locus_tag="SDA06_001544"
     CDS             8809..9489
                     /locus_tag="SDA06_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG4228821.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9497..10828
                     /locus_tag="SDA06_001545"
     CDS             9497..10828
                     /locus_tag="SDA06_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4228822.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10843..11805
                     /locus_tag="SDA06_001546"
     CDS             10843..11805
                     /locus_tag="SDA06_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG4228823.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11916..12254
                     /locus_tag="SDA06_001547"
     CDS             11916..12254
                     /locus_tag="SDA06_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG4228824.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12333..13040
                     /locus_tag="SDA06_001548"
     CDS             12333..13040
                     /locus_tag="SDA06_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG4228825.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4189 a         2333 c         2898 g         3776 t
ORIGIN      
        1 aaatgtcatt tttactgtat aattcatttg attatccatt ttttattcat caaaattact
       61 gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc
      121 gacttcacta aattatcgac accccatacg tatgtcatta tttttggtgt agtcatcttt
      181 gcatggatac tgacttttgt cgttccagca ggaaagttca gcacgcaaga tattgaatac
      241 aaagatgcaa atggcgaaac aagtactcgt actgttttac gtcaagattc cttccgttat
      301 gcttacgagc tggataagtc gtatgtgttt gatcaattag aagaactgca agatcatcca
      361 gcagaacgtg aaaaactgga tgttccggaa aaaggattgg aaaaagtcat tgctgacgga
      421 gaaaaaaatc ttacgcagga aaaactagat gaaatctcct tgacagatga tgtgctttac
      481 gatcagtacg gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata
      541 tggggaacag atgattttgg cggctttggt ttcttaaatt ttgtttttga aggactagta
      601 tctggagata agtatggatc tgctgtaggg atcgctgcat tgatccttgt tgttggaggt
      661 gcttttggta ttattatgcg taccggagcg atcgacgcag gtatctatgc gtttatcagt
      721 aaaactaagg gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt
      781 ggcgctacat tcgggatggc agaagaggta attccattct caatggtgat ggttccattt
      841 gtcattgcgt taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa
      901 gtcggaaatg cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct
      961 ggaatccccg tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca
     1021 ttagccgctg gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg
     1081 ttgacttaca aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat
     1141 aaaaaattca tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg
     1201 atcgtttggg gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt
     1261 gtgatgggat tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc
     1321 aatgacatcg cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta
     1381 gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca
     1441 gctttgaata cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt
     1501 ggtgcttggg cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca
     1561 ggacaagcag ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc
     1621 agacaagtag cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc
     1681 gtatcagcca gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca
     1741 agattccaaa tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt
     1801 gccattgcaa taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca
     1861 gtagataagg actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat
     1921 cgtttggatt attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt
     1981 atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta
     2041 ctgattgccg gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga
     2101 gtggaagtag aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt
     2161 catttccata ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc
     2221 acattgagcc aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac
     2281 ggagaaggta gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga
     2341 atctccactt atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct
     2401 atcatcaaag actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc
     2461 gatcaccgtt cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt
     2521 gtaggaggca tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa
     2581 agaaagctag agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt
     2641 ttgcctactc atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa
     2701 cgtggtggca cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat
     2761 ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat
     2821 aatttcacga tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat
     2881 ttcctagggt taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg
     2941 caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt
     3001 atattgaaat tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc
     3061 ttggataaag aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa
     3121 gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac agctctaact
     3181 atctttttta gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt taccctgaag
     3241 gtagatcaat aaacaaacta agaggaggaa gcacaatggc agatttaaaa ggacgttcta
     3301 aagatatgaa agataaagta gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg
     3361 acaagagcaa agaaacagaa gggaaagcac aatcactagg tgctgacatc aaagaaaaag
     3421 ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa
     3481 aaccacttat tactaattga atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag
     3541 atatggttcg gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa
     3601 ttttttattt tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt
     3661 cacgatgata atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc
     3721 ttgtgaagtt gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc
     3781 acagcagata gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac
     3841 ctgttttttt gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg
     3901 tcgataattc tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc
     3961 cgataaataa ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata
     4021 acttcttaac ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg
     4081 tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa
     4141 aatagcgtac atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg
     4201 cggatcttga taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt
     4261 taataggatt tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa
     4321 cagactgatt ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat
     4381 ttccaattcg ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc
     4441 atcgcgttga ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg
     4501 ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc
     4561 gacgacgttt ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc
     4621 ggtaaacaat tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa
     4681 ctcatgccga cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc
     4741 tacagccact agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa
     4801 cattgactga tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt
     4861 ctcagcttct ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga
     4921 atctatcgtg ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta
     4981 aagtgtattc gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct
     5041 ggattcataa attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt
     5101 gattgtgaaa gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac
     5161 tacattgttt gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt
     5221 tccatcagaa aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc
     5281 ttgagtcatt tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt
     5341 ttctgaactg tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat
     5401 agctgaggcg gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc
     5461 cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt
     5521 ggtagaaccg cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact
     5581 taaagtcact gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct
     5641 tcgaatcgtg aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa
     5701 caatgaacgt ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt
     5761 ttgcatccct cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa
     5821 tcttatgctg tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt
     5881 tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga
     5941 aagaggaata gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt
     6001 ctaacaattg ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg
     6061 aatacttata taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag
     6121 tatatccaaa ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct
     6181 gctgattctc cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat
     6241 atatttagat aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa
     6301 gtttgtgagt accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat
     6361 gtatgtcgca gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga
     6421 aaatgagttg ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat
     6481 tgatcgtgta gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc
     6541 ttacttgttg tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc
     6601 aaaattgaat gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga
     6661 tgagcataca gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac
     6721 gataccagat tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg
     6781 agattttaaa tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc
     6841 ggaaatcggc aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc
     6901 tcagcctgta gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg
     6961 ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt
     7021 ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat
     7081 tattcgcaca gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac
     7141 gttgaaagac atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat
     7201 gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat
     7261 tgaagaagga gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt
     7321 ggcgaaaacg gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa
     7381 tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa
     7441 accaacatat tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt
     7501 ggctcatgca gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct
     7561 ggaatataag aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat
     7621 tgatggatta ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc
     7681 agaagacggg attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc
     7741 aaaacctcaa attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa
     7801 agaaagcagc aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga
     7861 gtggagcaaa ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac
     7921 aaaacgccgc ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa
     7981 ataaacaaaa agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg
     8041 aaaaacaaat aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg
     8101 gagtgaatca atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag
     8161 ctatcaacca cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa
     8221 aagagggatt gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg
     8281 ggtgatcatc gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt
     8341 gacgaaagat cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata
     8401 tccccacgga gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt
     8461 agcagacagt tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag
     8521 aagccttcgg ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt
     8581 aattagttaa gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat
     8641 atctgcttgg tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta
     8701 ctatcaatcc aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg
     8761 aaatcgtttt taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg
     8821 tatcatggac attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac
     8881 ccgtttatta caggaaatcc attgacagac ttaactgcag aagaagtaaa gacagattgg
     8941 ttgctgatta ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa
     9001 aatcaagcca caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta
     9061 gattcattcg gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc
     9121 gtacatgcac agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag
     9181 cctagcggaa ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct
     9241 tatttcagtg atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg
     9301 attggcgata actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt
     9361 gccaagaaag tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa
     9421 tcttttgtag agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg
     9481 gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt
     9541 acctgttggc aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg
     9601 aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg
     9661 agtagggacc atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg
     9721 cgaggtcgtc aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt
     9781 cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc
     9841 aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg
     9901 cgccgcagta ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga
     9961 tgatcttgaa atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat
    10021 caaccgagcg ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta
    10081 tatgccagct gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg
    10141 tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat
    10201 ggggattgtt acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt
    10261 gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca
    10321 aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg
    10381 cttgattacc cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat
    10441 tgcagatacg atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga
    10501 aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg
    10561 cgttggaacc atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac
    10621 aatggtgatg aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg
    10681 gctgatccaa ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg
    10741 ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt
    10801 tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa
    10861 atagtagata caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat
    10921 cctgatgcat taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa
    10981 aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa
    11041 gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca
    11101 cccagaatca gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat
    11161 cccaatgatg acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt
    11221 gagatgattg cagaatttgc tttgatgttt cctgaatctt taaagatgaa cgttaaggct
    11281 gcacggttac tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg
    11341 acgtcccgta cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct
    11401 ttgaacagag agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat
    11461 caaaatatcc aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt
    11521 gataaatatg gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt
    11581 gacgaagtgc tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt
    11641 ttacgttcta aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat
    11701 ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag
    11761 atccaagata tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa
    11821 cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca
    11881 atagtgctca ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat
    11941 gtggctcaga gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg
    12001 aatggaacaa agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa
    12061 atggaaaccg tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag
    12121 gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg
    12181 atcataatat cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg
    12241 tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata
    12301 aaataaagta aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt
    12361 tagatccgac atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt
    12421 acgtcaatag tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta
    12481 cacaagcagc acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa
    12541 ttgccggaga tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag
    12601 caactgcaca gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg
    12661 cggtacaaga tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag
    12721 ctaactttgg ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga
    12781 actctacgtt aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg
    12841 tgacattacc agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg
    12901 gactactgac ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga
    12961 cttatgttgc agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg
    13021 gcggcaaccg aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa
    13081 taccgagagt tagctgattg aataccattt atttggaata agggctcttt gtcaataagg
    13141 actgatgggt tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcag
//
LOCUS       DARDHD010000070        13122 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000070 DARDHD010000000
VERSION     DARDHD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.070"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            254..460
                     /locus_tag="SDA06_001549"
     CDS             254..460
                     /locus_tag="SDA06_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG4228826.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(662..1663)
                     /locus_tag="SDA06_001550"
     CDS             complement(662..1663)
                     /locus_tag="SDA06_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4228827.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1668..3581)
                     /locus_tag="SDA06_001551"
     CDS             complement(1668..3581)
                     /locus_tag="SDA06_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4228828.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3749..4255)
                     /locus_tag="SDA06_001552"
     CDS             complement(3749..4255)
                     /locus_tag="SDA06_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4228829.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4415..4855
                     /locus_tag="SDA06_001553"
     CDS             4415..4855
                     /locus_tag="SDA06_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4228830.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5014..6354
                     /locus_tag="SDA06_001554"
     CDS             5014..6354
                     /locus_tag="SDA06_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4228831.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6423..7580)
                     /locus_tag="SDA06_001555"
     CDS             complement(6423..7580)
                     /locus_tag="SDA06_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4228832.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7583..7939)
                     /locus_tag="SDA06_001556"
     CDS             complement(7583..7939)
                     /locus_tag="SDA06_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228833.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8237..9211)
                     /gene="sgrA"
                     /locus_tag="SDA06_001557"
     CDS             complement(8237..9211)
                     /gene="sgrA"
                     /locus_tag="SDA06_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG4228834.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9407..10246)
                     /locus_tag="SDA06_001558"
     CDS             complement(9407..10246)
                     /locus_tag="SDA06_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4228835.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10431..11318
                     /locus_tag="SDA06_001559"
     CDS             10431..11318
                     /locus_tag="SDA06_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4228836.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11902..12597)
                     /locus_tag="SDA06_001560"
     CDS             complement(11902..12597)
                     /locus_tag="SDA06_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG4228837.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12581..12979)
                     /locus_tag="SDA06_001561"
     CDS             complement(12581..12979)
                     /locus_tag="SDA06_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4228838.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3927 a         2651 c         2034 g         4510 t
ORIGIN      
        1 cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat attcccatat
       61 tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat tgctgaatca
      121 ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat tttattcaca
      181 tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa ttataaaaaa
      241 ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta gtgcattgat
      301 tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa tattagtgga
      361 accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg caggcttcgg
      421 atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat ttacgtaaaa
      481 gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg ccccttgcca
      541 tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct tgtttattga
      601 aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc atcataagac
      661 tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt ttattgctga
      721 aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct acttttgtga
      781 ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc gtaaacgtat
      841 ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg
      901 ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa gcccatccac
      961 atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca gaaggatcca
     1021 acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat
     1081 gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca ctaatttgtg
     1141 cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct tttatctctt
     1201 ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt tttgcttccg
     1261 gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat agatattcca
     1321 aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc atccagaaaa
     1381 gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta ttatttttgt
     1441 actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt tgctggaaat
     1501 catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt ctaacaagca
     1561 agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa ataactacga
     1621 tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta ccctctcttt
     1681 cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga ataaatgaga
     1741 agcgaatgtt tcttttctcc agttttcggc aacattcttt tttttgcatc tgtctttttt
     1801 tgtactttca ctgtcatctg aatcggttgc gtatcatcac ttgattgatc ctctcgaggt
     1861 tcattctttc ccttattgtc tgcattctct gtcggatctt gcttttttgg tggagctact
     1921 gggatttttt tgtaatagaa aataatagtt ggtatcgtct cattaaaaat cccttcagtc
     1981 ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct tttgctctgt catataattt
     2041 tctccataag ctccgtaaag ctggatttcg tcctggtctt tgagtagact tttattgttt
     2101 tcatccacat actttacgtt tatctgatca tggaacacta ctttcaaaaa gtccgttaca
     2161 tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc catgccctgc tccttctact
     2221 aataaaagat ctttataccc tcctgataac tcatataatt tttgcgcgtt agacacaggt
     2281 actacatcat ctgctgttcc atggataatc aatttaggca atgaatctga cattccaatc
     2341 gtaggtagag cagcttttgt atccaaatgc atctcctgat caaaatagta ttgtttcaaa
     2401 aggcccataa ataaatcttc atgctcttga tccccaatga tgtctaactg attttttatg
     2461 gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt
     2521 gatgcaaatc cgcaattttc aataattcct ttgacattga caggaatatc tttcgctaat
     2581 acagacatca tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc
     2641 cgctctcgaa cttcttgatt tatccagtaa aggacgtcat ctgactcata ttgtccatag
     2701 gtcacataat cgcctccgct attaccagtc gctctggaat caacaaataa taaattatac
     2761 ccggccttat aaaaatcatt gtattcttct gtaacgatcc cgttattcca attccctcga
     2821 aatccgccat ggatcatgac tgttttatct gaaccttgat cgacataata tgcatataca
     2881 tccatcattt tgccatcttg tttgtttagt acagtttgat gaagttgttt tgtatttgga
     2941 tagaagcctg tgttttctgt aggcgttgtc ccatcatctg tacctccaag catcgacatc
     3001 aacacactgt tttttgcaaa agcgatatcg tacaatagat aagtagcaat cagatgtttg
     3061 ataatctctt tatctacacc ataatcatta acagcaagct cagtgactaa taactctatt
     3121 aattttgact tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg tttaccgaag
     3181 taattcaaat attctttacc attgaaattt tcacctttac cgatatcatc attttctgtc
     3241 ccattctctt tgtcacttac tttagcagaa gaggattcgt cgtttgacat ttttgtagct
     3301 gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc tgtgctagta
     3361 acagatgtct caaccgttgt ttcagtattt gtttctgttt gtgaagtact cgatttcgtt
     3421 tcatttccag catcagtagt ttctgtggtt tctacctccc gagattccga ttcggaagaa
     3481 atgctctctc catatactgt ccattgcggg acgcttgtac ttacagcaaa taagatgacc
     3541 aacgctccct ttaatacttg ttttgttctc aagctattca ctccttttta tgtttctagg
     3601 agaattatat gtaaacgcaa acatttttta tacaaaaaat aaatggttta tcccataaaa
     3661 atagaacagg attattttct cattttacgt aataaagcag aatggataaa ccagtatcca
     3721 ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga aatcgtttat
     3781 cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga ttatcataag
     3841 ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca aacttcacag
     3901 tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga cagtccaatt
     3961 cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa
     4021 aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt gtttgtggca
     4081 ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt
     4141 tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc gaagaatcaa
     4201 aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt gacatacttg
     4261 tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt ccattatacg
     4321 tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt cttcatgtta
     4381 ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt atacaggttc
     4441 tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata taccgtttat
     4501 cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact tactaaaatt
     4561 gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata aaaatcttgt
     4621 tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta catccccttc
     4681 tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg gatttagcga
     4741 agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca acgaactctt
     4801 gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt cttaattttg
     4861 gcataaacaa tctgcttttc tggatctata atatctagtg ttggtgacgc cttctgttgc
     4921 caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct ttagaataaa
     4981 gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag
     5041 ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg caaagagcgc
     5101 attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc
     5161 ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc
     5221 atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa acagcgcttc
     5281 aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga tcgtcgctgc
     5341 tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc tatttctatg
     5401 tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc
     5461 cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa
     5521 ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc
     5581 catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg
     5641 cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt ctatacaccc
     5701 tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat
     5761 attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt
     5821 aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact tcaaaagaat
     5881 gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca
     5941 gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg aggctacgaa
     6001 gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc
     6061 ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac gaccttcaag
     6121 aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg ccctatagaa
     6181 tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag aaactttcaa
     6241 aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac taagaacact
     6301 acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt ctagccgtgt
     6361 gagtatctcg attattctat tgaatttctt caccaacact cattgacgaa gagccgcttt
     6421 tcttaattaa cgaaatccaa tttttccaat atggatctat ttttctcatt cctgattttt
     6481 atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt ccagtaaaat
     6541 atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct aatgacaata
     6601 tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat agataatatc
     6661 cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata gaatatgaca
     6721 ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa aatcaaccgt
     6781 ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc actaaaaaac
     6841 tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc ttctgctgat
     6901 tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt
     6961 gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt gaagtttttt
     7021 tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac tttatctgga
     7081 gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa ataaaggtaa
     7141 gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct
     7201 agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt gttcatctct
     7261 tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg
     7321 ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc aaatttttct
     7381 aatagttgac gtttattttt tatacttgat aatatatcag aatgattttc gtcgaatgac
     7441 tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc tatcggatta
     7501 ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag ttctcttaat
     7561 tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct aaggattgat
     7621 aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct aaaagtatag
     7681 aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc ttgttttctg
     7741 aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct tgttgaatca
     7801 ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc accacatata
     7861 atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt tcattgatat
     7921 caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga cttattccga
     7981 cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat actaccatgc
     8041 gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct gttatttcct
     8101 gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact tcgccaggca
     8161 atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc aattttagat
     8221 ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag cttgctccta
     8281 caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt ctactaccag
     8341 tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga tgatcaattc
     8401 cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct gcatttctct
     8461 tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact tttcctgtgc
     8521 tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct gtgctacttt
     8581 cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac
     8641 tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca ctgctcgtac
     8701 tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt gtgctatccg
     8761 tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc gtgatcgtca
     8821 tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc tcatcaatcg
     8881 atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt acgtctttta
     8941 aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca tatgttagaa
     9001 cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata gtaatgactt
     9061 gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga ttctcatttg
     9121 cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc agcccaagaa
     9181 taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata attaccttct
     9241 atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa
     9301 tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt aatcaatgac
     9361 agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat tgataaacag
     9421 cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca acaatcaaga
     9481 ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc ccagtaatta
     9541 ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg
     9601 gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca atagccgtaa
     9661 ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa aagatattga
     9721 aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat aatgcagcag
     9781 atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa aagccacgga
     9841 ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt atcagcggca
     9901 caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat ggactagtaa
     9961 taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc cagtcttttc
    10021 ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta gcaagaaata
    10081 aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca atccattcac
    10141 tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa acaaaaccaa
    10201 ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt catccctcct
    10261 ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt aaatgttctc
    10321 catcatagag agaatagatt tttacgaact aagaatatct acagcaacct tacgaaaatg
    10381 aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg atgggaaatt
    10441 ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta ctattctcag
    10501 gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt acagttcagg
    10561 atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa aaaatgattg
    10621 tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag
    10681 aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta aaagcagttg
    10741 gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc caatacggtt
    10801 tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa gaattctatc
    10861 cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat aaagcaaaaa
    10921 aagaagcaaa tgaaggggaa aatttctcca agcttgttca agcttatgga aaaaataaaa
    10981 cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag attcctactg
    11041 aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc attcctgtaa
    11101 cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa aaacaggata
    11161 aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc gaagcaaaaa
    11221 ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa
    11281 ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc caattgggga
    11341 tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag gcaaaccact
    11401 tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca ctggatttgt
    11461 tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga atcaatgcgc
    11521 cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc ttcaaaaaca
    11581 actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa aatatccctt
    11641 ctggacttgt tcctcttatt ctgttatact agagaaacac gaaagatatt tttatgcgac
    11701 acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga gttacaagga
    11761 tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt cacccaaact
    11821 ttaaattaat gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt
    11881 ttattttatg gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt
    11941 ttctgcttgg aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga
    12001 gggcatctct aatacttgat ctagtagctc tttacctaac gttatcgcat gagctccaga
    12061 tcgaatggct gtagtgactt gttcacaatt tttgaaacta gctgctaata tttttgttgg
    12121 tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc actttcatag catcaatgcc
    12181 ttgattttgc atcctgttat aataaggtgc aatataatct gctccattat taatagctaa
    12241 atacccttgt aattctgtgt aaatagctgt accggtaatc aaataacctt cttttttcaa
    12301 ctttttgatc gcttgaaggc ctgcttgatt caccggcacc ttgataaacg tatcttttcc
    12361 cagttcttct atgataactc gagcatcctc tatcatttct tcttccgttc ttcctattgt
    12421 ctgaatatgg ataggaatct gtccgattat ttttttgatt tctttcatat gcgagaagaa
    12481 gtttgacgct ccttcttttt tcagaattgt aggatttgtt gttacgcctg ttaaattcaa
    12541 aaactgttga caagaataaa tactttttgt atttgctgta tcaagcatga tttccatata
    12601 gctttccccc attctgatcc tcttgatcga ttttaatgat tgtccccttt cttaaatgcg
    12661 gcatttcctt atcttctaca tacattgttc ctggaagttc cggctcatga gatccatcaa
    12721 attttatcgt gatatgtcct aattcattca aattagtttg gacaacatct cctactgctg
    12781 tgattacata catttgttta tcaaaacaga gcttcatatc acaagttatt tcttttctta
    12841 cctgattcaa ttcaatctta taacaataat ctgttaatgt ttccggtgct tcatctccaa
    12901 ataaaatcat tattttttct ttcctgaata cttctgcttc tataccaaca tctgttacaa
    12961 ccgtttcaaa gatcgtcatc tctgttcctc ctcgtaaaat atcatttgtc taatagtttt
    13021 tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa cggaatttgt
    13081 ttcgatattg tgtgatttct cagagtacaa tggatgtatg ga
//
LOCUS       DARDHD010000071        13108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000071 DARDHD010000000
VERSION     DARDHD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.071"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..168
                     /locus_tag="SDA06_001562"
     CDS             1..168
                     /locus_tag="SDA06_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG4228839.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(230..2041)
                     /locus_tag="SDA06_001563"
     CDS             complement(230..2041)
                     /locus_tag="SDA06_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG4228840.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2038..2403)
                     /locus_tag="SDA06_001564"
     CDS             complement(2038..2403)
                     /locus_tag="SDA06_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG4228841.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2566..2961)
                     /locus_tag="SDA06_001565"
     CDS             complement(2566..2961)
                     /locus_tag="SDA06_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4228842.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2979..3941)
                     /locus_tag="SDA06_001566"
     CDS             complement(2979..3941)
                     /locus_tag="SDA06_001566"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG4228843.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3941..4153)
                     /locus_tag="SDA06_001567"
     CDS             complement(3941..4153)
                     /locus_tag="SDA06_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG4228844.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4174..4872)
                     /locus_tag="SDA06_001568"
     CDS             complement(4174..4872)
                     /locus_tag="SDA06_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG4228845.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4892..5434)
                     /locus_tag="SDA06_001569"
     CDS             complement(4892..5434)
                     /locus_tag="SDA06_001569"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG4228846.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5566..6570)
                     /locus_tag="SDA06_001570"
     CDS             complement(5566..6570)
                     /locus_tag="SDA06_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4228847.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6567..7556)
                     /locus_tag="SDA06_001571"
     CDS             complement(6567..7556)
                     /locus_tag="SDA06_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4228848.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7553..8359)
                     /locus_tag="SDA06_001572"
     CDS             complement(7553..8359)
                     /locus_tag="SDA06_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228849.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8525..9481
                     /locus_tag="SDA06_001573"
     CDS             8525..9481
                     /locus_tag="SDA06_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4228850.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9558..10076)
                     /locus_tag="SDA06_001574"
     CDS             complement(9558..10076)
                     /locus_tag="SDA06_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4228851.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10164..10313)
                     /gene="rpmG"
                     /locus_tag="SDA06_001575"
     CDS             complement(10164..10313)
                     /gene="rpmG"
                     /locus_tag="SDA06_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4228852.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10541..10987
                     /locus_tag="SDA06_001576"
     CDS             10541..10987
                     /locus_tag="SDA06_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG4228853.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11182..13077)
                     /gene="asnB"
                     /locus_tag="SDA06_001577"
     CDS             complement(11182..13077)
                     /gene="asnB"
                     /locus_tag="SDA06_001577"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG4228854.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4071 a         2790 c         2246 g         4001 t
ORIGIN      
        1 atgaaaaaat ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct
       61 ggtttggttg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg
      121 atcgaagaaa accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa
      181 aatacgcatg aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag
      241 tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc
      301 tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt
      361 cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag
      421 cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt
      481 ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag
      541 cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt
      601 gcttttgctg aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg
      661 ctgctaattc tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag
      721 taaaccatcc ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag
      781 cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg
      841 tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg
      901 aaaagggctc attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag
      961 ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct
     1021 caaaattttt ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca
     1081 actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat
     1141 gccattgctc caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct
     1201 tttgatttct tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg
     1261 gttccttgcc tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc
     1321 ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga
     1381 cattttcttt aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat
     1441 acagtaaaac atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat
     1501 tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct
     1561 gtaaaagata accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa
     1621 tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata
     1681 aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata attagttgct
     1741 tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca
     1801 agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca
     1861 cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac
     1921 ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat
     1981 ggagaatcct ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca
     2041 tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt
     2101 ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa
     2161 aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga
     2221 agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc
     2281 tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc
     2341 cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat
     2401 catagttgtt tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa
     2461 aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat
     2521 ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact
     2581 tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc
     2641 tgctttacgt aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata
     2701 gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt
     2761 tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg
     2821 tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc
     2881 cattactcgt agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat
     2941 attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat
     3001 gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat
     3061 tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat
     3121 tctcctgctg ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc
     3181 aagtttccgc aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac
     3241 ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct
     3301 agcattgctt tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt
     3361 accacacctg tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg
     3421 aacccttcag gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga
     3481 atcaaatttc cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca
     3541 acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg
     3601 tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga
     3661 acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt
     3721 cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa
     3781 cgatgattga ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg
     3841 gtatcgatgc tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct
     3901 gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc
     3961 ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat
     4021 gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat
     4081 agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat
     4141 ataagctctc catctttaag cacttccttc atattataca agtaaaccat atttttttaa
     4201 acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg
     4261 gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc
     4321 gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac
     4381 aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg
     4441 tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc
     4501 accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc
     4561 gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc
     4621 cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat
     4681 tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc
     4741 ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat
     4801 ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg
     4861 ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct
     4921 gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg
     4981 tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat
     5041 aaccggctct attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat
     5101 agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt
     5161 catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct
     5221 tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata
     5281 aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga
     5341 ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc
     5401 ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa
     5461 atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat
     5521 aaaaagaaga cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc
     5581 gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa
     5641 aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag
     5701 cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac
     5761 gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc
     5821 tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac
     5881 gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc
     5941 ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg
     6001 ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt
     6061 agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc
     6121 ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac
     6181 acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga
     6241 atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc
     6301 caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac
     6361 gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac
     6421 aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta
     6481 aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa
     6541 gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa
     6601 aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc
     6661 atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt
     6721 agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca
     6781 ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag
     6841 aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc
     6901 aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat
     6961 aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa
     7021 aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa
     7081 aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa
     7141 aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga
     7201 aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa
     7261 gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt caccccttgc
     7321 atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc
     7381 aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc
     7441 tgccaaaaat ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata
     7501 atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt
     7561 gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg
     7621 gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca
     7681 cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg
     7741 aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt
     7801 gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg
     7861 tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac
     7921 gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg
     7981 tagcatccaa cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt
     8041 tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt
     8101 cactagtttg tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga
     8161 tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt
     8221 tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag
     8281 gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag
     8341 ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg
     8401 ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca
     8461 gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa
     8521 agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt
     8581 actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac
     8641 ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa
     8701 cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa
     8761 acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt
     8821 aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc
     8881 tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag
     8941 caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact
     9001 aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta
     9061 tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc
     9121 tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg
     9181 aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa
     9241 aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga
     9301 ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa
     9361 gaatctaaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct
     9421 atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg
     9481 attttaacta aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca
     9541 aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat
     9601 ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata
     9661 agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg
     9721 atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca
     9781 cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa
     9841 tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata
     9901 tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa
     9961 attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac
    10021 aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta
    10081 tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca
    10141 aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg
    10201 cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt
    10261 agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc
    10321 cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat
    10381 tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat
    10441 acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg
    10501 tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga
    10561 ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc
    10621 aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca
    10681 agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa
    10741 aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc
    10801 agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa
    10861 ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca
    10921 aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga
    10981 ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga
    11041 aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag
    11101 agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt
    11161 tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat
    11221 cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt
    11281 tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg
    11341 atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg
    11401 ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca
    11461 ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt
    11521 caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg
    11581 tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt
    11641 tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc
    11701 tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg
    11761 tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc
    11821 ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc
    11881 gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat
    11941 cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc
    12001 atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa
    12061 gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg
    12121 gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag
    12181 ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga
    12241 gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa
    12301 gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc
    12361 gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc
    12421 ccaatattgc tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa
    12481 acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata
    12541 aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt
    12601 gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc
    12661 aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa
    12721 cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac
    12781 ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg
    12841 ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat
    12901 aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg
    12961 tttatcgata tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt
    13021 attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta
    13081 actttcacat cctaatataa aattactg
//
LOCUS       DARDHD010000072        12943 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000072 DARDHD010000000
VERSION     DARDHD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.072"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDA06_001578"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDA06_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG4228855.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SDA06_001579"
     CDS             complement(1195..3036)
                     /locus_tag="SDA06_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG4228856.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SDA06_001580"
     CDS             complement(3218..5533)
                     /locus_tag="SDA06_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4228857.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SDA06_001581"
     CDS             complement(5718..6578)
                     /locus_tag="SDA06_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG4228858.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SDA06_001582"
     CDS             complement(6637..7158)
                     /locus_tag="SDA06_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG4228859.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SDA06_001583"
     CDS             7436..8197
                     /locus_tag="SDA06_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4228860.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SDA06_001584"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SDA06_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4228861.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDA06_001585"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDA06_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4228862.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SDA06_001586"
     CDS             12557..12913
                     /locus_tag="SDA06_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4228863.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DARDHD010000073        12754 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000073 DARDHD010000000
VERSION     DARDHD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.073"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..2101
                     /gene="acm"
                     /locus_tag="SDA06_001587"
     CDS             <1..2101
                     /gene="acm"
                     /locus_tag="SDA06_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG4228864.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     LIVTICAGIFLQKYKKV"
     gene            complement(2373..2858)
                     /locus_tag="SDA06_001588"
     CDS             complement(2373..2858)
                     /locus_tag="SDA06_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG4228865.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2948..3293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3338..3979
                     /locus_tag="SDA06_001589"
     CDS             3338..3979
                     /locus_tag="SDA06_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4228866.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            3998..4633
                     /locus_tag="SDA06_001590"
     CDS             3998..4633
                     /locus_tag="SDA06_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4228867.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4651..5466
                     /locus_tag="SDA06_001591"
     CDS             4651..5466
                     /locus_tag="SDA06_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228868.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5785..7221
                     /locus_tag="SDA06_001592"
     CDS             5785..7221
                     /locus_tag="SDA06_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4228869.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7410..9857
                     /locus_tag="SDA06_001593"
     CDS             7410..9857
                     /locus_tag="SDA06_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG4228870.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10087..11154
                     /gene="rlmN"
                     /locus_tag="SDA06_001594"
     CDS             10087..11154
                     /gene="rlmN"
                     /locus_tag="SDA06_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG4228871.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11347..12450)
                     /locus_tag="SDA06_001595"
     CDS             complement(11347..12450)
                     /locus_tag="SDA06_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4228872.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4350 a         2155 c         2709 g         3540 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta
     2101 gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt
     2161 ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg
     2221 gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac
     2281 attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc
     2341 ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt
     2401 tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg
     2461 atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg
     2521 atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct
     2581 aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca
     2641 gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt
     2701 ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca
     2761 tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt
     2821 agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt
     2881 attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa
     2941 gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg
     3001 ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg
     3061 tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa
     3121 ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt
     3181 gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt
     3241 ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg
     3301 ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat
     3361 gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg
     3421 ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg
     3481 ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat
     3541 ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact
     3601 cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca
     3661 atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac
     3721 ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga
     3781 agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat
     3841 gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc
     3901 aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa
     3961 attaaattat taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa
     4021 caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa
     4081 aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc
     4141 tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt
     4201 cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc
     4261 acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa
     4321 agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt
     4381 gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc
     4441 attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga
     4501 attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat
     4561 tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc
     4621 cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg
     4681 atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg
     4741 gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg
     4801 ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc
     4861 tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt
     4921 gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga
     4981 gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct
     5041 aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat
     5101 gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa
     5161 aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga
     5221 cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac
     5281 gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt
     5341 gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag
     5401 aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga
     5461 aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca
     5521 gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt
     5581 aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa
     5641 gccattttca caaaattttt cttactactc gaaactaagc gactttttca caacctcctt
     5701 tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga
     5761 aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat
     5821 tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg
     5881 aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg
     5941 aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa
     6001 gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga
     6061 cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt
     6121 gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa
     6181 gatgaatata atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa
     6241 acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat
     6301 atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa
     6361 cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt
     6421 cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca
     6481 attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat
     6541 tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa
     6601 gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa
     6661 ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac
     6721 ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga
     6781 gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg
     6841 gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg
     6901 ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa
     6961 atccatgacg actatcgaat caattactta caaagccacg tcaaagccat caaagaagca
     7021 atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc
     7081 tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa
     7141 gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa
     7201 gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc
     7261 cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa
     7321 atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata
     7381 atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa
     7441 ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg
     7501 gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag
     7561 gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc
     7621 ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta
     7681 aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg
     7741 cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt
     7801 tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg
     7861 gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag
     7921 cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt
     7981 taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca
     8041 agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag
     8101 aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga
     8161 aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata
     8221 cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg
     8281 cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt
     8341 cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg
     8401 aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc
     8461 aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa
     8521 gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg
     8581 aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg
     8641 cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga
     8701 ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag
     8761 cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac
     8821 gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta
     8881 gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc
     8941 atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca
     9001 tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa
     9061 aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata
     9121 tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa
     9181 ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca
     9241 atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata
     9301 tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg
     9361 taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt
     9421 ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga
     9481 ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc
     9541 aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag
     9601 ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac
     9661 ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt
     9721 acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag
     9781 aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg
     9841 acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa
     9901 gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta
     9961 tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt
    10021 attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa
    10081 aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt
    10141 atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa
    10201 cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg
    10261 gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca
    10321 gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa
    10381 gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt
    10441 tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag
    10501 atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc
    10561 gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat
    10621 atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt
    10681 ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt
    10741 tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc
    10801 ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc
    10861 acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta
    10921 gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca
    10981 gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat
    11041 gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat
    11101 gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat
    11161 cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa
    11221 cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac
    11281 cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc
    11341 aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc
    11401 tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat
    11461 atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt
    11521 catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa
    11581 caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc
    11641 gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat
    11701 agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc
    11761 cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg
    11821 cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc
    11881 cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc
    11941 atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat
    12001 gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt
    12061 atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa
    12121 gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc
    12181 gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt
    12241 tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa
    12301 agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac
    12361 ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt
    12421 ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg
    12481 gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct
    12541 aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc
    12601 taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag
    12661 tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt
    12721 attatatgaa aacaggtttt atttgtcaat tcat
//
LOCUS       DARDHD010000074        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000074 DARDHD010000000
VERSION     DARDHD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.074"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SDA06_001596"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SDA06_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4228873.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SDA06_001597"
     CDS             complement(322..891)
                     /locus_tag="SDA06_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG4228874.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SDA06_001598"
     CDS             1077..1979
                     /locus_tag="SDA06_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4228875.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SDA06_001599"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SDA06_001599"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG4228876.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SDA06_001600"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SDA06_001600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG4228877.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SDA06_001601"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SDA06_001601"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG4228878.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SDA06_001602"
     CDS             8547..8732
                     /locus_tag="SDA06_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG4228879.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SDA06_001603"
     CDS             complement(8794..9990)
                     /locus_tag="SDA06_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4228880.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SDA06_001604"
     CDS             complement(10160..11596)
                     /locus_tag="SDA06_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4228881.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SDA06_001605"
     CDS             11889..12698
                     /locus_tag="SDA06_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4228882.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARDHD010000075        12541 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000075 DARDHD010000000
VERSION     DARDHD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.075"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..243
                     /locus_tag="SDA06_001606"
     CDS             <1..243
                     /locus_tag="SDA06_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228883.1"
                     /translation="RYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKL
                     AIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(388..1128)
                     /gene="truA"
                     /locus_tag="SDA06_001607"
     CDS             complement(388..1128)
                     /gene="truA"
                     /locus_tag="SDA06_001607"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4228884.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1319..1954)
                     /locus_tag="SDA06_001608"
     CDS             complement(1319..1954)
                     /locus_tag="SDA06_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4228885.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(2101..3342)
                     /gene="sstT"
                     /locus_tag="SDA06_001609"
     CDS             complement(2101..3342)
                     /gene="sstT"
                     /locus_tag="SDA06_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG4228886.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3540..4538
                     /locus_tag="SDA06_001610"
     CDS             3540..4538
                     /locus_tag="SDA06_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228887.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4551..6569
                     /locus_tag="SDA06_001611"
     CDS             4551..6569
                     /locus_tag="SDA06_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG4228888.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6629..7345)
                     /locus_tag="SDA06_001612"
     CDS             complement(6629..7345)
                     /locus_tag="SDA06_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG4228889.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7572..8114)
                     /locus_tag="SDA06_001613"
     CDS             complement(7572..8114)
                     /locus_tag="SDA06_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4228890.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8119..8346)
                     /locus_tag="SDA06_001614"
     CDS             complement(8119..8346)
                     /locus_tag="SDA06_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4228891.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8425..10305)
                     /locus_tag="SDA06_001615"
     CDS             complement(8425..10305)
                     /locus_tag="SDA06_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4228892.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10494..11108)
                     /locus_tag="SDA06_001616"
     CDS             complement(10494..11108)
                     /locus_tag="SDA06_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228893.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11250..11810
                     /locus_tag="SDA06_001617"
     CDS             11250..11810
                     /locus_tag="SDA06_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4228894.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12115..12429)
                     /locus_tag="SDA06_001618"
                     /pseudo
     CDS             complement(12115..12429)
                     /locus_tag="SDA06_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         3917 a         2484 c         1998 g         4142 t
ORIGIN      
        1 cgttataaag ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag
       61 tttggcacaa ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag
      121 tacacaaaac tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta
      181 ctttatcgat ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac
      241 tagattttta tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt
      301 tttatatttc tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca
      361 tgcgtttcat ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc
      421 ttgtgcaggg acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat
      481 tgtttctatc ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac
      541 cattttatat aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc
      601 gatagataat tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa
      661 accaatgaaa tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc
      721 caactcttga gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt
      781 ccaaacataa tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg
      841 catttcttcg attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat
      901 catttctgcc agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg
      961 ggtgccagca tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg
     1021 caatactttt tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc
     1081 tgaatatctt tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct
     1141 cctctgtctt ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct
     1201 ccttgcttct ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa
     1261 acaaaaaaca gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt
     1321 actgtttgag atgcaagata ctcaacctgg caatgggcac ataattaaag aaaaaactga
     1381 ttctttggca aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg
     1441 ccagtccata aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc
     1501 ttacgtccat ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa
     1561 aaccactgta acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag
     1621 attcatgggt attgatactg aacaaacctg taaagataca atcacctata ccatagaacg
     1681 cgatggctgc tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca
     1741 atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt
     1801 ttcttacagg actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt
     1861 caggataata caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata
     1921 aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta
     1981 tgcttttaat tgttattata aacgacttta tcttgattta acagaaacct ctatttacac
     2041 gaacatctat ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt
     2101 tcaacgttca cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt
     2161 acgcgcgcct agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt
     2221 ttccattgaa tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac
     2281 atcattagag ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc
     2341 aacacctgaa gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac
     2401 aaaaggtgct tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat
     2461 cgcggcaccg cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt
     2521 tgtcaatttc aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg
     2581 ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt
     2641 ttctttaata aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt
     2701 accgatcaaa acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc
     2761 aattgattga taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac
     2821 gacttgtgac aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc
     2881 tcgaaaagct agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat
     2941 aagagattga acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa
     3001 actttgcggt gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga
     3061 acttacaata actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat
     3121 aggcttcacg aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa
     3181 aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga
     3241 aataaatgtc catgtgggga aaaagatacc aaacaatatt ccgactaaaa taccaacaac
     3301 aatacgttga actaacgaca tatccaaaaa cttctctaac aaatgaccac cctttcacaa
     3361 ttttaacaat cattgtatca ttttatgcaa agaaactaaa ggtataattt tctaaaaatt
     3421 ctaacaatac aaacaaccat ttcttgctaa ttttgtactt tttctgtcta cacgttgttt
     3481 tttgagatat tctaagttag gatgttatta atgggcaact tatcaggaaa cgttttctta
     3541 tgtttttgtc caattttttt gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt
     3601 atggcttcct gacaatcttt gtcgcttgta taatgatggt tttacagcgt atattgattt
     3661 cagcagtcac tattcctaca tatgcaacaa accctggtcg attctacatc tatacatttt
     3721 tacaaacttc cgttgttatt ggtgcaaatt tgatcccact ttacatgggt taccaatatc
     3781 aaaaaatcgc ccgtttaaaa gttttacatt atttaagtcg ttttgccttt atattcctaa
     3841 tagccacact agtttgcaat atcttttttt acgtacaagc tggcagcttg aatattcgtg
     3901 attattggct tatcctaaca cctatcagtc agaattactt tacttatgct gtttcttgta
     3961 ctttgctttt ttgtagtatt ccttatacag ttggattttt aaataaatta tcgaaaacaa
     4021 caattaaata tctactatta tttttaacaa ttggattagt tggctgctct actctattta
     4081 ataaagaccc gtttgcatta cagaatggac agagtatttt atggattttt tatttatttt
     4141 taatgggtta tggtttgaaa gaatggaact ggaaagaaaa ggtccgctat aagcttcctc
     4201 aagtatttat cagcttgatt gttttattcg gtttgattat tttgatgaca caaatttcac
     4261 tcatcatcag aggaaataca gatacagcgc tgaggttttc taaaccatat tctctcttcg
     4321 caatgtatta tactttctct tcgtttttat ttttagaaat gctatctgat aaattaggta
     4381 taaaaggaag aggaaatttt cttgctgtat ttttgattgt cactcaagtt atatgtaatt
     4441 tccctttagt ttcttatcgc gtatctacat ttatgaaacg tgaatttcca aactcaggga
     4501 aggcatggct gataaatatc gggcagtttt ttatttgata tttattagca gtgtccctcc
     4561 tagttatctt tttattttat atacaaaaaa tccgtttcat tcatcgtttc atcgaatact
     4621 tcgcttttga ttcgattgaa caattaactc agcgttggaa aaaaagaatt cattggcttt
     4681 ttgttcataa aagttatttt ttagttgcag cattttttta ctgctttacc ttcgtgcaga
     4741 ttttcgtttt agaaccaaaa gaaggttgga aagaaactat ccaggttgcc ttaaaaatat
     4801 ttactcagcg acaagcaccc atgatcctga caatcatcat tattatggga tttttcttct
     4861 tactattgtt gatttccaat cgtttttggt acgccttgac tgctaccttg atcattaact
     4921 tactgctcac tatttcgaca gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt
     4981 cagatttaaa aatgctgacc ggattgtcag aactattatc gatggttagc ccagtcttat
     5041 taatcgtggg cggattgatc cttctcctat tattgattac tagtattatt gtacaacgta
     5101 gactacaaaa acagtattcc ttgaagattc attggaaacg tcgatttatc agtatcgctg
     5161 tattattagg atgtttttcg ggtgtctttt tcattaatca caaaaattcg ccttctttct
     5221 tgctgtttaa tttatttaaa gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag
     5281 ataacggacc agtcattcaa ttcctaaaca atatcgacat taaaatcatg gaagaacctg
     5341 ctggctattc gaaagaaaaa attaatggaa taatgaaaaa gtacaacaca aaggcagatg
     5401 aaatcaacca aacaagaagt gactggttaa acaatcagac gatcgttctc aatttaagtg
     5461 aaagcttcag tgatcctgca cgtgtcccta atctgacagt acctacagac cctattcctt
     5521 acatcaaaag cattataaca gaaggaacag gaggattgat gttatccgtc ggttatggtg
     5581 gcggaacagc taacatggaa tgggaaggac tgacaggctt gagtatcagt aacctgtctg
     5641 cttcgttagt tacaccttat acccagctag tggagcgtca gaaaataagt ccaaatatta
     5701 ccgatctatt cgatgaaaaa atagccatcc atccttttac agctgctctt tatcgcagaa
     5761 aacaagtgtt tgataaattt ggttttgaca ggttctatta tttagacagt ccggataaat
     5821 taacttatac ggataaaata gaacaaagcc cacgcatatc ggatgattca gcatacaaag
     5881 aaaccttgaa agctttacaa agcaatacgg aaacgtcaca attcatccaa ttatccacta
     5941 tgcaaaacca tatgccttat gatgattact atagtgaatt gaactacact gctgaaggaa
     6001 ctgctgtcat tcctaataga agaaaagaat tacaaactta tatgcaaggc cttcactata
     6061 ccgatacagc agtaaaaaaa ttcatccaag aaatcgacaa aatcgataaa ccgattacct
     6121 ttgtttttta tggtgaccat ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg
     6181 gattagaaca gcaccagaca gattatttca tttataacaa caagtatagt cgtgaacaag
     6241 cagctacgtt aaataaagaa gttgttgctc ctaataactt tggtgctatg gctcttgagc
     6301 atgcgaatat caaagtaact ccttactatg cgttgttgac agaagtatct aatcatctac
     6361 ctgcattaac aattgatcct acagaaagct tgtctaatcg ttttaatgga aaacaggtct
     6421 ttgttacaga ggatagtcaa attatccaag aaaaaaattt aactgagaaa caaaaagaaa
     6481 ttctagaaga ttatcgtctg atacaatatg atcttgttgc aggtaaccag tacagtgcaa
     6541 catgggcgac acaaaaaata aaagactaac aaataaactg agcaaggggt tgtgataaaa
     6601 gacatgtcac aaccccttgt ttatctcatt aatttgcttg tccataaacg atttgattaa
     6661 agccattttt caattcatta ttcgctgtct cgttgaagta aagataatta tcgggattcc
     6721 cctgattttg ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga
     6781 cgtgcttttt cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt
     6841 ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca
     6901 tcttccactc cccgttcaaa atagcttgag ggactttaag accatataag tccacactca
     6961 tttgattcgt ataacttttg tattgttgtc ccattgtttg gccccatttg gtcataaatg
     7021 cctctaattg tgcggatttg tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt
     7081 ctgaagagga agatgaagcc tctacagaag aacttgaagt agatgtttga gttgtttcag
     7141 tcgttttgga agactctgac gaagtagagg tatctgctgt ttctgtactt gctgcagttg
     7201 acttgctctc tgtgctagtt gttgtagtag ttgcaactgt tgctttcgct gtttcttttg
     7261 ttgattcttt tttcgattca ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa
     7321 ttgttactaa aatccttttt ttcaattaaa aaactccttt cgattactcc cacttcttta
     7381 ttctatcaaa taagcaatgt gattttattg atttttcgta ttttctcatc aatctttcaa
     7441 atcttctaaa aactgagaag cacccgctga tgttgtctgt aatacatcag cgggtgcttc
     7501 ttttatttta atcttcgtga ttttccaatt tacctcacgc tttatatttg tatttgaaga
     7561 aacatagact tttagtcatc atcgtcttcc tcatccaaat cttgtcggac agtttttcct
     7621 tggagtcttt gtacttggta gatgatttct ttattgaaac ttaaaagttc aacaagcaat
     7681 tgttgcaagc catatttttc ttctttttca gctaatttga ttgcccgtgt gataaagaca
     7741 ttttggaaat caaaatcttt tgcaaagttt tccaacattg ttgaggctgg taaatatttg
     7801 ttttgaccat gttcgtaaat ttttccataa cgcatgactt cttctaaagt agacggaacg
     7861 atttcatttt caatcactaa ctcatgagag attttattga accatctcgc ttcttgatca
     7921 gcatattctg aaaaaagggt acttaattct tgcgaagatg tacccaaaac atacaattgt
     7981 gcttggatca atttcatttc ttctaattta aaatttgcta atacatgccc caccatggcg
     8041 ccagcagttg gtttatgatg gtcgatatct gcttgttttg tttcttgaac aaatttttct
     8101 tccactatat tcattgattt acgcctcttt tacttttgct gattggactt cataacctaa
     8161 tttttccaca actgcagcta attgttcagg gtggacttca tctggatcaa agtttgcttt
     8221 gattttgctc gcattaaaca aaactttcgt attgcttacc ccaggctgtt gagaaaccgc
     8281 atgttcgatt ttttgcatac agcttggaca agctagtgta tctaattgca taataatttt
     8341 tttcatcgta gttcttcctt cctttttctt gatagtctta ttctagatta cagcttccat
     8401 aaaaaaattg attcttatca agttttacga tttttcttct ttgctttcgt gtccaaaata
     8461 caataaacgc atcgcgttaa agataacgat caagatacta gcttcgtgaa cgaacatccc
     8521 actagccatg taaataaacc cggcaaataa gccgttgagt aaaaataaga cgacaccaat
     8581 ggcaatcact acattttcac gggtatttaa aacagttttc ttagccaagc ggtaggcatg
     8641 aaccaacgat tcaaaattag attgcatcaa gacaacgtca ctcgtttcga tagcaacatc
     8701 cgttcctgat cccatagcaa tcccgatatc agccgcagcc aaagagggag aatcattgat
     8761 tccatctcca ataaaagcaa cgcgcaatcc ttgttcttgg tattttttca caaattgaac
     8821 cttttcgtct ggtaataatt cagcatggac ttcatcaatg cctaacatat cagctacata
     8881 atcagcagtc atctgattat cacctgtcag catcactaac tgttttgctc ctgcttgttt
     8941 caacttagct aattgttcag cgacttctgg tcgaatcaca tctgacacac ctatgatttg
     9001 agtgattttt ttgtcgattg ctacaataat gaccgagctg cctgtttgtt gtaattgatg
     9061 taaatcttgt acttgctttg gtgtgagaga aagtttgtga gcggcaatca atttggtgtt
     9121 gcccgcacaa actgtatgac catcaatctc agctgtgatt ccttgtccct tgattgtctg
     9181 attatctttg actgctaatt gttgaaaatc aatgtctttc tcctgagcat aatggatcac
     9241 ggcgcgcccc agaggatgat cggataacgt ttctaatcga gccactaaag ctaagctgtc
     9301 cccttcatcc tctgtatagt tcttgatagt ggatacagca gtagttcctt tggtcaatgt
     9361 accagtttta tcaaagacaa aagtatcgat tttggaaaaa gtatccattg cttcaccgcc
     9421 tttgactaag atgccccgtt tcgctccatt accgattcct gctacattcg aaacaggcgc
     9481 accaatgact aaagcgccag gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc
     9541 acgactaatc agaccaacaa ccaacgcaat aagtaaaacg gttggagtat aatactgaga
     9601 aaatcgatca atgaattttt ccgcatggga tttggtatct tgtgcttctt ctactaattc
     9661 gataattttg gcaaaggtcg tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc
     9721 atctgctaaa atcgttccag agaagacttc atctccactg attttgacga cttgtcgtga
     9781 ttcaccagtc actgcggctt catccgcata ccctttcccg tcaatgactc gtccgtccac
     9841 aggaatagct ccaccagttt ttactaagac gatatcgcct tcttccacgt catcgatttc
     9901 cacttcttca gtcgttccat cttcttggac aacactagca gtagatgggg ccatctcggt
     9961 caaagccttg attgactccc gcgttttggt caatgttttt tgttccagat agctaccaaa
    10021 caaaaacaag aatgtaacga tagctgactc cttgtattct ccgatcataa aggcaccaag
    10081 cacagcaata gtaacgagta aatcaattga gataactttg actttcactg cctgatacgc
    10141 atggatcata ataggtatga ccgcaataat agaagctatg gctagagcaa tgttatagaa
    10201 caagacaaaa ccagtaaatt ttccgataaa accaaaaata atcaacacag ctgataaggc
    10261 ggtgatatga tttcgatgct cttggatcca cttttgtaat ttcatctttc tccctctttt
    10321 cctttcattc ttacgagact aagtatagat acttatccat cataaaaaat tgaccactat
    10381 caagtttagt caaaaaggcg gttttctttt ggtagaatac actacaaaaa atagttagca
    10441 agaagactca ctaaaagtga tcttgccaac tattgtcgtt tctatcgaaa ttttcattga
    10501 atgattttcc tgtacagcat acatcggaga tctatttctt tatctaagat ttttatcttg
    10561 ctaatttttt cctttagtct ttcaaatcca cgtcgttcat agaattgata cgtacagcca
    10621 ctatctgtaa aaaggaatat ttcttttcct ttctctcttt tttcaaattc tgttagcaac
    10681 ttggtaccga tgccttttgt tttcaaatca ggatttgcag ctaaaaaaat gatctctcca
    10741 tctggcgaat gcttctttaa atatgctgca agcatttgtt catttgctgt ctcgtaagct
    10801 cctttacttt ctttagcaaa tatattttgt aaaaaatcga atatcttaac atatatttgc
    10861 tgtccaaaag aacgatgttt ctttttttct ccttttatct ccgccagcaa aacacctgcc
    10921 aactcgtcct ctgcatacaa agcaagaatt tgagttgctc gattcaactc catatacaaa
    10981 aaatatcttc cgtaaagatt cagcagaagt tcattttcca tatatctctc aaagttcatt
    11041 ccttcaacag caaaatagat agcttttttg taatctttct tttctaaggg tttaaaagtt
    11101 agtttcatca tttcgcctcc tcaacttcaa tgattgtgtt atacttacat tataatagac
    11161 acagtgtcta ctatctataa tcaatcatct ataagtttgt cgattaagcg acagctttgc
    11221 tttgatatgt cggataagga gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa
    11281 cagaaaaagc tttggctgaa acactatcca cattattagt aaataaaaag attcaagcaa
    11341 tcactatacg cgaactaaca gaaacggcag atgtacatcg ttcgactttt tatacacatt
    11401 ataaggatat ctatgatcta tatgatcaat tagaaagcaa cttttttcgg gatctaaatg
    11461 aaattctctc ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact
    11521 atgtttacgc aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt
    11581 ttcagaagaa agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag
    11641 acaagaaaac gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt
    11701 gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat
    11761 tagaactctt acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat
    11821 actaagctta gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc
    11881 aaattgttta ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta
    11941 gtttgtagtg ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac
    12001 ctcatcatta ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa
    12061 ccaatttata gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct
    12121 caattcattt tcaaggggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat
    12181 ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg gctttatatc cttcgtaatc
    12241 cttactgttg ataattgctt tcatattagg aattggatct tcacctggca tatattgcag
    12301 attagaagca tgtaattttt tctcagaaac aaaccactca ttggaaaaga catgaatcaa
    12361 atcatcactt acttgtgtaa aatagcgacg tttaatgaca aagatatctt cattgctatc
    12421 tagtgtacct tcatttcttt gtgcttaaat tcaaagataa ttttctcaat aaagattttt
    12481 gattccagta agattccttt actcctttta caacatagcg ttgtcctttg ttttgaaaca
    12541 t
//
LOCUS       DARDHD010000076        12522 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000076 DARDHD010000000
VERSION     DARDHD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.076"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(96..665)
                     /locus_tag="SDA06_001619"
     CDS             complement(96..665)
                     /locus_tag="SDA06_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4228895.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(739..1062)
                     /locus_tag="SDA06_001620"
     CDS             complement(739..1062)
                     /locus_tag="SDA06_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228896.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1216..2028)
                     /locus_tag="SDA06_001621"
     CDS             complement(1216..2028)
                     /locus_tag="SDA06_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228897.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            2382..3230
                     /locus_tag="SDA06_001622"
     CDS             2382..3230
                     /locus_tag="SDA06_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG4228898.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            3375..4316
                     /locus_tag="SDA06_001623"
     CDS             3375..4316
                     /locus_tag="SDA06_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG4228899.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            4306..6477
                     /locus_tag="SDA06_001624"
                     /pseudo
     CDS             4306..6477
                     /locus_tag="SDA06_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(6589..8760)
                     /gene="copB"
                     /locus_tag="SDA06_001625"
     CDS             complement(6589..8760)
                     /gene="copB"
                     /locus_tag="SDA06_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG4228900.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(8780..10966)
                     /locus_tag="SDA06_001626"
     CDS             complement(8780..10966)
                     /locus_tag="SDA06_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG4228901.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(10966..11175)
                     /gene="copZ"
                     /locus_tag="SDA06_001627"
     CDS             complement(10966..11175)
                     /gene="copZ"
                     /locus_tag="SDA06_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG4228902.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11188..11628)
                     /locus_tag="SDA06_001628"
     CDS             complement(11188..11628)
                     /locus_tag="SDA06_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG4228903.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11702..12241)
                     /locus_tag="SDA06_001629"
     CDS             complement(11702..12241)
                     /locus_tag="SDA06_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4228904.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3516 a         2635 c         2231 g         4140 t
ORIGIN      
        1 aatctagcga aaaaccctta tcttaaaggc gttcgttcaa ttctttggca aggtcttcaa
       61 agcctggctt gcctagaagt gcgaacatgg ttttcttata gctcatgtag aaaaattttc
      121 ttgatgatga ttttatccat caattgagat tgactgtttt gctctcctat gatttcagct
      181 atgattgtcc gattttgcgg ttttcttctt aactggtagt tgattttccc agtttccatc
      241 aactcgctaa gcatatccac gactccgcca aatccggtct ttttcgtaat aaaacctagt
      301 gctaccattc gttcatctag attttttgct gtaatcgtca caccagtaat gagcgagtgc
      361 tgtaatgttt gggccagctc ttccatccct tcagcattgg atacgacgga agctgttttg
      421 ttaataggtt cttcgtttat ttcttttggt tctttcctct ccactttcac tgtttgacga
      481 aggacatcta gttcttgcaa gagcgtttcg tattgttctt gggtcagcat cacgccagct
      541 actttttcac gattaaatat atatacgcca gttcccgcgt ctcgcgcttg agcaaataca
      601 tccatcggcg atcgtttcac ttctgtgatc gaagatgtgg gaacttctaa tttctttaat
      661 tccaatggat ttcctccttt actaatcgct ttttattata gcacagactg acttgttttg
      721 atcttttttc caaaggtttt atcggttttt gcctaaccag taattgatat cggtaagaag
      781 tctttcgata ccaaatgaag gatggtacag atacgttttt tgatcttcgt tatggtctag
      841 tggaaaaaaa tgatcgatga tcactagatc tgcataccga ggcggcttat ttcgagtaca
      901 tacatgtata gagatccgtt ttcgatgtct tagcgttttg ttcagtaccc cagcaagtct
      961 tcttgcttct tggtgttcat agcgaaaaaa aaagacgatt ttcacttcag gagggtggac
     1021 atctaaatac cctcgttcct ggagtgtttt cagtaaaagc atcccccact ctactgcggg
     1081 gtgtgcaagt gcttgttgga tcgctagccg aaattgatat agtttatctt tggaattggg
     1141 aaatttttct aatgtttcct gataataacg aacttcttct atcagccggt catggatacc
     1201 aattacgtaa cgaagtcatt cttggtaaag tacttttgtc agctttgatt tgctttcgct
     1261 ctcaaattcg ctattcagcc aaaacagcga atcaagcttt tcagtcacaa tcgtcaattt
     1321 ccgatagtct tgtgtctctt ctatccgttt ccaccgcttt tcagtaatct ggttacagct
     1381 caagcgcaaa attgctagat aatttttttc ttttattggc aaaatctgct cctctctaaa
     1441 tagcaattca ggaaacaatt caaagaatag tgttcgactg tctcctaaaa tctcgatttc
     1501 tgccggatcc tttctttgct tcaataattg gatgaacatc tcagaaaagg actgctctcc
     1561 atcatctgca aaaaaatgcg gaaaatcttt caaaaagcaa aacagcagat atcttacttt
     1621 tctttctgaa ccaaagatct gtgctttttt ataagaaaaa gaaaggtggt actcgccaaa
     1681 ccactttctc aatcgctctt gcgtagaacg atcgtacttc tgctcgtaag gttcattcaa
     1741 tagcagattt ttcagtaaac aaaacggggg tgattggatc agaaaaacaa acaagagata
     1801 aggttcgcta tatccttgat cataaactaa ctgaacggtt ttcttatgaa gaatcagctg
     1861 aatgggacag tcgctttccg ccaaacgttt cagatgccga tccactgttt taacatcgat
     1921 tttcagttct tcagctaaat cagcttttcg ccagatcaaa tctttcatta gattgattag
     1981 tgaaaataaa ttctgtgcat tttttgataa tagtttttgc catctcatcg atcatttctc
     2041 acctgatttc taaacatatt gtttgatcaa aatgggttca ctcctaaaaa gataaaaaac
     2101 tccctttcta tttatttaaa tacgcaaatc ttcctcatta ttttttattt tactcaattt
     2161 aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa cataataaga tcactttgct
     2221 tgtatgatgt attcccttcc attacaatga aataaatagc gattttatga gaaggtgatg
     2281 tgaaaatgga agaaaaaata gaagaatcca aacaaaaaat cgatcagttg atataagaat
     2341 tagaaaaagc ggtcatcagc aaacgttcaa ctatccaatt gatgatcgtg gccctattag
     2401 caggtggtca tgtcttattc gaagatattc ccggtgtagg taaaacgtta ctggtcaaag
     2461 cactagcaaa agctgtacag ggaaccttca gcagagtcca atgtactcct gatctgctgc
     2521 ctagtgatat tcttggtttt tccgtataca attcacaatc aaaagagttt gaatttcgtc
     2581 cgggaccgat ctttaccacg atcttgttag cagatgagat caatcgtacg actcctcgta
     2641 ctcagtcagc acttttagaa gcaatggccg agaatcatgc aacgatcgac aacaatacat
     2701 accctctaga caatcatttt tttgtcttag ctacgcaaaa tccaatcgag tacgaaggaa
     2761 catatccttt gccggaagca cagctcgatc gttttttatt ccgactacaa atcggttatc
     2821 cttcttttga tgacgaactt ttgctacttt tggataaatt cgaaaaagtg ttggataact
     2881 taaatgaagt attgaacagt tatgaaatag aagaaataaa aacaaatgtg gcgaatgtct
     2941 ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt gtcagcaaca agaagtcatg
     3001 ctgccatcca gcttggcatc agtccacgag gaagcctttc ctttattcag gcagcaaaag
     3061 cttatgcttt aatccatggg agaaattacg tcacgcccaa agatttgcag gatttagttc
     3121 cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc attgaaggaa gacgaaaaga
     3181 aacaaatcat tcatactctt gttgcacaaa ttcctattcc tgtgaggtag attgaggtag
     3241 acaatggaaa aaatcaaaga ttatttcatt aattttgtgt tatttctggt atatttcatc
     3301 attgtgcttt ttgctcttgt attcaacaat tctttaggct ggttcttgtt ggcctttttt
     3361 actctttatg tatcatggag cttgatttca ttaagcagct ttctaaagaa actttcggtt
     3421 acttgtacat tgcagccact cgtttatcgc aaaaaaagta tgcaggttat tttttccata
     3481 gcaaaaaaga gaactttttc tttacctctc cctcgtttac aaatcggctt cccagcaatc
     3541 tttgcagaaa aaaacaagag attttgattc ttacaaataa accgcaagaa gtccaattgc
     3601 tttggcagcc aaaagaaaga ggatgccatg aaacattaac tgtcattttt acagcctatg
     3661 atagtctagg acttttcaga aaaagtgcaa aaagagagat ctcttttcct gtgactgttc
     3721 tgcctgcttt ttgtaagatt caagcagaaa aattacagag agttctggaa aaaaaacagc
     3781 agctgaacgc gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg
     3841 attctttcaa ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg ttataccgtg
     3901 aatacgatta tcaagaagaa gaatgttctc ctcttttttg cttttggggt ttgcccagtc
     3961 caaaatttga acctctgcta gacttgtatt tttctttatg gcatttgaga aaagaaaagc
     4021 agccggaact tctgatatta ggcaaccgcg cctttcatgg caaagacccc ggtcatacct
     4081 attttgcttc tttggaagct ggcgatgaga aagctttttt cgcacacttg aaaaaatatg
     4141 caaaaaaaca aatcgtcctc tttgttcctg agaattctcc tgaagtctca gaagctattg
     4201 aaacattgtc aaaagatcga acggtttatc ttgtttattt cgtagaaaaa caactcatgg
     4261 tccaagaaaa agacaagata tgttcattgc caggaggtga atggattgac gattaaacgg
     4321 gaaaaatggc tgcgtggcgt ttactccttt gtgatactaa gtgtaggtgc tccgatattt
     4381 gtagtagcta atcaattgca aaacagctac cttcttatcc taactattgg tgtcctatgc
     4441 cttattgtct gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg
     4501 ataggttgtt tgtttttata ttttccttcg aagacatttt ccatattatg gatcttacag
     4561 tttttttcgt cagtcacaga ggaattcttc catctatccc aaggtaaact ttattatttg
     4621 ccagaaaaga cagctttttt tctcataatg ttgctgatct atttatttat tacattgacc
     4681 gtggattacg actattggta cgcacccttt ctttctttga tggcttactt catgatgctt
     4741 accgtttttc ggaaaaaaga tttactgtac gaaatgattg cggtagtaac tgttttgttc
     4801 gtgtatctgg cagcaaggca atttttctcc atccggttgt tgtcgggaaa cactcgattc
     4861 cagtcattga ctacagttct gcttatttgt ttcttaactt ttagcgctat cttgccttta
     4921 tatcttccga aagtacacca atctctcgag gaaacgagtg aaccgatccg agaatatctt
     4981 aatgatgaag ggctttatga cacactccat gagtatcaat taaccggaag tgcaagaaca
     5041 ggcttcagtg agaatgatga acagcttggt ggacctttat atgataatgg cgaagttgtt
     5101 tttacagcga accaaaaagg aaatggctat tggaagatcg aaaataaaaa tcattacacc
     5161 ggttctggat ggatagaaga tcaggacgca cccatccaac caatccaaga attccctgtt
     5221 gacatcacga gctaccaaac cgttatagaa gacaaagaaa acattacatt gaccctaaaa
     5281 aaacaagttt tcttacctta tccttatgga aatactacct tgactgattt ttcattagct
     5341 agtggacaaa cgctttacta tttgcctgaa agccagcgat tcattcctgt cccgaatgaa
     5401 tcgacacgtt ctgcaacttt acaaacagaa aggttagttt ttacgagaga agaattaaaa
     5461 agcagtcagt taaaagctga tgatttagaa aatcagcaga caaatcttca attgccaaaa
     5521 agtttgccta aacgtattga ggacttggcg gtcgaattga ccaaaaatga agcaacttta
     5581 attcgtaaag tcgaagctgt ctaacagtat ttaaaaacaa gcggaaattt ccgctattcc
     5641 aaaacggata ccgggcatac tccaaaagaa cacgattatg tggattattt cctctttgat
     5701 tcaaaagtcg gctattgtga caacttttca acttctatgg tcgtccttct tcgaacactt
     5761 ggtatcccag ctcgctggac aaaaggtttt acaaatgggc tcagaacagg cacagataat
     5821 caagggaatg aggaatacag cattctcaat agtcatgctc attcttggcc agaggtatac
     5881 tttcctggat ttggctggct tccatttgaa ccaacccctt catttgccaa tccaatgggt
     5941 tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa
     6001 agcgaaaata tggcagaaac tatttccagt gagagatctg aggtcattcc atcagaagaa
     6061 acaacactca caccagctta taaaatcgtc ttattcagcc ttggagtgtg tttatccgtc
     6121 ttgttgttct ttctattgtt attcagaaaa tctctgcgct ggacaagtta tcttatctat
     6181 cttcattttt ttgcttctac taatacgatt gcatatcaaa aacttcttaa ccctttggaa
     6241 acattggtac cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg aatagaacaa
     6301 gaattcaagc tagatctgcg tttcattcga ttaactactc aatatgaagc agaaatatat
     6361 ggacgacaag cagatccggt aaaattcgat aaaaaaacct atttagaaat aacgcatata
     6421 ataagaaaaa tggtatacaa aaaaattctg acaaaaggga gccgcttcaa aaaataagat
     6481 aaaatttctg gagaatctac ttctggaatg ccgagaacca gctgattgag tatcgattac
     6541 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
     6601 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
     6661 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
     6721 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
     6781 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
     6841 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
     6901 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
     6961 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
     7021 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
     7081 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
     7141 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
     7201 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
     7261 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
     7321 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
     7381 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
     7441 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
     7501 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     7561 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     7621 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     7681 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     7741 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     7801 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     7861 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     7921 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     7981 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     8041 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     8101 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     8161 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     8221 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     8281 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     8341 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     8401 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     8461 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     8521 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     8581 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     8641 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     8701 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     8761 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     8821 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     8881 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     8941 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     9001 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     9061 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     9121 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     9181 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     9241 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     9301 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     9361 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     9421 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     9481 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     9541 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     9601 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     9661 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     9721 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     9781 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     9841 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     9901 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     9961 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
    10021 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
    10081 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
    10141 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
    10201 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
    10261 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
    10321 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
    10381 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
    10441 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
    10501 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
    10561 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
    10621 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
    10681 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
    10741 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
    10801 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
    10861 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
    10921 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
    10981 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
    11041 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
    11101 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
    11161 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
    11221 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
    11281 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
    11341 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
    11401 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
    11461 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
    11521 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
    11581 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
    11641 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
    11701 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
    11761 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
    11821 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
    11881 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
    11941 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
    12001 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
    12061 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
    12121 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
    12181 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
    12241 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
    12301 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aaggctctat
    12361 aatttatggg actgatgaat cagaattgat tcttcagccc catttttatt ttaggtatta
    12421 aaaaacatat cgttctctag tatgattaaa tcacgacaaa ataaacaact gagaggacga
    12481 tatgctatat gaatgattct atcaaaaaaa tgctgcgatt aa
//
LOCUS       DARDHD010000077        12233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000077 DARDHD010000000
VERSION     DARDHD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.077"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..751
                     /locus_tag="SDA06_001630"
     CDS             68..751
                     /locus_tag="SDA06_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG4228905.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="SDA06_001631"
     CDS             753..1589
                     /locus_tag="SDA06_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4228906.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="SDA06_001632"
     CDS             1600..3354
                     /locus_tag="SDA06_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228907.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="SDA06_001633"
     CDS             complement(3630..3971)
                     /locus_tag="SDA06_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4228908.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="SDA06_001634"
     CDS             complement(4039..6210)
                     /locus_tag="SDA06_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4228909.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="SDA06_001635"
     CDS             6379..7278
                     /locus_tag="SDA06_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4228910.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="SDA06_001636"
     CDS             complement(7349..8206)
                     /locus_tag="SDA06_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4228911.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="SDA06_001637"
     CDS             complement(8191..10893)
                     /locus_tag="SDA06_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4228912.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="SDA06_001638"
     CDS             complement(10906..12195)
                     /locus_tag="SDA06_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG4228913.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         3381 a         2604 c         2221 g         4027 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa aga
//
LOCUS       DARDHD010000078        12016 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000078 DARDHD010000000
VERSION     DARDHD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.078"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(119..706)
                     /locus_tag="SDA06_001639"
     CDS             complement(119..706)
                     /locus_tag="SDA06_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228914.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            881..2203
                     /locus_tag="SDA06_001640"
     CDS             881..2203
                     /locus_tag="SDA06_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4228915.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2358..3134
                     /locus_tag="SDA06_001641"
     CDS             2358..3134
                     /locus_tag="SDA06_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4228916.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3127..3696
                     /gene="rnmV"
                     /locus_tag="SDA06_001642"
     CDS             3127..3696
                     /gene="rnmV"
                     /locus_tag="SDA06_001642"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG4228917.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3689..4573
                     /gene="rsmA"
                     /locus_tag="SDA06_001643"
     CDS             3689..4573
                     /gene="rsmA"
                     /locus_tag="SDA06_001643"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG4228918.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5024..5653
                     /locus_tag="SDA06_001644"
     CDS             5024..5653
                     /locus_tag="SDA06_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228919.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5777..6886)
                     /gene="ugpC"
                     /locus_tag="SDA06_001645"
     CDS             complement(5777..6886)
                     /gene="ugpC"
                     /locus_tag="SDA06_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG4228920.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7095..7517
                     /gene="mgsA"
                     /locus_tag="SDA06_001646"
     CDS             7095..7517
                     /gene="mgsA"
                     /locus_tag="SDA06_001646"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG4228921.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7840..8376
                     /locus_tag="SDA06_001647"
     CDS             7840..8376
                     /locus_tag="SDA06_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4228922.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8416..10179)
                     /locus_tag="SDA06_001648"
     CDS             complement(8416..10179)
                     /locus_tag="SDA06_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228923.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10182..11897)
                     /locus_tag="SDA06_001649"
     CDS             complement(10182..11897)
                     /locus_tag="SDA06_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228924.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3741 a         2222 c         2453 g         3600 t
ORIGIN      
        1 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
       61 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
      121 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
      181 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
      241 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
      301 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
      361 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
      421 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
      481 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
      541 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
      601 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
      661 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
      721 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
      781 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
      841 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
      901 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
      961 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
     1021 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
     1081 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
     1141 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
     1201 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
     1261 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
     1321 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
     1381 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
     1441 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
     1501 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
     1561 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
     1621 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
     1681 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
     1741 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
     1801 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
     1861 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
     1921 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
     1981 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
     2041 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
     2101 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
     2161 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
     2221 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
     2281 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
     2341 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
     2401 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
     2461 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
     2521 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
     2581 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
     2641 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
     2701 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
     2761 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
     2821 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
     2881 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
     2941 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
     3001 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
     3061 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
     3121 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
     3181 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
     3241 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
     3301 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
     3361 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
     3421 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
     3481 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
     3541 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
     3601 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
     3661 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
     3721 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
     3781 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
     3841 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
     3901 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
     3961 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
     4021 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
     4081 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
     4141 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
     4201 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
     4261 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
     4321 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
     4381 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
     4441 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
     4501 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
     4561 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
     4621 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttgttac
     4681 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
     4741 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
     4801 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
     4861 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
     4921 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
     4981 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
     5041 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
     5101 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
     5161 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
     5221 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
     5281 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
     5341 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
     5401 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
     5461 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
     5521 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
     5581 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
     5641 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
     5701 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
     5761 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
     5821 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
     5881 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
     5941 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
     6001 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
     6061 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
     6121 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
     6181 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
     6241 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
     6301 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
     6361 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
     6421 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
     6481 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
     6541 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
     6601 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
     6661 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
     6721 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
     6781 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
     6841 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
     6901 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
     6961 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
     7021 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
     7081 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
     7141 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
     7201 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
     7261 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
     7321 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
     7381 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
     7441 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
     7501 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
     7561 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
     7621 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
     7681 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
     7741 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
     7801 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
     7861 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
     7921 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
     7981 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
     8041 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
     8101 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
     8161 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
     8221 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
     8281 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
     8341 aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca
     8401 gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc
     8461 atctttttga agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac
     8521 taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt
     8581 tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga
     8641 gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt
     8701 caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa
     8761 tttttccggc aaacgttcta caaattcata catataagag gcttttaggg catcattgat
     8821 ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa
     8881 tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga
     8941 gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag
     9001 caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc
     9061 tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga
     9121 aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg
     9181 caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa
     9241 agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact
     9301 tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc
     9361 caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga
     9421 cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt
     9481 tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg
     9541 gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc
     9601 gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga
     9661 acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat
     9721 gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt
     9781 gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg
     9841 aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta
     9901 agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat
     9961 accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat
    10021 catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat
    10081 gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa
    10141 tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat
    10201 gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca
    10261 gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt
    10321 tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag
    10381 tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca
    10441 atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt
    10501 tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg
    10561 attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt
    10621 tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg
    10681 agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg
    10741 acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca
    10801 atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca
    10861 gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa
    10921 tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc
    10981 acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc
    11041 agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag
    11101 ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg
    11161 atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc
    11221 gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg
    11281 cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt
    11341 tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag
    11401 aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg
    11461 accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa
    11521 gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa
    11581 tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct
    11641 gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga
    11701 ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc
    11761 atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca
    11821 gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa
    11881 cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa
    11941 gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa
    12001 tgaaagtttt attatg
//
LOCUS       DARDHD010000079        11927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000079 DARDHD010000000
VERSION     DARDHD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.079"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SDA06_001650"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SDA06_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG4228925.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SDA06_001651"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SDA06_001651"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG4228926.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="SDA06_001652"
     CDS             complement(2169..2906)
                     /locus_tag="SDA06_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4228927.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="SDA06_001653"
     CDS             complement(2997..3524)
                     /locus_tag="SDA06_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228928.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="SDA06_001654"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="SDA06_001654"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG4228929.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="SDA06_001655"
     CDS             complement(4401..4886)
                     /locus_tag="SDA06_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG4228930.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="SDA06_001656"
     CDS             complement(5039..5236)
                     /locus_tag="SDA06_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228931.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SDA06_001657"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SDA06_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG4228932.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SDA06_001658"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SDA06_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG4228933.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="SDA06_001659"
     CDS             9211..9423
                     /locus_tag="SDA06_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG4228934.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="SDA06_001660"
     CDS             9425..11104
                     /locus_tag="SDA06_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4228935.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="SDA06_001661"
     CDS             complement(11358..11558)
                     /locus_tag="SDA06_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4228936.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="SDA06_001662"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="SDA06_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3455 a         2573 c         2072 g         3827 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtg
//
LOCUS       DARDHD010000080        11876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000080 DARDHD010000000
VERSION     DARDHD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.080"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            240..1358
                     /locus_tag="SDA06_001663"
     CDS             240..1358
                     /locus_tag="SDA06_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228937.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1861..2181
                     /locus_tag="SDA06_001664"
     CDS             1861..2181
                     /locus_tag="SDA06_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228938.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            2634..2834
                     /locus_tag="SDA06_001665"
     CDS             2634..2834
                     /locus_tag="SDA06_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4228939.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            2981..5770
                     /locus_tag="SDA06_001666"
     CDS             2981..5770
                     /locus_tag="SDA06_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4228940.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            5817..6344
                     /locus_tag="SDA06_001667"
     CDS             5817..6344
                     /locus_tag="SDA06_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG4228941.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            6365..7555
                     /locus_tag="SDA06_001668"
     CDS             6365..7555
                     /locus_tag="SDA06_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4228942.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            7595..8893
                     /gene="asnS"
                     /locus_tag="SDA06_001669"
     CDS             7595..8893
                     /gene="asnS"
                     /locus_tag="SDA06_001669"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG4228943.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            10069..10743
                     /locus_tag="SDA06_001670"
     CDS             10069..10743
                     /locus_tag="SDA06_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4228944.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            10740..11081
                     /locus_tag="SDA06_001671"
     CDS             10740..11081
                     /locus_tag="SDA06_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228945.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            11111..11470
                     /locus_tag="SDA06_001672"
     CDS             11111..11470
                     /locus_tag="SDA06_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG4228946.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3969 a         2086 c         2542 g         3279 t
ORIGIN      
        1 aatgcataag atccacctct tttattcttt ttcttagttt atcagcattt catgaagaat
       61 agttgaagac tggggctaaa taattgagga aattatgaag ataacttaaa aaaatagcta
      121 aatttaaaac cgtagaaacc gttttatttt tttagatacg ttttattgta tggaaaaata
      181 gtgttgtggt agactttaga tagaaacaaa ggaatgattt ttttaggagg ggtacgttta
      241 tggcaaaaaa ggataaagaa ttattgtcac aattgacttc agaaggcgct aaaggaccat
      301 tcgtcacgat catgctgaat acgcatgtcg cccatcaaga tgtggaaaaa gatcagatca
      361 aattcaaaaa ttttgcgaaa gaagcaaaga aacgttttga gaaaaaatat tccgaacatg
      421 actggtcccc gttccaagaa aaaattgaaa atttgttagg agatccttcg ttttggcgta
      481 gtgcaactac gagtgtagcg gtcattataa ctgctgacca aacctatatc catcgtttga
      541 gtatcccggt agacgatcaa tactatgtaa gtgatcttcc ttatttactg gctattatca
      601 aaaatgggca gttcaactat tcttattatt tattgggatt gaaccgcgac tccatgaaat
      661 tgtatgaagt cagcaacaaa caagtaaaag aagtatctct accagaaggg gcaccggttg
      721 atgtcgttac tgcattggga tctgaactga caggtggcgg tttgaacttc tcttctaatg
      781 gaggaggcgg agcccgagat ggagtaactt tccatggcgt aaatccaaaa gatgaagaag
      841 tagagatcga ttgggtcaac tattaccaag cagttgatac gtacttgaag gattctttcg
      901 aaaatccaga acaatggccg atttatttat ttgctctacc agaaaatcag acgatgttca
      961 aaaaaattgc taaaaatccc tattattgtg cagacgttgc gatttctgct tctcctgcgc
     1021 agctatcctt tagagatatc gaagaagggg cgaagaagct gtcaagtgaa ctcgcagctc
     1081 aagaatcaaa agaatacaat aaactcttag ataagaaatt catcgatcag ttagttgata
     1141 tcgttccagc tgcgaaagaa ggaaaagtgt cacatttatt tatcgctacg tcaaacttag
     1201 ctgatggttt cggtgaagat cctgaaacag aatatgatcg gcgtcaagtg ttgaatgcat
     1261 tagcagatga tgtgatcatt aatggcggaa aagtgtttat tttggatcaa aaagatgcgc
     1321 cagatgaaaa gagtctgaca gcgattcttc gttattaaaa gaggttgtga aatcagtgtt
     1381 ttgacctgag tattagagaa acaaacggaa aaatagtttc acatattttg ccgttttttg
     1441 agctaatacc gaaggtcagc tgattaaaca ccgtgtatga gaggttgtga aaaagctgtt
     1501 ctgcatcaag taataagaac agcaagcaaa aatagctctt catatttttt gtttggtggg
     1561 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gacagaagtg
     1621 gtcagcttca agaaataaga agagattgac aaaaattatt tctcaaattt ttgtgaaatt
     1681 tcggcttatt tccgaagaag ctacttctgg aacaccgttt aaaaaagagg ttgtgacgtg
     1741 gtcacgacct ctttttgacg attatgatac aaaaaacatc atttatgaac aaaatgtgaa
     1801 taacgggcgt tcttgagata tgattttaat tgggaaaaac ttatcgataa ggaggtcttt
     1861 atgaaaaaaa gactgttatg gtgtttgctg tctctatccc taagtttaac aagtcagcca
     1921 gtttttgctg aagaaacaca gcggtttcct attacaggca cacgtctgta caaaaatgtt
     1981 tatgtacaga caccagatct tgattctgcg attcctgaaa cagaagaacg gtctttgcag
     2041 ccaagatttg gttacttttg gtactgtccg acctgtgatt atgaaagttg ctggcatttc
     2101 ttacattcta cagctgcagc ttttgctttt actcatactc agaaatatga tggacatcat
     2161 cctgttattt atgaggtata gatatgagca aaaaagaaaa actaatctcg ttggtcgtat
     2221 tggtttgcct tgttcttctg gtaactgtca taggctatca gaaataccat gagaacatca
     2281 gacctactaa agaatcttcc ccagcctcga ccgttgaaac agaaccgata agtagtcaga
     2341 aagaataatt tttttagttt aaaaaagaac atcagagcaa aaaaacacca actgaacaat
     2401 atgtcgttta ttttaaaaaa aagttcggtt tttcacttta tgtaatcgct gagaaaactt
     2461 tttttaataa aaaaagtcga aaaactctag cttttttgta aacggtatga tatactatag
     2521 gagtagtttt tgttcggtaa tctgtggtag cttgtttcaa ataaacatct tccagatata
     2581 caccattcaa gtaattgcaa aatatgtgtt gatacacaag gaggattttt attatggaaa
     2641 caggtacagt aaaatggttt aactcagaaa aaggatttgg attcatcact gcagaaaacg
     2701 gaaacgatgt attcgttcat ttctcagcta tccaaggcga cggattcaaa actttagaag
     2761 aaggccaagc agtaactttc gatgttgaag aaggccaacg cggacctcaa gcaacaaacg
     2821 ttactaaagc ttaattaacc tatactaaaa aataaacgcc cagacatttg cgtgtctggg
     2881 cgtttttgtc tattttcttt aaaaagcgat gagagtattt ttctgcactt tgtagtataa
     2941 tttcaaaaga acattagagg caggagaaga ggaagatatt ttgaaaccaa gaacttatgc
     3001 agtcgttgat atcgaaacga ctgggacgaa tccaaaagaa gatcggatca tccaatttgg
     3061 ctgcgtcatg attgaatcag gtcggatcac ttcacgtttt tcgatcgata ttcatccagg
     3121 gcggaagatt tcaaaacaaa tccagcattt aacagggatc acgaatcaac gcgtccaaaa
     3181 agcgccttat ttcgaagatg tcgcccaaac gatttacaat cttttggcag acaccatatt
     3241 tgtcgcgcat aatatttatt ttgattataa ttttttgaac catgaactca tgcgatgcgg
     3301 acttcctagt ttgaagatac caggcatcga tacagtagaa cttgcacaaa ttttcttgcc
     3361 gacggaacca agctttcgct tagctgattt gtcagaaagc cttggattta ttcatgagaa
     3421 tcctcatcag gcagatagtg atgccgaagt gacaggtcaa cttcttttgc tgatcgaaga
     3481 acggatgaga aaattgccaa tcatcacgtt agagcagatt gcgcgattaa gtagacacac
     3541 aggtatggat acgagtcgtt tcatttatca tgtgattgaa gaaatgaaag aaaaaccaga
     3601 gccacttgat ccctcattag aaatcgtcga tgggctagct ttgcagaaaa aagaagtgga
     3661 actattcaca tccgtccatt atggcgagcg gacatatcca agaaaaaaac aagcaaaaga
     3721 aaaaatgttt ggtaaaacac tgatgtatcg aaaagaacag aacagactga tgaatgcggt
     3781 ctacgaccat ttcacaaaag atgaatcaaa agatttgatg atcgaagcag ctacaggaat
     3841 gggtaaaaca atcggttatc tgctgccatt gagttatttg gctacacctg aaaaacccgc
     3901 tgtcatcagt accgtttcgc tagttttgca gcagcagatc atagaaaaag acattccatt
     3961 gctgaatcaa ttgctagatc agcccataca acctgtaatt atcaaaagct atcgccacta
     4021 tattgatttg cagcgtttta aaggcacact tgtggaaccg ccagaacaaa aacaatatgc
     4081 tttgtatcaa atggcggtct tagtttggct gacgcaaacg aagactggtg atttggatga
     4141 attacattta acaaatttga accattcatt ctttgcagat atcgcgcaca gaggtattgg
     4201 atttttagca agaaatcagt cattttacga acaagacttt gttcgtttct tgcaaaagaa
     4261 aatcaggcaa agtaattttt tgattgtcaa tcatgcgttt ttagtacaag aaacacagcg
     4321 aaaagaacca ttgcttccag ctagtccatt tttgttgatc gatgaagtac atcatttgcc
     4381 ggaaatcgct gaaaaagtaa atggcaaacg attaagtatt aactatttcc ataaacaaat
     4441 ccagcaatta gaagaaccaa atcagctgtt tgaccgaata caaagctatt taccgaaaga
     4501 acatgaagcc ttacgctggt tatcgattta tcgagaagaa ctttctgcgc tatgcgaagc
     4561 acagatttgt atggcagaag gattaatgag tttagtgagc ggcaagcaac ctaacggaca
     4621 gtatccagaa gaagtcatga tgacaaaaga gatgttggat cagctgccac tggaatctgg
     4681 gacagccatt gatcacctgc agctttttta tcaggagatc cagcagctac agtcacaaat
     4741 gcgtctgact tttgagatta tccaagaaag ctggacaaac cagatgcgga tcgatttagc
     4801 aagtttatat agcctatttg atcaattaga caagcaatac ttgctgatgg atcaatggct
     4861 ggaagactgg cgagatcatt tgatccattg gttcgttcct gggaacacag cgcagcagat
     4921 gatttttcat ctccatgatt ttcaagcggc tatgttgccg tcgactgttt ggtatcctcg
     4981 atatgatcgt attctttata ttggaggaac gctgaagatc ggccctaatc gacactatct
     5041 ctctaagagg cttggcattg aagcagcacc tcttaaagtg attgcctctc cttatgatta
     5101 tgagaaacag gcaaggatct atgtgccaaa agaggcacct gccgtccatc aagtcagcaa
     5161 tcaagcatat atcgattatt tagagacagc acttacaggt ctgatcgaac aagaagatcg
     5221 gccaatactt gtgttattca cttctcatga tgttttgcag aaggtctatc agaaaatgca
     5281 tctgtctatg ctagaaaaag gacgagaaat actagctcaa gggtttggtg gaagccggga
     5341 gaagcttttg aaacgcttca ttttgtcaga aaaaagcgta ttgtttggcg ctgacagctt
     5401 ttgggaaggt gtagatttgc ctggagatgc tttgcagatc gttgtagtca cacgtttacc
     5461 cttcgataat ccgcttcgcc cgatggtcaa agctaggaac gcttatttag aatcagaggg
     5521 gatcaatcct ttttaccagg aatccgtacc taaagcagct ttgaagttac gccaagcatt
     5581 aggtcgcttg ataaggacgg agacagatcg tggcgtattg cttattttgg atcgaagact
     5641 agtcactgca aaatatggcg cacggatcat ccatgcatta ccgaaaaaag taccgatcaa
     5701 agagctagct atggaggaaa tctatcagga tatccatgaa ttcttaaaga aaaatgaaga
     5761 aggaagttag aggagctgtc ttttttcata attaaaatct gctataatga aaaggaatga
     5821 agataaagga gcgacgaccc ttgaagaaaa aacagacaaa ggaaaaaacg attaatcgcg
     5881 tgttacttgg cttgatcgta tttttattag cggtcatcgt gatcagctcg attttttact
     5941 tgcgttccag tcgtccggcg gtgcaggcga agaaagaagc agtagaaatc gcaaagaaat
     6001 atgcaaatct tgaggaaacc gatcattttt attggtttac tcgaaagaag acatactttt
     6061 cactgacagg tacaaatgat aaaggaaaag aaatcgccgt tatcattcct aaatctggtg
     6121 aaaaagtaaa agtattggat caaacaaagg gactgactga aaacgaagca aaacagacaa
     6181 ttgctgcggc ccatcctgaa atccaaatcg aaaaagctgc tttagggatg tatgatgatc
     6241 agcctgtgtg ggaagtagta ggaaaagaaa caaatgggaa actgaattat tacctgattg
     6301 gctttgaatc tggagatgaa gtcaagacaa tcaagggaat ttaaaaggta aaggaatgaa
     6361 tgggatggag ttatcaaaaa gaacaaaacg attagaaccg tctgtcactt tggcagctgc
     6421 ggcaaaggcc aaggcattaa aagcaaaagg cagagatgtg ctgagtttga ctgtgggaga
     6481 accggatttt gcgacaccag aaaacatcca agaagcagca atcgaagcaa tccgcaatgg
     6541 gaaagctagc tattatacac caacagcagg aatcccagag cttcgacagg caatcgttga
     6601 ttatctgaag aaaaactatg gacttgtcta tgaaccttca caaacagtcg taacagacgg
     6661 agcgaaattt gctttgtata cccttttcca aacgatcctt gacccgcaag atgaagtcat
     6721 cattcctgtg ccttactggg tcagttacgg tgagcaagta aaactggcag aaggacttcc
     6781 tgtctttgtg aaaggggaag aatccaatag ctggaaagtt actgttgaac agctagaaca
     6841 agcacgttca gaaaaaacaa aagccgtaat catcaattct ccatcgaatc cgactggaat
     6901 gatttatagc aagaatgaac tgcaggcaat cggtgaatgg gcagtaaaaa atgatatttt
     6961 gatagttgct gatgatattt atggccgttt agtttataat ggcaatgagt ttactccaat
     7021 tgcaacgatt tcagaagcaa tcaaaaatca gaccatcatc atcaacggcg tgtctaaaac
     7081 atatgcgatg acaggctggc gcattggtta tgcggtggga aatcctgtga tcatcaatgg
     7141 aatgatcgct gttgcttccc aatcaactag caatccgaca gcagtcagtc aatatgcagc
     7201 tgttgaagca ttgaaaggtg agcaagatac agtagaagaa atgcggaacg cctttgaaga
     7261 acgcctgaat acgttatatc cgcttgtggc agaactgccg ggtgtttctc ttgaaaagcc
     7321 gcaaggagct ttctatcttt ttcctaatgt aaaagaaacg ctacgcatgt gtaagtatga
     7381 aaatgtcaca caatgggtag aagatctttt agaagaaaca ggggtagcgc ttgtgactgg
     7441 cgaaggcttt ggggcaccag aaaatgtccg catgagttat gccactgatc gcgctacgtt
     7501 agaagaagcg gtacgcagaa tcaagcaatt tattgagagt aaaagtcaaa attaatcctg
     7561 aagaaattca aggaatataa aaggagagtc atacgtggaa caaattcaaa tcatcgattc
     7621 gaaaaaccat gtgggagaaa cagtaacaat cggcgcttgg gtcgcaaata agcgatcaag
     7681 cggaaaaatc gcatttttac aattaagaga cggaacagcc tttttccaag gtgtagttgt
     7741 aaaaagcgaa gtgtcggaag aagttttcca aacagctaaa aatctacacc aagaaacatc
     7801 cgtgttagta actggagaaa tccgtgaaga cagtcgttca aaatttggtt atgaaatcgg
     7861 aattacaaat gtggaagtgg taagcgagag ccacgattat ccgatcacac caaaagaaca
     7921 cggaacagac ttcttgatgg atcatcgtca tttatggctg cgttcttcaa gacaacatgc
     7981 gattatgcag atcagaaatg aaatcattcg tgcaacgtat gaattcttta atcaacatca
     8041 ttttgtcaaa attgatccgc cgatcttgac tggttctgca ccagaaggca ctacggatct
     8101 attcgaaaca aattactttg atcaaaaagc ttatttatct caaagtggcc aattatacat
     8161 ggaagctgct gctatggctt tcggaaaagt cttttcattc ggtccgactt tccgtgctga
     8221 aaaatcaaaa acacgacgtc atttgatcga attctggatg atcgaaccag aaatggcttt
     8281 cactcatcaa gaagaaagtt tagaagtaca ggaacaatat gtagcattct tagtacaaaa
     8341 tgttttagat cattgcgaat atgcattaga tgtactagga cgagacaaag aagtcttaaa
     8401 aacttataca aaattgccat tccctcgtat tacttacgat gatgcaatcg aattactgaa
     8461 gaaaaatgga tttgatgaca tcgaatgggg cgaagacttt ggttcacctc atgaaacgtt
     8521 cattgcgaat tcattcgaac agcctgtctt tatcctaaac tatccaaaag ccatcaagcc
     8581 attctacatg aaaccacatc caacaagaga agacgtggta atttgtgcag atatgatcgc
     8641 accagaaggt tacggtgaaa ttattggcgg aagcgaacgg gcaacagact atgatttctt
     8701 gctagaacaa atccgtaatc acggtttgga tgaaaaagaa tattcatggt atcttgatct
     8761 acgtaaatac ggttctgttc cgcactctgg ttttggtcta ggccttgaac gaacagtcac
     8821 ttggattgct ggtatcgacc atgtacgtga agctattcca ttcccacgtt tgttgaaccg
     8881 aatttatcca taagaggtga tgaaaaagct gttctgcatc aagtaattag aacagccaag
     8941 caaaaacagc tgttcatgtt tttgaattgg cgggacttat taccgcagat gtaagctttt
     9001 gaataccgtt tagtggagag gttgtgaaaa agctgtttag tttcgagtat taagaaaaat
     9061 tttgtggaaa tggctttcca catttttgca taaatttggc ttaataccga gaaactggct
     9121 tttgaacacc gtttaaggaa gaggttgtga aatcagtgcc ttgacctgag tattagagaa
     9181 acaaaacgga aaaatagctt ctcatatttt accgtttttt tagctaatat cgagggtcag
     9241 ctgattgaac accgtttata tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa
     9301 ggagtaattt caaaaaacag tttttcatgt ttgatgacta ttgtgacttg tgaccgagga
     9361 gttggccgga gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt
     9421 ttaaaatacg ggataaagga agtttccagc agacaagaga agaaagcaaa agaaattgct
     9481 atttgattca caaggttcta taacgaaatc gcaatcaatc aaacatatac aaagaaggtt
     9541 attcaaatgg aaatctgatt tgatgatgct tctgctgttg aaataaagca gcatctagta
     9601 cctgaaaaca aactaatagt aacattcgaa gatggagtag gtccgtattt tcagcatgca
     9661 atgatccata tgtaagttta attcatcctt gaaatcatcg gaccagataa agttatgacc
     9721 aatctatccc aagcaatcta gaagagatat tagtaaaaga acatttactg gaagtcttag
     9781 atgagcctat aaatatatgt tctgatggcg tcatcagtgg tctgattgac ggaagcttag
     9841 gtgtgatcga tttctctgga ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag
     9901 aaaaaaagta taaaaaacta gacacttgat gagcgttcat tcaattgatt gaagggacta
     9961 ggctggtgtc attttttata catatatttt ctctttcgtc ttgatagggc ttatgggata
    10021 atataaggaa gaaaatcatc gttttattca aaaaggagtt tttgacaaat gaaaaaacgt
    10081 atattattta tcggtgacag cattacagac gcaggaagag accgagcaga agcaactgat
    10141 ctaggtaaag gttatccact tttgattgcc ggaaaactaa tctcccaata tccaacgatg
    10201 aaatttgaat gcatgaaccg tgggatagga ggagacaaaa ttgcagatct gcaagtacgc
    10261 tggcaagaag attgtttagc tttacaacct gactatgtat cgattttgat cggaatcaat
    10321 gatacctggc ataatactag cgatcaggct atattcggaa ctgagaaatc tgcgcagcaa
    10381 tttgaatccg tttatcgaaa attactaaat gaacttcaaa cagcggaaat ctgcaacatc
    10441 atcttgatgg agccgtttgt acttcctata accgacgacc gtcatggctg gcgtaaagac
    10501 ttagacccta aaatccaaat tgttcgtaaa cttgctaagg aatatggcgc attgctggtt
    10561 cctttagatg gcatattcaa tgctttaggg attgcttatg gtttttcgaa atatacaaga
    10621 gaagatgggg tccacccaac gattggcgga catgagatca ttgcacaaag ctggatagat
    10681 gcaattgccg cagtcgattt ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa
    10741 tgatggaaat acggtgggca gtaaaagaag atttacctca aatcaatcag ctgattactg
    10801 atcatccaga atcattcgta aaagcaaaaa cggcagctga cctaaatgat aagaataaac
    10861 tattgctcgt tgctgtaaca aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc
    10921 aacagtcaga agaacgggtg gatcagcgac catcaggaaa actaatccaa cacacattag
    10981 taacaaaaga aaatacggaa gaaatccaaa aaaaatttgc agaatacttg atgctggaag
    11041 ctaaaaaaag aagaataaaa tcaatttttg cccagtttta atcaaaaaca gtaatagaag
    11101 gagaaataca atgagttgtt taggaaatgt catctggttt atattcggcg gactaatcgg
    11161 tggtcttagc tggttagcag cggggatact ttggtgtatc acgatcatag gtatcccaat
    11221 cggtatgcaa tgttttaaga tcgcctccct ttcttttgcg ccttttggca aagaagtaat
    11281 ttatcaccag tctggcatgt cattgatcgt taatatcatc tggctgatcg tttcaggcct
    11341 accaattgct ctcgcacatg tcgtcagcgg catccttcta tgtatcacga tcatagggat
    11401 accttttgcc aagcaatctt tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga
    11461 aattgtataa aaagagaata ataaaggtta gaaaacgcat agagtcaggt gggatacatg
    11521 tatttcacct gactctatgt gttttcctgt ataagttctc ccatgtgact tttacgtctt
    11581 ttatttatat gggattactg tttatggaaa gttgaaataa ttttcattaa agaactcttt
    11641 ctatattttt gaaagcgtag tggagcttcg aatatctggg aaggacattt tgcagggatt
    11701 gaaaaaagat attgtcctat cgaaaaatta atgaatttag acaaattgcc agcaacaggc
    11761 ttcaaactta gttgctatcc tgtcaagatc aagaaggcaa gtgcgggttg gataagagca
    11821 ggggcaatga tcgaagagaa aaagaaagaa taagccaaaa catctgtatg gaagaa
//
LOCUS       DARDHD010000081        11693 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000081 DARDHD010000000
VERSION     DARDHD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.081"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SDA06_001673"
     CDS             complement(37..216)
                     /locus_tag="SDA06_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228947.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SDA06_001674"
     CDS             complement(597..1304)
                     /locus_tag="SDA06_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228948.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SDA06_001675"
     CDS             complement(1297..1734)
                     /locus_tag="SDA06_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228949.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SDA06_001676"
     CDS             complement(1749..2000)
                     /locus_tag="SDA06_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228950.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SDA06_001677"
     CDS             complement(2000..2206)
                     /locus_tag="SDA06_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG4228951.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SDA06_001678"
     CDS             complement(2643..2903)
                     /locus_tag="SDA06_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228952.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SDA06_001679"
     CDS             2978..3181
                     /locus_tag="SDA06_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG4228953.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDA06_001680"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDA06_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4228954.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SDA06_001681"
     CDS             complement(3810..4709)
                     /locus_tag="SDA06_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG4228955.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SDA06_001682"
     CDS             complement(4722..5315)
                     /locus_tag="SDA06_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG4228956.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SDA06_001683"
     CDS             complement(5391..5540)
                     /locus_tag="SDA06_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228957.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SDA06_001684"
     CDS             complement(5556..7760)
                     /locus_tag="SDA06_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG4228958.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SDA06_001685"
     CDS             complement(7771..9231)
                     /locus_tag="SDA06_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG4228959.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SDA06_001686"
     CDS             complement(9292..11664)
                     /locus_tag="SDA06_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228960.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2898 a         2439 c         1959 g         4397 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca ttt
//
LOCUS       DARDHD010000082        11558 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000082 DARDHD010000000
VERSION     DARDHD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.082"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SDA06_001687"
     CDS             complement(105..629)
                     /locus_tag="SDA06_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228961.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDA06_001688"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDA06_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG4228962.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDA06_001689"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDA06_001689"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG4228963.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SDA06_001690"
     CDS             complement(2351..3031)
                     /locus_tag="SDA06_001690"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4228964.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SDA06_001691"
     CDS             3157..4026
                     /locus_tag="SDA06_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4228965.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SDA06_001692"
     CDS             4052..4567
                     /locus_tag="SDA06_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228966.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SDA06_001693"
     CDS             complement(4614..5207)
                     /locus_tag="SDA06_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4228967.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SDA06_001694"
     CDS             complement(5232..5411)
                     /locus_tag="SDA06_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228968.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SDA06_001695"
     CDS             complement(5488..5997)
                     /locus_tag="SDA06_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228969.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SDA06_001696"
     CDS             complement(6093..6473)
                     /locus_tag="SDA06_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4228970.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SDA06_001697"
     CDS             6803..7552
                     /locus_tag="SDA06_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG4228971.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SDA06_001698"
     CDS             complement(7736..9013)
                     /locus_tag="SDA06_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228972.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SDA06_001699"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SDA06_001699"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG4228973.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SDA06_001700"
     CDS             complement(9935..10879)
                     /locus_tag="SDA06_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG4228974.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..>11558)
                     /locus_tag="SDA06_001701"
     CDS             complement(10881..>11558)
                     /locus_tag="SDA06_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG4228975.1"
                     /translation="YLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLE
                     LLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRM
                     IFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFG
                     QVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKK
                     YYPGKGQ"
BASE COUNT         3288 a         2454 c         2043 g         3773 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagata
//
LOCUS       DARDHD010000083        11491 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000083 DARDHD010000000
VERSION     DARDHD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.083"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..638)
                     /locus_tag="SDA06_001702"
     CDS             complement(93..638)
                     /locus_tag="SDA06_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4228976.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(650..1342)
                     /locus_tag="SDA06_001703"
     CDS             complement(650..1342)
                     /locus_tag="SDA06_001703"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG4228977.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1361..1645)
                     /locus_tag="SDA06_001704"
     CDS             complement(1361..1645)
                     /locus_tag="SDA06_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228978.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1658..2221)
                     /locus_tag="SDA06_001705"
     CDS             complement(1658..2221)
                     /locus_tag="SDA06_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4228979.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2449..3111
                     /locus_tag="SDA06_001706"
     CDS             2449..3111
                     /locus_tag="SDA06_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG4228980.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3235..4431
                     /locus_tag="SDA06_001707"
     CDS             3235..4431
                     /locus_tag="SDA06_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG4228981.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4532..5908)
                     /locus_tag="SDA06_001708"
     CDS             complement(4532..5908)
                     /locus_tag="SDA06_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4228982.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5945..6811)
                     /locus_tag="SDA06_001709"
     CDS             complement(5945..6811)
                     /locus_tag="SDA06_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG4228983.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6900..7925)
                     /locus_tag="SDA06_001710"
     CDS             complement(6900..7925)
                     /locus_tag="SDA06_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4228984.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7943..8617)
                     /locus_tag="SDA06_001711"
     CDS             complement(7943..8617)
                     /locus_tag="SDA06_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4228985.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8801..9694)
                     /locus_tag="SDA06_001712"
     CDS             complement(8801..9694)
                     /locus_tag="SDA06_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4228986.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9768..11465)
                     /locus_tag="SDA06_001713"
     CDS             complement(9768..11465)
                     /locus_tag="SDA06_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4228987.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3406 a         2522 c         1971 g         3592 t
ORIGIN      
        1 acgtataaaa aagagcataa aacaaagtcg tttgttttat gctcttcttt tattattttg
       61 tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc tagtgtggac
      121 tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac ggcatctttg
      181 aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa cacacagata
      241 tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa acggatgtct
      301 agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac attttcctct
      361 tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc acccgttcct
      421 atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt tggatcatgc
      481 gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt atgagcagtc
      541 agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc ctctgcaaca
      601 aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct tatacgagat
      661 gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg ataaattcgt
      721 caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg atcaaaaatg
      781 ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg caagctaatg
      841 cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca aatgtatcac
      901 ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct ttaaccattt
      961 cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat tgtcctggtt
     1021 tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat ataacgatgt
     1081 cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc accatgttca
     1141 cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt cctataccgg
     1201 aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa gcgcctgcgc
     1261 gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct tcttccattg
     1321 cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat gcgattgcta
     1381 aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa aaagagttcc
     1441 atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta ttctttttgc
     1501 tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct gtttctgctt
     1561 ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga agttcgcttc
     1621 gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg gtcaatagtt
     1681 cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct ttcatcgctt
     1741 gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca tcttgtgcta
     1801 aaggattctc gaccttctct accccttgtg catgataaat atgaaggact tcatttgcaa
     1861 aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga tagccagttt
     1921 cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct ggatcgattt
     1981 ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt ttgacaagaa
     2041 gcagacgatc ttgttcgtca aaagcaatga tcccgacacc accaggatga aataccagtt
     2101 ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca tcaataattt
     2161 ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca ttgttcagca
     2221 tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc aatttttctc
     2281 aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat atagaaaatc
     2341 cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat gtaatcgaat
     2401 aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat gataaaacga
     2461 aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa caatagtttt
     2521 tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg actctttggt
     2581 tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga acttgaaact
     2641 cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca aacaatgaat
     2701 caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc caaacttaaa
     2761 gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa agaattggtg
     2821 aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct gccgaatcta
     2881 gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa caaacagacc
     2941 tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt aatcgcacaa
     3001 gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat atctgttgcg
     3061 aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta actcttttgt
     3121 tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta ctttttctaa
     3181 agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt ttttatggat
     3241 cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga agatttattg
     3301 aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc ggagattgat
     3361 cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc ggaacgccaa
     3421 gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc tgtcatcagc
     3481 tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt gaatcatgtg
     3541 caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta tcgtctccaa
     3601 gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg tgtcttcggg
     3661 aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg tgcacaaatt
     3721 gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga taatctgatg
     3781 ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat ttatatcgct
     3841 gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc gatgaaaaga
     3901 gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac gcaattccta
     3961 gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac gatccagcaa
     4021 gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa gatccaagca
     4081 tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt gaccttgctt
     4141 cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa cgacttattg
     4201 aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga aaatgaacgt
     4261 ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga aacaatccaa
     4321 gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga agtagagctt
     4381 ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata gatcaccttt
     4441 cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat gggcgttttt
     4501 cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg ctgtgtccat
     4561 agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac acgatccatg
     4621 tcacagtttg cagacgaagc atcaatacaa tacaagtcaa gatggagacg attggcaata
     4681 gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt tttctcaact
     4741 tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc gttagttccg
     4801 ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca gcggcataag
     4861 tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta ggaagtaagc
     4921 catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag atcgttaccg
     4981 tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca tgtcctacat
     5041 acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga acgattcctg
     5101 tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa ccaatgattc
     5161 ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca acggcatcaa
     5221 atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa aaaccaaaag
     5281 gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg ataaacaacg
     5341 cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct ttcttggctt
     5401 caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag tcaatgtatg
     5461 taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc cctaaacttt
     5521 tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc gctagtaaaa
     5581 attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat acaacataca
     5641 gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg aaacttagtc
     5701 cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct ggaccagcat
     5761 tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc gcaccaattc
     5821 caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta tgattgagat
     5881 tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg ttggaaaaca
     5941 gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact aaaaggcaac
     6001 caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg aaaatcccac
     6061 ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct aaaccaagtg
     6121 atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca cagatcatgt
     6181 ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt gcgcctaaga
     6241 tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc cagttgacga
     6301 tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa cccttaccaa
     6361 aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg gcagtcaaat
     6421 acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct ttcgtttgtg
     6481 tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga aaaccagtag
     6541 acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt ccattttgtc
     6601 caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg gtgattacag
     6661 gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc attcctagtg
     6721 tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta ccccatgcca
     6781 acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt cacatgataa
     6841 gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct aacgattttc
     6901 taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt tgtccctttt
     6961 ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt ttttgctaaa
     7021 taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc agtaaatccc
     7081 ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc tttaactgaa
     7141 aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg atccggcgta
     7201 tatttgatgg cattactaag caattggttg aagataaaag cgatccattt agcatcggtc
     7261 aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat aaaataattt
     7321 gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg gatgaggtag
     7381 tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta ttcatcgatt
     7441 tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa ctcgatcgaa
     7501 cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca tgagtcaata
     7561 taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg gtgttcacgg
     7621 atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc accactcaaa
     7681 taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca tcttctcttg
     7741 ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa agctaaataa
     7801 gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa gatcgtcaat
     7861 gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata tttaaaaaaa
     7921 ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact tttgtttcga
     7981 tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg ttgactgtca
     8041 atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat ttttcccgac
     8101 ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca tttttgctca
     8161 aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac gtaatgccat
     8221 tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga agtaatgcat
     8281 tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca tctgccccca
     8341 tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa atgatcggta
     8401 ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg ggaagattaa
     8461 tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact tgattgaaat
     8521 cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc aattgtcgaa
     8581 tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc atctttcttc
     8641 ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg gaaaatgtca
     8701 ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg tctcttccaa
     8761 gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta tttttctttc
     8821 gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt agcactagca
     8881 atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc tgttctatct
     8941 ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat cggtgagtgt
     9001 tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct ctaattgcta
     9061 atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa gcgagcatcc
     9121 gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga tccgtaaaga
     9181 gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc tctcctccag
     9241 ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga tcattcgttt
     9301 catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg atcatatgtt
     9361 gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca aaaggatctt
     9421 tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt ttcttttcat
     9481 gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc ttttgataat
     9541 tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat ggatatgtaa
     9601 aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa agactgattt
     9661 gggtttgagg tctattacct attttctttt tcattttctt caccttctta tttaaaaaaa
     9721 ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta tacttcagac
     9781 ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat tccgattagt
     9841 aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt catattcggt
     9901 actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt atagcttcca
     9961 taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata accttctggc
    10021 gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc aaagtatcca
    10081 tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat tctatctgct
    10141 cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta ttcagaacgg
    10201 tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga tgcatcatat
    10261 ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc ttcttcataa
    10321 tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa atcatccgct
    10381 tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc acgtgcatct
    10441 gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa aatcgtcacg
    10501 acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa aatcgtctcg
    10561 ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg cgtatctgtg
    10621 attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat gaaataagga
    10681 ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat ataagaaatc
    10741 gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct ttcctctaca
    10801 ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac atctgtattg
    10861 tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg catcatcact
    10921 ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga ataagtttct
    10981 aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg gacggtagag
    11041 aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag tcgatatttt
    11101 aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc agaattaaga
    11161 ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata tacttccgtt
    11221 acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc cgtagaaacc
    11281 aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg taatgtaatc
    11341 gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc gttcccgaaa
    11401 atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat cattttttta
    11461 ttcatttttt tctccccgtt ctttttttat t
//
LOCUS       DARDHD010000084        11269 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000084 DARDHD010000000
VERSION     DARDHD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.084"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(303..2312)
                     /gene="metG"
                     /locus_tag="SDA06_001714"
     CDS             complement(303..2312)
                     /gene="metG"
                     /locus_tag="SDA06_001714"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG4228988.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2580..2858
                     /locus_tag="SDA06_001715"
     CDS             2580..2858
                     /locus_tag="SDA06_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4228989.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2955..3776
                     /locus_tag="SDA06_001716"
     CDS             2955..3776
                     /locus_tag="SDA06_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4228990.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4061..5071)
                     /locus_tag="SDA06_001717"
     CDS             complement(4061..5071)
                     /locus_tag="SDA06_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4228991.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5083..6036)
                     /locus_tag="SDA06_001718"
     CDS             complement(5083..6036)
                     /locus_tag="SDA06_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4228992.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6026..7096)
                     /locus_tag="SDA06_001719"
     CDS             complement(6026..7096)
                     /locus_tag="SDA06_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4228993.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7102..8145)
                     /locus_tag="SDA06_001720"
     CDS             complement(7102..8145)
                     /locus_tag="SDA06_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228994.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8146..9087)
                     /locus_tag="SDA06_001721"
     CDS             complement(8146..9087)
                     /locus_tag="SDA06_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4228995.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9171..9518
                     /locus_tag="SDA06_001722"
     CDS             9171..9518
                     /locus_tag="SDA06_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG4228996.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9588..11249)
                     /locus_tag="SDA06_001723"
     CDS             complement(9588..11249)
                     /locus_tag="SDA06_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4228997.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3165 a         2301 c         2057 g         3746 t
ORIGIN      
        1 ttgcattcaa actttatgaa tgtaaggaca ataaaaaaga gtaccagatg aatcaattaa
       61 aatctgcccg accctttatc attatgcttt ttttctgttt ttacgattcc tattagtatt
      121 attctaaatt aacaaaaatc agcgaagctc gtacttcttt ttctaggatt tattttggat
      181 agttctctgt ttctgtatta tatgattggc ttcaagtaat aagaaggaat ttacgaaaat
      241 tgcttctcaa atttcggaaa aagggggtat gacacaacta gtgtcatacc ccctttgaat
      301 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
      361 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      421 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      481 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      541 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      601 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      661 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      721 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      781 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      841 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      901 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      961 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
     1021 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
     1081 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
     1141 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
     1201 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
     1261 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1321 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1381 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1441 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1501 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1561 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1621 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1681 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1741 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1801 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1861 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1921 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1981 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     2041 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     2101 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     2161 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     2221 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     2281 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2341 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2401 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2461 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2521 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2581 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2641 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2701 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2761 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2821 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2881 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2941 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     3001 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     3061 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     3121 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     3181 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     3241 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3301 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3361 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3421 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3481 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3541 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3601 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3661 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3721 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3781 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3841 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3901 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3961 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     4021 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     4081 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     4141 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     4201 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     4261 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4321 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4381 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4441 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4501 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4561 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4621 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4681 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4741 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4801 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4861 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4921 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4981 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     5041 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     5101 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     5161 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     5221 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     5281 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5341 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5401 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5461 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5521 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5581 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5641 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5701 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5761 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5821 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5881 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5941 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     6001 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     6061 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     6121 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     6181 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     6241 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6301 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6361 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6421 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6481 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6541 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6601 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6661 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6721 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6781 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6841 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6901 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6961 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     7021 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     7081 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     7141 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     7201 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     7261 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7321 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7381 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7441 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7501 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7561 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7621 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7681 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7741 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7801 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7861 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7921 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7981 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     8041 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     8101 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     8161 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     8221 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     8281 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8341 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8401 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8461 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8521 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8581 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8641 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8701 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8761 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8821 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8881 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8941 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     9001 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     9061 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     9121 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     9181 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     9241 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9301 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9361 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9421 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9481 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9541 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9601 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9661 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9721 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9781 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9841 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9901 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9961 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
    10021 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
    10081 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
    10141 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
    10201 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
    10261 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10321 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10381 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10441 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10501 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10561 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10621 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10681 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10741 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10801 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10861 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10921 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10981 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    11041 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    11101 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    11161 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    11221 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgg
//
LOCUS       DARDHD010000085        11108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000085 DARDHD010000000
VERSION     DARDHD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.085"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            70..1329
                     /gene="lysA"
                     /locus_tag="SDA06_001724"
     CDS             70..1329
                     /gene="lysA"
                     /locus_tag="SDA06_001724"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG4228998.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1316..2116
                     /gene="dapF"
                     /locus_tag="SDA06_001725"
     CDS             1316..2116
                     /gene="dapF"
                     /locus_tag="SDA06_001725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG4228999.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2119..3000
                     /locus_tag="SDA06_001726"
     CDS             2119..3000
                     /locus_tag="SDA06_001726"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG4229000.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2997..3713
                     /locus_tag="SDA06_001727"
     CDS             2997..3713
                     /locus_tag="SDA06_001727"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG4229001.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3719..4414
                     /gene="dapD"
                     /locus_tag="SDA06_001728"
     CDS             3719..4414
                     /gene="dapD"
                     /locus_tag="SDA06_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG4229002.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4430..5431
                     /locus_tag="SDA06_001729"
     CDS             4430..5431
                     /locus_tag="SDA06_001729"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG4229003.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5428..6789
                     /locus_tag="SDA06_001730"
     CDS             5428..6789
                     /locus_tag="SDA06_001730"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG4229004.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6749..7900
                     /locus_tag="SDA06_001731"
     CDS             6749..7900
                     /locus_tag="SDA06_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG4229005.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7875..9044
                     /locus_tag="SDA06_001732"
     CDS             7875..9044
                     /locus_tag="SDA06_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4229006.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9219..10598
                     /locus_tag="SDA06_001733"
     CDS             9219..10598
                     /locus_tag="SDA06_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4229007.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10879..11108
                     /locus_tag="SDA06_001734"
                     /pseudo
     CDS             10879..11108
                     /locus_tag="SDA06_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3695 a         1944 c         2494 g         2975 t
ORIGIN      
        1 taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg
       61 aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag
      121 tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc
      181 aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc taaagcattc
      241 caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt
      301 ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat ctacttccat
      361 ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt
      421 gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa
      481 aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc
      541 gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa
      601 gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca tgctcatatc
      661 gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac
      721 ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat
      781 acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg
      841 gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga accgggtaga
      901 agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca
      961 cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc agctctgtat
     1021 caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg
     1081 acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg
     1141 aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg
     1201 agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca
     1261 agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat
     1321 ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac
     1381 agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata cagacggatt
     1441 gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc
     1501 gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat
     1561 gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga
     1621 tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat
     1681 caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt
     1741 aacgatcact agtttatttt taggaacgat tcatacagtt gtttttgttg aaaatgcgcg
     1801 tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa
     1861 aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga
     1921 aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc
     1981 taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt
     2041 gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag
     2101 aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat
     2161 gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa
     2221 caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct
     2281 gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt
     2341 gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct
     2401 ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga
     2461 ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat
     2521 gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat
     2581 cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct
     2641 cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta
     2701 atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc
     2761 catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta
     2821 aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca
     2881 gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca
     2941 aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga
     3001 aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa
     3061 gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta
     3121 cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt
     3181 cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat acacaatcac
     3241 agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt
     3301 cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct
     3361 ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg
     3421 caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc
     3481 aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac
     3541 ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat
     3601 ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc
     3661 agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt
     3721 ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt
     3781 ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag
     3841 cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga
     3901 aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga
     3961 tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat
     4021 cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga
     4081 aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg
     4141 ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt
     4201 tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat
     4261 cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcagaaac
     4321 tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa
     4381 tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat
     4441 agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga
     4501 agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa
     4561 agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga
     4621 aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat
     4681 cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga
     4741 tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc
     4801 aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt
     4861 tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg
     4921 cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc cttataatat
     4981 tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga
     5041 acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc
     5101 aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca
     5161 aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt
     5221 agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat
     5281 ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac
     5341 gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt
     5401 aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc
     5461 ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat
     5521 cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa
     5581 gtaacagact tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc
     5641 ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta
     5701 cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa
     5761 gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt
     5821 taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag
     5881 gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat ggttgttcca
     5941 gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac
     6001 attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat
     6061 gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa
     6121 atctctttta gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca
     6181 gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct
     6241 ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga
     6301 atagctggac gcaaggattt tctttccatc actttattca aacgtcatat gtccgatgaa
     6361 atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat
     6421 attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataag
     6481 ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatc
     6541 gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat tggattatct
     6601 gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga
     6661 gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac agtagtgaag
     6721 ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca
     6781 aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa
     6841 aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa
     6901 tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga
     6961 aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg gtcatgacgg
     7021 acatatggcg atgcttttag ggttgactga ctttataact aaacacattg aggatcttat
     7081 ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt
     7141 tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg
     7201 gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag
     7261 tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat
     7321 cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt
     7381 accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgcaa
     7441 tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt cagatatgca
     7501 aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta cagctactat
     7561 ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc
     7621 actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt
     7681 tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc
     7741 tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg
     7801 gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga
     7861 ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt tctagaatag
     7921 aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac
     7981 tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca ggaatagcag
     8041 cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag
     8101 cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt
     8161 tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag
     8221 gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag
     8281 ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt cagccggaac
     8341 aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc
     8401 ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt
     8461 taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg
     8521 gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat
     8581 ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta
     8641 ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa
     8701 aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat
     8761 atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc
     8821 agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt
     8881 ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat
     8941 ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac
     9001 atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata gattttatca
     9061 aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt tttaaattta
     9121 gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt taaattgttt
     9181 agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca
     9241 ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca cgatacggtg
     9301 aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg tcatgtggta
     9361 tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat ttcagaccag
     9421 aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa acgatcgatg
     9481 ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa gaatatcctt
     9541 atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta ccaggagcag
     9601 atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc aaagaaaaat
     9661 taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag
     9721 cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg atcgatagac
     9781 ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac ctaaatgaaa
     9841 acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg
     9901 aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa gctgccggtg
     9961 tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc
    10021 aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga gatgctacac
    10081 ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta
    10141 gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg
    10201 gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca agcggattga
    10261 atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat gaagatacgt
    10321 tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc caattattct
    10381 cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat gatgttacag
    10441 gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa gaactagcag
    10501 aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg aatctagccg
    10561 ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata tttgaaaaga
    10621 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa acagcttctt
    10681 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattt
    10741 tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc
    10801 taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta aattaaagat
    10861 cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga tcaagagatt
    10921 gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt acggatgacc
    10981 aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg cttgatcaag
    11041 aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga
    11101 ggaaaagt
//
LOCUS       DARDHD010000086        10924 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000086 DARDHD010000000
VERSION     DARDHD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.086"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SDA06_001735"
     CDS             complement(<1..281)
                     /locus_tag="SDA06_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229008.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SDA06_001736"
     CDS             423..1541
                     /locus_tag="SDA06_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4229009.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SDA06_001737"
     CDS             1669..3045
                     /locus_tag="SDA06_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4229010.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SDA06_001738"
     CDS             3446..5026
                     /locus_tag="SDA06_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG4229011.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SDA06_001739"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SDA06_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SDA06_001740"
     CDS             complement(5652..5972)
                     /locus_tag="SDA06_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG4229012.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SDA06_001741"
     CDS             complement(6120..8354)
                     /locus_tag="SDA06_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229013.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SDA06_001742"
     CDS             8833..9099
                     /locus_tag="SDA06_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG4229014.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SDA06_001743"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SDA06_001743"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG4229015.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1911 c         2215 g         3318 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aaat
//
LOCUS       DARDHD010000087        10899 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000087 DARDHD010000000
VERSION     DARDHD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.087"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..666
                     /locus_tag="SDA06_001744"
     CDS             <1..666
                     /locus_tag="SDA06_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4229016.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="SDA06_001745"
     CDS             678..1598
                     /locus_tag="SDA06_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4229017.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="SDA06_001746"
     CDS             1639..3096
                     /locus_tag="SDA06_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4229018.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="SDA06_001747"
     CDS             3441..4055
                     /locus_tag="SDA06_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4229019.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="SDA06_001748"
     CDS             4048..5772
                     /locus_tag="SDA06_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4229020.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="SDA06_001749"
     CDS             5769..7220
                     /locus_tag="SDA06_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229021.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7693..9846)
                     /locus_tag="SDA06_001750"
     CDS             complement(7693..9846)
                     /locus_tag="SDA06_001750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG4229022.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9848..10894)
                     /locus_tag="SDA06_001751"
     CDS             complement(9848..10894)
                     /locus_tag="SDA06_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229023.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
BASE COUNT         3564 a         1924 c         2163 g         3248 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg agggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
     7381 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga
     7441 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa
     7501 atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac
     7561 aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga
     7621 aaaatatgag gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
     7681 cacaacctct tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt
     7741 cttagggtga gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg
     7801 atgggtaaaa tataatttcg tatattctct gtccatccgt ttgattttat ggataaattg
     7861 ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa
     7921 aacagccttg tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg
     7981 ataaaatccc agactattga agatatacca agcggccata cttccattgt cctcatctcc
     8041 agggtaaccc attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg
     8101 tgccatttca ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt
     8161 tgaaatagct aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa
     8221 accatatcct cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt
     8281 atatctctca ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa
     8341 gctgctttgc caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg
     8401 ttctctgaac ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat
     8461 attttgatag ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac
     8521 cttggcaact tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga
     8581 ttcgtggtag tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc
     8641 ataatttgga tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg
     8701 catcaagtct cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt
     8761 cccaggcata agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct
     8821 atagctgttc aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata
     8881 aagaggataa accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg
     8941 ttttatagat cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata
     9001 aaatgtttgt ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg
     9061 ttgatcatgg tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg
     9121 agcaaggtaa gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc
     9181 cgagctgata aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa
     9241 cgggatcgag tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc
     9301 aaatctcaat ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat
     9361 ttttccttcc actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa
     9421 aagtaaaccg ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg
     9481 tatcaatgta gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa
     9541 tatactttct tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt
     9601 tccattgatc ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg
     9661 ggtaagacgg tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg
     9721 atccgtagtt tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca
     9781 gttcccatta gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat
     9841 attcatttta tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat
     9901 cggatggtcg atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta
     9961 actgttcaaa atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga
    10021 tatcatcaaa tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga
    10081 gattcatggt ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact
    10141 tagtgatata agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga
    10201 aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt
    10261 gtggatgagt ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat
    10321 aagaggcagc aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact
    10381 catagatttt tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta
    10441 agtcaagatg ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta
    10501 gctcttttgc tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt
    10561 tatcttgcat cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat
    10621 atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag
    10681 aagcggattt aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta
    10741 attcagtcaa tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt
    10801 tttcagttgg tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat
    10861 cattaaatat tgtctggtac aatggttgag tcatataaa
//
LOCUS       DARDHD010000088        10723 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000088 DARDHD010000000
VERSION     DARDHD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.088"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(241..1089)
                     /locus_tag="SDA06_001752"
     CDS             complement(241..1089)
                     /locus_tag="SDA06_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4229024.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="SDA06_001753"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="SDA06_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG4229025.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="SDA06_001754"
     CDS             2157..3158
                     /locus_tag="SDA06_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG4229026.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="SDA06_001755"
     CDS             3155..3439
                     /locus_tag="SDA06_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG4229027.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="SDA06_001756"
     CDS             3472..4269
                     /locus_tag="SDA06_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4229028.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="SDA06_001757"
     CDS             4283..5116
                     /locus_tag="SDA06_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229029.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="SDA06_001758"
     CDS             5134..5991
                     /locus_tag="SDA06_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4229030.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="SDA06_001759"
     CDS             6013..6522
                     /locus_tag="SDA06_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229031.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="SDA06_001760"
     CDS             6559..6960
                     /locus_tag="SDA06_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229032.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="SDA06_001761"
     CDS             7072..7425
                     /locus_tag="SDA06_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229033.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="SDA06_001762"
     CDS             7459..7902
                     /locus_tag="SDA06_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4229034.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="SDA06_001763"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="SDA06_001763"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4229035.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="SDA06_001764"
     CDS             8997..9710
                     /locus_tag="SDA06_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4229036.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="SDA06_001765"
     CDS             complement(9917..10066)
                     /locus_tag="SDA06_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229037.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="SDA06_001766"
     CDS             10462..10662
                     /locus_tag="SDA06_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4229038.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DARDHD010000089        10489 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000089 DARDHD010000000
VERSION     DARDHD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.089"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            34..657
                     /locus_tag="SDA06_001767"
     CDS             34..657
                     /locus_tag="SDA06_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229039.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="SDA06_001768"
     CDS             1107..2156
                     /locus_tag="SDA06_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4229040.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="SDA06_001769"
     CDS             2153..3034
                     /locus_tag="SDA06_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4229041.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="SDA06_001770"
     CDS             3037..3807
                     /locus_tag="SDA06_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4229042.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="SDA06_001771"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="SDA06_001771"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG4229043.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="SDA06_001772"
     CDS             5118..6281
                     /locus_tag="SDA06_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4229044.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="SDA06_001773"
     CDS             6398..6808
                     /locus_tag="SDA06_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4229045.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="SDA06_001774"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="SDA06_001774"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4229046.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="SDA06_001775"
     CDS             9381..9665
                     /locus_tag="SDA06_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229047.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="SDA06_001776"
     CDS             9825..10061
                     /locus_tag="SDA06_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4229048.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="SDA06_001777"
     CDS             10058..10456
                     /locus_tag="SDA06_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4229049.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3658 a         1764 c         2222 g         2845 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatca
//
LOCUS       DARDHD010000090        10288 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000090 DARDHD010000000
VERSION     DARDHD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.090"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SDA06_001778"
     CDS             6..305
                     /locus_tag="SDA06_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229050.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SDA06_001779"
     CDS             378..1739
                     /locus_tag="SDA06_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG4229051.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SDA06_001780"
     CDS             1761..2021
                     /locus_tag="SDA06_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229052.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SDA06_001781"
     CDS             2035..2787
                     /locus_tag="SDA06_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG4229053.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SDA06_001782"
     CDS             complement(2881..3486)
                     /locus_tag="SDA06_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4229054.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SDA06_001783"
     CDS             complement(3539..4513)
                     /locus_tag="SDA06_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG4229055.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SDA06_001784"
     CDS             complement(4891..5610)
                     /locus_tag="SDA06_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4229056.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SDA06_001785"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SDA06_001785"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4229057.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SDA06_001786"
     CDS             7161..8558
                     /locus_tag="SDA06_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4229058.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SDA06_001787"
     CDS             8639..9079
                     /locus_tag="SDA06_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4229059.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SDA06_001788"
     CDS             9227..9553
                     /locus_tag="SDA06_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4229060.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..>10288
                     /locus_tag="SDA06_001789"
     CDS             9554..>10288
                     /locus_tag="SDA06_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229061.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQS"
BASE COUNT         3262 a         1817 c         2134 g         3075 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagc
//
LOCUS       DARDHD010000091        10264 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000091 DARDHD010000000
VERSION     DARDHD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.091"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1565)
                     /locus_tag="SDA06_001790"
     CDS             complement(57..1565)
                     /locus_tag="SDA06_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG4229062.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1724..1915
                     /locus_tag="SDA06_001791"
     CDS             1724..1915
                     /locus_tag="SDA06_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229063.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2118..3035
                     /locus_tag="SDA06_001792"
     CDS             2118..3035
                     /locus_tag="SDA06_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG4229064.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3028..4101
                     /locus_tag="SDA06_001793"
     CDS             3028..4101
                     /locus_tag="SDA06_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229065.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4249..4767
                     /locus_tag="SDA06_001794"
     CDS             4249..4767
                     /locus_tag="SDA06_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229066.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4748..5140
                     /locus_tag="SDA06_001795"
     CDS             4748..5140
                     /locus_tag="SDA06_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG4229067.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5216..6151
                     /locus_tag="SDA06_001796"
     CDS             5216..6151
                     /locus_tag="SDA06_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG4229068.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6284..7063
                     /locus_tag="SDA06_001797"
     CDS             6284..7063
                     /locus_tag="SDA06_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4229069.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7056..7373
                     /locus_tag="SDA06_001798"
     CDS             7056..7373
                     /locus_tag="SDA06_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4229070.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7617..9227
                     /locus_tag="SDA06_001799"
     CDS             7617..9227
                     /locus_tag="SDA06_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4229071.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9384..10232
                     /locus_tag="SDA06_001800"
     CDS             9384..10232
                     /locus_tag="SDA06_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4229072.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3227 a         1800 c         2071 g         3166 t
ORIGIN      
        1 cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat
       61 tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt
      121 ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca
      181 agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat
      241 aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat
      301 ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata
      361 tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat caatgaactt
      421 gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct
      481 tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt
      541 cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct
      601 tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct
      661 tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca
      721 cccatgtcag taggagattg ataaacagat tctttaccag taattttttc aataatacgt
      781 ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca
      841 tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa
      901 gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag
      961 taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagca agtagcgagc
     1021 tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac
     1081 tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg
     1141 atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc
     1201 gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa gccataacca
     1261 cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg
     1321 atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc
     1381 aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca
     1441 tgcttgtcgt caactaactt cccaccaaat tctaaatata atttgtcata gtggttcact
     1501 cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt
     1561 ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa
     1621 ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat
     1681 agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa
     1741 aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct
     1801 tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac
     1861 tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat
     1921 aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa
     1981 aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta
     2041 taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt
     2101 aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac
     2161 tagaggagac gaacgcggat caggatacag gacaagaatc gtcttctttt tacgatatca
     2221 aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag
     2281 ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag
     2341 aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc
     2401 atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga
     2461 tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa
     2521 gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac
     2581 cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc
     2641 agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt
     2701 tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag
     2761 agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc
     2821 ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc
     2881 gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg
     2941 gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata
     3001 atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa
     3061 aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt
     3121 aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat acatgaaaaa
     3181 gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact
     3241 gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt
     3301 gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat
     3361 tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg
     3421 atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat
     3481 attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc
     3541 aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat
     3601 caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc
     3661 aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc
     3721 tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga
     3781 acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat
     3841 cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg
     3901 aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat
     3961 aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca
     4021 tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca
     4081 ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc
     4141 tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta
     4201 ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat
     4261 aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa
     4321 tttattccga atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc
     4381 ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt
     4441 gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca
     4501 atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt
     4561 ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg
     4621 cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc
     4681 aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga
     4741 ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt
     4801 gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac
     4861 atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt
     4921 ggtcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga
     4981 agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta
     5041 ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa
     5101 tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa
     5161 agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga
     5221 aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt
     5281 tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag
     5341 catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc
     5401 caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag
     5461 tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt
     5521 tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact
     5581 gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga
     5641 attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat
     5701 actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg
     5761 aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat
     5821 cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat
     5881 cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga
     5941 gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc
     6001 agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat
     6061 tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga
     6121 taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat
     6181 gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca
     6241 atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag
     6301 tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt
     6361 aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact
     6421 gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac
     6481 gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt
     6541 attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt
     6601 ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc
     6661 ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg
     6721 tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca
     6781 ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact
     6841 gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa
     6901 tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa
     6961 ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc
     7021 gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt
     7081 tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta
     7141 ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat
     7201 ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga
     7261 atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg
     7321 cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt
     7381 tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca
     7441 tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg
     7501 cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt
     7561 ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga
     7621 ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt
     7681 taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc
     7741 ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta
     7801 cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata
     7861 acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg
     7921 cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta
     7981 acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa
     8041 ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt
     8101 ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc
     8161 gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct
     8221 tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg
     8281 aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg
     8341 tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc
     8401 aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag
     8461 atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga
     8521 tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta agcacgatga
     8581 agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat
     8641 tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt
     8701 tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt
     8761 ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg
     8821 cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag
     8881 gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg
     8941 ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca
     9001 attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt
     9061 tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg
     9121 tggctgtagg aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt
     9181 ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt
     9241 tttttgctgt cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt
     9301 ccaattttag tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc
     9361 tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc
     9421 tgccggaacg tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg
     9481 atgggaaaat ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa
     9541 aaaacagtaa agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat
     9601 atgaggaatt gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga
     9661 tttctggctt tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca
     9721 ttccttatga ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag
     9781 acttaaatga tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag
     9841 ataataccta tgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt
     9901 tgacaaatgg tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt
     9961 ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag
    10021 ctgaagaaat tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga
    10081 tacatgcaaa taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc
    10141 caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa
    10201 aagccatcgg cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa
    10261 atat
//
LOCUS       DARDHD010000092        10214 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000092 DARDHD010000000
VERSION     DARDHD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.092"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            134..283
                     /locus_tag="SDA06_001801"
     CDS             134..283
                     /locus_tag="SDA06_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG4229073.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            294..1808
                     /gene="dltA"
                     /locus_tag="SDA06_001802"
     CDS             294..1808
                     /gene="dltA"
                     /locus_tag="SDA06_001802"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG4229074.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1805..3010
                     /gene="dltB"
                     /locus_tag="SDA06_001803"
     CDS             1805..3010
                     /gene="dltB"
                     /locus_tag="SDA06_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG4229075.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3055..3288
                     /gene="dltC"
                     /locus_tag="SDA06_001804"
     CDS             3055..3288
                     /gene="dltC"
                     /locus_tag="SDA06_001804"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG4229076.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3291..4559
                     /gene="dltD"
                     /locus_tag="SDA06_001805"
     CDS             3291..4559
                     /gene="dltD"
                     /locus_tag="SDA06_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG4229077.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4700..5782
                     /locus_tag="SDA06_001806"
     CDS             4700..5782
                     /locus_tag="SDA06_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG4229078.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5782..6585
                     /locus_tag="SDA06_001807"
     CDS             5782..6585
                     /locus_tag="SDA06_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4229079.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6655..7395
                     /locus_tag="SDA06_001808"
     CDS             6655..7395
                     /locus_tag="SDA06_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4229080.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7406..8410
                     /locus_tag="SDA06_001809"
     CDS             7406..8410
                     /locus_tag="SDA06_001809"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG4229081.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8455..10152)
                     /locus_tag="SDA06_001810"
     CDS             complement(8455..10152)
                     /locus_tag="SDA06_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229082.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3272 a         1694 c         2074 g         3174 t
ORIGIN      
        1 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
       61 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
      121 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
      181 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
      241 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
      301 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
      361 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
      421 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
      481 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
      541 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
      601 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
      661 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
      721 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
      781 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
      841 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
      901 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
      961 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
     1021 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
     1081 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
     1141 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
     1201 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
     1261 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
     1321 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
     1381 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
     1441 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
     1501 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
     1561 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
     1621 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
     1681 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
     1741 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
     1801 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
     1861 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
     1921 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
     1981 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
     2041 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
     2101 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
     2161 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
     2221 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
     2281 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
     2341 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
     2401 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
     2461 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
     2521 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
     2581 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
     2641 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
     2701 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
     2761 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
     2821 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
     2881 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
     2941 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
     3001 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
     3061 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
     3121 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
     3181 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
     3241 agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
     3301 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
     3361 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
     3421 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
     3481 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
     3541 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
     3601 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
     3661 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
     3721 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
     3781 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
     3841 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
     3901 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
     3961 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
     4021 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
     4081 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
     4141 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
     4201 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
     4261 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
     4321 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
     4381 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
     4441 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
     4501 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
     4561 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
     4621 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
     4681 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
     4741 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
     4801 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
     4861 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
     4921 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
     4981 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
     5041 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
     5101 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
     5161 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
     5221 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
     5281 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
     5341 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
     5401 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
     5461 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
     5521 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
     5581 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
     5641 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
     5701 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
     5761 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
     5821 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
     5881 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
     5941 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
     6001 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
     6061 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
     6121 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
     6181 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
     6241 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
     6301 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
     6361 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
     6421 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
     6481 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
     6541 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
     6601 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
     6661 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
     6721 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
     6781 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
     6841 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
     6901 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
     6961 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
     7021 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
     7081 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
     7141 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
     7201 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
     7261 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
     7321 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
     7381 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
     7441 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
     7501 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
     7561 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
     7621 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
     7681 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
     7741 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
     7801 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
     7861 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
     7921 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
     7981 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
     8041 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
     8101 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
     8161 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
     8221 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
     8281 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
     8341 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
     8401 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
     8461 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
     8521 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
     8581 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
     8641 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
     8701 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
     8761 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
     8821 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
     8881 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
     8941 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
     9001 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
     9061 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
     9121 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
     9181 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
     9241 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
     9301 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
     9361 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
     9421 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
     9481 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
     9541 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
     9601 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
     9661 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
     9721 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
     9781 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
     9841 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
     9901 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
     9961 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    10021 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    10081 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    10141 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    10201 tcctgttaaa aata
//
LOCUS       DARDHD010000093        10192 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000093 DARDHD010000000
VERSION     DARDHD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.093"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..625
                     /locus_tag="SDA06_001811"
     CDS             119..625
                     /locus_tag="SDA06_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG4229083.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="SDA06_001812"
     CDS             642..1541
                     /locus_tag="SDA06_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG4229084.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="SDA06_001813"
     CDS             1628..2512
                     /locus_tag="SDA06_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4229085.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="SDA06_001814"
     tRNA            complement(2704..2775)
                     /locus_tag="SDA06_001814"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="SDA06_001815"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="SDA06_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG4229086.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="SDA06_001816"
     CDS             4318..5409
                     /locus_tag="SDA06_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG4229087.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="SDA06_001817"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="SDA06_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4229088.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="SDA06_001818"
     CDS             6561..7751
                     /locus_tag="SDA06_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG4229089.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="SDA06_001819"
     CDS             7855..8610
                     /locus_tag="SDA06_001819"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG4229090.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="SDA06_001820"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="SDA06_001820"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG4229091.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DARDHD010000094        10091 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000094 DARDHD010000000
VERSION     DARDHD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.094"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SDA06_001821"
     CDS             162..392
                     /locus_tag="SDA06_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG4229092.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SDA06_001822"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SDA06_001822"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4229093.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SDA06_001823"
     CDS             1803..1979
                     /locus_tag="SDA06_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4229094.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SDA06_001824"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SDA06_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG4229095.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SDA06_001825"
     CDS             2474..2941
                     /locus_tag="SDA06_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229096.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SDA06_001826"
     CDS             3026..3910
                     /locus_tag="SDA06_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4229097.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SDA06_001827"
     CDS             4024..4260
                     /locus_tag="SDA06_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG4229098.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SDA06_001828"
     tRNA            4348..4428
                     /locus_tag="SDA06_001828"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SDA06_001829"
     tRNA            4435..4506
                     /locus_tag="SDA06_001829"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SDA06_001830"
     CDS             4631..4801
                     /locus_tag="SDA06_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229099.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SDA06_001831"
     CDS             5021..5728
                     /locus_tag="SDA06_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4229100.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SDA06_001832"
     CDS             complement(5788..6432)
                     /locus_tag="SDA06_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG4229101.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SDA06_001833"
     CDS             6559..6846
                     /locus_tag="SDA06_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229102.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SDA06_001834"
     CDS             6954..7274
                     /locus_tag="SDA06_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229103.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SDA06_001835"
     CDS             7355..8668
                     /locus_tag="SDA06_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4229104.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SDA06_001836"
     CDS             8665..9384
                     /locus_tag="SDA06_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG4229105.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SDA06_001837"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SDA06_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG4229106.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1670 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa c
//
LOCUS       DARDHD010000095        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000095 DARDHD010000000
VERSION     DARDHD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.095"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SDA06_001838"
     CDS             complement(247..759)
                     /locus_tag="SDA06_001838"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG4229107.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDA06_001839"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDA06_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG4229108.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SDA06_001840"
     CDS             complement(3212..3655)
                     /locus_tag="SDA06_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG4229109.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SDA06_001841"
     CDS             complement(3656..4444)
                     /locus_tag="SDA06_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4229110.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SDA06_001842"
     CDS             complement(4441..5382)
                     /locus_tag="SDA06_001842"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG4229111.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SDA06_001843"
     CDS             complement(5559..6146)
                     /locus_tag="SDA06_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG4229112.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDA06_001844"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDA06_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG4229113.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SDA06_001845"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SDA06_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4229114.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SDA06_001846"
     CDS             complement(9063..9998)
                     /locus_tag="SDA06_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4229115.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARDHD010000096        10047 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000096 DARDHD010000000
VERSION     DARDHD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.096"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SDA06_001847"
     CDS             complement(25..162)
                     /locus_tag="SDA06_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4229116.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SDA06_001848"
     CDS             complement(159..1343)
                     /locus_tag="SDA06_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG4229117.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SDA06_001849"
     CDS             complement(1599..1853)
                     /locus_tag="SDA06_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229118.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SDA06_001850"
     CDS             complement(1878..3020)
                     /locus_tag="SDA06_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG4229119.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SDA06_001851"
     CDS             complement(3080..4432)
                     /locus_tag="SDA06_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4229120.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SDA06_001852"
     CDS             complement(4503..4847)
                     /locus_tag="SDA06_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4229121.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SDA06_001853"
     CDS             complement(4857..5231)
                     /locus_tag="SDA06_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4229122.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SDA06_001854"
     CDS             complement(5244..5558)
                     /locus_tag="SDA06_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4229123.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDA06_001855"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDA06_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SDA06_001856"
     CDS             complement(8976..9638)
                     /locus_tag="SDA06_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229124.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2706 a         2268 c         1716 g         3357 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccg
//
LOCUS       DARDHD010000097        10022 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000097 DARDHD010000000
VERSION     DARDHD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.097"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..821)
                     /locus_tag="SDA06_001857"
     CDS             complement(<1..821)
                     /locus_tag="SDA06_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4229125.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLK"
     gene            967..1770
                     /locus_tag="SDA06_001858"
     CDS             967..1770
                     /locus_tag="SDA06_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG4229126.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1948..2895
                     /locus_tag="SDA06_001859"
     CDS             1948..2895
                     /locus_tag="SDA06_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG4229127.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2963..3256)
                     /locus_tag="SDA06_001860"
     CDS             complement(2963..3256)
                     /locus_tag="SDA06_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG4229128.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3284..3949)
                     /locus_tag="SDA06_001861"
     CDS             complement(3284..3949)
                     /locus_tag="SDA06_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG4229129.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4089..4721)
                     /locus_tag="SDA06_001862"
     CDS             complement(4089..4721)
                     /locus_tag="SDA06_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229130.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4728..5795)
                     /locus_tag="SDA06_001863"
     CDS             complement(4728..5795)
                     /locus_tag="SDA06_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4229131.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5792..6523)
                     /locus_tag="SDA06_001864"
     CDS             complement(5792..6523)
                     /locus_tag="SDA06_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG4229132.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6692..7171)
                     /gene="greA"
                     /locus_tag="SDA06_001865"
     CDS             complement(6692..7171)
                     /gene="greA"
                     /locus_tag="SDA06_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG4229133.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7307..8482)
                     /gene="mltG"
                     /locus_tag="SDA06_001866"
     CDS             complement(7307..8482)
                     /gene="mltG"
                     /locus_tag="SDA06_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG4229134.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8686..9855
                     /locus_tag="SDA06_001867"
     CDS             8686..9855
                     /locus_tag="SDA06_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4229135.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         3071 a         2021 c         1567 g         3363 t
ORIGIN      
        1 aattttaata aagaaattat acgaggatat atcttttcaa tctcgcttag tagctgatct
       61 tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca
      121 tgatgaacat cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg
      181 atagcaggtg cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct
      241 atcaatatat atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa
      301 aaacggatca aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa
      361 tgcaaataac tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact
      421 gagaacaaat tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc
      481 ttttctggtg aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc
      541 aatatgaatg agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt
      601 tttttatttg cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat
      661 gtatcactaa ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct
      721 ccaataaatt ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt
      781 gatgatctct gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt
      841 ctttagacaa gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag
      901 cacttgtatt tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga
      961 gtacttatga caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag
     1021 gaggcgagaa atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt
     1081 atggctttgc caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg
     1141 caaaagattg ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa
     1201 gaaaaagaag gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga
     1261 aaacgaacag aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg
     1321 ttaaaaaaca aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac
     1381 aataaccaat tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag
     1441 ctccgttctt ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata
     1501 ttttctattt atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct
     1561 gcttttctct atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca
     1621 ttgatctatt caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct
     1681 tcttttgaca ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc
     1741 aaaggatggg caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc
     1801 aatagaccct tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa
     1861 tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt
     1921 agagtaaaga aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca
     1981 tttcgtgatg aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca
     2041 gaattacaac cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac
     2101 caagaaaaac tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt
     2161 gatcagctac cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc
     2221 atccacagtg aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt
     2281 ctttttcaag cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac
     2341 caatctatcc aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt
     2401 gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca
     2461 gcagaaggaa tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta
     2521 gatttcgtag tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat
     2581 atgtttgaac actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt
     2641 aagacacaag atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac
     2701 gtttttgagg aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg
     2761 attacttccc acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt
     2821 ttaaccaatc tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg
     2881 tctgcaggat attaatagct gaaaacaata ttttcaaaag aaaaaagaga aggtgacaaa
     2941 aattgtgtca ccctctcttt ttttattgtt ttttaaagtg ataggttggc tcctccaggt
     3001 tgggatcata atcaacaagt aaatcatatc ctttaaagaa ccattcatct gcttcctcga
     3061 taaaaaaaag tgtcccatcc atttccactt tctttacagg atgttctggc tgttcaactg
     3121 acattccaat cgaaaaacca tcatgaacct ctgttttgcc gtagactttt ccaaaaaaac
     3181 gtattccgta agtttctggc agatcaattt ctgagtcaaa ccacttttgt gcttttggcg
     3241 ttaccgttaa tttcatccca acaacctact ttctcttttc tttctattga ttcatcttat
     3301 cgtctagaat atccacatcg cctgtctctc caataaccag tagattatca ttttcacgaa
     3361 catattcatc tgctgcagga gaagcgataa ccgtttgatc tactcttcgt atggcgacta
     3421 ctgttaagtc aaaacgttgc ctgaaattta attcagctaa tgttttgttg aaaaatttag
     3481 gattggagac tcggatttct gctaaagaat atttatctga caactctagg taatctaaaa
     3541 tattctttga cactaaatta tgcgcaatcc ttattcccat gtctcgctcg ggatggacga
     3601 cgcgatcagc tccgatcttt tcaagtactc tggcatgata ctcattttgg gcttttgcta
     3661 aaatatttgg tacgcccatt tcctttacca tcagagtaac taagatactg gcttgaatat
     3721 cttctccaat agcgacaatt acatgatcga aatttcttat gcctaatgat cgaagtgtca
     3781 tttcatcctg tgcatttgcc acaacagcat gagtggctat attcatatat tcattgaccc
     3841 gatcttcatt actatcaata gccagtactt cttgaccagc ttcgatcagt gttcggcaga
     3901 tacttccgcc aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc gttttcctcc
     3961 tttagtatct tcttttctca gtataacaaa gcctttacaa aaacaaaagc aagcatcatt
     4021 attgtaaaaa agccaagaaa aataatagtt attttctcag cttttgatta tcctctttat
     4081 catcgttttt atttcaccaa accatgtttg aatgcataga tagcggcttg tgtacggtct
     4141 tccacttcta gcttagccaa tatattggaa acatgtgttt ttactgtttt cagtgtaata
     4201 aacaattcgt ccgctatttc ctgattactt tttccttcag aaatcagcat taaaatttca
     4261 ttttcccgat tcgttagttc ttcatgtaat acaggctcat ttcgccgact cattttttcc
     4321 atcatttttg ttgtcacttc tggttctaat actcgctcgc cacgttgcgt tgcccttata
     4381 gcatcagcaa tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc acctgcttca
     4441 atggcaggat aaactttttc gtcatcaata aaactggtta cgataatgat tttagcttct
     4501 ggccaatctt ttaagattgc ttttgttgat tcgataccat ccatttcttc catgactaag
     4561 tccattaaaa tgacgtccgg tcgaagtgtc atcgccttct catagccttg tcgtccattt
     4621 tcagcttctc caattacttc aatgtcttct tgaatagata aataagaaga gacgcctaag
     4681 cgcaccattt catggtcatc tactaataaa acttttatca tccttatcta tgcctccttc
     4741 atcaaaggga ccttgatttc tacacttgta ccttgacctt taaaactaat gatttttact
     4801 gttccaccaa taccagctac tcgttctttg atattgttca atccataact tcccgttttc
     4861 gtttcattca tattaaaccc tgttccgtcg tcaataatac gtaacaaaag attattgtct
     4921 attttgtgca aatatacctc taattcattg gctttagcat gtcttaatgt atttgataac
     4981 aattcttgaa cgattcggaa caagtgatcc tcaatggaac tagatagttt tacatcttct
     5041 acatcccatt tcagcgaaat ctggatttta ttttgcaatt ccttcaataa ctgctcgata
     5101 ccttgcttta aacttttctc ttctaaattg actggacgta gatgaagaag cagcgcacgc
     5161 atctcagact gggaagcgtt gatgatctct gctaccattt tcaactgctt gcgaaacatt
     5221 tctggtgtct cgcttttttc tgcttgctcc gtcaatgccg acatcatcat cattgctgca
     5281 aacaattgtt gcgaaactga atcatgcagc tcacgggcta atcgatgtct ttctaattct
     5341 aaaatttctt ctttggtttg tccatctaca tattggggac ggctcgttac aatttgtaat
     5401 tcactagata cttcaatcat tttttctttt atcttagtga tttccttatc aatatctttg
     5461 atgtataaat cgtccgatgc acttgggatc ggaagaagca acaatttgga ttcataattg
     5521 ccagaagaaa gtgcacgcag cttttcttcg attttcccgt attttgtctt ttgaataagt
     5581 gataataata gaaagacaat taatcctaca cctaaggaaa tggctaacaa atggaagata
     5641 agcggaacat ataataaacg tgcacgcagt aattctcccc accaatgact ttgattgctg
     5701 gcataaaaat aagtgaaaag tgaaaataga ataataagga aagccgcaat ccccgaataa
     5761 acagctagca tcgctctgga tatttttccc atcatattcg tatcacctcg acatctccaa
     5821 tcaaggtatt agtaataatc ttcaatctac gagggttttc atcataatca ctgctgtaga
     5881 ttttgatttg ttcatttttc aaagaaaatt gctcttcttc aaacaagaca tttcctacta
     5941 atgtcgcatg ctctaatcgg atggctacgc ccaaaggtac taagatccgt gttctgccta
     6001 tcccttttct tacaatcaca atattatcat cttttggcaa aagcgtattg cccaaatcta
     6061 tgatagtatc accagaaatg acggcgatgt tgatatcgtc ccattcatag acgtcgtttc
     6121 caatacgttg gttaccgaat aattgctgtc ttttccgctc attattatgt gtttttaatt
     6181 gctctgtttg gatcatcatg atctgtttct ttcgccaaaa agcatttttg gttaaatcta
     6241 tccctgaaat ctctacacct ttcagaccaa taaataaaac ggcaaataca gccatcatcc
     6301 ataaagcagg actgttgact aagctaaaaa aaatgaccaa acttcctaat atcaattgaa
     6361 aattttgaaa tttagtacga ggatgtttgc gcatagctag atatatattg aaaataccga
     6421 atatcactaa aagcaaaagt ccagtattgt ttacgatttg ccaaacagca aataacagca
     6481 gtagtgcttc aactaccaca aaaaaacgcc aagaactatt catcgctctt tctccctcct
     6541 gacacattct catttcatca tacctatttt tttaactgtt ggctatagga ctttagatgt
     6601 aagatcgttt gttgaaatct aaaatacaca aagagaaaaa gatctagagt gtatcatcag
     6661 taagatgaac tctctagatc tttcagtcag actacgcttg ttctactttg acgattttaa
     6721 ctaacatatc gccacctgga gtagcaatag ttacttcatc atttttacgt ttgccaatca
     6781 atgcttgcgc aataggagaa tcattggaaa ttttgccaga aaacggatct gcttcagcgc
     6841 ttccgacaat cgtgtactct tcttcttctc catctggtag ttcgatgaat gtaactgttt
     6901 taccaattga tacctcatct gcatctacac cgtcgttatc gatgatttga gcaaaacgaa
     6961 tcatattttc aattgttgtg attcttcctt caacaaatgc ttgttcatct ttcgctgatt
     7021 catattcaga gttttcggaa agatctccga aaccgcgggc aattttgatt cgttcaatga
     7081 tttctccgcg tttgactgtt tttagttctt ctaattcttt ttctaatttt tctttacctt
     7141 ctagagtcat tggataaact ttctctacca tgtgtaacgt ctcctttttt cgataaaata
     7201 ccgcaaaagg taatcttttg aggaatatag tccaaaaaag attaccacgc ttttcttcaa
     7261 atgtaaatgt ttttctaaaa atgatcaatg tctagagcct gtttttctaa gaattattta
     7321 catatttttc cactaattcc atatgttctt cataagtttt agcgaaataa acatttccag
     7381 tgctcgtatc tgctacgaaa taataatagt cattttctgc tggttctaat actgccttga
     7441 ttgcctcttc gcttggattg tcaaaaggtc caggtccata gccggtattg gtatacaaat
     7501 tatatggtga atctacagct gtatcttcat acgtcactaa ttctttgtgt tcacctaatg
     7561 cgtataaaac agagatatct gactgaagtg gcatatttgc tttgattcgg ttaaagaaga
     7621 cttgtgcaat gtttttacga tcattttctt ttacgccttc tttttcaact agagaagcta
     7681 gagttaaaac ttcttgtaca gtcaaatttt tctgcttgat agctgaataa tagttttgca
     7741 tgaccgtatt cgtcttgttc accatttcaa tcactagatc cttaagcgaa gtactcgtgt
     7801 aataatcata tgtggctggg aataagtaac cttccaaacg gtattttgtg ttctcagctt
     7861 tagatgcact ttccaacagt tctgggaatt tttggtgcaa ctcgttaaag aaagtctcat
     7921 cattcactaa atctaaaaat tctttcttgt ctttaccagt taccttcgct acccgttcag
     7981 caatctgatc gatatcgtac ccttctggaa tggcaatttt cccatctgca acttttgttg
     8041 gctcagaagt accgccttct tgcaactgtt tgccaatctc gtcaagagtc atattaggcg
     8101 ctaactgata atagccagct tgaaagccag ttaaattttt aaattttgta taataattga
     8161 aaacaatccc gcttttaata atattgtcct tctccaaaat ctcaccaatc tgcttgttag
     8221 aagagccact cgggatctcg acttggacta attggtcatc tgatttatct aaaggcttca
     8281 acccgctatc tacataacga tacacggtaa atccaagaaa tccgccaata atcaataatg
     8341 taagagcaat cactaaaatg atcttcctca caatcttatc ttctttttta cgcagatttc
     8401 tccgcttgtc gattatatct gctgtagctg ccttactggc ttctttttta tctttacttg
     8461 ggtgttgttt gtttcctgac aaaacaactc ctccttacta tcaagttcgt aactttattc
     8521 attatacatg atatttgaat tggcgtgaat gattttattt aaatattcta tcaaaaatgt
     8581 aagtagtaaa tccattacta taagattaaa ataaatgtta tcaagataca agctatttta
     8641 gctttattct ttctgtaaaa aagaaaggag actaaataaa ctattatgaa aaaaggaaaa
     8701 catgctaaaa aaagaaaata ttcattttta atgatcgttg tcgtgttgtt tagccttagt
     8761 gcgttctcta caggaactat tcttggatgg caattttaca ataatgaaca ctctcagata
     8821 gttgcccatg ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga acaacagctc
     8881 gttgaaaaaa tcgatcaact attaagaaaa aatgattata ttggctcaat ttacgtaaag
     8941 aaaaataatc gcatcatcat ggaaaaaggc tatggctatg cgaataaact cacgcaacaa
     9001 ccaaatgatc cttctcttta ttatcagatt ggttcggttc aaaaagcatt gactactctt
     9061 ttgattttaa aacaagtcga gcgtgggcac ctttctttag atactaagct ggctcaattc
     9121 tatccacaaa ttgctggcag ccagcaaatt actatccgtg acttgctgta tatgcgctca
     9181 ggcttgcacc gaactgcttc tccaaccata ccgatgactg atgaagaagt cgttcaattt
     9241 gcgattcagc acttaaaatt tacagattat cagacctctc attatgattc attgaacttt
     9301 accatattga ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa attattgacc
     9361 gatgatatca tcgaaccttt aggattacag catacaaaat tttatgacca agtcaaaaat
     9421 tcatccaatc atgcactatc ctatcaaatg tctaaagagg atgattactt taaaccactg
     9481 acagaatctg aaacagagat ccataacgaa ttagggactg gtaatatcag tatgtccgtc
     9541 tatgatttaa atacgctgtt tacacaagtt ttatctggaa aactagtttc caaagactta
     9601 ctcttctcat tatggcgtga aaatgcgaat ggacatccat atagtggtgg ggtatatagc
     9661 ggagataatt atatcctgtc gcaaggaaat atcgactctt ttgatacatc tgctatattt
     9721 agcaaagatt cacaggatgc tatcatcatg gaaagcaata taaaagctga caaaactatc
     9781 aaactgccta ctgctgatct aagaaaccaa atctatgacc tgatggaagg aactactaag
     9841 ttggcaaata gctgatatga tgaaaagata gcaaatacta cttctttgaa tagcatttgc
     9901 ttaaaaaaag tactcgacta aacgtaggaa agagtgtggg acaaatctaa aaatcaattt
     9961 ttgctacaga ctttaattcc aaataaacag aacagaaaga ggtgttcttc tttctgttct
    10021 gt
//
LOCUS       DARDHD010000098         9920 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000098 DARDHD010000000
VERSION     DARDHD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.098"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(383..607)
                     /locus_tag="SDA06_001868"
     CDS             complement(383..607)
                     /locus_tag="SDA06_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229136.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            798..3575
                     /locus_tag="SDA06_001869"
     CDS             798..3575
                     /locus_tag="SDA06_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4229137.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3656..4075
                     /locus_tag="SDA06_001870"
     CDS             3656..4075
                     /locus_tag="SDA06_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG4229138.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4087..4557
                     /locus_tag="SDA06_001871"
     CDS             4087..4557
                     /locus_tag="SDA06_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229139.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4574..5353
                     /locus_tag="SDA06_001872"
     CDS             4574..5353
                     /locus_tag="SDA06_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229140.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5340..6161
                     /locus_tag="SDA06_001873"
     CDS             5340..6161
                     /locus_tag="SDA06_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4229141.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6178..7227
                     /locus_tag="SDA06_001874"
     CDS             6178..7227
                     /locus_tag="SDA06_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4229142.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7240..8244
                     /locus_tag="SDA06_001875"
     CDS             7240..8244
                     /locus_tag="SDA06_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4229143.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8877..9896
                     /locus_tag="SDA06_001876"
     CDS             8877..9896
                     /locus_tag="SDA06_001876"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4229144.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3324 a         1645 c         2074 g         2877 t
ORIGIN      
        1 atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg
       61 gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa
      121 aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt
      181 ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa
      241 aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca
      301 ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca
      361 ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca
      421 agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac
      481 gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag
      541 atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg
      601 atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa
      661 ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct
      721 ataataagat atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt
      781 tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga
      841 accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag
      901 ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg
      961 agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg
     1021 aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg
     1081 aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca
     1141 gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac
     1201 cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc
     1261 agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg
     1321 cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag
     1381 cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct
     1441 tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc
     1501 ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac
     1561 gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa
     1621 gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag
     1681 aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca
     1741 gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga
     1801 aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa
     1861 tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata
     1921 cagcacagca aggaatctat attacgcaag accagacctt agagcaatta atggagaagc
     1981 aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga
     2041 aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat
     2101 ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga
     2161 cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg
     2221 gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac
     2281 cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt
     2341 atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata
     2401 ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt
     2461 caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca
     2521 gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc
     2581 cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg
     2641 atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt
     2701 tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag
     2761 caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga
     2821 ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag
     2881 cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag
     2941 aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa
     3001 aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca
     3061 ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg
     3121 aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa
     3181 tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta
     3241 ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc
     3301 agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc
     3361 tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga
     3421 agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc
     3481 attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag
     3541 atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat
     3601 tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga
     3661 tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag attcattaga
     3721 gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt
     3781 tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt
     3841 tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga
     3901 tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt
     3961 ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat
     4021 gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga
     4081 agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt
     4141 tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg
     4201 aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc
     4261 aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta
     4321 ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc
     4381 aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg
     4441 gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa
     4501 gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa
     4561 aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca
     4621 atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac
     4681 aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc
     4741 atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat
     4801 tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg
     4861 tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc
     4921 aattatgaac cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca
     4981 tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc
     5041 ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca
     5101 aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg
     5161 tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta
     5221 cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt
     5281 caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga
     5341 tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt
     5401 ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg
     5461 tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag
     5521 ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag
     5581 gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga
     5641 tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt
     5701 ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata
     5761 ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc
     5821 tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg
     5881 gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa
     5941 tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg
     6001 tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg
     6061 gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag
     6121 ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg
     6181 tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag
     6241 aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg
     6301 ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt
     6361 actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc
     6421 gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa
     6481 atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt
     6541 gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt
     6601 acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg aattggaata
     6661 agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata
     6721 atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt
     6781 ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct
     6841 aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta
     6901 gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt
     6961 gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac
     7021 gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta
     7081 gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc
     7141 gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt
     7201 gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa
     7261 gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc
     7321 gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat
     7381 gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta
     7441 cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt
     7501 attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag
     7561 aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta
     7621 tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca
     7681 gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag
     7741 ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc
     7801 ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat
     7861 caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca
     7921 tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca
     7981 accccagtca cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct
     8041 aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta
     8101 gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg
     8161 acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc
     8221 tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag
     8281 agcagcgata actgctccct ttattttttt aaaatattgg catcaattac gttataaaac
     8341 acattttttg cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc
     8401 aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc
     8461 caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac
     8521 aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt
     8581 tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc
     8641 taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa
     8701 gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt
     8761 caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac
     8821 tttaagaaat gtatactata atggaaatta tcaattattt atttggagga atatccatga
     8881 aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg
     8941 cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca
     9001 ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg
     9061 gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa
     9121 caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca
     9181 ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg
     9241 ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg
     9301 ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag
     9361 cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg
     9421 atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg
     9481 ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg
     9541 cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga
     9601 gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg
     9661 gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg
     9721 ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga
     9781 tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag
     9841 caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag
     9901 atgatcaata gttaatcgtc
//
LOCUS       DARDHD010000099         9872 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000099 DARDHD010000000
VERSION     DARDHD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.099"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            89..1021
                     /locus_tag="SDA06_001877"
     CDS             89..1021
                     /locus_tag="SDA06_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229145.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1242..3005
                     /locus_tag="SDA06_001878"
     CDS             1242..3005
                     /locus_tag="SDA06_001878"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG4229146.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3089..3547
                     /locus_tag="SDA06_001879"
     CDS             3089..3547
                     /locus_tag="SDA06_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG4229147.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3656..4339
                     /locus_tag="SDA06_001880"
     CDS             3656..4339
                     /locus_tag="SDA06_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4229148.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4486..5100
                     /locus_tag="SDA06_001881"
     CDS             4486..5100
                     /locus_tag="SDA06_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG4229149.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5116..5322
                     /locus_tag="SDA06_001882"
     CDS             5116..5322
                     /locus_tag="SDA06_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229150.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5861..6418
                     /locus_tag="SDA06_001883"
                     /pseudo
     CDS             5861..6418
                     /locus_tag="SDA06_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6476..8515
                     /locus_tag="SDA06_001884"
     CDS             6476..8515
                     /locus_tag="SDA06_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG4229151.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(8624..9775)
                     /locus_tag="SDA06_001885"
     CDS             complement(8624..9775)
                     /locus_tag="SDA06_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG4229152.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         3550 a         1671 c         1975 g         2676 t
ORIGIN      
        1 gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa
       61 tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc atttttttaa
      121 aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg
      181 aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag aaacgaagcc
      241 gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag acttacaaga
      301 agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca atgagaacat
      361 ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc
      421 actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt
      481 tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt
      541 tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt
      601 agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt
      661 cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga
      721 aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt
      781 tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga
      841 agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag
      901 ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc
      961 aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg
     1021 atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta
     1081 tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt
     1141 aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg taacaaatct
     1201 attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca gtattacatg
     1261 tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac
     1321 agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg
     1381 ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta tttgattcac
     1441 caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag cttttaactg
     1501 gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat tccaaccaac
     1561 atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc agcttagcaa
     1621 caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta gcatttatcg
     1681 gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt ggtgttttac
     1741 aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag aatcatggca
     1801 gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc aataacctat
     1861 ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg gaaacggtga
     1921 tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac gtccatacgg
     1981 aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat tggcatgtac
     2041 catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca tttaatatga
     2101 tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt gccgcagtca
     2161 atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat ccagatcatt
     2221 attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct ttggcttctc
     2281 agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca tatgatcaat
     2341 tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt ggcggaacga
     2401 tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta tcaagtatcc
     2461 cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg ctagattggg
     2521 cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt gtagaacatg
     2581 gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat aatggaggaa
     2641 aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt
     2701 ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca gaaaatgacg
     2761 tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta gaagatggta
     2821 gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct tcaatgaacg
     2881 tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg cagctttatg
     2941 aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc
     3001 aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg atggtttttt
     3061 cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa
     3121 gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt catttgggga
     3181 tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa agactggtac
     3241 atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt
     3301 agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata taaatgtgac
     3361 catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg
     3421 gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt ataaagaagc
     3481 gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg aagtaatttt
     3541 cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag
     3601 ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag atattgtgga
     3661 tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc
     3721 gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag gcggagcagt
     3781 tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg ttgatttacc
     3841 ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta ctgcgacaga
     3901 aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg atgcaacttc
     3961 tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc acgaacacgg
     4021 tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg
     4081 ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa agacgcagac
     4141 acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag taattatgga
     4201 aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg ctttttcagt
     4261 cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg tcgaagccgc
     4321 aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt
     4381 gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt cattttgtct
     4441 tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt tgaacggagt
     4501 ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt catccctaga
     4561 ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc acccttcagt
     4621 tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca gagaaaacaa
     4681 gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt tatccatatc
     4741 cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc taatgaggaa
     4801 agcgaactgt acctgactga cttgacttta actgattcag taatcatttc agtaccagca
     4861 ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga gatacggaat
     4921 aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca tttaaatgaa
     4981 acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc tgtaagccgt
     5041 ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg tcccgaataa
     5101 aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc aaaagaaatg
     5161 aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga agttccgcca
     5221 ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat agaaatgcca
     5281 gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat tccgaaaagt
     5341 gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc tggtgcaact
     5401 gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa aaaatattaa
     5461 agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct
     5521 atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac
     5581 ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt
     5641 aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac
     5701 gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat
     5761 ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg
     5821 tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ggtcgctcgg attcacttct
     5881 ggttgcacaa tacaaccaga aaacacaaca agcaaaactg atctctatca tgagagattc
     5941 atatgtcgat attccaggtt acggaatgaa taagataaat gcagcttact cttatggggg
     6001 agttgattta ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc cctattatgc
     6061 aagtatcaca tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa
     6121 gatcgatgca gaaaaatctc tagatttaga tggcgtatat ataaacaaag gagagcaagt
     6181 gatggatggt aatactttac tccagtatgc tcgatttcgg gaagacaaag aaggagactt
     6241 cgggaggatc agaaggcagc agcaagtgat caaagctatc tctcagcagc tgaaagacgt
     6301 aacttcaata ttcaagctac ccaaagcggt tgggaaatta cttggtagta tccaaacgaa
     6361 ccttccagaa agtgtgcttc tggattgtgg tatggatttc ctcaaagaca tatgttagaa
     6421 taaacaggta taaatagtga aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa
     6481 aagaagtgac aagcacggca aaaatcgaac aggcgcttat attgccggcg cagtgatctt
     6541 aatagcagct gcgggtggcg gttattttta ctaccagcac tatcaagaaa cccaagcagt
     6601 agaagctgga gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg gagattataa
     6661 caaagctgca gaaatgacat caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga
     6721 gatcttagat aaataccaga atatatacgg tgctgctgat gtcaaaggac ttcagatatc
     6781 gaatctaaaa gtagataaaa aagacgattc tacttacagt ttttcatata aagcaaagat
     6841 gaatacctca ttaggtgaat tgaaagatct ttcttataaa ggaacattag acagaaatga
     6901 tggtcaaacc acgatcaact ggcaacccaa cttggttttt ccagaaatgg aaggaaatga
     6961 caaagtaagc ctgaccacac aagaagcagc aagagggaat attatagatc gaaatggtga
     7021 accattagca acaacaggca aactaaaaca attaggagtc gttccaagta aacttggaga
     7081 tggaggcgaa aaaacagcca atatcaaagc gattgcttcc tccttcgact taacagaaga
     7141 tgctatcaat caggcgattt cacaaagctg ggtacaaccc gattactttg tcccattgaa
     7201 aatcattgat ggagcaacgc cagaacttcc agctggagct accattcaag aagtagacgg
     7261 cagatattat cctttgggtg aagcagctgc tcagctgatt ggttacgtgg gagatatcac
     7321 agcagaagat attgataaga atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg
     7381 tttggagatg gcttttgata aggatcttcg tgggacaaca ggtgggaaat taagcattac
     7441 agatgcagac ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa atggcaaaga
     7501 tatcaaattg acaatcgatg cgaaggcaca aaaaatagct tttgacagtc taggaggaaa
     7561 agctgcatca actgttgcga caacgccaaa aaccggtgat cttcttgcgc ttgctagctc
     7621 tccaagctat gatccaaaca aaatgacaaa cgggatctca caagaagatt acaaagctta
     7681 tgaagaaaat cctgaacatc cattcatcag ccgatttgcg acaggttatg ctcctggatc
     7741 tacgtttaaa atgattacag cagcaatcgg tctcgacaac ggcactatcg atccaaatga
     7801 agtgttgacg atcaacgggc ttaaatggca aaaagacagt tcttggggat cttttaaaac
     7861 aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa actgctttga tctattccga
     7921 taatatatat gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt
     7981 agataaattc atttttggtg aagaccttga tttgccaatt agtatgaatc cagcacaaat
     8041 ttctaatgaa gatagcttta actcagatat cttgctagct gatactggat atggacaggg
     8101 cgaacttcta attactccta tccagcaagc agcaatgtat tctgttttta ccaacaatgg
     8161 cacacttgtc tatcctaaat tgattgcaga taaagagaca aaagataaga agaatgtcat
     8221 cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa
     8281 tggtacagca cattctcttt ctgctttagg gattccgttg gcagcgaaaa ctggtacagc
     8341 ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa
     8401 tcttgataac caaggttata tgatggtcag catgttggaa aataaagaag atgatgattc
     8461 agcaaccaaa cgagcatcgg aactattgca atacctcaac caaaattatc aataaaaaat
     8521 tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc acagtccttt tttcaaataa
     8581 acggttttca cgatgtctct ttcacaactt ctttttgatt tactcacact tcataaactt
     8641 tttttgtaaa atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa
     8701 ctaaaggtga atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga
     8761 tcaaagccat ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag
     8821 aaaaaccggc aatctgagca ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa
     8881 gcgtcaatat agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc
     8941 ctacactgac aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt
     9001 atacttcttc tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga
     9061 cagagctaag cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata
     9121 cgatgataat agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa
     9181 tccatttgac taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg
     9241 gtaaagaagc attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta
     9301 aaacgactaa aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat
     9361 tcactacgtt tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa
     9421 taatcccaaa gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc
     9481 caaaaacaac cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt
     9541 ttttcaaaag agataaatca ctttccagac cagctaaaaa cattaataag atcacaccaa
     9601 tctccgaaaa gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta
     9661 gcaaaacacc aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat
     9721 gtgagccaat cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca
     9781 tcattccttt gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag
     9841 gaaaatatac tcacaaaaaa accaacaatc ca
//
LOCUS       DARDHD010000100         9855 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000100 DARDHD010000000
VERSION     DARDHD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.100"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..405)
                     /locus_tag="SDA06_001886"
     CDS             complement(<1..405)
                     /locus_tag="SDA06_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4229153.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQ"
     gene            complement(395..2569)
                     /locus_tag="SDA06_001887"
     CDS             complement(395..2569)
                     /locus_tag="SDA06_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG4229154.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2585..4927)
                     /locus_tag="SDA06_001888"
     CDS             complement(2585..4927)
                     /locus_tag="SDA06_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229155.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4949..7492)
                     /locus_tag="SDA06_001889"
     CDS             complement(4949..7492)
                     /locus_tag="SDA06_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4229156.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7502..7900)
                     /locus_tag="SDA06_001890"
     CDS             complement(7502..7900)
                     /locus_tag="SDA06_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229157.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7981..8265)
                     /locus_tag="SDA06_001891"
     CDS             complement(7981..8265)
                     /locus_tag="SDA06_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229158.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8289..9287)
                     /locus_tag="SDA06_001892"
     CDS             complement(8289..9287)
                     /locus_tag="SDA06_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229159.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            9426..>9855
                     /locus_tag="SDA06_001893"
     CDS             9426..>9855
                     /locus_tag="SDA06_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4229160.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         2852 a         2070 c         1561 g         3372 t
ORIGIN      
        1 ttgattcaat gtcttttcaa aaaagaatgt ttttctctca atttttttga cgtttctatc
       61 atgaatcacg agaaaacttg ggacactttt aacatggtaa cgttgtaaaa aagtttgaag
      121 ttcttcttgt cttggttctt ttcttgtttc aattcgatat acattcctag agaatttttt
      181 gtcgtatctt tccaagaaag attcaacttc ttggcatttt ttacaatcat ttcttgctag
      241 atacacaaaa aatgattgat tcgttttttg aagttctagt acttcttcta aattactatt
      301 gattagtttt ccatttgatt cttcgttagt ttttatcagt ttaaaacaac caaataagag
      361 gaaactaaca ataagtatcc taacaataac tttcttattt cgcatattgt ttctccaaat
      421 ttggtttccg aatatagacg actcgcggtg gttcctggac gtcttcagct gttcgataca
      481 ccacaaattc gcctgttctc aatgctcgaa tgcgattcgg attgaattta aagcggtcta
      541 cgtctcgttt cgtgcctctt tctcctgtcc aatcaacctt tcccatatca gaataacctg
      601 cttcttgtgt cgtcatttcc gttaattcaa tgtcttcttc tgtccccatt aaatctgccc
      661 aataatcaat ttctgtcggg tcatttgtct tcccaataaa gaacgtattc acattcccaa
      721 tcaactgttt tcgaaggaca ggatcaattc tatcaatgtc cgcaagcgtt tgaggggcat
      781 acgccgcatg caccccagcg cctcgtccct tattgactaa gtctataagg ttatcattta
      841 gataagaagc tggttcgtca aatattacta ggactttcga gcgattggct gtttctcctg
      901 cttgtttata cacttctgac gcaaagaaat taatatctcc cacaagcatt tgagccaatg
      961 tccgaataaa ctcactataa atcaatccgt ttaacgagat ataaacaatc tttccttctt
     1021 taacaacttc ttgaatatct aattctcttt ttcctccaga agtatcaaag agttcaccaa
     1081 gctcactatc caataacata ttgatattgg tctgtaaatt ctcagaagtc gcatacaaat
     1141 aaaacgggtt cttcttatgt tcgtaacgaa caaacaaccg tttaaacaac gtttgctcga
     1201 tttctgtcat gagagaaatg ttttctttta ataatagata atacgtttct aagtcaacgg
     1261 ttttacgatc atgttcaacg attcgctctt cacttaactg ttccacatcg gcaaaatcac
     1321 gttctgtttt gtccatcgct tcattctttt tcgttttttt cttcgtggtt ttcttctttt
     1381 ctacgaccac tttttcttta aataaatctg gatttttcaa ctgaatacga tccgcaaaca
     1441 aatgtaagac gtttcgtggc aataaataat gttgtaacca aactagattt ctttcaattt
     1501 tatggctctt ttggaactcg tctaaaagct gaatcgttac ctgtaataac gctttggctg
     1561 ctgtcgaata atacacgctt tctgtttctg ctagattggt taatttatct cgaatggcaa
     1621 tatcattgcc attttttaca ggattataac gcaaatcgcc accgtccaca aagacttcaa
     1681 tcgggcgatt gtatgcctct gccacttctt ttaacgcttg taaagtctct cctgctcctt
     1741 tcccatcgac taagataacg gggatacgaa acttacacgc atgttctgct agtaattgga
     1801 tcaccgtcgt ttttcctgaa cctgtagtcg ctggaataat cacatgttgg ttcaattcac
     1861 taaagttcat cacataaggg agcccatact catctgttcc taatagaatc actttggcta
     1921 atgaagccag ttgtttattc cgcaaagcaa cgtcttttat gacaattgct acctggtacg
     1981 cttgctgata ctctgctgta tatttttggg actcctgaaa tttattcttc cgacgttcct
     2041 tgtattcttt actatttagc aaagctgttt gccgttcttt ctttgaaaca acttttcttt
     2101 tttcaataaa agacgcatac gcaatcaaac aacttcctgc acctgtccca cctaaaagga
     2161 gggcaaaaat cgtttgtcca ttaaactgca acgcatgaga aaacaagttt aatggaaata
     2221 ataagtaaca cgacaaatac cctctaggaa gaacaaaaat aaaacaaaat agatttaata
     2281 gcgtaaaacc taacgcaaaa ataaaaaaaa gagagcttct tcgttttaag aagctctcat
     2341 actcaaagtc ataaatatgt gcacgtgtct cttttttatc tatcataaag acaatcgcta
     2401 aaggaatact gcctacaata aaaaatggat atagacaaac acctaataaa gtgacgataa
     2461 aataaagttg ttgaaaccga ctttttggcg tttctttttc ttgttttctt ttgcgatcga
     2521 cagaaaacat ttgctcaaaa tgtgtcggtc gttttggtgc attcatcata ctatcgattt
     2581 cctcctatcg ttttcgttgt ttcatttctc ttcgataaat cggttgtttt tctcgttttc
     2641 gtttcatttt tgcttgagtt gctcgaaact cactccgtgt cttcggtgct tgttttggtg
     2701 attttgtttg tttcatggtt gagtatgctt tagatacggt tgaaggcgct ttggtttgtg
     2761 ctgttactcg attgactgct gacatatgtt cccgaacccc ctctggacgt cgaacagatt
     2821 tcggtgtggt agaacctgtt gctcgtggta cattcccttt tcttagtggt ttttgtaccg
     2881 catgtttttg tcctgcaact gtccgggttt tcatcgaact tgtttttgtt acgggagtaa
     2941 gtgcctcctt ccgacgacga cttgtttgtc cctgacgacg ttttcgatcg ttcaattctg
     3001 ttctaggcgt atgcttctgg ccataagact tgtgtttttg ctttggattc gctaatgaca
     3061 ccaccattgc agaaccctga ttgcgatacg gactacgaga cgcacctttt ctacttcttt
     3121 ctcttagact aggattgcct tgcaattctc gttgtaataa atcggctgtt tttcttgcct
     3181 gtttctccat tgctgttcgt tgcatacgtt cttgactttg ctcttctgct tgtttcgctt
     3241 gatcatgcaa gtctcttgct ttttgtgcca tacgttccat acgatttggt gctttatgac
     3301 gaggttgttg tgcggattca cgccgaaaag cacttcgtgt ggcttgtttc gccgtttgtg
     3361 gcatacgaac gtctgagcca cccccacgtt ttcgttgacg ttcttgtctc ttccgtgcgt
     3421 tttctttttc tttttgtttc tgtgcttttc tcgcttcttt gcgttctttc aaccgttgtt
     3481 tggcttccct tctcaatttt gcttctgccc caaatggatt tcccgcaaaa gctaacgctc
     3541 ctcgaacacc cattcttcca gtaaatagac tgcctaaaaa gcctcggaac aaataaattc
     3601 ccaacggcgc aaacaaataa acaatgatca agagaatttt ttcccatgga tcgtcttgtg
     3661 tactacttaa ttggaacccg taactaagaa aagaagaaaa aaggatacat gcaaaacctg
     3721 tcattccttt taaaaacgtc aaaataccaa aaactttggc atagttacca aacacattca
     3781 ttgtgacaag aaacaatcct aaaaaaagta agaacggcaa catcggtact aacaatagtt
     3841 gcatagttcc aataatcaat cgaaacatac ttaagacaaa ataaacaacg gtttgaatca
     3901 gattcacgac aaaataaaac aaggcaaatg ctgtattttt ccaactattg taatattgta
     3961 tgttcttgtt tttcagtgta tctgcttcat actctgtgac tttgtcatga attttctccc
     4021 ctatttttgt acctaaatca ttgtccaata aaagattgat tcgatcatat tcttttccag
     4081 catatttgac tttcttctca cgaattgtgt ctacattggt tgtgccataa tttaacaaca
     4141 gatatggttc ataaacatta ctataaaaga cccgagccgc catcaatttg cctgccgttt
     4201 ccactttttt atccgctgtt acctcttctg tttcttttgt ttcataattt gtttcttctt
     4261 caggtgttaa cactggattg accttcacga acgccgtttc cactgtagaa tctaaagaaa
     4321 aaagaacatc aaaaagattc ttatttgtcc cagaatcttt taatacgctt aacccagtaa
     4381 aaataaggat cgtcataaga aagacacgga gtgcttgttt cattcgagcc tctccagcat
     4441 atttgattgc catcataatt gccgaaaaac tgattccaat agttcctgca atacttaaca
     4501 tattgtccgc aattttttgt gttaagtttt gaatgggttc tcgaatggcg gaaataatat
     4561 ctaattcaaa gagaaattca accattgaag cattgaattc tcctaacatt tttacgccac
     4621 tccaaacaaa atttttcatc actcctgcaa cattgtacga tagctttttt ttaaggttca
     4681 ttgactcata gtcattggtc atcaattcaa acgaattctt tttgtaattt ttatacaatt
     4741 gatccacatc agtcgttact tcttttactt ctactttatc gttggcattc tctccttcag
     4801 aacgatcggt ttcagtgaca cccgcaccat cattcatttg atcggcagaa acagtagccc
     4861 cttgtagaca gaaaaaaaga ccccctaata aaaggagcgt ccaccatttc agagattttt
     4921 ttcgcaaaaa aaccacctct tttttgtttt atcgttgttt ttcattttct aacattgctt
     4981 gaattttttc atcttgattt tcagtagaag aaacagccaa taaccaacta tcaaacaaca
     5041 catcaatggc aatcggttga tttcttccca aatgatcctg gaataaacag gttcctgttt
     5101 gtaattgttc catcatttct aaattggctg aattcacttc catatcaaag aaggacaata
     5161 gtttctctcg tgcttctttt tgtttcggac gaaacgcaaa tttgtaagaa attaactctt
     5221 ttttatcttc ttgatcgtaa tctttaaacg cttgcacaac aaggtaaata tccgtattat
     5281 tcgcacgtcc ttttcgcaag ctatcatcaa tcaaatagct tccttgtgcg gtatcttcat
     5341 acccttttgc ctcgtcataa ataatcgcag cgtcttcttc tttgtttgta gaaaagatat
     5401 acgtaaactt cataatgact tccataattg ccattcctgc aagttgttcg ctatttaatc
     5461 gacccgaatc aatctctttt tgtgtcggaa tgcgtattcc ttgtgtccct aacacgttga
     5521 tttgtgaatc aaaacgtatc ggcgtactgt gctcattccc aatgagaatc tttcctaaac
     5581 caacgctatg ccctaaaatc aatttagcta gttctttgtc actttttcga atttcttcga
     5641 taacttgact taacgaatgc ttacctggca tggtgtaaac tacatcgatg gcgtcttgga
     5701 tcaccgtttt cttgtggctc gctgtttctc gattggtatc tacttctcct aacattccta
     5761 acacattttt agcggtttga attgcctctt cttttggtaa aaacaataga ggatcaagca
     5821 tccctcgaca gctttcatct gaagataacg aaataaaatt aattcgttca tacatagcca
     5881 caaactctgg tatgtgtccg aatttttcaa gtgcctttaa aaagaacttt tgggtttcat
     5941 ttttcggatc aatataaaga attttttgac ctaaaaacat cagccataaa aaaatatatt
     6001 ttacaaggac cgattttcct ttacctggtg gaccaataat caacgtattt ccattggtat
     6061 gttccgcgcc ttcaatactt ttctttgtta aattgggata ataccatact agtttactag
     6121 atagcgctaa cgcttctttc gcagatttaa acttttttgc aataacaatc cgtcctaaag
     6181 gaaatccttt cttatttcct acttctttct ttaagtccaa gccataatct gccacaaaac
     6241 ctgtcgtagc gacctgctcg taactacgaa aacgttgcgt acttccaatt aagcattgat
     6301 taaacaacgt tagctgatcc gctaccgatc gataaaattt gaaatctgta gactcaatcg
     6361 caaattcaat atctttgact cgttcttcca attcttcttt tgaagaagca gatacaacca
     6421 gcgtgacggt agaataacac aatcgttttt ctttgcgctt cagcttatct tttacatcca
     6481 ttaaccgttc tgtaccaaaa tttaatacat cgtctgaatc taaaatgccg tcaacggtct
     6541 catattcttt ttcttgttca taaatccgtc cagccatttt attaattttt cgttcatcag
     6601 attttgaatg ctcaaatcga agtcgaatat gcgtatcaat tggaaaagat aagctatcct
     6661 gaagattttg aataaagcta ctcccataca tggctaaagg agcgtccgtt tgacttaaaa
     6721 acgtcgaata atgcgtttga ttcaactgtt caatcgcaat gtatcctgct tcattcgaaa
     6781 tgacgccctc gcaaatttcc attggcgtaa gctgtctttc aggcaatttt tcttctgatc
     6841 gatggaaaaa ataataataa atttgtccaa tctcttgggc agataagcgc tctatcggct
     6901 tataattgga caattccgta tagagctcct tttctaatgt ttcatacgtt ggtaacacac
     6961 tacttatagg aactcgttgt cccgtaatac gatagataaa ctttttaaaa acatgcttaa
     7021 gcaattgaaa atattcgatc ggatcagaca ctttcatttc tgtagtgaaa cgcacaaaca
     7081 gatacgttcg atactcgttt aattgcactt cgtcttttaa aatctttcct gcctgttcaa
     7141 agtacacttt cccaagatct gcgcattctc cctttaccaa atctcgaatg gtttcttcaa
     7201 tgtgcgtatc taggttaaat tgacgtggaa tattgataaa atgatagtca tattcatcat
     7261 gtccaagtat gccttcgcca tcttgaagat agttcttgaa aaaatcaatg tcatttaaag
     7321 gaaatacttg ggaagaaagt tgatagccta cccacacatc atcatttttg gtcaaaacaa
     7381 aatttcggat aatggcgtct atcggaaatt ctaatcgagg aagcttcgtt tttttctttt
     7441 tgaactgcca aatcccgata ataaagacga ccaaaagaaa caaaaacgcc atttactcga
     7501 ctcactcctt ccatttgtgt agggaatacg tcactttttc atggaaaaac ggcacataca
     7561 tttcatgttc aaagcgttgt ttccgtttct gataatagga cgttcgccca attaaatact
     7621 cgaaaaagtg tttattgtcc catttcattg aaaacaatgt ccaaacgaga acaccaatcc
     7681 caaaaatcat aatcaaccag gactttcgaa acagtgtctg caagaaacta atttgattca
     7741 catacgcaat caaaaatagg atcaaaagaa tgaagagaaa taccccacct atgactaatt
     7801 gttccatgcg aatgccatta gaaatccagg gaattttttg tcctttaatc ccgtaaattt
     7861 tgtgagggtg cttaaactcc tttgtatagt tgtattccat gttcactcta cctcctttta
     7921 caaaaaaaga agcgaccaga agttctaatc ttctgatcgc ttttgcagtt tgtctttttc
     7981 ttatgataat tgtatccagg gcacaatcat ttgcgttacc cccacgttct cttcactatc
     8041 cttaataaag ataatgttct ttagccattc accaatatca acggcattcg ataacaataa
     8101 gacgagcatt atcaaaataa agacgccaat aatggcaaac gctgcccgcg caaagccttg
     8161 ggtcgcataa gcaccaacca ttagaacaat cgcaacgatt gccaatagcc aaatgacttg
     8221 tccagataca tcatcaaata acgatttaag atcaaaaagt ttcatttcca ttctcctcta
     8281 agttcatttt attcatttaa cgtagtaaca aaataactat cgccacttgt ttttacttcc
     8341 attgaaaagt ttgattggaa ctcgtttcct ttctcaaaat agaaacgata cttcccttgc
     8401 aaaaagtagt ttccttcttt attttttact gcatgatcca gttgtacatt gatcaattcg
     8461 ccgccttcta accctttttc gttagaaatc aatgccaatt tttcatcatt tttgacatat
     8521 aactcaaaaa accgtttcac aaatgcttcc agcttgattt cttcgctttc agtgaccgct
     8581 tgcccagaag aataaaatcg attttcctga tagttcacag aattggtatt cttactctca
     8641 gaaagattca aaaattgaac gccatcaagt acttttaaaa cattgttttg ataactgact
     8701 ggaaaagaaa gttgactgct tttccaatgt tctccttctg taaaagtaac ctcataaaat
     8761 aggcgataca ttgcttgtgt caaatcgatt tcttctagtt taataaagcg aacatctttc
     8821 gctactcgat tggtagccgt cgttacaaag cctagatcag acgcttccaa tccttgtgct
     8881 aaataatgat tgacggaagc ctcccattcg tttcgttttt ctggcgaata agtaaataac
     8941 ctttcaataa aatgttgtcc ctcgtacttc aattgttccg tagaagacaa ggtttggttg
     9001 acttcagtta acaatgtttc tggattgact tgttttttca ctgctaaacg cgttaatgta
     9061 tccactcgtc caaagtttag gaggagcacg acacccatta acagaaacaa acaaaaaaaa
     9121 gcgcctgaaa atacactcat ggcacttttt tgcgaaaatt ggatcattcg atctgacggt
     9181 tctgttttct ttttttggaa caaaaaacgt ttcttttgtt tcttttcctt ctgttgtttc
     9241 ggtggcttag tttggaacgc ttgtggatta aattttgcat tactcatgag aaatgccata
     9301 ccgttctaac aataatttcg gtattttgtc cacaatcact acttcgtgga caaccacact
     9361 tgccccctcc aatgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc
     9421 tttttttgcg gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata
     9481 acgaaggcgg ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac
     9541 attgggtaag gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct
     9601 acactacaaa atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc
     9661 aagaaactgc taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc
     9721 aatatgaaga aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta
     9781 tgaattccaa gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta
     9841 ttcgtttaag agcag
//
LOCUS       DARDHD010000101         9823 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000101 DARDHD010000000
VERSION     DARDHD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.101"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(31..2151)
                     /locus_tag="SDA06_001894"
     CDS             complement(31..2151)
                     /locus_tag="SDA06_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4229161.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2381..3559
                     /locus_tag="SDA06_001895"
     CDS             2381..3559
                     /locus_tag="SDA06_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG4229162.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3575..4333
                     /gene="aroD"
                     /locus_tag="SDA06_001896"
     CDS             3575..4333
                     /gene="aroD"
                     /locus_tag="SDA06_001896"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG4229163.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4356..4841
                     /gene="ybaK"
                     /locus_tag="SDA06_001897"
     CDS             4356..4841
                     /gene="ybaK"
                     /locus_tag="SDA06_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG4229164.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4912..6165)
                     /locus_tag="SDA06_001898"
     CDS             complement(4912..6165)
                     /locus_tag="SDA06_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4229165.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6283..7632
                     /gene="gdhA"
                     /locus_tag="SDA06_001899"
     CDS             6283..7632
                     /gene="gdhA"
                     /locus_tag="SDA06_001899"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG4229166.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7744..9090
                     /locus_tag="SDA06_001900"
     CDS             7744..9090
                     /locus_tag="SDA06_001900"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG4229167.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9398..9784)
                     /locus_tag="SDA06_001901"
     CDS             complement(9398..9784)
                     /locus_tag="SDA06_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4229168.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1784 c         2016 g         2895 t
ORIGIN      
        1 tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg tcgttccgtg
       61 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      121 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      181 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      241 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      301 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
      361 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
      421 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
      481 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
      541 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
      601 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
      661 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
      721 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
      781 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
      841 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
      901 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
      961 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1021 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1081 atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1141 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1201 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1261 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1321 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     1381 gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata
     1441 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     1501 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     1561 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     1621 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     1681 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     1741 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     1801 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     1861 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     1921 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     1981 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2041 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2101 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2161 cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag ctcacttatt
     2221 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2281 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     2341 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     2401 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     2461 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     2521 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     2581 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     2641 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacggaat
     2701 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     2761 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcgt
     2821 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     2881 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     2941 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3001 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3061 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3121 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3181 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3241 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3301 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     3361 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     3421 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     3481 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     3541 aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg gattgaatat
     3601 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     3661 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     3721 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     3781 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     3841 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     3901 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     3961 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4021 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4081 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4141 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4201 tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4261 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4321 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     4381 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     4441 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     4501 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     4561 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     4621 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     4681 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     4741 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     4801 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     4861 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     4921 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     4981 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5041 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5101 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5161 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5221 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5281 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     5341 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     5401 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     5461 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     5521 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     5581 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     5641 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     5701 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     5761 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     5821 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     5881 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     5941 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6001 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6061 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6121 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6181 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa
     6241 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
     6301 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
     6361 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
     6421 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
     6481 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
     6541 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
     6601 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
     6661 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
     6721 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
     6781 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
     6841 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
     6901 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
     6961 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
     7021 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
     7081 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
     7141 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
     7201 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
     7261 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
     7321 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
     7381 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
     7441 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
     7501 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
     7561 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
     7621 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
     7681 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
     7741 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
     7801 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
     7861 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
     7921 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
     7981 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
     8041 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
     8101 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
     8161 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
     8221 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
     8281 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
     8341 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
     8401 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
     8461 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
     8521 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
     8581 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
     8641 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
     8701 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
     8761 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
     8821 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
     8881 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
     8941 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
     9001 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
     9061 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
     9121 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
     9181 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
     9241 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
     9301 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
     9361 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
     9421 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
     9481 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
     9541 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
     9601 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
     9661 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
     9721 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
     9781 tcatgaaaga tctccttttt tatttttatt atacccgtgg tgc
//
LOCUS       DARDHD010000102         9808 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000102 DARDHD010000000
VERSION     DARDHD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.102"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            201..680
                     /locus_tag="SDA06_001902"
     CDS             201..680
                     /locus_tag="SDA06_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229169.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="SDA06_001903"
     CDS             713..2941
                     /locus_tag="SDA06_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229170.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="SDA06_001904"
     CDS             3070..4713
                     /locus_tag="SDA06_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4229171.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="SDA06_001905"
     CDS             complement(4786..5331)
                     /locus_tag="SDA06_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG4229172.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="SDA06_001906"
     CDS             complement(5354..5839)
                     /locus_tag="SDA06_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4229173.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="SDA06_001907"
     CDS             complement(5829..6641)
                     /locus_tag="SDA06_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG4229174.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="SDA06_001908"
     CDS             complement(6780..8312)
                     /locus_tag="SDA06_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG4229175.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="SDA06_001909"
     CDS             complement(8421..9452)
                     /locus_tag="SDA06_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4229176.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3260 a         1827 c         1890 g         2831 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggata
//
LOCUS       DARDHD010000103         9703 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000103 DARDHD010000000
VERSION     DARDHD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.103"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(119..481)
                     /locus_tag="SDA06_001910"
     CDS             complement(119..481)
                     /locus_tag="SDA06_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229177.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(555..935)
                     /locus_tag="SDA06_001911"
     CDS             complement(555..935)
                     /locus_tag="SDA06_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG4229178.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            999..2192
                     /locus_tag="SDA06_001912"
     CDS             999..2192
                     /locus_tag="SDA06_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4229179.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2269..2853)
                     /locus_tag="SDA06_001913"
     CDS             complement(2269..2853)
                     /locus_tag="SDA06_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4229180.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3054..4052
                     /locus_tag="SDA06_001914"
     CDS             3054..4052
                     /locus_tag="SDA06_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4229181.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4164..4673
                     /locus_tag="SDA06_001915"
     CDS             4164..4673
                     /locus_tag="SDA06_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG4229182.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4703..5362)
                     /locus_tag="SDA06_001916"
     CDS             complement(4703..5362)
                     /locus_tag="SDA06_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG4229183.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5364..6128)
                     /locus_tag="SDA06_001917"
     CDS             complement(5364..6128)
                     /locus_tag="SDA06_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG4229184.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6136..6813)
                     /locus_tag="SDA06_001918"
     CDS             complement(6136..6813)
                     /locus_tag="SDA06_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG4229185.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6850..8235)
                     /locus_tag="SDA06_001919"
     CDS             complement(6850..8235)
                     /locus_tag="SDA06_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4229186.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8445..8529)
                     /gene="ffs"
                     /locus_tag="SDA06_001920"
     ncRNA           complement(8445..8529)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SDA06_001920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8552..9067)
                     /gene="tadA"
                     /locus_tag="SDA06_001921"
     CDS             complement(8552..9067)
                     /gene="tadA"
                     /locus_tag="SDA06_001921"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG4229187.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9174..>9703
                     /locus_tag="SDA06_001922"
     CDS             9174..>9703
                     /locus_tag="SDA06_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEG4229188.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKI"
BASE COUNT         2905 a         1951 c         1662 g         3185 t
ORIGIN      
        1 aaataaaatg cactaaaaaa ctatctttag cagaacattt cattgtattt tttggaccaa
       61 acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa tctgtatttt
      121 agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt tgacctggtt
      181 cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca gtttcaaaga
      241 caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc aaactaatct
      301 tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct aaaccagtct
      361 ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa tggaccaaaa
      421 attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt ttgcttgcca
      481 aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc acattttcag
      541 aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa tgcttcttct
      601 atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt tttattcgtg
      661 aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt tcttaaagat
      721 aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac tggctgtccg
      781 acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga aacatgaata
      841 agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat ccctgtcact
      901 tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt cgcatttatc
      961 attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt cttaagatga
     1021 tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg gacgcaattg
     1081 ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg gacttcttgt
     1141 ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc tttggttatg
     1201 ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa caccatgctg
     1261 tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt ttagctgcag
     1321 cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca gtttatcctc
     1381 cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct ttgctagaaa
     1441 aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa gaaaacaatg
     1501 taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg agcaaagaag
     1561 aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt agtgacgaga
     1621 tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg attgatccta
     1681 atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat ctagctgcaa
     1741 tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt gaaaaacatt
     1801 tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc caagcggctt
     1861 atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa aatgttcaaa
     1921 cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac cctgaaggaa
     1981 cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa ttggagaaca
     2041 ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt ccttccggtc
     2101 atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa ggacttaatc
     2161 gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa aaggacgaga
     2221 ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact attctgaaat
     2281 ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac gttgtacatt
     2341 tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg tatgtcggaa
     2401 atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg ggaatccagc
     2461 accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa taaagaattg
     2521 gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa ggttgaacag
     2581 ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac ccattcctgt
     2641 tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc
     2701 atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg gtgctagttc
     2761 agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt ttggtccttt
     2821 ttcacttttt aaatctagtt gtgggaatgc catattgttt cctcctaata tttgcatact
     2881 tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt gttttctttt
     2941 atttttcatc actagataca ttctataact taccgtttcg atttcgaatt caaacatttt
     3001 tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt tttatggaaa
     3061 tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct ttttacgcag
     3121 gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt ggtcaattag
     3181 caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca ataaaagcaa
     3241 gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca ttccatttaa
     3301 aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact acaaataaag
     3361 gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc cagccaagaa
     3421 aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat ttcgtgaatg
     3481 atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat tctgctatcg
     3541 attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat cgtcgcccgg
     3601 aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc aacttgctta
     3661 ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat atccggttaa
     3721 aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc gtcaattatg
     3781 gattctcttc taatttagaa catctttctt cttggggact tgatagtacc agaaatgcca
     3841 ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct ggtgacatct
     3901 gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca cctactgcag
     3961 tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt cacagcacaa
     4021 gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc atttcaagtt
     4081 aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa tagagaaaga
     4141 aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa gaaaaggcaa
     4201 gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt gtcatgtttt
     4261 tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt gtcaacagcg
     4321 tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta gatatattag
     4381 cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac gaaggccttt
     4441 acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg atcggcttta
     4501 cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta aacagccacg
     4561 atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc gctgctgctg
     4621 ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa taattcaaca
     4681 aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt ttcaagctat
     4741 tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa aataagataa
     4801 agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta acgggatcga
     4861 ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag cgatcaaatc
     4921 ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga aacggacgaa
     4981 tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata ctcgggatca
     5041 aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca taaaagtgat
     5101 gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg aagggattat
     5161 tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct aaagaagtat
     5221 aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag ttgatcgtca
     5281 caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca tattccatcc
     5341 actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg tttcacatag
     5401 gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga taaaacgagc
     5461 aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt atctccaacc
     5521 atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat aatgatggct
     5581 tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa agagatgact
     5641 gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg gttcgttcca
     5701 atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc ataggtcaga
     5761 taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt aaatccggca
     5821 gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt cccttttcca
     5881 tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc tggagctaca
     5941 tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt agtcgtgtta
     6001 ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa tcgtttaccg
     6061 gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa atatccttta
     6121 tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt ttgttttttc
     6181 tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg actctgtttt
     6241 tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt tctcatcgat
     6301 atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt cttttttacc
     6361 gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt aaagatagtc
     6421 ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt ggaaaaatcc
     6481 ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc gggctagtac
     6541 atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat cggacacaag
     6601 ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca ctagctcgcc
     6661 tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg tcagttcttc
     6721 ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg ttttatgctc
     6781 aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct tttttattat
     6841 accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca cacttcggat
     6901 aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa aaaatggaac
     6961 aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga ttggcttgtt
     7021 cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga ccatctgccc
     7081 aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa agttacgatt
     7141 tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga catttaacaa
     7201 gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc cttttggaat
     7261 ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc gttctttcac
     7321 cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac tcagagaaaa
     7381 tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta gataactatt
     7441 tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg ctttggctga
     7501 atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga catactgccc
     7561 gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc tgaataaatg
     7621 atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa tttggaaatc
     7681 atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac gtaaatcttc
     7741 gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg gattataagt
     7801 aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt tcgttgtaat
     7861 gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga ataagttatc
     7921 caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt tggaatttcc
     7981 tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca tcagcaagat
     8041 attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa aatcacctag
     8101 atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac gttgatccaa
     8161 aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg tatgtaaaat
     8221 cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca taggcagctt
     8281 tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc aaattgtata
     8341 agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt tttacagtac
     8401 tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa tcagacacat
     8461 ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa gcccaccatt
     8521 gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac cagtcttttt
     8581 ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc atcccacatt
     8641 cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg tcctccaaaa
     8701 gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa taaacttctg
     8761 ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag gtaacaaaaa
     8821 gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg attgcaagca
     8881 tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct cttccaatca
     8941 cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt tctgctttct
     9001 ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca agatttgtat
     9061 ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat atgataaaaa
     9121 ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac aagatgaaaa
     9181 tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca gcatcagaag
     9241 cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa gattcattat
     9301 ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat caaaaaaaac
     9361 acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa agctttcgtt
     9421 tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg caagatacta
     9481 ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca gatgcgctct
     9541 tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt atttttctat
     9601 ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc cattcacctg
     9661 gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttt
//
LOCUS       DARDHD010000104         9603 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000104 DARDHD010000000
VERSION     DARDHD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.104"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            179..2107
                     /locus_tag="SDA06_001923"
     CDS             179..2107
                     /locus_tag="SDA06_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4229189.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="SDA06_001924"
     CDS             2245..2700
                     /locus_tag="SDA06_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG4229190.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="SDA06_001925"
     CDS             3170..3439
                     /locus_tag="SDA06_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229191.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="SDA06_001926"
     CDS             3839..4093
                     /locus_tag="SDA06_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG4229192.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SDA06_001927"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SDA06_001927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG4229193.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="SDA06_001928"
     CDS             complement(5266..7539)
                     /locus_tag="SDA06_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG4229194.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="SDA06_001929"
     CDS             complement(7532..8071)
                     /locus_tag="SDA06_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG4229195.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="SDA06_001930"
     CDS             complement(8207..8425)
                     /locus_tag="SDA06_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG4229196.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="SDA06_001931"
     CDS             complement(8422..8667)
                     /locus_tag="SDA06_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4229197.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="SDA06_001932"
     CDS             complement(8783..9511)
                     /locus_tag="SDA06_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG4229198.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2970 a         1864 c         1665 g         3104 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaa
//
LOCUS       DARDHD010000105         9551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000105 DARDHD010000000
VERSION     DARDHD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.105"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1135)
                     /locus_tag="SDA06_001933"
     CDS             complement(<1..1135)
                     /locus_tag="SDA06_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG4229199.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKG"
     gene            complement(2007..3638)
                     /locus_tag="SDA06_001934"
     CDS             complement(2007..3638)
                     /locus_tag="SDA06_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG4229200.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3652..5325)
                     /locus_tag="SDA06_001935"
     CDS             complement(3652..5325)
                     /locus_tag="SDA06_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG4229201.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5338..5655)
                     /locus_tag="SDA06_001936"
     CDS             complement(5338..5655)
                     /locus_tag="SDA06_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4229202.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5680..7080)
                     /gene="lacG"
                     /locus_tag="SDA06_001937"
     CDS             complement(5680..7080)
                     /gene="lacG"
                     /locus_tag="SDA06_001937"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG4229203.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7317..8102
                     /locus_tag="SDA06_001938"
     CDS             7317..8102
                     /locus_tag="SDA06_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4229204.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8105..8797
                     /locus_tag="SDA06_001939"
     CDS             8105..8797
                     /locus_tag="SDA06_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4229205.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9071..9442)
                     /locus_tag="SDA06_001940"
     CDS             complement(9071..9442)
                     /locus_tag="SDA06_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4229206.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2882 a         2094 c         1678 g         2897 t
ORIGIN      
        1 aacctttatg actgcgacct aggaattttt ggttgtaagt atcaaaagac gagactaaga
       61 aacgttccat cgactcttca ttttggaact gttccttgcg gtggctgtat ttcttgattt
      121 gtttattaaa ggattcaatc aagttcgtag aataaatact cttgcgtatg gccaagggga
      181 aatcatagaa agtgagcaag tgatcgttcg aaagaatcga ttcaaccact tttggatagc
      241 tggttttcca tttttccgca aaagtaccac gtgcttccaa cgccacctct ttagatgagg
      301 cttgatacac cattttaaaa tcatcacaga cttccttacg atcatcgaca cgcactttat
      361 gactgatatt acgggaaacg tgtacacaac aatgttgaaa acgagctttg ggatagaacc
      421 gactaatcgc tcctaccatc ccctttaagc catccgtgat gaacaggagg acatttttca
      481 aaccgcgctc ttgaaggtcc aataaaattt cctcccaaat cgtgatggat tcagtcggtg
      541 caatcgcata gctcaatact tcctttgatc cgtccgggcg aatgccaact gcgatatgaa
      601 tagcttcctt ggcgacggtt ttccgcttta acggaatata cgttgcgtcc atataaatag
      661 cagcataacg gtcatgaagc tcccgcccat taaacgccgt tacctcttct gtaaatgatt
      721 ttgttatatt ggacatggtt tggggcgtgt agtaatgccc atacattttc tcaatcaagt
      781 ctgcgatttc cgacatggta ataccttttc ggaagaggtg aatgacggtc tcctctaacg
      841 tgtcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcacca ttgcggtcgc
      901 gcggaatctg gagatgaagt tccccatact cggtcttgac cgtacggtca taggagccgt
      961 tacgagaatt acccgtgtta aaaccaatgc gatcatactt ttcgtaatct aggaaagccg
     1021 ttaactccgt tttgagaagc gtattgactg ctttctccaa atggacacgg aataattcat
     1081 tcaaatcgcc tttgttcgct agagtcttta gaatttctgt agtaaaatcg ttcataggga
     1141 agtcctctct tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt
     1201 tctatttaca caaaatattt tacactctcc attataacaa ttgggttaaa aagaaaatag
     1261 atagtattct acctcatatt tatttgaaag taatatataa aagacgttca aattcgaacg
     1321 cctctcgtta accaaatatt ggattcagaa tctagttttc atatataaac agagtatgat
     1381 taggtgcaaa aattgcactt ttattgcact tttattttta tttatagtgt tttactgttc
     1441 tgtatattgt tttaactccc ttgatagtat cgcattctgt tttatacact ttacgttgaa
     1501 aacagagtgg gaataaagag ccaacataat atggaggggg aatccctata aatcaacatt
     1561 cttcaatagc taaaatgctt gattttcatt aaaaataaga tggttcccta ccaaaagaga
     1621 ctataatata agttaatttg tttcattctt atcataaaaa gaagttctcg aaaaacgaac
     1681 tgttttccga gaactttttt ctatccatct aagtgctcaa atcatattca cgacggaatt
     1741 ctaaggttac cctttcttaa acaaacttta tgttatctgc acaacttttt gcaatttttt
     1801 cattgcttct tacgaaatcg ttgaactctc tttagcataa tacaaagtgt agatgaagtc
     1861 tggtgcttta ctcgttttgg taaaaatgac tggaaatcgt ttctggttat ttttggcaaa
     1921 ggcgaggtgc aggttcgatt cctgccttct acgctaacga tgtggctgaa gggaacctcc
     1981 gatcttgaat cgaaacgatc cgcagtctat tgtttcacca atgaaacaaa atcaacgttc
     2041 gtccaagcat ttcctgaagc cttgctataa accccaattt cacaaacacc tgttgtaatt
     2101 ggaatgtctt tgattgtaat ttttgtccat tcacttgttt taacaaccgc agtatttagt
     2161 tccgctccac cataattttt gatatataac tgattagtat caaactctcc cccagacatt
     2221 acataggcac tcaatgtgta agttcctttg cttatgccac taacattttg ataagctgat
     2281 gcctcgtatg cttgatcctg ccaaaaagtt agtttgtagt ccccaaaaaa agtgcccgct
     2341 tccgttttga tcgtaccctt agcggttcct tgttgaaacc aggtttgcca attggaagga
     2401 ctggtcgttg gtccatcgct ttcgaaactt gcatttttaa ctagatttat ttgcgcccct
     2461 gcttgtttaa acgcacgcaa gcttggcaag gcgtttccag caaaatcaaa catcgcttgg
     2521 ttgtcccatt cgtttcccat agtggattga acactgagat aattcatacc tgcttgtgtt
     2581 gcccaactca catttccgtt gtaccaaaga ggttcccaat agaagaagcc cgccccgcga
     2641 ttattcggta ctgcctgaat ccgatcggca agatccagca aatactctgc ttgtcctgtc
     2701 ggtgttggtt catatccccc cgcagctgct tcggcttcac caaaagcatt tggcatagta
     2761 tcggcattcg ctgttgtatg cccataagct gtttcaacta cgatcacttc tttaccatac
     2821 cgttggctga tcgcattcat attggtttga agttcattca ttgaaccgtg ccaatacgga
     2881 taatacgaca ctccaattaa atcaaatgac acacctcgac tcgtgatttc atcaaaccac
     2941 caagtaaatg ctcctgtatc gccaccatca gctaagtgga gcattactgg agttgtttta
     3001 ggttgagtgt ctcgaacagc ttggatacca gacttcaaaa aagcagccaa tcgatcaaat
     3061 tctcccccgc cttctcccca agacttgcca taaggccaga gcattccgct attcagttca
     3121 ttgccgatct gaaccatatc aggataaacg tccagctgct tcatttccga cagaacgttc
     3181 tttgtatagt cgtacaatgc cgtattcatt tcttcaaagg tcagaccttg ccatgcttta
     3241 ggaagattct gtttcccagg atcaacccaa aaatcactgt agtgaaaatc cagcagcacc
     3301 ttaagtccca gattttttgc tcgttttgca aggtcaatcg tcgtttccag atcatttgtc
     3361 cctgctccat aactttctcc attactatta taaggatcct gccacaaacg cagtcgaaca
     3421 tagttgacac cattattttt taaaatctct aaggcatctt ttgaaatatc gtcttcataa
     3481 tattttgcac cagatttttc catatctgac aggatcgaaa tatctgctcc gcgaataaat
     3541 tcgctggcag atgcttcaac agtttttgaa acagccccca aagcccctaa cacagctgca
     3601 aataaaacag ctttacgcaa ccaaattccc attttcatta tggctcctcc tttattgaaa
     3661 ttggttcaga ataaactgaa cagctttttc aggatctctc gttaatccga tatattctgc
     3721 tccttttgtc gcaaccagct tgattcctaa gcgatcggtg tcttctttga tatcatcata
     3781 gtaagaattg acttgcggtg ctaagaccac catatcaaaa tcctgcagca tttcgtaatg
     3841 agaaccataa gcacccgcca ttgcactgat cggtgctttc gattcagatg cacctttttt
     3901 taaagcatta gctaacatcg cacttgtccc tgcgccagca caaagtacca acacattgat
     3961 ttctttattt gcatcgctga taacttgact cgctgccaca ggtacttcct cacgtacatc
     4021 ggcagaagca ccttcctcgg ttgcgatcac ctctgctttt tgtgcttctt tctctactaa
     4081 gacagcatcg tatgctttgc aaaatggtag atagatcagg aagtcaacga cgattagaac
     4141 cgctgctaga acaaaagcta agatacccat gcccgttcct agaaccaaac cgatcggtgc
     4201 tggagttgcc caaggcaaaa cgtacataaa gctgttcatg cccaatacat cgacaaaaaa
     4261 cttgaacagg caaacgttta caacaggtgt taacaaaaat ggaataaaaa aataagggtt
     4321 caaaatcatg ggtgccgcaa atagcaatgg ttcgttgaca gcgaaactta ctggaataaa
     4381 ggaagctttc ccaactgcct tcaactgctt agacttagca aagagtagga agataaaagg
     4441 aacaaccaat gttgccccag ttcctcccat agttcctaca aaattcccta aacccaccgt
     4501 cagtacttta gaagcttgtt caccggaatt gaacaattgc agattggttt ccacattggc
     4561 atagataatt gccgcaattg ccggctccac gatcgacggt ccatgaacac cgacaaacca
     4621 aaacagcgcc atcgctcccc agataatagc gattcctaag tacccgtcag ctgctgtaaa
     4681 tagcggctgc agcaccgtaa tgattgcttc tgcaaatgag atttggaaga tttgtcgaat
     4741 gcctaaatca attaaaacgg ccgcaaatac tgaaaaagag aaaggaaaaa tatctcggaa
     4801 ggtttgcgaa atcgttccag gaacttcttt aggcatttta atggtaatat ctttcagcac
     4861 acaaaactta tataaattaa ctgttaaaaa tgcagcgaca aaggaagcca gtagtccctt
     4921 tgtccccata tattccgttg aaaatccacc ttcaatcgga gagacgctta acaacaggaa
     4981 acttaccaca gaggccagca tcacagaggt ggtgttgatt actttgtttt tcggtaagcg
     5041 acgattcatc gaatcagcca agcagcgagc agtggttgct gaaacgagta accctacaac
     5101 acccatcgaa taattataga ctttccatag ccatgtggag acagattcag gccattggta
     5161 accaaaaatc tcaggaaccg cagcaatcaa aatgaaaatg cttgaaaata aaatagctgg
     5221 catcgctgtt aaaaatccgt cacgaacggc tcctaaataa ctattacgtg agattttttc
     5281 aaaaaatggt ttcccttttt cgatttgttt aatgattcca tccatagcgt tcttctccta
     5341 tccttgattc tgtctgtaca gtacaataaa gtcttgaatc agatctctca gcaataaagt
     5401 ggtcattaaa tgatcctgcc catggataaa aatgaatccc atctccatct ctttcccaga
     5461 tgcttcttcc gctaaaattt ttgtttgcga attatgtgca agggttaaat tttcgtcggc
     5521 ttctttcaac gccgaatcaa catgttcaaa gtttccttgt cttacttctt tcaataattt
     5581 caataatgta ctgcgagcgt cgcctgaata tgcaacgatc tcaaaaccaa gcatttgtaa
     5641 ttcctcttta ttcatctttt cttcctccat tatcacttat taaataatcg ttttggtttc
     5701 actgaccaac ttgtaccagt aagctgattc ttttgggtaa cgcttctggg tttcaaaatc
     5761 aacataaaac aagccatacc ttttgttata cccatttgtc catgagaaaa gatccataag
     5821 cgaccacaaa aaatacccct tcacatttac cccggctgtt atcgccttgc ttaaggattc
     5881 caaatagact ctcaaatagt cgattctcgg ctgatccata ataatgccat cttcaaactg
     5941 atctttatac cccatcccgt tctcagtgat atagattttg ttgtaatgtg ggtaatcgct
     6001 tttaattcgc agcaacaagt catatagacc ttctggataa atcagccagt cccaatcagt
     6061 tcgaggaatt ccttctttat aaatacgttc tccgattccc ttcactttat aaacagaggt
     6121 ccctttttca cccgtaccgt tatgatggat tgcattttct ccatcgtaag ctttgacaaa
     6181 atggcattgg tagtgattga tccctaagta gtcattacgc gtggatgctt ttttcaattc
     6241 aacgaaatct tcttcaggaa aatgatagct ggcctgattt gcttcacata tctcatcaag
     6301 agcagtcaag gtttcagttg aataatagcc taaataggta gcatctaata agaaacggat
     6361 cgacaatgca tcgtctaaaa aggcagcatg cttatcttcc ggcgcgtctg ttgccgcata
     6421 tttcgtctct aatgagtgaa ctacaccaat ttcacccgga agttcgtttt ctttgaaata
     6481 gttcactaca cgggcatgag caaccatcat attatgaaga caggcgacga tctttgtaaa
     6541 atcatatttg attcctggtg ggaaaacacc taacaaatat tgattggttg caactggata
     6601 gatttcatta aaggtactcc acaccttgac ttctttgaat tcatgaaaac aaaaaatggc
     6661 ataggaaaca aatgcttcta ttgtttctcg attcaaaaaa tctccatggt caaacaaacg
     6721 tttaggggta tcaaaatgat gcaacgtcac aaacggcgtt acgtttcttt tcttacattc
     6781 ttcgaacaca cgatgataaa aagcgattcc ttctggattt ggtttcccag ctccatcagg
     6841 aaaaatgcgg ctccaagcaa tggataatct tagaccattt acaccaaaac gttcacacaa
     6901 ttcaatgtcc tttgcatatt gatgataaaa atcacttgcc ggatcgggac tgaatcgccc
     6961 ttgttcctct aaaaagtcat cccaagcaac agcgcctttg ccaccttcct tagtggctcc
     7021 ttcaacctga taggctgctg tggctccgcc aaaaataaaa tcttctggta atttaagcat
     7081 gtacatctct cctcaaatct ataatcagtt ttcgctcttt ctaactgatt gcattgtaaa
     7141 cgcatccaaa acatttgtca acaaaaacaa acacaaataa tcataaaaac cagaaaacag
     7201 caataaaatc tattgtaaca aacataaaaa ccaccctaaa tgtttacttt tgttcgaatt
     7261 agattgactg ttgtccgttt atatgataga ttatccttaa attagaggtg aagacaatgt
     7321 tgaaaaaaga aagattgctt actatcgtag aaatggtcaa taaaaaagga atccttaccg
     7381 ttaatgaaat catcaatcaa ttagatgttt ctgatatgac cgttcgcaga gatttagatg
     7441 aattagaaaa atcgggcaag ctgctgcgtg tccatggagg ggctcaaagt ctttcctaca
     7501 ctttagatca agaactttct cacactgaaa aagccactct gcagattcaa gaaaaaaaag
     7561 aaattgctgc cacagcagcc tccttgattc acgagggaga aacgattttc ttaggaccag
     7621 gtacaacaat cgaactattg gcgacgcatt tactgaacaa gaaaattcgg atcattacaa
     7681 ataattatcc agtttttgat attcttgctc aagcagatgg accagaaatt ctactgattg
     7741 gcggtgattt ccggaaaaat accggcgcat ttgtcggacc gatcaccaac gacaatttaa
     7801 gaagactaaa gtttacaaaa gcatttatca gttcaaatgg cgttcacaac gaagcgatct
     7861 ctacttacag taccgaagaa ggagaagcac agcagatcgc tctaaataat tcccgtaaga
     7921 aatatctact cgttgataat aaaaaattca atcgagatga tttttatgtg ttttataatt
     7981 tgcatgattt cgatctgctc attacggatc gccagatctc gcgggaggtt caagagcact
     8041 atgcacaata tgtcgatctg caaattgctg attcaaccga aaataccact ataggagcgt
     8101 aactatgaaa gggattatat ttgatttcaa cggaacaatg tttttggact ctcatctgca
     8161 tgaagctgct tggctgcata tgatacatga tcatacaaaa gatggtttat cagacgagga
     8221 tattttaaaa aatattcatg gtcggacgaa tacggagatt ttgaaacatt tcatttcaga
     8281 gcatttgacc gcctctgaga tcgccgtatt gtcagaagaa aaagaaagct attatcgtat
     8341 gctttgcttg caaaatgaaa acgagcttca gttgacaaaa gggcttgaag cgactcttga
     8401 ccagctgact gaccaacaga ttccattaac gattgccacg gctactgtca aagagaatgt
     8461 tgctttttat tttgatattt tcgatttagc ccgctggttt gactgggaca agattgtcta
     8521 tgacgacggc tctttccctg ggaaaccgca accggatatt tttcttaaag cggcagacaa
     8581 actgagctta gctccggctg attgtcttgt tatcgaagat gcttactcag gattacttgc
     8641 tgccaaacgt gcggggatcg gaacaattat tgcaattgat ccattcggta aaaacaaaac
     8701 agtatttgaa caggcacagc taaataaaga tggaaccatc aaagatttta ctggtttttg
     8761 gacagagcac ctgtctgcca catccgttaa ttcttaaacg attcaactaa ctaccaaaga
     8821 aaagcaactg aaatgcacac ctccttttat aaaaaactgc cagtgaaacg acatgaaaga
     8881 tacactgcta tatccttagt gaaatatagc agtgtatctt tgactttttc attcttttaa
     8941 ccatatgacg cgttcatctc tatctataat taacattaaa aatagtgttt tcacattatt
     9001 tctcaaacca atcttaaaaa tcatcaggag tacttttttg atatactttt attaatgctg
     9061 ggcgtaatgg ttaaaagatt tccactagtt cctctccacc aagctgagtt gaaaaatgct
     9121 tatctgtaaa caacatagac aaagaggaaa taaacgtttc cgtatctttc tctgtcccaa
     9181 ggatattaat taattttcct actaggtaag tatcattaaa cttaagatct gccttcacat
     9241 caaaagtttt gataaaagct tttatcatgc gtacaatttt gtctttatcg ccataattga
     9301 tttcaccact catatcaata tagtcactaa tccctgaaga aataatggca actgcttctt
     9361 ctttcgcctt atcagataat tcatttaatg actgggattg attgatgact tcttgagttt
     9421 tatctgttac gtaatctctc attatcctat cggcatactc cgcaaagtaa tcttgaaaaa
     9481 attcttctac agggtaacca acttcgtttt cccaaccagg agaaatttca ttatcagtac
     9541 ttattaaatc t
//
LOCUS       DARDHD010000106         9369 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000106 DARDHD010000000
VERSION     DARDHD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.106"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(89..1138)
                     /gene="recA"
                     /locus_tag="SDA06_001941"
     CDS             complement(89..1138)
                     /gene="recA"
                     /locus_tag="SDA06_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG4229207.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1247..2488)
                     /locus_tag="SDA06_001942"
     CDS             complement(1247..2488)
                     /locus_tag="SDA06_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG4229208.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2728..3306)
                     /gene="pgsA"
                     /locus_tag="SDA06_001943"
     CDS             complement(2728..3306)
                     /gene="pgsA"
                     /locus_tag="SDA06_001943"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG4229209.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3515..4438)
                     /locus_tag="SDA06_001944"
     CDS             complement(3515..4438)
                     /locus_tag="SDA06_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229210.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5177..6463)
                     /locus_tag="SDA06_001945"
     CDS             complement(5177..6463)
                     /locus_tag="SDA06_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4229211.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6456..7724)
                     /locus_tag="SDA06_001946"
     CDS             complement(6456..7724)
                     /locus_tag="SDA06_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4229212.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7976..8437
                     /gene="mscL"
                     /locus_tag="SDA06_001947"
     CDS             7976..8437
                     /gene="mscL"
                     /locus_tag="SDA06_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG4229213.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8497..8943)
                     /locus_tag="SDA06_001948"
     CDS             complement(8497..8943)
                     /locus_tag="SDA06_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229214.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9036..9281)
                     /locus_tag="SDA06_001949"
     CDS             complement(9036..9281)
                     /locus_tag="SDA06_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG4229215.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2600 a         2027 c         1659 g         3083 t
ORIGIN      
        1 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
       61 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
      121 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
      181 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
      241 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
      301 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
      361 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
      421 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
      481 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
      541 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
      601 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
      661 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
      721 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
      781 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
      841 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
      901 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
      961 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
     1021 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
     1081 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
     1141 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
     1201 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
     1261 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
     1321 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
     1381 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
     1441 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
     1501 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
     1561 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
     1621 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
     1681 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
     1741 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
     1801 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
     1861 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
     1921 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
     1981 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
     2041 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
     2101 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
     2161 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
     2221 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
     2281 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
     2341 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
     2401 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
     2461 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
     2521 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
     2581 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
     2641 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
     2701 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
     2761 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
     2821 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
     2881 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
     2941 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
     3001 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
     3061 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
     3121 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
     3181 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
     3241 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
     3301 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
     3361 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
     3421 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
     3481 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
     3541 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
     3601 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
     3661 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
     3721 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
     3781 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
     3841 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
     3901 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
     3961 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
     4021 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
     4081 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
     4141 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
     4201 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
     4261 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
     4321 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
     4381 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
     4441 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
     4501 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
     4561 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
     4621 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
     4681 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
     4741 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     4801 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
     4861 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
     4921 aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     4981 cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat aagttgatta
     5041 aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact aagaatacca
     5101 taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt aagacttttc
     5161 ctcataaaat actggtttat tttggtctca tgtaaaactc gctgaaggcc tcttcactta
     5221 taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg atttcaggaa
     5281 gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca atatattcga
     5341 tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa tcgagatttg
     5401 tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact ttttctgctg
     5461 ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca cgatccaagc
     5521 tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct agatagtttc
     5581 gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta tagcgtaaca
     5641 gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca aatttatctc
     5701 gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt tcagggaaat
     5761 aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc cgaataagtt
     5821 tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg cttggctgat
     5881 aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata ctttcaaccg
     5941 tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt atgccaaaaa
     6001 acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg attccttttt
     6061 ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca
     6121 gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta aaacttgtga
     6181 aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca tcttcttttt
     6241 caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc ttttcttttt
     6301 caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac aaaccatatg
     6361 tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc agtacttcat
     6421 gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt tcctcctgct
     6481 aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc tttgatcgtt
     6541 acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg cagccagctg
     6601 tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag atattggttt
     6661 ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt tcgcaaactt
     6721 tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc gataccggtt
     6781 tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata agctagactt
     6841 tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta
     6901 aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg ataagctagg
     6961 ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat attcacttga
     7021 ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg taattgccca
     7081 actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc gatttgatca
     7141 tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt gtccaaatct
     7201 tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata aagttcttgc
     7261 aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata tagacgcaga
     7321 ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat atttggataa
     7381 aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc attgacatat
     7441 tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt ttttctaact
     7501 gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt tgtctgatcc
     7561 gaataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact
     7621 tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta ttgttttgtt
     7681 ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat atgctccttt
     7741 tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt acttattcct
     7801 ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg ttacacttta
     7861 tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt
     7921 tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt cattcatgat
     7981 caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt
     8041 tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga tcacaccact
     8101 aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag cactttcaat
     8161 tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat
     8221 cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc
     8281 gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta ctgcagaaga
     8341 ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca atacacatcc
     8401 agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa aaaaaagaaa
     8461 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt gttccttttt
     8521 catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct tgaatccaaa
     8581 cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt tttctggaaa
     8641 atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac catctttacg
     8701 gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt catatactcg
     8761 cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag cttctccatc
     8821 aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg cgattcgttc
     8881 tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta cctgatattc
     8941 cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac acaaaaaagc
     9001 aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt taaaaattcg
     9061 atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc aaaagaattt
     9121 tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa aacgccgcgg
     9181 cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct gccgatacga
     9241 ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac ctctactatc
     9301 aactatacca catattttta aaattttcaa gtttaccata aaaaaaaaag aaatgcaata
     9361 aaaataaat
//
LOCUS       DARDHD010000107         9263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000107 DARDHD010000000
VERSION     DARDHD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.107"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..662)
                     /locus_tag="SDA06_001950"
     CDS             complement(<1..662)
                     /locus_tag="SDA06_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4229216.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QL"
     gene            796..1083
                     /locus_tag="SDA06_001951"
     CDS             796..1083
                     /locus_tag="SDA06_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229217.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1499..3037
                     /locus_tag="SDA06_001952"
     CDS             1499..3037
                     /locus_tag="SDA06_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG4229218.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3034..3999
                     /locus_tag="SDA06_001953"
     CDS             3034..3999
                     /locus_tag="SDA06_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229219.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3986..5308
                     /locus_tag="SDA06_001954"
     CDS             3986..5308
                     /locus_tag="SDA06_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4229220.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5371..5475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5549..6292
                     /locus_tag="SDA06_001955"
     CDS             5549..6292
                     /locus_tag="SDA06_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4229221.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6307..7299
                     /locus_tag="SDA06_001956"
     CDS             6307..7299
                     /locus_tag="SDA06_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4229222.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7311..8048
                     /locus_tag="SDA06_001957"
     CDS             7311..8048
                     /locus_tag="SDA06_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229223.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8038..8760
                     /locus_tag="SDA06_001958"
     CDS             8038..8760
                     /locus_tag="SDA06_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4229224.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8844..>9263)
                     /locus_tag="SDA06_001959"
     CDS             complement(8844..>9263)
                     /locus_tag="SDA06_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG4229225.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3027 a         1601 c         1960 g         2675 t
ORIGIN      
        1 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
       61 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      121 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      181 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      241 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      301 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      361 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      421 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      481 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      541 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      601 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      661 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      721 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      781 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      841 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
      901 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
      961 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
     1021 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
     1081 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
     1141 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
     1201 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
     1261 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
     1321 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
     1381 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
     1441 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
     1501 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
     1561 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
     1621 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
     1681 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
     1741 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
     1801 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
     1861 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
     1921 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
     1981 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
     2041 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
     2101 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
     2161 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
     2221 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
     2281 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
     2341 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
     2401 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
     2461 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
     2521 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
     2581 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
     2641 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
     2701 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
     2761 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
     2821 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
     2881 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acggaggatc aaatgattga
     2941 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
     3001 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
     3061 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
     3121 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
     3181 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
     3241 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
     3301 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
     3361 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
     3421 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
     3481 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
     3541 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
     3601 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
     3661 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
     3721 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
     3781 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
     3841 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
     3901 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
     3961 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
     4021 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
     4081 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
     4141 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
     4201 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
     4261 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
     4321 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
     4381 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
     4441 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
     4501 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
     4561 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
     4621 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
     4681 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
     4741 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
     4801 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
     4861 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
     4921 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
     4981 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
     5041 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
     5101 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
     5161 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
     5221 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
     5281 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
     5341 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
     5401 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
     5461 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
     5521 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
     5581 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
     5641 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
     5701 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
     5761 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
     5821 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
     5881 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
     5941 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
     6001 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
     6061 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
     6121 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
     6181 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
     6241 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
     6301 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
     6361 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
     6421 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
     6481 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
     6541 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
     6601 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
     6661 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
     6721 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
     6781 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
     6841 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
     6901 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
     6961 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
     7021 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
     7081 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
     7141 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
     7201 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
     7261 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
     7321 aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca
     7381 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
     7441 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
     7501 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
     7561 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
     7621 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
     7681 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
     7741 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
     7801 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
     7861 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
     7921 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
     7981 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
     8041 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
     8101 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
     8161 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
     8221 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
     8281 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
     8341 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
     8401 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
     8461 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
     8521 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
     8581 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
     8641 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
     8701 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
     8761 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
     8821 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc
     8881 gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca
     8941 gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg
     9001 ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt
     9061 gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca
     9121 ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc agcttggact
     9181 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     9241 attgttaatt gttgtcctga gta
//
LOCUS       DARDHD010000108         8868 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000108 DARDHD010000000
VERSION     DARDHD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.108"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..1294
                     /locus_tag="SDA06_001960"
                     /pseudo
     CDS             80..1294
                     /locus_tag="SDA06_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1473..1802)
                     /locus_tag="SDA06_001961"
     CDS             complement(1473..1802)
                     /locus_tag="SDA06_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4229226.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1792..2064)
                     /locus_tag="SDA06_001962"
     CDS             complement(1792..2064)
                     /locus_tag="SDA06_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4229227.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2515..3318)
                     /locus_tag="SDA06_001963"
     CDS             complement(2515..3318)
                     /locus_tag="SDA06_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4229228.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3296..4420)
                     /locus_tag="SDA06_001964"
     CDS             complement(3296..4420)
                     /locus_tag="SDA06_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229229.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4436..5251)
                     /locus_tag="SDA06_001965"
     CDS             complement(4436..5251)
                     /locus_tag="SDA06_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4229230.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5241..6113)
                     /locus_tag="SDA06_001966"
     CDS             complement(5241..6113)
                     /locus_tag="SDA06_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4229231.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6198..7487)
                     /locus_tag="SDA06_001967"
     CDS             complement(6198..7487)
                     /locus_tag="SDA06_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4229232.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7715..8359)
                     /locus_tag="SDA06_001968"
                     /pseudo
     CDS             complement(7715..8359)
                     /locus_tag="SDA06_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2752 a         1884 c         1412 g         2820 t
ORIGIN      
        1 acaaaatatt atagaagcaa aatcaattct accttagatg ttcttatatc actctatcag
       61 tcctatttga caaagagcca agaaaaagac gttggtcgtc gatgctgttc tctcgcctac
      121 tcctcgtgct tgtagaagtt gtggttctac tgtggtagat ggaaacggga aagcaattat
      181 agtgaaaaat gggaagaagg aaacgattgt ccgttttgaa caatacaatc atatgccttt
      241 ggttacgcgc ctaaaaaagc aacgctatac ctgtaaaaac tgtcgtaccc attggacggc
      301 tcaaagttat tttgtccgac tcagccattc aatcgcagat catgttcgat ataaaattgc
      361 ttctttactg actgaaaaag tatctttgtc ttttattgcg aaaagttgtc aggtatcttt
      421 gactaccgtt actcgtacat tgaaagagtt taaaagctat ttaccaaagc cttctaagaa
      481 gattctcccc agagtattga tggttgatga atttcgttcg cacgcttcca tagaagataa
      541 aatgagcttt atttgcgcag atggtgaaac aggaaactta attgctgtct tgcctacgcg
      601 taaattacct cgattaacaa gctatttctt aggctgcacc aatccagaag aagtagaatt
      661 cttagtcaca gacatgaacg ccgcttactt ccagcttacc aaacgtgttc tgccaaatgc
      721 gaaagtagtg attgatcggt ttcatattgt caaacacatg aatcaggcac tcaatgagtt
      781 gcgtatccgt gaaatgaatg agcttcgtaa agcaggacag aaaagtcagg cagaaaaact
      841 gaaaaagaac tggcgctttc tgctaaaaaa tcgtgcaagc atcaaccatt atgagtacaa
      901 aacatggaaa agtttccgag caccaaaata cccatttctc actgaagcaa tgatgattga
      961 tcgattgctt gagttttctg cgcctctaaa ggaggcgtat cccttttttc atgaattagt
     1021 cgaagccttt cgagacaaag accctgactt atttttctcc ttattggcag aacttcccga
     1081 aacgttagat gacggctttc gggagaagct tcaaaacctt ctgacctatg aagaaggtat
     1141 caccagcgca atgatctatc cttattccaa tggaaaaatt gaagcgaaga atacccacat
     1201 aaagacaatg aaacgagtat cctacggatt taaatcattc gagaacatga gaattagaat
     1261 ctttttgatc aatcaattaa tcaatgtaag ataacaaaaa atctgaacca gaatgtggtt
     1321 cattctgacc cggatttgat tttgcacaac tcatcagtcc ttattgacaa agagccaaaa
     1381 atctgagcca gaatgaggtt cattctgacc cagatttgat tttgtacaac ccatcagtcc
     1441 ttattgacaa agagcccttc tttttagtat tattataatt cttccatgcg ttcttccagt
     1501 cgctgtaaat cgcttggact taattcccca atttttttcc atttgacctt tttgttgttt
     1561 ttgggtaatt cgtttagagt tcctacgtca atccaagatt ctttttttag tcctgcctgt
     1621 ttccaatccc ttatttcaac gtatttcagc tttattttat cggatttagt atgatatttt
     1681 gaagtaattg acagaaacga aattgtgtcc agtccatttt ttagaatata gacaggccgg
     1741 cgtttaccgc cctctgcagc tgtgaaagca acgtagacgg ttgctatatc attagtattc
     1801 atcttcattg aaccattctt caattttttc gtcagtgtct agtttttgga caggcaattt
     1861 ttccgctaat ctttgaatat tatggattcg tttacttttt tccgacaatt caatagaaaa
     1921 tgggattttt ccttgctcaa caatttgctt ataaaaaacg gtgacggctg ttttggggct
     1981 catgcctagt tcatcaataa tgctttctgc ttcatttttc aatttttcgt ccatagaaat
     2041 ttgtaactta gccattttct ccacctcttt ctgacaaaca cattatatca cggaaagagt
     2101 tcaaatggaa ttaatacaac tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat
     2161 ataaggaaag gatatagtgc taaatggcct tgatagatag aaaagtttca aaatccgcca
     2221 cagcgataat tgaagcgaac actcaacgac acacagaaca ggaaaagact cctaaaagaa
     2281 ccaaattaac taggacgtgt ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa
     2341 cgaatggcac aaccacgaag accgatatgg ttcttggaaa actttctcca ccctttttgt
     2401 aattggaggg atagtggttt attgatagcg attttccaag ttcttcaagt ggaaccagac
     2461 attgagcacc acggctaaaa agtgaatgaa gtctttattt ttaatcaata atttttaatg
     2521 attaccagta attggttttt tcatgcgccc tatttctatt tgatgtggag ttaaataatg
     2581 ctgataagta aaaatttttc cgtcaattaa actgccacta gtgaatcctg ttctattggt
     2641 aaaaagagaa tagtctgggt attcatctac accaaatgat aaagattcag ccataaactg
     2701 ttttttacca gcaaattcag cactgctacc ttgatggata tgtcctacaa aaatacctaa
     2761 aatatcactt tcggcaatca attcagcaaa accttccgga acttcagtta caatagatgg
     2821 atcttcccac gacaatggat gatgaaccaa taaaatcgtt cccttttcag caggtgtacc
     2881 caaccactct tgtaattgaa tcatctgtcc tatcgatatt tttccttcgt gctgggtatc
     2941 ttgagcagaa tccaaggaaa taattcgtaa cccattttga ttcgtacaat ggatatactc
     3001 tcccgtttca ttgctttcaa cactcattcc ttcaaagaaa acactgcgac gatcatgatt
     3061 cccacgacaa aaatagtaag gtgtggttgg gaaatattga tccaacattt tccgtaagac
     3121 ttgataatca gaaatccccc cttcatgtac taaatcacct gttaatagaa taaaatcgta
     3181 cactgttaac gagagttctt gtaacaattt ttccaatgcc aactgtggat ttttaattgt
     3241 tggaaatagt tcagcagtaa acaagttagt ttgatagttt tctctaaaat ggatatcaga
     3301 gagatgcaaa aagcgcatgg ctattccctc tttcttcaat aaaataatca ggatatccta
     3361 aattttcttg cgtctgataa tcaaaaaaat gcattctttc aggttctatg cggagattga
     3421 ctgcatcacc aatctgccaa gtaggttttt cattaaggac aatcatttct ataccatcga
     3481 ctttaacggt gtacgcataa ctttgaccta atagctcacg atacttcact tgaccgtgta
     3541 aatcgcttgt ttcttttgaa aaggttacgt gttctggtct tatgcctaaa tacaatgagc
     3601 cactaacctg aagaagttgt ttttgccaag ctggattcaa tgttaaaaat tgactggcta
     3661 cctttaatgt tccttgatca taagtaactg gcataatatt catgcctggc gaaccaataa
     3721 aatttgcagt aaaagtattt ttaggtttcg tataaacatt tttcggcgta tcaatcatct
     3781 gaattctacc ttcattcaat aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat
     3841 catgagtgac gtagacaaaa gtttgtttat aacgttgatg aatttttacc aattcttttc
     3901 gtgcatccaa tcgtaaacgc acatccaaat ttgataacgg ttcatctaat aaaaaataat
     3961 tactccgttt aaccaccgca cgagccaatg ctacacgctg ccgttgccct cctgacaaat
     4021 ctttaggaag tcgatcttgg tatggcgtta aacctaacat cttcaaagct tcttccaaac
     4081 ggtgctgaat gtcagctgtc ttaattttat tcgcccttaa tgcaaaaata atattctcct
     4141 ttacagtcat gtgaggatat aaagcataat tttgaaaaac cattgcgatc tctcgttctc
     4201 cacaaggtag cttatttgct agttgtgtac ctaaaaaaag ctctccactc gtaatttttt
     4261 ccaaaccagc aatcattctc agcaaagtag attttcctga accagatggt cccagtaaca
     4321 caattcgttc tccagcggcc acagctaaat caatttcttt taaaatggta tgttctccat
     4381 atttttttcc gatatttctt aattcaagcg tttctcccat gatttgtcct ccaatttatt
     4441 taatacctgc agaaacaaaa gtcccgataa tatatttttg aaaaattgca tacaagacta
     4501 acggtacaaa cattgtaatt aaactaatcg ccattgctac ggtaaaattt gtcccacctt
     4561 cagaactaat ataggtctgc atcgccaacg gcaaagtata attatctact gtccgtaaaa
     4621 ttaataccgg ccaaacatac tcattccaag aggtaataaa aaaccaaata ccggtagaag
     4681 taatcgcatg tctagtaaga ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt
     4741 tatccaatgc cgccacatct agaaggctca ctggaatttc ccgcattgtt tgcgtcaata
     4801 aaaaaatgcc catcgcatca gcaaattgtg gtattatcac accccaagta gtatcaatta
     4861 aattaagttt tgcaatcaac aaataattag gaatcatcgc aacagtaaaa ggaatgaaca
     4921 tcgtaccaat gatgataaag taccagattt tcttcccttt gattcggcaa taagtaaaac
     4981 tatatgccgc aaaaaaagct acgcctaact taacaaccgt tataattgta gcaataaaaa
     5041 aagtatttct cgtaattttg ataattggaa gattttcgaa caaatagcaa aaattttcca
     5101 aggtaaacgg ctgtggtatc aaactagtaa tcgtctgata ggcatctttc aatggcttaa
     5161 aagaattggc cataataaac aataatggat agataataat gattatgatg aacataaata
     5221 agagatgtgg caaccagcgt ttaattttca tagtaaaccc ccttttccac aaatttaaat
     5281 attaccccta agaaaacagc aaatattaat aaagttaaaa ctgaaattgc tgaagattct
     5341 ccgactttat acatttcaaa agccttttga taagcataat agatcacgtt tgcactacca
     5401 ttattcggtc caccacgagt gatgatctta atcggagtaa acacatactg cagaccttga
     5461 acaatggtca tcaccaataa atatagtcct gtcgaactac tcataggcaa cacaattgta
     5521 aaaaaaatgc gccagatggg tacacgatcc aacttagctg cttcgatcat ctcttgatca
     5581 attcccacaa ttgctgcaaa aatgacaatg aaattataac caaaaacttt ccaagcagta
     5641 atacctgcaa tcacagcaat tacccaacct tgtaaattca tccacatagg cagtcttaaa
     5701 ccaaaatttc ccaacaagac agcaattggt cccgataccg gatttaatat ccatgtaaaa
     5761 accatccccg ccactaccaa agaaatcaca gctggaataa aaaataatgg tttatacaaa
     5821 ttctgccctt tttctataac tattcctaat aaaaaggatg taatatatgg gaacacacag
     5881 ctcagcaaga caaatagaac ggtataggac aaagtattct tcaacacttt atgaaagact
     5941 ggatcagtca gcacctttac atagttggca aagccaacaa attctttagt gggggccacc
     6001 atattccagt caaaaaaact caaatagatc gtataaatca atggataaaa aacaaacacg
     6061 cccaacaaaa taattgctgg cgccagatac aaataaccta catagcgctt catttccgac
     6121 agcctcctca tccttatgtc aaaagcagac gaccttgttt ttcctttctt acaggcgctg
     6181 tcgtctgctt gctttgctta tttgagtaac tcattaattt ccttttgagt agctttcatc
     6241 gtttcattga cggaattacc tgataaaatt ttatcccgca tatccaacaa tttttgttca
     6301 gcttctaatc cagcatctcc aggaaatgaa ctccaactga ccatcttatc catctgatca
     6361 attgctggta tcatcatctg attttcagct aagaaagctt ttaatccatt ttgggcgtct
     6421 gcaacatctt tacgtggtgg taaataacca gtaccttctg tccatttagc taaagattca
     6481 acactataaa gatattttaa gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat
     6541 gtaacagcta acattgcgcc accagcaggt agtttaactt ttttcccttc ccaacttggt
     6601 gactcaactg ctttagcagt aaaagtacta tttttttgaa tgttgtccct ttgagcaata
     6661 gtggtataga ccatcgcgac gttaccatca ataaagcttt gcacaccttg ttcccacgta
     6721 gtgtgtaatg ccgtctggtc ttttaccatc ctttgatata attcataagc agcgatacca
     6781 tctttagaag caaaggtagc tttgccatct tttattacac ttgccccatt actttctaaa
     6841 attgcttgtt gtgcccaatt atcagcaggt tcttgaatat acacgccata ctttccagtt
     6901 ttttctttta ttatcttaga aaaagtttga acttcttccc aagtttttgg accattttca
     6961 tccaagcccg ctgcttttaa tatatcttga tttaaatata aaaccggatt actaatggag
     7021 taaggaatac ccacttgttt accatctttt tcagacagtg ccatgatatt gtctaaaaaa
     7081 tgatctttaa aataatcagc attatccgga tccacttctt ctactaaaga ttgaggatta
     7141 gtatatttga aattttctgc aaaataatct ttaaaagccc atcccacttg tactacgtcc
     7201 ggtatttctt tggaggcaac agaagcttgt aaattttgca tcaagccttt atacatatca
     7261 ggattatatt ttgctacaac cttcactttg tcttgagatt gattgtattt atctactaat
     7321 tcattcactg tttttccacc tagggtatcc gcattaacat gccaatactc tatcgttaca
     7381 cgaccctccg atttttttgt tgtcttggta ttcccactac cacaagctgc caaagagatg
     7441 acacttaaaa cagacacaac ccctataaca agatttttct ttaacataac attaacttcc
     7501 tcctaatttc tttatggcca tttttctaat caagaaatat tatgaactac caatgtaaat
     7561 ttctagttaa ttttctgtaa ataattcgaa atttgtgcat ttcggggtac agggtcactg
     7621 gacatatcca tcaagctaag aataaagccc ccatatggtc atgtatccca tctccaaatc
     7681 tcttaactcc tctgttttga ttataaaata gtttattctg tgctcctttc ttccataaat
     7741 aggcccatgc acaacgtgag gttatattat aaacaccaca gcgcgcaagg tactcaatcg
     7801 tttctagttc attgactgtc catacagaaa atgtcaatcc tagctgctgc cccacttcac
     7861 gggccaactt agttaaccat ccttcatgaa tattgacgat ttgaacgctt tgataggcta
     7921 atttctgtaa ggtatcgatc aaatctgctt cggtaatatc cccaagtttt ttcccttcga
     7981 actgattttc aatattataa aatatatgct ttccccaaat cgcagcccaa gataattgta
     8041 catagccact aagaacaatt tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg
     8101 ccatttccaa tccctcttct tttaaatcac agttaatggt catggttgcc ttctcattgg
     8161 ccactgcttg aaacacctgc tcaagggtta ccgaatctgg gacaacttct tacctgtcat
     8221 gggctagtat tagcgtacca tctgtttgct gacgcacatc tacttcaaaa caaagctggc
     8281 gatccaaaca aaagcgaaga aatgcccagc tattctcctc cgtattgtca ctaccagagt
     8341 gtgcagtgat catggtcatt ttgacccctc ctttaattcc gtgattttct cccacacgtc
     8401 cactataccg atgaagcgtt aatttcttgt taatttcttg taaattccct gcgttttttc
     8461 agtaaactag ttgtaaatct tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg
     8521 cccttctagc tagtttcacc aattaagtag ttctacattc cccgctttta acctactaca
     8581 cctttttttg ttttcagcta ttccttcttc ttgctgaaag tccttgaggt tctttcttac
     8641 aggataccat caaggtgttc aatggcttat tcccattgaa tgaccgagac ttactatcag
     8701 atttctgctc gttgaaccaa gtctttgata caggatttct tccgtcatct tctcacaaat
     8761 accaccttgc gtttctctag cgattggtcg atgtgcaccc tcccagtgga ctgtcactac
     8821 ccctttttgc tatgcctagc ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DARDHD010000109         8855 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000109 DARDHD010000000
VERSION     DARDHD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.109"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..661
                     /locus_tag="SDA06_001969"
     CDS             <1..661
                     /locus_tag="SDA06_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG4229233.1"
                     /translation="KISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFES
                     HKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFM
                     SNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELA
                     DIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLD
                     K"
     gene            728..1222
                     /locus_tag="SDA06_001970"
     CDS             728..1222
                     /locus_tag="SDA06_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4229234.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1278..1916
                     /locus_tag="SDA06_001971"
     CDS             1278..1916
                     /locus_tag="SDA06_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229235.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1988..2383
                     /locus_tag="SDA06_001972"
     CDS             1988..2383
                     /locus_tag="SDA06_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229236.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2367..4820
                     /locus_tag="SDA06_001973"
     CDS             2367..4820
                     /locus_tag="SDA06_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4229237.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4817..6844
                     /locus_tag="SDA06_001974"
     CDS             4817..6844
                     /locus_tag="SDA06_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229238.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6841..7863
                     /locus_tag="SDA06_001975"
     CDS             6841..7863
                     /locus_tag="SDA06_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4229239.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7880..8809
                     /locus_tag="SDA06_001976"
     CDS             7880..8809
                     /locus_tag="SDA06_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229240.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         3011 a         1490 c         1767 g         2587 t
ORIGIN      
        1 gaaaatatca gaggtaactt tagatgagac tgacgatttt acttattata aaggatataa
       61 aaagggctgg aatacgcttt gcacatatct taaaataaga gttccttttg atgatttaga
      121 cttttttgaa agtcataaag atttgattac ccagacagca agctccatat atgataaaca
      181 aggtgataat gtattagtgg atactatttt agtaccactt ccagagaatt acgaagtaat
      241 taactttagt cagttgaaaa ttagtgatgt ggtttcacaa gctattgaag acgctgagtc
      301 ttttatgagt aatggtgaat atcaacgtgc ctttgacaga gttcacactg cttttcatgg
      361 atatttaatt gaaatactaa aaaaatatga aattacagtt cctagagacg aaaatctatc
      421 taaattatat agtaggatac aacaattaat tgaaaaagaa attcaaccta ctgaacttgc
      481 tgatatagtg aagacaacga taaggtcatc aaatggaatg attagctctc ttaatgaagc
      541 aagaaatagg cattcacttg cacaccctaa tacaaatatt attggaaaga gagaagcaaa
      601 actaataata ggtatttcaa gtacagttac agattatatt agcggatacc ttgataagta
      661 gcgtcattaa ctgattgcta ctttttttat gactattttt ataaaaagga tgtgataaga
      721 attggaaatg aaagtctatg tggcaaattt aggacggtat aatgaaggtg aattagtcgg
      781 agcatggttt actccaccaa ttgatgagga agaaatggca gaaagaatcg gtttaaatga
      841 ggactacgaa gaatatgcaa tacatgattt tgagcttcct tttgatgtgg acgaatacac
      901 gccgattagt gaaatcaacc gtctatgtga agccattcaa gaaatcgaag gtacacctat
      961 ttataatgaa ctcaaagaaa ttcaaggcat gtggtttagt agcttggaag aattactgga
     1021 aaacaaagag gatattcatt gttatagtga ttgtgattcg atggaagatg tcgcccgtta
     1081 ttacgtggaa gaaacaggac agcttggtga agtcccttcc aatttacaaa attatattga
     1141 ctaccaatct ttgggacgtg atatggaaat agagggtaat tacctagtta cctctcatgg
     1201 ggtatttgaa tattgtcagt aagtttaaca atccgtgtta taattaaatt aataaatagc
     1261 acggagaggt ataaattatg gtaaatgtat ttgaaaatct taaactatta aaactagaca
     1321 tgaaagataa aggctgggtg atagattcct tttactttcg ttacaaacag caaaattata
     1381 ttgtacttgt taagctattt gaaaaagagg aaaaagttcc agaatatgct ctgttaaaac
     1441 tagaattttt aaaggaaaac gatttttcag atatgctagc tgtctatgct aattcggtaa
     1501 aattatatac agacacgaaa acaataagag aatattttgg tatcgaatac agtagtaact
     1561 tgggagatgt tttatttcaa tttagccaaa ctcttgctcg atttattccg acagaagtta
     1621 gtgagaagaa aaatgaagac caaaaagaag ccatgtgttt ctcattaagt caaagtgatt
     1681 cagaagatcc cagaaaaaaa tattgttttt cagttagaag aaatccttta aagggaaatg
     1741 gtgagctggg aaaacgaagt ccttttaatg acaataaaac gagattgtat agacccagct
     1801 tatatgaacg attagggtca gataccaatt taagtttcag atattcaatg aatccagatg
     1861 atgaagaaac tgatgaaggt attattgcaa aatggacaaa aaataaaatt gaatagttaa
     1921 agcattgaaa gagcaatcac ctattttgat tgttcttttt ctatttttac ggaaaggacg
     1981 tgaagacatg aaaaaaataa gaagttacac cagtatttgg tcagtagaaa aagtgattta
     2041 tgcgattaat gatttacaac tccctttccc aattaccttt acgcaaatga catggtttat
     2101 cgtttctttg tttgtcgtca tactctttgg cagtttacca ccactttctc tgattgacgg
     2161 agcactactc aaatacttag ggattcccat tgttctcact gggttcatgt gccaaaagac
     2221 ctttgacgga aagaaaccct atggatttct aaagtctatc attacctatg ctctacgccc
     2281 taaagtaacc tacgctggca aacaaatacg attaaaaaaa gaaacaatga atgaatcgat
     2341 tacgattgta aggagtgaac aactagatgg catatccgat taaatatatg gaagataatc
     2401 tcgtatttaa tcatgatggc gaagtattcg cttattatga attaattccc tataattaca
     2461 gttttttaag ttccgatgaa aaaattcaag ttcatgataa tttctgtcaa ttgattgccc
     2521 aaaatcgtga tggcaagatt catgctctgc aactagcaac cgaatcaagt attcgagcca
     2581 cacaggaaca atcgaaaaaa gaaatcactg gacgattaaa agagatagct tacgaaaaaa
     2641 tagatgaaca gaccgaagcg ttaatttcta tgattggtga caatcaagta gactatcgct
     2701 tttttattgg ttttaaactc tacctcaatg aacaggaagt atccgtaaaa aatgttggga
     2761 aagaaattca aaaagcattt gcggaattct ttcatggtgt gaatcacaaa ttaatgggcg
     2821 attttgtttc tatgcctatg gcggaaatcg aacgcttttc taagatggaa cagttgttag
     2881 aaagtaaatt atccagacgc tttcagattc gtaaattgga gaaagatgat tttggttata
     2941 ttattgaaca tctttacggg caaacaggtg tcccttttga tgactacgac taccatttac
     3001 cgaaacgata cgaagataaa aaagtgttgg taaagaaata cgatattctc aaacccactc
     3061 gctgtttaat cgaagaaaat caacgctatt tacggattga acaggaagat accactactt
     3121 atgtggcgta ttttacaatt aatagtattg ttggtgaatt ggatttccct aatagtgaaa
     3181 ttttttatta ccagcaacag cagtttactt tccccgttga taccagtatg aatgttgaaa
     3241 ttgtcgccaa taaaaaagcc ttatctaccg ttcgcaataa aaagaaagaa ctgaaagatt
     3301 tagatagcca cgcatgggaa agcgacaacg aaaccagtac taatgtggtg gacgctttag
     3361 aatctgtcga tgaattagaa agtaatttag accaaaccaa agaatctatg tacaaactaa
     3421 gctatgtggt acgggtatca gcaaagaatt tggacgagct gaaacaacgt tgtgatgaag
     3481 taaaagattt ttatgatgat ctgtctatca aattagtacg accttttggc gatatgctag
     3541 gcttgcataa tgaatttatt ccctcaagca agcgatatat caatgactat attcaatatg
     3601 tcacgtcgga ttttttggct ggtcttggtt tcggtgctac acaaatgatt ggtgaaaatt
     3661 ctggtattta cgtcggttac aatctcgata caggcagaaa tatttatctc caacctagcc
     3721 ttgcaagtca aggaattaaa ggctctgtaa caaatgcatt agcgtccgct tttattggtt
     3781 cattaggtgg cggaaaatca ttctctaaca atatgctggt ctattattca gtactctttg
     3841 gcggacaggc aaccattgtt gacccaaaag ctgaacgtgg tgaatggaaa gaaaccttac
     3901 ctgatattgc ccatgaaatc aatattgtta atctcacgag tgagaacgac aataaagggt
     3961 tacttgaccc ttatgtgatt ttaaaactcc cgaaagattc agaaagcctt gccattgata
     4021 ttctcacgtt cttaacaggt atttccagcc gagatagcga gaagtttcct gtcctgcgta
     4081 aagctattcg gagtgtaact caaagcaaac aacgagggtt gttattggtg attgatgaat
     4141 tgcgaaaaga agatacgccc atcagcaata gtattgctga ccatatcgaa agctttgtcg
     4201 attatgattt tgcccatctg ttattcagtg atggcagtgt cacccaatcc attagcttgg
     4261 aaaaacaact caatattatt caagtggcag atttggtctt acccgattcc aatagctctt
     4321 ttgaagaata cactaccatg gaattattaa gtgtggcaat gctgattgtc attagtacct
     4381 ttgctttaga ctttatccac tctgaccgtt ccatttttaa aattgttgac cttgatgagg
     4441 cttggagttt tttacaagtg gcacaaggaa aagctctatc tatgcgattg gttcgagcag
     4501 gacgtgccat gaatgcaggg gtctatttcg ttactcaaaa cgcggacgat ttactagatg
     4561 aaaagatgaa gaataatatt ggcttgaaat ttgcctttcg ttccactgac ctagtggaaa
     4621 tcaaaaagac cttagaattt tttggtgtgg acaaggaaga tgaaaacaat cagaaacgct
     4681 tacgtgaatt agaaaatgga caatgtttag tttcagacat ctatggtcgt gtaggggtta
     4741 tgcagtttca cccgattttt gaagagttgc ttcatgcctt tgataccaga ccgcctgtgc
     4801 gaaatgaggt ggaagaatga cacaaaaaca aaagaaattt cttcactata gttggataac
     4861 gctcctttgt gtgggcgtta ttttactttt attaggcacg ctgggcaatg cagtacaagc
     4921 gacaggctta gttgatgaaa ccattgatac aagcaatgaa tactccaagt atggtttgaa
     4981 tcaatatcag cttgattatt atgtggacaa tagctggggc tggcttccat ggaattggag
     5041 tgatggcata ggacaatcgg tgatgtatgg attgtatgcc attactaatt ttatttggac
     5101 aatttctctt tatctatcga acgccacagg ttatttggtg aaagaagcct atgccttaga
     5161 tttcattagt caaacatccc aagcaattgg cgaaaacatg cagacgattg caggcattac
     5221 accgaatggc ttttccagtt cagggtttta tgtcggtttc ctccttcttt ttattctcat
     5281 tgttggaatc tatgttgcct atacaggatt ggttaagcga gaaacgacga aagtgatacg
     5341 agctgtttta aactttgtcg tggtgtttat tctatcggct tcctttattg cgtatgcacc
     5401 cgattatatt gcgaaaatca atgatttttc caaagatgtc agtaccgcca gtctgaatat
     5461 tgggacaaaa attgttcttc ctaacacgga tagtcaaggg aaagacagtg tggacatgat
     5521 acgtgacagc ttattctctg ttcaagtaaa gcaaccatgg ctattgcttc aatatggctc
     5581 cactgatgta gaagcgttag gggaagaacg tgtggaaagt ttattgtcta ccagtccaga
     5641 tacaaacaat ggtgaagata gagaaaatgt agtgattgaa gaaattgaag atcatgacaa
     5701 tacctattta accctaccga agacgatttc cagattagga acagtcttct ttttgtttgt
     5761 attcaatatt ggtatctcga tttttgtttt ccttctaaca ggaatcatga ttttttcgca
     5821 agtgctattt attattttcg ctatgttttt gccaatcagt ttcctattaa gtatgttgcc
     5881 gagttttgat ggcatgggca ggcatgccat taccaagtta tttaatgtga ttatgacaag
     5941 ggctggtatt accttgatta ttaccgttgc ctttagtatt tccactatgc tttattcgct
     6001 gtcagcaagt tctccctttt tcatgattat gtttttacaa attgtgacct ttgcagggat
     6061 ttattttaaa ctgggcgatt taatgagcct attcacttta caaagcaacg actctcaaaa
     6121 catgggcaga caagtgtttc gcagacctcg tcaaatgatg aatcgacaaa ccagaagatt
     6181 tcaacgcagt atgagtaaga tttttcgtgg aaaagcaggg tcaacgaagc aaaagacaat
     6241 gacaacaaag cagaatcaaa ccaataaagc taaccatact cgtcaaaatg aacggactac
     6301 accaaagaaa gaaacagcac tttccaatgt gaaaaagcaa caaggcggta tcgggcaacg
     6361 attaggtgaa aagacagccc atgtattgga cacgaaaaat cgtctagtca ataaaagaaa
     6421 gcaggcaaaa caacagatac aggcgacacc aacccatgtc aaatatgccc ttcatcaagg
     6481 aaaagagaat gtcactcata atgtaacaga ctttaaacaa tctatggcag ataccaaagc
     6541 tcaacgcaaa caacagcgta tgaaccagca ggagcaaaaa cgaaagaaca ttgctcaaaa
     6601 gcgtttggag ctggataaag caaaagaaaa aagacgaaaa gaaaacccat catctcctgt
     6661 tcagaataac cagcagcatg ttcgcaagcg tcagcaaacc gtcacgcctg ttttgtcaca
     6721 aaacactaaa cgtgaaaatc atcagcgacc attgaaaaaa gagcaaaaac aaaagacacc
     6781 accgaagcaa ttcaatcaga ggaagctaaa taaaagaacg gataagcgag gacaacaaac
     6841 atgaagctaa aaataacagt gatttgtagt gtggtcttct ctctcttctt ttttctctta
     6901 ttattcctag tcattttctt tgcagatgat acggacagtg gcgaaaacaa caaggattct
     6961 tctattccac aaggcggtgt gaccgtttca ccagaagtgt tggcacaccg tccgttgatt
     7021 gaaaaatatg gtaaggaata tgggattgaa gactatgttt cttatatctt agccattatg
     7081 caagtggaat cgggcggtac agcagaagat gttatgcaaa gttccgaatc tttgggttta
     7141 ccgcccaaca gtttgagtac cgaagagtcc atcaaacaag gggttaaata tttcagtgaa
     7201 ttattaacca gtgcagaaca acaaggagtg gatatagatt ctgttatcca atcttataat
     7261 tatggcggtg gctttttaaa ttatgtgagg agtcatggga aaaaatatac ctatgaatta
     7321 gccgaacagt tttctaaaga gaagtcgggt ggtcaaaaag cagactatcc caatcctata
     7381 gccatacctg tgaatggtgg ttggcgatat aactatggaa atcagtttta tgttcagttg
     7441 gtttcacaat acctaaccga cacttcccca acagaatttg atgatgaaac cgtgcaagtc
     7501 attatggacg aagccttgaa atacgaaggg tttccctatg tctttggagg ggcgtcgcct
     7561 accacctctt ttgattgtag tggcttaata caatgggtgt atgacaaagc tggcatttct
     7621 ctccctcgtg tcgcccaaga tcagtatgac gctacgcaag aaatctctat ggaagaagcc
     7681 caagcaggcg accttatctt ctttcattca acctataatg caggcacata tgtgactcat
     7741 gtggcgatat atttagaggg aaatcgcttt tatcatgcag gcgacccaat cggttatggc
     7801 gacttaagca gtcgttattg gcaagaccat ctgattgggg ctagacgtgt tattcataac
     7861 taagaaagga atgaatcaaa tgattataaa aatcgagcga aaagaaaaga aggagaaacc
     7921 aaagaaatct aaaaaaccga gcaagcaaag aaaagttccg atgattaaag ttggcacgca
     7981 taagaaatta acctttgttt tatgggtttt attgattggt agtgtcggat ttggaatcta
     8041 taaaaatttt actgccattg acactcatac tgtccgtgaa acagaaatca ttaaacagca
     8101 gattgtcgat accaaccaag tagaaagttt tgtgaaatcc tttgcgaaag actacttttc
     8161 gtggcaacaa tcccaagaag ccattgataa acgaaatgaa aaattgaccc attatttgac
     8221 ggaagaattg caggtattaa atgaagaaat gattcgtaag gatattccta ctagttctag
     8281 tgtgaacgat atacaggtgt ggcaggtttc tcaagtgaat gagaacacct ttgaggtact
     8341 gttttctgtt gaacaagtta ttaccgaaga caaagacaag gaaactatat cgtctagctt
     8401 tcatgtggtt gtccatatag atgaatcaga taatatggtt attatcaaaa atccgacaat
     8461 gagtaagaaa cctcaaaaat cggactacca acccaaacag ctagaaagtg accatacagt
     8521 agatacagaa acgatggacg aaatcactag ctttttagaa acgttcttcc agctctatcc
     8581 taccgccaca gaaaaagaat tgacttacta tgtaagtaat catgtgttac ctatgattaa
     8641 caaggaatat gttttcgagg aattggtgaa tcctatcttt accagaaaag ataaccaagt
     8701 aatcgtgaat gtcgctgtga aatatttaga tcaagaaaca aaggcaaccc aaatctcgca
     8761 atttgagttg atattagaaa aacaagataa ttggaagatt gtaaaataac aaatattggt
     8821 acatgattac agatactttg taatcatgta ctctt
//
LOCUS       DARDHD010000110         8826 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000110 DARDHD010000000
VERSION     DARDHD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.110"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..307
                     /locus_tag="SDA06_001977"
     CDS             8..307
                     /locus_tag="SDA06_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229241.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(404..838)
                     /locus_tag="SDA06_001978"
     CDS             complement(404..838)
                     /locus_tag="SDA06_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229242.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1070..1780)
                     /locus_tag="SDA06_001979"
     CDS             complement(1070..1780)
                     /locus_tag="SDA06_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4229243.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1770..2630)
                     /locus_tag="SDA06_001980"
     CDS             complement(1770..2630)
                     /locus_tag="SDA06_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG4229244.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2635..3273)
                     /locus_tag="SDA06_001981"
     CDS             complement(2635..3273)
                     /locus_tag="SDA06_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG4229245.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3288..3587)
                     /locus_tag="SDA06_001982"
     CDS             complement(3288..3587)
                     /locus_tag="SDA06_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229246.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3630..5114)
                     /locus_tag="SDA06_001983"
     CDS             complement(3630..5114)
                     /locus_tag="SDA06_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4229247.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5134..5595)
                     /locus_tag="SDA06_001984"
     CDS             complement(5134..5595)
                     /locus_tag="SDA06_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4229248.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="SDA06_001985"
     CDS             complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="SDA06_001985"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG4229249.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6898..7668
                     /locus_tag="SDA06_001986"
     CDS             6898..7668
                     /locus_tag="SDA06_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4229250.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7941..8768)
                     /locus_tag="SDA06_001987"
     CDS             complement(7941..8768)
                     /locus_tag="SDA06_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229251.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2676 a         1859 c         1384 g         2907 t
ORIGIN      
        1 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
       61 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      121 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      181 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      241 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      301 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      361 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      421 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      481 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
      541 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
      601 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
      661 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
      721 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
      781 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
      841 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
      901 atcacatata actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta
      961 gaatgcatac tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     1021 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     1081 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     1141 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     1201 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     1261 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     1321 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     1381 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     1441 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     1501 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     1561 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     1621 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     1681 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     1741 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     1801 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     1861 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     1921 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     1981 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     2041 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     2101 tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     2161 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     2221 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     2281 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     2341 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     2401 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     2461 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     2521 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     2581 catgaaatat ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc
     2641 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     2701 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     2761 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     2821 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     2881 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     2941 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     3001 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     3061 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     3121 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     3181 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     3241 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     3301 tggtaccact ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg
     3361 ataccgataa ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact
     3421 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     3481 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     3541 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     3601 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     3661 tagtcttcta caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata
     3721 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     3781 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac
     3841 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     3901 gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca
     3961 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     4021 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     4081 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     4141 atcgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     4201 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     4261 aaactctgcc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     4321 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     4381 atcgacaaga atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta
     4441 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     4501 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     4561 actgttaagt tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat
     4621 gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     4681 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     4741 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     4801 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     4861 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta
     4921 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     4981 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     5041 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     5101 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     5161 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     5221 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa
     5281 aaacaaacta gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt
     5341 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     5401 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     5461 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     5521 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     5581 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     5641 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     5701 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     5761 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta
     5821 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     5881 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     5941 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     6001 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     6061 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     6121 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     6181 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     6241 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     6301 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     6361 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     6421 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     6481 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     6541 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     6601 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     6661 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     6721 aatattaatg tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat
     6781 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     6841 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     6901 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     6961 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     7021 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     7081 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     7141 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     7201 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     7261 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     7321 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     7381 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     7441 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     7501 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     7561 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     7621 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     7681 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     7741 cgctacgctc aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata
     7801 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     7861 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     7921 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     7981 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     8041 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     8101 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     8161 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     8221 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     8281 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
     8341 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
     8401 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
     8461 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
     8521 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
     8581 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
     8641 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
     8701 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
     8761 ttaatcaaaa caatttcctt ccctttaaaa aattatttct cctttatatc atacacaaaa
     8821 aaaagt
//
LOCUS       DARDHD010000111         8798 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000111 DARDHD010000000
VERSION     DARDHD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.111"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="SDA06_001988"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="SDA06_001988"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG4229252.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SDA06_001989"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SDA06_001989"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG4229253.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="SDA06_001990"
     CDS             complement(3070..4323)
                     /locus_tag="SDA06_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4229254.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="SDA06_001991"
     CDS             complement(4329..5252)
                     /locus_tag="SDA06_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4229255.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="SDA06_001992"
     CDS             complement(5473..7965)
                     /locus_tag="SDA06_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4229256.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="SDA06_001993"
     CDS             complement(7972..8439)
                     /locus_tag="SDA06_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG4229257.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8575..8648)
                     /locus_tag="SDA06_001994"
     tRNA            complement(8575..8648)
                     /locus_tag="SDA06_001994"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8612..8614),aa:Arg,seq:acg)
     gene            complement(8706..8779)
                     /locus_tag="SDA06_001995"
     tRNA            complement(8706..8779)
                     /locus_tag="SDA06_001995"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8743..8745),aa:Pro,seq:tgg)
BASE COUNT         2440 a         1867 c         1461 g         3030 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc
     8581 caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct
     8641 atgggcgcat attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa
     8701 aaaaatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac
     8761 caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DARDHD010000112         8737 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000112 DARDHD010000000
VERSION     DARDHD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.112"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            343..612
                     /gene="rpsN"
                     /locus_tag="SDA06_001996"
     CDS             343..612
                     /gene="rpsN"
                     /locus_tag="SDA06_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4229258.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            627..806
                     /gene="rpmF"
                     /locus_tag="SDA06_001997"
     CDS             627..806
                     /gene="rpmF"
                     /locus_tag="SDA06_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4229259.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            838..1215
                     /locus_tag="SDA06_001998"
     CDS             838..1215
                     /locus_tag="SDA06_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229260.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1236..1385
                     /gene="rpmG"
                     /locus_tag="SDA06_001999"
     CDS             1236..1385
                     /gene="rpmG"
                     /locus_tag="SDA06_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4229261.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1409..2392
                     /locus_tag="SDA06_002000"
     CDS             1409..2392
                     /locus_tag="SDA06_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4229262.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2422..2544
                     /locus_tag="SDA06_002001"
     CDS             2422..2544
                     /locus_tag="SDA06_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4229263.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2562..4085
                     /locus_tag="SDA06_002002"
     CDS             2562..4085
                     /locus_tag="SDA06_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4229264.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4109..4348
                     /locus_tag="SDA06_002003"
     CDS             4109..4348
                     /locus_tag="SDA06_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229265.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4541..5002)
                     /locus_tag="SDA06_002004"
     CDS             complement(4541..5002)
                     /locus_tag="SDA06_002004"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4229266.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5197..5415)
                     /locus_tag="SDA06_002005"
     CDS             complement(5197..5415)
                     /locus_tag="SDA06_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229267.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5533..7149
                     /locus_tag="SDA06_002006"
     CDS             5533..7149
                     /locus_tag="SDA06_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4229268.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7390..8730)
                     /locus_tag="SDA06_002007"
                     /pseudo
     CDS             complement(7390..8730)
                     /locus_tag="SDA06_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         2967 a         1359 c         1756 g         2655 t
ORIGIN      
        1 cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt catattgccg
       61 ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac taataaaagt
      121 gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga ttatctctat
      181 gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt tcatgttatt
      241 tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt aacgattacg
      301 atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa aaagtcgaaa
      361 attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa acgtcaggaa
      421 ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc aaatccgaat
      481 cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg aaagttcggt
      541 atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg agtaaaaaaa
      601 gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca tctaaggcga
      661 agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca tttgacgaag
      721 cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat aatggaaaga
      781 aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg aatcaaaatg
      841 gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga aacgacccag
      901 caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat tattactgaa
      961 acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta ctatgaggac
     1021 caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga gtatgacgag
     1081 gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa agaaaattta
     1141 gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat tcaagcatat
     1201 cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt attttggaat
     1261 gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat aaccctgaac
     1321 ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt aaagaagtga
     1381 aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg tttctgggtt
     1441 tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac cctaccccag
     1501 ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc acaaattatt
     1561 agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt gcagtcttcg
     1621 cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac atcaacaacc
     1681 aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa tcattcaaac
     1741 acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat tgactgttat
     1801 tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc aacttgtcat
     1861 ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac taaaaaaatt
     1921 acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca cacatagtga
     1981 aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct tagtttttga
     2041 aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc atcaagtaca
     2101 tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt ttttactcca
     2161 atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta aaggattgat
     2221 tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac aggttaattt
     2281 tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa aaatgttgga
     2341 tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat aatgataagt
     2401 agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata tcgacgactt
     2461 aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga acataaacga
     2521 aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa atcagtgtag
     2581 gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact aatgatgcaa
     2641 aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat ccaatgtatg
     2701 atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt ccagcgggat
     2761 cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat gatgctgatg
     2821 tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa gaaagttgga
     2881 agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg ccaggtagtg
     2941 aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat cctcatacgt
     3001 gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag ttagttaaat
     3061 tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg actaaactga
     3121 aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa agttttgtga
     3181 cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa gttcctattg
     3241 ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta aaagagtatg
     3301 ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat aaaattgcaa
     3361 aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt gaaagtctta
     3421 caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac aatctaaaag
     3481 cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa gaagcgaagg
     3541 atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat cgcgaattga
     3601 gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga attttggatg
     3661 aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag tacaaagatt
     3721 attatactac tggctataag acagatattg atactatcaa cattaaagac aacaccattg
     3781 attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga tacaaaattt
     3841 tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat gatgcaggtg
     3901 ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt aaggcagagc
     3961 atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa ttgcataatt
     4021 ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa atgatggctc
     4081 attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt ctatggattc
     4141 caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat taaatgggag
     4201 tacattagct gatattgctg caactttaca tgttagtatt gacacagttc atagagtatt
     4261 aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta gacgtttagc
     4321 aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca tttttccctt
     4381 atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg caacacttta
     4441 tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct gttattcagt
     4501 ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt cgattttggc
     4561 aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca agggcatgag
     4621 gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa tgattggaga
     4681 attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata ctgcttaaaa
     4741 gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc tttcctgcta
     4801 aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca gttttgccag
     4861 ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat tttggacggc
     4921 catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt tttgtatgtc
     4981 ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt gagaggggtt
     5041 ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta attgaaaagt
     5101 ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga aaatcgacaa
     5161 ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa cgtccgcatt
     5221 tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt ttttcttgtt
     5281 tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc caattatact
     5341 aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta tagtaagatg
     5401 acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact tatactagtt
     5461 ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc aaggtataga
     5521 aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct tggttcagga
     5581 aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat tgctgtcatt
     5641 gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa aggttttaaa
     5701 agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac attacgcgaa
     5761 gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca aattgttatt
     5821 gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt agctaaaaac
     5881 cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat agtagtggat
     5941 gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag aaaagctgaa
     6001 ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca attcgagtgt
     6061 tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat ttacaaaatt
     6121 aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat taaaggtgaa
     6181 gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac ttcctattca
     6241 gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga aacagaagaa
     6301 tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca aaaatttgcc
     6361 catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt tctttggttt
     6421 gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa aagtattgaa
     6481 tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat tatacaaaaa
     6541 aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa taaactagtt
     6601 tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg ttgtttaact
     6661 gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa aaagtctaat
     6721 atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa gataggcgat
     6781 gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt attctctata
     6841 gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg aattgatggc
     6901 aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa ttatcatcct
     6961 gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt taaagaagat
     7021 aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg tcaaacgagt
     7081 atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac agattactac
     7141 atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat tattattaat
     7201 aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag aagcggtgac
     7261 aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc ggttagatta
     7321 cttattttgg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat aatcgagata
     7381 ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt tagtcagttg
     7441 ggtagtattc ttagttttct taaaatattt tcctcgatat aaaaagattc gttctcggta
     7501 gttttgaaag tttctatagc cataggcaat tcgttttagt actttgatat ggttattcaa
     7561 acattctata gggccgttgg aatagggaac gcgcaacgct cgtttgattt ctgtttggta
     7621 tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt agtcttcttt
     7681 taggagcgcg acgaattcag gaacatcttg tcttctgata acggaaagaa aatcctggta
     7741 gacttcgtag cctcgcttta aggaatcatc ataagataat agccgatcga ctatctctgc
     7801 ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgacctt tcaattccca
     7861 cgcattcttt tgaagtagtt tccagtattt ctttaagtgc ttcgaccgac gatccccttt
     7921 tttaaaggtc ttcattactt gaattcggtg gttggtaaaa gcccgaccaa tgtgttggct
     7981 aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca ccaatgtgcg
     8041 atagggtgta tagaaatctg aaaccactag acgcacttgt tcacggactt ttatagggta
     8101 gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat caatcagttg
     8161 atgggtcttg ccgtccatca tgacaaagct catggctccc gagacatttt tcactgattt
     8221 gaattcatcg aaacacacca cttctggtaa cagctggctc aaagaaatgg ttttaggccg
     8281 aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac gtgcgatcaa
     8341 agacatagaa agaggttcgc ttaatagctc aagaatggct tgtttgactc tgcgagcaat
     8401 ggagcagcga cgatcactaa ctgaatcttc tgctaaaaaa gtctttccac agtgtttaca
     8461 tttgaagcgc tgtttgttaa tccgcagaat ggtcttgtag ttactgacat cattcaataa
     8521 gatggatact tttttccagc cctatttaat gatcgtttgt tcattttgat agtggcgatg
     8581 aaagcattcc tccggttgat aggtcaatgt gccatcaaaa acaaaacatg tttcgtcatt
     8641 gattcgctct ttgcgaaagg catcttcatg aaagataaga tttaggtcta gaatatctag
     8701 taactctttg gtaaactctg aataggacat cttgaaa
//
LOCUS       DARDHD010000113         8644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000113 DARDHD010000000
VERSION     DARDHD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.113"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(123..206)
                     /locus_tag="SDA06_002008"
     tRNA            complement(123..206)
                     /locus_tag="SDA06_002008"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Leu,seq:cag)
     gene            734..1234
                     /locus_tag="SDA06_002009"
     CDS             734..1234
                     /locus_tag="SDA06_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229269.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1424..1768)
                     /locus_tag="SDA06_002010"
     CDS             complement(1424..1768)
                     /locus_tag="SDA06_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG4229270.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1922..2842)
                     /locus_tag="SDA06_002011"
     CDS             complement(1922..2842)
                     /locus_tag="SDA06_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4229271.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3029..3520
                     /locus_tag="SDA06_002012"
     CDS             3029..3520
                     /locus_tag="SDA06_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG4229272.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3544..5919)
                     /locus_tag="SDA06_002013"
     CDS             complement(3544..5919)
                     /locus_tag="SDA06_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG4229273.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6245..6871
                     /locus_tag="SDA06_002014"
     CDS             6245..6871
                     /locus_tag="SDA06_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4229274.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6894..7598
                     /locus_tag="SDA06_002015"
     CDS             6894..7598
                     /locus_tag="SDA06_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4229275.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7790..8623
                     /locus_tag="SDA06_002016"
     CDS             7790..8623
                     /locus_tag="SDA06_002016"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG4229276.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2622 a         1647 c         1536 g         2839 t
ORIGIN      
        1 tttttttgat ttatttgaat ctggttccgt tgtttaaaat ttttcctata ttcttttaga
       61 taatatgtta ttttctttct catcgctcca ggtgtacctt ctctttataa aaaaacaaat
      121 tatgcggcta agaagactcg aacttccacg aggtcaccct cactagcgcc tgaagctagc
      181 gcgtctgcca attccgccat agccgcatca catcttttag tttaggatta catgtgtttt
      241 ttgtcaagaa catatttttg ttctttttca tttctgtacg ccctccctta tgccatctac
      301 acaaataatt catccgagca actccatcat ctattgaggg ttggtgaaaa aatctgtttt
      361 atccatatga tcgatttcaa tgttaaacga acgactctgg gattagtaac tattttattt
      421 ttttcttcat ttcttttttc ctctttttct cgattcagct atcttctttc atttggatat
      481 tgattatttc ttctgttgat tactatcctt ctggaattct agacgctgaa gtaattcacg
      541 ttttctttct tctagagatc ataagtaacg aatgaacgaa cttcaaataa tttttttatt
      601 gtaattcata tttgtattgt tcgatgattc ttaatacgat atgacacaat aaataaaaat
      661 aggcatggct atttacaaca atagtgctat agtagaaaac aaatgaataa ggttacttaa
      721 agaggtgaat acgatgaaac tgatcaaact atgggaagca gatttaaaga aagcttatca
      781 gctacaaact agctttggac caaatgagag tggatttatc aacaatgctt atggctatac
      841 atttgctgaa tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag gaatcgatct
      901 tcctgagagt tacgttcccg caaccgtctt tattttagaa agtgatgaac atgagtatgt
      961 aggaattttt aatttgcgcc ataaactgaa cgaaacatta agaaacggcg ctggccatat
     1021 tggttacggc atctccccta attatcgtaa aaaaggatat gccacacaag gattagcatt
     1081 agtactacaa gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg taaataaaaa
     1141 aaatcttggc agtcttaaag cacaacaaaa aaatggggct gcgatttatc gtgaagatga
     1201 gaaagaatac tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa aaggaaaggg
     1261 atttgaatgt cctttatcaa tagaacacat ttatttaatt aacttataat atagcagaca
     1321 aaattttata aacaaagaaa ccttttatct cttttcttct agaaaggata aaaggctttt
     1381 ttgtttttcc tatttgcttc tcatagctag tcagccaaga tttctatcgt atccccaacg
     1441 caatccgtgc ataacggctc atcttatctg tcgtccatgc tggataccag actagtttga
     1501 cttctgcttt cgttacttct ggcacttctt ttaatgcgtc atggatagat tctgttaaaa
     1561 tatctgctaa tggacagccc atagttgtta gagtcatctt aatgactgtt tctccatttt
     1621 caggattgaa ttctatatca taaatcaagc ctaaattgac gatgtcgatt cctaattcag
     1681 gatcaatcac catttccaag gcagcaagaa tacgttcttt gattagttct atttcttgtc
     1741 cagaccgttc ttggtttgct tcgctcatcg tacgacactc cttaacacga tttttattta
     1801 tcatactctt tttttagctg cttgtaaatg agaatttaga agtaagattc tcgtttaaat
     1861 ttataaaaat gttcttatta gatgtaatag attaaattta ttctaaaata aacctgattc
     1921 ctcatttttt ttgtgaacga ttttttattt ctggcaatac gccagttgtt cgatacattt
     1981 tttgttcgtt ttcatttgca aaccggccaa atactgaatg agttggtaaa atattgataa
     2041 atgcatcgtc gtcgatttca tgaacgatct gatcaagatc atacatttcg tagcgggtga
     2101 tgaccatcat gatcatgttc ccttgttttt ttgaatagcc accgacagac ggcagtaatg
     2161 tcattccacg taccatccgt tttgctagtt cttctgttac ctcttctggc ttgacggtaa
     2221 cgatcattgc tgttactttt tgatgactcg tgtggatggc atcaattact tgagtcattg
     2281 cataaatgga aataatcgta tacaaggcac tttcccagtt aaagaagaag cctgcgatga
     2341 ctacaatgat accatttaga atcagcatgt agtttccgac tgtttttcca gttgtttgtg
     2401 aaagtacaag agagatgata tccatcccac cagttgtaaa gcccattttt aaagataatc
     2461 ctacgccaag acctaataaa acaccgccta ctagggcatt catcaaaata ttatcagtga
     2521 tctgtccgac tggaagcagc atcgtcatca ccgatacaca tacaacattc acaaaactaa
     2581 ggatcgttgc tcgttttcct actttgagaa accctaatac aaacacaggg atattcaaaa
     2641 gaaaaataaa tgttcctgtg tctatcttga tatgaaacca ttcatataaa agcgaggaaa
     2701 tgatttgagc gatcccattc atccctgcag aaaagacatt ggctggaatc aaaaaataat
     2761 ttaaagcgac cgcagcggtc aatccggtta tcaaaataac cactattttc tttaatcttt
     2821 catttttatg atacgcagtc ataattttac tcacaagcac ctctccccta ctttggtatt
     2881 ccaaatgtaa gaataacagt ttatctatta aaataaaata cttcgaactc aaatgagaag
     2941 aattttagca aacaggtaag aatctgccct aatcctatga taagatagat gtatggtaaa
     3001 tatttatatg tatatcagga gggttcctat ggcaaaagat gctagcttcg acattgtatc
     3061 agaagtcaat acagaagaag taaaaaatgc gattcagcaa acaacaaaag aattgtccaa
     3121 tcgctttgac ttcaaggatt ctacagtaga aatcaaatta gagaacagca atctagttgt
     3181 attgagcgat gatgatttca aaatcgaaca gatcaaagat gtattatttg gtaagctgat
     3241 caaaagaaat gtgccaatca aaaatatcca tttttccgaa tcagaacacg ctctaggtgg
     3301 caaagctcga caaactgccg atttggtcaa cggaatcgat cgtgaaaacg cgaagaaaat
     3361 cactacagct atcaaaaatg aaaaactgaa agtaaaagca caaatccaag aagatcagat
     3421 tagagtgaca ggtaagaacc gtgatgatct acaagctgtg attgctttat tacgtaaact
     3481 tgacttacca atcgagttgc aattcactaa ctatcgttaa tcaaaaaacg aggctgacgt
     3541 ctgtcattcc tcgttttttt gatttactgc ttcttttgat aaccaaaaac gctgtattcc
     3601 ttcttccaat tccccaacac ttcgtacttc tcttgctgtt tgccaatgtt gtgggaataa
     3661 acgtcgataa gaaaaactag aaaatcgttg atctgccaat acaacgattc cttgatccgc
     3721 catgccacga atcactcgac caccagcttg caagactttg ttcattccag gaagctgata
     3781 agcgtaagca aatccaaggt gttccttctc atcataatat gatttgatca gttcctgctc
     3841 atgattcatc tgcggcaaac ctacacctac gatcatactg ccgatcaatc ggtcccctct
     3901 caaatcgatt ccttctgaaa aaatcccgcc aagtacgcaa aatccaatca atgtttcagc
     3961 gggatttgtt tgaaattttg ttaaaaatcg ttcgcgttct tcctcattca tgtccgtttc
     4021 ttggattagc acattgctgt cgggatattg ctgtctgaat gcttccaccg caagatctaa
     4081 atattgataa gaaggaaaga aaacgaaata attcccgacc tttccatcag caaaagcatg
     4141 aatcgtctct actaattttg ccagactgtg ttcccttttt cgataagtcg tctctaggta
     4201 atttgcgatg aaaacttgct ggtttccttc tgggaaagga ctcggcagtt tgtaagccag
     4261 acttttttgt ccccccagtg tttcctgata ataagagagt ggtgaaaaac tagcagaaaa
     4321 aagaatactg ctttttcctt tatccaacga ctgctcaaga aacaagcttg gatcaataca
     4381 aaactctttt actatcagat cgcgatacgt tttttcgact gttgtttcgt agtgatcatc
     4441 ataaaattca ctgaccttca aaaaatgaag caaatcaaaa taataaggaa gcaattgttc
     4501 ttgtgatggg tgctcaggaa attctgccag ccattcttta attttttcgc ttaattgata
     4561 acctgccttt actaaagatt cagccggtgc tttttgatga tgataatccc aatgatcttc
     4621 ttttgctatt tctcggatcg aatcaaattc ttttaatagt ttgttgaaac ggcgatgaag
     4681 ttttttgaat tcctttggta tagcttcctt acttcgtttc gttttttcat aagatagttc
     4741 tgccgaatac atctctcggg aacgattcac caaattatgt gcttcgtcaa tcaaaaacag
     4801 atagtcctcg tttttttcat cttcaaaaaa acgtcttaaa taaacagttg gatcaaataa
     4861 ataattgtaa tccccaatga ttacgtcaca ccagatactg acatctaatg atagttcgaa
     4921 aggacaaagt gtatgctttc ttgcataagt ttcgatgact tcacgagtga tctgattctc
     4981 atgattcaaa agatcccata aaccttcatt aatccgattg taataaccat tggcatatgg
     5041 gcactgatct ggattacagg tcgtctcatc taaaaagcag atcttgtctt tcgccgtcaa
     5101 agtgacactt tttacttcag ctcctgtatc ttttaatgct gtcatagcat cttcagctac
     5161 ttgtctagtt attgttttag ccgttagata aaagatcttt tctccctctt cttcccctac
     5221 agctttcaaa gcggggaata acgtagagat tgtttttcct gttccggtag gtgcttctac
     5281 aaacaatttc tgtttcgtac ggatcgtttt ataagcagct accgctagtt cacgctgacc
     5341 tttcctatac tcatcatggg ggaatttcaa agccatcaaa gaggcattgc gtacagtgcg
     5401 ccagttttct tgaaaaacaa gccattcttt ataatcttca gtcagctttt taaaaaaagt
     5461 ttctaactgc tctcttgtct gatgttcaac ctttctcgtg atcacttctt ctgccgtctg
     5521 gaagtaagtc aattgcagat ttatttcttg taaatcatgt tcttgactat agatataggc
     5581 atacaccctt gcttgatgaa agaaaagttc cacttgttct ggttctaaat cttcaaatcg
     5641 tggttcggag gttttgattt catctatata ataaataccg tctcttttga aaataccgtc
     5701 tgcacggcct tcaacgatca ggtaatattc atctaattga actgttgttt ttaaaaagac
     5761 ttctttttca tagttttctc ccgcatcttt ttggagcttg cggtggattc tcgctccttc
     5821 taatgcagta tggttacttt ttttacgatt gtcgatactt ccacgtcgca aaataaattc
     5881 tactaagtgc cgcacagata tttttgactg tttgatcatt tccaccacct ctttctctct
     5941 aagattacct gaaaaaacct agtttagcaa aaagagctct ctttccaaac ataaaaaaac
     6001 accctgctga ttaggcaggg atataggata tagaatttgg atttttggat gaatctaatg
     6061 taacaaggac atattaaaat gctatgaaaa cttactttcc ttctctttag aatatccaaa
     6121 tacttttcat atagtctgat aattcagata agtaaagaga aataagtacc tgtttttgtt
     6181 tcctagcaat gaatgcgtta tactaatgga tgaaagaagt tttggctatc aaggaggaat
     6241 gcacatgcat atcgaaagat taaaaacagg aatgattgaa gaaaactgtt atttggtcta
     6301 caacgaagaa gcacttttga tcattgaccc tggagaagat gcggaacgaa tcaaacaaga
     6361 aatcaaaaaa acaaaccaac agccggtagc gattttgctt acccatactc actacgatca
     6421 tatcggtgca gtggaacctt tacgcgcgta ttatcaaatc ccagtttatg tcagcccact
     6481 agagcaaaaa tggctaggtg atccgatttt gaatctttct ggactcggta gacatgatga
     6541 tattgctgat gttattgtct cgcctgcaga atatgaattt gaaatgaaaa cttatacttt
     6601 aggaaacatg tcttttcgcg tcgtccctac cccagggcat tctataggaa gcgtcagctt
     6661 tatttttgat gattttgtcg tttctggcga cgccctgttt agaggaagta tcggtagaac
     6721 cgatctgtat acagggaatt tagaacaatt acttcatagt attcggaccc agctgtttgt
     6781 attacctgat gaatttgcag tctatccagg acatggcgat gcaacgacga ttgaacagga
     6841 aaaaaggaca aatccttttt tcaatcgtta aaacgactta ggaggagaat aaaatgacag
     6901 aaactacact ttatatcgta cgacacggaa aaacgatgtt caatacaatt gaacgagtac
     6961 aaggctggtg tgatacaccc ctcacaaagc aaggacaaga aggcattcat tatttaggtc
     7021 gcgggttaaa aaatgtcgac ttttcatttg cttattctag tgacagtggt cgtgcaatcg
     7081 aaactgcccg tattattcta ggagaacatt ctaagggaaa agaaattcct tattttattg
     7141 aaaaaaggat tagagaatgg tgtttcgggt ctcttgaagg tggatatgac atggaaatgt
     7201 ggggtgtgat ccctcgtgta ttgaatttta aaacatatga tgaaatgttt accacagaag
     7261 tttcttttga acaaattgct aatgctgtat acaacttaga taccgctggg tgggcggaaa
     7321 cttatgaaac attgcgagac cgtgtatgga caggatttga agatatcgcc catcactgcg
     7381 aaaaaaatgg tggtggaaat gttttagtcg tatctcatgg tttgacgatc gcctttttgc
     7441 ttaatctgat cgatcctaaa cagcctgttc gtgctgggct tttaaatggc agcgtaacaa
     7501 aggtcactta tgcaaacggt cgattttcta tccacggcat caatgatacg tcttatatcg
     7561 ataacgggaa aacacatagt cgcgactcgt ttctataatg gggatatttt aatttttacc
     7621 agagaatcat aaaaggcaaa gtctgaacga gggctttgtc ttcttttttc tcttcgtgta
     7681 cttatctgct ttatttctca agtttttctt aatattaaaa aacagattgg gtttctcaaa
     7741 aaataacggt atactataag atgtatctta attgggaagg aaggaattta tgttttttgc
     7801 aaatattatc aaagcagtga ttctcggtat tattgaagga ataacggaat ggctgcccat
     7861 cagtagtact ggtcatttga tcctagcaga tgaattcatc aaactggatg ccagttcaca
     7921 atttatgtcg atgttcaatg tcgttatcca attaggggcg atcttggctg tcgtcgttct
     7981 ttattttcac aagttaaacc cattttctcc aagtaaatca acggtagaaa aaaaagatac
     8041 gtggacactt tggtttaaag tgatcgttgc ttgcatacca gccatcatca ttggtttgcc
     8101 tttggatgat tggttagaag caaatttcca caagttctta ccagttgcca ttgttttgat
     8161 cgtttacggt attgccttta ttattgtcga aaaacgcaac aaaaacaaaa cacctcgttg
     8221 gacttctctg aatgacttta cgtaccaagc agctcttctt gttgggttat tccaagtctt
     8281 ggcacttgtt cccggaacat cccgttctgg cgcaacgatc ctaggatcta tcttgattgg
     8341 tgcttcgcgt tttgtcgcta cagagttttc atttttctta gggattcctg tcatgtttgg
     8401 cgcaagcggc ttgaagatcg tcaaatattt agctgaaggg aattcattca aaatggaaga
     8461 aactgttgtt cttcttgtag gaacattcgt atcttttgtg gtttccgtct tagctatcaa
     8521 attcttacta agctatttga agaaaaacga ctttaccgct tttggctggt atcgtattat
     8581 ccttggtatc attttgattg gttattggtt tattgcaatg tagaaaaaac cagaacactt
     8641 ctct
//
LOCUS       DARDHD010000114         8414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000114 DARDHD010000000
VERSION     DARDHD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.114"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(106..1074)
                     /locus_tag="SDA06_002017"
     CDS             complement(106..1074)
                     /locus_tag="SDA06_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG4229277.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1306..2139
                     /locus_tag="SDA06_002018"
     CDS             1306..2139
                     /locus_tag="SDA06_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG4229278.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2173..3012
                     /locus_tag="SDA06_002019"
     CDS             2173..3012
                     /locus_tag="SDA06_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4229279.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3038..3598
                     /locus_tag="SDA06_002020"
     CDS             3038..3598
                     /locus_tag="SDA06_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4229280.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3797..5518
                     /locus_tag="SDA06_002021"
     CDS             3797..5518
                     /locus_tag="SDA06_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG4229281.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5683..6825
                     /locus_tag="SDA06_002022"
     CDS             5683..6825
                     /locus_tag="SDA06_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4229282.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6841..8052
                     /gene="thiI"
                     /locus_tag="SDA06_002023"
     CDS             6841..8052
                     /gene="thiI"
                     /locus_tag="SDA06_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG4229283.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2886 a         1455 c         1744 g         2329 t
ORIGIN      
        1 tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt
       61 ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa
      121 cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc
      181 tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc
      241 gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac
      301 agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc
      361 cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa
      421 gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat
      481 gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa
      541 gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt
      601 cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc
      661 ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag
      721 ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt
      781 atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga
      841 tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat
      901 gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat
      961 tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat
     1021 gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca
     1081 gacttcctta cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag
     1141 ttttgctatc agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt
     1201 aaaatggaaa agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt
     1261 ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga
     1321 ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa
     1381 tatgcagggt ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag
     1441 tcctcagttg agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc
     1501 tatcaaggaa tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg
     1561 attgcgcatt ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt
     1621 ttagtccaat tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa
     1681 cttagaaagt ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac
     1741 agcgatgaat ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc
     1801 atcatcgatg agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat
     1861 ccgaactttg ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat
     1921 aaagagtggc gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta
     1981 gcacatgaag tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat
     2041 aattctggtg gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag
     2101 ccagatggac tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa
     2161 ggggcgaagt ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat
     2221 cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt
     2281 ttatgcggtg tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg
     2341 gaattagata tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt
     2401 atcgaccacc cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac
     2461 catcgacaaa tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta
     2521 ggacaatcga tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt
     2581 ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg
     2641 ttagagatat taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag
     2701 aaaaagttag aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta
     2761 gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc
     2821 gaaattgaaa tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg
     2881 aaaggtgtag gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca
     2941 gactttgtga ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt
     3001 ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca
     3061 aaaagtagaa acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt
     3121 tacagaaaat gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt
     3181 tgcagctact tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc
     3241 attagataaa gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt
     3301 tgaacaagta gatgcactga cagatatcct ttactttact tatggttctt tttcattact
     3361 aggaatagtg cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt
     3421 tccagatgga aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg
     3481 gcagatggaa tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga
     3541 agcgattgct aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc
     3601 agagtttcac tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt
     3661 tagtgaatgc atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac
     3721 gtattatttg gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa
     3781 aacggaggaa ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat
     3841 agtcgccatt gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt
     3901 ggatgcctta gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga
     3961 tgatgttaaa aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca
     4021 aaaatggaat gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt
     4081 ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga
     4141 agctacaatg gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt
     4201 gcgggaaacg gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga
     4261 actgaaagaa aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca
     4321 aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg
     4381 agaccctgtt gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga
     4441 agctctgatg aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca
     4501 acttgaagaa atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga
     4561 agaagatctt gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa
     4621 tcttgaaaaa acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat
     4681 tgattcgtta tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa
     4741 ccaatcaacg attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat
     4801 tgagctggat cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg
     4861 aggtttccaa accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat
     4921 ccgtcaacac gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt
     4981 cttagaagat atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa
     5041 aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa
     5101 acgttttgta gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt
     5161 tgttgctact gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat
     5221 ggaagaggtc aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa
     5281 gacacatgat ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg
     5341 ttatcgtcat acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa
     5401 taaagaatat cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga
     5461 acctggtgct ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag
     5521 caaaaaagtt agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt
     5581 tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca
     5641 accgttataa tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac
     5701 agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg
     5761 attattggga atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag
     5821 caagcaagaa agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact
     5881 tcaggcggaa cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa
     5941 tatgggaatc atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa
     6001 caattaaaag aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta
     6061 cgtgtcgacg aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct
     6121 gtgaataatg aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat
     6181 tttcctaaaa ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg
     6241 caatggttga cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca
     6301 cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt
     6361 ggagggcaag aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg
     6421 gttaaagctt tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc
     6481 ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa
     6541 ggaactgact atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt
     6601 ctcgtccatg ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca
     6661 cgtaaacata tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca
     6721 tctgctgttc gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg
     6781 attattttca agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt
     6841 gtgaaataca gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga
     6901 aaaagcttta tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa
     6961 atcaaaatcc atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag
     7021 ctagttattc caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt
     7081 gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat
     7141 actgggaaag aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca
     7201 agtaatgaat tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa
     7261 gttcaaatgc gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat
     7321 ctatcgtatg aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga
     7381 atgcttatgc tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg
     7441 ggagtggaaa ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg
     7501 caaaaagcaa aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc
     7561 gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg
     7621 atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat
     7681 ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt
     7741 atggtggcta tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat
     7801 aaacttgaaa tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct
     7861 tttgaagatt gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa
     7921 aaggtccatc aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct
     7981 ggcttgaaga tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca
     8041 gaattgctat aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg
     8101 aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa
     8161 taccatttat caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat
     8221 tttatttaga gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa
     8281 tagcgaggtt gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg
     8341 cttttcaaat ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg
     8401 tgtatcagag gttg
//
LOCUS       DARDHD010000115         8401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000115 DARDHD010000000
VERSION     DARDHD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.115"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(49..546)
                     /locus_tag="SDA06_002024"
     CDS             complement(49..546)
                     /locus_tag="SDA06_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG4229284.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(676..1386)
                     /gene="budA"
                     /locus_tag="SDA06_002025"
     CDS             complement(676..1386)
                     /gene="budA"
                     /locus_tag="SDA06_002025"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG4229285.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1399..3063)
                     /gene="alsS"
                     /locus_tag="SDA06_002026"
     CDS             complement(1399..3063)
                     /gene="alsS"
                     /locus_tag="SDA06_002026"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG4229286.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3271..3933)
                     /locus_tag="SDA06_002027"
     CDS             complement(3271..3933)
                     /locus_tag="SDA06_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4229287.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3943..4758)
                     /locus_tag="SDA06_002028"
     CDS             complement(3943..4758)
                     /locus_tag="SDA06_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4229288.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5019..5462)
                     /locus_tag="SDA06_002029"
     CDS             complement(5019..5462)
                     /locus_tag="SDA06_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG4229289.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5596..5934)
                     /locus_tag="SDA06_002030"
                     /pseudo
     CDS             complement(5596..5934)
                     /locus_tag="SDA06_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5922..6299)
                     /locus_tag="SDA06_002031"
     CDS             complement(5922..6299)
                     /locus_tag="SDA06_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4229290.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6523..7716
                     /locus_tag="SDA06_002032"
     CDS             6523..7716
                     /locus_tag="SDA06_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4229291.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7882..8304)
                     /locus_tag="SDA06_002033"
     CDS             complement(7882..8304)
                     /locus_tag="SDA06_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229292.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2467 a         1693 c         1419 g         2822 t
ORIGIN      
        1 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
       61 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
      121 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
      181 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
      241 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
      301 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
      361 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
      421 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
      481 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
      541 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
      601 taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
      661 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
      721 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
      781 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
      841 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
      901 cgtaaagaac ccgactaatg ttccttggat attgctttgt tggaattctg gttgagactc
      961 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     1021 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     1081 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     1141 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     1201 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     1261 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     1321 tccatccatc aagccggcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     1381 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     1441 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     1501 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     1561 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     1621 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     1681 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     1741 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     1801 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     1861 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     1921 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     1981 tccttttgat tgaacaatgt cccgctccgt taattttgct tgaagagatg ctaaatattc
     2041 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     2101 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     2161 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     2221 gattggatca tagccaattg caataactag atcacttcgt ttcaaaagca tatctcctgg
     2281 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     2341 tccagctgct tggaatgtct ctacaacagg caatgctgtc ttttcaacta attgcctgat
     2401 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     2461 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     2521 atcgctcaat ggcttgatga cattaccttg gacaggtgca tccactacat cttgcgggat
     2581 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     2641 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     2701 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     2761 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     2821 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     2881 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     2941 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     3001 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcagc
     3061 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     3121 aagtgataca gtttctaaac ctgtttcata caaaagtata gcacgtttta tttcatttgt
     3181 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa aaaactgtca
     3241 ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca tcaatttacg
     3301 attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac accattgggg
     3361 ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta aactcacata
     3421 ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt tcgacgctgg
     3481 atttctcacc tgaaaatacc agcagttggt gctaaaggaa actgataaac aagcaagtaa
     3541 aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga aacaatcaaa
     3601 aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct ttagccaaaa
     3661 ttggcgaaac atctttatga acaaacgtac tggtgaaaaa ctattttctt ttgctaattc
     3721 ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa
     3781 ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat acaagatatt
     3841 gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa agaattccat
     3901 ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct tcattcctga
     3961 tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag caacaggaag
     4021 aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata gcccaataaa
     4081 cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa tgaatggccc
     4141 catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa tcgccggttt
     4201 tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat cgatccgcgc
     4261 actctcacat aaagaatctg ggatactact gtaatattgc ttcaacaaga acacaccaaa
     4321 agcattgaac gcttgtaata gcaccaacga ccacaacgta tctgttaaac ccacttttct
     4381 catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg tgccgatata
     4441 tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt aagcagcaaa
     4501 actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa
     4561 aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat gccaagtttt
     4621 agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg aagcagaaac
     4681 catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa tcatacccaa
     4741 ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt catttcgaac
     4801 acctccaaag ttataatctt catttctttg taatgattac aatccagaac aacggttatt
     4861 atagctgatt catttccgtt cttcttattt ttgtaagcgc ttatttaaca agttatgtaa
     4921 atctctttat ttttttgatt taaaaatgaa aaaatacaaa agcaagactg tcatacgtct
     4981 cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa agcttctttg
     5041 aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt ccagactagc
     5101 atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt tgaatgatga
     5161 cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag cgcaaataca
     5221 ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat gcctaaccag
     5281 tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac attcgcaaag
     5341 tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat gagcaatccc
     5401 catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac tggatctagc
     5461 aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc acttgttccg
     5521 ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag taatagtgcc
     5581 aatgtattcg aactatcagc ttccacacag ccgatcgtaa atcggccgct gaataattgt
     5641 ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat ttcttctgct
     5701 cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa
     5761 cagttctgtt cctagttact cttctaactc cttcagttgt cggcttaagg ttggttgtgt
     5821 aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata
     5881 ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt
     5941 ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacat
     6001 tcttgcattt cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc
     6061 cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg
     6121 atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct
     6181 gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca
     6241 taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc
     6301 tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac
     6361 tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc
     6421 ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata
     6481 cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga
     6541 aaggtattag tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta
     6601 gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc
     6661 atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct
     6721 gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct
     6781 ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt
     6841 gtaggagcgg gaggagaata tggggcgtgt atgtccttga tttccgaaag tttttcaaaa
     6901 aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg
     6961 gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta
     7021 ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa
     7081 ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa
     7141 catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat
     7201 tttgggttaa tgaattggct accttcaatt atgcaaaatc gattagggct ttcggtttct
     7261 gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt
     7321 ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca
     7381 tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct
     7441 gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt
     7501 ttgtatccaa cagaaatccg cgcgacggcg aataatttcg ttatgaacct tggacgagct
     7561 gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc
     7621 gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact
     7681 ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga
     7741 aagaggtgtg gaaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag
     7801 acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa
     7861 tttccttcta tctggcggcg actattcttt gacgtccgtc atttcaaagt actttactaa
     7921 taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc
     7981 caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct
     8041 gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg
     8101 ccgattattc tcattcttca aaatgacctt atcaggatat tgttggaaca attcttctaa
     8161 atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc
     8221 tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga
     8281 aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat
     8341 acaagaaaaa aacggataca aatatcatat tgtcacaccg tcaatagtgc ataaacctgt
     8401 t
//
LOCUS       DARDHD010000116         8174 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000116 DARDHD010000000
VERSION     DARDHD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.116"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SDA06_002034"
     CDS             94..1248
                     /locus_tag="SDA06_002034"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG4229293.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SDA06_002035"
     CDS             complement(1270..2022)
                     /locus_tag="SDA06_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4229294.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SDA06_002036"
     CDS             complement(2386..5052)
                     /locus_tag="SDA06_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4229295.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SDA06_002037"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SDA06_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4229296.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SDA06_002038"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SDA06_002038"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG4229297.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2374 a         1697 c         1457 g         2646 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aata
//
LOCUS       DARDHD010000117         8091 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000117 DARDHD010000000
VERSION     DARDHD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.117"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SDA06_002039"
     CDS             476..1339
                     /locus_tag="SDA06_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4229298.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SDA06_002040"
     CDS             complement(1468..3090)
                     /locus_tag="SDA06_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4229299.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SDA06_002041"
     CDS             3339..3860
                     /locus_tag="SDA06_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4229300.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SDA06_002042"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SDA06_002042"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG4229301.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SDA06_002043"
     CDS             5941..6759
                     /locus_tag="SDA06_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG4229302.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SDA06_002044"
     CDS             complement(6862..7059)
                     /locus_tag="SDA06_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229303.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SDA06_002045"
     CDS             7228..7806
                     /locus_tag="SDA06_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4229304.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SDA06_002046"
     CDS             7901..8038
                     /locus_tag="SDA06_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229305.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2417 a         1472 c         1597 g         2605 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag c
//
LOCUS       DARDHD010000118         8057 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000118 DARDHD010000000
VERSION     DARDHD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.118"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..538)
                     /locus_tag="SDA06_002047"
     CDS             complement(17..538)
                     /locus_tag="SDA06_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4229306.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="SDA06_002048"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="SDA06_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG4229307.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="SDA06_002049"
     CDS             4618..5505
                     /locus_tag="SDA06_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG4229308.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="SDA06_002050"
     CDS             5502..6671
                     /locus_tag="SDA06_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229309.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="SDA06_002051"
     CDS             6695..8050
                     /locus_tag="SDA06_002051"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG4229310.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2585 a         1490 c         1829 g         2153 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatat
//
LOCUS       DARDHD010000119         8032 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000119 DARDHD010000000
VERSION     DARDHD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.119"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(47..895)
                     /locus_tag="SDA06_002052"
     CDS             complement(47..895)
                     /locus_tag="SDA06_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4229311.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="SDA06_002053"
     CDS             complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="SDA06_002053"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG4229312.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3396..3575)
                     /locus_tag="SDA06_002054"
     CDS             complement(3396..3575)
                     /locus_tag="SDA06_002054"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG4229313.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="SDA06_002055"
     CDS             complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="SDA06_002055"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4229314.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5723..8002
                     /locus_tag="SDA06_002056"
     CDS             5723..8002
                     /locus_tag="SDA06_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229315.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2355 a         1707 c         1452 g         2518 t
ORIGIN      
        1 tacgttttgt tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca
       61 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
      121 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      181 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      241 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      301 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      361 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      421 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      481 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      541 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      601 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      661 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      721 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      781 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      841 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      901 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      961 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
     1021 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1081 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1141 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1201 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1261 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1321 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1381 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1441 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1501 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1561 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1621 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1681 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1741 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1801 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1861 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1921 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1981 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     2041 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2101 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2161 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2221 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2281 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2341 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2401 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2461 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2521 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2581 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2641 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2701 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2761 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2821 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2881 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2941 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     3001 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3061 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3121 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3181 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3241 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3301 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3361 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3421 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3481 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3541 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3601 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3661 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3721 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3781 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3841 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3901 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3961 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     4021 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4081 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4141 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4201 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4261 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4321 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4381 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4441 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4501 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4561 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4621 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4681 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4741 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4801 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4861 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4921 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4981 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     5041 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5101 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5161 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5221 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5281 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5341 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5401 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5461 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5521 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5581 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5641 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5701 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5761 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5821 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5881 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5941 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     6001 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6061 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6121 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6181 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6241 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6301 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6361 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6421 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6481 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6541 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6601 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6661 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6721 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6781 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6841 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6901 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6961 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     7021 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7081 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7141 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7201 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7261 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7321 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7381 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7441 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7501 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7561 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7621 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7681 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7741 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7801 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7861 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7921 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7981 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt ta
//
LOCUS       DARDHD010000120         7974 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000120 DARDHD010000000
VERSION     DARDHD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.120"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..395
                     /locus_tag="SDA06_002057"
     CDS             207..395
                     /locus_tag="SDA06_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG4229316.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            473..1486
                     /locus_tag="SDA06_002058"
     CDS             473..1486
                     /locus_tag="SDA06_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4229317.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1553..2587)
                     /locus_tag="SDA06_002059"
     CDS             complement(1553..2587)
                     /locus_tag="SDA06_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229318.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2702..4927
                     /locus_tag="SDA06_002060"
     CDS             2702..4927
                     /locus_tag="SDA06_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4229319.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            4945..6828
                     /locus_tag="SDA06_002061"
     CDS             4945..6828
                     /locus_tag="SDA06_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG4229320.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6809..7783
                     /locus_tag="SDA06_002062"
     CDS             6809..7783
                     /locus_tag="SDA06_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG4229321.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2589 a         1454 c         1686 g         2245 t
ORIGIN      
        1 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
       61 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      121 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      181 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      241 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      301 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      361 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      421 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      481 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      541 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      601 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      661 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      721 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      781 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
      841 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
      901 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
      961 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1021 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1081 tagtctgaaa ggaaccattt cttattgggg acctgttgat ttgaagctaa cagcacagca
     1141 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1201 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1261 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1321 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1381 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1441 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1501 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1561 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1621 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1681 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1741 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1801 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     1861 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     1921 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     1981 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2041 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2101 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2161 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2221 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2281 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2341 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2401 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2461 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2521 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2581 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2641 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2701 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2761 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     2821 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     2881 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     2941 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3001 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3061 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3121 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3181 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3241 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3301 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3361 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3421 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3481 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3541 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3601 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3661 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3721 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3781 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     3841 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     3901 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     3961 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4021 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4081 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4141 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4201 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4261 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4321 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4381 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4441 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4501 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4561 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4621 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4681 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4741 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4801 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     4861 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     4921 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     4981 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5041 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5101 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5161 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5221 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5281 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5341 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5401 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5461 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5521 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5581 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5641 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5701 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5761 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     5821 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     5881 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     5941 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6001 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6061 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6121 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6181 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6241 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6301 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6361 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6421 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6481 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6541 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6601 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6661 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6721 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6781 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     6841 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     6901 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     6961 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7021 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7081 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7141 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7201 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7261 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7321 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7381 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7441 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7501 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7561 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7621 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7681 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7741 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7801 tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg aacaagagag
     7861 taaaaaagtt ctcagatact tttgttcatg actattttct tcaataaaac caattgttat
     7921 ttttctgaaa aacaaagtta actatcagaa aattaaaaaa agtttcataa aaat
//
LOCUS       DARDHD010000121         7919 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000121 DARDHD010000000
VERSION     DARDHD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.121"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            81..1172
                     /gene="phnW"
                     /locus_tag="SDA06_002063"
     CDS             81..1172
                     /gene="phnW"
                     /locus_tag="SDA06_002063"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG4229322.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1165..1293
                     /locus_tag="SDA06_002064"
     CDS             1165..1293
                     /locus_tag="SDA06_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4229323.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1297..1842
                     /locus_tag="SDA06_002065"
     CDS             1297..1842
                     /locus_tag="SDA06_002065"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4229324.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2296..3987)
                     /locus_tag="SDA06_002066"
     CDS             complement(2296..3987)
                     /locus_tag="SDA06_002066"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG4229325.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4407..5354)
                     /gene="arcC"
                     /locus_tag="SDA06_002067"
     CDS             complement(4407..5354)
                     /gene="arcC"
                     /locus_tag="SDA06_002067"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG4229326.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5469..6488)
                     /gene="argF"
                     /locus_tag="SDA06_002068"
     CDS             complement(5469..6488)
                     /gene="argF"
                     /locus_tag="SDA06_002068"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG4229327.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6579..7808)
                     /gene="arcA"
                     /locus_tag="SDA06_002069"
     CDS             complement(6579..7808)
                     /gene="arcA"
                     /locus_tag="SDA06_002069"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG4229328.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2250 a         1616 c         1464 g         2589 t
ORIGIN      
        1 aatcatcatt tatattggat gttaacaaga gcagcttctt gctctttgtg tatttcaaaa
       61 aagaaatgaa aggaaatcat atgtataaat tattaacttc tggaccattg acgacttctg
      121 aaacagtgaa aaatgctatg ctgatcgatc attgtacttg ggatcaagaa tacaaagaaa
      181 ttacacaatg gataagaaaa aaattattgg atcttgctca agtatcggaa ggaatataca
      241 caacggtctt aatgcaaggt agcagctcct tcggggtaga atcggtatta agttctgtga
      301 tcaggccaga agatactgta ctgatttgtt caaacggcgc atatagacaa cgaatgatca
      361 acatgtgcga atgtctgaaa ctgaactata ccgtttatga ggtatcggaa catcaaatcc
      421 tccaagctca tgtgattaca cagctgattg aagagaatcc gtctatctcc gcactagcaa
      481 tggtccatag cgagactact tccggtattt tgaataattt ggaagagatt gctgctgtca
      541 cacaagaaag agatcttttg tttatagtgg acgcgatgtc aagctttggt gggataccga
      601 tttgtgtagc cgaactaggg attgattttt tgatttccag ctcaaacaaa tgtattcaag
      661 gtgttccagg cttttctttt gtacttgcta ataaagcgga actggaaaaa cgaaaaaatg
      721 ttagccgaag tttgtcactt gatctatatg accagtaccg cgtgatggaa aacgagaatg
      781 gcaaatggcg tttcacttcc cctacccata cggttttggc ttttcaacaa gcgctgaagg
      841 aactagaggc agaaggagga atcgaaactc gatattcacg ttatctcgcc aataatcatg
      901 tgctacgaca aaaaatgaca gagatggggt tccagccttt cattcaagaa tatcaggggc
      961 cattcatcac ctcttttctt taccctaagg actggcgatt tgattttaat catttttatc
     1021 agtatttaaa gcaaaatgga tttgtgatct atcccgggaa aatcagcgat catgaatgtt
     1081 ttcgtatcgg aaatattggc gagatctatc cggaagatat tttggaactg acagcaatta
     1141 tcaaagacta taaggagaac gttaatgatt aaaactgtaa tttttgattg ggcaggaaca
     1201 actgtggact ttggctgtat ggctcctgtc cacgctttta gaaatgcttt tctagaaaaa
     1261 ggaatccaac tgactgacaa agaaatccgc taaccgatgg gaaaattaaa atgggatcat
     1321 atccaacagc ttctttccct cccttctgtc aaaaaacaat ggatagctat ttatggacaa
     1381 ctacctcagg aaaaagaggt ggaggaattg tatcaacaat ttgaacatta tctgtttaaa
     1441 gatttggtca agcattctac cttgaaaccc gacacattgg aaacgatcga acggttgaga
     1501 aacgctggga tcaatattgg ttccacgaat ggatttaatg ctcctatgat gacaatcgta
     1561 gcagaaaccg caaaaaaaga aaggtattca ccagacttca tggcaacttt tgaagacgtc
     1621 gaagggtatg gtcgaccgtt tccttatatg atccataaaa acatgcagta cttcaaaaac
     1681 aagtcggttg atgaagtaat caaagtcgga gatacggtct cagatatcca ggaagggaaa
     1741 aatgccggag tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg gatttcttat
     1801 gatgagtacc aaaaattaga gatggggaaa aagatcgttt aaaacagtct gttgctctta
     1861 gattccaaca agccggcgct gatatttgtg taaacaattt aaaagagtta acagaataca
     1921 ttttgtacgc tgacatgaat ccagaatgac ttttatacaa aataagaggt tgtgaaaaag
     1981 ctgtcctgta tcaagtaata aaaacagcca agcaaaaata gctcctcata tttttcgttt
     2041 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaaagg
     2101 gagctgtgac acgacttttg tcacagtccc cttatttgca aacaaacagt gtttaagaga
     2161 caacctctcc actaaacggt gttcattcag ctgactctcg gtatttgctc aaaaaacgat
     2221 aaaatttgaa aagctatttt ctcttttgtt tctctaatac tcggatcaga acgctgattt
     2281 cacattctct ttttcttaca tcttatctgg tgcatgaagt cctagcaaac gaaggtcttc
     2341 tttcaataat acagtcactg cataaactag agctaagcgt gcttcttttt gttcatcatc
     2401 agctaaaatc ttcgtatgag catagtattt gttgaatgct tgtgctaatt tgattgcatg
     2461 tttcgcaatc actgacggtt cgtatttttc cccagcagac aagaccgttt cagggtattt
     2521 ttgaactaat ttaaccactt cccagctggt atcgtcattc aatgcatagt ctgctgtttc
     2581 tgaagggaca aatcctgctt tttccaacag gctgactgca cgagcatgtg tatattgcac
     2641 ataaggtcca gtttctcctt caaatcgaac aacttcttcc aggttaaagt caaatgtatt
     2701 cagtcgatcg tttttcaaat cgtggaaaat gactgcccca accccaactt gtttagctac
     2761 agcatctttg ttttccaaat cagggttttt ctcgctgatt tgttctttag cagattggat
     2821 tgcttcattt aatacttctt ctaataagac gatctttcct ttacgagttg ataatttttt
     2881 accaccttgc gtgatcaaac caaatggaat atggtgcatg tcgtcagacc agtcaaagcc
     2941 catttccttc aagacagctt tcaactgttt aaaatgatag ctttgttcat tccctactac
     3001 atatagagat tgtttgaagt cgtaagtacg tttacggtac aatgcagcag ctaagtcacg
     3061 tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac gctggattca ggtcataagc
     3121 agataggtca acgatctctg cacctttatc ctcgttcagc aaatgttttt cttctaaaag
     3181 tttgacgatt tcatccatct tgtcattgta gaaagcttca ccgttcaacg aatcgaaacg
     3241 tacttccagc agatcataga ttttattgaa ttctttcatc gattcatcac ggaaccattg
     3301 ccataattga atagcctctt tatcgccttc ttccagacgt ttgaaccaag cacgagcttc
     3361 ttcatttaat tctggttctg tttcagctac ttcatggaat tggacgtata aacgaagcaa
     3421 ttcattgatt ggttctgctt taacagcttc ttctgtaccc cattttttgt aagcgacgat
     3481 cagttttcca aactgtgttc cccagtcacc taaatggttg atgcggattg gctgataacc
     3541 gattttttcc ataatgaaac cgatcgaatt accgataact gtcgaacgca aatgtcccat
     3601 tgagattggt ttggcaatat taggagaaga catgtcgatt ggtactgttc cttgattccc
     3661 gatgttgcta tccccataat gttctttttc tttcactact gtttgcaaaa cttttttgct
     3721 gataagttct ttattcatga aaaagttcaa gtaaggtccg acaacttcga ttttttcaaa
     3781 gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg cttttcggta
     3841 aacttttgct aatgaaaagg ctggaaatgc aacatctccg tgttcagctg attttggatt
     3901 ttctagtaat tgctcgactt gttccagtgt taaatcatct ttgacagcgt catagacagc
     3961 ttttgcgaca agatctttat tattcatctt tattcctcct aaagtgttcc cttgattgtt
     4021 aaacaaaaaa aatctctagc gacggcatac ttagtatgcc atcactagag acgagatttc
     4081 ccgcggtacc actccaattg tcctaaaaaa ggaccagctc catctgatat cggtagacga
     4141 ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg tatcttcttc attcggcttc
     4201 caccacttcc gaattcgcta gccaagaatg atacaagtac tctcttgttc aacaaatctt
     4261 agtcttatta aactttcttg aattatatca gaaaaaaaaa cgaaaggcta gtcgtttcat
     4321 aaaaaaacaa tccagatggc ctaaaggaga agaaatacca cctggatagc tataaatcat
     4381 ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc ttcttcagaa
     4441 gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt tggttttgct
     4501 tcaacaaatg caatagctgc ttctactttc ggaagcatgc ttcctggagc gaattgcttt
     4561 tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt ttgatctggt
     4621 ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa atccgcatcg
     4681 atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc tcgtccttct
     4741 aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac tgtgataact
     4801 ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg tttaggggaa
     4861 gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt ggagtcagca
     4921 ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt acttggattt
     4981 ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac cacatcttta
     5041 tcgatccctt tttccttcag tacttcgccc atggcgtttt gtaaccagta accgatagat
     5101 ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc agaatcagca
     5161 gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga gatgatcaat
     5221 tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa cgcttcttgt
     5281 tgggcttttg cacttgcatc cgtagacaag atggcattgc cccctaaagc gacaactact
     5341 tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct agataacaaa
     5401 acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg agatgaatgt
     5461 ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat gatcgctttg
     5521 attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct gcggaagact
     5581 tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc accgtaaaca
     5641 gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt
     5701 gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat acgttcttcg
     5761 aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc tgctccgtct
     5821 acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc agcaaagcct
     5881 tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac gatacgcata
     5941 ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt tcgaccatct
     6001 ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat agttaaaaag
     6061 tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac tggaacacca
     6121 gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc gattccgtca
     6181 aacatacgtc ctagtacttt agctgtatct tctgtggatt cttttttacc taattggata
     6241 tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt agtaaatgca
     6301 gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc ttctagatag
     6361 tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat
     6421 tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg gaatactgat
     6481 tctttcatat taaacattct cctttagatt gtttttattt aagattaaag aggaaatccc
     6541 gacggcagct gacagtaaga ctgccgtcag gaaacaaatt acttcaagtc ttcacggaca
     6601 agtggttggc tcatgcagcg tggaccacca cgacctcgtg acaattcact tgaattgatt
     6661 tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt tcggttataa
     6721 gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca ttgttctcga
     6781 ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc taatccaaga
     6841 gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc atcaccatct
     6901 ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg atggatagtg
     6961 aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc acggttgttc
     7021 ggaattttga ttgccaacac tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct
     7081 aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac tactttatcg
     7141 cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc caaccatact
     7201 tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt aaacatcgat
     7261 tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc cattgatgcg
     7321 gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg atagtcatcg
     7381 tctgctgaca gatcatatag actgctagaa cgtacttcga tatcttttgt acgaacccca
     7441 gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc atgaagaccc
     7501 tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa gaattgttct
     7561 ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag agtctcgacg
     7621 ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt cttgagcaat cggaagatat
     7681 ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc cacttcttgt
     7741 ccaggccgtt tcagtaaaac tgtctttaat ttccctattt cagagaaaac gtgaataggt
     7801 ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc ctccttttgt
     7861 aatcactttc ataattttgt ctcattcaaa atgagacatt cctcacatta atattcgat
//
LOCUS       DARDHD010000122         7882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000122 DARDHD010000000
VERSION     DARDHD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.122"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(22..996)
                     /locus_tag="SDA06_002070"
     CDS             complement(22..996)
                     /locus_tag="SDA06_002070"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4229329.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="SDA06_002071"
     CDS             complement(1076..1951)
                     /locus_tag="SDA06_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG4229330.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="SDA06_002072"
     CDS             2081..3988
                     /locus_tag="SDA06_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG4229331.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="SDA06_002073"
     CDS             complement(4529..7807)
                     /locus_tag="SDA06_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG4229332.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2225 a         1664 c         1463 g         2530 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aa
//
LOCUS       DARDHD010000123         7788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000123 DARDHD010000000
VERSION     DARDHD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.123"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            40..741
                     /locus_tag="SDA06_002074"
     CDS             40..741
                     /locus_tag="SDA06_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4229333.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1350..2366)
                     /gene="tsaD"
                     /locus_tag="SDA06_002075"
     CDS             complement(1350..2366)
                     /gene="tsaD"
                     /locus_tag="SDA06_002075"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG4229334.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2363..2827)
                     /gene="rimI"
                     /locus_tag="SDA06_002076"
     CDS             complement(2363..2827)
                     /gene="rimI"
                     /locus_tag="SDA06_002076"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4229335.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2834..3376)
                     /gene="rimI"
                     /locus_tag="SDA06_002077"
     CDS             complement(2834..3376)
                     /gene="rimI"
                     /locus_tag="SDA06_002077"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4229336.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3360..4082)
                     /gene="tsaB"
                     /locus_tag="SDA06_002078"
     CDS             complement(3360..4082)
                     /gene="tsaB"
                     /locus_tag="SDA06_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG4229337.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4273..5253)
                     /locus_tag="SDA06_002079"
     CDS             complement(4273..5253)
                     /locus_tag="SDA06_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229338.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5277..6761)
                     /gene="galT"
                     /locus_tag="SDA06_002080"
     CDS             complement(5277..6761)
                     /gene="galT"
                     /locus_tag="SDA06_002080"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG4229339.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6773..7762)
                     /gene="galE"
                     /locus_tag="SDA06_002081"
     CDS             complement(6773..7762)
                     /gene="galE"
                     /locus_tag="SDA06_002081"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG4229340.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2174 a         1690 c         1385 g         2539 t
ORIGIN      
        1 gacaataaat atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat
       61 tttcactttt caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc
      121 caacagatca aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac
      181 gatggtgatc ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt
      241 tatgatgaaa gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct
      301 atgtcaggaa tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt
      361 gaaacattct accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag
      421 ttgatctata tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa
      481 atcggattga cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa
      541 gaattgggcg taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa
      601 gaattagcag tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta
      661 cgtgattccg ggctgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact
      721 actccagcta tgagagaata acgcgaacaa aaataagcag tgaccctgat aaatatcagg
      781 gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta gatcactgga
      841 caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa
      901 tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt attcgatatt
      961 cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat gataaataag
     1021 ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata gggctttgac
     1081 aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact cttcggtcac
     1141 aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga
     1201 gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt
     1261 aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact
     1321 tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct tggatcagca
     1381 ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc
     1441 attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt
     1501 ttagctgcca aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca
     1561 gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat
     1621 acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca
     1681 cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa
     1741 tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt tccctgatga
     1801 gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg
     1861 tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct
     1921 tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac
     1981 tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata
     2041 agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag
     2101 ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca
     2161 gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct
     2221 acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta
     2281 gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca
     2341 atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa
     2401 tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact
     2461 tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat
     2521 gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt
     2581 ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg
     2641 agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa tcagagtgag
     2701 gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat
     2761 tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc
     2821 aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg
     2881 tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc cgataaagtc
     2941 tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta
     3001 tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg
     3061 gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac
     3121 caatcattct tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt
     3181 ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat
     3241 ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt
     3301 gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc
     3361 taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct
     3421 gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcaact
     3481 aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg aactgtatta
     3541 atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca atcactcccg
     3601 acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata
     3661 tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc cccagcgtat
     3721 acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc gatgcaatta
     3781 gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc
     3841 gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc
     3901 gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca
     3961 ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt
     4021 tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc
     4081 atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt
     4141 ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat ctgctggcgg
     4201 gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat tttcccacag
     4261 cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca caataatctt
     4321 tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg ctagctctcc
     4381 catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt tcgctacact
     4441 gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt tttcttgaat
     4501 cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat caggaacatg
     4561 cggattcgtt tttaaatatt gcttcactgc ttgatatcct gactcgacag aaaaatctgc
     4621 ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa atgccataat
     4681 tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag caagcatagc
     4741 gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac ttgacttaaa
     4801 gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca aaagcaaggc
     4861 attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta gtgcgatcgt
     4921 tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag taacgtttgc
     4981 ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat cttcaagctc
     5041 ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt
     5101 acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct ttgtggctaa
     5161 gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag gagaaactcc
     5221 tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt ttacgtttat
     5281 aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt gaatactcca
     5341 gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac atcggtgacc
     5401 gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg acgatgatat
     5461 tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag ttctggtttc
     5521 aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt ctcctttttg
     5581 atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc tgaaacattg
     5641 ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc aattggcgta
     5701 atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt ttcatccgaa
     5761 tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt tttctctgtt
     5821 gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac ttgcggataa
     5881 tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc atgacgtcct
     5941 gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc agcattcgaa
     6001 ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa tgtttccttt
     6061 gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt gtaataagca
     6121 taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat aatccggtga
     6181 ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat gcaaagcatg
     6241 caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt tggatctttt
     6301 tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc tgccggaaat
     6361 ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag
     6421 aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg
     6481 actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct
     6541 ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc caataagtct
     6601 acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc
     6661 cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc
     6721 gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt
     6781 cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc gctttgatat
     6841 ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa gcgactaatc
     6901 ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt
     6961 ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga
     7021 agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat
     7081 cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat
     7141 aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg
     7201 tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt
     7261 agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca
     7321 tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg
     7381 gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt ttattcacgt
     7441 tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca
     7501 tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt
     7561 caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat
     7621 aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag
     7681 ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc
     7741 cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaa
//
LOCUS       DARDHD010000124         7758 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000124 DARDHD010000000
VERSION     DARDHD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.124"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            109..2436
                     /locus_tag="SDA06_002082"
     CDS             109..2436
                     /locus_tag="SDA06_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4229341.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2562..2891
                     /locus_tag="SDA06_002083"
     CDS             2562..2891
                     /locus_tag="SDA06_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4229342.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2931..3935
                     /locus_tag="SDA06_002084"
     CDS             2931..3935
                     /locus_tag="SDA06_002084"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG4229343.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3932..4378
                     /locus_tag="SDA06_002085"
     CDS             3932..4378
                     /locus_tag="SDA06_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4229344.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4528..5343
                     /locus_tag="SDA06_002086"
     CDS             4528..5343
                     /locus_tag="SDA06_002086"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4229345.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5549..6721
                     /locus_tag="SDA06_002087"
     CDS             5549..6721
                     /locus_tag="SDA06_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4229346.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6883..7377)
                     /locus_tag="SDA06_002088"
     CDS             complement(6883..7377)
                     /locus_tag="SDA06_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229347.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2626 a         1461 c         1538 g         2133 t
ORIGIN      
        1 aaaaagagaa aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt
       61 agaataacgt gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt
      121 aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa
      181 gctgtatcta aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact
      241 ttcttaggga gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct
      301 aaccaaacga cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc
      361 accaatcagt ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat
      421 ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca
      481 ggtagaagcc agacatttag tactgttgcg gccactgcga atttcaacgg acataccata
      541 ccagatggat atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg
      601 atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac
      661 cgtccagata tccataatct gtttattggc tccacagtaa atggtactac tatcacattc
      721 ccaaaaagta tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc
      781 aatgcggata aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga
      841 ggtatgagta ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac
      901 tcgatcaaaa atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat
      961 caagcagcaa cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat
     1021 ggtggagcag caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac
     1081 aaagtggtta tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct
     1141 ttcacagccc tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc
     1201 actattaagt atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt
     1261 acttacggaa gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc
     1321 tttacagctg tttcagcgat agaaacaaag aaccaatcag caatgagtgt tttagctagt
     1381 ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc
     1441 atcctatatt ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat
     1501 gatggtcttt ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca
     1561 tcccttttaa acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa
     1621 gcgatgaatg aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct
     1681 tggcaattga caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact
     1741 atcgtgcaga catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt
     1801 ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag
     1861 ggtgtcacta gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca
     1921 gatgcacaat cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat
     1981 agcggaatag acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac
     2041 acatcaggaa cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc
     2101 tttaattatg atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt
     2161 gtcttaggcg aatcattgga ttatacccta acagttaaca gccggacaga tattggatca
     2221 caaatagtaa cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca
     2281 acagataccg ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata
     2341 ggtctactgc cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt
     2401 atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg
     2461 atatatttgc tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt
     2521 cagctagaga atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga
     2581 tgccaagtat cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag
     2641 ggttgaacgg cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt
     2701 cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa
     2761 aaatactggc agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta
     2821 gtgcatcgga caaagatgag caaggtcgtg acgctgttta ccgatttacg gaaataaaga
     2881 cttcagatta agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt
     2941 catctaatca taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg
     3001 ctgcactcac attaacaaaa acagtagaga ataagcagac agattttgca gatggggata
     3061 aagtccctta tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata
     3121 cgatcaaaga tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga
     3181 ttggtggaga caagattcat acatttcaca cacaaaaaat aaaacatggt tttgtcttat
     3241 cagattctgg aaaagatctt agttcgtttg ctggaaaaaa attaacgatc cgttaccaaa
     3301 tgacattgaa agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg
     3361 acaatcaaac agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag
     3421 tggatcggca aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc
     3481 aatacttagc gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa
     3541 cgatttttac ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt
     3601 acgaactagt ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct
     3661 ttgaagttga aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg
     3721 aaccaaaagc aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc
     3781 catcaaatga aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt
     3841 tctcaatttt agggttattg ctagtccttt caacagggac agcttggttt tataaaaaac
     3901 aacaaaaagg aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac
     3961 gtttttagga actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt
     4021 ttctgcagcc gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt
     4081 cacttcagca ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga
     4141 accgttgaac ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa
     4201 agacacagca gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg
     4261 attagctaac ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt
     4321 agaaacacca aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctagac
     4381 cctgacaaac ctttagacac gattcaccta tatgctaaaa atgtcatcaa agaaagcaat
     4441 atcaaaatcc agaaagctgg gaacttttca ggggtagata agttagcagg ggcgcaattc
     4501 cagatcaaaa attcagagaa tcaattcgtg accggttttg atgaaaacac tggtgtcgct
     4561 ctctatgatc ctgctgagtc agcgttgaca ttcacaactg atactgaagg atccatcagt
     4621 gtcaatggat taatagacgg caattatact ttagttgaaa caaaagcacc agatggttat
     4681 caattaacca atccacaaaa agaaactact tttatagtaa aaaacggaaa attgcaacaa
     4741 acaaccggta tcatccatgg cgcagatgaa gccaatcttg ggaatccaaa cggtgtagtc
     4801 aatcgggaaa atacaactac ggataacacg attactgtag aaaatgtcca aaactacggt
     4861 gatttccact tcatcaaaca agatgtgaac acgcaagaaa aattagccga tgcagaattt
     4921 tctattctaa gagcaaaaga tccaactgtt aaattaatga aaaaaatagc aaaccttgcc
     4981 tcaggagaat acccatatgc ttggtcagat gaagtcacag gttctgactg ggaagaagtg
     5041 aaattgattt ctgattcaga agggaaattt tcaatcgaag ggctgaaaca aggggcatac
     5101 tgccttcaag aaacaaaagc accagaaggc tacgcattac ctaagaatga agctgcgtac
     5161 actgaattta cagtagatga tgatccaaca acttctgatg aaacaacgat cgataaccaa
     5221 cctaaaggaa tcttgccttc aactggtggt atggggatca tcgtattcat cttggtaggt
     5281 actgctttag ttggaggagc agtgatctat ttcaaaaaac gccatcaaga aacagaagct
     5341 taaactattg gaaacaaaaa tcatacgata aataaacgat agtgattcaa atggggagaa
     5401 aggttgtgac aagtggtcgg tctcttggta ttcgctcaac aaacggcaaa ataggaggaa
     5461 ctattttgcc gtttgcttcg ctaatactca ggtcaaggcg ctgatttcac aacctcttca
     5521 tatacatatt agaaaatagg tggacatgat gaataaaagc agaataagaa tactggacat
     5581 cttcatggca atcattttag tcgttgggat cgggatcttt tcttatcctt ttgtagaaga
     5641 ttcactgaat gattttcttg cccagcaaat gatcatccat tatcaaaagc aagcatcgaa
     5701 taagaattca gaggaaataa aaaaacagca ggaaaaaatg acaaagaaaa accaacagtt
     5761 ggcagagcaa aacgtatcac cagggatagg cagtttcaac caagcagttg atgcaaaagc
     5821 attaaaagat ttacctagta atgccttttt tatggaacat atgcttggag tcatcgaaat
     5881 cccaaaaatc aatgtcagct tacctatatt cgatcagaca acggaaatat ttttgcaaaa
     5941 aggaacgtca ttattggaag gaagttctta tccgactggc gggaaaaaca cccatgccgt
     6001 tctttctggg catcgtggac tcccagaagc aaaattattt acagatttgc ctaagttgaa
     6061 aaaaggagac cagtttttca tccagatcaa tggaaagaca ttggcgtatc aagtagaaaa
     6121 aatccaagtg gtcttacctg acgaagtaga ttctttgggt atccaaaaag gcagagatct
     6181 cgtgactttg cttacctgca ctccttatat ggtcaataca catcgactgc ttgtgacggg
     6241 gcatcgtatt ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa ttgatcaatg
     6301 gaaaaaatgg aaatttttta tttggtttat cggtatacta cttggatcaa tcggcttagt
     6361 ttggctactc atcgcttatt tagattcttt ggccatcgct aagcgaaact atcctttatc
     6421 tttttatgta aagaacacaa acggacgacc aatcgaagga atggtctttt ctgtaaaaac
     6481 gctcaacggc aaacactata ttactagaga aaaagttcca tttgtgaaag cttctgatga
     6541 atacggacta gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc aacatgagga
     6601 gctcctgctt aaaatccatg taaaacataa acattcgaaa cagttttcaa tgaaattgaa
     6661 aaaaggaaga tataaactgc gaaaagaaaa agaggtgtac tatttgattg agaaagaatg
     6721 aatgaagatg tccaaaaaag cagagggaac agactaaatg aaaaaagaga gaagctgacg
     6781 aaatggacgt gtaaactgct atttttggtt tactatctgg aacgccagct tctatttttt
     6841 tatgtttcag aagaatttta ttattatagt ataacttttg aattagtttc cataacattt
     6901 ggagcaaggg gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg tatacgtacc
     6961 atttccacat ttatgtgtat ggtattttga accggtcggt gtgacataga cagtttgttc
     7021 attattttgc tcaggagctg cggcttgtgc ctgctgagaa gcctgttcag ctgctgcttg
     7081 ctgtcgcgcc tgttctgctt gttgctgtgc gattgcttct tgcctcgcct gttctgcttg
     7141 ttgctgtgcg attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa tttcttcttg
     7201 ctttttccgt tcttcttctg cttcttttgc tttgattgtg gaatcaattg cagcaagacg
     7261 gcttgtcaac tcttgtttgt tttcaggtag cgcttggata gctgctgaag cagttgcata
     7321 gttttctttt gtaggatttg cttcagcttg tgacaaagca gattccgctt ctgtcacttt
     7381 tttttctagt gcagcttttt ccgctttttg tttctcttct tcttttttct tttcaatttg
     7441 ttctttcgat gaatctgccg tactttcgga gatcgtggta ctgctgctgg aagcctgagc
     7501 tgaacttgtg ctttgagtag tagacgtagt agcaccacac ccactcaaca aacttcctgc
     7561 gaccaaaaac aacgcaacta attttttcac taaatttcct ccttaagtaa aaaacaaccg
     7621 ttttcaataa atgtcctttt caattgttta cttacttgtt ataataattg tgttatgaat
     7681 agtgattgag gaaacttaat ctaatcccgt gttgatacac gggatttttt gctatagttc
     7741 tgttattaag tatgcaat
//
LOCUS       DARDHD010000125         7704 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000125 DARDHD010000000
VERSION     DARDHD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.125"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            427..1419
                     /locus_tag="SDA06_002089"
     CDS             427..1419
                     /locus_tag="SDA06_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4229348.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1435..2319
                     /locus_tag="SDA06_002090"
     CDS             1435..2319
                     /locus_tag="SDA06_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4229349.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2368..3975
                     /locus_tag="SDA06_002091"
     CDS             2368..3975
                     /locus_tag="SDA06_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4229350.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3976..5460
                     /locus_tag="SDA06_002092"
     CDS             3976..5460
                     /locus_tag="SDA06_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG4229351.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5637..6629
                     /locus_tag="SDA06_002093"
     CDS             5637..6629
                     /locus_tag="SDA06_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229352.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6681..7550
                     /locus_tag="SDA06_002094"
     CDS             6681..7550
                     /locus_tag="SDA06_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4229353.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2469 a         1222 c         1681 g         2332 t
ORIGIN      
        1 tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga catagcttct
       61 tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta aatcacattt
      121 tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc tgtttttatc
      181 tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa gggtgcttaa
      241 aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat tctggcacaa
      301 agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga aaatagtggt
      361 aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa tggaaacgtt
      421 tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg tggtgagata
      481 agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa gaaacagttg
      541 cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac gtttatcttt
      601 aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc tatgctagga
      661 attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag ttcaccaaat
      721 tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt gttaggtttt
      781 tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa aataaaacag
      841 aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat tgtggggatg
      901 ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa ggttacgggg
      961 caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat tttatcggga
     1021 atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc gggagtcagt
     1081 accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa gatttggcat
     1141 atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc cgccggcggt
     1201 attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa catccctgcc
     1261 tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga ttatgcctac
     1321 tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact gattgtcaac
     1381 aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa aattatgaat
     1441 agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat tgtaactttt
     1501 ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt tatggaccca
     1561 gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat acagggatat
     1621 caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt gttttactct
     1681 tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc tttgtctcga
     1741 cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat gttcgtgggt
     1801 ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga tagtgtatgg
     1861 gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg tacctatttt
     1921 agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa tgacttgcaa
     1981 atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt gtttttatat
     2041 gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa agatcccgac
     2101 ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc ttcacaagac
     2161 atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt gatcaaatac
     2221 gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt ccaaaaatat
     2281 tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac tatttgtatg
     2341 aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc aatcgttttt
     2401 acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa atcaagccct
     2461 gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt aaaaatgatg
     2521 tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt ccaacgatta
     2581 gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt tgccgaaaaa
     2641 agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc cgctgcaggt
     2701 gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga agatttgatc
     2761 gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata ccgacaaatg
     2821 tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt aggcgaagga
     2881 aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg gttggataat
     2941 ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga ggctttcaag
     3001 acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt tattaacaat
     3061 ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga taatgatgac
     3121 cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga agggtataaa
     3181 gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa agaggctttc
     3241 gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg cgtgtacttt
     3301 acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt gccagtctta
     3361 gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt ctcaagagat
     3421 cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg gatagataaa
     3481 atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga tgaagcacag
     3541 gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga ggggacagca
     3601 ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct tgattcttat
     3661 tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt gatgcatgac
     3721 acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt agaaaaagaa
     3781 gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta tcgcaaaaga
     3841 gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct tgctgaattg
     3901 gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga taattttttg
     3961 aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc aaatcgattt
     4021 attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca tttttccgca
     4081 cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca ttaccatctc
     4141 ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg gcatgcgcgc
     4201 agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga tcaaccttat
     4261 gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact atatttattt
     4321 tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt ggcggtctct
     4381 gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga aaaacagcta
     4441 ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca caacgatggt
     4501 tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt gatgtttcaa
     4561 tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac ttcagagcaa
     4621 ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt cttaatcatg
     4681 tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc taccatggct
     4741 tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt ccatgaaatg
     4801 gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg tggacgggtg
     4861 atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt aggccatctt
     4921 tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg attggtacaa
     4981 aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga aaatattgca
     5041 gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata tttacatcta
     5101 gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc taagacggat
     5161 tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg tcagaaacta
     5221 ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt tgaagtacct
     5281 ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat tgaagtcttt
     5341 attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc tcgagatatc
     5401 ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag tgtgaaataa
     5461 atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga taatgttttt
     5521 agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg aaaaaattag
     5581 taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga tcaactatga
     5641 aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact gtttcccggg
     5701 tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca gcaatgacag
     5761 agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat acgaacacgg
     5821 ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc taccatgtag
     5881 aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt ggtaatagtg
     5941 aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc atcattggaa
     6001 ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt agtattgacc
     6061 gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt ggacgtttgg
     6121 ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt gggtaccaag
     6181 aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc cgtcaactgg
     6241 ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca gaacgtgtaa
     6301 caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac gatccaatgg
     6361 cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag gatgtccaag
     6421 ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc tcaacgattg
     6481 cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct gtcattaatg
     6541 gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt gacacaacga
     6601 aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt gaaaatgagt
     6661 gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga acaaagtttg
     6721 tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt cctaccacaa
     6781 caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa ggaaatatcg
     6841 catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca agaacttacg
     6901 gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta ggagctttgg
     6961 aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct tgttatggag
     7021 aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc gttggtacag
     7081 gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt catcctgaaa
     7141 tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc tgtccttttc
     7201 atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc tttggaagaa
     7261 aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat tatcttgctc
     7321 agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta ggcgggggtg
     7381 taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa aaaatgggtt
     7441 cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt gctgacaatg
     7501 cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag aatagtattt
     7561 attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact taaatagtct
     7621 caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt caaattaata
     7681 taattacgaa atgatattga gtac
//
LOCUS       DARDHD010000126         7651 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000126 DARDHD010000000
VERSION     DARDHD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.126"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..181)
                     /locus_tag="SDA06_002095"
     CDS             complement(<1..181)
                     /locus_tag="SDA06_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG4229354.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINN"
     gene            complement(194..1297)
                     /locus_tag="SDA06_002096"
     CDS             complement(194..1297)
                     /locus_tag="SDA06_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG4229355.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1387..2343)
                     /locus_tag="SDA06_002097"
     CDS             complement(1387..2343)
                     /locus_tag="SDA06_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG4229356.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2624..4558
                     /locus_tag="SDA06_002098"
     CDS             2624..4558
                     /locus_tag="SDA06_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4229357.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5015..5431
                     /locus_tag="SDA06_002099"
     CDS             5015..5431
                     /locus_tag="SDA06_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4229358.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5445..6011
                     /locus_tag="SDA06_002100"
     CDS             5445..6011
                     /locus_tag="SDA06_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG4229359.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6034..7020
                     /locus_tag="SDA06_002101"
     CDS             6034..7020
                     /locus_tag="SDA06_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4229360.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7175..>7651)
                     /gene="scm"
                     /locus_tag="SDA06_002102"
     CDS             complement(7175..>7651)
                     /gene="scm"
                     /locus_tag="SDA06_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4229361.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2194 a         1466 c         1578 g         2413 t
ORIGIN      
        1 aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag
       61 tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag
      121 gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca
      181 tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg
      241 ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga
      301 agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac
      361 ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg
      421 tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt
      481 attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt
      541 tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga
      601 ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca
      661 agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa
      721 tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag
      781 ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga
      841 tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg
      901 caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga
      961 ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt
     1021 catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat
     1081 cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag
     1141 tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac
     1201 tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc
     1261 ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa
     1321 aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt
     1381 atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc
     1441 agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc
     1501 tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc
     1561 gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc
     1621 attatattct gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga
     1681 ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta
     1741 gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat
     1801 tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct
     1861 ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa
     1921 gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat
     1981 gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata
     2041 ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt
     2101 tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac
     2161 ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga
     2221 agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa
     2281 tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc
     2341 catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat
     2401 aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct ttcagcaaat
     2461 ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt tagacaaaag
     2521 aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta aaagaattat
     2581 catatattca ttatatagat agtctataag gagcggatga tcaatggcaa agcaaaatat
     2641 tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg caaagaaact
     2701 gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata ctatgatgac
     2761 cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat acgatctgca
     2821 gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa tcggtatcga
     2881 taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc agctgatcct
     2941 tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac acggattcac
     3001 cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga cagtagcaaa
     3061 agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg ttgtctgtgg
     3121 ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa atcgcctagc
     3181 caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg caatgccgac
     3241 gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca
     3301 caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc atatcaaatt
     3361 gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg tcaaaggaac
     3421 ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga tcgccaatga
     3481 atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg atttagtctt
     3541 ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg aacaaacagg
     3601 acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa
     3661 aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag ctaacctatt
     3721 tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg tgaatttgaa
     3781 atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc atgagttttt
     3841 ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg gcaatgtctt
     3901 atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa
     3961 aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag gatcatggtt
     4021 cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg ttgccagtgg
     4081 tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa gtgccggaga
     4141 agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag aaccaatggc
     4201 tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca tgccaaacaa
     4261 tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct tggcattagc
     4321 attgattgct ggattattca catggctatg cagtgcgaca acgattgtat tggtgatcac
     4381 gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg ttgctttcgc
     4441 attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc catggctaca
     4501 acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca agaaataaat
     4561 agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga caaaccgtca
     4621 cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc tccatgagca
     4681 agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt ccagaagtag
     4741 cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc
     4801 ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac ggtgttccac
     4861 ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg ttttctattc
     4921 ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt tatgctaaaa
     4981 ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa
     5041 aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc tttctttttt
     5101 accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact
     5161 gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag agagcaacac
     5221 atatacttac aaagatgaac acggagatta taacttgatc gagatagatg gcgatcgagt
     5281 acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa
     5341 aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc gttcaacaga
     5401 tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt aaattacaaa
     5461 aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta ttggaaaata
     5521 tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc aatctaatca
     5581 cgatcgttgc catttttacc atgaaaaaac aagacagctt cttactactt tggctacgac
     5641 tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt atgagtggtg
     5701 ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta
     5761 tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt ttgatggctt
     5821 ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca gttttaggga
     5881 ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat gtgcagacac
     5941 ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc ccaatgttta
     6001 ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat gtgggaaaac
     6061 tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga aactgtccgc
     6121 ctgaaaaaca aacctgtcga acaagccatc aaagaaacga tccatgccgg cggaaagctg
     6181 ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga tcgagacaaa
     6241 gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat ccatgatgat
     6301 atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca gtttggtaat
     6361 gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact attgtctgat
     6421 tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa agtattgaat
     6481 ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa agaatatttg
     6541 gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt tggcgctttt
     6601 gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact ttctatcggg
     6661 atggcctttc agattatcga tgatatttta gattatacaa aagagagcca ggaaatcggc
     6721 aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat ctactctttg
     6781 caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat gacagaagaa
     6841 gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga agctaggaag
     6901 ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc tgccacctct
     6961 cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg caaagactga
     7021 atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt gttttttgac
     7081 aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc aacgtccgtt
     7141 agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc ttataactca
     7201 taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt atccaattat
     7261 tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg tcattgcttg
     7321 ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag aaggagacac
     7381 ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt gaagttgatg
     7441 aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa gctgacgtgt
     7501 ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta gaggtagtat
     7561 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgttgtggta gatgtatccg
     7621 tactgtcgct tgaagctgac gtgtccgtgc t
//
LOCUS       DARDHD010000127         7598 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000127 DARDHD010000000
VERSION     DARDHD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.127"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..787)
                     /locus_tag="SDA06_002103"
     CDS             complement(59..787)
                     /locus_tag="SDA06_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229362.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="SDA06_002104"
     CDS             complement(800..2080)
                     /locus_tag="SDA06_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4229363.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="SDA06_002105"
     CDS             complement(2221..2823)
                     /locus_tag="SDA06_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4229364.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="SDA06_002106"
     CDS             3068..3274
                     /locus_tag="SDA06_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229365.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="SDA06_002107"
     CDS             3586..4305
                     /locus_tag="SDA06_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4229366.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="SDA06_002108"
     CDS             complement(4386..5069)
                     /locus_tag="SDA06_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG4229367.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="SDA06_002109"
     CDS             5287..7473
                     /locus_tag="SDA06_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG4229368.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2721 a         1282 c         1428 g         2167 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagttt
//
LOCUS       DARDHD010000128         7287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000128 DARDHD010000000
VERSION     DARDHD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.128"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            74..2683
                     /gene="clpB"
                     /locus_tag="SDA06_002110"
     CDS             74..2683
                     /gene="clpB"
                     /locus_tag="SDA06_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG4229369.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2834..3520)
                     /locus_tag="SDA06_002111"
     CDS             complement(2834..3520)
                     /locus_tag="SDA06_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229370.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3498..3992)
                     /locus_tag="SDA06_002112"
     CDS             complement(3498..3992)
                     /locus_tag="SDA06_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4229371.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4012..5232)
                     /locus_tag="SDA06_002113"
     CDS             complement(4012..5232)
                     /locus_tag="SDA06_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229372.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5379..7214
                     /gene="lepA"
                     /locus_tag="SDA06_002114"
     CDS             5379..7214
                     /gene="lepA"
                     /locus_tag="SDA06_002114"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG4229373.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2397 a         1394 c         1498 g         1998 t
ORIGIN      
        1 aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg
       61 agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga
      121 agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa
      181 aatcttttta caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt
      241 cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg
      301 aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga
      361 ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt
      421 gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa
      481 agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca
      541 agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag
      601 cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct
      661 ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc
      721 aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga
      781 taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga
      841 atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct
      901 cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga
      961 tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac
     1021 aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca
     1081 aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa
     1141 agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc
     1201 tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat
     1261 cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca
     1321 agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga
     1381 tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga
     1441 agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc
     1501 aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga
     1561 tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa
     1621 ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga
     1681 aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga
     1741 aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca
     1801 agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga
     1861 cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga
     1921 attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga
     1981 tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta
     2041 tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat
     2101 cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt
     2161 cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt
     2221 tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg
     2281 aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc
     2341 agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt
     2401 gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat
     2461 cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc
     2521 atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa
     2581 agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg
     2641 acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat
     2701 aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg
     2761 ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc
     2821 acaacctctt ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct
     2881 catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg
     2941 tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag
     3001 aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact
     3061 cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac
     3121 tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg
     3181 tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca
     3241 aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga
     3301 ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg
     3361 caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg
     3421 tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt
     3481 gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct
     3541 gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa acctattgta
     3601 tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc
     3661 gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc
     3721 gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa
     3781 gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg
     3841 tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc
     3901 tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa atcactttta
     3961 gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata
     4021 atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc
     4081 atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa
     4141 gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg
     4201 gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg
     4261 atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa
     4321 ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact
     4381 atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat
     4441 catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata
     4501 gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg
     4561 ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca
     4621 atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt
     4681 gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac
     4741 tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat
     4801 ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg
     4861 cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt
     4921 agcatattct ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg
     4981 aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca
     5041 tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt
     5101 ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc
     5161 attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc
     5221 tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc aaaattctac
     5281 aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta
     5341 taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag
     5401 aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta
     5461 cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc
     5521 agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg
     5581 tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc
     5641 cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg
     5701 ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag
     5761 cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag
     5821 atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg
     5881 tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat
     5941 atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta
     6001 tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac
     6061 ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg
     6121 tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca
     6181 cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca
     6241 atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc
     6301 tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat
     6361 tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc
     6421 gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat
     6481 tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg
     6541 gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg
     6601 tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa
     6661 tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc
     6721 gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat
     6781 tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa
     6841 gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta
     6901 tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac
     6961 taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg
     7021 cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg
     7081 tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa
     7141 tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg gacgaggacg
     7201 aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc atgtttacat
     7261 ggatttgcca ccggtttgcc accggtg
//
LOCUS       DARDHD010000129         7061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000129 DARDHD010000000
VERSION     DARDHD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.129"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..393)
                     /locus_tag="SDA06_002115"
     CDS             complement(7..393)
                     /locus_tag="SDA06_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4229374.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(390..911)
                     /locus_tag="SDA06_002116"
     CDS             complement(390..911)
                     /locus_tag="SDA06_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4229375.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(913..1947)
                     /gene="sppA"
                     /locus_tag="SDA06_002117"
     CDS             complement(913..1947)
                     /gene="sppA"
                     /locus_tag="SDA06_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4229376.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1980..3395)
                     /locus_tag="SDA06_002118"
     CDS             complement(1980..3395)
                     /locus_tag="SDA06_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG4229377.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3364..5517)
                     /locus_tag="SDA06_002119"
     CDS             complement(3364..5517)
                     /locus_tag="SDA06_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG4229378.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5775..6011
                     /locus_tag="SDA06_002120"
     CDS             5775..6011
                     /locus_tag="SDA06_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG4229379.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6030..6245
                     /locus_tag="SDA06_002121"
     CDS             6030..6245
                     /locus_tag="SDA06_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG4229380.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         2265 a         1277 c         1044 g         2475 t
ORIGIN      
        1 gcctaattaa gattttctta ttagaagcat caatcccgca ataataccta aaactccagg
       61 aattacccac aataatgtta aaagaaaaaa tggaataata gataaaatga gtaaactaat
      121 accatagacc actgtttttt gattttttct tactaaaaaa cctattatga aaatcattat
      181 cgaaaaaact agggtaattc ctccaaataa aacaacattc ccgcttagat tgactaacat
      241 atctatatcc ttactggtta gttctgtagt atttaaagta gttgaatcaa tagaagatag
      301 aaacatacca cccatcaatg ctaaaagact acataaaatc cctgttattc cactaattaa
      361 aatcaaaata aactctgttt ttctcttatt catttaattc tctccagatt aagtgtttta
      421 gaattagaaa ttttttgacc atagttaata gcttctaatg aacgaataac aaccactgac
      481 gtgttagaaa aataatcccc aatatgttgt ttcttgggcg tgaaagcagc caccaaatac
      541 ccaatttgaa atacaaaatt ttttaatata aacctacata tacactcacg aataaagata
      601 gtttgccaac ttagctcttt ttcctcaaag ctcactactc ttatcccaaa aatcattttc
      661 ccaattgttt gtcctgaatt cagcttagtc aaaagtacaa aataggttaa ataaatagct
      721 aatgacgcta taccataaaa agtaataatg gatttgtcag attccagtcc catgattttg
      781 taaaagagac ctatagtagc atgggtgatg atgctaatac aaattaaatc aataagaaaa
      841 gcaaaaaatc gtaaccaaaa tcccgcaaat acataattag gcaaagtttc aatgccactc
      901 ttcttattca ttttatagcc ctccatagta atacattggt tttggagtat cagaaaatcc
      961 aagtttttca aaaactgaaa ttacacgatc tgatgtagtt gcctttagcc cttgccattc
     1021 agcaatctta ttacctaacc aagaagaagc aaagccagtt gtgtcatttt tatattctat
     1081 aaccgtcgca tcagttaatt tcttctcttt ttttagggaa tctaatgcat cttcagagta
     1141 accaatttga tccaccaacc cattttctac tgcttggtta ccatcataaa ttcttccatc
     1201 cgcaagttct ttcactgcat tagtatccat atttcttcct tttgctacaa cattgacaaa
     1261 acgatcataa gcactcatca caaattcctg tataacttta ttctcttcct ctgaaggttt
     1321 gtgttgaggt gacatcatat cttttaatgc accactttta tacgtcgcat cagttacgcc
     1381 aagtttttct aataagccag aataatttaa accagaaata atgacaccaa ttgatccagt
     1441 tgttgtttct tccgtagcaa atattttatc tgaaccagca gaaatgtagt accctccact
     1501 tgccgctgta tttttaaatg cagtatagat aggaatatct agctttttaa tttttgccat
     1561 ttctttagca atctcagcac tttcatatat tcctccccca ggtgaattta cttctaataa
     1621 cactccctta accgctttgt cttcttgaat tttctttagc tgtgttaaaa aattctgatg
     1681 gttatattgt tccctactaa aaagattgct ttcacctgta tctgcaataa ctccattgac
     1741 ggatagtttg acaatctttt tatttgatgc gccttcttct aacgtttctt ctgtgatttc
     1801 gttagatccg taaaaaatct tatttattcc tgttagggtt gtatttgatg cttcctcttt
     1861 atcttttcct ggaattagcg cgacgataat ggatagtaac aataatccgc acgctactcc
     1921 cacagcaatc catcttcttt tattcatata aactcctcct atattcttag taaaaataat
     1981 tattttttat ttagaaacac atcgacaatt tgttgccaat atgttttttc tcctacaatt
     2041 aaagacaact ctccagttaa tccataacga aacggaaacg attgagttgt ttttaacgtt
     2101 cctttcactg cataaaaaac acctttctca gtggcagtag aattttctga aatttcatct
     2161 aaagttccgt cgatcgtaat cggagcccca cctttttgat ccaatttaaa atgaaccttc
     2221 atcccttctt taatataggt actttcattc gcaggtaaaa gtgctgtaaa tttcattttt
     2281 gatttttcgt ggatcggata aatttctgca agcactgttc ctttggctag ttctgcttgt
     2341 ccctttgttt cttcatttaa atgaaccgtt ccagatacag gagcttttag tattcctttt
     2401 gaaagttgat cgtccaaact tttgataact gactggttct tctcttgttt ttcatttagc
     2461 tctgtaactg tttgttttac agttgttatt gcttgttcta gctcttgctt tcctttttcc
     2521 gtttgactaa ttctgtcatt ctcagaagta gttggtttaa ctaatttagc tttttctgac
     2581 tgatactgtt ctacttcttt ttttagttgc tcaatttgtt catttatgct tgctgaaatt
     2641 gtcagtttca cttgattttt ttgatcatca gaaacattat ttagttgttc ttgccaattc
     2701 tcatagtttg ccatcatttc ttttgaaaaa tcttttaatg attgattatt actccaagct
     2761 acctgtactt gttgccattc ttgtaattta ctatctgaac tctcaatttg tttttctagt
     2821 tgtctttttg tcttttcgta tacttctaat tgcttttgat catttaattc actttgcttc
     2881 aaagcgtagc ggagtgattc tttttctgac aacatgctct ttaattgatt actgtagcca
     2941 aaactgtcat ccgttgaaaa caaatttttt cctttactta tactttcaat aaatagctga
     3001 gccatcttta tttgttgagt aatacgttgg ttttcttgtt ctatctgagc cttttcattc
     3061 tctattaaag aacaatccaa tctaaccaac acatcgtcct tatgaacaaa ttgattttct
     3121 tttaggaagt tttccattat tttagcttct aatggcactt gtaacttctc tattttttcg
     3181 gggttcaatt gagccgttgt ccgaataaca acctcttttt tagcaaaggt taaaaacaat
     3241 ccaataatac ataaaaaaag aattacaggg tataacaccc aacggtagaa gctatgatgt
     3301 tgtttagtat agatactaga atgttcgaac caattattat tctgcataaa ttgtttccct
     3361 ctcctagatt tcccataatt tttgatagat accatcggca aaacgaagtt catcgtgcgt
     3421 tcctttttct accaaatgcc cttcatggag aactaaaatt tgatcacatg cttttgctat
     3481 tggtaaatga tgcgcaataa aaataatcgt tttttctttt aaattgagga gataattcat
     3541 aattgcatgt tctaacaagg tatctaatcc actagttgct tcatctaaaa ttaatatgtc
     3601 cggatttttt aataaagccc gtgctaaggc taatcgttgt cgttgccctc ctgaaagatt
     3661 atttccacct tcttccaaca atgtctcgta acgtaatggc tgttgattaa taaattcatt
     3721 tagttggact atctcacaaa cttctattat ttgttcgaaa cttggtgggc tatctaaacc
     3781 gaatataaga ttctcaatga ttgttccact aaagaaaaat gactcttgag gcaaatatgt
     3841 cactcgatcg cgtaatatct gaaaaggaat gtctaagtga ttaatgtttc cataatggat
     3901 cgatccttct gatgcttcat aaaaacgaac aaataacttt gcaagagttg attttccaga
     3961 accactaact ccaactatag ccgttttact gtgcgaagga attgtacatg aaatttcgtt
     4021 caacgttggg gccttcatgc tatacgagaa tgaaacgttt tttacagtga tatcttgttg
     4081 aaataattgt tcagaaagca tatattcaaa agactggttc gatttttctg aatcaatcgc
     4141 gaaaatttca tttaaacgat tatttgcaac ttgagccgtt tgcattttca cttgtaaatt
     4201 aataatgttt tgtaaaggat ctgtcaaaaa tactagtaaa gcattgtatg tgataagttg
     4261 acctagacta attctattat ccataacaaa ataagaacct agccataaaa ttaatgcact
     4321 gctaattaat tgaatcaaat gtttaattcc ttgttgaata ttatcaagcg taaccgtacg
     4381 aaatgatttt ttcattaatt taacaaattc atggtctata cggttatata ctttctcttc
     4441 accgctatat gctttaattg tttctattcc tttaagactt tctatgatac tagaatttac
     4501 agctgcaccg gctgccattt cttccctgtt cgatttttca tagcttttca cgaaagcgaa
     4561 tattgcaact acataaaaag gcaaagagca tagagtgatt aaaaacaacg tactattctg
     4621 aatagctaac gtaaccccaa caagcaacac cataccaata tctaaaaata ttgataacgt
     4681 agcacttgct aaagcatcaa taatcttact ggcatcaaga aaacgagaaa taatttcccc
     4741 tgattttctc gtagcaaaaa aattcattgg taaagttaac acatgtttga aatactgaag
     4801 cataattgcc atactcattc gctgtcctaa aataataaga agataactac gactatattc
     4861 aaacaatacc cgaaataaat agacagcgat caagccaatg gaaattatat ttaaagttga
     4921 acgtgcttga ttagggatga aaaaatcaag aataccttga aaataatatg agcttcctat
     4981 gccaaaaaac gtaatgaaaa tcgatgctaa aacgatgtgg aatactaatc ctttttgttt
     5041 ccaaattata ggcaaaaaag aacttaatcc agctactttt tctttaatag gaatataagt
     5101 ttctctaggc ataggtaata aaagaatacc tgtccattct ttttgaaacg cttcaatcgt
     5161 ttttctattt tttccattag cgggatctgc aatccatagt acgtttccct tcgttttata
     5221 aattacaacg tagtgcatat agctgttatc aaccaatata tgtgcaatta atggtaagat
     5281 aagatctctt tctttccaga tctcactatc tgcttgaata gccatacaat caaaagacaa
     5341 tttttcaaat gtctttttca atccaaatgc tgaagtaccc tctaaatcag tcccagatag
     5401 gtcccgtaat ttatgaatcg ggatatcagt tttaaaatat tttaaaatca tcgaaaggca
     5461 agcaacacca caatcttttt catcttgttg ctgcacaaaa ggaaatgatt ttatcactta
     5521 tctaacacaa ctccttctaa aaaattttta tagaaaaatt ctcaatatag ctttaataca
     5581 gtgaaaatat tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt
     5641 tttcattcct gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag
     5701 tttcaggaag atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata
     5761 ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg
     5821 cacaaactgt tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac
     5881 ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg
     5941 gtgttgttgg agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc
     6001 gaaaaaaata aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta
     6061 tattatcttc agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca
     6121 ctactttttc tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa
     6181 tagctgcttg tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg
     6241 cgtaaacaat taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt
     6301 atgttcttta tcttttcaaa agtttggaat ggacagaact ttttactaaa gcttcttgtg
     6361 ctagttttta taattatatt aaatttttat cttttattaa agtcagataa aaattctttg
     6421 gaaatgtaat ggtagagata tttttacaaa aggacttgca gcgctgtagt tggtgctgca
     6481 ggaggaccta tttcggccgt tacaggaggt attggtaatg tacttgtcgg atgtgcatta
     6541 aagcctgttc ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc
     6601 aagtacaatc ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca
     6661 ctttctaaaa attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt
     6721 taattcccct ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt
     6781 tggttccaga ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta
     6841 ataacagcta taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa
     6901 ggaatctatt ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt
     6961 tctttcaact tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct
     7021 gaatcaaaat atagtgaacg ttttttaaaa tctatttctt t
//
LOCUS       DARDHD010000130         7019 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000130 DARDHD010000000
VERSION     DARDHD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.130"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(132..1268)
                     /locus_tag="SDA06_002122"
     CDS             complement(132..1268)
                     /locus_tag="SDA06_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4229381.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1300..2751)
                     /locus_tag="SDA06_002123"
     CDS             complement(1300..2751)
                     /locus_tag="SDA06_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4229382.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(2795..3559)
                     /locus_tag="SDA06_002124"
     CDS             complement(2795..3559)
                     /locus_tag="SDA06_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG4229383.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3587..4285)
                     /locus_tag="SDA06_002125"
     CDS             complement(3587..4285)
                     /locus_tag="SDA06_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG4229384.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4297..5076)
                     /locus_tag="SDA06_002126"
     CDS             complement(4297..5076)
                     /locus_tag="SDA06_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG4229385.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5092..6036)
                     /locus_tag="SDA06_002127"
     CDS             complement(5092..6036)
                     /locus_tag="SDA06_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4229386.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6082..6861)
                     /locus_tag="SDA06_002128"
     CDS             complement(6082..6861)
                     /locus_tag="SDA06_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229387.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1925 a         1512 c         1101 g         2481 t
ORIGIN      
        1 acttttatat ggacgatgtg tcaaaaaatc aaactgaatt ttagttcgat ctatattcct
       61 ataatagttc attagcatcg tctccaaccc tgcacgatgc atatcattca cacactgtaa
      121 tattcgaatc attatatatt tcccctatac aaatttaatg tttcttgtac gattttatcc
      181 caattatatt tactacaaat aaactcagta gcttgattct tcaaaacttt aaccatttcc
      241 gattgattgc atgcctcttc taatcttatc tttaaatcac ttacatccga tttcttaaaa
      301 atcatagcct tgtcttctac tacttccgta cactctgaga tatttgaaac tatacaacag
      361 ttcccataac tcattgcttc taacaaactt aatggcattc cttctaaatc acttggaagt
      421 gtatagatat acgcattact atataattct tctaattcct gtccttgtac aaatccagta
      481 aatataattc gctcatcacc ttttgctaat tcctttagtt catttgcaaa ttcatctgta
      541 tcagagcttc ctccggctat tataagtttt ttatctgtct gaacatcttt aaatgcctca
      601 attagatacc gaattccttt ttcgggaacc aatcgcccca aaaacaaaat ataagaatct
      661 tttgttaatc catacttttc tgtaatcaat cctgctgatt ttatttcaat ttttttaacg
      721 ccatttggta taaaccttgt ttttcttcca tatatatctt caaaataatt ttggacgctt
      781 tcactaagta caattatttc atctgcaaat tttacagcac acttttcccc taacatgatg
      841 tatgtactag caagcttgtt ccatttttct ctttgatgat ctagaccatg aattgttgca
      901 acacatcttt tcccaaaaag ttttggcaac cacaacatag cacatggtcc ttctgcatga
      961 aaatgaacaa catcatattt tccgaatgct gctctaattc caccaaatac tgaagaactc
     1021 attgctgcaa ttccttttat atttaatgta aaaatagtct ttaattttac acctttatat
     1081 tctttatttt tatgctggtc aaattccttt ccgctaacat gatgaccact tcgattatag
     1141 caagtcactt catgcccaag tgcaaccatt cgcgttgcca attcttctac cactatctca
     1201 attccacctt ctcttgatgg aattctttta tgacctagca tacagatttt taattttttt
     1261 cttgccatca cgttttactc cttctatagt atctttatct tacatggaac cgtttctatg
     1321 aagcacagtt ttgactgtcc taaacaaaat tctaaaatct aatcccatac tccaattcct
     1381 gatatattct gcatcaagtt ttacaacctc ttcgaagtct gtgatatcac ttctaccact
     1441 cacctgccac attccagtaa tcccaggttt catcgcaatt ctcgcacgat gatgaaaatc
     1501 atacttctca tattctgact tcaagcaagg tctaaacccc accaggctca tttttcccat
     1561 taggacatta taaaattgtg gaaactcatc caatgatgta gatcgaataa attgtccaat
     1621 tcctgtcttc tttgttccat caggaagtat tttattgcca atgattcttg ggtcaaaatc
     1681 catcttaaac atgagattat tactcatctt attttctttc atcaactctg ctttgcgttc
     1741 ttctgcatcc atatacatac tacggaactt atacatctta aatgtcttac cattttgacc
     1801 aatacgttct tgtgcaaaga aaatcggccc tggtgatttg atgtaaataa ctggtgctac
     1861 gaagataaaa ataacacctg ttgcaataca tccaataatt ccagcaagaa tgtcaatcgt
     1921 tctctttata aatgcttgcc tcatcgtcat aacattaata ctagatgtaa taacagtata
     1981 atcaccaact cggccaataa attgttttcc acgtgtacta tcagacactt ttgtaagatt
     2041 catatgtaca gcaagtccca tttccgaaca tctggcgatt aattctttgg gatatggaac
     2101 actttcgtca acattgatga atacttcatc tacccaattc tgtaaaagat attccgctgc
     2161 attatcagca tttgcaacta ctgggacacc tccgatggta gttccataca tatctctgtc
     2221 tactacaatc acaccactaa gtttatacat gccataattc ttctctttaa gattacgaac
     2281 tacatttttt gcaatctttt gagatgttac aataactaac gatacatctt caccgttttt
     2341 catgcggtga tgcaacaacc ctttccaaag tatacgaatt atatatgtta ataatccata
     2401 caccaccccc attataaata aagaaactct ggaataatca tttccatcct gcattgtaat
     2461 aaggtataaa ctgcacacaa gtaacagcat aaaagattgc tttatagaag ctacagcctc
     2521 tttataatat cctcttttta aaacattttt atatgcctca aataaaaata taatcgtaag
     2581 attctcaagt tctataaaaa ttgccatgtg gcgataaata ggaatcacat acggattcca
     2641 gtcaccctgt cgcattacat atgatatata aaaagcaagc tggacacaaa ataagtctaa
     2701 taaaataaaa tcaatatgtt tagaccaacc acttgagttt tttctataca tctctcactc
     2761 tctcctcatt tcctttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt
     2821 ttacctcttc atagattgga taattgatct catccccgtt gatcagatcc tttgcgactt
     2881 gtttcatctg tactactttt tccttaccaa actccttggc aatctgctca tagcactctt
     2941 ttaagtaaaa ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat
     3001 tatgtttgac cattgttttg gctgttttct ggatatcttt gccaaataca ccaacataac
     3061 ttggcgcagt aagttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt
     3121 cttctcgaaa atgcgcgttg cgttctggat ggacgatcac tggtatcttt cctagttcta
     3181 acaactcaaa aaaaagccgt tctgcatata acggcacttc cagtgtcgga aattcgatca
     3241 gaagatatgt atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga
     3301 gcgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct
     3361 ctaattcggc ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt
     3421 ttccattatt gtggtgtggt gtgcataaaa tatgcgtaat gccttgctga atcgcttttt
     3481 cagccattgc aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat
     3541 ggcaatgtag atcgatcatt tctcttgtct cctctcttct tattccttaa tttccgtagt
     3601 aataaccagc atctttgctg tgttctgctc cgttgtatac gacacctaag actcgtgctt
     3661 gtaccatatc taacagttct tttgctttag tcaatgattc ttttcgtgac gtattttccc
     3721 gtacgaccaa taaggtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcaa
     3781 cgactggcgg catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct
     3841 gatccattct tggcgaactt agtagttctg atggatttgg tggtttaggt ccacttggca
     3901 gaatcgacaa attgtcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac
     3961 taagaacagt acttaatccg cttgcgttgt tcaacttgaa cgttttatag atgacgggct
     4021 tacgtaagtc tgcatcgact agcagcaccc gttgaccaga tttggcaaaa acaacggcaa
     4081 tgtttgctgc agtggtcgat ttcccttctc caggtcctga tgaagtcaca acaatcgttt
     4141 tgatttgctg accagctgcg gaggcaaact ggatattcgt ccgaatcgta cgatattgtt
     4201 cagcgattgg tgaagaggga ttggtgagtg tgaccaaact gactgcatga gtttgggctg
     4261 ctttttgttt ctgtgttcgt gccattttct ttcctcctag actcttgagc ggcttctgcg
     4321 tgatggcgct gttttatcgt ttgtagcttt tggttgtgtc tgagctggtc cttttttacg
     4381 gataccagca ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc
     4441 taatgtatct gtcacatatt tttcatcttt cactgtccga tcgagtaatt ccaataagaa
     4501 tgccagacct acacctacca tcattcctaa cattaagcca gtcattaaat tcaatttatt
     4561 atttggcgaa acaggtgtcg tattggcaac tgcacctgaa atgatcgaga ttttatcaac
     4621 attcatcaca tcttttgcgt tctcttggaa aacttgtgaa gttgtgttag cgatctgttg
     4681 agcagccact gcattcgtac ttattgcctg aatcgagaac atttgtgaat tttctgcctg
     4741 attaactgag atggcatctt tgatttgttc agcagtcatt ttcacattgt cttctgtttc
     4801 taaacgatct ttgacttcat tgagcactaa atcactgacg atcatatctt tatacgtatt
     4861 gatcatttgc aggttcgtat tgacatcatt gacatttgca gtgtctgatt gtggcagtgt
     4921 cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc
     4981 tgagatggcc aagccagcaa acattgagat gatgattatt accaagtgtt tgcgtaatgt
     5041 ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt
     5101 ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt gatcactgac
     5161 tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttaat
     5221 cgtaccaaat gcctcccgat aatctaaagc aatttttttc atttcatcaa agttcatgtc
     5281 tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga caccgtttag
     5341 actcaatact tttttcgcta cgccttctac gatctttctt tggcgccctt gtctaccgta
     5401 atcaccattt gggtcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa
     5461 tgtaatttta ccgattggaa agtcatagcc atcttgtgag aaagtgaggt cattattgac
     5521 ttcgatacca ccaactgcat caacgagttc ctttagccct ttcatattga tagagacata
     5581 gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc
     5641 gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt
     5701 atcacgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat
     5761 catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa
     5821 tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt
     5881 tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc
     5941 tcctactcct acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat
     6001 ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg
     6061 cttgaatcaa cgttttaact gtcaatagct tatattgcta tagcccattt gctcagcaac
     6121 agtttgtgct tttgttgcgt atgctgcgct tattttttca ttccggatct gctctatttg
     6181 actgagcacc gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa
     6241 catcgtatcg aataactgat ctagttgcgt gacttgcgcg atctgaatat cagcgaatcc
     6301 tagatattgc ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg
     6361 tacacgagcg acatctgcac ttgtcacctt ttctttgatg atgacatcgt ctacagcttg
     6421 ctgccagttt gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt
     6481 tgattctgca ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc
     6541 ttctttaggc aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga
     6601 tagcttattt gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt
     6661 ttcttttgta ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg
     6721 attggtgatg gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat
     6781 atggtatcca aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga
     6841 cttgacgaat ggcttttcca tagattcact cctaagtatt gatgatagat aaattaagat
     6901 ataattcact tacataaaaa tgacagcttt ttatttaaaa aaacataatt aaactaatat
     6961 attcacagaa atatttgtaa acaagttttc cctatataat cataccacaa actgttttc
//
LOCUS       DARDHD010000131         6935 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000131 DARDHD010000000
VERSION     DARDHD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.131"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1026
                     /locus_tag="SDA06_002129"
     CDS             169..1026
                     /locus_tag="SDA06_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4229388.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1019..1921
                     /locus_tag="SDA06_002130"
     CDS             1019..1921
                     /locus_tag="SDA06_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4229389.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1954..2304
                     /locus_tag="SDA06_002131"
     CDS             1954..2304
                     /locus_tag="SDA06_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4229390.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2353..3276
                     /locus_tag="SDA06_002132"
     CDS             2353..3276
                     /locus_tag="SDA06_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4229391.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3354..4247
                     /locus_tag="SDA06_002133"
     CDS             3354..4247
                     /locus_tag="SDA06_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4229392.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4285..4848
                     /gene="amaP"
                     /locus_tag="SDA06_002134"
     CDS             4285..4848
                     /gene="amaP"
                     /locus_tag="SDA06_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4229393.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4862..5050
                     /locus_tag="SDA06_002135"
     CDS             4862..5050
                     /locus_tag="SDA06_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4229394.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5063..5965
                     /locus_tag="SDA06_002136"
     CDS             5063..5965
                     /locus_tag="SDA06_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4229395.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5997..6239
                     /locus_tag="SDA06_002137"
     CDS             5997..6239
                     /locus_tag="SDA06_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4229396.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6495..6866)
                     /locus_tag="SDA06_002138"
     CDS             complement(6495..6866)
                     /locus_tag="SDA06_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229397.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2527 a         1182 c         1385 g         1841 t
ORIGIN      
        1 ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg ataaaaaaat
       61 tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat gaggaatatt
      121 ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat ggagaagaga
      181 tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta cttgagcgtg
      241 atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt ttatgtgatt
      301 gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa taaatattca
      361 agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga cttcctagtc
      421 agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt attagctaaa
      481 gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga cgatatcgta
      541 aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga tccaggccaa
      601 gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa ttcaggagtg
      661 atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac tatccattac
      721 ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa tgctgtactt
      781 tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct gatttttgaa
      841 cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct
      901 tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga tcatccgtat
      961 accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg cgagatcaat
     1021 gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac cacatttagc
     1081 tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc ggttctatgt
     1141 gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc
     1201 acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta caaatgtggt
     1261 aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg aattgttccg
     1321 cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt tacaagatat
     1381 ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg aagacgctgg
     1441 gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc
     1501 aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt
     1561 agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg cattaaatgc
     1621 tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag gctatatcat
     1681 ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag aaactcgcaa
     1741 caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg attttgaagg
     1801 gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt gtgtagctaa
     1861 attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata
     1921 atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc taaaaatgca
     1981 gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat
     2041 gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc
     2101 attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc attattggac
     2161 atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat ggacttcaac
     2221 aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta taatatcaaa
     2281 ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag gtatacattg
     2341 agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa aaaatataat
     2401 tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga agaattcgag
     2461 cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg gatggagcgc
     2521 ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct agatcagtca
     2581 gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga taatctcttg
     2641 attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa gcatggaaat
     2701 catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta cacccattac
     2761 gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc agctcgtaaa
     2821 acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat cgtatatatc
     2881 gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc tgaaactttc
     2941 atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca gcgacaaaca
     3001 gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct attcaacggg
     3061 atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc attaatcatt
     3121 tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt accgggtaaa
     3181 agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc agtagtttta
     3241 ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa acaggtatca
     3301 aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca gcaatgacag
     3361 aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct aaacaagatc
     3421 aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga gaagaatcct
     3481 atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga gattcaggga
     3541 tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct cttcaattct
     3601 tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat gcaggacgta
     3661 agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa atcatagata
     3721 aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga cagcaaattt
     3781 cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt atgataaaca
     3841 tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca ggaggagcaa
     3901 tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta gattacgcag
     3961 caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt gcacctaaag
     4021 gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa ctagacgaag
     4081 gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc
     4141 agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca agttatgtta
     4201 cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa gttgatcgtt
     4261 tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga
     4321 acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac ctatccattg
     4381 ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt gcaacaatat
     4441 gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact tgttttcctg
     4501 ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga gttgacgttg
     4561 gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga attcatccat
     4621 tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag
     4681 ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga aatcgaaaat
     4741 gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa atacacaggc
     4801 tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg ggggaccaaa
     4861 aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac ttggattgat
     4921 attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct tgatcatggc
     4981 agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag atcactattt
     5041 taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca ataaaatgaa
     5101 caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat caaatggtgt
     5161 gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag tagtccctaa
     5221 cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc
     5281 aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt ctaaacctgt
     5341 ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta gttctgacca
     5401 cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa agcatgattc
     5461 tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg tgcaaaaaat
     5521 catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg gtttcttctc
     5581 taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg aaacagaagt
     5641 cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca aagatatcga
     5701 aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata tgacacattt
     5761 agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa
     5821 agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc
     5881 ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga
     5941 aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg
     6001 gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga gcaatcacga
     6061 ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt ggatcagcac
     6121 ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca atcgtgccat
     6181 cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa
     6241 aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa gaacagccaa
     6301 acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga tgcaagcttt
     6361 tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag cctcttattt
     6421 atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt ctttattgat
     6481 acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg
     6541 aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg gatcgtatga
     6601 tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa gacaaggaag
     6661 aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt aaagtaatct
     6721 agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc atgttgctct
     6781 gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa
     6841 agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat
     6901 tttaaaataa cgatatttgt ttgtgaagct ttttc
//
LOCUS       DARDHD010000132         6903 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000132 DARDHD010000000
VERSION     DARDHD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.132"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            190..1062
                     /locus_tag="SDA06_002139"
     CDS             190..1062
                     /locus_tag="SDA06_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229398.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1059..3017
                     /locus_tag="SDA06_002140"
     CDS             1059..3017
                     /locus_tag="SDA06_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4229399.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3349..4200)
                     /locus_tag="SDA06_002141"
     CDS             complement(3349..4200)
                     /locus_tag="SDA06_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4229400.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4323..4790
                     /locus_tag="SDA06_002142"
     CDS             4323..4790
                     /locus_tag="SDA06_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG4229401.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5053..5535)
                     /locus_tag="SDA06_002143"
     CDS             complement(5053..5535)
                     /locus_tag="SDA06_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG4229402.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5635..6498)
                     /locus_tag="SDA06_002144"
     CDS             complement(5635..6498)
                     /locus_tag="SDA06_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4229403.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2067 a         1421 c         1253 g         2162 t
ORIGIN      
        1 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
       61 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
      121 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
      181 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
      241 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
      301 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
      361 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
      421 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
      481 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
      541 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
      601 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
      661 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
      721 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
      781 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
      841 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
      901 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
      961 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
     1021 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
     1081 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
     1141 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
     1201 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
     1261 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
     1321 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
     1381 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
     1441 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
     1501 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
     1561 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
     1621 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
     1681 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
     1741 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
     1801 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
     1861 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
     1921 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
     1981 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
     2041 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
     2101 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
     2161 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
     2221 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
     2281 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
     2341 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
     2401 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
     2461 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
     2521 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
     2581 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
     2641 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
     2701 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
     2761 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
     2821 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
     2881 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
     2941 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
     3001 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
     3061 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
     3121 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
     3181 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
     3241 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
     3301 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
     3361 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
     3421 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
     3481 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
     3541 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
     3601 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
     3661 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
     3721 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
     3781 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
     3841 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
     3901 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
     3961 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
     4021 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
     4081 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
     4141 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
     4201 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
     4261 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
     4321 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
     4381 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
     4441 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
     4501 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
     4561 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
     4621 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
     4681 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
     4741 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
     4801 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
     4861 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
     4921 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
     4981 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
     5041 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
     5101 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
     5161 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
     5221 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
     5281 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
     5341 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
     5401 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
     5461 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
     5521 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
     5581 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
     5641 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
     5701 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
     5761 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
     5821 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
     5881 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
     5941 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
     6001 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
     6061 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
     6121 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
     6181 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
     6241 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
     6301 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
     6361 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
     6421 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
     6481 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
     6541 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
     6601 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
     6661 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
     6721 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
     6781 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
     6841 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
     6901 aaa
//
LOCUS       DARDHD010000133         6827 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000133 DARDHD010000000
VERSION     DARDHD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.133"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDA06_002145"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDA06_002145"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG4229404.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDA06_002146"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDA06_002146"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG4229405.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SDA06_002147"
     CDS             complement(2303..2965)
                     /locus_tag="SDA06_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4229406.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SDA06_002148"
     CDS             3149..4795
                     /locus_tag="SDA06_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4229407.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SDA06_002149"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SDA06_002149"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4229408.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..>6827)
                     /locus_tag="SDA06_002150"
     CDS             complement(5976..>6827)
                     /locus_tag="SDA06_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEG4229409.1"
                     /translation="KDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILVNILARL
                     SITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFAR
                     NKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKE
                     NVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFM
                     ENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSK
                     KENQVTS"
BASE COUNT         2117 a         1268 c         1282 g         2160 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtcttt
//
LOCUS       DARDHD010000134         6786 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000134 DARDHD010000000
VERSION     DARDHD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.134"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            145..792
                     /locus_tag="SDA06_002151"
     CDS             145..792
                     /locus_tag="SDA06_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4229410.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    921..1137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1185..3830
                     /locus_tag="SDA06_002152"
     CDS             1185..3830
                     /locus_tag="SDA06_002152"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG4229411.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4140..5453
                     /locus_tag="SDA06_002153"
     CDS             4140..5453
                     /locus_tag="SDA06_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG4229412.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5443..6096
                     /locus_tag="SDA06_002154"
     CDS             5443..6096
                     /locus_tag="SDA06_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4229413.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6151..6702
                     /gene="radC"
                     /locus_tag="SDA06_002155"
                     /pseudo
     CDS             6151..6702
                     /gene="radC"
                     /locus_tag="SDA06_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         2237 a         1149 c         1480 g         1920 t
ORIGIN      
        1 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc
       61 cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg
      121 aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact
      181 gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc aggtataagt
      241 agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga
      301 gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt
      361 atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga
      421 tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat
      481 atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg
      541 atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc
      601 tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta
      661 accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa
      721 tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa
      781 gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct
      841 ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa
      901 aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt
      961 cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg
     1021 agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg
     1081 caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac
     1141 gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata
     1201 acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag
     1261 aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc
     1321 cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag
     1381 atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg
     1441 accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt
     1501 ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt
     1561 atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg
     1621 aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat
     1681 acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa
     1741 cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga
     1801 gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga
     1861 tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg
     1921 gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg
     1981 tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact
     2041 ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga
     2101 tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg
     2161 tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg
     2221 gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa
     2281 aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat
     2341 tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg
     2401 taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag
     2461 gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg
     2521 atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag
     2581 atacggaaag cgaagattat aagcgatact tccctactag cactttagta accggttatg
     2641 acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac
     2701 gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga
     2761 gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg
     2821 ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg
     2881 aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta
     2941 tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg
     3001 ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg
     3061 atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt
     3121 gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac
     3181 aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa
     3241 tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag
     3301 tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga
     3361 tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac
     3421 cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt
     3481 tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa
     3541 gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct
     3601 tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg
     3661 aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt
     3721 ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga
     3781 agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa
     3841 agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga
     3901 aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg
     3961 catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca
     4021 agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata
     4081 cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga
     4141 tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg
     4201 gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa
     4261 cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat
     4321 tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg
     4381 aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa
     4441 aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat
     4501 tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga
     4561 ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga
     4621 cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa
     4681 tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg
     4741 ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg
     4801 tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat
     4861 ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac
     4921 atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa
     4981 agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa
     5041 gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg
     5101 caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt
     5161 tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac
     5221 caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag
     5281 tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag
     5341 atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca
     5401 tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg
     5461 atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa
     5521 gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt
     5581 gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca
     5641 agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga
     5701 gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt
     5761 cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac
     5821 aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca
     5881 gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg
     5941 attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc
     6001 atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt
     6061 tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta
     6121 ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct
     6181 actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag
     6241 gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat gtcgattgct
     6301 ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag
     6361 ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa
     6421 ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac
     6481 gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt
     6541 atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg
     6601 aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca
     6661 aggatcgttt tagcgcataa tcatccttct ggtaatgttt taaccatagt ataataaata
     6721 atagtagtta aagtagttta agaggagata ttaaagggat attttacttt aaataaaaat
     6781 taacaa
//
LOCUS       DARDHD010000135         6720 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000135 DARDHD010000000
VERSION     DARDHD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.135"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..1062
                     /locus_tag="SDA06_002156"
     CDS             52..1062
                     /locus_tag="SDA06_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4229414.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1131..2075)
                     /locus_tag="SDA06_002157"
     CDS             complement(1131..2075)
                     /locus_tag="SDA06_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4229415.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2059..4779)
                     /locus_tag="SDA06_002158"
     CDS             complement(2059..4779)
                     /locus_tag="SDA06_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229416.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4776..5996)
                     /locus_tag="SDA06_002159"
     CDS             complement(4776..5996)
                     /locus_tag="SDA06_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG4229417.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6277..>6720)
                     /locus_tag="SDA06_002160"
     CDS             complement(6277..>6720)
                     /locus_tag="SDA06_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEG4229418.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1982 a         1281 c         1091 g         2366 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg
     1081 aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct
     1141 tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt
     1201 tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga
     1261 tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca
     1321 tggtgtgcta aaaccatatg acgtaataca agaacatctt cgtcagcttc gctgattttc
     1381 aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt
     1441 cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca
     1501 tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc atcggcgatt
     1561 gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga
     1621 tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag
     1681 cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct
     1741 tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca
     1801 tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct
     1861 cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa
     1921 gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat
     1981 ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac
     2041 tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac
     2101 gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat
     2161 ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc
     2221 ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga
     2281 gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata
     2341 gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt
     2401 ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg
     2461 taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag
     2521 tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata
     2581 aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg
     2641 attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg
     2701 cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc
     2761 cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac
     2821 aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat
     2881 ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg
     2941 atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat
     3001 tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg
     3061 gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac
     3121 gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt
     3181 atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg
     3241 tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag
     3301 atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt
     3361 tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa
     3421 aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc
     3481 catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa
     3541 tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt
     3601 agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc
     3661 cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca
     3721 atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa
     3781 tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag
     3841 ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg
     3901 cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga
     3961 gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt
     4021 gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa
     4081 ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg
     4141 ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc
     4201 ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt
     4261 gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc
     4321 cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc
     4381 catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc
     4441 gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt
     4501 tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag
     4561 ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc
     4621 ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc
     4681 ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca
     4741 ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa
     4801 aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt
     4861 tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa
     4921 tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt
     4981 ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt
     5041 atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc
     5101 aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca
     5161 tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct
     5221 ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt
     5281 tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta
     5341 gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc
     5401 acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg
     5461 aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg
     5521 taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga
     5581 cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc
     5641 tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct
     5701 ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac
     5761 cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa
     5821 ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt
     5881 gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc
     5941 agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa
     6001 aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg
     6061 aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt
     6121 tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt
     6181 cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt
     6241 atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa
     6301 tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat
     6361 ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc
     6421 atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa
     6481 acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc
     6541 cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa
     6601 ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctataat
     6661 ttcctcaata ttattataag ggattgaaca aatagatttt tcatactcaa gctcatagtt
//
LOCUS       DARDHD010000136         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000136 DARDHD010000000
VERSION     DARDHD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.136"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SDA06_002161"
     CDS             725..811
                     /locus_tag="SDA06_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4229419.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SDA06_002162"
     CDS             complement(932..1621)
                     /locus_tag="SDA06_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229420.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SDA06_002163"
     CDS             complement(1628..1906)
                     /locus_tag="SDA06_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229421.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SDA06_002164"
     CDS             complement(1866..2780)
                     /locus_tag="SDA06_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG4229422.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SDA06_002165"
     CDS             complement(2762..3142)
                     /locus_tag="SDA06_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG4229423.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SDA06_002166"
     CDS             complement(3592..3879)
                     /locus_tag="SDA06_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4229424.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SDA06_002167"
     CDS             complement(3899..4123)
                     /locus_tag="SDA06_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG4229425.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="SDA06_002168"
     CDS             complement(4578..4748)
                     /locus_tag="SDA06_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229426.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SDA06_002169"
     CDS             complement(4763..5296)
                     /locus_tag="SDA06_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4229427.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SDA06_002170"
     CDS             complement(5317..6159)
                     /locus_tag="SDA06_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229428.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARDHD010000137         6159 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000137 DARDHD010000000
VERSION     DARDHD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.137"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..2542
                     /locus_tag="SDA06_002171"
     CDS             101..2542
                     /locus_tag="SDA06_002171"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG4229429.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="SDA06_002172"
     CDS             complement(2872..3576)
                     /locus_tag="SDA06_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229430.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="SDA06_002173"
     CDS             complement(3763..4008)
                     /locus_tag="SDA06_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229431.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="SDA06_002174"
     CDS             complement(4025..4876)
                     /locus_tag="SDA06_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4229432.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="SDA06_002175"
     CDS             5042..6046
                     /locus_tag="SDA06_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG4229433.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1979 a         1065 c         1303 g         1812 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaa
//
LOCUS       DARDHD010000138         6108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000138 DARDHD010000000
VERSION     DARDHD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.138"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1352)
                     /locus_tag="SDA06_002176"
     CDS             complement(<1..1352)
                     /locus_tag="SDA06_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229434.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     "
     gene            complement(1650..2585)
                     /gene="murB"
                     /locus_tag="SDA06_002177"
     CDS             complement(1650..2585)
                     /gene="murB"
                     /locus_tag="SDA06_002177"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG4229435.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2658..3578)
                     /locus_tag="SDA06_002178"
     CDS             complement(2658..3578)
                     /locus_tag="SDA06_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4229436.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3691..4443
                     /gene="xth"
                     /locus_tag="SDA06_002179"
     CDS             3691..4443
                     /gene="xth"
                     /locus_tag="SDA06_002179"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG4229437.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5307..5840
                     /locus_tag="SDA06_002180"
     CDS             5307..5840
                     /locus_tag="SDA06_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG4229438.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1840 a         1173 c         1023 g         2072 t
ORIGIN      
        1 caatcagata aataaatatt cgtacgagaa gtgattcgac tctcaatcac aatattgtaa
       61 tttttaaatc ctttcacttg agaaataatg taatcggtat aagcagatcc ttgcgaaaaa
      121 tcaatacaga catgaatcgg tgcttcaaaa tatgataccg gacaaatacc gaccaataaa
      181 aagagataat cataaaataa gcgaatcatg aaacgttcat cttttcccaa gaaatagcgt
      241 ttattctctt gtatgacctt ttggattgct gtactgaata ttggatacgt ctcgaagaaa
      301 aactgtagct gctttttaga cgtaaagcta gtcatctcaa agtcgaatat ccaatttttt
      361 cgattgacat gcatcatacc catacgaagt tcttttcgta tatatagtct ttcttcttca
      421 gagatttcaa tcgaaaatgg taattgatca atcaattcca ctgacatttt ttgactaatc
      481 atgtggatat catgaaaatg agcttccgca agtccaaatg cgccatctaa ttcaaattct
      541 tcttccgtga tcaaaaacaa catgatataa gaaatctcat caatgatctg ctctttaggg
      601 atatgaagaa aagaaatatg tgataaatcc ttgatgatct tcattccttc tgagctctta
      661 aagtattcat ctgaaaagaa acgttttccg attggttgat tcccataggc cctcaacaaa
      721 ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg cagacagttt taaattgaac
      781 agatattcaa aatattgcac gatactcttt gcttcattca tgacgctttc tctaaatggc
      841 atagaaaaac cattaaagac cccgaaatat accccaaata atacgttccg tatgtttatt
      901 tcttccccaa ctaatatgtt tttctttaac tttatttgat gctgttcaag aaaagagact
      961 aattctcttc tttttacata ggctcttgat atgcttaaaa aatgattctc tgcaaatttt
     1021 tcgatcgtta cttttgaatc cacgatctcc gttaacagtc gagcaatttt tgatcgttga
     1081 aagtagattc ttcttaaatt tttgatatcc gtcaaattca gacgttcgac atggatctct
     1141 gcttcttctg aaatttggat ctctggcttt gttttgaatt cagtaaattc tttatttaat
     1201 tctatgatat aatttcttgt tttaaattta gacaaaccta atgtttcata taacttaggc
     1261 agcactacat aattcttttt ctcaaattct aaatatctag ctagcagcag tttttctcta
     1321 tcttcctctt ctataaggta atccaacttc atatcctaca cctcaaaaaa tgcctcatac
     1381 caattacttg atatgcagat gaaagttcat cttttcacct atactgattt tatgatggat
     1441 tatgcttgtt ttttcatcag ttattctcat tttttatctt ttttttgaga atattgcaaa
     1501 aaatggaaaa atttatgagg aaatattcta tattttttgt tttattgtgt taaaatgtac
     1561 aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc aggacggcca ccgtcctgct
     1621 cttttactta ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat
     1681 attgcttgtt tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct
     1741 ttgattactt cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg
     1801 atattgacga tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct
     1861 tgcaatcctg cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca
     1921 cttccgcatg aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc
     1981 attcttgctt tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg
     2041 atagcacgta attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc
     2101 aaagttttca atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc
     2161 tctccgccat acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg
     2221 caagcaaatt caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt
     2281 gaggcacctg cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc
     2341 agcatgatga ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct
     2401 aataccatcc aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct
     2461 tgttttttcg ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc
     2521 atcaatggtt catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta
     2581 ttcactgagg attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac
     2641 aatcttgcca gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat
     2701 aattcatgac ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa
     2761 cggatctgag aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt
     2821 gtccagtcgc caaattcttt cacttttttt ccatcagcct cttgaatgac aagctgggtc
     2881 agatcgttta cttcataagt accattttta cttgttactt gatatttttc tatattagcg
     2941 ccacttgcta aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg
     3001 gcatattcca aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca
     3061 tctaataaat acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct
     3121 gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata
     3181 cgtttcaatt cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt
     3241 tggttcttat cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt
     3301 ttgtcaatat aaacatggat accattttct agacattttt tggctaattc ataatgtact
     3361 tttgttgctg cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt
     3421 tcatatagat gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt
     3481 gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt
     3541 tctgcgatat tccctacacc gattacacca atcttcatct actttcctcc tggatcattt
     3601 agttactttc atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta
     3661 aggaaattaa ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg
     3721 cttcgtgcca tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt
     3781 ttctgtctgc aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat
     3841 tatcaatatt ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa
     3901 aaaccagcat tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt
     3961 ctgattactt tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa
     4021 agcgaattaa aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg
     4081 gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa
     4141 aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa
     4201 agagcggctc tttctcgact gttggataac gggtttatcg atacattccg ttatttttat
     4261 ccaacccagg aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat
     4321 gctggttggc ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat
     4381 gcaaagatcc atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa
     4441 taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg
     4501 ctttccacat tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt
     4561 atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     4621 gttcctcata tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg
     4681 tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa
     4741 atagttcctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
     4801 ccgtttatta agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca
     4861 aaaatagttc ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga
     4921 acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat
     4981 ggcaaaatag ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg
     5041 aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt
     5101 tttgaaaatg gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc
     5161 cccatctaac tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt
     5221 acataaaaaa ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag
     5281 taatttccca aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca
     5341 atcatcagcc ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg
     5401 ttgatgaatt ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa
     5461 ttcacggaat ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa
     5521 ctatccaacc atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata
     5581 caaaggtctt agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt
     5641 tatgtacagt gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca
     5701 ctgtgtgcga aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc
     5761 gtgcatgtgc ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa
     5821 aacttgtgac tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc
     5881 cgtttcactc cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata
     5941 ttgtgactgg tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa
     6001 agctgttctg catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
     6061 ttggttggac ttattaccgc agatgcaagc ttttggacac cgtttacc
//
LOCUS       DARDHD010000139         6078 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000139 DARDHD010000000
VERSION     DARDHD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.139"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            10..507
                     /locus_tag="SDA06_002181"
     CDS             10..507
                     /locus_tag="SDA06_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229439.1"
                     /translation="MSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTL
                     IAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLC
                     LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYF
                     YRNKL"
     gene            complement(633..1310)
                     /gene="nth"
                     /locus_tag="SDA06_002182"
     CDS             complement(633..1310)
                     /gene="nth"
                     /locus_tag="SDA06_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG4229440.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1317..2015)
                     /locus_tag="SDA06_002183"
     CDS             complement(1317..2015)
                     /locus_tag="SDA06_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4229441.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2283..3467
                     /locus_tag="SDA06_002184"
     CDS             2283..3467
                     /locus_tag="SDA06_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG4229442.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3473..3916)
                     /locus_tag="SDA06_002185"
     CDS             complement(3473..3916)
                     /locus_tag="SDA06_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4229443.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4142..4429)
                     /locus_tag="SDA06_002186"
     CDS             complement(4142..4429)
                     /locus_tag="SDA06_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229444.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4600..5952)
                     /locus_tag="SDA06_002187"
     CDS             complement(4600..5952)
                     /locus_tag="SDA06_002187"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG4229445.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1614 a         1197 c         1010 g         2257 t
ORIGIN      
        1 ggcttcattt tgagttatct gatagtatgc ttaattatca taatttgtct gcaccatctt
       61 tttttatcga aaacaatacc tcgttattcc aagaataaaa aagctgaaaa attttgttta
      121 ttattaaata aatttacttt aattgctcct atccttgcat ttattatttt ttctgtactt
      181 ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat gattctcact
      241 tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa accgcccaaa
      301 tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat catttttatt
      361 acacctttag atagatacgt cacttattta tataatcctt tggaatactg gacttatttt
      421 ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac
      481 ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat
      541 ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt
      601 catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc
      661 ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc
      721 tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt
      781 gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc
      841 gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact
      901 gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca
      961 ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc
     1021 agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt
     1081 attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag
     1141 gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc
     1201 actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac
     1261 attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac
     1321 tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct
     1381 acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt
     1441 gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa
     1501 gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct
     1561 tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct
     1621 tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt
     1681 ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga
     1741 tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga
     1801 cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc
     1861 aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat
     1921 tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt
     1981 tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt
     2041 atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg
     2101 gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt
     2161 cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa
     2221 acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt
     2281 acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca
     2341 ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc
     2401 actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg
     2461 ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc
     2521 ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga
     2581 tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct
     2641 tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga
     2701 tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg
     2761 agttggggaa ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt
     2821 atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt
     2881 tctcccatgt aagacctttt caccaattac aacaattatt caagatttca tccgttattc
     2941 ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt
     3001 ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt
     3061 tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat
     3121 tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca
     3181 tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg
     3241 gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag
     3301 ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca
     3361 tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg
     3421 gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc
     3481 tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt
     3541 ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt
     3601 gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct
     3661 cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga
     3721 tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt
     3781 tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca
     3841 tttctggttt attacgatga ataatctcat tagctaaatc cagatattct tctattagca
     3901 gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc
     3961 tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg
     4021 ggttgacaaa gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat
     4081 cggtaattgt gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc
     4141 tttaaagatc atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat
     4201 tcaatgtgcc gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa
     4261 cattatcttt ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact
     4321 gagagagtac ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt
     4381 tataatcttc taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca
     4441 cctttctttt caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc
     4501 tgcttattgt gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga
     4561 cagagggtcg tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc
     4621 gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg
     4681 gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga
     4741 catggtattt gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg
     4801 attcgttcct ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc
     4861 tggacgataa ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct
     4921 ttctttggtc acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc
     4981 ttgagtacca cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gcaaaccttg
     5041 acgaacggca tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac
     5101 tactgcagag tctccagctg caaaaatgtc tggattactt gtctgcatgt attcattcac
     5161 tttgattgcc ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg
     5221 gaagcccaca cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg
     5281 tttcactttt ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt
     5341 aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt
     5401 attcaagatg cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc
     5461 ttctactaat tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt
     5521 cgcttgtgca ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc
     5581 atcgattcca ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc
     5641 ataagaaacc gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc
     5701 tgtatcgacg tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc
     5761 ttcaggattt gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc
     5821 gataccgcaa gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc
     5881 tggatgattt tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac
     5941 tactactttc atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa
     6001 cagaaataaa acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt
     6061 ttcattcctt ttcaagca
//
LOCUS       DARDHD010000140         6002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000140 DARDHD010000000
VERSION     DARDHD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.140"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDA06_002188"
     CDS             <1..395
                     /locus_tag="SDA06_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229446.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SDA06_002189"
     CDS             398..1228
                     /locus_tag="SDA06_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG4229447.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SDA06_002190"
     CDS             1244..2095
                     /locus_tag="SDA06_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4229448.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SDA06_002191"
     CDS             2092..2451
                     /locus_tag="SDA06_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG4229449.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SDA06_002192"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SDA06_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SDA06_002193"
     CDS             2624..2929
                     /locus_tag="SDA06_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229450.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SDA06_002194"
     CDS             2929..3240
                     /locus_tag="SDA06_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG4229451.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SDA06_002195"
     CDS             3237..3410
                     /locus_tag="SDA06_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229452.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SDA06_002196"
     CDS             3407..3823
                     /locus_tag="SDA06_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4229453.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SDA06_002197"
     CDS             3823..4491
                     /locus_tag="SDA06_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4229454.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SDA06_002198"
     CDS             4488..4865
                     /locus_tag="SDA06_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229455.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SDA06_002199"
     CDS             4862..5161
                     /locus_tag="SDA06_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229456.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SDA06_002200"
     CDS             5151..5309
                     /locus_tag="SDA06_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229457.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SDA06_002201"
     CDS             5403..5819
                     /locus_tag="SDA06_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4229458.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARDHD010000141         5941 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000141 DARDHD010000000
VERSION     DARDHD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.141"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            314..1003
                     /locus_tag="SDA06_002202"
     CDS             314..1003
                     /locus_tag="SDA06_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229459.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            990..2198
                     /locus_tag="SDA06_002203"
     CDS             990..2198
                     /locus_tag="SDA06_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4229460.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2492..3763
                     /gene="serS"
                     /locus_tag="SDA06_002204"
     CDS             2492..3763
                     /gene="serS"
                     /locus_tag="SDA06_002204"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG4229461.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4312..5796)
                     /gene="guaB"
                     /locus_tag="SDA06_002205"
     CDS             complement(4312..5796)
                     /gene="guaB"
                     /locus_tag="SDA06_002205"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG4229462.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1819 a         1123 c         1159 g         1840 t
ORIGIN      
        1 aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt gaaagtcaat
       61 aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag ttctggtcct
      121 ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca gtttaatgtt
      181 catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt tccttaacaa
      241 agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca atatgactct
      301 ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg ttgaattatt
      361 aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg ggaaagaagc
      421 attatctaaa atcagaacaa tccctgatat tgaattactt attttagata tcatgatgcc
      481 tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat
      541 tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg ctggtgcaga
      601 tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa agtctttatt
      661 acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag taaatgcatt
      721 aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa tccagttaac
      781 agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag tatttagcgc
      841 ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta aaactgtcat
      901 ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg aaaaagtcat
      961 tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga gcaactgaaa
     1021 aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg gaaggaatca
     1081 tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc agttcggtga
     1141 tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt tttgcagaac
     1201 gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt ttctttctat
     1261 tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg caattgcggc
     1321 atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt atcccttttg
     1381 aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg gatagtaccg
     1441 tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg
     1501 tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga ctgatagaag
     1561 atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct tatatcaaag
     1621 caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta cgccagcctg
     1681 ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag ttagcagctg
     1741 attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca aaagtagact
     1801 cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat cttttgacga
     1861 atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt
     1921 cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag gcaatcgata
     1981 atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact ggaggaactg
     2041 gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac atttatgcaa
     2101 aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat
     2161 tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat tcttattaca
     2221 attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt cagagagctg
     2281 atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta cgtgcccttt
     2341 cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag ctgacgctga
     2401 acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg atggatcagt
     2461 ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa
     2521 tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa cattagctga
     2581 attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa
     2641 atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc
     2701 tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg atactgaaat
     2761 tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc ttccccatga
     2821 ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt ggagcgaacc
     2881 acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt
     2941 agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca aaggattagg
     3001 tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt acgaacacgg
     3061 ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg gtacgggaca
     3121 atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa cattaatccc
     3181 aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg gtgcagattt
     3241 acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa gtgctggtcg
     3301 ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg tcaaattttc
     3361 ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag aagatatctt
     3421 gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca tgggcttttc
     3481 tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt atcgtgaaat
     3541 cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc gttatcgcga
     3601 taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg cagttggccg
     3661 tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca cagttccaga
     3721 agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct gtgaaaaagc
     3781 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
     3841 attggactta ataccgagaa actggctttt gaacaccatt tatcgagagg ttgtgaaatc
     3901 agcgaattaa cctgagtatt agagaaaaaa atggaaaaat agtccctcat attttaccgt
     3961 ttgttgaact aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc
     4021 ctacccccca gctcctagtt acaaggacaa attcaaaaaa cagctcttca tattttatga
     4081 atgttgtact tgtgattcgg aggtggcaag ggaacactgt ttattcaaaa agaggctggg
     4141 acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt ttcaaatttt
     4201 tgtgaatttc ttcttatttc cgaaggagtt gcttctactt ccgccgttta tacgtgttta
     4261 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaatgtt tttaagattc
     4321 aactgaatag tttggtgctt cttttgtgat ttgtacatca tgagggtgtg attcttttaa
     4381 tccactgccg ctcatttgga taaattgcgc ttcatcacgt aattgctgta gattggctgc
     4441 cccaacataa cccattcctg aacgcaaacc gccgatcatt tggaaaataa tatctgcaac
     4501 acttccttta taagcaaccc gaccttcgat tccttctggt actaatttat ttgcttcatt
     4561 gacactacct tggaagtaac ggtcttttga acctttttcc atcgcgccta atgagcccat
     4621 acctcggtat gttttgaatc gacgtccttg gaagatttcg aattcacctg gtgattcatc
     4681 tgtccctgcc aacatacttc ctagcatcac tgcatgtccg ccggcagcta atgctttgac
     4741 gatgtcacca gaatatttga tccctccgtc tgcaataatc gctttgccat attgacgagc
     4801 aactgatgct gcatcataaa tagctgtgag ctgaggtaca cctacaccgg ctaccacacg
     4861 tgtcgtacaa atcgatcctg gtccgatacc aacttttacg acatctacac ctgcatcata
     4921 taaagctttt gtcgcttcag ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa
     4981 agttgaacgg atttcttgaa tttttcggat cacacctgca ctatgtccat gagctgtatc
     5041 gatgatgatt gcatctgccc ctgcttctaa taaagcattc gcacgctcaa atgtatcgct
     5101 tgttacacca acggctgctg ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg
     5161 aaattcgatc actttctcga tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt
     5221 atctacgatt ggtaattttt cgattttatg tttttgcaaa atcttttctg catcttttag
     5281 agaggtacct actggggcag tcactaagtt gtcttttgtc atcacatcag caatcggcat
     5341 gtgataatct gttacaaaac gcatgtcgcg gtttgtaatt atccctacca attttctgtt
     5401 ttcaagtgtt tcaacgattg gcacaccact gatacgatat ttgctcatca attcttctgc
     5461 gtctgctaca agatgctgtg gcgttaagaa gaacggatca atgattacgc cactttcaga
     5521 acgtttgact tttcttactt catccgcttg ctggctgatc gtcatgtttt tatggatcac
     5581 acctagtccg ccttgtcttg ccatagcaat ggccatcttg ctgtcggtaa cagtatccat
     5641 acttgcactg atgattggaa tattcaactt aatattttta gctaattgta cgctcatatc
     5701 cacttcgttt ggtaacacgt gactttctgc tggtatcaat aagacatcat caaaagtaaa
     5761 tccttttttc gtgaactttg tttcccagtt ggacattttt agtaccactc ctctaattta
     5821 gtttatctat taaaataatt gattttttca tttaagtcaa tgatctactt ggaatcaagt
     5881 tagctttctc atagaatacc aatataaagc gtttacatct gtgtatatta aaagagattg
     5941 t
//
LOCUS       DARDHD010000142         5938 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000142 DARDHD010000000
VERSION     DARDHD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.142"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(261..770)
                     /locus_tag="SDA06_002206"
     CDS             complement(261..770)
                     /locus_tag="SDA06_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG4229463.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(971..1840)
                     /locus_tag="SDA06_002207"
     CDS             complement(971..1840)
                     /locus_tag="SDA06_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4229464.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1860..2981)
                     /locus_tag="SDA06_002208"
     CDS             complement(1860..2981)
                     /locus_tag="SDA06_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4229465.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2983..3378)
                     /locus_tag="SDA06_002209"
     CDS             complement(2983..3378)
                     /locus_tag="SDA06_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4229466.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3397..4203)
                     /locus_tag="SDA06_002210"
     CDS             complement(3397..4203)
                     /locus_tag="SDA06_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4229467.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4196..4984)
                     /locus_tag="SDA06_002211"
     CDS             complement(4196..4984)
                     /locus_tag="SDA06_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229468.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4998..5474)
                     /locus_tag="SDA06_002212"
     CDS             complement(4998..5474)
                     /locus_tag="SDA06_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229469.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5546..>5938)
                     /locus_tag="SDA06_002213"
     CDS             complement(5546..>5938)
                     /locus_tag="SDA06_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229470.1"
                     /translation="RHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAI
                     YILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQ
                     SFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1835 a         1286 c          857 g         1960 t
ORIGIN      
        1 aatttttcaa catgtcccag agtaaactct gatttattcg ctaaatactt tttttgttgt
       61 attaaagaca tctttccccg aagctcaaaa ttgagcctac atcgattctc tatctcttca
      121 aaaagcatct tatcttcatc attcttatat tcatcattca taaaaccaat ccagctcctc
      181 gattaagata atattttaat ctcatacaaa aactagctct catcaaaaca accattgttt
      241 tgatttctca tgaaatttct ttatactttt ctccgattat aaattcctaa ccccttgaga
      301 aaattacgca tatatttatc tccgcaaggc ttaaaatttc gttggccttc ttttctcaaa
      361 aatgccccga tttcgccttt tgatggatag atttctgcct cagcaaaaac gtctaaaata
      421 tcatcagtgg tcatagacaa ggcgattttt aattttttta gcaacacatt gttgatatat
      481 ttgatttcct tgcttttttg cggcgtgtct actatcggct tatctttttg cggaccacgc
      541 gccaagagaa tcaggccatt taggaatgcg tcaaaaactt ggttgttgat ggttttttca
      601 tacacattat tttctgcatt atctttgttc ttatcctgta ctttggtcag taccgcctga
      661 gcttcttctt tagtcagtgg tacaccgcct agctcaaaag ctttaatcaa atccgtatct
      721 ttcaaatcaa tcgcataacg caatcgcaat aacctatcat tgttattcat ttgttcctcc
      781 gaagtattta gtctttactt tcttacgtac tttttgtctt gctaagattt ttaggatcaa
      841 tgataaaaac cgcatcactt atgataaggc gctgggtatc atgagtgaat gatattgctg
      901 aatacaaata gtccgaccac attaagttga tcggacttca taagcatttt attgactaat
      961 acttttttgc ttaagaattg attctaacat cttttgctaa agttaagcat cctatagttc
     1021 ctgcattatc tgctaactta ggtgtgacaa tgtattcttc gatatttggc gtatcgacat
     1081 agccgttaat tagttctaaa aaacgtcttt gtactttctt tttcagatgt tcttgttgca
     1141 tgacaccacc accaaaaatt ataatatctg gtgaaagcat cagggtcgta ttacaggcac
     1201 attgtgcaat ataatccgcc tcgatttccc agaacggatc ttctatagaa agatcttgtc
     1261 ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc caacccttcg agacaatcat
     1321 gatgaaaagg acaagtacca gagaaagtat ctcctgaatg tctcttaact aacgtatgcc
     1381 ccatttctgg atggctaaac ccttcaacaa aaatcccatc ttgtatagct cctccaccaa
     1441 ttccagttcc aacagtgtaa taaacaacac tggataaacc ttttccatga cctgaaacat
     1501 actccccgta acaagcagca ttcacatcgg ttgtccaggc aattggaatt tcgaaatgct
     1561 tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt ttttggagta gaggtgatat
     1621 gtccataggt ctttgattca cgatggatat caatcggtcc aaatgaccct actccaatgc
     1681 tttctaattg ttctttatag tgattaaaga aatcaattac caaactcatt gtttcattcg
     1741 gtgtagttgt aggaaaactc actctttcta aaacctttaa atcatcattg ccaatggcac
     1801 aaacaaattt agtcccacct gcttctattg agccatacat ttcttactcc accgtccttt
     1861 tatctaggaa ttcgttcttc cctgtctaat agtttttcga aaaactcatg tggttccttt
     1921 tggtaccaat aggcaacaga agaaatatcg tcttgacgct cataaagctt gatgtcatca
     1981 tttcctaggg tttggaaagt aacacgtaaa ttttctttga aactaatggg gtcgggtaaa
     2041 tgccagcggt acaacccatg catcggtaaa gcatcatcgc caaaaccatg aactggattt
     2101 ggattaggtt ttccagcact aaaataatcc cgggtgtgat ctcttttaga ttgaaaagga
     2161 tagcccataa atagcgtatt aaaattttga gcatatgccc tttctccaaa gttcttttta
     2221 tggaaagccc aagcgccgcc aaaataatct tctgctcctg tagatgttat tgtagggaaa
     2281 tcttcatctc catctatata aaacttaaat tctccttccc cccaccagta tctttccaat
     2341 gcgcacaaag caaagtaggt tcctacataa tacccagtgc ctttgattcc atcaataagg
     2401 gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc atttagcatg aaaatacatc
     2461 gcatcatccg gtacttcagt caatgcataa ttaaatgtat aaaaaatgtg cttaataaac
     2521 tcaggatgtt cattcgttaa agtaatctta gctttttttc gaaatggcat ctcaaaatag
     2581 caattcatcc ctgatgttgg attcactacg attggtaagg aattcacttc gcatcttgtc
     2641 ccaaacccat tacaaaaaaa atctcctaat ggactttcta cagaaggagt ggattcatca
     2701 tcccaataaa tacgcaaaat taaatcacgt aagacgaaac tcccagcgat tgttttgtct
     2761 gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt ccatttctgc taaagtaatt
     2821 gtctctccag catttatcgg aacagaaggt gacccttttc tcccaattcc tagatgactc
     2881 gctgtcattg ccgctttccc tttttcacca tttttatttt ctggtgaaat agttctactt
     2941 tcctctccct tgtggagcca tatattattt aaacaattca ttttattcgt cctctatcct
     3001 ttcttcactt ttttgattat tcatatagat gatttgttgt tgtgcctccg caataattgc
     3061 tccaatatct aaattatcat ttcctaactg caaagcgatt gacattgcca agggaagatt
     3121 cattcctgca attacgtgca ctctgtcaga aatataggta aaaaactttt gatttacact
     3181 tcctccagcc atgtcagtta aaacaattat ttcgtctgta gtatccacta actctttaat
     3241 ttgttcatct atatcatcaa tttcttcgac atatgcacat aagctactaa tttgattatg
     3301 catacctgtt aaaaactcta gtgtctcttt aaaaccctca gctagtattg aatggctagc
     3361 aatgatcaat cttctcataa ttcaaatcct tttcctttac gctaatattc caaatgcagc
     3421 cataaccatc gcaaaaacaa taacgagtag aatcaaaatg gtcatactga tttttttctt
     3481 actgagtaga taatatgctg cagcagtaat aattactgga attaatgttg gtaaaatttt
     3541 gtccaacatt ggttggattt tcatactaac ttcaccaaaa gtgaatttaa gtggcgttga
     3601 aacactgata actgaagcaa ttaatgatcc aactactgtt agccccaata ctgaagcagc
     3661 ttcagttaaa taattaatct tttcaccgta ttcagtaaca atcttcattc cctgcttata
     3721 accaaattca aagcctctca ttcggaaaaa gaagattacc atgattaaaa tgaaccataa
     3781 aaagattcct acaggattat tttctaaagc catataagcc gcaattgatc ccatgatggt
     3841 tgggatcatc gtcatcaaaa gagagtcccc aataccagca attggaccca tcgtaccaac
     3901 tttaaagtca ttaatcgcat cttttgcttc taatccttgc ttttcctcca ttgcaacaca
     3961 agcacctaaa ataatggctg ccagccaagg ctgtgtattg taatactgaa agtgattatt
     4021 taaagcttcg atgtattcat catcatcttt gtataatttt cttaaaatag gtgacaatgc
     4081 gtaagtcaca gatgcacctt gctgtgtttg ataattaaag gtacaaactg acatcaacca
     4141 acgatagttc gctttcctaa gatccttttt cgttacttga tatttttcgc tcattttatt
     4201 catcctcccc aataaaccca acatcagaag ctcccgcaga agcagtgatt tgtgcatctt
     4261 tttcagctct atgatacaca ttaaatgcta aagcgaatcc tatgatcgcc gctccaataa
     4321 taggtacctt caaaaaagcg gctaagaaaa atccaacaag taaataacta atgtatcgac
     4381 ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc aggcaacatt tttccggcta
     4441 atgttagccc atccataaac caattaggta aaacatccaa aacgtaatta acagcagact
     4501 gtcctaaagt aacggcaata aatactggaa ttgctgcaga taaaccaaag aaaattggcc
     4561 ccaaccacaa aacactattc attttcttaa attctcgttt attcgcatat tcctgtgatt
     4621 tgcgaacaac aaaaccattt aagattttga caattacatc tagttgcacc cctaacatac
     4681 ctacaggtag tcctattgct aatccggctt ctactccaag ctggctacca aatgccgttg
     4741 caataattgc gcacgttcca tagtccggta cagatgagcc accaaaaccg gcaactccta
     4801 atgacatcaa ttgaactgta ccgccaacta tcaaacctgt ttttatatca cccatcaaaa
     4861 ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa gatcatcgga atcccataat
     4921 aatcaacatg tttaataaat gctaatatag tcagcataat aatttgaaaa atagtaaaat
     4981 gcattatcct tactccttta tcctaatttt gattgaattt cctgtgcctt atccgccgga
     5041 acaaattgtg taacaatttc tacaccagaa tccaacaatt ttttatacgt gctgatattt
     5101 tcatctgatg cataggctct agaagatagc tgtaatgcat tatctttaac aacagtaccg
     5161 ccaacaatta gtcggtcaat tctaataccg ttatctacta aatgaaggat tgtttccggc
     5221 tctttcacga tgacaaatac tttttcctga tcgtatttcc cctttttaaa attcgcaatc
     5281 gctgtttctt cagtgataat tgatacagcc attccagcag gtctagctaa acgcatactt
     5341 gatttttgga tgtcgttatt tgcgacagaa tcatcgatca ccatcactct gtttgcttta
     5401 gacattggtg cccattgcgt gacaataatc ccatgcaata aacggttatc aactctcgac
     5461 ataacaatac tcataaatac ttcctccttt atctttcatt ttcttacaag caattaccat
     5521 gccaaaacaa aaagaacttg ataaatcaag ctctttttac ataatcataa acgtatttaa
     5581 cctcatgaat cgggatgtcg acactataat aatgttcaac gacactgaaa gattgcttaa
     5641 aatgtgtctg aaatttttga aattcctcac tcgtttcatt gaaataaatt tcatcttcca
     5701 aagtattttg ttttactaat cgttcaatta agcaactaat gtgaacatat aatccgaaac
     5761 acgtattatt atcaaatgaa attcctagat cattttgtaa gatgtatata gcatctgaaa
     5821 cccgttctaa taatttagtc ggatttaata tggtcaattg ctccattaaa ttcgttagac
     5881 taaaattctt cagaatatta ttgttcaaaa ctttcagttc attatcatta aagtgtct
//
LOCUS       DARDHD010000143         5929 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000143 DARDHD010000000
VERSION     DARDHD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.143"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..916
                     /locus_tag="SDA06_002214"
     CDS             191..916
                     /locus_tag="SDA06_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229471.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            906..1415
                     /locus_tag="SDA06_002215"
     CDS             906..1415
                     /locus_tag="SDA06_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229472.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1485..2933)
                     /locus_tag="SDA06_002216"
     CDS             complement(1485..2933)
                     /locus_tag="SDA06_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4229473.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2933..3649)
                     /locus_tag="SDA06_002217"
     CDS             complement(2933..3649)
                     /locus_tag="SDA06_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229474.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3630..3980)
                     /locus_tag="SDA06_002218"
     CDS             complement(3630..3980)
                     /locus_tag="SDA06_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG4229475.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4124..4897
                     /locus_tag="SDA06_002219"
     CDS             4124..4897
                     /locus_tag="SDA06_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4229476.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5068..5434
                     /gene="ssrA"
                     /locus_tag="SDA06_002220"
     tmRNA           5068..5434
                     /gene="ssrA"
                     /locus_tag="SDA06_002220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1740 a         1153 c         1019 g         2017 t
ORIGIN      
        1 tattgtcgct gctcctcttt tcgaataaac agtgttcaaa agcttgcatc tacggtaata
       61 gaatagggca gcttcttcac aacctcttgc tttttacaaa atattttctg gttttaattc
      121 gtgatttcac taaaggaatt tcatacttta ttcatattca ttttctatac tttttataga
      181 ggtgaaaagt atgaaaataa tccgctatgc tactgctcga ttaatttatt acaaaaagca
      241 gacaatactc tattgtttat tttcaagctt ttctgctttt ttattgatag catgcctgac
      301 tctttaccat ttgcaatcgg ctttagccaa tcaagtccaa gatcggcttt catttattgg
      361 cgcttctgca tctacagagc ttcttccggc tttagaatta gctagccgat tttatataaa
      421 aggcatattc agtttatttt ttctatttgc cttccttttt atctttttct tttactattc
      481 agtcaggcaa aacaagcagg aattgatcaa ttggcgcctg actggtcttt ctaaaaccaa
      541 attattcctt tttattttct ggcagctgtt ctttcctctc tttctttgtt gtatcttggt
      601 tttattgtgg atcgtaagct tccagtcagt ttatgaagcg cttcttcaaa ggatcaattt
      661 tcttgtctta aagactactg aactgccaga tatccgtgat atcttgactt cggtaagcgg
      721 acttacaatt ccgatggatc aacagacttt ttttaccatc gatttccaaa atgatctttt
      781 tttcacagat atacttaagg gatttgccca aactcttatt gggctcagtg gatgctgtct
      841 cattcttagt ctattccctt tcactttttt tatccatcat ttgcaaagta ggaggcctgg
      901 acgtaatgaa acctgaatat ttgattcatg ctgaacactt gaccgttatt ttgtgtcctc
      961 tcgaaaaaca gcataaaata ttggagcact tgaaaaaaga ggttcttgtt gcaaaactgg
     1021 ataattttgc gatcatccaa aaaaattggc cgatgtttcc ttatctgaat cttaaagacc
     1081 atgtcctgtt ggatgtaccg gaaaaagaaa tcaaacaaga tcgtttggcc tatcaagaaa
     1141 aacttgatat ctctccttcc ttgcttaatt gtgcggcagc tgagttgacg tcttttgaaa
     1201 aagtaaagct gcaattactt catgccttgc tttccaagcg aaacaatatt gttattgaag
     1261 atacatttga tgaacttagt gtattggaga tccaagagct actccacttg ctttcttatt
     1321 tagctcatga agaaaatcaa ggtatcttac tctttactca tgatgctact attgctcaaa
     1381 gtccttatat tgatcgttta gagcccgcgg gctaaatcaa tcattttata gtaaaaacaa
     1441 gcggtataga agatcgattc atgatctttt ataccgcttg ttttttactc ttttttgatt
     1501 tctacgtcag gcaaacgaac agcgaatttt gttccttttc ctagttcgct ttctacttct
     1561 aattttcctt tatgcaagcg tacaagttgc tgcacgattg gtaaacctaa tcctgattca
     1621 ccatattttg tattttttct tgaaggatct actttataat aacgatccca gatacttttc
     1681 atttgttgtt cagacattcc aattcccgta tcttcaatcg tgatgatcgt ttcaagatag
     1741 cctttttcta aagtaatcgt gatttggccg ttttcagtaa actgtatcgc gttttgaata
     1801 atattgacca tgatttggac aaatcggtca taatcagcgt aaacatcaat atcatcaacg
     1861 gctttcaatg tcaatgtatc tcctgctgct tctgctttag cttctaactg tgcaacaata
     1921 tttttcaatg tttctgttcc attgaatttt ttaatgacca tactgatttg attcgtacgg
     1981 attttttcat aatccaagtt ttcattcacc aaacggatca gacgctctgt ttcatttttc
     2041 atcaatttaa tcgcgttttc tcgctgattt tcagggattg cattgtattc taacccttcc
     2101 agaagtccgt tgattgtcgt aagaggtgtt ctcatctcgt gagaagcatc tgccataaat
     2161 tgtcttcgac gattttcttg ttcttcaatt tctgcttgag attctttcaa tgaattcgtc
     2221 attttattga aatcatctgc taactcatca aattcatctt tgtcatgtac ggggagctgt
     2281 acatcaaaat taccattcgt cacttctttc gtcgcattcc ttaaccgatt gatacgctta
     2341 acttggaatg ctgcaaaagc atagctggca ataactgcaa cgatactgga aaaaataaat
     2401 cctttgaaca ggttcagtgt aattggacga acgctgttat cgatatttcg cgcaggttga
     2461 ctaactacaa gtgctccata gaattcatga ttcaaattaa atggcacaag tgcgtaagag
     2521 gtcgcctgtg cttgtcctaa aatattttta ttcgatgtga atttttcttg acgtccattc
     2581 ctcagatttt tccattgatc agctgttaag gtaaaattca gtaaagctgt attagtcgga
     2641 taaatgacac gttcgttttt atcgataaat ataaagttca cattttgttc tgtcagtact
     2701 tgctctgtca gcagcaatgc attttgaaac gcctgatctt gactaaactg cgggagcgta
     2761 tcggcatagg tttgcgttgt cttttcaact gattcagcat atccaaacag ttgctggtaa
     2821 ttgttttctt caatcgtttg ttttgtcagt tgtgtaaaag aaactccaac gatcaataag
     2881 atcaacgcga ccattccgca aaaagctaag agttgctgat acaaataacg cattaggcaa
     2941 caccagaatc atcgaatttg tagccgactc cccaaacagt ttggatgact tgtggtccga
     3001 ccttttcgat tttttgtcgt aatttcttga tatgggcgtc cactgtccgt tcatcaccaa
     3061 agtactgata atcccagact aattcaagca actgctcccg agagaatact tgacgtggtt
     3121 tttttgctaa agtaaacaat agatcgaact ctttaggcgt cagtccttca attggccggt
     3181 tgtccagata cgcttctcgt gttttcgtat tcatcttgaa atgactagtc tcgatatcaa
     3241 aatgatcatc tgaaactgct gcttcttctc ttgcttctgc caactcacta cggcgatgca
     3301 aagctttcat tcgagcaata agcgtcagag gactgaaagg ttttgtgaca taatcatctg
     3361 cccccatctc taagccgatc acctgatcgc tttcagaatc tctagctgtc agcatgataa
     3421 tcggcacagt acttgaaact ttgcggatct ctcttcctac tagcataccg tccagtgtgg
     3481 gcaaattcag gtctagtgta atcatatccc aatgttgtgg gttttctaaa aaggcatcga
     3541 gcccttcttt tccatcatac ttgaaagttg cttcccaacc ttcatttaag aaaaacatct
     3601 gcatcatttc agaaacagat tgattatctt caatcattaa aatattcatt atcgttccct
     3661 ccagtttcca ggtcatattt ctgtaatttc ttttctgtca taataaaaat aggcataatt
     3721 ggaaatacta gcagaagcat ttcaatcaga aaataattcg tatgcccaga tgtctttaaa
     3781 taatagccga tcaatgccat cagtaagccc ataagaaaaa accagtttcc gcttgtcttt
     3841 tgagccagcc gccaatgagc gtctgttttt gcggcaagag gcgaatgata cccataaaaa
     3901 ggcagatttc cttttgaggg cagaatccga aaaagactgc caattattaa gaataagata
     3961 ccaatactga aaaaaatcat tctttccctc ctaaattctc ttcttatagt atactctaaa
     4021 ttgttgactg aaccaatatg ttcaggaatt cttcacgaaa ggtttgtctt ttgttcattc
     4081 tttgttaaga taaacttatt ccaataaaaa aggagtttta tttgtgtcaa attatcgtgc
     4141 aatcactttc tttgatcttg atggaacatt actagatgcg aaatcaaaaa tcacacccga
     4201 agtggcaaaa gctatgaacg cattaaaaga aaacaatgtt ctccccgtca tcgctacagg
     4261 acgtacagaa gcagaaatcc actctatcat ggaagatgcg ggcattactt ctgcggtcac
     4321 catgaatggt tctttcattc gagtagacgg cgaagaaatc tattctcaaa cattttcttc
     4381 agaagaatgt caaaaaatgt atgatcatgt acagttgaac ggtcatgaac tttcttacta
     4441 taacgatcag aaaatctggt gtaccggtca tagccaaaca atgatcaatg cttacgactt
     4501 cattcattca gatgttccgg aaattgatcc tttaggcttt aaagaaaaca cagtaaatat
     4561 gttgctgatc ttatctcaat caggtgacga atactatcac gaacattttc ctgagctaac
     4621 tttttatcgg aatggtcctt actcgatcga tacagtgaag aaaggaatct caaaaggggc
     4681 aggtgtcaaa cgtctgcaaa aagaacttca gctggaaaaa gttcctactt ttggatttgg
     4741 cgatggtccg aatgattttg ccttgttgga agcttgcgac aacaaaatcg ctatgggcaa
     4801 cgcctatgac ggattaaaag aactttctac ctttatcaca aagaaaaata cagaaggcgg
     4861 aattgtccac gcattgaagt attttgatct gatttaagat ctttttttga agctacctct
     4921 tttcttaaga aaatcgctta ttttttctta agaaaggggc agttattata taaataatgg
     4981 gatatgattc gcttttttat tgaatttatc ccaatatatg ctatactaat tttgtatgaa
     5041 aaatacacgt aacccttgtt agctaacggg gacgttacgg attcgacagg cacagtcgag
     5101 cttgaattgc gtttcgtagg ttacgtctac gtaaaaacgt tacagttaaa tataactgct
     5161 aaaaacgaaa acaactctta cgctttagct gcctaaaaac agttagcgta gatcctctcg
     5221 gcatcgccca tgtgctcgag taagggtcct aactttagtg ggatacgttt caactttccg
     5281 tctgtaagtt gaaaaagaga acatcagact agcgatacag aatgcctgtc actcggcaag
     5341 ctgtaaagtg aatccttaaa tgagttgact atgaacgtag atttttaagt ggcgatgtgt
     5401 ttggacgcgg gttcgactcc cgccgtctcc atatatgtat ggttgagaca gataaacatg
     5461 tatgaaaaac gttgttaaat caacgttttt ttattttggt caaggagaga aaagtagaga
     5521 aatacaaaat tttttgcatt gattttgcat tcaaaaaaac attcaaacat ttagaaatcc
     5581 ccaatacgta ttcaggaaaa tctattttaa ggtttacaaa aagaactttt gttcgtacac
     5641 tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaagcatg
     5701 ttccttcaaa taagtttttt aattataaac tctagagaaa ttaattcatc ctaacataaa
     5761 ataattgttc actcaaaagt ttttctagaa caattaaaac agcaggcttc acattcgcta
     5821 tacatttgta agaagtaata attcctgtac taaaaggcat aattgcttta attatttttt
     5881 gacttctctt caaacacaag aagtagttgt gtaataattg cagaaataa
//
LOCUS       DARDHD010000144         5911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000144 DARDHD010000000
VERSION     DARDHD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.144"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..610)
                     /locus_tag="SDA06_002221"
     CDS             complement(<1..610)
                     /locus_tag="SDA06_002221"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEG4229477.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIAS"
     gene            complement(694..2121)
                     /gene="uxaC"
                     /locus_tag="SDA06_002222"
     CDS             complement(694..2121)
                     /gene="uxaC"
                     /locus_tag="SDA06_002222"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4229478.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2195..3274)
                     /gene="uxuA"
                     /locus_tag="SDA06_002223"
     CDS             complement(2195..3274)
                     /gene="uxuA"
                     /locus_tag="SDA06_002223"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4229479.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3277..4908)
                     /locus_tag="SDA06_002224"
     CDS             complement(3277..4908)
                     /locus_tag="SDA06_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4229480.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5225..5896
                     /locus_tag="SDA06_002225"
     CDS             5225..5896
                     /locus_tag="SDA06_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4229481.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         1863 a         1189 c          833 g         2026 t
ORIGIN      
        1 tactagctat tcgagttact tcttcaaaat cacctttttc aacaggagcc aacaaattcc
       61 cacctacacc aactgcaaca acgcctgctt caaaccactc ttctaagtta tctagactta
      121 ctccgccagt aggcattata tttacatatg gtaatggagc ttgatacgtt tttacaacag
      181 atggaccaaa agcagaacct ggaaatagtt taatgatttc tactccagcg gtaagtgcct
      241 cctttatctc agtaattgtc atacaacctg gtaaataagg aatttgatat aaattacaaa
      301 tctctgctgt ttgtcgatca aaagatggac ttacgatata ttctgctcca gccataattg
      361 caatacgagc agtaaacgca tctaaaactg taccggctcc aattattaca tctttttgat
      421 cagcatattt tttagcaatt tctttgatta tttcttcagc attcggaact gtaaaagtta
      481 gttcaatccc tttaattcca cccgcagtaa ttgcatttat tgctttaatg gcctcatctt
      541 tattttttcc acgtacaacc gcaatgactc cagccgcttc taacttactt aaaatttcta
      601 cttttttcat atctatctcc ttcttttatg gatacatact ctagctagtt gtactaactt
      661 acaataaata aatttaagct tttatttttt gtattatttt ctactttcga tttcaaaaaa
      721 attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac taagatacat
      781 ttcatcatct ggaattcgac ctgctttaac ccactcgctt agtaattgac ataatactcg
      841 acggaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca acatacctgt
      901 aaaattcgac aacaaacttt gttgcgccat cgtagtcaac tgttgacgca ttccttcagc
      961 tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac ccccttggaa
     1021 gctttgcatt gctgttgcta gtggaagcca atcggttgga tttaatgaat ataaaattgt
     1081 ttttggtaag cacccactct tttcagcagt gtcgaacaat gctacaacat tttgtgtcaa
     1141 actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt cctttaatat
     1201 acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat atcttgtata
     1261 ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg ataaatggaa
     1321 atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt catctgctat
     1381 ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt gatgaaaata
     1441 atccactcgt tggctcaatg cctgcttcaa cccattaaag tttttgattg gtatacccgc
     1501 tgcttgacta agttttaaaa tatagttcga aaatcctgat gatttgatac tccagacctt
     1561 atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa gctcagtatg
     1621 atatcgcaag tcatctgtag gatcatccgt tgtacaaatg gctttcacat ccatcatctt
     1681 cagcaattct tttgggcgat aggaatccgt ttggattttt tcgttggcac gtcgccaaat
     1741 ttctggtgca ttcttctcag aaattgtcaa atcaatatca aagtaacgtt gtaattccaa
     1801 atgagaccat tcaaaaatag gattacctgg cgccttctcc actgttttta caaaagctaa
     1861 aaatttttca taatcattac ctgcaccact gatcaactct tcttctatcc cattagctcg
     1921 catcaatcgc catttataat gatctccagc tcctttatca tttaaccata cctgagtcaa
     1981 attcttgtat tgcttatttt ctagcaaaca tgctgggtct aaatgacaat gataatcgat
     2041 gattggttgg tgttctgcaa atttatgaaa aagtctttta gctatttcat tctctaataa
     2101 aaagttctta tctaaaaaca tatttaagca ctcctttcct tcccttagtt tttagaagag
     2161 aaaatttttc ttctaaatag actccattat ataattattt actcgccccc ttttcaagcg
     2221 cttcccaaat accatttaaa taaactgcac ctagagctcg gtcatataac ccataaccag
     2281 gtctaccggt ttctccccaa atcatccgac catgatctgg acgaatatag ccattaaatc
     2341 cattatcgta cattgttttt aatatcttat acatatctag agaaccatat tctgatttat
     2401 gcggcgcttc ataaaaatcg agtccattat cacttaaata tttgatatta cgaacatgtg
     2461 caaaaggaat ccgatcccgt tgacaaaatt cagatagaat ttcatagaca tcattatctg
     2521 gtcgttctgc aatacttcca gtacataacg taataccatt agaaggagaa tccactacat
     2581 tacacaacca ttctaaatcg tcacgatttt taacaatacg aggtaatccg aaaatagaat
     2641 atggtggatc atctggatga actgccattt taatcccaac ttcttcacat gtcgggataa
     2701 ttgctttgag aaaatatgca aagttctctc gcaatttcgc ttcatcaact tctttatagg
     2761 cttctaataa ttgcttgatt tgtgataacc gatgaggttc ccaacccggc aattcaaaat
     2821 tattagtaga attttctact tttctaataa tttcctctgg atcagtagga atattttttg
     2881 aaatataggc taacgtagaa gacccatcat ctaaatgata atccaaatct gattttaccc
     2941 aatcaaagac tggcataaaa ttataacaga taacctcaat cccaaattct gccaaattgc
     3001 gaatcgtctg tttataattc tcgatgtatt gatctcgtgt tggcaaacca attttgatat
     3061 catcatgaac attaacactc tcaataactt tcaaggtaag gccattggct tcgacttcat
     3121 cttttaaagc tttaattcgt tctctcggcc agacctctcc ggctggtata tccattaatg
     3181 ttccaacaat tcctttcatt gctgggatct gacgaatttc ttgtagtgag attttttctt
     3241 catttgcccc ataccatcta aaagtcattt ccatagttac tttcctccaa cttctgttat
     3301 atatttattg attgttttac gaactgcttg aggacctact agcatttcgc aaaagatttt
     3361 ttcaattttc tttcctatct caacttgata taaatctaaa ccaaatattt ccttattttc
     3421 taagatttcc tgtaccgcac aatgaatctt tgcaggatct tgttcaccta atttcaatac
     3481 tgagaatttt tcctgcaact ctaccaataa aggatcagga cttggcacaa aattttttcc
     3541 ttgatcatcg actgccaata aataacgtaa ccaagcagca atagcgatag gaataaatgt
     3601 tagattttct gcatttccgt ctttttcgac atacttttta attgtttcac catagcgaat
     3661 cgctactttt tgagaagtat ctgttgcaat tcgttgcggt gtatctggaa tatagggatt
     3721 aggcaaacgt ttattgatta cttcatcaat aaattgtttt ggatcaataa cgcctggact
     3781 ttcaactact ggtaagcctt ctacataacc tatcccttta attaagccaa ccaaatcatc
     3841 atcttgcatt tctgcctgaa ttgatttata ccccaacaaa caacctgtta ttgctaatgc
     3901 cgtatggagc ggatttaaac atgtcgtaac cttcatgata tctgctttat caactgtttc
     3961 tctatctgtc ataatcacac cagccgcttc cagtttaggt cgaccatttg gaaaactatc
     4021 ttcaatgact agataatgga ctttttcagt attggtaaaa gcagcgatat tggtattttt
     4081 cgcagtatga attatatcta agtcttcgaa acccgccttc aataaatcca ttttgacact
     4141 ttccgaagga tttggcgtaa ttctatcaat catactccaa ggaaaagtca ctttttcttt
     4201 atcctttaaa taagcaataa atttttcaga aacgtatcct tttttttgcc aagcttctgc
     4261 aatcataaaa atgctatctt gaaaacgttg accattctgt gagaaattat cagtagaaac
     4321 catcgcaatc ggtaactctc cagcattaaa cctgctatac aacaaggatg tcacaatact
     4381 catcgtatgt tgaggattgg taggtccaga ttttatatct gcttccacta ccgaaaaaaa
     4441 ttgtccttta gaatctttta aactgtatcc tttttcagta atagtaaacg ttgtaaactg
     4501 taatgtagga tcttcaaaat atttaaccac tctttgatag tccgttatat tctttggatt
     4561 gcaatataaa ctatcagctg ttactgctaa aagtcttttt ttgagttcac catcttcacc
     4621 cataacaatc tccaaaatat cattgttgta gtcatgataa gctttttcta ctacttcttc
     4681 atcaaatgtc tcacacacaa ctactccgga ttctaattca ttttgatctg ctaagcattg
     4741 agcaatttct gcatgaaatc ctcgataaag atttcctcca ccaaaatgaa gccacactgg
     4801 atgtttctga ccttcttgtt tgaccgcttc gacattaaaa tacggtagtt gaacttgcat
     4861 tttgttcaat ccttcgatag aatcttgcca gtctttaatc aaaaccatat ttacccctcc
     4921 atactgatat gttagtttcc gttacaattg tagtataaaa tcgaatgtaa tcgctgtcaa
     4981 taagaaaaaa ctaaaaatat tcttttttct accatgtcag aattattgtg tcttttttta
     5041 gtaaaagcac accaatatat attttttaga actaacttaa attaaagctt aatttctctt
     5101 tgatcttatt tgagtatttt ttgttttttt aatagctagt atgttacttt gaaatttgat
     5161 attatataac gagcatagaa ctaaaatatc aaattcaaaa atgaaatttt gagagggatt
     5221 aaatatgaca attaaaacta gtctccaaaa tcaagcttat caatctattc gtcagcaaat
     5281 tatctacgct gacttagaac ctggaaaaaa agtatcagaa aaaaaattgg aagaaacgct
     5341 ttctatcggt cgtactccaa tacgtgaagc attgattcaa ctccgtcatc aaggacttgt
     5401 tgatacaatt cctcaaagtg gaaattatat ctcattaatc gacttagatt tagcaaaaaa
     5461 tgctcgtttt gtcagagaac atttagaacg acaaattatg ctagagtgtt gtgccaagat
     5521 gaataatcaa aagaaacaag ttttagaaac tataattgca gaacaagaaa aagctgttag
     5581 tcaaaagaat gctcgtagtt tttttcataa tgataattta tttcataaag cttgttttga
     5641 aattgcagaa cgggaagaag tatggcaatg gttggaagat aataatacgc acttggaacg
     5701 atttcgttgg ttacgagtaa aaacggaagg attaaaatgg gaaaaaatta tggaacaaca
     5761 tcatcaacta tttaaagcct tgaccatgca agaccctgaa gaagctaact tcttaacatc
     5821 cttgcatctt catttaatgc ttgatgaaca aaatgttgta ttgactactc acccaaaata
     5881 ttttaaaaat atataactgc taaatttctt t
//
LOCUS       DARDHD010000145         5891 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000145 DARDHD010000000
VERSION     DARDHD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.145"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(103..588)
                     /locus_tag="SDA06_002226"
     CDS             complement(103..588)
                     /locus_tag="SDA06_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229482.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(727..942)
                     /locus_tag="SDA06_002227"
     CDS             complement(727..942)
                     /locus_tag="SDA06_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG4229483.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1166..1966
                     /locus_tag="SDA06_002228"
     CDS             1166..1966
                     /locus_tag="SDA06_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4229484.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1985..3853
                     /locus_tag="SDA06_002229"
     CDS             1985..3853
                     /locus_tag="SDA06_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4229485.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3846..4337
                     /locus_tag="SDA06_002230"
     CDS             3846..4337
                     /locus_tag="SDA06_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG4229486.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4325..4879
                     /gene="srlA"
                     /locus_tag="SDA06_002231"
     CDS             4325..4879
                     /gene="srlA"
                     /locus_tag="SDA06_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG4229487.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4897..>5891
                     /locus_tag="SDA06_002232"
     CDS             4897..>5891
                     /locus_tag="SDA06_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG4229488.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2057 a          943 c         1188 g         1703 t
ORIGIN      
        1 taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa atgataccaa
       61 taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg gtgttccatt
      121 tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa cgcttccaga
      181 tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc gatcaatttt
      241 ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg caataagaaa
      301 tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt cttgcatttc
      361 agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg aatctacatt
      421 accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac caacctcagt
      481 tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc cacaggcaga
      541 aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga tagctcctcc
      601 taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa
      661 tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt taataattat
      721 agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt tcaagcagta
      781 gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg ttgaaccacc
      841 agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt tcaaccctcc
      901 cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc tcctttctta
      961 aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt
     1021 tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta
     1081 gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta
     1141 agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt
     1201 aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca
     1261 gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact
     1321 atctttaaaa gtcgatgtgt cttctaaaac ggacataaaa gaagggatat ataaagtaat
     1381 gaaacatttt aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct
     1441 tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa
     1501 gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt
     1561 ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc
     1621 agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg
     1681 ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga
     1741 agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag
     1801 cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg
     1861 taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat
     1921 cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac
     1981 gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa
     2041 agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga
     2101 tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca
     2161 ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa
     2221 aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat
     2281 cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg
     2341 aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat
     2401 tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat tacgactact
     2461 tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga
     2521 aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct
     2581 tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa
     2641 gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa
     2701 tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt
     2761 tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg
     2821 cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga
     2881 gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt
     2941 tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta
     3001 ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa
     3061 agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca
     3121 tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat
     3181 ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga
     3241 acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa
     3301 aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt
     3361 aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt
     3421 tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc
     3481 tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga
     3541 atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct
     3601 atctcaacac attgttttat cagtagggct aataactgat caaggtttag cagaaacagg
     3661 aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa
     3721 tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa tagatgagtt
     3781 tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag
     3841 acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag
     3901 cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa
     3961 aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc
     4021 tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt
     4081 tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt
     4141 accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag
     4201 aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta
     4261 ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca
     4321 aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc
     4381 agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc
     4441 aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc
     4501 agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg
     4561 aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta
     4621 tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg
     4681 agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat
     4741 ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat
     4801 aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt
     4861 agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta
     4921 agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag
     4981 ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg
     5041 acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt
     5101 gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca
     5161 accatcaata tcgttcccac aggaaaaagt ggcccattag cacaatatat cactgaagat
     5221 atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac
     5281 aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag
     5341 ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga
     5401 aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca
     5461 gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc
     5521 ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc
     5581 attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt
     5641 agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa
     5701 ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc
     5761 ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt
     5821 tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga
     5881 ttatatagct g
//
LOCUS       DARDHD010000146         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000146 DARDHD010000000
VERSION     DARDHD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.147"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SDA06_002233"
     CDS             450..1604
                     /locus_tag="SDA06_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG4229489.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SDA06_002234"
     CDS             1598..2020
                     /locus_tag="SDA06_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229490.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SDA06_002235"
     CDS             3143..4075
                     /locus_tag="SDA06_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229491.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SDA06_002236"
     CDS             4092..4607
                     /locus_tag="SDA06_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4229492.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SDA06_002237"
     CDS             4903..5184
                     /locus_tag="SDA06_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229493.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SDA06_002238"
     CDS             5186..5602
                     /locus_tag="SDA06_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229494.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARDHD010000147         5780 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000147 DARDHD010000000
VERSION     DARDHD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.149"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..958
                     /locus_tag="SDA06_002239"
     CDS             119..958
                     /locus_tag="SDA06_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4229495.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1109..4126
                     /locus_tag="SDA06_002240"
     CDS             1109..4126
                     /locus_tag="SDA06_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4229496.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4190..5653
                     /locus_tag="SDA06_002241"
     CDS             4190..5653
                     /locus_tag="SDA06_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4229497.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2211 a         1006 c         1200 g         1363 t
ORIGIN      
        1 gtaccaaagt tagtatatca aaaaataaaa aaatctacaa aaaatatatc gactgataga
       61 cataagtttg gtactatgtt tttgtaaata aatttattca aatcggaggc gaaaaacaat
      121 gaaaatgttg aaagtattgg cactaggaat cgtagtcgga ggagttagtc ttgctgtggg
      181 aacgaatgct gaagcagcag aaaacaaagt ttggacacct cgttcggcgg aacaaatcaa
      241 agcagaaatt aaagggaatg aatacacaat tgtctgggga gataccctaa gcgcaatcag
      301 tcaagcaaca aatattactg tccaaaaatt agcggacatg aataagatta caaatgtgga
      361 tttaatctat gcaggaaata aattagtatt taatggcaat gtagcaagtg tacaaaacag
      421 cgcaggtgaa actgtggctc aaacagtgat tcaagagaaa gacaaagttg atccgaatca
      481 accagtagga caaggaacga cttcaaatac acaaacgaca ccaaatacaa cgacaccaac
      541 aacaccagct ggagatcaaa caggaacaac tggtggtaca acaacgccaa gtacaccaga
      601 aaacaatggt ggcactactc aaacacctac acaaccaggt aatggtggaa atactggagg
      661 agaaacaact ccttctaacc caagcacagg tggaggaaca acaacgcctg aagaaccaag
      721 caatcctggt ggaggcgatc aaggcggaaa tggtggcaca acgacacctg aagagccaac
      781 aattgtagtt ggagagattg gtaatagtgg aaaggtattt aacactgtag atgaagccta
      841 tgattgggct aatgcagagg ttagcgatcc aaattcgcag tggtacaaca aaggttttat
      901 ggtggatgtt attatttaca gtgacgggtc acaaaaacac actgtagatt tctactaaaa
      961 ataaaagaaa agtaagtggt tgatgaagac tagaattata agttctagtc tttttttgtt
     1021 ttgaggagga taaaaatgtt tcggtgtcga agtccataag aaattacttt agaagactga
     1081 aaaataaaat gaaaggaacg aaaaaaatat gtttaaaaga atggcaactt ggttggcaat
     1141 tttcgttaca tttctcaatc ccatgttagg atctgtaacg gcatttgcag aaacgataaa
     1201 tgaatcaaat tctactgaag aagtaatgaa gactgaagaa tcaaaaagta gtgaagaagc
     1261 gatcagtact gaaaccacaa gtgaaacaac agaagagaca actacgcagg acacgacaga
     1321 agatgtttct ccgcccaatg taatgactca agaggtccct gaaggttatg agaaatactt
     1381 tataaccatt gaagatattc aaaaagcgac ttctttttct gaacaagaac gattggcgca
     1441 agaatctgaa gaatggcaag caatcttgga aaagaatgca cagacacgtg ctaccaatct
     1501 aacattgact gaaaatggtc gtaaagatgt gaatatgact cgaattactc ctgaaggaac
     1561 aatgtatact catgattaca tggttcgctg gcaaattgac ggagaagatg ttttttgcat
     1621 tcaagaaggc gcaatcacga gtgcgggtat tcaatatgat gtgacaccac tcgaaaattt
     1681 aattacgaca agtgctatta gaaaaaagat ttctttaatt ggttattttg gctactattc
     1741 tagtggttca atggataact atgtattagc acaaacatat atttgggaag ttttaggtgg
     1801 ttcatttgtt aattatggaa ctattggaca atctaattat gaagctttca aacagactgt
     1861 agatagaaaa ataaacaatt tacttgaaga tccttctttt gatagccaaa cagtgacggt
     1921 taaagtaggg gaagaagtag aagttaagga tacaaaaggt gtgttttctc aatgggatgg
     1981 gtcggttgtt gaaaacacag ctaacgttca agtaagaaaa tcaggaaata gtttgtttat
     2041 aaaagcaaat caaaactcaa atgacggacg aatttctgtt tcttcactgg atccaaattc
     2101 tagaggggta ggttcaagtt ttgcctatgt agatcctgtt gctcaaaatc taggaaagct
     2161 ttatgtgcca gatcctaata gagctggact aactattaaa gttctaaaaa atggcgactt
     2221 caaactaacc aaactagacg ctgtcacagg gaaaaaagtt ccaaatgtga cgtatgaaat
     2281 caaacgtcat gatacgggag aaacaaaaca atatacaacg gatgccaaag gggaaatcgc
     2341 cttaaaagaa atccctgcat tctatgaaaa agacggaaaa tatgttcaaa ataaattgga
     2401 tgtcaaagaa gtcaaagcac cagctgggta tattctagac aaacaaactc atacgattac
     2461 gattgaacca aacaaagaag tgacgttaac tgcaaaaaat gacttacaac agttcattcc
     2521 agaaggaaag aaagtcaaag aagtctataa tgcggaagaa tcctggaatc aaggcaaacc
     2581 tgtttatgat accgttcctg cagaaaatat cgaatttgat attatcgcca aaacagatat
     2641 tactgcgcca gataaagaaa cagtccttgt gaaaaaagga gaagtcttgg attcagtaaa
     2701 aactgacaaa gacggacact ggaaggcaaa caaaaaattc tacaatggcg atcaaaacaa
     2761 ataccaattg attgaaaaaa atgtgccaga caactaccgt caaccttctg aagaacaaac
     2821 cacgttctct gtggcctatg gcgataacac gacgcctgtg cttacgtttg aattagatgt
     2881 gattgacaac ttgttaaaaa cgggtgaagt agacttcaat aaagtcgatt caatcaatca
     2941 tctacaatta ccaggagcga aattcctgat tgaaggtatg actgcacata ataaaaatgt
     3001 gtcctttgtg tatgaaacaa aagaagaacc agaaaaacta aaattaccag acggtacgta
     3061 taaatttacg gaaattgtgt tccctgagca ttatgggcaa gcgtcaggac aaacagaaac
     3121 gattttagta acggtcaaag acggagaagt acaaccaatt actgtgaaaa acgaagaaat
     3181 cgtggttcca gaagaagacc aaccagaaat ttctactttg tttgtgacaa cgacaggtgg
     3241 aaaagatttc aacccgaaaa aagaccaaga attagtggat aaagtgtccc aagtattccc
     3301 taaatctgcg gttgggacag agaaatattg ggtgacgcaa ttccataaaa ttactgccga
     3361 tggaaaagtc actgtattag atacggtaaa agaaacacga aaagtgaccg atgaaaaaga
     3421 aacatttgat gtgacgttca actacaaagc agacatggtt tcagacggtg acacgattgt
     3481 cgcaacgcac attgtctata acgacaaaga agaggaagaa gaattcgctc gtcattttga
     3541 tttaaccaat aaagatcaaa cagtgactgc gaaagaacca aaaatcgaaa cacgctttat
     3601 tacagtgaat ggcgaccaaa catttgatcc agacaaagac caagagttga tcgatcatgt
     3661 atcgcaagaa tttccagaat ctacggtagg ggaaaccaaa tactgggtga ctcaattcca
     3721 taagattgat aaaaatggaa aagatacagt cgttggtacg gtagaaactg cacgtaaagt
     3781 ttctaaagcg tctgaagagt ttaaagtatc ctttgactac aaagcaggca cgttaaaaga
     3841 cggggagaaa ttggtcgcaa ctcatattgt ctataaggat aaagaccata aagaggaaga
     3901 agcacgtcac tttgatttga agaatgaaaa acaaacattg acagcaaaag gcattaaaaa
     3961 agaaacgccg acaacccctg tcaaacaaac gccgacgagc acaccaaaac aaagcttacc
     4021 aaaaacagga gaaacagtga acgatgtgtt aattgcattg ggactgtttc tttttgttgg
     4081 tgtgggggct gttctggttc gtcgtaaaat gaaacaaagc aattaaagta tgactagaga
     4141 cagcaatcag ctgtctctag atgattgatt gaagaatgga ggcaatcttg tgaaaaaaac
     4201 gagtaaatgt ttatctgctt tgttattagt aggtcaagta tttgggtata cgccagcggt
     4261 tttggctaat gaacaaggca acaatcaact gacaaattcg caatctgcaa cgatcactga
     4321 agaaacgaca acaaattcca ctggagaaga aaatcaacca gaaacaccga ctgaagatac
     4381 aatagataca ccagataatc cagaaatgaa cgctccacca acaggttctg aagaacaacc
     4441 agaaacaccg aatcaaccag acaaaccaga agacaatcaa caacctgagg aggaaccgtc
     4501 agaagaacca aatgagcaac cttctgatga aagctcacag actcctcaaa aaccaagtac
     4561 cgaaacagaa gcggaaccgc caacaaaaac ggaacgccca caaaaggcac ctgaacagag
     4621 tgtgactgta cctatgaatg acgtaccgca ggcacaatca atcggttcag aagagacaaa
     4681 aaactaccac caagcaccaa gcgcaccgat acaagaatta gtcccttcca gcacacaaga
     4741 taacgaaggg actattcatt ttgaaaaaga cgcgtctgta gaaacgttta tccgtaaaat
     4801 cggtgaatct gctcgtaaaa ttggacaaga aaatgattta tacgcttctg ttatgatcgc
     4861 gcaagccatt ttagaatccg caagcggaca aagtcaattg gctcaggcac caaactacaa
     4921 tctatttgga atcaaaggca cacataacgg aaagggcgtc tcttttgcga cacaagaaga
     4981 tttaggcaat gggacacttt atacgaccca agcaactttt cggcaatatg agagctatga
     5041 agaaagctta aaagactatg ccaaactgtt aaaagaaggc ttagttggaa atacctcttt
     5101 ttatgatggt gtttggaaaa gtaaggcaaa aacctatcag gaagcgacta aatttttaac
     5161 aggaagatac gcaacagaca ctagctacga taaaaaatta aatgggttaa tcgaaaccta
     5221 tgacttaatc aaatatgata aagaagtagc tggtccacaa ctaagcaaag aaggctatat
     5281 ggtacctcta aaaaattatt caatttctag ttccttcggt tggcgaggag aagaatttca
     5341 tagaggatta gacatggcag ccaatcaagg agaaccaatc tatgcgagca aggcaggaac
     5401 ggtcatagaa gcttcgtatc atactttgtc aggaaattat gtcacaattg agcatgaaga
     5461 cggaagtgtg gctctttatg cgcaccaaca ggaatattgc gtgaacgtag gacaaacagt
     5521 tgaacaagga caaattattg gttatgtggg ttcaacagga aatagtacag gaccacattt
     5581 acatttagaa cttgccttag atggtagttt aacaaaagac aaattaatca acccagaaac
     5641 agtcttattc taaagaataa ggctgtttct gataaggagt ggtatagaaa atgtggttac
     5701 tttttaaata tttgatatat ttgtatctct cattttcgtt ttttaatttg aaatctcgta
     5761 tctatctaga acgtagttta
//
LOCUS       DARDHD010000148         5780 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000148 DARDHD010000000
VERSION     DARDHD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.150"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(89..1219)
                     /locus_tag="SDA06_002242"
     CDS             complement(89..1219)
                     /locus_tag="SDA06_002242"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG4229498.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1278..1505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1867..3069)
                     /locus_tag="SDA06_002243"
     CDS             complement(1867..3069)
                     /locus_tag="SDA06_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4229499.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="SDA06_002244"
     CDS             complement(3129..3332)
                     /locus_tag="SDA06_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG4229500.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="SDA06_002245"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="SDA06_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4869..5681
                     /locus_tag="SDA06_002246"
     CDS             4869..5681
                     /locus_tag="SDA06_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229501.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1709 a         1189 c          921 g         1961 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta
//
LOCUS       DARDHD010000149         5719 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000149 DARDHD010000000
VERSION     DARDHD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.151"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1910
                     /locus_tag="SDA06_002247"
     CDS             <1..1910
                     /locus_tag="SDA06_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG4229502.1"
                     /translation="IQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNS
                     NGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQF
                     KLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCL
                     QTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQD
                     SFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNV
                     TKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQG
                     GVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLE
                     NYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITK
                     IEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLIN
                     SYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIK
                     VNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEIL
                     LPSSLKSYFRL"
     gene            2092..2331
                     /locus_tag="SDA06_002248"
     CDS             2092..2331
                     /locus_tag="SDA06_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229503.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2347..3720
                     /locus_tag="SDA06_002249"
     CDS             2347..3720
                     /locus_tag="SDA06_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229504.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            3724..5358
                     /locus_tag="SDA06_002250"
     CDS             3724..5358
                     /locus_tag="SDA06_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229505.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            5521..>5719
                     /locus_tag="SDA06_002251"
     CDS             5521..>5719
                     /locus_tag="SDA06_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229506.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         1983 a          946 c         1173 g         1617 t
ORIGIN      
        1 ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact tataccataa
       61 aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca ttaagaaaac
      121 acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag attcgatttc
      181 attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa aaatatccgt
      241 tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa tattattttg
      301 accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa gaagaaaaag
      361 ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa gtcattacac
      421 ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa ggtggatgtt
      481 tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa gctagaagag
      541 aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc ttttttgaac
      601 atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta caagattctt
      661 ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta acaactaatg
      721 gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg aacttagaac
      781 ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt acgaaggcta
      841 atactttttt gagtttacta attaacctac agggcaagag cacaacgggt aaaaccacta
      901 cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga aatatgaatg
      961 ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt ccactaatac
     1021 tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat caattagctt
     1081 caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa gagacttttt
     1141 ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat gagaaattaa
     1201 ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag gatgcgaatc
     1261 acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata aagttatttc
     1321 ttttgaaact ctttgaacaa gggattacaa aaattgaagc aacttttgtc gaagctcaaa
     1381 aaaaactatg ggctgtttta cctgaacatt ctttgaagca acgcattggt aataatctag
     1441 ctttgattta tagtggagcc ctcctaattc aagatatact gggctatcct ttacgattag
     1501 agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat actagtttag
     1561 gaagtgagca aacggtattt gaccgagtga aaacattgtt ggtgatgaat agccataaat
     1621 ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa gtatcaattc
     1681 aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac ttgttaaggc
     1741 gagaatttca agtaaaggat ctaacctatc tttttaggca attgcttgat tcgggtaata
     1801 tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa gttcttccaa
     1861 catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa tttagaataa
     1921 accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat ttagcaaaac
     1981 aactgattac ttatctgtat gatagcgagc aaacccaaac ggaagtaaat gaagcttgat
     2041 ctatgcttca tttacttcat tcaatcaaac atgaaagaaa ggaaaattct aatgactaaa
     2101 aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt ttcaaatcaa
     2161 gcctttttta caaaaccaat ttacttggat ttcgaaataa gaggggtatc tgctggatct
     2221 atcgggggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca ctttgtgcaa
     2281 cgttcgttca atagaagagt cgtgaaaaaa tggtatttag ttcattcata gagaggggaa
     2341 tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc gaaaattagt
     2401 aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga acaaattccc
     2461 caacgatttt cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc ttttcgacag
     2521 cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtttg gcgttatgat
     2581 cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca acaacatcag
     2641 gttcaagtgt attcgatttc tgaacctctt cctgcttctt tctcacacgc gtcaacgaat
     2701 aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt cattattgag
     2761 aatgttcgtg ccggactttc ctataaaaag caacaacggc aatatttgtc ttctgcggta
     2821 ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagtacacga agcaaggatt
     2881 gttcgctata tttttcaact ttatttgacc aaaaaatatg gctacaaaaa gctttgtcaa
     2941 cgattgacgc aacaaaagct tttcttccgc gaacgtcctt ttcagcccta tcatatttat
     3001 agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt ggggaagtat
     3061 ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt tacaagccca agagattcgc
     3121 caatcaaggt gtacagctaa aaaagatacg tatctgtact tattacgtca aaaaattcgg
     3181 tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa gaagacgaag
     3241 acgcttcatt attatcattg taccgaccga agctgccaag gaatttttct tcgggcagaa
     3301 agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga aggcgtctat
     3361 caagccttga ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga tgaaaaacgc
     3421 aaagtaatcc aacaaaagaa acaagaagca caatggatta aggcatttga agagggcaat
     3481 tgttcactgg aagaactcaa gcaacaactg caagctcttc aaaagccgat gtctacttct
     3541 tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca actaaaagac
     3601 ctctctagtc gacaattatt atgtgactat gtttgtcgga ttgaggtaac aaaggataaa
     3661 acagtttgtg ggatttactt ccgacaactg acacaaaata ttttagaaag gaacgaatga
     3721 tctatggaaa tgatacaaac caaacgagtc gcattgtata ttcgcgtgag tacacaagaa
     3781 caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca gtttgccgaa
     3841 cggatgggct atgaagtggt agaagtttat gccgatgaag gaatcagtgg gaaatccaca
     3901 aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg ttttgatcta
     3961 ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt acaaacagtc
     4021 gaggaattat tgcgatatga cgtaggatta cattcgatct cggaacaatt tgacgtgacc
     4081 acttcgacag gtaaattgat gttacaactc cttgggagtt tcggtgaatt tgaacgcaac
     4141 caaattgcgg agaatgttca aatgtcaatg caaagcttag tgaaagataa aaagcgttat
     4201 gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca cgaatggtca aaaacaattg
     4261 gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata tttaggcgga
     4321 gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac agtcaaaaag
     4381 aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta tgcagggtat
     4441 ttagaatatg cgcgctacat caattgggaa accaaacgac ggaaggggaa gaatccgaga
     4501 ccgattttag tgaaaggaac ccatgaaccg attatttcgg aagaagatta tcaaaaagta
     4561 caagaacgcc ttgctttaga aggaaaacaa cccaaatgga accatacagg tgaaaatgta
     4621 ttgacaggct tgttacgttg tccagaatgt ggcgcaccga tggcagcgag taatgtcaca
     4681 aataccctaa aggatggcac aaagaaacgg attcgatatt attcttgttc agtctttcgg
     4741 aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc ggaaacattt
     4801 gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg attggtggca
     4861 gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact cgtggtgtta
     4921 ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc tttggaagat
     4981 agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga aaaacgacga
     5041 cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaaggaga gccgattcaa
     5101 gtcaaagatg tccaacggat attgactaat ttggatcgtt ttcttgccca gagtgagaag
     5161 aagcaaatca aagccttgta tcgtaccttt atagagaaaa taacctttga tcctaaccat
     5221 aaggagatgt tggaaattac gatgaaattc acgcatgccg tggtacaaca attgaatgag
     5281 caatatcaaa tagccgtatc caaaacaaag gatacggcta tttttgtgtt aaaaacaccc
     5341 tttacgttaa cgatctagtc taggttatat caaacaacgt tagaggggcg ctaacgcttc
     5401 tctagcgatt tgaaatcgtt agagaagtag ttagggaggt ccttatcata aatagggata
     5461 tttattaatg aatatttgaa atataattgt atataagaat taaaaagagt ggggatagat
     5521 atgaaaaaga tgggtaagtg tattttatgt gaaaaataca ctgaacttac taaagaacat
     5581 gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg taatttagat
     5641 gatttcctta agagcgattt tacaaaaggt gattttccga aggggattaa aaggaaacct
     5701 caaggaaatg tttattata
//
LOCUS       DARDHD010000150         5651 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000150 DARDHD010000000
VERSION     DARDHD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.152"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(103..1500)
                     /locus_tag="SDA06_002252"
     CDS             complement(103..1500)
                     /locus_tag="SDA06_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4229507.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(1532..2503)
                     /locus_tag="SDA06_002253"
     CDS             complement(1532..2503)
                     /locus_tag="SDA06_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229508.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2877..3302
                     /locus_tag="SDA06_002254"
     CDS             2877..3302
                     /locus_tag="SDA06_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229509.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(3790..4491)
                     /locus_tag="SDA06_002255"
     CDS             complement(3790..4491)
                     /locus_tag="SDA06_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4229510.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(4507..4947)
                     /locus_tag="SDA06_002256"
     CDS             complement(4507..4947)
                     /locus_tag="SDA06_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4229511.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(4947..5522)
                     /locus_tag="SDA06_002257"
     CDS             complement(4947..5522)
                     /locus_tag="SDA06_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG4229512.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1627 a         1176 c          901 g         1947 t
ORIGIN      
        1 agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt
       61 aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt
      121 tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg
      181 tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt
      241 atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg
      301 gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc
      361 ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc
      421 gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat
      481 ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc
      541 ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg
      601 ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc
      661 tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa
      721 ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc
      781 tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat
      841 gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc
      901 caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg
      961 ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt
     1021 ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac
     1081 cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata
     1141 cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa
     1201 acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat
     1261 agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt
     1321 cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt
     1381 tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt
     1441 ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat
     1501 caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca
     1561 tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg
     1621 ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca
     1681 gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac
     1741 cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag
     1801 cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt
     1861 ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg
     1921 ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat
     1981 gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata
     2041 ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga atactcattt
     2101 ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga
     2161 tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc
     2221 cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct gagacaactt
     2281 ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat
     2341 tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta
     2401 tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca
     2461 ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc
     2521 agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt
     2581 gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta
     2641 ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa
     2701 gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat
     2761 tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt
     2821 gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga agtattttga
     2881 agaaattaat cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt
     2941 tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg
     3001 aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat
     3061 ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac
     3121 aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca
     3181 ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca
     3241 tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat
     3301 agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc
     3361 aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat
     3421 aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac
     3481 tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat
     3541 gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga
     3601 acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat
     3661 ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa
     3721 ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt
     3781 tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc
     3841 agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca ccttttttgc
     3901 atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac
     3961 atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa
     4021 cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat
     4081 cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc
     4141 atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt
     4201 tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc
     4261 tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa
     4321 gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc
     4381 gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt
     4441 caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt
     4501 cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc
     4561 acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt
     4621 aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa
     4681 ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa
     4741 ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct
     4801 gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt
     4861 gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa
     4921 tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta
     4981 atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga
     5041 tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag
     5101 ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt
     5161 gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg
     5221 aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga
     5281 ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata
     5341 cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag
     5401 tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc
     5461 gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc
     5521 ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat
     5581 aaaagactag attttgtagt tttttagtgg tatataggat agacgccact atatatagta
     5641 tttagaaaaa a
//
LOCUS       DARDHD010000151         5597 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000151 DARDHD010000000
VERSION     DARDHD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.154"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            81..1061
                     /locus_tag="SDA06_002258"
     CDS             81..1061
                     /locus_tag="SDA06_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229513.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1183..2601
                     /locus_tag="SDA06_002259"
     CDS             1183..2601
                     /locus_tag="SDA06_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG4229514.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2672..4141
                     /locus_tag="SDA06_002260"
     CDS             2672..4141
                     /locus_tag="SDA06_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4229515.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4151..4618
                     /locus_tag="SDA06_002261"
     CDS             4151..4618
                     /locus_tag="SDA06_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4229516.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4654..5526
                     /locus_tag="SDA06_002262"
     CDS             4654..5526
                     /locus_tag="SDA06_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4229517.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         1886 a          794 c         1176 g         1741 t
ORIGIN      
        1 gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc gagtaggtag attttctatc
       61 tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag agaagttgcg aaagaggcaa
      121 atgtttctcc tacgactgtg tcgagggtat tgaatgatcg tggatatttg agtgaggaaa
      181 caaaaagaaa agtttttgat gccatgaaaa aattagaata ctatcctaat gaactggcga
      241 gagcactttt taaaaacgaa acatataccg taggtttgat ttttcctaca attactaatc
      301 catttcacgc tgagttaatt caagatatcg agttcgagct ttccagcagg ggatataaag
      361 tgttgctgtg caatagctta aacaatccaa caaaagaaaa agagtacttg acgatgttga
      421 gaaagaatca ggttgatgga attatagtag gaacgcacaa taaagatatt gcggattatg
      481 atattccagg gctacctatt gtagcgattg atagaagtct tggtgaaaat acagttacag
      541 tatcttgtga taattacagg ggtggggaat tagctactca actacttgta aatcatggct
      601 gtcagaatat actgtgtatt cggggagata gtaagataaa gctacccgcg aatagtagga
      661 ctttagcata taaagatgtt ttgaataaat tcatgctgga accgttagtc ctagaagtac
      721 catttgtaaa agaaatcaat gaaaaaaatg aaataattaa gacatatctg gaaactcatc
      781 caaatattga cggggttttt gcaggagatg atttattggc ttctttggcg attaacaact
      841 taggaagttc gggcaaaaga gttccaaaag aggttaaagt gattggattt gatggagctc
      901 aacaaacatt gatttataat ccagaattat caactattca acagcctata gatcaaatat
      961 ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg cgaaaaggag acggatgaac
     1021 tttcacttcc agtaaagctc ataaaaaaaa atacagcata agttaaatcg gcgaaaaatt
     1081 ccttcgtcga tttaaaaata tttttatgaa acgggttgac atatgaaacg ggttgacata
     1141 tattagttgt gaagataaaa accttttcac tggaggaaga aaatggagaa attattaatt
     1201 aaaagcaata atcatcaact ttactaccaa cctaaggatg tttgggtcgg agatgttatg
     1261 ccttacggga aagaaggaaa attctttatt tatcatcaac gtgatactag aaatccagtt
     1321 ccttttggag aaccgtttgg ctgggcttta gcaactacga aagattttgt tcattttgaa
     1381 gactttgggg aatctctttt aagaggggat gacaatgaga gagaccagtt tatctacgct
     1441 ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt atacagggta taatcgtgaa
     1501 ttcttagcgc agggaaagac atctcaagtg ttgcttcatg ctgagagtga tgattttgtt
     1561 cattgggaaa aatcagaaac agcattggag ttagaaccac aagaagggta tgatattcga
     1621 aactggagag atccatttgt cgtttgggat gatgaaaaag aagaatattt gctaatttta
     1681 ggggcgcgaa agggtcagga taaacatcta caaaccggac gtttagtgga atttacatca
     1741 aaagatttaa ctaattggga attcaaaggt gatttttggg ctccgggatt gtataccatg
     1801 tttgaaatgc cagaactttt caagattggt gactggtggt atcttgtcta ttcagaatac
     1861 tcagatggaa ataaaatact ctatcgaatg agtcaaagtt tggaaggtcc ttggattaag
     1921 cctaaagatg aagcttttga tggacgggct tattatgcag gtagaacagc ttatgatgga
     1981 aaacgtcgag tcctatttgg atgggtacca acaaaagatc aagaaaatga taaaaacaat
     2041 tatttatggg gaggtacttt ggttcctcat gaagtctatc aaaaagagga tttttcactt
     2101 ggggtaaaac ccatagacga agtgtggaat gcattcataa atttacatga tattgccgat
     2161 gtagagatag aaacagcaga cacaagagaa gaagtaacgc ttgtcaaaaa tagtggaact
     2221 ttgtttaatt tagaaacaac aatttcattt gatgagaatc ttagtgactt ttcactgaga
     2281 ttatataaaa accttgagac agatgaatct tacgaatatc gcttcaattt agatgagggg
     2341 cgtcttacat ttgataaaaa cccttgttac ccttggtttc aatatatgga taaaggactt
     2401 attagaccta tatctttaaa gcctaaccaa tcttataaac taaaagtgat tgttgatgac
     2461 aatataatga cactttatat tgatggaatc gctcttaacg ctcgtgtaca tggttgtttt
     2521 ggtcaagcaa ttactattac agcaacaaat ggatcaatga ctgcaataaa taccaagttt
     2581 tcaaatgaaa taaagaaata ataaacgtca atcatgtggc aggtctgaaa atcaaacatg
     2641 acctgtcaca tttaaaaggg aggataaatg aatggactac aaaaaaattg ccgaagatgt
     2701 attggaaaac gttggaggaa aagagaatat ctcaagcgct acacactgca ttactcgttt
     2761 acgtttaatt cttaaagaca aaagcaaagc gaatatggaa atgcttgagg aaatagaagg
     2821 tgcaaaaggt gttcttttca atagtggaca gttacaagta attttcggaa caggtataat
     2881 tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct aaggaagtat ccgtttctga
     2941 aatgaaagac gaaggagtca ataattcagg aaatagactt caacgagctt ttaaagtttt
     3001 ctcggatatc tttattccga tcattccagc ttttattggg gcagcaatgg ttttaggaat
     3061 tcgttcgttg attaccactc ctggaatgtt tggaatggat ggctcattcg ctgatcacaa
     3121 tgtattccta aatgatttat ccaaatttct gggtattatt gcaaccacat ttgattattt
     3181 accagtttta gttatgtatt ctgcaacaaa acgttttggc ggaaacccta ttttaggatt
     3241 acttgtagga tttgttatga ttcatccagg actagccaat cggaatgatt tcgtcatggg
     3301 aactataaag cctgattatt ggcatttatt tggattaagc attccagcag ttgcgtttca
     3361 agggggtgtt tttccagcaa ttttgacttc ttggttttta gcaaaagttg agaaaattac
     3421 aaataaatat tctcctcaag tacttgctta cattttagtc ccaactgtga caattgttct
     3481 tgccaacttt gcactatttt tagtgtttgg tccattgggg aattttatcg gtaatgtttt
     3541 aggtggaatc attgatttcc tatatatgaa gatgggtgcc tttggtgctt ttgtttttgc
     3601 aggcgcgtta caaccattag ttgtaacagg aacacaacat gctattcaag gtattgaggc
     3661 aaacttagtc gcaacaactg gattcaatta tatccaacct atttggtcag tttcaattat
     3721 tgcccaaggt ggcggttgta ttggtatgta tttactcttt aagaaaaaat ctaaagatag
     3781 agatattgcg atttcaagct ttattccaac actggtaggt attactgaac cggctatctt
     3841 tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc tcgtttattg gtgctggttt
     3901 tggtggagca tttatgaaaa tttttgatgt aaaagccatt ggacaaggat tgactgtatt
     3961 gccaggtctg actattgcta atccagttct tccttatata tttggtaatc tcatagcatt
     4021 cattttacca atcattttta ttctcgctta tgataaatat aaagtgttta tccctgaagc
     4081 taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa ttgccagttc aagtaaagtg
     4141 agatgagagc atgttaaatt tttttagaaa aaatgaaata agcgttgtag cacctgcaac
     4201 cggtgttttt gaaaaactgg agaaattaag tgatccagtc ttttcaaaag gaatgatggg
     4261 acaaggattt gcaattgatg caaaaaacga aacgattgta tctccaatcg atggttttgt
     4321 ttcaagtatt tttcctacaa aacatgcctt aggaataaaa acaaaatcag gaattgagat
     4381 acttgttcat gtaggaatag atacagttga gctaaatggt gagggatttg atattaaagt
     4441 tcaagaagga caaaaagtta aggctgggga tattcttatg accattaatt ttgtagtttt
     4501 tgaaaagaat cacaagacta aagatgtcat tgttgttttt ccagactacc aagacgaaat
     4561 tttcattccg aaacttgaag aagtaaaaca aggacaagaa ttgtttgttc ttggctaatg
     4621 ataagtaatt aataatagaa tcgaggaaaa tgtatggttt ttggggctat tgaagctggt
     4681 ggaacaaaat ttgtttgtgc cattggagat aaagatttga acatatttga aaaagtcagc
     4741 tttcccacga ctacacccaa agaaacaatg aatttagttt tagatttttt taatcatcat
     4801 aaagatgatt tagaaggtat cggagtggga tcgttcggtc ctattgatat tcattgtgaa
     4861 tctaaaacgt atggcttcat tacatcaacg ccaaaacttg cttggcagga ttttgatttt
     4921 attggaagtt taaaaaagca acttaatatt ccaactgctt ggacgactga tgtaaatgcc
     4981 tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc tttcaagtgt tgtttactat
     5041 accgtaggaa cgggcatagg aggtggcgct atccaaaacg gaaacttcat tgaaggtttt
     5101 agtcatccag aaatgggaca tatgcttgta aaacgacaca ataaagacaa cttttctggg
     5161 acatgtccat tccacggaga ctgtttagag ggcttagctg ctggtccggc aatcgaaaga
     5221 agatcaggga aaaagggaca agaagtgact gaaaatgacg ttacttggga aatagaagct
     5281 tattacattg ctcagtgtgc atataataca acattgatgt tatcacctga tgtcattata
     5341 tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga aagtacaaga taagtttatt
     5401 gagttgatca acggatatgt cgctattccc gatgttgaca attatattat gacaccaaaa
     5461 ttgttagata attctggtat tgtaggttgt ttagctttag caagagacgt taaagaaaaa
     5521 tcttaatttg gaaaaaagat cgtctcgact atttgtttgg ttgatgcgat ctttttttca
     5581 attagtaatt gaaggga
//
LOCUS       DARDHD010000152         5586 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000152 DARDHD010000000
VERSION     DARDHD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.155"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            58..285
                     /locus_tag="SDA06_002263"
     CDS             58..285
                     /locus_tag="SDA06_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229518.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            565..2388
                     /locus_tag="SDA06_002264"
     CDS             565..2388
                     /locus_tag="SDA06_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4229519.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            2402..3811
                     /locus_tag="SDA06_002265"
     CDS             2402..3811
                     /locus_tag="SDA06_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4229520.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            3829..4665
                     /locus_tag="SDA06_002266"
     CDS             3829..4665
                     /locus_tag="SDA06_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229521.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            4730..5512
                     /locus_tag="SDA06_002267"
     CDS             4730..5512
                     /locus_tag="SDA06_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229522.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT         1745 a          929 c         1219 g         1693 t
ORIGIN      
        1 agttaatttt taagaatact agcccaaatc attctatcga tacctttata tctcggaatg
       61 attgaaattg gctattgctt actgattttt accaaaaaaa caagtatcac tattgttgcc
      121 tatattgttg gaatgctgtt tattccatcc tttactcacc aaattcatca atttttccca
      181 agagcagaat tgttgaagtt gtgtgatccg ttatatgcat ttttgatgct ttcacgattt
      241 tgggaatttc ccgctggtta tgtaggaatg gttttactgg tatagttaat attagatata
      301 cttattttag cagtaagtat acgcagatat accctagttg atgtgatcta actgttatat
      361 taccctatac ggtcaaacga aaagataaaa aattaaattc cctgttgatt gcgctatcat
      421 aatgtgctat tattggatta cagcaaaata acgttattgg catgttactg attatgcagg
      481 caagaccaag acaaactaac acttcaatgt tgattggggc gtggtttgac ttggtctttt
      541 ttactgcttt aaggagagag aataatggat tacagagaag taggaagaaa tgtattgcaa
      601 caggttgggg gaaaagaaaa tgtggtcagc ttaacccatt gtgccaccag actgcgtttt
      661 gaactcaaag ataaaaggaa agccgatacg aaagccttag aaaaaacact tggtgtaatt
      721 agtgtggtgg atagcggtgg acaataccaa gtggtgattg gcaatgaagt ccaaacagcc
      781 ttcaaggaaa ttcaaaagga agtgggaact tcaattaaaa agggcccctc ttcagacaat
      841 caagaaagta ttatttcaag attgattagt gtcatttcta ccacctttac accgatgatt
      901 ccagctataa caggtgccgg gatggtaaaa gccattttgg ccgtcttgac tttgactggt
      961 gtactatcag acagtagtca aacttacatc attttgaata ctattgctga tgcagccttt
     1021 tattttatgc cagtactatt agcctatggg gcagccataa agtttgatgt caacccaatt
     1081 ttggcgatta ctgttgctgg cattttgctt catccaaata tttcacagct tttttctact
     1141 ggcgatgccg tttcttttct aggaattccg gtattagctg cagattatgc gggatcagtt
     1201 ttaccgatta tctttactgt ttggatcatg tcctatgtag agccctttgc ggatcgaatt
     1261 tcgccttcgt ttattaaatt ctttactaag ccgatgctgg tatttttaat tatggggcct
     1321 ttagcgttgg ttgtcattgg accgtttgga acgttgctga acgatcttat agcaatgggg
     1381 gctgaatgga ttaacggcaa agcagcttgg ttaattccat ttttgatggg tggtttgcag
     1441 ccatttttgg tggttacggg tacagcttgg gcaatgacgc caatcgctac tatgcaattg
     1501 agcagtacgg gttctgaaat gatcaatggg ccaggaatgc tggcttctaa tattgctcaa
     1561 tcaggtgcta cctttgctgt agccgcgaaa actaaaaaca aagagttgaa gcagctagct
     1621 gtgtccactg ggatcactgc tttaatggga attactgagc cttcactcta tggagtaacc
     1681 ttgaagctga aaaaaccatt agttgcttcc atgattggtg gtgcaattgc tggtttgtat
     1741 gctggcttga gcggtttagt cagatatgcc tttgtttccc ctggcttagc agccttacca
     1801 gcctttattg gagaaaatcc aatgaatatt gttcacgcaa ttattacgtg tgtaatcgcc
     1861 tttgttgcaa cctttatcat tgcttatcta attggatttg acgatacttc tgatgaatca
     1921 gagcaaacgg caatagccga agcagtcatt gttgaaaatc cggtaaaagg aaccgtgtgt
     1981 cctatttctg aagtgaacga tgctgtcttt tctaagggac tattgggaaa gggcgtagcc
     2041 attcatccag aagaaaacag catttattca cctgttgagg gagaagtcat tactatttta
     2101 gaatctaaac atgcgattgg tctacgttct aaagaaggta ttgaattatt gatccatgtg
     2161 gggatcgaca ccgtgaattt aaaaggggaa ccctttgaag tatttgtaaa agcaggagat
     2221 caggtgtcag caggacaatt attaatcaac gccgatttcg ctgccattag aaacgctggc
     2281 tacgatgata cagtgattgt ggtagtgacc aacagtgaaa attacgcagg aattagtgaa
     2341 gcaagcggaa gcttaaagga acacgaacca ttgatgcaaa ttagttaagg agaggatttt
     2401 tgtgcaatac aaacagcttt cagcatttcc agagaagttt ttatggggtg cctccattag
     2461 tgcctaccag gcagagggtg cctatcagga ggatgggaaa caaccgtcta ttattgacaa
     2521 gtatcagcat acagaagggg tagctaattt tgaagttgcc tcggattttt accatcacta
     2581 taaagaagat atacagctgt ttaaagaatt aggcttaaaa gcttttcgct tttccatcgc
     2641 ttggacgcga attatggata ttgaaaataa taaaaccaat caaaagggaa tttcttttta
     2701 tcatcaagta attgatgaat gcctgaatca gggaatcgaa ccgattgtca ccatgtacca
     2761 tttcgattta ccttattttc tagaggagca gggcggttgg ttaaatcgag caactatcga
     2821 ttattttgtg gactatgtac aggtattgct tactgagtat ggtgacaagg tgaactattg
     2881 gctgacaatt aatgagcaaa acacgatgat ccttcatcct ggagcgattg gtttgccacc
     2941 aagtggagag ctgccttcaa aaaaagaact ctttcaaatt aatcaccatg ttttattagc
     3001 tcaagctaga gtaattcact tgtatcatca gttgaatctt aaaggaaaaa tcggtccagc
     3061 aatcaattta acggctatgt atcaagctac ctccagtcca gaagatgcaa ttgcagccca
     3121 taattgggaa acattgagag gctggtcttt cttagatgta gcagttcggg gaaaatacaa
     3181 ttatttgttt gaaaactatt tgaatgatcg tggattatat cctaaaattg aggcagagga
     3241 tcaggcaatt ttatcctccg gtaaacccga ttttattgcg attaattatt attcaactgc
     3301 caccatcgct gccagcaaaa atgacggatc agatgtttcc gctagagcgg gggatcaaca
     3361 gatcatgttg ggagaagaag gtgtctaccg ggcagctgaa aatccttatg tagataaaac
     3421 gccatatggc tgggtagttg accctactgg actacgatta acattgagga aattgtacga
     3481 tcgctatgat ttacctattt taattactga aaatggctat ggggcacccg acatagttga
     3541 ggaagataga cgaattaatg accaggatcg aattgattat ctagccaaac atattctggc
     3601 tattcaagca gctttaacag atggtgtaga tgtttttggt tatttacctt ggtcagcgat
     3661 tgatgttgtt agtacccatc aaggcttcaa taagcgttat ggttttatat atgttgaccg
     3721 aaccgatgag aatcttaaag aattaaaacg aataaaaaaa gaaagcttct tttggtatca
     3781 aaaagtaatc gcaagaaatg gcttagctta gagagaacgg tgaatctaat gagagtaatt
     3841 aaaacgttaa acaacagttt aattctttgc ttagataagc aaggcaaaga agtaattttg
     3901 atggggaaag ggattggatt tcaactaaag aaaggcgatt tggttccggc tgacaaaatc
     3961 caaaaagtat ttaaattaga ttcactttct gatgctctaa aaaaggatta cattaaactg
     4021 tttgaggctg ctccggatga tctaattatt tgcgtcaagc gaattatcga ttatgccaac
     4081 gccaagtttg actttaagct gaataaaagt ctatttttta ccttggttga tcacttgaag
     4141 catgccattg aacgcagtca aaaaggcatt gtttttcaaa atcgcctgct gattgaaatt
     4201 caaaagtatt acccaaaaga atttgagatc ggctgctatg ctatccaatt gatcaacaaa
     4261 aaaatgaatg ttcagttgcc agaagaggag gctggaaaca ttgcgtttca cattgtaaat
     4321 gcccaaagtg aacaagcaaa tatggaagaa accatgctct cggtaagagc actaaaggat
     4381 atcttggcta tgctaggata tctgtttccc gaaaaaaagg ttgataaaga ttcgatgctt
     4441 tacttaagat ttgtgacgca tttacagttt tttattgccc gaatgattaa gaaggaagag
     4501 ttgccggaga aggatgattt tttgctggct tcagtgaagc agcgttttcc gaaagagtat
     4561 cgggcggcct tgaaaatcaa ggagtacgtt caagaagaga tgggtgcctc agcaaatcag
     4621 gacgaactgc tttatctaac actgcatatt tcaagggtgt actgaatgaa gaaaatatcg
     4681 gctgcacaat atataaagac ttgttggttg tgctaaggct cctgattcct tggaaaattc
     4741 ttctaacagt gcggaggtga atgtttcgac ctctaccagt gaagtggtaa agtgttcaca
     4801 agaaactact agcttgccag aagagctact tggagattgg cagattaata ataaggatgc
     4861 tggtatagag atatcagaca gtaaagctgt attccgtata ggaaggtctc aagtagagtc
     4921 aaaggattat tatgtagatg taaacaacaa aatgctttta catgtctatt tttcttttga
     4981 gtatgatagt gtgaaataca ttgaagtctc tattgaaaga actggttata gtgaatttat
     5041 tagtaaaagc gtgggaattt taactatgca cactaaagat ggacaatata taagtttggg
     5101 tgtgaagaga gtttccggtg agaataatga gacagaagca gttacggagc agacgatgga
     5161 tgaaagaatc gattcatcta agagtaatga gacagaagta gatactggga agacgataga
     5221 tgaaagaatt gattcattca tcgaaacata cacagctata ccacctactt ggaagcttgt
     5281 aaatgaagat aaaggaatca cgataaatac tgatacagag atttctacag atgctcaatc
     5341 tttatcagat ttattagcta atctaacagg tatcagtaat gcagtgacag agcaagttgg
     5401 taaagacatt ccacttcggt tacttgaaaa tgggaaaaaa gattattctg tggtatttaa
     5461 gaacggacag gtaactgatt acaacgaagt atttaaaaaa gttattgaat aatgattctt
     5521 ccatcttttt catttattta ccctaccccg ttgttatatg accgtactta tacatctcca
     5581 gtagct
//
LOCUS       DARDHD010000153         5540 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000153 DARDHD010000000
VERSION     DARDHD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.156"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(286..789)
                     /locus_tag="SDA06_002268"
     CDS             complement(286..789)
                     /locus_tag="SDA06_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4229523.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1148..2173
                     /locus_tag="SDA06_002269"
     CDS             1148..2173
                     /locus_tag="SDA06_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4229524.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2496..3374
                     /locus_tag="SDA06_002270"
     CDS             2496..3374
                     /locus_tag="SDA06_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG4229525.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3850..3966
                     /locus_tag="SDA06_002271"
     CDS             3850..3966
                     /locus_tag="SDA06_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4229526.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4894..5232
                     /locus_tag="SDA06_002272"
     CDS             4894..5232
                     /locus_tag="SDA06_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229527.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5326..>5540
                     /locus_tag="SDA06_002273"
     CDS             5326..>5540
                     /locus_tag="SDA06_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229528.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYV"
BASE COUNT         1870 a          838 c         1029 g         1803 t
ORIGIN      
        1 aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat
       61 tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa
      121 ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt
      181 agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga
      241 tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac
      301 agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat
      361 aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca
      421 taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat
      481 accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt
      541 tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac
      601 aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa
      661 agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag
      721 cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac
      781 ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca
      841 taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt
      901 ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct
      961 atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg
     1021 tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc
     1081 tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag
     1141 ggatagattg ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat
     1201 catgaatctg atgctactta tctttattgt attatggtta ggttacataa atcatgattt
     1261 tttgatttca agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc
     1321 gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt
     1381 tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc
     1441 tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt
     1501 tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt
     1561 ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt
     1621 actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga
     1681 agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta
     1741 tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat
     1801 tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc
     1861 gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac
     1921 catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc
     1981 agttgctaat atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc
     2041 atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg
     2101 tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca
     2161 aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca
     2221 caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa
     2281 aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta
     2341 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
     2401 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
     2461 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
     2521 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
     2581 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
     2641 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
     2701 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
     2761 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
     2821 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
     2881 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
     2941 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
     3001 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
     3061 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
     3121 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
     3181 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
     3241 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
     3301 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
     3361 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
     3421 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
     3481 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     3541 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
     3601 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
     3661 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
     3721 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
     3781 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
     3841 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
     3901 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
     3961 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
     4021 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
     4081 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
     4141 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
     4201 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
     4261 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
     4321 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
     4381 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
     4441 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
     4501 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
     4561 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
     4621 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
     4681 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
     4741 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
     4801 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
     4861 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
     4921 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
     4981 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
     5041 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
     5101 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
     5161 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
     5221 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
     5281 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
     5341 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
     5401 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
     5461 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
     5521 gaagagtttt tggattatgt
//
LOCUS       DARDHD010000154         5534 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000154 DARDHD010000000
VERSION     DARDHD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.157"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..988)
                     /locus_tag="SDA06_002274"
     CDS             complement(<1..988)
                     /locus_tag="SDA06_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG4229529.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="SDA06_002275"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="SDA06_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4229530.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="SDA06_002276"
     CDS             1813..2967
                     /locus_tag="SDA06_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4229531.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="SDA06_002277"
     CDS             3182..4150
                     /locus_tag="SDA06_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4229532.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="SDA06_002278"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="SDA06_002278"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4229533.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="SDA06_002279"
     CDS             5180..>5534
                     /locus_tag="SDA06_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG4229534.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DARDHD010000155         5489 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000155 DARDHD010000000
VERSION     DARDHD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.158"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(268..1203)
                     /gene="whiA"
                     /locus_tag="SDA06_002280"
     CDS             complement(268..1203)
                     /gene="whiA"
                     /locus_tag="SDA06_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG4229535.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1237..2235)
                     /locus_tag="SDA06_002281"
     CDS             complement(1237..2235)
                     /locus_tag="SDA06_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG4229536.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2232..3116)
                     /gene="rapZ"
                     /locus_tag="SDA06_002282"
     CDS             complement(2232..3116)
                     /gene="rapZ"
                     /locus_tag="SDA06_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG4229537.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3251..3982)
                     /locus_tag="SDA06_002283"
     CDS             complement(3251..3982)
                     /locus_tag="SDA06_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG4229538.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(3986..5251)
                     /locus_tag="SDA06_002284"
     CDS             complement(3986..5251)
                     /locus_tag="SDA06_002284"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG4229539.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         1570 a         1173 c          905 g         1841 t
ORIGIN      
        1 attaatctcc agttatagat aaatacttga tcctatctat atcaggactt ctatattaag
       61 caaaagttta tgtataaaac taaaatcttc attcagttgt ctccccgctt tttatacgat
      121 tgtattaaaa aacaatagaa gcctttcaac attatgttac tccagtaaag ttggaattat
      181 agtttgacgg aagaaaagca agtatagctt ttaaaaaaaa ctgatacatt cgcatctcct
      241 gcttcatgta tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa
      301 ttcatttatt ttgcgaattc ggtgattaat tcccgatttt gatattgcac ctgagggaat
      361 catttcccct aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc
      421 ctgaagtttt tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc
      481 gatttgttta gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag
      541 acgattgacc gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga
      601 gttggttgca ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaatacga
      661 aatatagcca ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc
      721 gcaaatgtct tggttatgct gttcataaat agaaaatatt tccaaatgat aacgactcgt
      781 ttccggatta ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt
      841 ttgcgaattt cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat
      901 atcaagatcc atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt
      961 cttttttaat ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt
     1021 caaaagagaa tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa
     1081 ctgctggttc acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt
     1141 cgcatgttct cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga
     1201 catcctattc tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa
     1261 acggaacaat tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc
     1321 taaaaagtcg gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc
     1381 gacttgtact agatactcat catagatctc tgggtccata taatcttcag gcactttttc
     1441 cgtattgacc aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat
     1501 atgatcggca tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata
     1561 gacggtttca gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg
     1621 aaggatactg gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc
     1681 tgaaacgact tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac
     1741 acggtcaatg gttttacggt tgatcgcgat tttagattct cctactgcag tcgttccgtc
     1801 tttaaaaaca gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac
     1861 atgcatcatt tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac
     1921 tgcggcaatg atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa
     1981 acggtattgg aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt
     2041 tctcaagtca ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccacc
     2101 atcatctgca acagtcacta ctgctgtgat atctgctcct tgatttcgta aacttttcag
     2161 gataactggc agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta
     2221 ggttttcatt ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc
     2281 acattgactt tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa
     2341 cggtgttgac cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg
     2401 tatccaggca atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca
     2461 tcaaaggaca tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc
     2521 gggatataat gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc
     2581 aacccgtatt taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat
     2641 ttgaatgact cattgatttt ttcacgtaat tgtcgtggtg tcaatgttgt cgtatcaatg
     2701 atcacggaag cttgggcttt caagtcgtct agtatcgccc gttctttacg tatcccttca
     2761 gtgatcaaac cgtccatcgc cattggatgt gctcgtcttg tttctttgta gcgagccact
     2821 agttcttcgt ctgaagcatc taaaaataag atagaagtgt cgatgaagtt cgtattttcg
     2881 atttcgatca gcatgttttg gatttcttca aagaaggaac gagaacgcaa atccactcct
     2941 aaagccattt ttgtgacttt acctgattct ttgatcaatt cccagaattt tggaatcaat
     3001 cgaggcggca tattatcgat acaaaaatag cccatatctt caaaactttg gatagctact
     3061 gttttccctg caccactcat acctgttatg atgactaatt gcaaattatc tgtcatattt
     3121 acgaacccct ctcacattgt gcataataaa attatatcat cccgggcgta tcattaccag
     3181 ttttcttttt cttttacaaa aagatttgga ccaaatatct tttaggatat ttgccccaaa
     3241 tccaattttt ttacttttcc gatgctgtat ccaatgcctc gcttctttca gctaatgcac
     3301 gttcgatcgt tcgatccgct aaaatagatt gagcatcgat cactgggtaa tgattatctt
     3361 cagcaaattc atgcatcaaa gataattctg tgcaacctag gatgactttt tcacagttta
     3421 ggcgctctac cgcttcctct aagatttcgt aatagagctc ttgattcaaa taatccgatt
     3481 ccttgatctc atgatagatc aggtaattga ttttttcttg aagcgcagta tcaggaatca
     3541 ctgtttcgaa accaagattt ttgacttcgc gttcatagat tccagctttc atacttcctt
     3601 ctgttcctag aatcgctact ctccctgtcg tatgctgacg gacaagttca tttgctgctt
     3661 ctcttggcat atgcaagata ggaatatccg ttgccgcttg taactcttca aaaaagtagt
     3721 gtgcggtgtt acaagtcaac acaatgaagt ttggtcgcaa taaattttgt ttctcaatat
     3781 catccagcag aaacggcatt ggattttctt ctgatcgatc taaaatataa gctgtacgat
     3841 caggaactgt tgcatgattg aataagacat aattcaaata ttcttgatct tttgtagctt
     3901 tcgttcggtg gttgatcaag cgaacaaaac tttctgtcgc catcgtgccc attccgccta
     3961 aaatactgaa aaaattctcc atgaactagc cttctttcac atgaaaatac tttttgaatc
     4021 ttggaatata gttatggaac atatatttca tcaacaacca gcgcttgatt gatcggtctt
     4081 tttcataaaa tacagtagta ccgtaacgat tttctttgat catttgctca gctagttttt
     4141 tgtcttcatt ttcccgagca tattctaaaa agattttttt gggcacaccc atccataatc
     4201 ttgctttatc tgattgattc gttccatagg tcgtttcaac aaagggtttg ttgaacactc
     4261 gatcttctgt gacaaaacga gccagattca agccgttcaa tgtcacaaag aaactgctac
     4321 gaccttgtct caaattgatt tcaaataatt tatattctcc atctctagga tcatacttca
     4381 tatcgaaatt ggcaaagcct gtatattcga ttttttccaa aaatgcctta atggtttgat
     4441 agattttttc gttataatct ggcatgatca caacgtaatt accaatcgat gctggtgtag
     4501 gatcttctaa tagtggatga ccaaggcaca tcatccgaac ttgatgatct tcatctacat
     4561 acgcatttag cacacgcata ttactatcat cacccgggat aaaatcttgt acaatcattt
     4621 cacttttata accggcttcg tatatccggc ctaaaattaa atcgaactct tctctagtct
     4681 ctaaaataaa agcttttttt cttccttcaa attggacaga aagatactcc acactgtttg
     4741 caggtttcaa agcaactggg aattcaaaag gcaattcctg ttccaaatgt ccatttacta
     4801 acatttcttc tctgacgatc aatgttttag gatacggaag atcatattct tcacaaactt
     4861 cataaaagct tactttattg atcagccttt caaataaaga ataatcaata tatggacaga
     4921 taaaatattc tgacagctct tgtttatgtt gggagatcaa ttctgcatat ccatcaccac
     4981 aagcaatcaa aagatatttt ttactcttat cagaatatct ttcttttgcc aaacgaagca
     5041 tcgtctcgat aaatacaggg tctttatcaa atcctggaat aacttctaca ttgacaattt
     5101 tactgtagcg ggtaggggct aactgatccg aagcatatgc ttggcagatt ttgccgtatg
     5161 cttcattaaa agaacgtgcc ataccataaa cgttcatgtc gcttcctaat aatataggga
     5221 taaattcttc attttcaata ctgttcatca ttatctctca cttactcata atctcttgtt
     5281 tttcttttcc aactacacaa ggaatatcat accataaaga aaaaagcaat caaaggaaag
     5341 tcctccgatt gctcaattct tttttgaacg gattgaactt ataggaagtt tttatctata
     5401 ccaatcagtg tagaaagaac taccatgtaa tgattctaga acactgatat taataaaaca
     5461 agcaaaaatt cattttttta tgttttttt
//
LOCUS       DARDHD010000156         5449 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000156 DARDHD010000000
VERSION     DARDHD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.159"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..276)
                     /locus_tag="SDA06_002285"
     CDS             complement(55..276)
                     /locus_tag="SDA06_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229540.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(273..563)
                     /locus_tag="SDA06_002286"
     CDS             complement(273..563)
                     /locus_tag="SDA06_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229541.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(560..1744)
                     /locus_tag="SDA06_002287"
     CDS             complement(560..1744)
                     /locus_tag="SDA06_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4229542.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1926..3329)
                     /locus_tag="SDA06_002288"
     CDS             complement(1926..3329)
                     /locus_tag="SDA06_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4229543.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3351..4124)
                     /locus_tag="SDA06_002289"
     CDS             complement(3351..4124)
                     /locus_tag="SDA06_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229544.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4134..4511)
                     /locus_tag="SDA06_002290"
     CDS             complement(4134..4511)
                     /locus_tag="SDA06_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4229545.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4532..4846)
                     /locus_tag="SDA06_002291"
     CDS             complement(4532..4846)
                     /locus_tag="SDA06_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4229546.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5046..>5449)
                     /locus_tag="SDA06_002292"
     CDS             complement(5046..>5449)
                     /locus_tag="SDA06_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEG4229547.1"
                     /translation="EKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1608 a         1078 c          928 g         1835 t
ORIGIN      
        1 taaatccttt cataacaaaa tatatacttc ctattttatc atgaaaaata tctgttaagc
       61 accaatgatt cggttaaata agtttaataa cacatctttc acgccagctg cattgaacac
      121 aagtccgacg gcgattagag caataatcaa gaagccaatg agttttgaaa attctcgttt
      181 aaaacctaaa aacagtccaa tgacaacaat cgctaacaat accaatgact gtgcattatc
      241 caagaaccag ttgtacaaat tctgcccaaa attcatgtga ttgtcctcct ttccttaaga
      301 tatttttctc gataatagat atagcttttt acacgcctta ataattgaaa aggggttaaa
      361 tgctctccat agacggttac tcgattggta taattattat aaaatctcac cttgtaaaaa
      421 tcataggtat agatggtatc accagcttgc cgttttccct caataatgag agacgtatat
      481 ttccctgtat tttgtatata cgcttgaaac tcaatgccac actgaaaccc taacttcttt
      541 aattcttttg ttaaaaggct catgtaatca aatcctccat ggctagatgt tgttgttcaa
      601 ttaatttctc atgtttttct gtcagtttgg cttcctgaat catggtggag ataatggttg
      661 taccgttgag aacatctaaa atgcttgcga tttttaatgt aggggcgact tgatgatgta
      721 accagttcaa tgttcgttca aaagaataag gctctggctg tgtggttagg cgtaatgctc
      781 cacgattttt accgataaac cattcccaac gttcattagt tttccaatca ctccgacgtt
      841 tagtgtcgtc tttatctgca aagcggatat agcgattgat aatttcaaag gctgtttttt
      901 ccgcctgttg atgtgccaat aaatctttca tggcttgggt ggctcggtca ttttttaacc
      961 gaatttcaaa ccgattcttt acttctgcgt cttctaaggg aacatcattt ttcacgtatt
     1021 gctcataatc tttctcatat atacagaaat agacttcact ttttaatgag ccaatataaa
     1081 gtgtgtttcc catgtatggt ttttcatcac ggtgaaccaa ttcaccactg cgataatttt
     1141 taaagcttcg aaagacggaa atgcattctt cctgtttaca tttctttgcc aaatctggaa
     1201 tattcaaaat ccctgtttta tcgttgatag ctaaatccaa gcgtttgaaa atacctttat
     1261 gttccatgca atcttgaaag aaatcatacc agctccgatg ttgagccaat agaaagtttt
     1321 caaattgccg acagcctttt cctttcagtt ctaataaaac acctttactt acatcatgag
     1381 agactaggac aacaatatca ccgaaacgat agtgttcggg ataagaataa tagccaaagt
     1441 cttcgtgtag catataatcc atattcaaac gtaaaacctc accgataatt tgagccacat
     1501 cattggtagg gaatcgaatc cgtacatagt cgaataacat ctccaatggg ttatcgggat
     1561 tgaagcgttc caaagcgttc agtaaatctt ctttcacctg ttgagtaggg actgccttgc
     1621 ctgtttcaat atccgatagg taaggtctgg taatatgaga agcaaccgcc agtttatttt
     1681 gagaaacgcc ataagccagt cgcttttctt tcaattcctt aatccaaatt ttttcgtttt
     1741 ccattcgcaa acctccaatc taaaaaagat gtaaactatg acccttgagt ttacaccttt
     1801 catattttcc gcaatccttt gtaccatcaa cattggttga tgagaattgc ctgttttcag
     1861 ttgtatttgt gcccccctgt tagatagagg gggctaaagg attggcgtgg ctaacgccac
     1921 accaatcaaa gcaagtctga acctgtacct ttcacttcgt gcggtacagg ctcagattgc
     1981 tttttttgaa tgacttttcc tatttctcct aagaaatcgt acctttttgg tacaaggggt
     2041 gtataaaact cactaatcac actttttcct gtatccacgt aacctcttcc cttgatgggt
     2101 tttaagaaaa attgtttttg tgtatcgctt ccgaacatca taccaaagcc catttcagac
     2161 atacgaccca aagccacacg gaagttaaag ttgtcccgaa tcccatcact aaaatatttt
     2221 gcgtctggac gttgacaggc aagaattaaa aagaagcctg cttgtcgtcc taacattaca
     2281 atttgtttga gtttatgaat aacggtggta ctttcacgac ctagcatatc catcattgcc
     2341 acatattcat caaaaataag aaaattggca gacaaaccaa gataagcata attttctcct
     2401 gttttatagt tagggtgttg tttcatggtt tcactgcgat caatcatatc ttcgtaaaat
     2461 tgattgatac atgccaacat atcttcttta cggtaataga cattattcat gaccgcccct
     2521 aagtctgcca aatcagcatt tttagggtca agaatataaa gctttgcatt tgtatgcagg
     2581 agagattcaa tcaatgtgag aataaaatag gattttccac taccagtgcc cccagcaatt
     2641 aacatgtgag ggagagaatc gaactcccaa tagaccgatt ccatcaattt gacacgtcca
     2701 tcttgagcta ccacgtcttc aatgccaatt cgattgccaa tagtatcata aagtaggaca
     2761 tactctacat aagaatcttt caacgttttt tccactaatt cacaatacaa tcctgtttct
     2821 agtttgtttt ctaggtgcaa aagctgatct tgatatttcc ctaaggtgat ttctacatga
     2881 atatggatta gtccatcttt cattcgataa tatattttag gaaaatggcg gattcgttgt
     2941 ttggttttct tgctgggtaa atctttaaag aatccctctg attgagtttc ttcggcttca
     3001 taccaatgat tttctaaaat catacgagct aacttttgac ggtgctctaa ctgttttata
     3061 gaatctatac gatagtgata ataaagccag ctgataagtc cgcatacaaa aagagcagtg
     3121 agaatactgc tccaaaagta tggcgtatga agttgataga aaaatgtatc taactgaact
     3181 gcttgccagt tcaccgattg taacgctctc caatgaaaag ggagtaggac gactagaaaa
     3241 actaccagca atgaaattag tacaaaatga tagaccaagt gcttatcgtt ggctttgatt
     3301 cgctctcctc tgtcatgaag caatggcata aaaatcctcc tttccaccta tcaaaataat
     3361 tgtgtattgt agatacgagc ctgtttatca aaagttggtt gagaaatggc ttcatatttc
     3421 tgataagcga ttcgcttttc aaagcgtaaa tttttttctt tcggataata atgacgtcga
     3481 tagagagtgt gtcctaacag ttttttcgct tcttttgtca acggatagag atagcgaaac
     3541 atgcgtccat tgattttctc aatccctttg tattcgcaaa atgcctggga aagccaatgc
     3601 tttttctcta cctgttcaaa gcgagcattt tcctctaata aaaggcgagc acttcgaggg
     3661 tgtatctttt cccatgactg cttatcacga tagacacttg tcttgaaata cccgcagtaa
     3721 aagaagttgg acgcttgata gacatagcca cattttccct caattccatc tgccagtgta
     3781 tataaaaaat gacagtccgt atgttctttc aaccacttaa ttagagctga aagagcttga
     3841 ctgccaaaat aattggtttg attcatttct ggaagaaaac acatctttcc aatttctaaa
     3901 taatctgtgg tttgaaggga actgtcggga aagagtttgc gtatggtttg taggggttgc
     3961 gtaccccagc ctaattccac aacgcctaat aatttttctt ctgaaaaaat ccctagaaag
     4021 tatttacaca atctagggag tatctttgaa taatggtatt gccgaataaa agaaatggct
     4081 tcatccttcg gaatttcttt caaaatcaat ggtgtgtttg tcataagcaa cacctatttt
     4141 tcactttcgg acgcttcttt tttatatttc tgatgaggtt ttgtctgtcc ttttaataca
     4201 agatcgtctg ctttcacgta ccaatcaacg tctgcccctc gaaaagtagc cgtcgctacg
     4261 gtatctgcca caggattgat taattccact tctgcgttgt agtcaaattc tttcaaagga
     4321 acagaagcgg ggatactgac ttgtatcatg cgtccttgtc cttttgattt taagtcatag
     4381 gtacgttctt taatttcttc ggaaaccgta ccatcttcat tctggatacg aacctcacga
     4441 cgcaaggcgg aaaatttcaa tgtgccaaat gtctggtctt tttcaatcac aataccttct
     4501 gctaatctca tattttttca cctttccttt cttatgcttt caccatatcg tctgcgtgca
     4561 taatgtaatt ggtaaatcct cgattgccaa ttttataacc ttctgcggta atatgtgggt
     4621 tgatgagttt gactttttcg tcggattcaa aatgtttctc gccagcttcg gagggaagaa
     4681 tgacaacaat atcatctgct cgttgaatcg ttgagtataa attgtaacta cgagagagaa
     4741 tggttggtct gccatttatt cgacgttgtt caatgttccc ttcaccagca tattctaatt
     4801 gtccaaatgt tttttccata ttgggaatga catatttaag ttccatattt ttacctatcc
     4861 tttctgtttt tatgtttgaa tgaatcttat ttgtgtgatg gttttaaagg ggggagagcg
     4921 accttttgat tcttgatttt ttattttcat aagttcactt cctttcaaaa ttgggtaaaa
     4981 aaatagacgc tccgtactga gcgtccgatt cctactatat ttaaaacaac gactacctag
     5041 gtattttaaa tattataatc aaattctatt ttatctaata attgagtggg ataaagcaag
     5101 tctcctttac tcgtaaaata acccacctct gagaaaagaa ataaattatt ttttgctctt
     5161 gaacaaatta cgtatagtag ttttctcgct gaactaattc ctttatcaga aacattaaaa
     5221 atttcattcc aatgaggaat tttcccgttt agaagattaa tagcgataac agtatcatac
     5281 tcatttcctt taactttatg tgctgtagta agtgtgatac catctcgaga tttaaacatc
     5341 ttcttaaaac tagatacact actagataat gaattatttt ttatccgatc ttctacttcg
     5401 ttaataaaaa atagatagtg cttaaaaata gacgggtaat ttttctcgt
//
LOCUS       DARDHD010000157         5283 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000157 DARDHD010000000
VERSION     DARDHD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.160"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..1823)
                     /gene="glmS"
                     /locus_tag="SDA06_002293"
     CDS             complement(18..1823)
                     /gene="glmS"
                     /locus_tag="SDA06_002293"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG4229548.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2232..2522
                     /locus_tag="SDA06_002294"
     CDS             2232..2522
                     /locus_tag="SDA06_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229549.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2535..3560
                     /gene="sppA"
                     /locus_tag="SDA06_002295"
     CDS             2535..3560
                     /gene="sppA"
                     /locus_tag="SDA06_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4229550.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3563..4222
                     /locus_tag="SDA06_002296"
     CDS             3563..4222
                     /locus_tag="SDA06_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4229551.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4379..>5283
                     /locus_tag="SDA06_002297"
     CDS             4379..>5283
                     /locus_tag="SDA06_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229552.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREK"
BASE COUNT         1649 a          957 c         1115 g         1562 t
ORIGIN      
        1 tggtccttgt tcttttatta ttctactgtc actgatttcg ctaaattacg tggtttgtca
       61 acgtcgaatc cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg
      121 gttagagtag ttagataagg gtggacagtt ggtagcacca gctggtcact ttccttttct
      181 aatccctctg agacaataac tagtgtttta gcgccacggc tttcgacttc tttcaggttt
      241 cctcgagtat gcgcaccagt cacttcttca ctgatgattc cgatcactgg tgttccttct
      301 tcgatcaaag caatcgttcc atgtttcaat tcgccagcgg caaaaccttc tgcctggata
      361 taactgattt ctttcaattt caaagcagct tcaagagaaa cgttgtagtc agcaccacgt
      421 ccaatgtaaa aggcatttct tgtatcgctg agatatactt ctgccaactc ttctaaataa
      481 tctttttggg cgatgaccga ttccatcgca ttagcaacta aactcaattc atgagtgata
      541 tcgaatacaa gagaagtttc aaattctttt ttatcaccga tagctttggc taataaagtt
      601 agtacagcta tttgtgcagt ataagctttc gttgatgcta ctgcgatttc gggtccagca
      661 tgcaatagca gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac atttgtaatc
      721 gtaagtgaag ggtagccgag acggttgatt tttaccagca cttggcggct gtctgctgtt
      781 tcgccacttt gactcaagaa aataaagaat ggtttttttg aaagaagagg tgtgttatag
      841 ccaaattcac tggctaaatg aacctcaaca ggaatctgtg tcaatttttc caaagtctgt
      901 ttacctgcta accccgcatg gtaactagtt ccgcaagcaa cgatatagat tcgatcgcta
      961 tcagacaatg tatcgaccag cttttgatca agagagacac gattgtcttc tgttacgtat
     1021 ttttgagcga tacgacgcat gataataggc tgttcgtcga tttctttcag catataaaaa
     1081 ggataggtac ctttctcgat atctgaagca tctacttgtg cttcgaatga agaacgttct
     1141 acgatgtcac ctgcaaaatt ttgaataata actttttctg atgtgacggt aaccatctct
     1201 tcatcttcaa tctcaacgaa ttccttcgta tgagcaagca tagccattgc atcgctggca
     1261 atcacattga atccatcgcc gagtccgatt aatagtggac ttttattttt agcgacgtaa
     1321 ataacatcag gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt gatgacccgc
     1381 aaagctttta aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc aattagatga
     1441 gcgataatct cagtatcggt ctcaccaata aagtggtcat ttgctaaata agcaatttta
     1501 agttcttcaa agttttcgat tacgccgttg tgaacgagca cgaagcgtcc actttgggaa
     1561 gtatgtggat gggcgttttc ttcagaaggt tgcccgtgag ttgcccatcg tgtatgacca
     1621 atacctatat ttcctgtagt atcaatattt attttgtttt tcaaattttg aattctgcct
     1681 tgtgctttga ccagataatc agttgtgcca tctgagacaa agataccagc tgaatcataa
     1741 ccacggtatt ctaatttttc tagaccgtca atcaatcctg gtgttacatt ttttaatcca
     1801 atcattccaa caattccgca cataaaaaac atcctacttt ctataatgaa ttcgtatgaa
     1861 tgcttccagc tgcaggaaaa tccttttgtt ttatctttgc agacattttt tgtggatttt
     1921 ctgtagagtg cagtccctag ggccatccgc cgaatctttc gataaaccct ctcctcgtca
     1981 tctgtttaca cagacctggc gctatcctaa aaggtataac tatcctcctt tttgtttttt
     2041 atctggaaac atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa aaaaagaata
     2101 aaaaataaat tggtatatat caaattcttt gtcaaatgtc ctttttatgt caaaaaataa
     2161 tgggttttta tctatcatct ttttcaggag ttcggtacaa tagaaagtaa tcggatagag
     2221 gaggagtaaa aatggattca aaaattgaga tcatgacact gggaatgtta aaaaaacaat
     2281 tgtccaaatt cgaagcatca gctggtgtat cagatgatac gaaaatattt ttagatactg
     2341 gctgggatag catccaagaa atcgcacctg atgccttgga agttgctcaa gctcgtgagt
     2401 ttacggtaga agatgaatta acaaaagagt cgttttccgg gtatgcgcgg gaagaaaaag
     2461 cagagcggtt cgacacttct gagcagtcag aaactgtgat cgtcataaaa aacttgtatt
     2521 agaaaggaga aagaatgaat aaaagacgtt gggcagcagt tctgattgct gcaggattat
     2581 ttgtggtttc gttagtttca gctagtttga ctaagcctca ggaaaacgat caaattgagg
     2641 gattgaacag ttggttatat ggggatgaag aactagctcc aaatgtgttg gaagagggag
     2701 acagtacgca aaaaatcgta aaattatcag tagatggaac gattgctgcg ggagggtctt
     2761 caggtttatt tgcttctgaa ggttacaatc ataaaaattt tatggaacaa ctgaaggcga
     2821 ttgaagaaga tccaacagtc aaaggtatct ttttagaagt aaattctcct ggtggcggtg
     2881 tctacgaaag tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa catgatatcc
     2941 caatgtatgt cagcatgaaa aacatggcag ctagcggtgg ctactatatt tctgctcaag
     3001 cagataaaat ctttgcaaca gaagaaacag tgaccggctc aattggtgtg atcatgtcag
     3061 gattgaatta ttcaggactt ctagaaaaac tgggtgtgga agatacaaca gttaaaagcg
     3121 gagcgttgaa agatatgggt tcaggaacgc gaccagagac aaaagaagaa gaagcggtgc
     3181 ttcaagcata tatcgacagc gcatatagtc gttttgtagc aatcgtcagc gagggacgga
     3241 ataaatctga agaagaagtc aaaaaaattg ctgatggaag gatttatgat ggtgtccaag
     3301 caaaagaagt aggtttagtc gatgaattgg gatttcctga agatgcttta aaagctatac
     3361 gaaaggaaca acagcttgaa gatgcggaac tagtagaata ttcttccacg ccatctgggt
     3421 ttgctagtac atggttagga agtaaactag cagaattcca gggattgaag ccttctgaga
     3481 ctagtcagat cttatccgtt cttgagaatc tagggacagc ggaatcacca aaagcaatgt
     3541 attattacgg aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg aacaagaaaa
     3601 agataagcaa cagcagcctg ctgtaggacc tgactccaac ttttcccaaa aggtactgag
     3661 ctcttttgaa agagagcctt tgacgcccga agaaatcaaa aagaaacaac gtaaatggaa
     3721 aaagtatacg gacacaagaa atgaaccgcc aaaagcaaat gattttccta agtatttcta
     3781 tgcaggtttt tttatccgaa tgtttgcctt cttggtcgat cttttgtgta tcggagctat
     3841 tacgcgttta acacttggta ttgccaaaaa tctaggttgg cttaattttt cagattctta
     3901 tctgagtctc tacggattca cagccttact tatttatcta gcgtatttta ttttattgac
     3961 gaaattcaat catggccaaa cgattgggaa aatgatcttt ggtattcgag tcatcagctt
     4021 tgatgaagaa gaacttagct ggtcaaccgt tttagttaga gaaggtgctt gccggttcat
     4081 tctaaaattt cctttgctga tgttagggta tcttccagca gtctttaatc agaaaaagca
     4141 acatctgggc gattttttca ctaatacaag tgttgttacg atcaatctga tcaaagcgtt
     4201 caaccaggaa gtcaacagct gatgagcaat aaaaagcagt aacagttgaa agcaccttct
     4261 aaaagtcaga tctaaaatct agcttttaga gggtgcttca gttattttta attgtcatgc
     4321 tttttttgtt ttgctactat tgaacatgag ctgaatatga gcaagaggag gacactgcgt
     4381 gaagccgatt gattttccag aaaaatatga gaatttaatg cgagtggcac aacaagcact
     4441 agcaaaccag caatatcagc aggcaaaaga attatttcaa cgagcttacg agttgaaaga
     4501 aagctttgag gctaatagcc tgcttgtttt ttgtctttac gagttggacg agaaaaaaga
     4561 agcattgaag caagcactct tgcatgaaaa acagtatctt caaaatgagg agtttgctga
     4621 attttatttt gacttgttaa tcagtacaca agatttttta tatgctagaa aattgatagc
     4681 aagtacagat ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc aatttgcaga
     4741 agaattatcc ggacaaatgg agaggcagaa gatcaaggca ctccacaata aatcggagga
     4801 acttccttcg ttggaacctg cacgtcagct tgcgtctatt gatgagatcg aacagcttcc
     4861 ttatcatgag tttatccaaa cagcgagtaa acttattgta tcgccagagg tccatatttt
     4921 agcaagggca aaattactgg agacacttag gcaattgaac gaatgccatc ctgttttcta
     4981 tttgacgatt gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa agccgcagca
     5041 gcaggcaagt taccagcaat tatgtgtatt ttcagatcgt tacgggaatg aagatgcgct
     5101 gcttagttcc gtgttaaaag aagaattcac gttgcaatct gcaattgttt atcctgtcta
     5161 tgatacatac atcgaagacc cgaagcgctg gtttcaactg actgtagagg cgtgtacagg
     5221 aaaaacgatg tatgaaggta cagaagatga aaaacaagat tttttaaaaa aaagagaaaa
     5281 aat
//
LOCUS       DARDHD010000158         5236 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000158 DARDHD010000000
VERSION     DARDHD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.161"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..1087
                     /locus_tag="SDA06_002298"
     CDS             2..1087
                     /locus_tag="SDA06_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4229553.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1084..2976
                     /locus_tag="SDA06_002299"
     CDS             1084..2976
                     /locus_tag="SDA06_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4229554.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2983..3360
                     /locus_tag="SDA06_002300"
     CDS             2983..3360
                     /locus_tag="SDA06_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4229555.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3514..4296
                     /locus_tag="SDA06_002301"
     CDS             3514..4296
                     /locus_tag="SDA06_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4229556.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4422..5153)
                     /gene="erm(A)"
                     /locus_tag="SDA06_002302"
     CDS             complement(4422..5153)
                     /gene="erm(A)"
                     /locus_tag="SDA06_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4229557.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1894 a          720 c         1010 g         1612 t
ORIGIN      
        1 tgtgaaggtt caaaggatag aagtggagaa taagccgtat ccattgtatt tattactaga
       61 taaagaatac cagctaatag aaccagtaat gaaatttatt aaatacttag ataatactgg
      121 taagtctcct aataccatta aagcatactg ctatcattta aagttgctgt acgagttcat
      181 ggaacagaga ggtgttattc ttaatgatat taactttgag ttgttagcag acttcgtagg
      241 ttggttgaga tatccttcag catcaaatgt aattgatctt cagtcaaaaa aagccataag
      301 agaagaaacg acagtgaata caattttaaa tgtagttatg agttttcttg attatttaag
      361 tagattagga gaatttaaat caattgatgt atttaaacaa gcaaagggaa gaaatttcaa
      421 aggattttta catcatgtta ataagggtag ataccaaaag aatgtcttaa agttaagggt
      481 taaaaagaaa cagataagaa cattgagatc agaggaagtt aagcaaatta ttgatgcttg
      541 tcatacgaaa agagataaat taattttaat gcttatgtat gagggtggtt taagaatcgg
      601 tgaagtgtta tcgcttaggc ttgaagatat tgtcacttgg gacaatcaaa tccatttaac
      661 acctagagat gttaatgtta atgaagctta tattaaatta aggaaggaaa gaacaataca
      721 tgtgagtaaa gaacttatgt cactttatac agattacttg atatatgagt atagtgagga
      781 attggagcat gattatgttt ttatttcctt aaaagaaggc tattttggga aaccactaaa
      841 gtaccaaagt gttcttgatc tagttagaag aatagttaaa aggactggaa tagaatttac
      901 atcacatatg cttcgccaca ctcacgcaac gcagctaatt agggaaggat gggatgttgc
      961 gttcgttcaa aagagattag gtcacgcaca tgttcagaca acgttaaata cctatgttca
     1021 tctttcagat caggatatga aaaatgagtt taataaatac ctcgagagaa aggagcataa
     1081 gaaatgaatg cttctagtaa aaggaaaatt attagtcaga gtgagattag caaaaaaata
     1141 gctgtaatga atgaagaaat gcaggggttt tgggctaata atagttggga tataagaaaa
     1201 tgtccacatc cttctgccat agaattaagt aagaatcctg ctttaaggaa tcgttgggtt
     1261 cgttttgaac gtgttaaaaa tctgtggtta agaacagaat tgaaatattt ttatttttac
     1321 catttaaaca atggaatatg gaatgcaaaa actgtctgga ttagaaaagg aacagtaatt
     1381 aataaaatgt tagatttctt agatttaaag tatcctagca ttacttcaat tactgaagtt
     1441 cctattgaaa aagcaatgac ggagtataga acttatttga caaaacgggg tgttagaatt
     1501 accactacta attataagat tactgctaat caagaaaaaa cacctgtaaa agctaattcc
     1561 tactatgtta ctaatctaaa acaatttatg gagttttatg agaactttta ttttgatgga
     1621 gaggagtggg ataaagacgt ttgggataga cgtaacttac ctttgccaga tgataaggtt
     1681 aacccaacac aatatgaata tacaattaac tttaaagggt ttcggaatac atattttaaa
     1741 caacttgtaa aaagatattg taagttgaga ttgaacatgg atagcttttc ctatgtaagt
     1801 gatattgccc aaagacttaa agagttcttt aattttctgg acatgaaatt taaacaagtt
     1861 cagagagtac accaattaac gagagtggaa attgaagcat atttaagtga actaaacatg
     1921 atgggaataa aacctagtac aataactggg aggatctcta tattggaagg actatttagt
     1981 acccttctta ggctagaatg ggatgatgtt ccttccaaaa tattaattta ttctgaggac
     2041 tatccgaaaa taccaagagc aaaaccacgc tttatagatg aattcgtcct agagcaattg
     2101 aacagtcatc ttgataaatt acccgaatat atagctacga tgactatgat tgttcaagaa
     2161 tgtggaatga ggataagtga attgtgcacc ttgaaaaaag gctgtctatt agaggacaaa
     2221 gatggagatt tctttttaaa gtattatcaa tggaaaatga aaaaggagca tatagttcca
     2281 atatctaaag aggtagcttt acttattaaa gttcgggaag ataaagtttc agaggaattt
     2341 ccagatagtg aatacctctt tccaagaaaa gatggatcgc cattaaaaca agaaacattt
     2401 agaggtgagt taaataaatt agcttatgag caaaatatag tggataaatc aggtgagatt
     2461 tatagattcc atgcccatgc ctttcgccat acagtaggaa caagaatgat taacaacggg
     2521 atgccccagc atattgtgca gaaatttttg gggcatgaaa gcccagaaat gacaagcaga
     2581 tacgctcata tctttgatga aactctaaaa aatgaattta ctaaatttca ggaaaaactg
     2641 gttaccaata atggagatgt gcttgatcta gatgaagata atgaagtcga tgatgtagag
     2701 cttcaatggt tcaagaaaaa tataaatgca caagtgcttc caaatggtta ttgtagattg
     2761 ccagtagtag caggtggttg tccacatgcg aatgcatgct tagattgcac tcacttctgt
     2821 accagtaagc aattcttacc acagcacgaa gaacagttag agcgtacaga agagttatta
     2881 gccatagcta aggataaaca atggcaaaga caagtagaga ctaatagccg tgttaaagag
     2941 cgtttagaac aaatcattgg aagtttgacg gggtaattat caatggataa acaagttaga
     3001 aatacaacag aaattgtacg tttggcgaag cagaaatcaa aaaagacaag ggaaaaagta
     3061 gacaaagcga tttctaaatt ttcgattgaa ggtaaagtta ttaattttaa ttcaatagca
     3121 aaggaagcta atgtttctaa atcatggctt tataaggaac acgatattag gcaaagaatc
     3181 gaatcccttc gtgagcgtca aataacagca aatgtagtct caaaacccaa gaaaagttct
     3241 cgttcggagg aaatccttat taaaacctta aaaagaagag taatggaatt agaaaaagaa
     3301 aataaaaaat tacagaacca aattcaaaaa ttatatggag atctgtataa taaagaataa
     3361 ttattaatct gtagacaaat tgtgaaagga tgtacttaaa cgctaacggt cagctttatt
     3421 gaacagtcat ttaggtatat gtccaatcta gggtaagtaa attgagtatc aatataaact
     3481 ttatatgaac ataatcagtt acgaggtgaa atcatgagca atttgattaa cggaaaaata
     3541 ccaaatcaag cgattcaaac attaaaaatc gtaaaagatt tatttggaag ttcaatagtt
     3601 ggagtatatc tatttggttc agcagtaaat ggtggtttac gcattaacag cgatgtagat
     3661 gttctagtcg tcgtgaatca tagtttacct caattaactc gaaaaaaact aacagaaaga
     3721 ctaatgacta tatcaggaaa gattggaaat acggattctg ttagaccact tgaagttacg
     3781 gttataaata ggagtgaagt tgtcccttgg caatatcctc caaaaagaga atttatatac
     3841 ggtgagtggc tcaggggtga atttgagaat ggacaaattc aggaaccaag ctatgatcct
     3901 gatttggcta ttgttttagc acaagcaaga aagaatagta tttctctatt tggtcctgat
     3961 tcttcaagta tacttgtctc cgtacctttg acagatattc gaagagcaat taaggattct
     4021 ttgccagaac taattgaggg gataaaaggt gatgagcgta atgtaatttt aaccctagct
     4081 cgaatgtggc aaacagtgac tactggtgaa attacctcga aagatgtcgc tgcagaatgg
     4141 gctatacctc ttttacctaa agagcatgta actttactgg atatagctag aaaaggctat
     4201 cggggagagt gtgatgataa gtgggaagga ctatattcaa aggtgaaagc actcgttaag
     4261 tatatgaaaa attctataga aacttctctc aattaggcta attttattgc aataacaggt
     4321 gcttactttt aaaactactg atttattgat aaatattgaa caatttttgg gaagaataaa
     4381 gcgtcctctt gtgaaattag agaacgcttt attactttaa tttagtgaaa caatttgtaa
     4441 ctattgaaaa tagaaagaaa ttgttccttc gatagtttat taatattagt gacatttgca
     4501 tgcttcaaag cctgtcggaa ttggttttta gtgaaaagaa cacgatattc acggtttacc
     4561 cacttataaa caaaagatcg atactttttg tagtccttct ttgaaatcaa tggttgatgt
     4621 cgttcaagaa caatcaatac agagtctaca cttggcttag gatgaaaata tagtggtggt
     4681 acttttttga gcatttttat atccatctcc accattaata gtaaacccaa agctcgttgc
     4741 agattttgca atcttttcgc aaatcccttc tcaacgataa gatagctata tttagcctga
     4801 ctttcaaagg taattctttt gacaatatcc gtactgatgt tataaggaat attaccatat
     4861 atcttatagt ttatatgttt tgggaaggaa aattttagaa tatccgtttg aatcactttt
     4921 atattctcag aggggtttac cgcttcttta gtcacttgac ataagcctcc atcaatttct
     4981 atagcagtaa ctgatcgact cattttgact agctctttgg taaaatgtcc ttttcctgat
     5041 ccgatttcta ttacgttgtc ttgtttactg atattcgtgt gattcaatat ttcttttaca
     5101 tgctttttag aagtaataaa attttgcgtg tctttagggt ttttctggtt cattataacc
     5161 ttctccttac tggttataat gaactgacat taagcgttca ttataaccaa ttaattttga
     5221 tttggttgat aacgaa
//
LOCUS       DARDHD010000159         5186 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000159 DARDHD010000000
VERSION     DARDHD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.162"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..519)
                     /locus_tag="SDA06_002303"
     CDS             complement(61..519)
                     /locus_tag="SDA06_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4229558.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(532..1863)
                     /locus_tag="SDA06_002304"
     CDS             complement(532..1863)
                     /locus_tag="SDA06_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG4229559.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1874..3316)
                     /locus_tag="SDA06_002305"
     CDS             complement(1874..3316)
                     /locus_tag="SDA06_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4229560.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3303..4067)
                     /locus_tag="SDA06_002306"
     CDS             complement(3303..4067)
                     /locus_tag="SDA06_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG4229561.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4171..5046)
                     /locus_tag="SDA06_002307"
     CDS             complement(4171..5046)
                     /locus_tag="SDA06_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229562.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1576 a         1098 c          755 g         1757 t
ORIGIN      
        1 caaaaaaatt ttgtaaattc acccataaat atcaccctat ttaacttttt tcatcgtaca
       61 ctaaaattca aaacaactat ctttaatctt tacttcttgg ttttgtttta ataaatttcc
      121 ttcaaaaatg cctggagaaa ctacaatgat caccgtatct ttttgtgctt gctccaactt
      181 cttcaaatct ataatagcta acagtgtccc ttcttttacc tcctggttac tttcaacccg
      241 tatctgaaac ggacttcctt cgagttcaac tgtatctaaa cccatatgga tcagtatttt
      301 tctgccagat tcactttcga tcgttatggc atgtcgggta ggaaatatat tatcgattat
      361 tcctttaact ggcgcatgga tatatccatc atgattcgtt atagcgtatc caactcctat
      421 catttttgaa gaaaagacct cgtcttttac tgctgttaat ggaataattt gaccactagc
      481 tacagctttt agaatgtttc ttgttttaaa gaaatgcact ttttatcacc tctactttaa
      541 tgctggccaa tattttttgt ttactttgat catttcatct agtatttttt ttgcgactct
      601 ggcatttggt acggtttgat tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc
      661 aaagtaagca tctactagta acttctctac tgcaacttgt gcctccatca atcctttatg
      721 aaaatcactg attggttttc ttagtgtaat tggctccaca ccagttgcac caacatatgc
      781 cggtatttca acaactgcat ctgaccgtag attcggaatc gccccttgat taggaacgat
      841 tagcataaag cgacgacgat catcattcaa caaggaaata gctatatcga cgatatattg
      901 tccatgctcc ccaaaattaa agttcaatac ttcttccaca ataccgcggc gtattttatc
      961 agccatttct ttggtatttt tctctcgtcc atccatgacc atattagcac gtgtatactc
     1021 tttgtcagta tgctcaacga ccatatcagg atataagtaa tactctaagt aattattcgg
     1081 gatattactt ggaaaatttt tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt
     1141 tttatctcct atattaaaat cggcgacttg catttctttt gttattagtt tttcaataat
     1201 ctctggcata atttctcgtt gcaattcttt atcataaatc gatgtatacc agccaaagtg
     1261 gtttaaacca taatacgtaa cgatccaatt cttacgatca taaccgtaat tcactgcaat
     1321 cgtttcttca attgaaatag tcatatcaca ggcattaatc atttttacat tcggatattt
     1381 acgacgaaca gcttcagaaa cgatcgactc tggatttgta taattcaaaa tccatgcttt
     1441 gggggcatag tcttgtatat gatcgacaat ttccaataaa cctttgattg agcgcatccc
     1501 ataagcaaat cctccgagtc cacacgtttc ttgtcctact aatccatgtt taagtggaat
     1561 cttttcgtcc ttttctcgca tctccaatcc tccaacacga atttgtgaga aaacaaaatc
     1621 gcatcctgta aaagcaagct ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc
     1681 ttttcttttc agcataaatt taacaatcaa gtacatatcc atatttcttt cttcatcaat
     1741 atcatataaa cgtatttcag ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac
     1801 gattcctggt gtatacgtac tcccgcctcc agctatagtg ataatctgtt ttttcatcat
     1861 catcttttcc tttctattct tctaataatt tattgaattc tgccataaca taagtcactt
     1921 ctaagccgat gataatttga taattttttt cagttggttt aatgatcccg cttatcccta
     1981 attgcttgat tcgctgttcc tcgaccatgg tgggatccac tacctctacc cgtaggcgtg
     2041 tattacaact atagacatcc aggatattgg cttttcctcc aaacgcttgt agcatttttg
     2101 ttgccatata ttcataattt ttacttgcta accctaactc tgtttcttct aaggttactt
     2161 cattgccaaa ctctgcctta gcttcacgtc ctaatgtctg aatgtttctc ttattgatca
     2221 aataataaaa agtaacgtaa tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa
     2281 tagcattggt agcgattcga aaattgatga gataatcaac aatacatgct ccgaatgtaa
     2341 aaccaattcg tatattgaat aagtaacaga caatccctgc taaaccggca aaaagtgcat
     2401 ggatcacgta caacatagga gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat
     2461 ttgcaataaa tgaagttcct gcaccgcttc ccatgattcc tttgaccatc tctttatttt
     2521 ctttataagc tgctttatag atagctagac atatagctgg tataccaaac atcattacta
     2581 caaaaaaaac tgataaaaac agtccagcct gaggatctcc gttgataaag cgtgtcatct
     2641 ctcctctgaa aacttccttg ctaggagatg tatagcttcc taaatcgtaa taaatatacg
     2701 tattcaacac atggtgcagt ccaaagggga tcagcaaacg attcaaaaac ataaatatcc
     2761 ccacacctaa tgctcccata tcaacgagca gttttgcaaa attatcaatc ccaatttgga
     2821 tgagcggcca gataaagccc aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag
     2881 tcaatgggaa tttttcgccc gcaaaataag aaaaaactac tggtaatttt tgttctttaa
     2941 agcgattata cgtccatgcc gctactaaac cggaaataat tccaccaaaa atccccatat
     3001 tcacttctgg gtccataatt tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag
     3061 taagtgcagg tattcctttg tccttatttt tgacaaaacc gattgcaact cctatagcaa
     3121 ataagatatc caagtttcca aaaacggcat ttccagctgc accaattact gggatgttca
     3181 gcatatcgtc agctgataaa cgcatgatca atcccgctaa tggcatcgct gcaactggaa
     3241 gtagcatcgc tcttccaagc ttctgcacat ttgatttaac attcattttt attcctcctc
     3301 tattaaatat cctcatatgt tactaactca atcaaaggat catttttcac tttttgagca
     3361 aatgctgaat gaattaaaaa ttcgacttgg tgggtacgag gatacacaaa acttgaacca
     3421 tttaacaatt cctgatccaa atacgctgcg tgacacatga cttcgatcgt tttatcttct
     3481 ctcaatatca tgagtaaact tgccaaatat tcttccacac tcgtattgat ttgtcgtttt
     3541 ttttcatcag tttctcctac gacatcgaaa caaggctcaa aataatcgac atgacgaact
     3601 tcatcttttc gttcaaagtt gctccgaacc ggcaaatcat atttttttgc taatgtaaca
     3661 actacctcct gattttcatc caaagtatga gtatgatgat gactgtctaa atgagttggt
     3721 atgatccccg catgataaat tttttgaatc tgagcattcc attcctgata cagttgatct
     3781 ttgtcaatgt taaatggatg ctgataataa gataaggatc tgaactctcc gctttcgata
     3841 agagtatcga cagttttgag gagtggtttg ccacacgtca atgttaaatg gactcctaca
     3901 cctagcctag gcatttcctt tcttagtttc acagcatgct cgaaacccgg catatttgcc
     3961 atcaatgtag ttgatgtcaa aattcctcgc tgataagcat ccataatccc ataattcaca
     4021 ccatggctat agccaaaatc atccgaatta aaaatcactt ttcccatttc attctccttc
     4081 ttttttctac taaaataggc gcaaccaaat aatcatagtc catttactat gtgtcatttg
     4141 gttgcgccta tagatagtta cgcccttatt ttaatagatt cttgctagat gaattgccaa
     4201 atagatgatt tcattttctt gaatggcaag ttgaaattgc tgttcgatga tatctttaat
     4261 ctttagtgct gtacaatacg ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt
     4321 ttctactggc gtttcttcct gataaatcaa gcgtttaacc aggaatttga tatgaaccat
     4381 taagcgatga taagtcagtg attctttatc ataaacgttt gaataggatt tttctagatg
     4441 ggacaaaatc gtggtcgtca tttctactat ttttaagctc tttaaactat taggctctcc
     4501 tagctcagca ttaatgatat gcatagtgat gaatcctact tcatttgacc ctaactcggt
     4561 ttgatatcga tgattgattc gctcgaccac ttctactgct atatcatatt catttggata
     4621 aaggaatctc atttctggca acataattgc tgtgacatct ctatcttctc tttttcgttg
     4681 aatagcaaaa taaatatgat ctgtcaatgt aatatagata tttgaatcta atggagagga
     4741 gatcctagct gttatcagtt caatcacttc ttccgcaaaa tccataaatt cgattggaat
     4801 ctgcgagact atctcactga atcgtttgat cttttcttta gaatctaata caaataattt
     4861 gtcagcatcc tcaacatcaa atggcttact cactttagca gaaaagccaa tccctttccc
     4921 catcgcaatt ttttctattc catcgtcatc gatcaataca gcattatgat tcagcttttt
     4981 catcacatac accgttaatt cacctcctct atatgataaa acaaaacaac ttgcagactc
     5041 cttcatacat atttttgtct ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa
     5101 ccctttctta ggtcgtgcct aattgaatag tagcacccta cataatttac aaaaatagtt
     5161 taacacataa taaaagcgtt atcaat
//
LOCUS       DARDHD010000160         5185 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000160 DARDHD010000000
VERSION     DARDHD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.163"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1279..1944)
                     /locus_tag="SDA06_002308"
     CDS             complement(1279..1944)
                     /locus_tag="SDA06_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229563.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2124..4106
                     /locus_tag="SDA06_002309"
     CDS             2124..4106
                     /locus_tag="SDA06_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG4229564.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4110..4748
                     /locus_tag="SDA06_002310"
     CDS             4110..4748
                     /locus_tag="SDA06_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229565.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4776..>5185
                     /locus_tag="SDA06_002311"
     CDS             4776..>5185
                     /locus_tag="SDA06_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229566.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWY"
BASE COUNT         1713 a          791 c          946 g         1735 t
ORIGIN      
        1 caataaaaaa tgatttagaa tatcaaaata acaaaaaaga ggactacctg tattcgtcct
       61 cttttttgac cagattttga ccagtctaat ttacattcta atatattcat atgagctaat
      121 tttttgatta aatggtaagt ttttccttgt ttgttttagt ttaaactagt gtgtaattta
      181 aaaatctata tggaagggtc tgtggggtaa tacttaaata aaggctagga acctgataac
      241 atcaaggttc ctagcctttt tataataaaa tgaagcgaaa ataatttctt ttaaagagcc
      301 gagattatca gctatataag agtgaatttg atgcaaaaaa cagagccgaa acagtcttgg
      361 tgttcgtagc tctgcttata tgtttcgaat ttaagacaaa gagttggcaa aagatgttga
      421 taaggaagat aagataactg tgttatataa actaggtaac taactgtaac aagctaattt
      481 taagagattc ttattagaag acgtttagga atataactaa gaaaatgatt atacaggtaa
      541 ttgtcatttc aataatgttt tctaattttc gcgtctttgt tttaagaaaa aggccagaat
      601 cagttggaaa aaagttcgta cgatagtgaa gaggggcaaa atttcctact aatattccca
      661 ctaacaagag ataaaacggc caggttggtg tgattcctct ataaaaaaat gcccatgcta
      721 ccgaaatccc atagattaac aactgaacaa tttttacaat gaaaaaattg ttctgccatt
      781 actcaaagtc tcttcctcct tgtatcgttt atttgtttca ttttatcatt atgataggcg
      841 cttgaacaat ccattttgta ttttattgag tactcttgga taaactacca gcgattatca
      901 tatttattta tgttactgta agccatcata taaaagtaaa tgagcagatc atagaaatat
      961 aaaaaagaac taaaattgat ctaaattatg tgcagcttga ctacgtacca atctatatta
     1021 cttactagat tggtcgttaa tctattcgga tcgaatgagc aaaatatttt tgtgaatgtc
     1081 catccttgat tatgattcca aaaagaatca ttgggaggct gtggcgtaaa tattatacta
     1141 taagccagaa atacctatca ttctgaactt ttttatttag gaactttcat ctcaaggctt
     1201 tttcaattaa tttgtacccc tgttttttgg ataagatcat gtgcattgat tttttctatc
     1261 tctacgttca tctcctactt aatgcctatc tggctcctga gggcttcttt ttaaggcgtc
     1321 gtcatagtct tggctgagga tttcttggta atctgaggga atggcttccc aagtgagcag
     1381 gcgaacgttc atgacgtgtt ttcctttgtg tttgattaca gcatggtttt tctctttttc
     1441 tacctctcca gcgtgaaaag acagccatgt accatggcca aagatatcat tacataccca
     1501 acgttcgatg gaattgtcca cgtatcctac agggagtgtg gtatagccga tttgtggttt
     1561 caaaaaagga ttgaatcgat cgatcgtttc atttttgtat tgataattga taagaaaaag
     1621 atagaccaaa caaaagacga gcagaaagaa agatagtgtc tttttgagtg agaagggttg
     1681 ttttgatgaa taagatttct tgttaacaag aggttcatct ggcaatgttg gttctattga
     1741 ttcggtagct gattcaatag aaggaaaatc aatcggcaga cctaaagtat gatgtaaaag
     1801 tgacagcatt tcaaggtctg gcattgtacg agaattttcc caactagaaa tcgttttatc
     1861 ggaaacacca attttttcgc ctaattcttt ttgcgtaagc ttacaatttc tgcggtaagt
     1921 tcgtattttt tctccggtgt tcatcttctt ctcctttcga ttctacagta tatagattta
     1981 ttctacatga cctatacact tgatagatac gtttttttga tgaaaaacgg tatgatttta
     2041 tcaaagaatc atttaagtaa gggagagtgg tgataatcaa aaaaaagttc acagttttac
     2101 tcacaatcat agtgaccggt atcatgagtg gttttgcctt ggttagtata aataaacagg
     2161 tactctatcg gacaaatgaa tttttccatc aaacagatac ttatactccg attcgattgc
     2221 cacaacattt tattgggcaa gaagaatatg agcagctaat catggtattt gaaaatgtct
     2281 catcagaaac tgagctttct tatatgaaaa aaacagtaaa tgaatccaat gaagtagata
     2341 catcttggaa cttcaagata cagccaaagc ttgaaatgac attgtattat caaggggatg
     2401 tcaaatcttc tcacgagaag gtaccctatt ctaatacctc ctttcgcgta acgtccattg
     2461 aagaatcgat caaaataaaa gattttgaag gtgaattttt cttgatgacg agagaaaaag
     2521 aagcctatga acggttcatt tcactattca ctgatcgatt caatcaagca tttcacactg
     2581 attatgccac taatattttt gaagactttg aaaatagcta ttcgtattcg gttatttcaa
     2641 atacagacgt atataattat cgagtgtatt taactattgg attgattctt ttattccttt
     2701 tgttatcatc ctttttctta ttgatgaata aagaaatcag tttattgttg aataatgact
     2761 tttcattaca tcaagtattg tggtgtttat tggggaaaaa gaaaatcatt attacattca
     2821 taattgcagg aatcattggt ggttttattt ttatgtatta taaggagcaa tttctttatt
     2881 tgttccagat gtacgttttc ttgttcagcg gaatatatgg tatctctttg atttctcttt
     2941 tattagttga aaaaatcgct tcgttaagag aagcgttcaa ctatatccaa tacattcttt
     3001 ttacatttgg attgttgttt tctcttcttt tgaccgttgc caatgtcgaa ttagcaacta
     3061 tcgttttttc ttcttcacag ttagctgttt cccatgaacc accaaaagaa atcgctggtg
     3121 aggactatca tgtattttat ccagtaacca tcgggaaaaa ccacgtggat tttatttatt
     3181 ccaatcgttt tgcatcagca gcagatcagg aattatatga aactttgaat aaaaacggaa
     3241 gtatattagt caatgtatca gattacttga atgaagaaaa tgaacagttt ggcagaggga
     3301 taaaaataaa tctgaattat cttaagaaat ttcctttgat tgatgtctct ggaaggttga
     3361 tccaaataac cgaaaaagaa acgcaccctg ttatattgat ccctgaacga tatagacata
     3421 cagatttaaa aaatgacctg gcgcaaataa cagaatatta tcaagaaatc agtgaaaaag
     3481 aaccgaaatt tctatttata aaaaacaatc aacccattta tacatttatt ccttctatcc
     3541 cctggatcga acattatccg gttgttgaaa tactgacatt gaaaaacagt atgtatttgg
     3601 aaagaaatat tctcagcgga gaaatctatc caccgttaaa gatcaagata ggctctttgt
     3661 cgaaagaaag gttatttgag ctgattgaag aaagtcagtt acgtgataac ttgcagttat
     3721 catttccgta tactcagact gaagagatag ctgtcaaagt gttaagcaga tcatttcatt
     3781 acttgataca gatattttta ctcactttct tttctttctt cttgttgagc tatgtcatgt
     3841 tatgcatata ttttgtctac aattgtcgga aaatcgctat ctttcgttca agtggttatt
     3901 cgctctttga aacgtataaa gatttcttca tgatgaacct catcaaatgg ggaacgacaa
     3961 gtgtaatctt cctttttctt atagaaagag agcctaaata tctttttaat atttttttct
     4021 ttagcttcat tgattttatt ctttcagttg tttttatttt gtctacagaa aagaaaagtc
     4081 agttattgct gatgaatgga ggttaacaat tggagccgtt gatagtgata aaaggtgcga
     4141 aaaaaatcat tcgaaacaaa acgattcttc atttggatcg tttcactgcg gatgctgggg
     4201 atcttgtgac tgttgttgga gaaagtggag caggtaaaag tacgttattg aatatattag
     4261 gattgaatga tcagttcagt tcaggacaat tctatttgtt cgatcaagag acgaaacaat
     4321 taactgggag agagaagcgc ttgataaaaa gaaataccat atcttttctt tttcaagatt
     4381 ttgggttgat tgaatatgaa acaatcgatt ttaatttaga tattggattg aagttttcga
     4441 atttaaatgt ttcagataag cgaacagcaa agtatgaggc attgaaaaaa gtaaatataa
     4501 acaagaagct aagtacgcct atctacacat tatctggtgg tgagaagcaa agaatcgcct
     4561 gtgcgagagt cttattgaag aatagcaaaa tcattttagc cgatgaaccg actgggtcat
     4621 tggataaaag aaataaggat aaagtagttg aaatcttatc gcaacaagct tcccaaggta
     4681 cgttagttat cgtagtcacg catgatccat atctgaccaa aataggaaac aagaagatca
     4741 tgctttaaaa atcaaaaagg aaaggatgtc gttttatgaa aaaagcagtt attatttgtg
     4801 cactagggat aggttttgtt ggaggaaacg ctatgtcatc tgaagtggaa gcaaattcag
     4861 ttcacggtga aatctattac aaatctttaa agccaggaga gattcatttt gatgctaaag
     4921 gtaatattat taagtcaaat ttaccagtag accgggaaat ttctagaaca gcaattaatc
     4981 atagctctgg aagatgggtt tattatagtg atacaacttg tacgagaaaa acaggtcatt
     5041 ctaaccacta tagtcgtgtc tataatcgtc acggtgcaaa agcgaaagtt ggaaactcta
     5101 ttcgatcagc aaatgcaaca aaaggagtat gggcctatgc aagtgcgaca ggaaatagaa
     5161 ctgacacatt ctcatgttgg tacaa
//
LOCUS       DARDHD010000161         5169 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000161 DARDHD010000000
VERSION     DARDHD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.164"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SDA06_002312"
     CDS             <1..153
                     /locus_tag="SDA06_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229567.1"
                     /translation="ENFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHI
                     YMKKTL"
     gene            complement(231..764)
                     /locus_tag="SDA06_002313"
     CDS             complement(231..764)
                     /locus_tag="SDA06_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG4229568.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(858..2036)
                     /gene="mutY"
                     /locus_tag="SDA06_002314"
     CDS             complement(858..2036)
                     /gene="mutY"
                     /locus_tag="SDA06_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG4229569.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2029..2826)
                     /gene="recX"
                     /locus_tag="SDA06_002315"
     CDS             complement(2029..2826)
                     /gene="recX"
                     /locus_tag="SDA06_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG4229570.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2933..4315
                     /gene="rlmD"
                     /locus_tag="SDA06_002316"
     CDS             2933..4315
                     /gene="rlmD"
                     /locus_tag="SDA06_002316"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4229571.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4489..5067)
                     /locus_tag="SDA06_002317"
     CDS             complement(4489..5067)
                     /locus_tag="SDA06_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG4229572.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1494 a         1053 c          927 g         1695 t
ORIGIN      
        1 gaaaactttg ctaagaatca aggctataat acgattttat taggggcaca gtccacagct
       61 gaaacttttt acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc
      121 atgcctcata tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac
      181 agcaagcttt tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc
      241 gacaattgct tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct
      301 ccattattga tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg
      361 ttcgggtaat tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc
      421 tcgccgtcag gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg
      481 tccagcaaaa aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt
      541 gctattacat tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc
      601 catcggcgtc catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt
      661 tcgcttgttt ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg
      721 taactttgga tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa
      781 gcagcatttt gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca
      841 ataaaaatga gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct
      901 accagtttca tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac
      961 ttcgaagttt tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa
     1021 aggagaacat gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc
     1081 tgccaaacaa agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg
     1141 tcatcaaaca gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc
     1201 tcttgtgtca cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct
     1261 ctttcttcaa aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg
     1321 tcttttggct ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta
     1381 gctaaacaaa aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata
     1441 gctgaaccta gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt
     1501 ttccgcatcg cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg
     1561 caaaacaatc tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca
     1621 aatgcgatac ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt
     1681 tcttcaggcg tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct
     1741 tggatgttcc gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct
     1801 tctggagcat ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa
     1861 tcgatcactg tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa
     1921 ggatcacgat tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac
     1981 tgatcttgaa attcttttgt ttctttatct gtccattttt cccattcttt attcactttc
     2041 ctcttccttt tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa
     2101 gctttgcttt acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag
     2161 tttatctcct tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag
     2221 atcagctatc gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg
     2281 gatttttctc aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc
     2341 tgtctgatgt gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat
     2401 gatttcaggt ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc
     2461 acttaagcgc atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc
     2521 caattctttt aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt
     2581 taagtatgtt cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag
     2641 gtgaaaaccg aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa
     2701 ttcccgtcct tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc
     2761 tccttcagat aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt
     2821 taacataaac atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat
     2881 caggtattat atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga
     2941 aacaattgtc aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg
     3001 tgaaggaatc ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga
     3061 agaagtacta gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa
     3121 agtcaaaaaa acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg
     3181 tggctgtcaa ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct
     3241 tttgcaagca ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac
     3301 gatcgggatg gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa
     3361 taagcagact caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc
     3421 tataacagac tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca
     3481 actattgaat aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg
     3541 cacaatcatg gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg
     3601 cagtaaaaaa ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga
     3661 agttgtttca atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga
     3721 aacgatccac ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga
     3781 tctttctcct cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga
     3841 aggaatcaaa gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt
     3901 tgggacgatc ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt
     3961 tcctcaagca atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg
     4021 atatgaagtc gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc
     4081 tgatggtatc atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt
     4141 attgaaatac cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag
     4201 agatctggtt aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt
     4261 tcctcagact gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa
     4321 agaggttgtg aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct
     4381 cttcatgttt tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat
     4441 ttaaaaaaag ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt
     4501 cacgtctatc acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat
     4561 cacattatat ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg
     4621 aacagttgct ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg
     4681 attaggattc caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc
     4741 tgttaaattc caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt
     4801 agatgtctgt tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc
     4861 aatgaatgta tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt
     4921 ttgatttaag tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat
     4981 ataaccatgc gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat
     5041 aaacaataac gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt
     5101 aaacgcttta ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa
     5161 aggtgaaaa
//
LOCUS       DARDHD010000162         5086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000162 DARDHD010000000
VERSION     DARDHD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.165"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..149)
                     /locus_tag="SDA06_002318"
     CDS             complement(<1..149)
                     /locus_tag="SDA06_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG4229573.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFS"
     gene            307..570
                     /locus_tag="SDA06_002319"
     CDS             307..570
                     /locus_tag="SDA06_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229574.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            594..782
                     /locus_tag="SDA06_002320"
     CDS             594..782
                     /locus_tag="SDA06_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229575.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(865..1050)
                     /locus_tag="SDA06_002321"
     CDS             complement(865..1050)
                     /locus_tag="SDA06_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229576.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1265..1636
                     /locus_tag="SDA06_002322"
     CDS             1265..1636
                     /locus_tag="SDA06_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4229577.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1786..1858)
                     /locus_tag="SDA06_002323"
     tRNA            complement(1786..1858)
                     /locus_tag="SDA06_002323"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Gln,seq:ctg)
     gene            complement(1904..2332)
                     /locus_tag="SDA06_002324"
     CDS             complement(1904..2332)
                     /locus_tag="SDA06_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229578.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2392..3150)
                     /locus_tag="SDA06_002325"
     CDS             complement(2392..3150)
                     /locus_tag="SDA06_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG4229579.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3445..3642
                     /locus_tag="SDA06_002326"
     CDS             3445..3642
                     /locus_tag="SDA06_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229580.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3748..4230
                     /locus_tag="SDA06_002327"
     CDS             3748..4230
                     /locus_tag="SDA06_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229581.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4288..4884)
                     /locus_tag="SDA06_002328"
     CDS             complement(4288..4884)
                     /locus_tag="SDA06_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4229582.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1650 a          789 c          978 g         1669 t
ORIGIN      
        1 aaagaaaaaa tttgataagt aaaatataca gcaggtaaag aggccagtaa aactgaaaac
       61 ttcttttttg tccattttat gtaatagaaa agaatgctta tcataataaa aaaaggaata
      121 taaaaaacaa attgaccgat cgcgatcata ctattgcctc cgataaataa agtccatgat
      181 acaaaataac actttgtgcg aaatggcgca agcagttttg tggatttatc cggtaaaatc
      241 tgtatcgatt ttgaaattag aggagaaact tgctataatc aaagtgaaag aaggagtggg
      301 attttcatgg atcatgtagt gttagaaatc gatttgcatt tgatcaataa tattcgggtg
      361 atttactatg ttgtaagtaa cagtgtcgaa caacgtgtac tgacgaataa aataaatggt
      421 attttagcaa agaaagacgt tcaccgattc aataatggtg aaggaagtta ttatagtatc
      481 ccagtagaaa aaattattta tacgacagtc aaagtcagag aagatctaga taccaaaaaa
      541 gcatatgaac ctatttttac aacttattga ttgacagcag atggaggaaa aaaatgaatc
      601 catatgaaca acttattcaa gatttgatcg atggaaaaat cgaaaagatt gatgtaaaaa
      661 gagaagaatt aatggctttt cgagaagcgt ggctgaagtt ggagaatcgt aaatatgtgg
      721 tgggaaaagc ctcactgaac ggcaatgtga cttatcatta cgatccgaca cgtttatttt
      781 agtatgaagt attcagaaaa gaaccaaagg aggacgatgc agcagcattg tcctcctttg
      841 attttacaaa agtgaaagtt ttacttattt gaatagattt ttaaatttgt ttttgacact
      901 gtcgatattt acatctttta aaagattttg ggctttctct ttataaggtc ctaattgttc
      961 tttatgttct tcgataaatc gttttgcgcc atcgaaattg ccgttcttaa tcatttcctt
     1021 gactttttga aataattctt cttgattcat tttgaacaca tcctttgcta tcaacatgat
     1081 tttttacatg gacaaaacga gaaaaactga tttatccaca cgtcaataat atcaaataac
     1141 gatcattttt actaaaaaaa cactcgattc tcgcttctaa agtaggagaa aaatctccaa
     1201 cttgggacgg atggaatttc tttaactgac ccatatactg aatatataac aaggaggtag
     1261 aaagatgaaa gtgattaaat atttgattgg actggcggcg attagtggta ttttgctatt
     1321 tggactgaaa tattatactg aaggaagtta tggagaaatc cctggtatac ttgatcagct
     1381 aaatccatta gttgaaaaag gagaggtcta tgtaaagaca caaaaaccag aggaagtaaa
     1441 tgaatatggt acagcaaggt atgtgcagaa agctgctgat gccaatggga aagaacgaac
     1501 ggttgaattt aatggtcttt ctgtgttaaa agaaaatcat tatttgaaaa ttagcaataa
     1561 aggagcacac atagaaacgt atgaggaagt tcctagagaa aaagttccgg agaaagcatt
     1621 agcaattatt gggtaaatca gtttttgtat taagaatacg cccgatttta ttattcataa
     1681 tttaattatg taaactaaat acaagtagta aaaataatac aataatcccc ctgcaaaaag
     1741 attgttcttt tacagggggt tcattttttc agcaagcaca tcaaattggg atggcagggt
     1801 tcgaacctac aattgcttga ttcagagtca agtgccttac cagtttggct acatcccatt
     1861 tattcaacaa aataatcata gcactttcct aaaaaaacgc aagttatttt tcttgatcgg
     1921 atagttgttg ctttactcgt aaaaggcggc gtactagttt cttttctaat ccagcaggaa
     1981 gggcaatcag ttctgctact gacaagacgg tgtccaataa ctgatattct ttgacaggtt
     2041 cattcataat atgatagatg tttgctaaac gttcaatata ttcaaagcga gtatactctg
     2101 ttcgagcaat tagaaatttc aataaatcgg tggctttctc gtactcttga tctgcgtaat
     2161 aaacaagaga ttgacgatac aatttgattt ccagttcatt gagggtagca ggtctgcgac
     2221 gctcgttcca ctcattaata gagactttac cttgaacatt tcccttgcag tcacggacaa
     2281 aaataatcgg aatttcattt ggatccattt ttttatgtgt gaatttttcc attatctacg
     2341 ctcccttcaa aggtttggta tcttgagtat aagggatttt tgtttgaatt actagctttt
     2401 ctccaaagtt agcatctgtc cacccattgc agtgaaaatc gccgaaaaat cgttgatcgg
     2461 tactgctcga ttttttacac catagggatc gttgacataa atattttctt tgtcatatcc
     2521 agtgactaca caagcatgaa caagaggagt gactcttact tctccattat ttgtggtcca
     2581 agtcataaaa tcagtttcat ctgggacttc aaaattgata gtcacagaag tccagacagg
     2641 atttccttta gatagtgctt ttttgatatc atcgagttga gcatcggttt ttggaaaagt
     2701 gtgataatcc gtttggacga tttgctgagc aagggaggca actgggggaa catagacgac
     2761 catagcccag tctccgccac tgatatcacc aacaaatcct tcattaggat cgccatgagt
     2821 cccatctgca ttgaaaacag ggacgtaatc caattgctca gctaactggt ttttagttgt
     2881 ttcataacca taaaaagata aaagcataga aagcgaagtg acttcacagc cattttctaa
     2941 agaaggagaa tcaaactggc tctctaatgg aacatctagt aaaacttctg ctttctcttt
     3001 tgtctttttc gttttttgtg ttgaactttt ttgtgcagtg ttcattgtat gttctggaga
     3061 atggatatgt aggtaataac ttgtaccgct taataaaaga gcagctgtgc cccctagaaa
     3121 caatagtttt ttgaacgttt ttttcttcat ttgaaatctc ccttttcagc tgtttatctt
     3181 tcctattata aatgtatttt tattggaaat ggaagtatta tattttaata tgtaggaaat
     3241 aattagtttt taaggaatat gttttaattt tttgaaaaaa attcttctaa atacaacagc
     3301 tgttttaatt gtgaaaacga aaacagtacg ctataatgcc tgtaaggttg atttttaact
     3361 gtaaataaaa atcaatgagt taaatctcca aagaaatagc agtggtaatg tggagaaaag
     3421 ttccagcagt ttaggaggat tttgatgacg gaagaaatga ttaatttagg agaacaatat
     3481 gcatgcaaac caattggttt tacaaaaacg gtcattggtg aagttgtatc aaaaatgaca
     3541 aactgcgccg tggtcaaagt tgcccaatgt gctgcagagg accaagaatt attagatgaa
     3601 aaagcaagca tggttgttgc aaaatatgat acatttgaat aaagaaaaca attggacgtg
     3661 cggcgtgctg caagtccaat ttctttgaga aggtaaatca gtattctttt cctattttct
     3721 ggagaagaaa aagcgaggcg agttgtcatg gtaacattct ttacggttct attgttttta
     3781 ttcgttgttg tgatgttttt atttctttgg ctggttcgaa aagaaattat ttatcggact
     3841 gtgcgaaatc gatgggtcta cttggtgata cccttcttag tgtcactagt catttggtat
     3901 acactcatca gtcatcctac agcagatgaa ttggcaaaag ggatcttgtc agcaatgatt
     3961 tttattagtt ttttgttgga cagtagagga atcacagaag aaggacttgt tttgaatagc
     4021 tttgacaaaa aaggtgttcc tttttctgag atcaataaaa ttgtattata tcagccaaaa
     4081 ggttctaaaa tcgtaaaaat gaattttttc cgaaacggat ggcgaggacc gatgctgaaa
     4141 ttttctgcat cattagaaga attagttcca tttttgtccc aacgcttaaa cgatgaggca
     4201 gagattgata ttatgattga tccagaataa aaacagaggt tgcgacaaga tgtttgtcac
     4261 aacctctgtt ttttatttca aataaaatta tccaaatacg cggaagcgat cggcatcgtt
     4321 catgtattct ttttccccag ccggtgtttc tggtgaaccg aatactaatt gcgaacgtag
     4381 tttccagttt gaagggatat tccattcttt tgcaactgct tcgtcaataa ctgggttgta
     4441 atgttgcaag tttgcgccca atcctttatc aactaatgct acccaagtat tagctgttgc
     4501 gattccgttt gattgttctg accaatctgg gaagttgtct gcatataatt caaattgttc
     4561 ttgtagacct tttaccacgt cagtatcttt gaagaataat actgtaccat aaccattttt
     4621 gaaaccagct aatttgtttt gtgtatttgg gaaagcttct gctggagtca atgggcgaag
     4681 tgcttcttca gtgatttccc aaagtttttc atgtgcatct ccgaaaagga taactgcgcg
     4741 tgttgattga gcattgaaag cagttgggct ttctttaatt gcttctttga tcaaagttgt
     4801 taattcttca ttagaaagtg aaacattacg tcctagatga tagattgaac ggcggttttt
     4861 caaagtgtca gtaaaagttg tcataataaa atttctcctt tgatttgcgt tttgcatttt
     4921 tttaattgat tacgaaatga agtatagcac cttacaaaaa gtaagtaaac agatatgctc
     4981 acaaaaaata agcgaagttc aaaaaacaat tttaataaga aaaatcgtta tgtaaggatt
     5041 ttcttagtta tcgctttcat tgttgtgttt taggaatttt tgctta
//
LOCUS       DARDHD010000163         5061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000163 DARDHD010000000
VERSION     DARDHD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.166"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..660)
                     /locus_tag="SDA06_002329"
     CDS             complement(205..660)
                     /locus_tag="SDA06_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229583.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="SDA06_002330"
     CDS             complement(1063..1236)
                     /locus_tag="SDA06_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229584.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="SDA06_002331"
     CDS             complement(1917..2456)
                     /locus_tag="SDA06_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4229585.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="SDA06_002332"
     CDS             complement(2472..3395)
                     /locus_tag="SDA06_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229586.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="SDA06_002333"
     CDS             complement(4523..4678)
                     /locus_tag="SDA06_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229587.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="SDA06_002334"
     CDS             complement(4697..>5061)
                     /locus_tag="SDA06_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4229588.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DARDHD010000164         4938 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000164 DARDHD010000000
VERSION     DARDHD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.167"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            385..1896
                     /gene="gadC"
                     /locus_tag="SDA06_002335"
     CDS             385..1896
                     /gene="gadC"
                     /locus_tag="SDA06_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG4229589.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1909..3309
                     /locus_tag="SDA06_002336"
     CDS             1909..3309
                     /locus_tag="SDA06_002336"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG4229590.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3474..4914)
                     /locus_tag="SDA06_002337"
                     /pseudo
     CDS             complement(3474..4914)
                     /locus_tag="SDA06_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1556 a          807 c          934 g         1641 t
ORIGIN      
        1 acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc tcaagttatg
       61 tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt tttggactta
      121 tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact aaagatatta
      181 atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa aatgaaaata
      241 aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta gaaaagaggt
      301 agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg aatgacaaat
      361 taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt cggttttttt
      421 gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc aacatcacaa
      481 ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc agttgcttta
      541 tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt cagttgggtt
      601 agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg gtttcaaatt
      661 acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta tgtaattaat
      721 tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt cattttctgg
      781 gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc taaagcagga
      841 tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc agcctacttt
      901 atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc tgacttttct
      961 cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg cgtagaagct
     1021 tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt ggcaatgttt
     1081 ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt agcagcggtt
     1141 attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga aacgcttatt
     1201 ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat gattgcctgt
     1261 ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat gtttgcagct
     1321 gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga cgttccagtt
     1381 ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt aacttttggt
     1441 ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt catttatctc
     1501 gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa aaaagactta
     1561 aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg tggaattggt
     1621 tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc aattgcagct
     1681 agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac tgttctttta
     1741 ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga ggaacctact
     1801 cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg agagcaccac
     1861 gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat gttatacgga
     1921 aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc aagtgaggat
     1981 gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat tgcttatcaa
     2041 ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc tactttttgt
     2101 caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga aaaaaatgca
     2161 attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt aaatatgatt
     2221 gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac gatcggctct
     2281 tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa acgtgctgaa
     2341 aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc tggttaccaa
     2401 gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt cccaatggat
     2461 gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga gtacacaatt
     2521 ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat caagggtctg
     2581 aatgatttag ttgaagctca caataaacaa actgactata aagtatacat tcatgttgac
     2641 gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg ggattttcaa
     2701 ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt atatccaggt
     2761 gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt atttaaagtt
     2821 agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag tgcagcacaa
     2881 ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg tgatattcac
     2941 caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac tggtattttt
     3001 gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa agaagatccc
     3061 aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg atggcaagtc
     3121 ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt agttgttcga
     3181 gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca agcaattgaa
     3241 gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac gtacggcttt
     3301 actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa aaggagttgt
     3361 gattaccaca cagctcctta aatctatttg aataaatata tgatattcat ttgttacact
     3421 ctcaatctta gctgttgttc tattcatcat tactcttatt gtaagtagtt cagtcaaatt
     3481 tgcttgttca attgatcgat aacagtatga attgtttgaa aagtaatcgg atatataatt
     3541 gttttctcac tacctaagcc ttcttggtat aaagaactat cgcaaagtat aatccgctga
     3601 gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt tacaaatgat attactaata
     3661 gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat agtataaata cctaaagtct
     3721 gacggagcta ctatgaacac ttcaattgga gatttccgtt tatgctcatt taaaagactt
     3781 aaactaatgg ttagtttacg aatagaattc tcattccaac gcagaaataa attgttttgt
     3841 tttcgccatt gttcaagaat ttcatgaact tctaagtaca aagcgcgttg ctcttcattt
     3901 aacaagtaag tcttatcagg ttgaaatatc tgaatatcag cccaaagtga gcggataaac
     3961 gggagaagtg ctttatcaaa aagtatctga ttttcttcag atatattcag agataagata
     4021 agtttttgac tgagctcaat caccaaaggg ttatctgcta catggttttg ataaacaacg
     4081 ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat aatttctaga gttgaataga
     4141 aagtagatat aaaggcattc atcttcgtca tgacataaag agaaagaaag ttgctctaat
     4201 cctttctgta ttagctgaaa caagggagta tgtcgggcaa ctcttttttc ttcctgacta
     4261 aaatatattg ggttagtctt agatctctgc aaaccgatag cgatacctaa taaaataaat
     4321 tgtttttcaa ctgtagggta acttcttgaa aaaaaatgtt tttctacata atcaatcaat
     4381 tgttgaccgc tttgaataat tgatttattt agctggatca actctgtccg atttgtctca
     4441 tagattatcg ttagtaacaa acttcgataa tccttttcag gaatttcaaa atggccatca
     4501 gcatttaagt actgcatttg tttaaaaaaa ttaatcaact catttcgtaa gttgtaaact
     4561 ttgcttaacg aaatgaaggt agtatcagct atattactcc aattgtgttc ccctgataaa
     4621 taatgtccta gtacattgaa aaagtcagag cgctgataga tgagttgagt gagtgccaac
     4681 ctcgaaaacg aagagtcgaa tgcgactgta aagctacttc tagatttctc cactgaaata
     4741 atttgctgat ggagtatgtc ggtaatgcat tccaagtcgt tagtgacaga cagtgagtgg
     4801 gcaaacgttc aattgatctg ctatttcgcg aatctgaatg gtcccttttt cgtgtaattg
     4861 ctcacaaatg taaacttgac gaaaaatact tttctccaga taggtttcca acatagtact
     4921 cctcctagtc attttata
//
LOCUS       DARDHD010000165         4909 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000165 DARDHD010000000
VERSION     DARDHD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.168"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            863..1774
                     /locus_tag="SDA06_002338"
     CDS             863..1774
                     /locus_tag="SDA06_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG4229591.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2261..2527
                     /locus_tag="SDA06_002339"
     CDS             2261..2527
                     /locus_tag="SDA06_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229592.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2599..2940
                     /locus_tag="SDA06_002340"
     CDS             2599..2940
                     /locus_tag="SDA06_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229593.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2995..3414
                     /gene="ssb"
                     /locus_tag="SDA06_002341"
     CDS             2995..3414
                     /gene="ssb"
                     /locus_tag="SDA06_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4229594.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3491..3787
                     /locus_tag="SDA06_002342"
     CDS             3491..3787
                     /locus_tag="SDA06_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229595.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3802..4233
                     /locus_tag="SDA06_002343"
     CDS             3802..4233
                     /locus_tag="SDA06_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4229596.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4415..4720
                     /locus_tag="SDA06_002344"
     CDS             4415..4720
                     /locus_tag="SDA06_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229597.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1805 a          756 c         1029 g         1319 t
ORIGIN      
        1 tttgaaacaa attcactcat tttttgagca aaaatgtttc aaaatttgaa caaaaaccaa
       61 ttgaaaattg aagcaaattt cactcgaatt gaatcgattt tgagctaaaa tcaatagaaa
      121 aaataaacgt attttgtttg aaatccgttt atttttttta cgtttcagta ttagtaagga
      181 ttatttgagg gttatttaga gattatttaa aggttatatg taataagtgg ggggagggct
      241 gtcggcgctt ggtatgaaag gcattctccg acttccaggt ttttgaccat tttctctcaa
      301 cgtggaaaaa ccgccactta tgagagaaaa tggtcaaaaa cctaagcggg aagtctccga
      361 ataacatacc aagcgccgac agccaacaga taaagaatac actactaaaa atagtagtag
      421 cataaaattt aaaagggaaa aaatcaaccc ctaccccagt cttggggtct caaaactaac
      481 tcccccctaa ccccctcttt cagcctcttt tgaaagaggg ggagaatcaa ccccacggcc
      541 aacgccccag tgttcttggg gctgtccaga acagaaactt gcgagagagt gggggtaaaa
      601 caagtgtaga ggaaacttga agacttgttc gaacagaaac ttgcgagaga gccggagtaa
      661 aacaagtgta gaggaaactt gaagacttgt tcgaacagaa acttgcgaga gagttggagt
      721 aaaacaagtg tagaggaaac ttgaagactt gttcgaacac aaacttacaa gagaggggag
      781 taaagcagtt gcagaggaat caggaagatt tgttcgaaca gaaacttgca agaaagcatt
      841 attaaattga aggtgatgaa atatgaagta tttattgcat ggctatttta ttgattattc
      901 tactcgcaat agtagtactt tagcgattct atgtttgctt catgaattga gagtggttac
      961 tagaaaacag ctatacaaac tattgaattt tgacagatca attagtaaac aaggagtaac
     1021 gaatgcttta gtgagattat atgaaaaaca gttaatcgat cgaaaaaaat atggaacaga
     1081 aatgtattat tatttgacga aagagggaca tcaaagcata ggtgggtatt atacgttgcc
     1141 gaaagttcct gaatataatt tgaatcacca tttacaaatt aacaattatt tgattaaagg
     1201 aatggcattg gcaaaagacc acccacattt aaaacggatt atttcagaaa gacgtaccgt
     1261 ctatgaaacg aaggatttaa atcctaagac aaaaggaaga aaattttttg tgccagattt
     1321 tatttttcgt tttcgagaca aagaaaatca tgagatcaat tggtcctttg aaattgagct
     1381 aacgttgaaa acacgtcgtc gctaccgtga agcgattttt ccgaaatata ttcgtgagtt
     1441 gcgaacacgt cctgaatcac atttaattta tgtgacacct agtggcgcaa ttcaagaaga
     1501 acttcatgcg tttaagcgta tttttttcaa gcgcgaaact gaaaaagatg cgagtcaaca
     1561 agaattgttt gagcggttgc atattttttc agctttagag tttgaagagg gattaaaagc
     1621 attattagag aatgatccag cagtcaattg gcggacagta gaacgagaaa aaatttatcc
     1681 gaatggcgct gttcaaagtc gtttgaattt atttgaagaa tcaactgaaa atgaggaaaa
     1741 accaaaagtg aagtttacgc ctgaagtatt gtaaaagagg gtgttcgctt cgctcttttt
     1801 ttgtgccagt ttgctggcac cgttcagacg caagcgtctg aaccggctcg tttttaggaa
     1861 ggactaactt gtttgattta tccgttgtca aagaagtgga cgttcgagag tccctaatcg
     1921 tatagcagtc attcggcgag tataacctaa aacataacct gccaattgcc aagggtaaaa
     1981 tgcacgctgc gcgcccttgg caatcgtcag aacatgtttt cgagccggtt atgccgaacg
     2041 accgctatac gaaaagggcc tctaaacaga agtccacttt gatccttgac aactgaaacc
     2101 caaaatcaaa caagcaggcc ttcgtctaaa aacgagccga atgaagacct tgggggttgc
     2161 acccccaaac ccccgtgccc ccgacacact accgaaaagg tgggggtggg tggcgaaaaa
     2221 atcaaataaa acattctatt caagaaagga agaaacaacc atgacaacta aaaacgagca
     2281 actgaaagaa caggcaagaa aagagttgca agaaagaggt ttgattattg aggggagctt
     2341 tgaaggagat tttgaaacct atattggttg ttatgcaaga ccgataaaca aacctacagc
     2401 cttagaccca atgaacgaac aagaagtaaa agaacaagaa aaacatgcag tgaatggttt
     2461 tccgcaagat ttcactgaat ggtatgagtg ggaaattaac aacgggaagc ttgaaaactt
     2521 cttataaaag tcagatgacg tgcaaagaga atgattctct ttgtacggta aaaatataaa
     2581 gaaagaagga aaaagaaaat ggcaaaaaac acaccttatc aagtaggaga cattttttat
     2641 agtagttggg gctacgaaca aacgaacgtg actttttggc aaattgtgaa gttaacagaa
     2701 aaaacagcat ggtttcagcc tattcaaaag aaaacggtcg agcattattc gtccatgagt
     2761 ggaaaagttg ttccaatgaa agacaagttt atagagtatc ctttagcaaa aactaaagac
     2821 agtattgtaa aaaaacgaat tgatccagaa acctatcctg atatgttatg tggttatcgt
     2881 agttgggaca cgtttcatag atataaaaat caagctgttt atgaaagtag ttattattaa
     2941 ggggatagtt tcccctttca aaaataataa atagaagaat ggagagaaaa aatcatgatg
     3001 aattcagtga atttagtagg acggttaaca aaagaagtgg atttaaaata cacaccaaaa
     3061 ggcaatgcga caggcacatt tattttagct gttaatcgaa attatacgaa tgcaaacggc
     3121 gagcgtgaag cagattatat ccgctgtgtc atttggcgga aagctgcgga aactttagca
     3181 aaatatactg gaaaaggaag tttgattggc attaatggac gtcttcaaac gaggagctat
     3241 cagaatcagc aaggacagac catgtatgtt acggaagttc tagtgacaga tttctatttg
     3301 ttagagagta aagaaatcaa tgagcaacgg tcaaaatcaa tggcgaataa tcaaaataat
     3361 tcttcaattc caggaaatga gattcaaatt tcagatagtt atttgccctt ttaagtgaaa
     3421 aataattttg ttctatacct gatttcttca ggtatagaac aaaaaataaa tagagatgag
     3481 gcgatttttt atgaaaatca ttgacagtta cgatcaacga accatgcaat atttgggctc
     3541 gtttgattat acagatgaaa atattattaa ttatgttgca agtttacctc cttatcaaag
     3601 cgttcgcttg gtagattttg caagtgatga attggtatta acaacgatgg gcaacttttt
     3661 agattatgtc ggagacaaaa aatggttgga aaaaattttg tctaaattga ttgcgaaaca
     3721 aacaggagaa gaacccataa atgcgattca attgttcaat cgatatgaac aagaaaacaa
     3781 tgattaaaga ggagagaaaa aatgaaaaga ttaaaacgaa gtatttttat tgtgctagca
     3841 ttgattgatt ttgttgggac aggttgtgaa gcaattgacg ttcaaaaagc tcccttatcg
     3901 gaaataaaac aagcaataac aaaacaagat gaagaacaac cgctaaatac acaagtagaa
     3961 atagtgaatc aaaataaacc gagtttttct gaagaagatt tttcgttaga aaatggaacc
     4021 aggcaatttt tttcagattt ggatcgttta aatcgggtag gtgtagcaag tgcacttttg
     4081 cataaaagca ggatgccaac agaagaacga gaccgattat acattaagcc aactggctgg
     4141 aaacaaaaga aaatgaagaa tggcgattat ctttataatc gttgtcattt gattggctat
     4201 caacgtgcga caagtttccc cataagtatg taatacgaaa cgggaggatt aatcttaaga
     4261 ttaatcacaa ctaacctatt tgacagttgg aatgaaagct atcatgtctg ctgaatcaac
     4321 tggtctgata ctcctacatg caataaagtc tactaggaag ttattgtatg aagctaggta
     4381 aaggcggcag aacaatttct aagtgtggaa tcatatgaag ttggatatgc cgggacgcta
     4441 taaattactc agggtgagac tgtttttaat taaactgacg aacttgcgaa tgtacgggtc
     4501 tataaccgac ttatattcga aagatataag attgcgaatg caaagaacat tatcgaaaag
     4561 taagaatgtt ggtcatgaaa ttcgagatgg aagactagcg gtgtatctgt caagagttgg
     4621 taaaacatgt ttgattgatt cagatgagag tgggaatgcg acaaaacgtt ttacagaaga
     4681 aatagagcaa ctagcagaac tgaatatacg atatttttaa atactttagc ggagaataaa
     4741 aaaatcctct acagaaaact acggagggca acatgtgcta ttgtttcaac aatacatttt
     4801 taatttttac cataacaaaa aagtacttga aaaaaaacag aaatatgtta aattaaaaat
     4861 gaaaaaatgg aagcggttac ttattctttt gaatctaaga taaaaattc
//
LOCUS       DARDHD010000166         4849 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000166 DARDHD010000000
VERSION     DARDHD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.169"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..290)
                     /locus_tag="SDA06_002345"
     CDS             complement(<1..290)
                     /locus_tag="SDA06_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4229598.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="SDA06_002346"
     CDS             complement(283..624)
                     /locus_tag="SDA06_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229599.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="SDA06_002347"
     CDS             complement(798..1523)
                     /locus_tag="SDA06_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG4229600.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="SDA06_002348"
     CDS             1576..2052
                     /locus_tag="SDA06_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229601.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="SDA06_002349"
     CDS             complement(2058..2240)
                     /locus_tag="SDA06_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229602.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="SDA06_002350"
     CDS             complement(2240..2539)
                     /locus_tag="SDA06_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229603.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="SDA06_002351"
     CDS             complement(2644..2847)
                     /locus_tag="SDA06_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229604.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="SDA06_002352"
     CDS             complement(2862..3608)
                     /locus_tag="SDA06_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG4229605.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="SDA06_002353"
     CDS             3686..3994
                     /locus_tag="SDA06_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229606.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="SDA06_002354"
     CDS             complement(3965..4222)
                     /locus_tag="SDA06_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG4229607.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="SDA06_002355"
     CDS             complement(4520..4660)
                     /locus_tag="SDA06_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229608.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1330 a          938 c          760 g         1821 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttg
//
LOCUS       DARDHD010000167         4796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000167 DARDHD010000000
VERSION     DARDHD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.170"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SDA06_002356"
     CDS             complement(<1..258)
                     /locus_tag="SDA06_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229609.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SDA06_002357"
     CDS             complement(274..924)
                     /locus_tag="SDA06_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229610.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SDA06_002358"
     CDS             complement(935..2107)
                     /locus_tag="SDA06_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG4229611.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SDA06_002359"
     CDS             complement(2126..2998)
                     /locus_tag="SDA06_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4229612.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SDA06_002360"
     CDS             complement(3020..3940)
                     /locus_tag="SDA06_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229613.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SDA06_002361"
     CDS             complement(3940..4209)
                     /locus_tag="SDA06_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229614.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SDA06_002362"
     CDS             complement(4206..4586)
                     /locus_tag="SDA06_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG4229615.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1254 a         1053 c          791 g         1698 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgc
//
LOCUS       DARDHD010000168         4791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000168 DARDHD010000000
VERSION     DARDHD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.171"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..1118
                     /locus_tag="SDA06_002363"
     CDS             72..1118
                     /locus_tag="SDA06_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4229616.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="SDA06_002364"
     CDS             1135..2358
                     /locus_tag="SDA06_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4229617.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="SDA06_002365"
     CDS             2447..3334
                     /locus_tag="SDA06_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG4229618.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="SDA06_002366"
     CDS             3420..3650
                     /locus_tag="SDA06_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG4229619.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="SDA06_002367"
     CDS             complement(3707..4672)
                     /locus_tag="SDA06_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4229620.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1622 a          794 c          973 g         1402 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga g
//
LOCUS       DARDHD010000169         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000169 DARDHD010000000
VERSION     DARDHD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.172"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SDA06_002368"
     CDS             564..989
                     /locus_tag="SDA06_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229621.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SDA06_002369"
     CDS             1019..1183
                     /locus_tag="SDA06_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229622.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SDA06_002370"
     CDS             2141..3118
                     /locus_tag="SDA06_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229623.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SDA06_002371"
     CDS             3128..3625
                     /locus_tag="SDA06_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229624.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SDA06_002372"
     CDS             4001..4282
                     /locus_tag="SDA06_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229625.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SDA06_002373"
     CDS             4284..4700
                     /locus_tag="SDA06_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229626.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARDHD010000170         4776 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000170 DARDHD010000000
VERSION     DARDHD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.173"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            255..1178
                     /gene="truB"
                     /locus_tag="SDA06_002374"
     CDS             255..1178
                     /gene="truB"
                     /locus_tag="SDA06_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG4229627.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1182..2123
                     /gene="ribF"
                     /locus_tag="SDA06_002375"
     CDS             1182..2123
                     /gene="ribF"
                     /locus_tag="SDA06_002375"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG4229628.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2475..3260
                     /locus_tag="SDA06_002376"
     CDS             2475..3260
                     /locus_tag="SDA06_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4229629.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3273..4124
                     /locus_tag="SDA06_002377"
     CDS             3273..4124
                     /locus_tag="SDA06_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG4229630.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4248..4700)
                     /locus_tag="SDA06_002378"
     CDS             complement(4248..4700)
                     /locus_tag="SDA06_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229631.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         1581 a          786 c         1033 g         1376 t
ORIGIN      
        1 tttgggaagt gaaaaccaaa ctatttacct gtaagccacc cataaatcca atcaaatggt
       61 gcaaagaaaa aatcttccat tctattccct cctttaaaaa agcataacat gatttctatt
      121 tacgtgagca tgaattttgt tacgttttta ttgccgaaaa ataaaaaccg ttcattatga
      181 tgtcaaattc gtcagtaatt gatgcaatgt gttataataa ttcagttaaa tcaaaaatga
      241 aatggagaaa agacatggac gggttattac ctttatggaa agaacgcgga atgacaagtc
      301 atgattgcgt attcaaatta agaaaaatct tacatacgaa aaaaatcgga catgggggta
      361 cgcttgaccc agacgtagac ggtgtgttgc ccatttgtat tggaaaagcc acaaaagtta
      421 tcgaatattt gacagattct gggaaaatct atgtaggcga gataactcta ggtttcagta
      481 caacgacgga agatcgatcc ggagaacggg tagaaacaaa accagtagat atgtctttat
      541 ctgaaaaaaa aatagatgaa gtgatggctt catttattgg tgagatcacc cagataccac
      601 caatgtactc agcggtaaaa gtcaacggta agcggttata tgaatatgcc cgcaataatg
      661 aaacagtgga gcgtcctgtt agaaaggctc agatttaccg ttttatccgg acaagtgaat
      721 tgacaagaga cgaagtgaca ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag
      781 gaacctatgt ccgcacatta gctgtagata caggcaagaa acttggttac cctgcgcaca
      841 tgtctgatct gacccgaaca gcaagtgccg gtatgtccca gaaggatgct ctgacattag
      901 aagaagtagc tcaagctgtg aaaaatggga caatcgatga ttgtttgctg ccaatagaaa
      961 aaggagtgga acggttcaaa cgagtagata ttactgatga agtatggcag aaagtaaaaa
     1021 acggtatgag acttgattat actgtatttg cgctcccaga aatgccgtca gaagaaattg
     1081 ctttgtttta taaaggaaaa gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca
     1141 aattaaaacc tagtaaagta ttgagaaatg aggaataacg aatgaatatc atcaagatca
     1201 gacatcctta ccaatcagaa caaattccag aagacgatgt cgttttagtg ctgggatttt
     1261 tcgacggcgt gcatatcgga caccagaaag tcataaagac aggtatggaa attgcacaaa
     1321 aagaaggatt gaaattagca ttgatgactt tcaatcagca tccatctatt gtctttaaaa
     1381 agatcgatcc tagcagcgtg aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg
     1441 ccgctttggg tatagattat ttatatgaag ttgattttac ttcttctttt gctcatttag
     1501 caccgcagga atttgttgat caatacatcg tagggttaca tgcaaaatat gctgtttccg
     1561 gttttgatta tacgtatgga ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg
     1621 ccaaaggaag attcaaaatc gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca
     1681 gctctacccg tattcgtgga ttactagaca gcggacaagt aaaagaagca gcaaaactat
     1741 taggttgcac atatgaggta gaaggggtag ttgtccatgg cgatgcacga ggcagactac
     1801 taggattccc tacagcaaat gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg
     1861 tttatgtgtc ggaaataaaa attggggaaa catggtatcc agccatgggt tcaattggac
     1921 ataatgatac attcggtgaa ggacgggagc tgactgtaga gttgtatatt ttggacttta
     1981 atcaagatat ctatggtgaa catgtcgcgg tacgctggaa tcaattttta agagatcaag
     2041 tgaaattttc tagtgtagag gaattgattg aacaactcaa ggaggatgaa caagcaacag
     2101 ctgaatattt ttctcagcaa taaaacatta tgcataaaat ctcttgttta tgtataacta
     2161 gcaattacaa taaaatgggt gataaataag cccaggaaag gggctgtgac aaaagtcttg
     2221 tcacagcctt tttttccaaa taaatggttt ttcagaagta gcttcttcgg aaataagccg
     2281 aaatttcttg aagctgacca cttctgtcac aacctctttc tttatagaaa taaaaggaca
     2341 ctagcaattc atgaaaacga ttgctagagc gtatcatttg ctttagacta tatatcttgg
     2401 tgtacactca ctttaaggcg gaaataaaag ttatccgtaa taacaggttt aggtatgaaa
     2461 aaggagtggg tactatgtac aaggaattag aaaacaaagt cgcagtagta actggtggct
     2521 caaaggggat tggaacagcg attgctcaac gattcgggaa agaaaaaatg aatgtagttg
     2581 tcaactataa ctcggataaa gaaggagcag aaaaagcagt caaagcaatc gaagaggcag
     2641 gcggaaaagc agtctctgta caagcagatg tcagttctga agaaggaata cagcgtttac
     2701 ttgatacggc tttagaaaat tttgggacac tggacgtttg ggttaataat gctgggatgg
     2761 aaaatcagca tccaacccat gaacttagcc ttgaagattg ggaaagagtg ttaaaagtca
     2821 atttgactgg cgtttttatc ggaacgaaag cggctttaaa ttactttaca gagaataata
     2881 aaaaaggaaa tatcatcaat atgtcttctg ttcatgaaca gatcccatgg ccaacgtttg
     2941 ctcattacgc tgcttcaaaa ggcggggtaa aattatttac cgaaacggta gcaatggagt
     3001 atgcaccgaa gggaatcaga atcaatagta taggccctgg tgctatccgt acacctatta
     3061 atgcggcaaa atttgatgat cctgaacaaa aagaactctt ggaaagtatg atcccactag
     3121 gacgcgtagg tgaaccaaaa gaaatagcgg cgacagctgc atggttggct tcagacgaat
     3181 ccagttatgt tacaggtatt acactatttg cagatggtgg gatgacttta tatccttctt
     3241 tccaaggtgg aaaaggataa gggtgaataa aaatgggttt attgattgca ttgatcccgg
     3301 cagttggctg ggggatccag ccactgattt tgaaaaagat tggtggcaga ccaacaaatg
     3361 agatattagg aactggtatt ggagcggtgc ttgtcggctt actcgttcaa ttattcgctt
     3421 ctccagctgg tatttcagtg actacatttt tgattagttt actttctggt gcattttggg
     3481 ttattggaca aacaggacag tatacatctt tcaacttgat tggtgtttct aaaacgatgc
     3541 caatttcgac tgcattacag ttagtcggaa cctcgttgat tggtgtcttc gcttttggag
     3601 aatggtctgg aagtatgggg aaaatcattg gagcagcagc aatcattctt cttgtatttg
     3661 gttctgcttt aacggcaatc agtgatgatc ctggaaaaaa aggcggtatc acaaaaggaa
     3721 ttagtctatt agcatcaaca agtattggtt attgggtata cagtgcattg cctaaactag
     3781 ttgatgcaag cggactttcc attttctttc cgcaaatgat cggtgtcttt ttaggggcag
     3841 tgatttatgt gattttccgt aaaccaaccg cattttctga agacaagagt tggaaagcaa
     3901 cgatcgttgg agccatcttt agtgtgtcag cattggctta tattttttca gcaaatgaaa
     3961 atggggttgc tactgcatat atcattacac agctaaacgt agttatctct acattaggcg
     4021 gtatgtacat cctgcatgaa aataagacac caaaagaaaa aaaattaacg attatcggat
     4081 tattgctgat cgtagtagga agcatggtca cggtctttat ttgaggataa agatggtaaa
     4141 atttcattta ttaaaaaaat atttgtttga accaaacagt aagtacttat aaaaaaatag
     4201 cgctagaatt tccaacatct ctggaatttc tagcgctttt taattgttta agatacaagg
     4261 gaaatatctg tctggatcaa taatttttca ttgatccatt gactaaagtt tacgacataa
     4321 ttgaagttac caccgattag tatctccgca tgattgtctt tataaagatc gtattgtaaa
     4381 agacggtctt ttttatttgc gtaaacatcg ttatggtact ggactaattg ttctcggaat
     4441 tgattgattg ctgtaaaatc acgatattga aggttgaaac gcatcgtgat tggtgcattt
     4501 agcatgaatc gctgcatttc ttttgctgct tcctctgctt gatctccgta taggaactca
     4561 aaaagattac gttcagaaaa tttccattgc ccgccaacct ttgttccttt tagttttcct
     4621 tcatgaagat aatttctgat agaacgtgta gtaacaccta attgttcggc taattcattt
     4681 actgagtaaa aatctttcaa tgttcaccct cctatgtgaa tggttctact gacttagttg
     4741 atatttgctt aatcacgaaa tgtagaaaga acgatt
//
LOCUS       DARDHD010000171         4746 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000171 DARDHD010000000
VERSION     DARDHD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.174"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SDA06_002379"
     CDS             complement(182..1144)
                     /locus_tag="SDA06_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229632.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SDA06_002380"
     CDS             complement(1146..2585)
                     /locus_tag="SDA06_002380"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG4229633.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SDA06_002381"
     CDS             2770..4716
                     /locus_tag="SDA06_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG4229634.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1509 a          810 c          785 g         1642 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aatttt
//
LOCUS       DARDHD010000172         4733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000172 DARDHD010000000
VERSION     DARDHD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.175"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            115..924
                     /locus_tag="SDA06_002382"
     CDS             115..924
                     /locus_tag="SDA06_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4229635.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            964..1461
                     /locus_tag="SDA06_002383"
     CDS             964..1461
                     /locus_tag="SDA06_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4229636.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1846..1919)
                     /locus_tag="SDA06_002384"
     tRNA            complement(1846..1919)
                     /locus_tag="SDA06_002384"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1883..1885),aa:Ile,seq:cat)
     gene            2106..2429
                     /locus_tag="SDA06_002385"
     CDS             2106..2429
                     /locus_tag="SDA06_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229637.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2593..2847
                     /locus_tag="SDA06_002386"
     CDS             2593..2847
                     /locus_tag="SDA06_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229638.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2917..3162
                     /locus_tag="SDA06_002387"
     CDS             2917..3162
                     /locus_tag="SDA06_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229639.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3238..3603
                     /locus_tag="SDA06_002388"
     CDS             3238..3603
                     /locus_tag="SDA06_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229640.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3664..4227)
                     /locus_tag="SDA06_002389"
     CDS             complement(3664..4227)
                     /locus_tag="SDA06_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229641.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1501 a          832 c          793 g         1607 t
ORIGIN      
        1 tgttttttga aaaaattcat aatgacaaag caagttagtt tcattttttt aaattctcat
       61 ttaatcttaa ggtaacagga aaatagatat gtaaaaagaa agtaggaaga taaaatgaaa
      121 ctagcagcga ttgatttaga cggtaccttg cttgatcgaa atggaaagat accagaagaa
      181 aatatccaag cacttcggaa atttgatgaa aatggcggaa tcgtaaccgt cgctaccgga
      241 aggaacagca tttctgctaa agatattttt tccgaattag gaatcggtgg ctatttgatt
      301 tcttccaatg gttcactgat tgctgaaatg aaggatggaa aaatggcgaa agttctcaga
      361 cgttcaaaaa tagatagcga tacactgaga aaagccttct tctatgctca agaagcgaaa
      421 atatccatca ttgctagccg agaaacccaa gatgatcagc tgacattcga tgaatcaaat
      481 ctagtcaaag atgaccctta ttatgaacat ttcaatttac agaaccattc ttttgatcaa
      541 attttaaata tgttggaaga tccttctctt agctatctta aattagcatt aacagataag
      601 agtgaagaaa aactacagaa gctacaaaga gatctgcaag cagatggtat ccaatcagta
      661 ttctctgatc cgcatttctt agaaatcacg cctacaggta tcactaaagc tcattcactt
      721 gtttttctgg cggattacct tggaatcgaa tcgagtgaca tcatggcttt tggcgatcaa
      781 gaaaacgaca ttgctatgtt gaattacagc gggttgagta ttgcgatggg gaatgcacag
      841 cgacaagtaa aagaaattgc aaaccaagta acactgacga atgaagaagc tggtgttgct
      901 tatattttaa aaaacttcta ttaaaaagaa taaaatgacg atatgaatgt aggaggaaag
      961 aaaatgttag ttattattga tatgcaaaat catattcttg atccaaacag cgaattttat
     1021 attccagagt cagatcaatt ggcagaacgt attgctcaaa gattagctaa agcacgccaa
     1081 aataatgaat atattctctt cacacgggat ataccagttg aactgaagga caaagaagaa
     1141 gaacgagaag atttgcaatt gatccctctt ctttctccac aagaaaatga acgagtgatt
     1201 aaaaagtatt actttacgat tccacctgaa actttagctg aaatccagga aactttattt
     1261 gaaagcaaag aagaacaaaa agagatcgaa gtagtcggga ttgaaacaaa cttgtgtgta
     1321 ctctctaatc tgataggcct gcagagtgct tttcctgaag cagacttttt tgttgatcca
     1381 atgttagtct ccagtagaaa acacggagaa tccgctttag agctgttgaa agatttcaac
     1441 gtctctatcg tcgaatcata aaaatatcta tcttcattta cgatatttaa cacacaataa
     1501 aaaaagcacg cagaactgtt ttagtcctac gtgctttttc tatcaaagta gattggcttt
     1561 tttatgacac agagtgaaac ggcaggctca cgacctcttt cttaaatggt gttcaatcag
     1621 ctgaccctcg gtattagctt aacaaacggc aaaatatgag gaactatttt tccgtttgtt
     1681 aagctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg gtattcaaaa
     1741 gcttgcatct gcagtaataa gaacagccaa gtaaaaacat aagaagctgt ttttacttgg
     1801 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttatgtac cttgtaggac
     1861 tcgaacctac gaccggacgg ttatgagccg tctgctctaa ccaactgagc taaaggtaca
     1921 tcataaataa aatttatttt tgttactttt caactatagc acgaatgaca aactctcaca
     1981 agtttcaaaa tataagtaaa ataatattgt tattcttatt tcttttactt gtgtcataac
     2041 tattttgtta ctttaatttt tatttgctta ttttagaaaa aatgttattc ttttatttat
     2101 tctcaatgtt aggagtgatt attttgccat ttattgcgat tttattcgat ttagttgcgg
     2161 cagcagctta ttttctccaa ttaaattatc aaacctcagg aattctgttt attggaatga
     2221 tttttcaagg tgtgattacc cttttgcttc ttgtcatgat gctcagctat aaaggaaaaa
     2281 gatatgcccg tattcaaaca aaagtatttg ttaaatatgt ttccattcgc tatgggatca
     2341 tcattctttc ctttttagtg aatgcgatcg tcctcttttt atatgtattg aattacttag
     2401 atatcaatcc attgattttt tctcgataga taagacacat tcagcggaac agttcctgct
     2461 tatcagcaga aaactgttct gctttttcat ttttcattga tctattcttc ttttattttc
     2521 ctagcatttc atttgtgatg tatgcaataa catgttatct tttattatat gaatcgttga
     2581 ggagtccgtt acatgattac gttggtgaga gttttgtttt gggtgcctgc ggtcgcactg
     2641 gtcgcaagta ttgtttatct tatgaattgg aacaaagaac ggttttattt agctattttg
     2701 actcttccgg ctatctactt tatgtggaaa gtttttaatt acaactactt tgaacccgat
     2761 agtgttttta tagaagaatt atcaggactg gttttgtctt tattgatcgt cattctttat
     2821 ttgatacgtt taaataaaaa acattgagta ctattgattg catgtgtagc tttctatagt
     2881 cattataact attctactat aatgaaaggt gattaaatga gtatcgaaga agctatttta
     2941 ctggatactt tacgttgtcg tctgaatttc ttaagaactg gccgatcttt atctcaagta
     3001 gctgtttcta gcgcgttaag tattccttta ttcaaatatc gtaagtacga agaaggagaa
     3061 gaaattccaa gcatcattga attaatctat atcgctgact tttacgatat ttccgttgat
     3121 tacctaattg gtcgctcaga aacagcaagc aaactgaagt gattttttcc agccttggtg
     3181 ctggtttttt catttaacgt ctaaggttaa caatctatat ataaaggaga catttctatg
     3241 cgattatggc atgaaaaatt gatttcaaag cttcctcgtc aacagctttt aggacaacat
     3301 cgggaatgct gtgcattaag aggaaatggc tggggaaaga aacatgctac tgtggattat
     3361 gtttttcact attctcctta tagattattc caatatcatc agcttgtcat ggatgaaatg
     3421 gaaaaacgtg gttacagccc agcaccagag tggaaaaatc ctatgtatcg tggcaaatca
     3481 tgcgaagcat tttttgattt acctactgta gagcgcaacg tgccgattta tccggaacac
     3541 gatgaagttt atctagcaga atgtttagca aatttggagc aaaaaggaat ttatcttacc
     3601 taatataact agaaaagcct tctatctcct cttacttcat taaaaagctt taaccatctt
     3661 agatcaagct aaaatgtcgt aagttttagg gttatcttca tcctcacgtg caaaactaaa
     3721 agcgtactct agttttggtt catcgccaac ttccctagac ataaatgaac atctcctgac
     3781 gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat gcacagcaca gattgcttct
     3841 tttgatcttt cttttgattt ttctgttgct tcttctacac cttcttcgat tactacatcg
     3901 atgcttacaa ttgcttctgc ctaatattcc catgcatcgt cgatttaagc ctctgtagtc
     3961 actgtgatcg tttccacatt ttaactcctc tgtcgtggct tcagttgatt ttgtgatcag
     4021 ttcagttgct aagccagagt cagaacaacc tgccattgct agcgttaata ttgatagtaa
     4081 aaatagtttt tttatcataa ggttcccccc ctgaaaatag actaaaataa taacgctttc
     4141 tttttttgtt ttcagacgaa aattaataat ttctaaatat tctttaaaaa agcttataac
     4201 tattatgcct tttttaatat ataacacagt tatttttaaa aataagaatg ctaaatcact
     4261 ctacaataac ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat
     4321 gaaagatcag gttttattag atctcattct ttcatcactt gaagggattt aatgaactac
     4381 tttttcctct ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt
     4441 caatgcttga tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta
     4501 gaacatattc ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt
     4561 tcgataggat aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa
     4621 gataggaatc tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca
     4681 cgctccacaa aattgtttta tacactacta ttcagcccat tcaacgacca atg
//
LOCUS       DARDHD010000173         4635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000173 DARDHD010000000
VERSION     DARDHD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.176"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..519
                     /locus_tag="SDA06_002390"
     CDS             175..519
                     /locus_tag="SDA06_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG4229642.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="SDA06_002391"
     CDS             520..939
                     /locus_tag="SDA06_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG4229643.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="SDA06_002392"
     CDS             complement(1031..1381)
                     /locus_tag="SDA06_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229644.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="SDA06_002393"
     CDS             complement(1347..2567)
                     /locus_tag="SDA06_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG4229645.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="SDA06_002394"
     CDS             2933..3499
                     /locus_tag="SDA06_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4229646.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="SDA06_002395"
     tRNA            3644..3731
                     /locus_tag="SDA06_002395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="SDA06_002396"
     CDS             4065..>4635
                     /locus_tag="SDA06_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG4229647.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DARDHD010000174         4590 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000174 DARDHD010000000
VERSION     DARDHD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.177"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..306
                     /locus_tag="SDA06_002397"
     CDS             <1..306
                     /locus_tag="SDA06_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229648.1"
                     /translation="YTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIELIKQNEMEVII
                     QELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFCYLLLLHEHN"
     gene            complement(387..722)
                     /locus_tag="SDA06_002398"
     CDS             complement(387..722)
                     /locus_tag="SDA06_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229649.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(789..2159)
                     /locus_tag="SDA06_002399"
     CDS             complement(789..2159)
                     /locus_tag="SDA06_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG4229650.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(2237..2773)
                     /locus_tag="SDA06_002400"
     CDS             complement(2237..2773)
                     /locus_tag="SDA06_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4229651.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            3082..4563
                     /locus_tag="SDA06_002401"
     CDS             3082..4563
                     /locus_tag="SDA06_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229652.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         1493 a          905 c          672 g         1520 t
ORIGIN      
        1 tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat
       61 actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg
      121 gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca
      181 ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt
      241 ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat
      301 aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat
      361 gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg
      421 tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt
      481 cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca
      541 tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact
      601 ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct
      661 gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc
      721 ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac
      781 taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact
      841 tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca
      901 tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag
      961 caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga
     1021 ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa
     1081 ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct
     1141 tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc
     1201 actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc
     1261 agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt
     1321 tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga
     1381 atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct
     1441 tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt
     1501 gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac
     1561 tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc
     1621 acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc
     1681 actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc
     1741 ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt
     1801 gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca
     1861 gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg
     1921 aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat
     1981 tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga
     2041 aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct
     2101 aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt
     2161 ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac
     2221 tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg
     2281 tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat
     2341 ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga
     2401 tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta
     2461 aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata
     2521 agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa
     2581 cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa
     2641 tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc
     2701 catattcttc cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta
     2761 ctctccccat cattcttacc tccgtctctt ttcatctacc cataaataaa taacataaat
     2821 aattctaaaa atattattga taattatcaa aacatattta gttatatcca ttaaaaatca
     2881 atttttccgc cttaaaaaca agaatatctt gacaaaatac cctaggaaat aaacaataga
     2941 ataaacaagg taacaaaaaa attaaataac gttatataaa aaagaaggcg acactctgtg
     3001 agaataacac agactgaaat gactttccac tcacagtttt tgtctaacta tatacaaaaa
     3061 cagaataaaa aggaggtatt tatgctaatt gaagcaagaa aaaaagagtt acttgttcta
     3121 caaatggtca ttcaaaaaaa taattgttct ttacaagaat tatccaatga cttacatatt
     3181 ccaaaacgga caatcaaaga actgatccga aaaataaatg tatcactcaa gcaattttta
     3241 ttgattgatg aatttattta ttctaatcat aaaggagaga tccaaaccaa agaaaccgat
     3301 caaactaaaa agttagctgt tttttctaag ttaaaattat gctacctaaa agaatccaat
     3361 cgttttaact atttgatgtt actaatcaat tatccacaaa ctgctgtacc aaaaaaatat
     3421 ttacttgaac aactttatat atcgccctcc tatctcgaaa aactgacaaa acagctcaat
     3481 cattccttaa aaaaattcca tttacggatc ctatctgtaa aaaattgctg tactcttgaa
     3541 ggaaatgagt tatctatccg tctctatctt tacttttttc tttcagatac atttggaggt
     3601 aacgattggc cattttcctc acttgaatta aatgcactaa aaatgaacag tacagatgag
     3661 caaatagaaa aaatttcaaa acaagaaaaa aatatctact tgctgttaac tttattcata
     3721 ttacgaaaca aacaacaggc atattttcca aaaacttctt ctgaaatcac tgaattgatg
     3781 gacttattgc agcatacaca tgattattca gaatacttta cttctttttc agtgagtcgc
     3841 ccttgtgaag agacagaaac agagagagcc tactttaatt attttttcta ttgttttttt
     3901 ccaaatcaaa tctcttttcg acaaaaacag gagattggtt ttttattttc cacaagcaaa
     3961 aaccttattt gtcgacaagt cggtcaattg atgacttact taactcaaca gtttcctctc
     4021 aaccatttag aaaataaaat accaatttat ttttattttc ttgtgttggc agttgttttt
     4081 ttcgaactgt tgggtgaatc cagcagtagt ttttctacac tttgctttcc agaagccacg
     4141 tccaatcatt ctagttcttt tatacaggaa aagaaattaa gagaaaaaat cgatacaatt
     4201 ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc ttagtcaatt gatccaacac
     4261 ttactacaaa cagaaacgaa agagcagtta ctcatccatt tgcagataac caaacagttt
     4321 acaggaaatt atcatcttca aagccatttg cagcagttat ttcgcaaaga tgctgttaaa
     4381 attaccgcat attcagaaca tgctgatttg atcattacag atcagcatga gaaaatttca
     4441 gatgaaacgg agactttcta tctggataca acgcacaata cagtgttatt tggcaagtta
     4501 ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg agaacaaaac aaagaggatg
     4561 tgacaaaatt cttgtcacat cctcttattc
//
LOCUS       DARDHD010000175         4490 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000175 DARDHD010000000
VERSION     DARDHD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.178"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..219)
                     /locus_tag="SDA06_002402"
     CDS             complement(<1..219)
                     /locus_tag="SDA06_002402"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4229653.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="SDA06_002403"
     CDS             complement(330..584)
                     /locus_tag="SDA06_002403"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4229654.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="SDA06_002404"
     CDS             complement(777..1052)
                     /locus_tag="SDA06_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG4229655.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="SDA06_002405"
     CDS             complement(1024..1977)
                     /locus_tag="SDA06_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4229656.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="SDA06_002406"
     CDS             2589..4082
                     /locus_tag="SDA06_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG4229657.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..>4490
                     /locus_tag="SDA06_002407"
     CDS             4217..>4490
                     /locus_tag="SDA06_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG4229658.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1464 a          750 c          786 g         1490 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DARDHD010000176         4470 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000176 DARDHD010000000
VERSION     DARDHD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.179"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            318..1049
                     /locus_tag="SDA06_002408"
     CDS             318..1049
                     /locus_tag="SDA06_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4229659.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="SDA06_002409"
     CDS             1194..3221
                     /locus_tag="SDA06_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4229660.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="SDA06_002410"
     CDS             3329..4426
                     /locus_tag="SDA06_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4229661.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DARDHD010000177         4469 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000177 DARDHD010000000
VERSION     DARDHD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.180"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..743
                     /locus_tag="SDA06_002411"
     CDS             54..743
                     /locus_tag="SDA06_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229662.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            749..1705
                     /locus_tag="SDA06_002412"
     CDS             749..1705
                     /locus_tag="SDA06_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG4229663.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            1749..2261
                     /locus_tag="SDA06_002413"
     CDS             1749..2261
                     /locus_tag="SDA06_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229664.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(2517..3065)
                     /locus_tag="SDA06_002414"
     CDS             complement(2517..3065)
                     /locus_tag="SDA06_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229665.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(3154..4335)
                     /locus_tag="SDA06_002415"
     CDS             complement(3154..4335)
                     /locus_tag="SDA06_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG4229666.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         1531 a          843 c          831 g         1264 t
ORIGIN      
        1 taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga
       61 ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct
      121 tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct
      181 tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag
      241 attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa
      301 gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc
      361 gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag
      421 cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt
      481 tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaaaag
      541 atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac
      601 gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag
      661 aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt
      721 tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt
      781 tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc
      841 agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa
      901 aaagcaccat ccaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga
      961 tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt
     1021 aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg
     1081 acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt
     1141 tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg
     1201 tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt
     1261 tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat
     1321 cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac
     1381 gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc
     1441 tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat
     1501 gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca
     1561 gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga
     1621 acttattagt cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca
     1681 ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag
     1741 gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca
     1801 ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga
     1861 aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag
     1921 tggaagatca aattgctgga acagctgcat tagtctcagg cgtggatccc gtttacacaa
     1981 aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt
     2041 tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga
     2101 ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga
     2161 tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa tttccttttg
     2221 aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca
     2281 aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac
     2341 agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt
     2401 ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg
     2461 tgacatagct tttgtcacga tctctcggct tatcctacga gtattattcg atacatttat
     2521 atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg
     2581 actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc
     2641 gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat
     2701 gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa
     2761 tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta
     2821 atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca
     2881 tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct
     2941 ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa
     3001 atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa
     3061 ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca
     3121 aagcttttaa aagtaacttt gcttttttgt attttatacc agcaagccag tcacaatagc
     3181 acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag
     3241 atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa
     3301 gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa
     3361 attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat
     3421 gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc
     3481 aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt
     3541 gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg
     3601 gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc
     3661 aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat
     3721 ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc
     3781 catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa
     3841 caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg
     3901 tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt
     3961 acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat
     4021 accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact
     4081 tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag
     4141 atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa
     4201 tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc
     4261 ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc
     4321 taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc
     4381 aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat
     4441 tttccattag aaaataaagc gaacaaaat
//
LOCUS       DARDHD010000178         4436 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000178 DARDHD010000000
VERSION     DARDHD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.181"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(72..389)
                     /locus_tag="SDA06_002416"
     CDS             complement(72..389)
                     /locus_tag="SDA06_002416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229667.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(416..622)
                     /locus_tag="SDA06_002417"
     CDS             complement(416..622)
                     /locus_tag="SDA06_002417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229668.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(603..785)
                     /locus_tag="SDA06_002418"
     CDS             complement(603..785)
                     /locus_tag="SDA06_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229669.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(799..1149)
                     /locus_tag="SDA06_002419"
     CDS             complement(799..1149)
                     /locus_tag="SDA06_002419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229670.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1175..1477)
                     /locus_tag="SDA06_002420"
     CDS             complement(1175..1477)
                     /locus_tag="SDA06_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229671.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(1480..1713)
                     /locus_tag="SDA06_002421"
     CDS             complement(1480..1713)
                     /locus_tag="SDA06_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229672.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(2699..3021)
                     /locus_tag="SDA06_002422"
                     /pseudo
     CDS             complement(2699..3021)
                     /locus_tag="SDA06_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3144..4259)
                     /locus_tag="SDA06_002423"
     CDS             complement(3144..4259)
                     /locus_tag="SDA06_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4229673.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(4265..>4436)
                     /locus_tag="SDA06_002424"
     CDS             complement(4265..>4436)
                     /locus_tag="SDA06_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229674.1"
                     /translation="NVSEEEIEEIKNELEDYDLLTIEFGRIYLKELIATMEDVRVYQH
                     QNSFLIYGQDEV"
BASE COUNT         1217 a          950 c          577 g         1692 t
ORIGIN      
        1 accctcttgt tatttttatc acataatcat tttataaaaa cgcttcgaaa atatcaacat
       61 ttttgaagaa actatttttc taatttctca atttcttcca agggattttc gctaatgacc
      121 catacaggca cagattgctc tcgaggctcc ccttcttcta accaccaacc tgcataatga
      181 acttcttcct cctcagcatt accatgatac caaccaacaa aggattcatc tacaacgaat
      241 gaaatttctt cttctggatc aaataattca tttttccatg cttcaattgc ttgttctttg
      301 gtgtactttt gtttgttaaa tacaccacaa atttcgtcat ctacataaaa aatttcaaaa
      361 tcaaaattac tcgttccttt tactggcatc ttaacttttc ctcctttgac tcacttcaat
      421 agatatacca ctcatgttca ttccattgtt tactagttaa ttggagtttt atcttagggc
      481 tttgtgcact ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat
      541 ttcttacatt cggtactaaa tgtttttcct gcatttcttc atgaaaaaca tataattctg
      601 cttcattttt caaccaattc attattcatt ccctcattca cttcttcata ccgccattca
      661 gatagtgaga tttctttttg tttctccact tgtcctataa aaatcgctgt attcaacaag
      721 ctgatcacac ttaataataa agcaatgaaa ctaatggtta cacttactct ttcacatttt
      781 gtcattttgt ttccttcttt actcgtttgt tatttcttgt aataggtcta ataactcctt
      841 caccgaaatt tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac acattaccag
      901 accttcaact gatatatcac tgcgacaata aaattctgga gaaacactga tttctgattc
      961 tttcttacaa ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt cttccacaac
     1021 tatgttttgg gattctacca agaaagggag tgcctgttct aatgcttctt ctggtgtttg
     1081 agcagataga ataattgctt cttgttcttt tatttcattg ttctcaaata ctttataact
     1141 ttttttcatt gttagttcct ctttcattcc tctctcaatt agctaagccg ttactggtac
     1201 gccattctct aaatttagta tcgctaatcc ttaatgctcg tcgaatatca atcattcgat
     1261 aacctattgc acgaagttct ttatatcttt tagcagttag cgtctctaac gtcatttcag
     1321 gcaatgggtt cgaatcaaaa atagaattcg aaccgattat ttgggccaat tgttgtactt
     1381 gtaacagctc ttctgaactt aattcagtga ttgctcgatt gcctatcatt gcatacattt
     1441 tctgtcgaat atacttcttt tcttgtttag acaacatttc tatccactcc tattttcatt
     1501 atcatttgtt acctccactt cagtaaatcc acaaataaat gaattcatat tccaagtacc
     1561 attcccttca actacaactt ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt
     1621 taccaatatg tttttcacta ctactaattt gtttgtatac cgtggaattg ctgttccgat
     1681 atacttcact ttatttccta cgctaattgt catttcctca ctcctattct tttttatcca
     1741 aaactttttc ctgtcctatt cctgccaacg aatatacttc actgcatgct taaacaaaac
     1801 aaaatgttgc cctgttttat ttccgtcttt tacttgttcc acatacagtt catatttggt
     1861 cacacctttt aaaacgccag atactttttt tcatccattg tcacaattag ttttttcatc
     1921 aatcgattga tactgcactg ctcatttttc ttgttcatat agtattcctt tctttttcaa
     1981 aactagcctc ttttgtttac tagttttcat caatcatcaa cgatttaaca gattttttct
     2041 ttcgcagggt ttctagcgtc agcaacccca aactctaaaa aagtgccttt gctcccttga
     2101 aaaacgagcg gacgattgta ctcgtcatca tatgtttttc atactacctg atccacgcaa
     2161 ataacctttt tttgttctgt gccacaccca tccatacgta tcgcttgaga ttcaccatac
     2221 ttttttaggt ggtttatcct tcctaagtcc ctgttagaca cgctgattaa cttagaaagt
     2281 cctgtttgta tccaattttc aaagatctcc caaaagaaat aagtaggttt acgtgtatcg
     2341 cttctgttca aagtacctgt ttgtacttga gtatttctac tcccctcgtt caagggattc
     2401 cttccaccag ctagcttttc ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg
     2461 tgtccgtgtg cttacttatt cttttttcga tagcgattat ccgctcgcta ctcgctattt
     2521 tatactttgt ttgttagctc aaataaaaac taacaaacaa aaaaggctag tttctttccg
     2581 ttttattttt ttaactgtcg ataaatagtt gttagtttaa ttccaagttc taaaaccaat
     2641 acgcccaaca aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc
     2701 atgtcttccc tctttctttt tggtgtcgct gtggtcgttc ttgccgttca atacttcgat
     2761 acatttttta ttcattattt gctcgttttg ttttcctctg gactgctttt cttgcttttt
     2821 gaatacttta tacgcttttc gtgcctgaat taataggtct tgtcggcgtt tcattcgtga
     2881 gaaaacaata tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg caataatttt
     2941 cccatcaaaa aaaatagttt tcacttgaga aaaaaacagt tggaaatctt gttttgttgc
     3001 taattttttt gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac
     3061 aatccgattc gataacttcc aacgttgccg attaaacaat gttacttcac aacaaatatg
     3121 acgtaagtga ttaggggtca ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa
     3181 attttgggca attgtgacag cccataaatc tgcgtttcca ggtcttcata actacaagaa
     3241 cgcttggctt gaagtaatga atattctgct tgatctgata gttttgttgc cattggatct
     3301 agattcaatt ctcttacaca acattcccaa aaattcaata gcgctctaaa ataaaatcct
     3361 tttcgattct gaattgcttt tccttcatga cgagcaattt tttcattttg aatacaccgt
     3421 tgatacgttt tgcttaaatc ttctgcaaat gtttccccta aaattcgata ctcgcttcca
     3481 tcagataaac catgtgtttg attatacttt cgttgccgct tttctataat tcgtttactg
     3541 tcgtatatca tgtttagctg ctctttcgct tcacgccatt cagctgaact gcgaacaatc
     3601 catgtcaaaa catctttcgg aacaaacgta ctttcgctgt aatgagcaat aaactctttt
     3661 tgccaatcat cagccatttc aacaggacta gtcgtatcga tttttgtatc tagattttct
     3721 gtatctagat tgttagaaga attattaaat aattgtgcag aattttcaca tggatttttt
     3781 tcgtcaaaat catcgaaaat cgctaaatta tcaacagatt cctcctcttc ttttccttgt
     3841 gcagaatcta cactggttga gtcaacagga cctaatgaat acacatcatt ttctgtcgta
     3901 tctaattctc ctagatacaa ccgagctttg tttctttttc ctgtttgttg atcaaatccc
     3961 atatcctcta tttctagtag ctcatattct ttcaattctt ttttgattgt attaacagaa
     4021 cgtctgcaac aattcaatgc attggctaat tcttgttccg taaattcaaa ataaacaaaa
     4081 ccttgactat ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata
     4141 tacgcgattt ttgctttatc agacaatgag cgatattttt cacttgtaat aaataccttt
     4201 ggaaattgat agtataattc attgtattta cgagttgcgg taaatctttt tgttgccatc
     4261 gttattacac ctcatcttgt ccatatatta agaaagaatt ttggtgttgg tagacacgaa
     4321 catcttccat tgtagcaatc aattctttta gataaattcg accgaattca attgttagta
     4381 agtcgtaatc ttctaattca tttttgattt cttcaatttc ttcttcagac acattg
//
LOCUS       DARDHD010000179         4334 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000179 DARDHD010000000
VERSION     DARDHD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.182"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..338)
                     /locus_tag="SDA06_002425"
     CDS             complement(<1..338)
                     /locus_tag="SDA06_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229675.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="SDA06_002426"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="SDA06_002426"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG4229676.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="SDA06_002427"
     CDS             complement(1495..2922)
                     /locus_tag="SDA06_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4229677.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="SDA06_002428"
     CDS             complement(2953..4167)
                     /locus_tag="SDA06_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG4229678.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1367 a          922 c          770 g         1275 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaac
//
LOCUS       DARDHD010000180         4324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000180 DARDHD010000000
VERSION     DARDHD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.183"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SDA06_002429"
     CDS             complement(<1..281)
                     /locus_tag="SDA06_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4229679.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="SDA06_002430"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="SDA06_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="SDA06_002431"
     CDS             complement(2359..2721)
                     /locus_tag="SDA06_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229680.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="SDA06_002432"
     CDS             complement(2725..3063)
                     /locus_tag="SDA06_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229681.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="SDA06_002433"
     CDS             complement(3042..3461)
                     /locus_tag="SDA06_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229682.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="SDA06_002434"
     CDS             complement(3454..3687)
                     /locus_tag="SDA06_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229683.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="SDA06_002435"
     CDS             complement(3684..>4324)
                     /locus_tag="SDA06_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4229684.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DARDHD010000181         4323 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000181 DARDHD010000000
VERSION     DARDHD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.184"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            97..567
                     /locus_tag="SDA06_002436"
     CDS             97..567
                     /locus_tag="SDA06_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG4229685.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            652..1260
                     /locus_tag="SDA06_002437"
     CDS             652..1260
                     /locus_tag="SDA06_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG4229686.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1394..1594
                     /locus_tag="SDA06_002438"
     CDS             1394..1594
                     /locus_tag="SDA06_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4229687.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2348..3574
                     /locus_tag="SDA06_002439"
     CDS             2348..3574
                     /locus_tag="SDA06_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4229688.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3682..4152)
                     /locus_tag="SDA06_002440"
     CDS             complement(3682..4152)
                     /locus_tag="SDA06_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG4229689.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1529 a          628 c          853 g         1313 t
ORIGIN      
        1 aaaggatcaa aaaaaattaa tttttttaaa taaagatgaa agttattgaa atatagagta
       61 tgataaataa gaatttttaa taaggtaggt tggctcatga agcagtatcg acagttaaaa
      121 ggatatttag aagcaaaagg atattgggag atactagtct atctcttttt tggtggtttg
      181 gctacgattg taaattttgt tagttttgct ctcgcacgcc aatattttga tttaagtatg
      241 gccttatcca attctatttc gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa
      301 ctttgggtat tccattcaaa atcaccgaat tttacacatg cacttattga atgcggtaag
      361 ttcttcttct accgtatcct ttcttacgga ttggacatgg gggcaatggt attgttgatc
      421 aatgttatga acagcaatga gtatgtggcg aaaatcatca ctcaaattat tgttatcctt
      481 gcgaactata ttttcagcaa gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa
      541 gtaggtcaaa gtgaaaaaga cgactaggca taacaattga agaatttcct tctctttgtt
      601 actctttgta atgtagacat tattaagaaa acattgagga ggaatttttt tatgacttac
      661 acattaccag atctacctta tgcatatgac gcattggaac cttatattga cgaagaaacg
      721 atgcatctgc atcatgataa gcatcacaat acttatgtga cgaatttaaa ttcagcaatt
      781 gagaaatacc cagaattagg cgaaaaaaca atagaagaat tattatctga tatggacgct
      841 attccaacag atatcaagac agctgtacgt aacaatggtg gcggacatgc taaccattca
      901 tttttctggg aaatcatggc accaaatgct ggtggcgaac ctacaggaga aataaaagaa
      961 gcgattaatg aagcttttgg tgatttttct tcttttaaag aagaattcaa aaaagcagcc
     1021 gctggacgat ttggttctgg atgggcttgg cttgtaatgg aaaatggaaa attagctatt
     1081 acctctactg caaatcaaga ttctccattg atggaaggaa agacaccaat tctaggtttg
     1141 gacgtttggg aacatgctta ttatttgaaa tataaaaatg tccgaccaga ttatattgca
     1201 gcattctgga atgttattaa ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa
     1261 ccatttcctc cgtcatatca gtcttaaaaa taatttggaa aataaaagag cgaaagcttg
     1321 ctagcgtttt ctaaattggg tataatgttc ttgcaaggtt acttgcaaga acttttttgg
     1381 aggatataca tttatggaac acggaacggt taaatggttc aacaatgaaa aaggatttgg
     1441 gtttattaca gtagacggcg gagatgacgt atttgttcat ttctcagcaa ttcaaggaga
     1501 cggcttcaag agcctagaag aaggtcaaga agtagaattc acgattgttg aaggtgcacg
     1561 cggacctcaa gctgctgaag tgactaaatt ataataattt gttgaataaa gacacgactt
     1621 cttagagagt cgtgtctttt tgtctattta tgaagttttt agattttttg gctaagtaaa
     1681 tgcatttgtt cacttgacat gtcctaacta attatcaagc aaggaacatt ttcttcgtta
     1741 gtaatggatt ttaaagaaat ttcagttttt ggatgttgaa taaaagacca tttgtgaaac
     1801 cactagtata catactcatt accatgtcaa ttctattact ttaaaagaaa atcgttttac
     1861 tttgaagagg agcatatcta tccattaaaa gatgatggta atgcaatcac tagttcgagt
     1921 cattttaaga gccatgcagt gagctggaaa taaaacgaat agaagatggg tttaacaaaa
     1981 ggaccagtca taggaagaaa ttgtcgtgaa atcgaatgat aatctgaacg gtaggcaaag
     2041 actgttaaaa agatgggaaa ggtatgtgcg atcattttac tgcacgatta tttggaaaat
     2101 accttgttgt tcgctttttc tggaaaaaat acataaaaaa agataaaacg attgcgaact
     2161 gcgtgtattc ggtctttcga tctttttcac ggtatggtat gatagttaag aattttgaga
     2221 aattttaaaa aataataatc gttacacaaa aatgtttttt aaagttttct tatgattaaa
     2281 aagtgaattt gctgattgaa cagttatatg agacaatatt ttagaaaatg tgaaacagag
     2341 gtgtattatg aagcgatggc aaaaagttct attaaccttg ttaggggtat tcgttttgct
     2401 gattgccgga ttatcagcat acggaatcag atttatggga gaagcgagcc aaacagtaaa
     2461 caaaatttct aaaaactcta atcgaatatc ctccaaacgt tctgagagag taagcattga
     2521 tgacaaagaa cctttttcgg ttttgctatt aggtttagat accggtggat taggaagaac
     2581 ggaacaagga cgttctgata cgatgatggt agtaacagtt aatccgaaac aaaaaaaatc
     2641 aacgatcatc agtttggacc gagatattta tacgaatatc gtaggttatg gaacggtaga
     2701 taaattaaat catgcctacg catttggtgg tgtcgaaatg gccatggatt caattgagca
     2761 actgctcgat attcctattg atcattatgt aacgatcaat ttggatggca tggaagattt
     2821 gatcaatgcg gtcggtggca tcaaagtgaa taataaaatt gactttactt tggatggtgt
     2881 ccatgtacca gtaggtgaac aaacattgaa tggagaaaaa ggtttagcct attctcgaat
     2941 gcgacacgaa gatccagaag gcgatatcgg tcgacaacgt cgtcagcggg aagtagtgac
     3001 gaaaatcgtc aataaagttt taagtttaga tggcgtaagc aattatagaa aaattttaaa
     3061 agcagttgag aacaatgtga cgacggactt agattgggat gacatgcttg atgtagcgac
     3121 gaactacacc ccggcgtttg aaacaatcaa acaagaccag ttgcaaggcg aaggacaaat
     3181 gatcaatgat atctactatc aaattttagg aaaaaatgat cttctttcga tccaaaatga
     3241 actgaagaaa caattaaata taaaaacaag cgatacatta cctaatttga aaaatgagaa
     3301 tgcttcaatg atgttctttg atgattcgga agatggcgat tcgacaaacg gtgatggatc
     3361 gaatagtgaa gggtacaccg attattcttc ctattcagaa aacacatatg atcagtacca
     3421 agatccgaat acctattatc agcaaacaga agatacaagt cagtactata caccaagtca
     3481 agatactcaa agttatgatc cttacgctgg acaggaacaa aatggttatg gttatgacca
     3541 gaataatgag tatggaacaa caagtgggta ctagaaaaaa atagtttggt taataaagag
     3601 tgccacctaa attagacaaa aaatctaact taggtggcac tttttctatg tgttaaatta
     3661 caggacgtct ctttttagcg cttagtttaa aaaataaatt gaataattca atagagttgc
     3721 gaatagtacc cataaataat aaggaatcat gagccacatt gtcaggcgat gatgcagcca
     3781 caactgatac ataagcactg acaagaaaat aactaaaagt gtaatatcga ttgctgcaat
     3841 gaaaatattt cttaaattaa aaaatagagc agaccatata aaattaaaaa tgaactgaat
     3901 cacaaaaaga ccgacaagat tgtttcgctg ttttttgttg ttgtgattaa gtaacaaata
     3961 aagagaaata cccatcaata tatacagtaa ggtccacatc ggcccaaata tccagctagg
     4021 tggtgcaaat ggtggaagtt gcaaagaata gtaatatttt ccgggatttc cgctaagcaa
     4081 ccctgataaa aagcctaata tgacaattcc tataacacaa acccaaaaac gaatatcttt
     4141 tatcatcttc attttactaa ctcctttcta taatattagt ttaccatgaa ggctcacagc
     4201 aagacctgta tttagtttgt gaagtaagaa aaaaattgat aattgttgtt attaaaaagt
     4261 tgaacatttg tttatatgaa tgatgttttt gaaaaagatc atatgatttg attctcatgt
     4321 ttt
//
LOCUS       DARDHD010000182         4310 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000182 DARDHD010000000
VERSION     DARDHD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.185"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..1285)
                     /locus_tag="SDA06_002441"
     CDS             complement(65..1285)
                     /locus_tag="SDA06_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG4229690.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="SDA06_002442"
     CDS             complement(1305..1916)
                     /locus_tag="SDA06_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4229691.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="SDA06_002443"
     CDS             complement(1959..2891)
                     /locus_tag="SDA06_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG4229692.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="SDA06_002444"
     CDS             complement(3312..4172)
                     /locus_tag="SDA06_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229693.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DARDHD010000183         4298 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000183 DARDHD010000000
VERSION     DARDHD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.186"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SDA06_002445"
     CDS             complement(<1..173)
                     /locus_tag="SDA06_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229694.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SDA06_002446"
     CDS             complement(593..1714)
                     /locus_tag="SDA06_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG4229695.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SDA06_002447"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SDA06_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG4229696.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SDA06_002448"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SDA06_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG4229697.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1418 a          776 c          812 g         1292 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatct
//
LOCUS       DARDHD010000184         4239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000184 DARDHD010000000
VERSION     DARDHD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.187"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SDA06_002449"
     CDS             complement(221..1423)
                     /locus_tag="SDA06_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4229698.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SDA06_002450"
     CDS             complement(1426..1620)
                     /locus_tag="SDA06_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4229699.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SDA06_002451"
     CDS             complement(1667..2515)
                     /locus_tag="SDA06_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG4229700.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SDA06_002452"
     CDS             complement(2720..3070)
                     /locus_tag="SDA06_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229701.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SDA06_002453"
     CDS             3665..4111
                     /locus_tag="SDA06_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4229702.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT         1233 a          733 c          794 g         1479 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaac
//
LOCUS       DARDHD010000185         4181 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000185 DARDHD010000000
VERSION     DARDHD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.188"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..528
                     /locus_tag="SDA06_002454"
     CDS             <1..528
                     /locus_tag="SDA06_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEG4229703.1"
                     /translation="NSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIE
                     DLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGI
                     KVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRY
                     QRGIKNLVKSYLKMN"
     gene            531..1427
                     /locus_tag="SDA06_002455"
     CDS             531..1427
                     /locus_tag="SDA06_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229704.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1477..1866
                     /locus_tag="SDA06_002456"
     CDS             1477..1866
                     /locus_tag="SDA06_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229705.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1853..>4181
                     /locus_tag="SDA06_002457"
     CDS             1853..>4181
                     /locus_tag="SDA06_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4229706.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1431 a          648 c          879 g         1223 t
ORIGIN      
        1 aactctgatt tgatttcaga gtataaaaat gagattgaaa catgtgtaaa cgaaataaga
       61 aaattaaatt gggatattgt ttctgtgaaa aaagaaatac aagataaggc gaaggtagag
      121 cttgaaattg aagatttaaa aaaacaagta gaaaagctat ctcataagag ttataaagat
      181 gtttatgatg agtattcaaa agagacagat atttataggg aactgaaacg tgatgtggag
      241 gcacttaagg aaattcccaa aaatctaact gatagtgttg acaaaattga ctttggcaat
      301 ggtataaaag ttacagacga aattgatgaa caccgcggag aattgattaa gaatttagtg
      361 accaatcggg ctaagattca aacaataatt aatgaaatgt cagatgaaat tgatagttat
      421 agagcagagc tgaataaatc agattggaaa gttaactata atgaggtttc aaagaggtat
      481 caaagaggta tcaaaaactt agtgaagagt tatctcaaga tgaattaaaa atgttacaaa
      541 accttgattc tttatatcaa tctttatcaa aaactgaaga gcgattggaa gaaattattc
      601 aacaagaaat aaagcttaaa gattaccaag ttgaggttga ctcagcaatg tcaagaatga
      661 ataaatcata tcaaaatctc tattctgaga gaagtagttc ctcgcgcgaa attttttctg
      721 atattacagg aatagaggct actattcaac caaataggga tttcaataat tatgttaaag
      781 agctcagagc ttgtattaat aaggaggaca cttttgacag tgagtttgga tctcttattg
      841 ataagctgac aaaaaattcc attactaatc aagatatata taatgacata cttgatataa
      901 gagttagtgg ggaaagtagt attttttcag ataaaaaatt aatcaattct ataagaaaat
      961 tgaatgatgc acaagttctt gaattacaaa cccttaagcc aaatgattta gtagttatcc
     1021 gacttgaact aaatggaaaa aaagttagtt tatcgaatgc tagtgctggt caaaagacat
     1081 ctgcaattct ttctatgatt ttggcgtatg gaaattcacc actcatattg gatcagcctg
     1141 aagatgattt ggatagtcaa ttaattaata acttaatcgt aagaagtatc attacaaaaa
     1201 aggaaaacag acaaatttta attgtaactc ataatgcaaa tatcccagtt aatggtgatt
     1261 cagaatggtt gattagcatg ggagattcaa aagaaatatc ggctgatcag tgcgggtcgg
     1321 tagatgaacc caatatcaag ccaagaatat gtactgtgat ggaaggtggc gaggatgctt
     1381 ttacaaatag agcgaaacgc tatggtttta aacaaattgt agaataacac tagagggtga
     1441 gacatattat tatgtccgcc ctttttagga ggaaaaatga aaaaaataaa aagctatacc
     1501 agtatctggg cagttgaaaa agtcatttac gcaatcaatg attttcagtt gccttttcct
     1561 gtgactttta accaaatggc ttggttcgtt ttatctttac tgtttgtcat cgtatttgcc
     1621 catgtgcctc cactatctat gatagaggag gtctttttaa aatacttagg cataccggtg
     1681 gctgtcactt ggtttatgtc gcaaaagacg tttgatggca agaaaccact gggattttta
     1741 cgatccttta tcagctatca cttgcgattt aaggtgactt ttgcaggaaa gaaagtcaaa
     1801 gaacagaaga aacgatggga tgaacccagt actcttgtaa ggagtgtgaa ctatgtatcc
     1861 gattaagtat attgaaaata atctcgtctt taaccaagaa ggcgagtgtt ttgcctacta
     1921 tgaattagtg ccttacaatt actccttttt gtcacccgaa cagaaatttc aagtgcatga
     1981 taactttcgc caactaattg cgcaaaatcg tgaaggcaag atccatgcgt tacaaattgc
     2041 cacagaaagt agtattcgcg ccacacaaga acgatcaaaa aaagaaatta ccggtagact
     2101 caaagaagta gcgaaacaac gaattgatat acagacagat gcattagtat ccatgattgg
     2161 cgatagtcag attgattatc gcttttttat tggctttaaa ctaattgcga cagatgaaga
     2221 agtcaatcta aagagtctca agaaatcttt cttttctggg cttcaagaat ttgtctatgg
     2281 agtgaatcat catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgctgcta
     2341 ttctaagctt gaaaaattaa tggaaagcaa attggctcga agatttaaag tcaggcgagt
     2401 cacaccgagt gacctgacgt atttgattga acatatttat ggcgaaaaag ggataccgtt
     2461 tgaagaatac gaatttcagc taccaaagaa aaagctaaaa tcagaaacac tggtgaaacg
     2521 ttatgactta ttacgtccaa atcgttgttt aattgaagaa aaaccaaggt gcttacggat
     2581 ggaacatgag aatcatgagt cgtacgtagc gtatttaaca attaatacaa ttgtggggga
     2641 gatggaattt ccttcctcag aacttttcta ttaccagcaa caacaattta cattcccaat
     2701 cgacacaagt atgaatgtag agattgtgac gaataaaaag gctttagcta ccgttcgtaa
     2761 taagaaaaaa gaactgaaag atttagacaa tcatgcttac caatctgaca atgaaacgaa
     2821 ttccaatgtc ttagatgctt tggattccgt ggatgaattg gaaaccacct tggatcagtc
     2881 aaaagaatct atgtacaaac tgagttatgt ggttcgagtg agtgccgaat cagtggatga
     2941 actaaagcga cgctgtgatg aagtgctcga tttttatgat gataccaatg taaaactagt
     3001 ccggcccttt ggcgatatga tggggctaca tgaagaattc ttaccctcaa gtaaacgata
     3061 tatgaacgac tatattcagt atgttacgtc tgattttttg gcggggcttg gttttggggc
     3121 tactcagatg ctaggagaat tagaagggat ttactttggc tacaatgtgg atacaggtcg
     3181 taatgtttac ctgaaaccgg cattggcttc acaaggagtc aagggttcag taaccaatgc
     3241 gctggccgcc gctttcctgg gttctcttgg tggaggtaaa tcctttagta acaatctctt
     3301 agtctactat gctgttttgt tcggtggtca agctgtgatt gtcgatccaa aaggggagcg
     3361 aggagggtgg aaagaaacct tgccagaaat tgcggatgaa atcagtatcc tcaacttgac
     3421 gagtaagtca gaaaatcaag gtctgcttga tccttatgtg attatgaaga aaaagaaaga
     3481 ctcggaaagt ttagcaattg atattctgac ttttttaaca ggaatttcaa gtcgagacgg
     3541 ggagaagttt cctgtgttac gtaaagccat tcgaacggtg actcaaagtg atcaacgggg
     3601 gttattacaa gttattaacg aattgcgaaa agacgggtca ccaatcgcgg aaaatatcgc
     3661 cgatcatatc gagtccttta ccgattatga ttttgctcat ttgcttttct cagacggctc
     3721 gattacccaa tcgattagtt tagataaaca gctaaatatc attcaagtgg cggatttggt
     3781 tttaccggat gcagaaacga cttttgaaga atataccacc atggaattgc ttagtgtttc
     3841 gatgctaatc gtcatttcca cttttgcctt ggactttatc catagtaacc gagaaatctt
     3901 caaaattgtc gatttggatg aggcttggag ttttcttcaa gtggctcaag gaaaaacgtt
     3961 atcgaataag cttgttcgtg ctggtcgggc catgaatgct ggggtctatt tcgtcactca
     4021 aaatagcgac gatttattag atgaacgttt aaaaaataat atcgggttga agtttgcttt
     4081 tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggtt tggatcagga
     4141 ggatcaaagc aatcaaaaac gcctgcgaga tttggaaaac g
//
LOCUS       DARDHD010000186         4178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000186 DARDHD010000000
VERSION     DARDHD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.189"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            443..1411
                     /locus_tag="SDA06_002458"
     CDS             443..1411
                     /locus_tag="SDA06_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4229707.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1422..2237
                     /locus_tag="SDA06_002459"
     CDS             1422..2237
                     /locus_tag="SDA06_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG4229708.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2305..3015
                     /locus_tag="SDA06_002460"
     CDS             2305..3015
                     /locus_tag="SDA06_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4229709.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3051..>4178
                     /locus_tag="SDA06_002461"
     CDS             3051..>4178
                     /locus_tag="SDA06_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4229710.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1396 a          628 c          890 g         1264 t
ORIGIN      
        1 tcagttttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
       61 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
      121 ccagtagttt cctaattcaa ctgtgtgcaa aaacgatttc ttctaaacaa atcaagacaa
      181 acttataaac cttcatccca gcaccataat tatttaaatc ataggcattt ttcgaaatta
      241 gtatatgtac tcaagtttca ctgcagatta tttatatttt ttagagaaat aaatttttcc
      301 tattgacaaa cacaaagaaa gcggatacaa tgcaattatg tcacatgacg ttatatgacg
      361 ttatgtgata tctttacatt taatcagtta cttttatttt ggataaaatt aacattttta
      421 aaattttaag gaggaaatgt tgatggatgt agcaaaaacg gttaacgcaa ttgtagaaaa
      481 aaatgatgga gagatttctg gaatttattt ggtggcttgt ggcggttcgc tggtagatat
      541 gtatgtctca gattattttc ttaaagccga agcatcgaag tgtattacag gcttatatac
      601 agcgaacgaa tttaaccatg taaccccaaa gcaattagat gataaatctg ttgtcattct
      661 atgttctcat ggaggaaata cagcagaaac ggtaaaagca ggagaagtcg ctcaagctag
      721 aggagctgtg actgttggtc tgactcataa taaaactgcg gatttattaa aggtttctga
      781 tcatagtttc ctttacgaat ggggagatga tgcaaaaata gtcaataatc ctatggccat
      841 cattcttgat ttaactgtat ctttggtcaa tgttaccgaa acatttgaac attacggtaa
      901 attcagagaa ggcatgaagg ttatcgatac cgtaattgaa aatggcatta agcaggtgca
      961 ggatcgtatt aaagtgtttg ctgataagta caaagatgaa acaatgtttt atatcttagg
     1021 tagtggacca tcatatggac atgcttatgg tttttcaatt tgctctttga tggaaatgca
     1081 atggcttgat tcaacttctg ttcattctgg agaattcttc catggacctt ttgaagtaac
     1141 agacaaagat acgaatttta ttctcttgat gagcattgga cgcactcgtg ctttagatga
     1201 acgggcaaaa cgattccttg ataaatatgc agaaagagta gaaatcgttg atgcaaagga
     1261 actaggattg gatatgatat cagatgaagt ttctgaattc ttcaatccca ttttattcta
     1321 cagtattctt tcagaatatc gttcagcttt agcagatgta cgcaatcatg atttagatgt
     1381 tcgtcgctat atggggaaag tggagtacta ggaggattaa aatgaaggta ttaggcttag
     1441 gtgataacgt cgtcgataaa tatgaaaatc tgggaattat gtatcctgga ggaaattcat
     1501 tgaattttgc agtttttgca aaaaaactgg gacatgaaag tagctttatg ggagtttttg
     1561 gctcagatga ggaggcagct catgttttat cagcaataaa agaaatagga ctggacaaca
     1621 gtcactctcg ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt gatgagggcg
     1681 accggatttt ccttggcagc aattcgggag gtgttactcg tgaatacccg attcagctta
     1741 ctgaagaaga cagagaatat ttaaaccagt ttgaattgat tcatatggga ttatatagcc
     1801 atgtcaatca tctattagaa gagttacaaa atgtttctgg aaagatttct tatgattttt
     1861 ctgacgactt tacggaagaa gaggttcagc gggcaattga taaagttgat tttggcttct
     1921 tttctataga agacttttcc gatgaagaag taaagcagtt tttgatgaag catttttgtg
     1981 agcgaaatga tgtattgatt gcaacccgag gagaaaaaac ggctattggt tatgatggag
     2041 caaagtttta tgaagtgaac cccgtgctag aagaaccagt tgatacaatg gcagcgggag
     2101 attctttctt aacagctttc ctactttatt ttctcgaagg ggacgatata gtcaccgcga
     2161 tgaaaaaggg gaatgaattt gctgggaagt cttgtctggt tgacggttcg ttcggctttg
     2221 gtattcatta cgattgagct tgttttaaat tcctgcattt gataacctaa agaaagaaat
     2281 cccggtaaag tggaggttaa aaaaatgctg gatacaaatg cacaattacc attgtactta
     2341 caattaaaag aaatattaaa aaataaaatc aatacagggg agtatcccga gaatacgaaa
     2401 attcccgctg aatcagagct gatagaaagt tatgaggtca gccgaatcac agtgagaaaa
     2461 gcagtagaag aactggtaga agaagggtac ctacttaaaa aacaagggaa aggaactttt
     2521 gtcaaccggc ataaagtttt tcgtaaaatt gaatatatta tgggtttcag tgagagttgt
     2581 aaatctaatg gttttacacc gactagcatt gttttggaac gcaagatcat tccagcaact
     2641 gaagggatgg caaaaaaact cgatatcgct caaggtgatg atgttttata tatccagaga
     2701 aagcgaatgg cggataacat ccccattatg ttagaaaaca attatttttc ttatgaaaga
     2761 tatagtttct taatggagga agagctgact gattctttgt atgaagtact taaagtccaa
     2821 ggtaccttag ccatcaatcc tggggaaact gcacttgaaa ttgtcacagc tgatgaccaa
     2881 attgccaaaa taatggaagt agctgttggg acgccctttt tctttatgga tactttaatc
     2941 aacgatcaaa acaacatacc aattcatgta ggtaatcaat attacctggg agaagtatat
     3001 cgtttttact tatgatttcc tgtttggatt tcaaggagta gattttattt atgaagcaaa
     3061 tattgcggtc aaaaatgatt tttgatggag agcagccacc tttcaaaggt tatattgtaa
     3121 tcaatgaagg gatcattgaa aaggtggaaa acaactggaa ttacagtgat ctattaaatg
     3181 aaaacacgga attaattgct tatgatgatc aattcgttat gccagcaatt catgataatc
     3241 atgtattttt cagtggttat ttggctatga atgctggcat agatttaagc gatacatctt
     3301 cagctaacga ggctgtagag cgtattaaag ctgtaatgaa ccataaaaaa gcagatgaac
     3361 caatttatgc tcatggctgg gatgctgccc tgtggaaaac tgacccatca gaagagctat
     3421 tgaatgaaat aaaccacttt ggtccaatca cagcgatcga caaaaatcgc agccattgtt
     3481 ggatgaatgc tattgctaag cagacttatg gttttacaga aaaagaaacc agtgctgaaa
     3541 gtcgagtatt attaattaag gaaatgctag caaacaaaaa gcttttagca gaggtttacg
     3601 ctgaatttga agcattactg ctttcaaaag gtgttatatc aatgaaagaa atcgtttttg
     3661 atgacgcaga atttctttct aatatacctc agaggaaaat acttagtaac ctatatgtgc
     3721 aggcagtcca agaacctcta cagcatgaac ttctatatga gtataaggaa aaagttttcc
     3781 ctgaaaaggt ccgctttggt ggagtgaaga tcatggtaga tggtgttgtt gcggatagaa
     3841 gtggagatat ttacggtgtt tatcagtcag gagtagcttc acccgagatt gactatgagg
     3901 caattaaagc agaagttgct gcattcaatg aagtagagat tccttgctgt ttgacaacgg
     3961 aaggagataa agcgggttat aaagctgcgt caattcttgt cgaatttgga aagcggttac
     4021 ctgaaggcgt ttttaattcg attagtgatt tggaaatggt tactcaaaag acagcagaaa
     4081 aaatgaacga aggacaagta gtagcagagg tatatccaca aattctcggc ttgaatcaaa
     4141 cgttagaaga ggcttacatg tcaactgtta taaaaaat
//
LOCUS       DARDHD010000187         4176 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000187 DARDHD010000000
VERSION     DARDHD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.190"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..672
                     /locus_tag="SDA06_002462"
     CDS             28..672
                     /locus_tag="SDA06_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4229711.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(728..1231)
                     /locus_tag="SDA06_002463"
     CDS             complement(728..1231)
                     /locus_tag="SDA06_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4229712.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1244..1290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1828..2793
                     /locus_tag="SDA06_002464"
     CDS             1828..2793
                     /locus_tag="SDA06_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4229713.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(2892..4064)
                     /locus_tag="SDA06_002465"
     CDS             complement(2892..4064)
                     /locus_tag="SDA06_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4229714.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4051..>4176)
                     /locus_tag="SDA06_002466"
     CDS             complement(4051..>4176)
                     /locus_tag="SDA06_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4229715.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         1411 a          792 c          749 g         1224 t
ORIGIN      
        1 taaaatgaga caattggagg tatagcgatg ctattaaaaa cacttgtata taagaaaaaa
       61 gatctgacaa ccgttacaga gactagtaca ttagaagaag cattaaaaat tttagaaact
      121 tccggatacc gttgtgtgcc gatcttagat tcttcaggta atattttccg tggaaacatt
      181 tacaaaatgc atatctatcg tcataaagca aatggcggtg atatgacgct tcctgtcact
      241 catcttttga aaaacgctac aaagttcatt tacctggata cttctttctt caaagtattc
      301 tttacgatca aagagcttcc ttatattgct gtccttgaca gtgaaaatca tttttatggt
      361 attttgacac atagtacgtt gcttaatatg ctctctcaat cttggagcgt agaacaagga
      421 agctatgtac tgacaattgc ttccacagga aagcaaggag atttggctgc tattgcaaaa
      481 atcatttcaa aacacagcag cattgccagc tgtattactc ttgatatcga aaaagacgaa
      541 tacattcgac gtacattgat cacgctaccg gctaacactg aagcgaaagt atgtgaaacg
      601 atcgtcaacc acttagagaa aaagaattat aaagtggtag aaattgaaaa tttgcaaaac
      661 caagaagcat aatcaaaaag cgaaaaaaga aaacagctaa tctgctttct tttttcgctt
      721 tttataatta gacacgcttt tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg
      781 ctgaaacagc catgacgata aactcactta atgcaagcgt gccataggtt gtccaaaatg
      841 cttggccacc tgttggaact aacatataag caatcagtcc catgctgatg gtaaagaaga
      901 tcgtgttcaa tactaaacgc atctttacat ttttcacttt gttttgcaat aaagctgtca
      961 ggcctaaaga aagcaatgtt tggccgccac caaaaaccac atccatgata ccaaaaccaa
     1021 agaaggcatt atagacgatg accccgccta aaactcccca aagcaattta cggttaaaga
     1081 caactaaatg attcaaactt tctgaaatac gaaactggat cggccctgag gatacaggcg
     1141 caactaaaat agttaacgct agataaagag ccatgatgat gccatttaca acgattacgg
     1201 ataaccgttg tctgcttttc gttttttgca ttttctctcc tcttttctcc tagttttttt
     1261 aacgcgggat ggttgatgaa ccgcggttat gtgaaacaaa caataattat aacagacttt
     1321 tcatttattt caatccgtct tatctgcttt taaaaaagca aaagcttaaa caatgtaaac
     1381 gtttgctaaa aatcgtggga tatggtataa agtaagtgta aggggtcata agaattccca
     1441 gtaaagcaga cgaagtagga tctgcaagaa cagaattgtt gtgaaactta aataaataaa
     1501 aaaggatgtg ggaatttgag caacctctta ctagagaacg gtctagcaag cagctgaaga
     1561 tctaagtgga catgccgaac cttggaactt ctccttacgt aagtaatgga tcatgcgctt
     1621 gctaggcttt tttgtgtttt tcggtcttcg gacctttttc attttcctag acttctgcta
     1681 taataaccaa gaattaattt tgtaacagat ttttttgaaa aaacaagttt ctttcattca
     1741 aaaaatagaa aaagcttgct aattattggt taaccagcaa acaggagtat taaaatatct
     1801 tgttttattg aatagcagga ggaaatcatg aaattccatt atcatatatt gatcaatcca
     1861 tcggcaggca gcggaaatgg ctataaagta gcagaaagaa ttttaccggt attaaaaaat
     1921 aagcatattg actatacact tcattatagt gaatacaaag gacacgatgc tgagattgct
     1981 gaaactttgg caaaagaaac attgattcct tggatagaag aagaacagaa aacaatcgat
     2041 actttccccc ttttgatcat cgtaggaggt gacggcacct tacatcaggt acttgatact
     2101 ttctatcaga tggaagtaga attcccagtt gcttatattc cagcaggttc tggagatgat
     2161 tttgctcgag gggcaggcct tccaaaaaat cccaaaaaag gacttcagct gattttagca
     2221 gctcaatcac cagaaaaagt ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa
     2281 ggaatagctg ttaataattt cggtatagga ttagatgcag ctatcgtcca tgcgacaaat
     2341 cattcttcca caaaaaagcg attgaacaaa tacaacttag gatcattttc gtatttattc
     2401 tctattctcc gtgctttatt cactcaaaaa ggatttccaa ttctggtaga tgcaggagga
     2461 aaacaattaa gtttttcaaa cgcattttta tgtacagcaa cgaagcatcc tttttttggc
     2521 ggaggaatcg cgattgttcc aactgctgat gcgtctaaac cagtcattga ttttgtcgtg
     2581 gtcgaacgaa tcaatttctt taaaatcttg tggctcattc ttttacttat acagaaaaaa
     2641 caattgaaat cgaaatattt ccatcactac acaacaaaca gattacgtat cgtttcaaca
     2701 acaccgcaac atggtcaaga agatggagaa gaaatggaaa aacgaccttt tgatatcaca
     2761 ataaccacaa ctaaacagtt gttttggtgt taataaaaga ggttttgtca gaagctacat
     2821 ctggaacacc gtttattcgg aaaaaaggac tgtgacacga cttttgtcac agtccttttt
     2881 ttactatgca gttaattctt tgcccaattg ggttgccatc gatagtttct ttctttaccc
     2941 aaaataaatt ggatcattga aaacaccgca gcaaaagcaa ctaagacaca accattgaac
     3001 caaaaagatt ctgcaaatgt ttgattgtaa ctgagtgttt cactgatcaa gttattattt
     3061 tgaacaaacc aatatgttgg tgtgaatgcc gctattttgc taaccatttc tggcaagtat
     3121 tctgctggta caaacacccc accgataaag caactgcctg tgataaaaat atctttgatc
     3181 ccaccgctgg tttctgagtt ttttaccaat gaagtgatac aagccgaaaa gctaatgatc
     3241 ggcaataaaa acaagaaagt atttaaaata tgataccata ccgttatatc ccacgaggtg
     3301 tggatcacta acagaaccat tagaagtaag aagccactga acaacagcag cgagtaacta
     3361 atcaaactaa aggttacttt gcgattcaag tttcgtttgg taatgggcgc actctggttt
     3421 cgtttactga tctctttgcg attaaatgct agattgatga cgctgaaacc gctaaaaatg
     3481 gatagaaaca acccataaga caagaaatta aaggttgtgc ccgtcaacag ctttttcatc
     3541 cgctgactat atcccgcact tagctcaact tctccagtaa ccgagagtgc catttgtgtc
     3601 tgttccatag ctgcatcgat tgttttatct tcttcttgaa acagaatcag tgtttgcaaa
     3661 aaggtgttca cttgctgtgt aacaactgtt ttagaaaagg catctgggcg actctgtatc
     3721 ttgatcgtcg gcatctttcc tacttcgact tgttcggtaa agtctgcagg cagatgtaag
     3781 ataatgttta cacgattaaa ataaagagca tcatcgattt ctttttgtga ggttccatcc
     3841 aatgtaacga ctgtttcctt gtcattcaaa tatttacgaa ggccatcgac tacaggcgac
     3901 tcttcttcac taatcaaggc gattttcgca tcttgcagag aggtattttc aacatcaata
     3961 ttttgcgcaa aaaagaatgt ggcaacaaca gtgataatta cacctatcag taaattcgat
     4021 ttatttgctt taagcaatgt ccaaatcgtt ttataaagcg tcataacgca cccctctttc
     4081 catccaagca tttcctaacg caaaaaggaa aataaatccg atcaaccata gaagattggt
     4141 ataaaaacta gccaaatcag tgtagaaata aagttt
//
LOCUS       DARDHD010000188         4154 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000188 DARDHD010000000
VERSION     DARDHD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.191"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..211)
                     /locus_tag="SDA06_002467"
     CDS             complement(<1..211)
                     /locus_tag="SDA06_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEG4229716.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSGMIDK"
     gene            complement(218..652)
                     /locus_tag="SDA06_002468"
     CDS             complement(218..652)
                     /locus_tag="SDA06_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229717.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            800..1941
                     /locus_tag="SDA06_002469"
                     /pseudo
     CDS             800..1941
                     /locus_tag="SDA06_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2067..3146)
                     /locus_tag="SDA06_002470"
     CDS             complement(2067..3146)
                     /locus_tag="SDA06_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4229718.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3249..3689)
                     /locus_tag="SDA06_002471"
     CDS             complement(3249..3689)
                     /locus_tag="SDA06_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4229719.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3746..>4154)
                     /locus_tag="SDA06_002472"
     CDS             complement(3746..>4154)
                     /locus_tag="SDA06_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4229720.1"
                     /translation="KKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPEQ
                     LFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLSL
                     EETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         1517 a          766 c          710 g         1161 t
ORIGIN      
        1 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
       61 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
      121 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
      181 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
      241 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
      301 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
      361 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
      421 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
      481 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
      541 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
      601 tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg
      661 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
      721 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
      781 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
      841 aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg
      901 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
      961 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
     1021 ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
     1081 cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
     1141 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
     1201 atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga
     1261 gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
     1321 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
     1381 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa
     1441 acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg
     1501 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     1561 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
     1621 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
     1681 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
     1741 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
     1801 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
     1861 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
     1921 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
     1981 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
     2041 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
     2101 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
     2161 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
     2221 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
     2281 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
     2341 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
     2401 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
     2461 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
     2521 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
     2581 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
     2641 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
     2701 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
     2761 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
     2821 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
     2881 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
     2941 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
     3001 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
     3061 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
     3121 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
     3181 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
     3241 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
     3301 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
     3361 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
     3421 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
     3481 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
     3541 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
     3601 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
     3661 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
     3721 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
     3781 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
     3841 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
     3901 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
     3961 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
     4021 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
     4081 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
     4141 aatacatttt tttc
//
LOCUS       DARDHD010000189         4124 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000189 DARDHD010000000
VERSION     DARDHD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.192"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..125
                     /locus_tag="SDA06_002473"
     CDS             <1..125
                     /locus_tag="SDA06_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4229721.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="SDA06_002474"
     CDS             130..2259
                     /locus_tag="SDA06_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4229722.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="SDA06_002475"
     CDS             2256..3263
                     /locus_tag="SDA06_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4229723.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4124
                     /locus_tag="SDA06_002476"
     CDS             3280..>4124
                     /locus_tag="SDA06_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4229724.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTT"
BASE COUNT         1355 a          669 c          942 g         1158 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 actctgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caac
//
LOCUS       DARDHD010000190         4103 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000190 DARDHD010000000
VERSION     DARDHD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.193"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..544)
                     /locus_tag="SDA06_002477"
     CDS             complement(65..544)
                     /locus_tag="SDA06_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG4229725.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            903..1685
                     /gene="rpsB"
                     /locus_tag="SDA06_002478"
     CDS             903..1685
                     /gene="rpsB"
                     /locus_tag="SDA06_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG4229726.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1784..2665
                     /locus_tag="SDA06_002479"
     CDS             1784..2665
                     /locus_tag="SDA06_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG4229727.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2801..3523
                     /locus_tag="SDA06_002480"
     CDS             2801..3523
                     /locus_tag="SDA06_002480"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG4229728.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3526..4083
                     /gene="frr"
                     /locus_tag="SDA06_002481"
     CDS             3526..4083
                     /gene="frr"
                     /locus_tag="SDA06_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG4229729.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1471 a          725 c          887 g         1020 t
ORIGIN      
        1 attcaataag gaaaatattg aaaataaaaa aacacgcatt ccatattagg aatacgtgct
       61 ctgattaaaa aagtgatact tcagttggat ggtgaagaaa ttcttctact cgttttgcgt
      121 cctcctcatt ttccgagatg accatgatcg tatcgaatcc agcaattgta ctgacaatag
      181 agggaggttt gatctcatct aaaacagatg caaataaatg agcattgtct ggcaaggtat
      241 tgacgatcgt caaaaagtgg actcgttcga ctttcaatac aatgtcttca atcatttgaa
      301 ccagccgatc ctcttcctca gtcctagtct caggagttcc tttttcgcga aacaatacga
      361 agcgggaaac gccattttca tcaggtgcct tcacgatttt tagatcacgg atatctcgag
      421 aaatcgttgc ctgcgtggcg gtaacgccat aatgcccaag tttgtttaat agttcttctt
      481 gcgtacggat cgggaattct tcaatgactt gtttaatcag caaatgtctt tgagctttgc
      541 gcattttctt tctcctttac gaaagatttc acatgtatca taacaaaaaa aagaaggatt
      601 gcccaataat attcgtcttt tatacctaaa attattttat ggaaaaagga taataaagaa
      661 acaaagagaa atctactgtt tttcttgaat tctttttatc tttcaggtaa actattgaat
      721 gttgagcaga aaagtatatt tgctcttgcg atacggatct tttgtctgta taatagctga
      781 gggtgcttta tgcatcagca aatccacatt cttattggat tgtcggtagg tgcttgaaaa
      841 agtttgccgg caaggtagaa gataagaaga ggaaaaataa aaccaaatcg gaggaaacaa
      901 acatggcagt aatttctatg aaacaattac tagaagccgg cgtacacttt ggtcaccaaa
      961 ctcgtcgctg gaatccaaaa atgaagaaat atatcttcac agaaagaaac ggaatctaca
     1021 tcattgactt gcaaaaaaca gttaaattag tagatgcagc ttatgattac atgaagagcg
     1081 ttgctgaaga aggcggcgta gcattattcg taggtacaaa aaaacaagca caagaagcaa
     1141 tcaaagaaga agcaactcgt gcaggacaat acttcgttaa ccatcgttgg ttaggcggaa
     1201 cattgacaaa ctgggatact atccaaaaac gtatcagccg tttgaaacaa atcaatgcaa
     1261 tggaagaaga tggaactttt gaagttctac ctaaaaaaga agttgctggt ttgaacaaag
     1321 aacgtgaacg tcttgaaaaa ttcttaggcg gtatcgctga tatgccaaga atcccagatg
     1381 taatgtacat cgttgaccca cgtaaagaac gtattgctgt tcaagaagca cacaaattaa
     1441 atatcccaat cgtagctatg gttgatacaa actgcgatcc agatgaaatc gacgtagtaa
     1501 tcccttcaaa cgacgatgca attcgtgccg ttaaattgat tacttcaaaa atggctgatg
     1561 ctttcatcga aggaaaccaa ggtgaagatc aagtagttga agaagatttc acagaagaaa
     1621 acacagcaac atcaatcgaa gaaatcgtag atgttgttga aggcgacaat tcttcagcag
     1681 aataattttg aatgatggag ctgtttcaaa ggctgggcga caagcctcac tctcctttga
     1741 agcagctttt tttaaaaaga aaaacacaca ggaggagtta aacatggcag acgttacagc
     1801 taaaatggta aaagaactac gcgaaatgac tggcgtagga atgatggatg cgaaaaaagc
     1861 attagttgaa gtagaaggcg acatggaaaa agcagttgat cttttacgtg aaaaaggaat
     1921 ggcaaaggct gcgaagaaaa atgaccgcat tgcagctgaa ggattggctt cagttgctgt
     1981 gaaaggtaac actgcagcaa tcgttgaagt aaactcagaa acagactttg tttctaaaaa
     2041 cgaaatgttc caagatttag taaaagaaat tgcagaactt gttgctgaaa acaaaccagc
     2101 agacatggaa gctgcaatga aaatcaaaac agataaaggt acaattgaat ctgacttgat
     2161 cgaagcaact caagttatcg gagaaaaaat cagcttccgt cgctttgaag ttgtagaaaa
     2221 agacgacaat gctgcatttg gtggatactt gcatatgggc ggacgtattg ccgtattgac
     2281 tgttctagat ggaacaactg acgaaacagt tgcaagagac gtagctatgc acgtagcagc
     2341 tatcaatcct cgttatgtaa acgaatcaca aattccagaa gctgaattag aacatgaaaa
     2401 aactgttctt acagaacaag cattgaacga aggcaaacca gcgaatatcg ttgaaaaaat
     2461 ggtagaagga cgcttgaaga aattcaaagc agaaatcgct ttggtggacc aaccattcgt
     2521 taaagatcct gatatgactg ttgaaaaata cgttgcatca aaaggcgcaa ctgttaaaac
     2581 attcgtacgt ttcgaagttg gcgaaggtat cgaaaaacgc gaagataact ttgtagaaga
     2641 agttatgagc caagtgaaaa aataattctg ttgaataaca gacgggaacg cacggtttgt
     2701 gcgctccctt tttttaagca aaacaagaaa aaccagtctt agagtgtcga aatcatctaa
     2761 gtatggtaaa atggcaatag aaaatattgg aggaatgaac atggtcacac cgaaatatca
     2821 gcgtgtagtc ttaaaattga gtggagaagc tttagctggg gacgcaggat ttggaatcaa
     2881 accaccagta atcaaagaaa tcatccaaga aatcaaagaa gttcacgagc tgggcgttga
     2941 aatggcaatc gttgtgggcg gcggaaatat ctggcgcgga caaatcggag cacaaatggg
     3001 catggaacgt gctcaagctg attacatggg tatgttagct acagttatga atgccttagc
     3061 tttacaggat acgttggaaa acgtgggtgt accgacacgc gtgcaaactt cgattgaaat
     3121 gcgtcaaatc gcagagccat atattcgccg taaagcagaa cgtcatttag aaaaaggacg
     3181 gatcgttatc tttgcaggcg gtaccggcaa cccgtacttc tcgacagaca caacagcagc
     3241 attacgcgcg gcagaaatcg gcgcagatgt tatcctgatg gctaaaaaca atgtagacgg
     3301 tgtttattct gctgatccga aagtagatgc aaatgcagta aaatttgaag aactgactca
     3361 tctggaagtt attgccaaag gattacaagt catggattca actgcaagtt cactaagcat
     3421 ggataatgat attccactgc ttgtcttcaa tttaaatgaa cacggaaata ttcgccgtgc
     3481 aatcttaggc gaaaatatcg gaacaactgt aagggggaaa taaaaatggc agatgcaatt
     3541 atgacagaag caaaagaaaa aatgaaaaaa gcagcacaaa atcttcaacg tgaacttggg
     3601 caaatccgcg caggccgtgc gaacgctagc cttttggacc gtattactgt gaactactac
     3661 ggtgcgccaa caccattgaa ccaaatggca tcaatccaaa ttccagaagc acgtgtttta
     3721 atgatcacac cttttgacaa gagcattctt caagatgttg aaaaagctat tatggcaagt
     3781 gatatcggaa tcagtccaac caatgatgga aatgtcattc gcttagttat cccgcaactg
     3841 acagaagaac gcagaaaaga attagctaaa gatgttaaaa aagaagcaga aaatgcaaaa
     3901 atcgcgattc gtaacatccg tcgtgatgcg atcgatgaat ataaaaaaca acaaaaaaat
     3961 ggtgatatta ccgaagatga tttgcgcgga ttagaaaaag acgtacaaac attgacagat
     4021 aacagcatca aagaagtaga taagatcgct gctgataaag aaaaagaatt attggaagtt
     4081 taataaaatc ataaaaacag agg
//
LOCUS       DARDHD010000191         4031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000191 DARDHD010000000
VERSION     DARDHD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.194"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(142..1647)
                     /locus_tag="SDA06_002482"
     CDS             complement(142..1647)
                     /locus_tag="SDA06_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4229730.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1644..2330)
                     /locus_tag="SDA06_002483"
     CDS             complement(1644..2330)
                     /locus_tag="SDA06_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4229731.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2523..3944)
                     /gene="gndA"
                     /locus_tag="SDA06_002484"
     CDS             complement(2523..3944)
                     /gene="gndA"
                     /locus_tag="SDA06_002484"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG4229732.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1109 a          854 c          690 g         1378 t
ORIGIN      
        1 tacttacatt taattaacgg atgttattct atttaattgt tcgctagttt ttttataatg
       61 acatccctga atttctcact tgcttgtatt ttgtacctta ttctttgttg gatatataaa
      121 aaaacaagta aagcacatgc tttacttgtc ttctgccttt gcgataggaa taaagattct
      181 aaaaattgtc ccttgcccta ctacactttc tgctaagata cgtcctttat agttttcaac
      241 cagttgttta gcgatagaca accccagacc atttccaccc ttcgttctag ctcttgcttt
      301 atccactcga taaaaacgat tgaagatctt attaaggtct tctttgggta ttccttcgcc
      361 aaagtcctga atcgcgatct caaattcatt cagagtggaa gaaatagaga tatggacttc
      421 ttttcgtgta gttgaatact ttactgcgtt atccaatatg ataatgatta attgttcaaa
      481 gtgattacga taaatttgca gggtcacttc ttttgtcaaa tcatcatcta aagtgatcac
      541 atagtctgga tataatatct tgaaattatt ataaacttga taggtgactt cttttccttt
      601 agttgtttca ttcgcataat gaacatctac ttgttctgct cttgataagt ctagcatctc
      661 ttgaactaag cttttcatac ggctgatttc ttgcatgctg gcgcttaatg attcttctaa
      721 tatctcagga tcatctttcc cccaacgatt tagcagacta agatgccctt caataattgc
      781 aacaggtgtc cttaactcat gagatacgtc ttccacgaat tgctcttgtt gttcaatata
      841 caaacgcata cggtcaagca tctcattgaa tatttcagct aaatctgcca attcatcatt
      901 tgtgtcgatt tcaggcatgt ggatatctga ttgcggatct ttacgtatag tatccattgt
      961 atctctgagt accttgagtg gttttaagaa ataagaagat aaaaagaaac ctaaaatact
     1021 acttaagatc aaagaaacaa cttctaaaac aataagagtc aataatagct tatttcgaat
     1081 atcatagaaa gaagataatt catagaagct ttgcgcatag ccgatctttt ctcttgtacc
     1141 tttagaatat ataggctgga tagataaaaa gccagttttt ccttctagtg taacgatcgt
     1201 tggaacttta cgtgtagtct gaatcaggtt tcgactgttt tcatgggttt taaaaatcaa
     1261 gttctcgtcc aaatcataaa cataaagatc cattgatggc tgccctagct cagctataaa
     1321 ggaatcgatc ttcattaagt ttccttcgac cagtgaagta tgatctacga cctcatctcc
     1381 gtcatttgtc gctttcgtca gattacgata cgtatttact aatgtcagtt cttcatccga
     1441 gttagccaaa cgagaagcta cttctgaaat cgtacgctct acattgtttc tttcttttgc
     1501 cacaatcaga ttgatagatg atttataggt aatgacagcg aaaattgtaa acacaacgaa
     1561 tatgaagaaa gaactcgcaa aggcccactt gattgtcaag gagggccctt tcagttctct
     1621 tttttccgat tttttcggct ttatcacgag cgcatcacat aacctgttcc gcgtacagtt
     1681 tggatatagc tttcctcccc gggaacgtca attttgttgc gaagataacg aatatatacg
     1741 tccactacgt ttgtttcaac ttctgtttcg tatccccaca ctttatttag taaaacgtca
     1801 cgtgctaata ccacgttcac gttttccatt aaagtaagca gcaactcgta ttctcttttt
     1861 gttagttcaa ttacttcatt tccgcgacgt acgacacggt tttctttttc gatagtcaaa
     1921 tcgcgataag taatagtagt ttgtttagcc acatttttat cgccttcaat atcgattcga
     1981 cgcaacaatg cacgaagacg tgctaataat tcttcgatag caaatggttt gacgatgtag
     2041 tcatctgctc cgtggtcaag tccagataca cgatcgatca cagaatctct tgcagtcatc
     2101 ataatgattg gtgtattttt gacttggcgt acacgtctgc agacttctaa accgttcaat
     2161 tctgggagca tcagatccaa caatatggca tcccactcat tatttaatgc cgcatctaaa
     2221 ccagttcggc cgttgtagtg gacttctgcg ttataccctt catgcttcaa ctctagctcg
     2281 acaaatcgtg ccaagttctt ttcatcttca atgattagaa tattgctcat ttattatttt
     2341 atcctttcta ttctactggc tttttaaaaa ctctatcatt ataccgataa tatgcttaaa
     2401 aatctatagt cattttatca tggatagcaa ttttaaaaaa gagctgaagt ctactcataa
     2461 caatcgactt cagctctttc gtcctctttt tattttctca tttcctaaca gccgaaagac
     2521 tattattctt catggtacca tgagtaatgg aagattcctt ctttgtcgac acgctcatag
     2581 gtatgtgcgc caaagtaatc gcgttgggct tggattatat ttgcaggcag acgttctgct
     2641 ctgtatgaat cgaagtaagc gatggctgat gagaaagtcg gtacaggtac gccagcttgg
     2701 acagccaagg caacgacgtc tctgactgat tgctgatatt ttttcgtgat ttcaacgaag
     2761 taatcatcta gcaataaatt ttcgatctca ggatttttgt cataggcatc cgtaattttt
     2821 tgcaagaatc gagctcggat gatacatcca gcgcgccaga tttttgcgat ttcaccaaat
     2881 ggcaagtccc aatcaaattc ttttgaagcg acacgtaatt gagcaaatcc ttgggcataa
     2941 ctcatgattt tactgaaata caacgcttct cggatttttt cgaccaattc ttttttgtct
     3001 cccgtgaact caaaatcgtt cgtacgagac aaaatcttgc ttgcttgtac gcgttcttct
     3061 ttataagcag aaatatagcg ggcaaataca gattcagtga tcaatggaag tggcacacct
     3121 aagtcaagcg cgctttggct tgtccattta cctgttcctt tattcccagc tgcatcaagg
     3181 atcacatcta ctactggttt acctgttcct tcatcgtctt tacgtgtcag aatgtcagca
     3241 gtgatttcta tcaaatagct gtctaattcg ccttgattcc attctttaaa gatatcagcc
     3301 atttcttcta ctgaaaggtt taaaatattc ttcattaagt cataagattc agcaatcagt
     3361 tgcatatctc catactcaat accgttgtgt accattttca cataatgtcc ggctccattt
     3421 gggccaatat atgtgacaca tggtgctccg tcttctgctt ttgcagaaat ttgctctaag
     3481 attggtgcaa ctagcgcata ggcttctttt tgtccaccag gcatgattga aggtcctttt
     3541 aaagctcctt cttcaccacc ggaaacgcct gtaccgataa agttgatgcc agagttggct
     3601 aactcttcat tacgacgcat cgtatcttta aagaatgtat ttcctccgtc aatcaaaata
     3661 tcgcctttat caaggtgagg aagtaactct tggattgttg catctgttgc cggtcctgct
     3721 ttgaccatta gcaagatccg acgtggtttc tcgatagatt ctacaaattc ttcgattgta
     3781 tatgttgctt tgaaattttt atctgggtgt tcttctacta cagcggttgt tttagcccct
     3841 gtacgattga ataaagccac tgaatagccg cggctttcta tgttcaaggc tagattttta
     3901 cccataacgg ccataccgac aacgccgatt tgttgttttg acatatgaac tcctcctaca
     3961 tacgggattt ctccctaatc tcttacatta tagcttaaca cggctacatt gcaaactaaa
     4021 gaacaacata t
//
LOCUS       DARDHD010000192         4029 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000192 DARDHD010000000
VERSION     DARDHD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.195"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            37..1338
                     /locus_tag="SDA06_002485"
     CDS             37..1338
                     /locus_tag="SDA06_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229733.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1365..1625
                     /locus_tag="SDA06_002486"
     CDS             1365..1625
                     /locus_tag="SDA06_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4229734.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2624..3859)
                     /locus_tag="SDA06_002487"
     CDS             complement(2624..3859)
                     /locus_tag="SDA06_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4229735.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1145 a          653 c          846 g         1385 t
ORIGIN      
        1 gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat gaaatgggtc
       61 gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt attagcaatt
      121 cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt tatggtcttt
      181 atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc aacgattgtt
      241 ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt tatgggttcg
      301 gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc tttaggaacg
      361 ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc tcttggctgg
      421 gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt atctatgatt
      481 tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa aatgccagaa
      541 caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc agtcatttta
      601 acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc cttattatcg
      661 ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt actaccaatg
      721 tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt aggggctgtt
      781 gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc agatggtaca
      841 gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac ggtaacaatt
      901 ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg taaatcaaaa
      961 ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat caatgagccc
     1021 ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt caacttagtt
     1081 cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt caatagattt
     1141 actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc agcaggaaat
     1201 gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat tatctattat
     1261 ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg tggagaaaac
     1321 gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat tggctgattt
     1381 tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat atgctagcta
     1441 catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg atcgtgattc
     1501 ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca tatgtacttt
     1561 gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc acatcgaagc
     1621 tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat aagaagaaat
     1681 ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt ttggttgaac
     1741 ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga ctgtgacaag
     1801 gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt tttggcagaa
     1861 agaaggagga aaaaatggaa attttatcag caatggattt agaattagta gatctactac
     1921 caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag gggctgcagt
     1981 atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg aaacgtgtat
     2041 agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt agtgtatcgt
     2101 tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt gctggcagtt
     2161 tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg gaggttgatt
     2221 atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg gactagcggc
     2281 acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga aaatgtattt
     2341 gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc agagaaaggt
     2401 gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt ggatagcgtc
     2461 agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta agtaactcta
     2521 acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc gaatagcgaa
     2581 attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt gggtggttgc
     2641 tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga aaaacggctt
     2701 gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca
     2761 gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt acacaatagt
     2821 caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg ccggattcac
     2881 aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg aattcatgca
     2941 aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat tgaatgtcat
     3001 caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt aagaagaaca
     3061 aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag ataatacgtt
     3121 ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag gaagtactga
     3181 gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct tcttccaaaa
     3241 atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa cttgtttttg
     3301 gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc catcgggtat
     3361 ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg aagaaacgta
     3421 actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt
     3481 taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc acattcgaac
     3541 ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta gtgatacgag
     3601 aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca aattttatga
     3661 tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca aagccacttt
     3721 ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa taacggtctt
     3781 tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat actgcaggag
     3841 caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc ttcgtttctt
     3901 actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa tattaaactg
     3961 tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga aactccaatt
     4021 ttcaaaaaa
//
LOCUS       DARDHD010000193         4016 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000193 DARDHD010000000
VERSION     DARDHD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.196"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(232..381)
                     /locus_tag="SDA06_002488"
     CDS             complement(232..381)
                     /locus_tag="SDA06_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229736.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="SDA06_002489"
     CDS             843..1055
                     /locus_tag="SDA06_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229737.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="SDA06_002490"
     CDS             complement(1333..3132)
                     /locus_tag="SDA06_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4229738.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="SDA06_002491"
     CDS             complement(3256..3852)
                     /locus_tag="SDA06_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4229739.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          688 g         1174 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataac
//
LOCUS       DARDHD010000194         3980 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000194 DARDHD010000000
VERSION     DARDHD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.197"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..715
                     /locus_tag="SDA06_002492"
     CDS             56..715
                     /locus_tag="SDA06_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4229740.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="SDA06_002493"
     CDS             800..1705
                     /locus_tag="SDA06_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4229741.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="SDA06_002494"
     CDS             complement(1707..2339)
                     /locus_tag="SDA06_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4229742.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="SDA06_002495"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="SDA06_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG4229743.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="SDA06_002496"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="SDA06_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG4229744.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="SDA06_002497"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="SDA06_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG4229745.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
BASE COUNT         1338 a          700 c          827 g         1115 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata
//
LOCUS       DARDHD010000195         3925 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000195 DARDHD010000000
VERSION     DARDHD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.198"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(195..488)
                     /gene="rpmA"
                     /locus_tag="SDA06_002498"
     CDS             complement(195..488)
                     /gene="rpmA"
                     /locus_tag="SDA06_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG4229746.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(501..845)
                     /locus_tag="SDA06_002499"
     CDS             complement(501..845)
                     /locus_tag="SDA06_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG4229747.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(872..1180)
                     /gene="rplU"
                     /locus_tag="SDA06_002500"
     CDS             complement(872..1180)
                     /gene="rplU"
                     /locus_tag="SDA06_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG4229748.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1200..1276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1462..2103
                     /locus_tag="SDA06_002501"
     CDS             1462..2103
                     /locus_tag="SDA06_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4229749.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2165..3796)
                     /locus_tag="SDA06_002502"
     CDS             complement(2165..3796)
                     /locus_tag="SDA06_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG4229750.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1221 a          857 c          680 g         1167 t
ORIGIN      
        1 ctgatagcaa acaatatagc ttccatcaat tgaaaacgat agttatcaaa acatcctgat
       61 tccaaattgg caaaaaaata ttaagtgtaa aaagacaaag taaacttcgt agcctccact
      121 acaactttta tttttataaa caaaaaaggc tgtgacaaaa gtcttgtcac aacctcttta
      181 atcataagat tcaattaagc ttcttgagct actggataaa cagacacttg ttttttgtta
      241 cggcctttac gttcgaaacg tactacgcca tcaacttttg caaataaagt gtcgtctcca
      301 ccgataccca cgtttacacc tggatagatt tttgtaccgc gttgacggta aagaattgat
      361 ccacctgtaa ctgtttgtcc atcagcacgt ttagcaccta gacgttttga ttctgagtca
      421 cggccgttag atgtagaacc gccacctttt ttgtgggcaa ataattgtag attcatagat
      481 aacaacatgg tctgcacctc ctatttatca ttaattgttt taatttggat atattctgag
      541 ttttcagctt ctatcgattg taatcctaat aaaagatttt ctaatagaat ctcagcaata
      601 ttgttttgtt cttgtgtcat tcctgaagtg acttcggtat aaagataacc gccttcttct
      661 tcattcattt caacaatcgg ctcaaaaccg gctaatgagg caatcccgtt gacagtgctg
      721 atcgctaacg cagaaacacc cgcacaaacg atatcacttc cataaggacc agcatcagca
      781 tgcccggtca aagtgaatga aacgatctga cctgcgtcat ttcgcttaaa tgttcctttg
      841 atcatatata ggccttcttt ctcaaacgct attaagcgtt gatcgcgtcg ataaccactt
      901 ttgtatatgg ttgacgatga ccttgtttac ggtgtgaatg ttttttaggt ttgtatttga
      961 aagtaacgac tttcttttgt ttgccgtgtt tttcaacagt accttcaaca gttgcacctg
     1021 caacagttgg agcgcctact tttgtagatt ctccgcctac taagataact tcgtcaaaaa
     1081 caactttttc tcctgcttca acgtcaagtt tttcaacata gattgcttgt cccacttcaa
     1141 cttttacttg tttaccacca gttttaataa ttgcgtacat gctataaagc acctccttct
     1201 gatatactta gactcgccat ccgaagtgac agcatatgct gtgcttatta cttgttctga
     1261 gcggttgtag ctgtggagtc cacaattaca acagtactag cttaccagaa cagcaaccgt
     1321 tagtcaacta aatttaaaat caaaaagtaa aacaaaaatt caagcaatac ttctgctttt
     1381 ctatcctcac agttctacca aataaaatag tcgcattgtg tttcttttat tcttataata
     1441 aaagaaaaag gaggagcata aatggaatac ccaaaagaac tgatacaaga agcttcttta
     1501 agaatgaacg gccgtcctgt agaaggattt atcgctgggc aaggacctct tcatcctaaa
     1561 ctgatgctcg tgggagaagc cccagggaaa acagagattg atacgcatgt tccttttagc
     1621 ggtcaagctg gaaaagaatt gatgcaggca ctttcttcaa ctggcttaac acgagaagag
     1681 gtgtatatca ccagtgctgt aaggagccgc ccgtatcgcg tgactcatcg gatcaataaa
     1741 cggacacaac agtcagaaac tgtttatcca aatcggacac ctactcgttc cgaagtattt
     1801 gcgcatgcac caatattgga ttacgaactg atgaaggtcc agccaaaatt aattgcgaca
     1861 cttggaaaca ttggtcttca acgtttactt ggcaaagaat atacggttac caaaaatcat
     1921 ggtcaactct atacagggcc tgtcttacaa ttaacagaac aaaaggatgc ctacgaatgg
     1981 tcagaaaaaa actatcgaat tcttccactt tttcatccag cagctatttt ctacaatcgc
     2041 tcccttgtac ctgttatcca tcaagactgg caagtactag cttcactttt acagcaatat
     2101 taaaaagaac agacaagacc ataaaatctt cagtttacag tcttgtctgt tctttttcat
     2161 tgttttaatg gctaattgca gtatatttcg catgtaaagc tgtcaacgca tgagcaactg
     2221 tttgatgttg atgcgtgaat gtatgttccc acaagtgaga aagttgtttt tctacttctg
     2281 gctgcaagga agtatagata atcgtttgtc ctctttgctc tgctttttct tgaaattcat
     2341 caaaaactgc acgtgccgtc aaatcaatac ttggtacgcc tcgcatggag aaaatgacac
     2401 catcgtgatg atttagttct tccaatttat ttttgagttt ttctgaagac ataaaaaaca
     2461 gtggaccact aatatacact actgtccagt tattcagctt ttccgtttct ggcagcccca
     2521 ttctttgcca atcgatcgtt tctaatgaaa tcgtgatagc agcgcttttg acaacaaaaa
     2581 atacacagcc gccgatcacc ccgatcacga tagcaatact taaatcaaag ataactgtac
     2641 aaatcatcgt aaggataaac aatagaactg ctgaccaata tttattagta aatagctcct
     2701 tgatcgtttc ccattcattc atacgccaag ccgtcacaat caaaacacct gcaagtgctg
     2761 gcattggaat ctgtgacatc actggcgcaa aaacaagcat ggagagcagt aagacaactg
     2821 catgtatcat accagctaat ctagtttgtg caccagattt gatggccacg cttgtacggg
     2881 cgatggccgc agtagcagga atcccgccaa agaaaggaag caacagattt ccaattcctt
     2941 gcgccattaa ttcttgattg ctatctaagg gcttatttgc cattcggcca gccgaagcac
     3001 cacatagcaa gctttcaatc attcctaata aagcaatgct aatagctgga actgccactt
     3061 gttgcaaggc agaaaagcta aaatctccca tattcaaacg attactactg atcaatgttt
     3121 gtggaatctt acccacagtt gctactggta aatggaacaa tagcataact gctgttgcca
     3181 aaatgatagc cagcagcgaa ctaggcattc gtttcgccca tttttttgga tagataaaca
     3241 tccctaaaat gactagccct cccatgatca aagaggctgt gtttatcgga aatcctatcg
     3301 tttgataact acttagtttt tcaataacgt ttgtaccttc cgagtgaaca ccaaataaat
     3361 tatcgatctg acctaatgca ataatgatag caatacctga agtaaacccc gtaattacgg
     3421 gtgctgggat aaaagaagtc agtgttccta atcggaaaag tcctgctgca agtaaaaata
     3481 gacccgctaa aaaagtagca agcaatacac cttgcattcc ctttgttgcc gcaatagaca
     3541 tcaagatagc tgccatcgca cctgtcggac ctgatatttg ataaaaaccg cctgataaac
     3601 cgccaataac gataccggca atgatagccg taatcaaacc tgcggccgca tcagctccgc
     3661 ttgatacccc aaaagccaag gctaaaggca atgctacagc agctactgtg atcccagcca
     3721 aaagatcttt ctgaaatctt gaaagatcat aacctgaaaa ttcttttttt agtgaggaaa
     3781 cataattttt gaacacctat ctaactcctt tttcatgaaa ttttctaaaa taccattcat
     3841 tagaatatca tatttcatga gataaagtaa tatttttcac aaagtttcct gttagataaa
     3901 ttcttatttt ttctatattt ataag
//
LOCUS       DARDHD010000196         3895 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000196 DARDHD010000000
VERSION     DARDHD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.199"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SDA06_002503"
     CDS             complement(<1..1256)
                     /locus_tag="SDA06_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4229751.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SDA06_002504"
     CDS             complement(1270..2487)
                     /locus_tag="SDA06_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4229752.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3895)
                     /locus_tag="SDA06_002505"
     CDS             complement(2477..>3895)
                     /locus_tag="SDA06_002505"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4229753.1"
                     /translation="VSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLAD
                     QAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKL
                     NDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRF
                     FSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNL
                     RNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFA
                     FLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTS
                     IPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAH
                     LATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISS
                     LQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1070 a          839 c          659 g         1327 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttac
//
LOCUS       DARDHD010000197         3875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000197 DARDHD010000000
VERSION     DARDHD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.200"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SDA06_002506"
     CDS             158..2257
                     /locus_tag="SDA06_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4229754.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SDA06_002507"
     CDS             2453..3316
                     /locus_tag="SDA06_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4229755.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="SDA06_002508"
     CDS             3421..>3875
                     /locus_tag="SDA06_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4229756.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DARDHD010000198         3869 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000198 DARDHD010000000
VERSION     DARDHD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.201"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            187..642
                     /locus_tag="SDA06_002509"
     CDS             187..642
                     /locus_tag="SDA06_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229757.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            761..1819
                     /locus_tag="SDA06_002510"
     CDS             761..1819
                     /locus_tag="SDA06_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG4229758.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1861..3810
                     /locus_tag="SDA06_002511"
     CDS             1861..3810
                     /locus_tag="SDA06_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4229759.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
BASE COUNT         1431 a          563 c          776 g         1099 t
ORIGIN      
        1 tattttattg ctaattagtc ttttatgatg actgaaatag ctaaaataaa tatcttgttg
       61 aataattgct gatactttaa gggatatcta aatttgctga ttgatttatt tatgttatta
      121 tgatattata ataacataaa tgtgttacgc ccgaacctca ttttaaaaaa atgaaggggt
      181 gagacaatga atgctattaa ttggcatgaa tttgttaact atatggagag cttactagat
      241 gatctaaaaa gttttagtat tgctgatgca gtaaagcatg ggttaccagt tagtacaacg
      301 atgaaattac gaggattgaa aattccagat gaaattatta agctgagaac taaaacaatt
      361 attgaaaccg caaaagtcgt tgacaatatg aaccatgaac taaaaggcaa gaaaacaact
      421 tacgagaaag attttgaacg ttttttaaaa tctgttattg gaaaatatct aggtaaaaaa
      481 tctttcagtg aattgagaga tgaagagatt gtacctactc caatggttag caaaatccga
      541 aaaaacaaag ctgaggggaa ttactatctt ggtacaatag caaaaatcat tgattatttg
      601 gaaaataaag agcaaaagaa aggaacgaag aaaggaagat aatttttgtg ttaaatatta
      661 atggggaaga atataccgaa atgattcaaa atcccgaacc tagaattacc gaaaaaacgt
      721 ctgattgttt gctcttttat gaaagggggc agacaaactg atggaaaaaa acgtcaattc
      781 aacatctacc aaagatcaat acagtattat tggagggttg cttggtgata aggataagag
      841 aacacgtcac ggattacgca ttatgcttgc aacttttcta ggagtgcttt ttttattaac
      901 aggcttgggt agctacattt atcaattagg aaactataat tattatctcg tgaacaatac
      961 aacacctata gggtcttcat tggagtttgc tcgatcagga gcagaaatca ctatttcaga
     1021 tgtgtggaca gatcaaaata gagatgtaac agtagtaaaa cttggttact cccaaaacgc
     1081 tagaaagttg ttatctacgc aaggaaaaaa ctataactta tatatggcaa caaaagaaaa
     1141 agatcgtcct aagaagcttg aaatgagcta tgggatgtta agtactgatg gggatgcttt
     1201 cttattccta aaaggtaaat tagaggaaag agcttatcaa gtttttattg caaaccaagt
     1261 atcactaatt tctggtgatg atacagctga cgatgatacc aaagtcaatg tgaatgaagc
     1321 agattcagtt acaaaagcac tttctcaata ttctatgaat gatgttaata atgaaggagt
     1381 aatttctttt gggaaaaaag caaaacctgt taatactcct gataatatca atttcagaat
     1441 aaatccatat tcagattcaa ctaatgtcta tcaaggaagc tttcttacca aagatggcga
     1501 tattgattat tcaaaagttg tagcagttac aagtaagaat aatgttgtaa ataagctaga
     1561 agatcagtta aatgcttcaa atactaacat caagaaaatt gaagccacaa aagctgagta
     1621 tctggaacgt ttacaacaaa atcctgacga taaagattcg caagaaagta ttcgtcaact
     1681 agacagtcga atggagaccg aaattaaagc aatcaatgat acacaaaaat tgattgatca
     1741 atacaaaaat gctgacttta acaaagattc ttttggagac atgaagacga catttaagta
     1801 cctaactttg actgactaga agtaaattat tcttatttta gaggaagtga acacaaacag
     1861 atggaaaata gtaccgagtt ttttaagaat attcaaaccg aatttaatca tgctctcaca
     1921 ccaatgaaac caccaaaaca taagttagtg ggacgtgatg acgaactcca tgagttgtca
     1981 ctgatcttga atagaccgga aacaccgcaa gctatcttgt taggtgaagc cggaacaggt
     2041 aaaacagcac ttgcaagtga atgggctgaa agacagcgac aagatggaaa gaaagtacaa
     2101 gtatataaat tatcaatcgg acaattgcgt gttgggggag atgatgtcct ccaaacacgt
     2161 ttagaaacat taattccaag attgaaagaa tatgagcgaa ttttaaaagc tgaggacaaa
     2221 gataatgaat ttgtcctctt cattgacgag tttcataaag ttgttagctc ttttgggagt
     2281 ggcaataaaa ttgggggtga ttcgctaaaa gaaccgttag caaatgaatc acttcaaatg
     2341 attaccgcta ctacaagaaa agaatatgaa aactatatca aaagagatga tgccttaggg
     2401 agacggctac aaccgttaga attgaatgaa ctaaaaagcg atgttgtcgt cgatattttg
     2461 agacaatggc taaatacatc ctctgttaaa gatgatcctc aggcttcttt tagattatcg
     2521 caaaggattc ctgatgaatt acttcaagaa atcgtacaag caaacaaact ttatcgtgag
     2581 gatttgtccg aaccggctaa atcaattgct gtaatggagg atttgattgc aaaagatcga
     2641 gtagacggaa atgcttataa tcgagaaagt ctattgcaaa tatttggtaa gtataatatt
     2701 cagctagatt ctgattttga tatggaaaaa cttcaagagg aattaaagtc cataaaagga
     2761 caaccgttag cagtttatac tataacaatg ttgtttaaac gtatgaagct tggatttgat
     2821 cgttttaaag gtcgaaagca accaaaagca actgctctgt ttacaggatc aacaggggtc
     2881 ggtaaaactg caatggctaa agccgtggca agaggacttt atggcgatga aaataaagtt
     2941 ctcttagtta atatgccgga ctacaaaacc aaagatgatg caatgcgttt tagacgactt
     3001 ataggtactc atgtaaggca tgatccttct gcgattattg tgtgtgatga atatgagaaa
     3061 gctggtgtag aaacactcga tgccttttta tcaattttag atgaaggtag gataagtttt
     3121 actgaaagag gatgggatga tcgagaagtt cacgacaccg tttcattgtc aaatacaatt
     3181 ttatttgcga caacaaatgc aggtgcggag atgttcgatt cgttaaaccg atatagctca
     3241 ctatctcgtg ttacaaacaa ggaaaattct aatgaataca gcactgaact aaaaagagaa
     3301 taccgaaaag tcgcatcagg gatcaaacaa gccttgattg gtgaaggtct aaagcctgaa
     3361 gttattaacc gatttggacg aatagttcca ttttgggctt tatccgaaca aactcttctg
     3421 gaaattgctt ataatgaaat gttgaagagt gctgaattag tacgaaaaca tttaaatata
     3481 gatttacgtc ttccacctag gaaagaaaga gtatatggtc aaaattttta tgcagacgat
     3541 ctctccatgc acttagtttt tgacatggct tcacaagagg attctaaatc agggggtgca
     3601 aggaatatct tgcatttagt tgaatctgag tttgaagaag cgattactga agcaataatt
     3661 acttatccaa acgcaaaaat attcgatgtt aagttgagtg aagccggagc gtttgaagga
     3721 aaaggtgatt ctaatgctga aattattgca aagccttccg ttgctgaaag taaagaaaac
     3781 aagattgtat tttcgattgg aggggcgtag ctatgaaatt caagaaatat ttactacaaa
     3841 accttagaat aaaacgatgg gtcgtaaga
//
LOCUS       DARDHD010000199         3862 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000199 DARDHD010000000
VERSION     DARDHD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.202"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            10..900
                     /locus_tag="SDA06_002512"
     CDS             10..900
                     /locus_tag="SDA06_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG4229760.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(973..1797)
                     /gene="nadE"
                     /locus_tag="SDA06_002513"
     CDS             complement(973..1797)
                     /gene="nadE"
                     /locus_tag="SDA06_002513"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG4229761.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1809..3281)
                     /locus_tag="SDA06_002514"
     CDS             complement(1809..3281)
                     /locus_tag="SDA06_002514"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG4229762.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1128 a          793 c          682 g         1259 t
ORIGIN      
        1 gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct
       61 agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat
      121 ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag
      181 gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc
      241 gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca
      301 tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg
      361 atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg
      421 gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta
      481 aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct
      541 ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga
      601 acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat
      661 ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg
      721 aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac
      781 acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta
      841 tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga
      901 tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt
      961 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
     1021 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
     1081 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
     1141 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
     1201 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
     1261 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
     1321 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
     1381 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
     1441 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
     1501 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
     1561 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
     1621 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
     1681 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
     1741 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
     1801 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
     1861 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
     1921 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
     1981 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
     2041 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
     2101 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
     2161 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
     2221 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
     2281 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
     2341 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
     2401 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
     2461 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
     2521 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
     2581 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
     2641 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
     2701 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
     2761 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
     2821 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
     2881 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatc
     2941 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
     3001 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
     3061 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
     3121 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
     3181 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
     3241 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
     3301 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
     3361 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
     3421 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
     3481 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
     3541 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
     3601 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
     3661 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
     3721 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
     3781 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
     3841 caatcagtct cgccttgttt tt
//
LOCUS       DARDHD010000200         3846 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000200 DARDHD010000000
VERSION     DARDHD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.203"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1371
                     /locus_tag="SDA06_002515"
     CDS             <1..1371
                     /locus_tag="SDA06_002515"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4229763.1"
                     /translation="SKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAF
                     VQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDA
                     YEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLN
                     VRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPY
                     TFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMA
                     IVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRD
                     VLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNI
                     PRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALE
                     VFKHEQ"
     gene            1361..2578
                     /locus_tag="SDA06_002516"
     CDS             1361..2578
                     /locus_tag="SDA06_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4229764.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2591..>3846
                     /locus_tag="SDA06_002517"
     CDS             2591..>3846
                     /locus_tag="SDA06_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4229765.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1327 a          661 c          802 g         1056 t
ORIGIN      
        1 tcaaaagaag atctgattgc cacaatcgtt gatattttgg gatatgcaat tgcaccagag
       61 tatcttttca acgtcttagc ggatcaagca aaacaagcaa cgttccaatt gaacgatcta
      121 aacaaagcct ttgtgcaatt ggcatctacc tacaatcaat ttaatgggtt atttgatgat
      181 gtggatctcc aatcaaaaaa actaggaaca gatgagcaac aacgaaatgt gacgattacc
      241 gaagtcatca agaaattgaa tgatgtagat gttttagaac acgatgggga cgtgatcggg
      301 gatgcgtatg agtttctgat cagtcaattt gcttcagaag cagggaagaa agccggagaa
      361 ttttacacac ctcatatggt ttctgatatg atggcgcaaa tcgtaacgct cgatcaaaag
      421 gaacgacgat tctttagtgt ttttgatcca acgatgggtt caggttcatt gatgttaaac
      481 gtacgtaact acttaacaca cccagataac gttaaatatc atgggcaaga actaaataca
      541 acgacataca atctagcaaa aatgaacttg attttacatg gtgtcgacgc cgaagaaatg
      601 aatctccgca atggggatac gttgaataag gactggccaa cagatgaacc ttacacattt
      661 gatgcagtag taatgaaccc accttattcg gctaattggt cagcagatac gactttctta
      721 gatgattctc ggtttaatcg ttatgggaaa ttagcgccaa aatccaaagc agattttgcg
      781 tttcttcttc atgggttcta tcatttgaag gaaacaggta ccatggccat cgtcttacca
      841 cacggagttt tattccgtgg cgctgcagaa ggggtcatcc gtcaaaaatt actagaagat
      901 ggcagtattt atgctgtgat cggtatgcct gcgaacttgt tctttgggac atcgattcca
      961 acgacggtga ttgtgttgaa gaagaatcgt caaactcgag atgtattatt cattgatgct
     1021 agtcgtgaat ttgttaaagg gaaaaatcaa aataaactat cggaagaaaa cattcaaaaa
     1081 atcctcgaaa cctacgctga aagaaaagat gtcgagaaat acgctcattt ggcaaccttt
     1141 gatgaaatca aagaaaatga ctataactta aatatcccac gctatgtaga tactttcgaa
     1201 gaagaagaac caattgatat ggttcatgtc ggaaatgaca tcaagaagat acgacaagaa
     1261 cagcaagtgc tagaaaaaaa acttttagaa gctatctctt ccctgcaaac aacacctgaa
     1321 aatgaagcgt ggttacaagg tgcgctggag gtgttcaaac atgagcaatg atacacaacc
     1381 agaaatccgt ttcccaggat ttacagatga ttgggaacag aggaagttgg gggacttcgt
     1441 tgtagattat gttgaaaaga caagtgtaca aaatcaattt cctatgctaa cttcatctca
     1501 acaaaaaggg atagttttgc aagaagacta ttttgcaaat cggcaagtaa ctacagaaaa
     1561 taatattggt tactttgtac tacctagagg atatttcacg ttccgtagtc gaagtgataa
     1621 tgatgtgttt gtgtttaacc gtaatgacat tattgataga gggattataa gctattttta
     1681 tcctgtattt actttaaaat ctgctgattc cgacttcttc ttaagacgta ttaacaatgg
     1741 aatccaacga caattatcta ttcaagcaga gggaacaggt caacatgttc tgtctctaaa
     1801 aaagtttaaa aatatagtag caatgtttcc tagtgaggaa gaacaacaaa aaatcggcac
     1861 cttcttcaaa caacttgacg acacgatcgc tcttcatcag cgtaagttag atttgctgaa
     1921 ggaaacgaaa aaaggattct tacaaaaaat gttccctgaa aacggagcaa aagtgccaga
     1981 aatccggttt cctggtttta cggaagattg ggaagagcgt aagttgggag aattaggtaa
     2041 aactcaatcg ggaattggat ttcctgatgc cgagcaaggt ggttctgaag gtactccatt
     2101 ttttaaagtt tccgacatga ataacattgg taatgagtat gaaatgagaa atgcaaatca
     2161 ttatgtatct aatgagcaaa ttgagcgtaa aaaatggaag ccaataaaag atgtgccagc
     2221 gattatattt gctaaagttg gcgcagcaat aatgcttaat agaaaacgtt tggtcacaag
     2281 ccctttctta atagataaca ataccatggc ttatttattt aataatactt gggatatcta
     2341 ttttggtaaa atattgtttg aaactataaa tttgccaaga tactcccaag taggggcatt
     2401 gccttcttat aacagttctg atattgagaa tatcagtgtt aaggttcctg taaaggatga
     2461 acaacaaaaa atcggtactt tcttcaaaca gctagatgat acgatcgctc ttcatcaacg
     2521 taagttagat ttactaaaag aaacgaaaaa aagcttctta caaaagatgt ttgtctagga
     2581 gggaagaaga atgaaaatta gtcaccgtga ggaagcagaa gtcgaagaac agttgattcg
     2641 tgtacttgga gaagggcata atcagtggac gtatcgtccg gatctaaaat cagaagaaga
     2701 cctttgggtc aatttacgcc aaaaaattat ctcaaataat caagcagaac tgaatgattt
     2761 tcctttgacc gataaagaat ttgaaacgat caaaacggaa ttactgctgc gaacgaaaac
     2821 cccatttgat gctgcgaaat ggttaaaagg tgaaaatggg atggctcgga tcacgattga
     2881 acgggaagat tcgcaattgg gttctgtctc acttatcctt tattccaatc aagacattgg
     2941 tggaggaatc tctacttatg aagtggtcca tcaaattgca aaacgtggaa gtaacataga
     3001 agcacgggat cgacgttttg acgtcacctt attaatcaat ggattaccga ttgtccaaat
     3061 cgagctgaaa caagtaactg caaaagatgg tgtttatcag gcatttaacc aaatcaaaaa
     3121 atatgcagaa gaaggtatgt ttcggaacaa tatcttttcg accttacagt tgtttgtggt
     3181 ttccaatgaa caaaccactc ggtattttgc aaatgcctta ccgaaagact tgcaccctaa
     3241 gtttttattt agttggcgaa cgaaagacaa tgaaaaagta gaaaacttat atgagttttg
     3301 taaacaagtg ttaaatatcc cggatgctca tcgtttgatt gcggattata caatcgtcag
     3361 tgaagatcaa gacaataaaa ccctcatggt gttacatcct tatcaagtac atgcgattca
     3421 agccttgttt attgccgcaa ataaacatca atcagggtat gtgtggcatg cgactggttc
     3481 aggaaagacg ttgactagtt ttgtatctac taagctttta gcacgaaaat cggggatcga
     3541 tcgaacgatc atgttagtgg accgcaaaga tttagataat caaaccacca cagagtttac
     3601 aaaatttgct tcggaattta atactgggat ttcttccggg aacgccaaag cgaatagctt
     3661 gatcgttgga acgggaagtg ccaaagaatt aagtgaaacc ctgttggcag atgccaatgc
     3721 aaatgttgta atcattacaa cccgtcaaaa actagatgcg gcgttgaaat atgcaaagaa
     3781 acaagaagag aaaaaaggaa ccaatcgctt ccaaaaatta atgggccaac atatcgtgtt
     3841 tgtagt
//
LOCUS       DARDHD010000201         3841 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000201 DARDHD010000000
VERSION     DARDHD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.204"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..887)
                     /locus_tag="SDA06_002518"
     CDS             complement(<1..887)
                     /locus_tag="SDA06_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HEG4229766.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTD"
     gene            complement(891..1301)
                     /locus_tag="SDA06_002519"
     CDS             complement(891..1301)
                     /locus_tag="SDA06_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4229767.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1319..1810)
                     /locus_tag="SDA06_002520"
     CDS             complement(1319..1810)
                     /locus_tag="SDA06_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4229768.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1820..2611)
                     /locus_tag="SDA06_002521"
     CDS             complement(1820..2611)
                     /locus_tag="SDA06_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4229769.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2604..3377)
                     /locus_tag="SDA06_002522"
     CDS             complement(2604..3377)
                     /locus_tag="SDA06_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229770.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3533..>3841)
                     /locus_tag="SDA06_002523"
     CDS             complement(3533..>3841)
                     /locus_tag="SDA06_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229771.1"
                     /translation="FLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIER
                     IMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF
                     "
BASE COUNT         1201 a          799 c          518 g         1323 t
ORIGIN      
        1 ttgtctgtct tttgaataat ttgatgtgga acaagtgcac cgccccaatc ccagttattt
       61 tgatcgttct ccccttcttt tgttgccacc cagccagtta gaaaacggcc agtttcatta
      121 gttccgactg ttcttgctgc ataataggct tttccgtcga aagcttcgtc tactggtgat
      181 ttccaatctg aaaataatcc tgctccgact cgatatttag tctttttatc ttcacaatac
      241 tctgaaaaaa gtaaatacca cgttccatca atttcaaata tatcaggcat ttcaatcatg
      301 ttaaattcat tattagccca gtagtctcct ttgaaattcc aatcttttaa atcttcggat
      361 tcaaaataga ccaatctccc cgtcggcttt ttcttacttg ctggttttct agctcctagt
      421 atcaaaacgt attttccatg atcttgatca aaaattgcaa aaggatcgcg ccaatcattc
      481 ttatcatatc cttcttgagg aacaagtttt gctgcttcgc catccttttt ccaatgaatt
      541 aaatctttag aacttgcaat cattaacact tctgaagttt ttccatccaa cttagctttg
      601 cgattatgtc cagtatacaa agctaaatac tcattgattc cttggacaat acttccagcg
      661 taaatgtatt gatcaatgtc atcgttgcca ccacgatgaa ttacttcccc gtattcttca
      721 aatttcacaa aatcagtggt tgtaactagt gaccacccaa aaggatcagt taaaggttta
      781 ggatttctcg tatctcgttg atgaaataga taaaaagttc catctttata aaaaggcatg
      841 caatccccaa accaaaaatc tttaggcttg taaaaaattt tttgcattta tcactcatcc
      901 tcttcgttag tatgtctttg catatctgca agacacgcaa ttgtattcat atttcttaga
      961 ttttcgatga tatcatcgct tttttttcct tgtaatctca aattgacagc ctcaatcaaa
     1021 attcctaaat tcatgccacc aagaagttga aacccatttt gaaatacttc acttctttta
     1081 gcaaattcgt tgaaaggtgt tccaccaatc aagtcagcaa ataccataac ttcttgattt
     1141 tttacttgag aaactatttt ttcaatgttt tcacggaatt cttcaatgcc tgcatctcca
     1201 agtgttacca catgacaatt taaattatcg ccaaaaaaca tttttaatgt atctacaaat
     1261 ccaatggcaa tatttccatg agatgtaatt ataaactcca tccactcatc ctctgttctt
     1321 aataaataaa atcctttaaa aattcttttg aatctctagg agtttgtatc actgtcactt
     1381 caactccatc actaattagt tctttaaaag cctctctctc ttcttcagta atatttatcg
     1441 tcggacggat gcgttctgtt ccattccttc ttgataaatt tcctacgttt acacttgtta
     1501 ttggtaaacc gttatcaatc aaaaatttaa catcgattgg agaaactgca acaataagaa
     1561 ctttttgtcc ttcatattta ccagaaagaa tatttttaac tgctacttcc cttgtaatta
     1621 ttgaagttct aacattaggt ggtgcagcga ggcgaactac agcttttttt acgtcatcat
     1681 tggctacttc attattaata accataattc tgttagcact caattgattt gtccattgtg
     1741 ttgctacttg tccatgaatc attcgatcat caatacgaac atgtataata cctttcattt
     1801 tttcatccat attcaacacc tacttcaaaa tatttaacgc tcttaacgca atagaaacta
     1861 ctaagattaa tagaattaca taaactggtt taacctttcg tcctaacaaa taatacacaa
     1921 ttgctactac acatgcaggt aataaaccag gcataacttg atcaaagatt tcatttccgg
     1981 tcattgttaa gtcgccttgt ttaaattcaa agccaacatt cacttttaca actgtggcaa
     2041 tgagtgcacc gaccaccatt aaccctaaaa ttgatactgc atcagtaata gactttaatt
     2101 ttgtttcaat ttgagttagt aggctcgcac ccgattcttt cccagcaagg tataaaggat
     2161 aagcagtaat ccttaataaa attgatgcca gtatccacaa aataattcca acaaaactac
     2221 cttccaatgc catattagct gcaattgctc caaaaatagt ccatggaata actacaaata
     2281 cactatctcc aattcccgct aaaggaccca ttagacctgt tttcagagca cttactgctt
     2341 ccaaagactt atttttttct tctttttcaa ttccaacatt cataccaata atgtaaggcg
     2401 ctgtatacgg cgtagtgtta aaaaattgta agtgagctaa aacggattgt ttcattcctt
     2461 caggatcatc tttatatgtg tccttgataa aaggaagcat agaatacgca taacccaacc
     2521 cttccatttt ttcataattt aatgaagttg caccattgag aatccaccgg aaaattactt
     2581 gtttgtcgat ttttgtcagt ttcttattca tcttcgtcaa aacctcctgt tgcagctggt
     2641 gctacttgag tattatttga tctattttta tatacgatta gtgcgattgc agcgccaagt
     2701 aacgaaatcc ctaaaattgg tatttggaaa taaactgcca aaacgaaacc aactaacaaa
     2761 taggcaaagt aatcttttgt aggtaaatat ttaagtagca ttcctatccc aacagcaggt
     2821 aatagtcccc ctgctacctt taaaccaccg gtaacccatt caggaatata attgattgca
     2881 cttcccacta acgttgggcc taatatcaca gttaacaaaa caggaattcc agatacaaag
     2941 cttgtattaa ttgatgccaa atattgcatc cactctaatt tttgataatc ttctttttgt
     3001 gcgaattttt ctgccttttg ttgaaaaaaa atgtttgttg accactttag aacatctagt
     3061 tgtacaatta acattgctac cggtattgct aaagttattc ctatagaagc atcctggtta
     3121 gttgagattg ctaaataagt accgatcaat gctgctgttt gataatccgg tactgatgca
     3181 ccaccaaaac tagtaatacc tagtgtcatt aattgcaaag tcccaccgat ataaagtcca
     3241 gtttgaatat ctcccataat aagtccggtc aagaaacctg caacgacagg ttgggaggtt
     3301 ccgattttgg gcccttcctt atcccagtta atgaaaaatc catacaacac aactaaaaat
     3361 gtttgccaaa acgccatttt gttctctcct ttcttcttat acttttatat aagcaattac
     3421 tgtgccaact aacattatcc aataaagcgg gaaaatacag taaatattaa ttaaatgagc
     3481 gtctaaatat actttggaca ccctttcatt tttttagacg ctagtatgct atttagaaaa
     3541 tagtctgctc aaaaattgta tagattaact catactctcc tttgggtatt tcaatacagt
     3601 aaatatcttt gatttctgaa aaaaaatctt ttgatacatt taaaaaagta tcaaatagtt
     3661 gcgctccctc tttcaaatgt tcatttgtta ttccatcacc aatcataatt cgttcaatca
     3721 ttccagaaag atgtagaaat agattaattc tcagaaaact tgggattttg acatcgtaat
     3781 atttttcata caaatatagt actttttcta tctcattgaa tacttttttg gaatctaaaa
     3841 a
//
LOCUS       DARDHD010000202         3768 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000202 DARDHD010000000
VERSION     DARDHD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.205"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            126..242
                     /locus_tag="SDA06_002524"
     CDS             126..242
                     /locus_tag="SDA06_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG4229772.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            258..1061
                     /locus_tag="SDA06_002525"
     CDS             258..1061
                     /locus_tag="SDA06_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4229773.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            1296..1532
                     /locus_tag="SDA06_002526"
     CDS             1296..1532
                     /locus_tag="SDA06_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229774.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1539..3491
                     /locus_tag="SDA06_002527"
     CDS             1539..3491
                     /locus_tag="SDA06_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG4229775.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3563..>3768)
                     /locus_tag="SDA06_002528"
     CDS             complement(3563..>3768)
                     /locus_tag="SDA06_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4229776.1"
                     /translation="EQIYNLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQK
                     GIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT         1350 a          579 c          719 g         1120 t
ORIGIN      
        1 gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag cgggaacaaa
       61 taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt
      121 atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct atggttatgc
      181 ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt
      241 gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag
      301 gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa
      361 gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta
      421 caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca
      481 cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg
      541 caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg
      601 cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa
      661 ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca
      721 aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat
      781 gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat
      841 tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc
      901 ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta
      961 ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca
     1021 aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag ttttggagta
     1081 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
     1141 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
     1201 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
     1261 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
     1321 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
     1381 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
     1441 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
     1501 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
     1561 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
     1621 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
     1681 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
     1741 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
     1801 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
     1861 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
     1921 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
     1981 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
     2041 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
     2101 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
     2161 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
     2221 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
     2281 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
     2341 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
     2401 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
     2461 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
     2521 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
     2581 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
     2641 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
     2701 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
     2761 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
     2821 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
     2881 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
     2941 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
     3001 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
     3061 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
     3121 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
     3181 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
     3241 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
     3301 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
     3361 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
     3421 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
     3481 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
     3541 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
     3601 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
     3661 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
     3721 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctc
//
LOCUS       DARDHD010000203         3743 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000203 DARDHD010000000
VERSION     DARDHD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.206"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..316)
                     /locus_tag="SDA06_002529"
     CDS             complement(152..316)
                     /locus_tag="SDA06_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229777.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="SDA06_002530"
     CDS             611..1939
                     /locus_tag="SDA06_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG4229778.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="SDA06_002531"
     CDS             2141..2974
                     /locus_tag="SDA06_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG4229779.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="SDA06_002532"
     CDS             2990..3727
                     /locus_tag="SDA06_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG4229780.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1251 a          589 c          778 g         1125 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg aca
//
LOCUS       DARDHD010000204         3731 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000204 DARDHD010000000
VERSION     DARDHD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.207"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(579..1136)
                     /locus_tag="SDA06_002533"
     CDS             complement(579..1136)
                     /locus_tag="SDA06_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229781.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1315..1926)
                     /locus_tag="SDA06_002534"
     CDS             complement(1315..1926)
                     /locus_tag="SDA06_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229782.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2039..2296)
                     /locus_tag="SDA06_002535"
     CDS             complement(2039..2296)
                     /locus_tag="SDA06_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229783.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2893..3171)
                     /locus_tag="SDA06_002536"
     CDS             complement(2893..3171)
                     /locus_tag="SDA06_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229784.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3205..3456)
                     /locus_tag="SDA06_002537"
     CDS             complement(3205..3456)
                     /locus_tag="SDA06_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229785.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1121 a          768 c          503 g         1339 t
ORIGIN      
        1 gcctccttat tccgaataaa tggtgtccaa aagcttgcat ctgccagatt atctcatttc
       61 ttatgacaag ctaagcaaat tatctccacc aaataattta ttcatatttt tgacttgcgc
      121 atactaacta attcttaaat tttcatacaa aatttctcaa tttcccttaa cgagtaatat
      181 ttatgtacgg tgattctcac tagtatccca atcacttttt aagttatccc gatttcaatt
      241 ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt catttgttga
      301 gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc gtctaccaaa
      361 cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat tcgtataaag
      421 ctaacaacta ttaactcctc tcagctaata gttgctctct tttttattca gattcatttt
      481 tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg ttccattaca
      541 acattcactg gctgatgaaa tggattagta gatttcgatt attcaaagcg attttctatt
      601 ggtggatcgt gttcccaact aataagacgg attccaaacc tatcgtacag atcttctaaa
      661 ccacttataa attttgcacc tatttcaggt gttttattgc gtggatattc tattgttacg
      721 ccattaaatg gataccaatt aggattaggc tctctttttt tctgtacatc tcccatagaa
      781 tagttagcac tttctgggac ataatatatt cttcgaccat cctctaattc atctaataag
      841 atatttcgtg atcgtctatc acttgctaaa tgtagacgcc acgtgcgata ccatgccatg
      901 ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc
      961 ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt aatttctggt
     1021 cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac
     1081 acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt
     1141 cgcacatcac ctctacattc tcacatatta gttcgtaata ctcctttaat atcaaaatat
     1201 ctactggtcg taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc
     1261 atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc
     1321 accaaaattg ttaacaatcg gttcagatat ttcccagttg attaaggtaa ttccgtattg
     1381 tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt cttatgtatc cactttctcc
     1441 taatataaag tctacctctc ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg
     1501 ttttaatgac attgattgta cattttcccc catataatat tgcgcacttc ctcgactaaa
     1561 atagattcct agatcgtccc caaaaggtag atcttctcta ccactagcta atctacttct
     1621 ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa actcagctat
     1681 gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc taaatggaaa
     1741 ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa agaaaggaaa
     1801 tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg atgtaatcca
     1861 aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc ccctattact
     1921 attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt ccataccttg
     1981 attttttact gagttaccta tgcgagatat cattctccac taaggtgcat catattgtct
     2041 atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca tttaatgcat
     2101 ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct aattcataag
     2161 cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata gctactgaat
     2221 ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata gtaggattta
     2281 cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt ttttgaacta
     2341 cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca ctaaggaata
     2401 taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt aaatcattat
     2461 actgtatctc atacattatt atcaataaac tattataaaa atgcaaaaaa tgctttatca
     2521 atctattttg taattggtca taatatcatt gaaatcatac cctgtatcga tttttcctat
     2581 gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac agacactgga atggtaattt
     2641 tacccactat tttgctccaa ctatttgtaa tcgtacccac ttttgaaata ccgagatgat
     2701 cattttaatt gatatgctga ttcccaagca aaaccccaag aaattaaatg ctctatcgca
     2761 tctagcttct tgggttttgc ttatataaat tccaacattt aaatttttta tgctgttcgg
     2821 tctcagaaat gccgtttttg cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc
     2881 cgatgacttt gattaatttt tttcaaattg tttattaaga ctaatgctgt ctttttgcag
     2941 ataccaatgt tcgaactttt taacattatc atatgatcct gcaagtttta acttcttacc
     3001 attagatgtt atcaaagtta taattaacct tgcctttttc ccattaggat cttttatttt
     3061 atgaattttc tgcaggtaat ctctcctaat ggcagctatt tttacctcat cagtgctgat
     3121 aaaccattga tcatccacat acatactgcc tgttatgttg tgtttttcca tttcttccga
     3181 gaacgaaaag tttaaggctc gttcttactt ccaaagttga atgagtccat ggattggact
     3241 tagtgtgaga attgagaaca acaataagaa tccatatcct cccgcaaaca tccctaaggc
     3301 tatactgtct ctccatccat aagtaggatt agaagagaaa agagtcaccg tttgtaataa
     3361 tatgaaaagg aataatccag cacaaacacc aaagataaca actccaaaca ttgaattttt
     3421 atacaatgac catttattta taggcttttc tttcatttta cttcccccta ctgttctgtt
     3481 cacacggaat gaattcaaag ttcataagcc ctcctctttg aacatatgaa tacgacttgc
     3541 taactatact ttaaaaaagc ttgttttaag cggttatagg cgagttgtaa tctctattca
     3601 tctcattagc aaataacttg ccgaaaagtc tactgaaatt aaagcagata gtggaagaat
     3661 tatttagcca ggatagacac tatttgtgaa acacatgtct ttacttttga aattctatga
     3721 tgaaaagcct t
//
LOCUS       DARDHD010000205         3698 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000205 DARDHD010000000
VERSION     DARDHD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.208"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            75..470
                     /locus_tag="SDA06_002538"
                     /pseudo
     CDS             75..470
                     /locus_tag="SDA06_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="SDA06_002539"
     CDS             complement(465..683)
                     /locus_tag="SDA06_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229786.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="SDA06_002540"
     CDS             790..1104
                     /locus_tag="SDA06_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229787.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="SDA06_002541"
     CDS             1208..2386
                     /locus_tag="SDA06_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4229788.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="SDA06_002542"
     CDS             2789..3082
                     /locus_tag="SDA06_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG4229789.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3283)
                     /locus_tag="SDA06_002543"
     CDS             complement(3170..3283)
                     /locus_tag="SDA06_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEG4229790.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         1263 a          546 c          716 g         1173 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaa
//
LOCUS       DARDHD010000206         3673 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000206 DARDHD010000000
VERSION     DARDHD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.209"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SDA06_002544"
     CDS             127..579
                     /locus_tag="SDA06_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229791.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SDA06_002545"
     CDS             593..1282
                     /locus_tag="SDA06_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229792.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3673
                     /locus_tag="SDA06_002546"
     CDS             1407..>3673
                     /locus_tag="SDA06_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG4229793.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYL"
BASE COUNT         1240 a          487 c          759 g         1187 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tga
//
LOCUS       DARDHD010000207         3533 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000207 DARDHD010000000
VERSION     DARDHD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.210"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(495..1058)
                     /locus_tag="SDA06_002547"
     CDS             complement(495..1058)
                     /locus_tag="SDA06_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4229794.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1073..2116)
                     /locus_tag="SDA06_002548"
     CDS             complement(1073..2116)
                     /locus_tag="SDA06_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229795.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2249..2653)
                     /locus_tag="SDA06_002549"
     CDS             complement(2249..2653)
                     /locus_tag="SDA06_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4229796.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2886..3521)
                     /locus_tag="SDA06_002550"
     CDS             complement(2886..3521)
                     /locus_tag="SDA06_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4229797.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         1092 a          726 c          588 g         1127 t
ORIGIN      
        1 aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta
       61 atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct
      121 taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca
      181 tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt
      241 actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat gagtgaatga
      301 tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga tggtcattca
      361 tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac tagaacaaat
      421 caattaaatt ccataaaata taaataatag acctagcatc atctggtata aaatggatgc
      481 taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat catttctatt
      541 ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag tttagataaa
      601 tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa taacttccaa
      661 tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc aactaaccta
      721 attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac caagaaactt
      781 tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa tcttaaatca
      841 actagtttag aattactaat atcacagtaa ggcacactgg accaatttac acgtccctct
      901 tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga atctgtatag
      961 ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac atcgctagat
     1021 gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc gactattttt
     1081 tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct aagtctagta
     1141 ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg ccactttcaa
     1201 acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt ggaaatttga
     1261 ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc gaggtataag
     1321 gaccataaga acgactggct aagagataat ggccataaaa agccacacct tgaatcttat
     1381 cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg cgaattgctg
     1441 ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca ccttttagtg
     1501 tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct tgtggaatct
     1561 ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca gatgtatctg
     1621 ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct gtgattgtca
     1681 accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc cgtttaggta
     1741 aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata atagaatgat
     1801 gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa gccaaccctt
     1861 gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc attcttgttt
     1921 ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct gcttgaacaa
     1981 taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc ttccaatgct
     2041 taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa atcattattg
     2101 ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc catataccgc
     2161 tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga tctttcataa
     2221 tctcatttat agtaacatta agaatccctt attctactat agtcacttcc acatcaatat
     2281 tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg attttctctg
     2341 cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt tggtagcccc
     2401 catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac tcaagattat
     2461 tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca gccgcaaaaa
     2521 gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc atgtgcatac
     2581 ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt gctgtttcgt
     2641 acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg taacgagtat
     2701 tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca acatcgatga
     2761 tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata tttggtgtcc
     2821 ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc ttcacactgc
     2881 tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata gttaaagaaa
     2941 aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact aatcgacgga
     3001 atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa aacaagataa
     3061 aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa caatgggaaa
     3121 agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt cgatagatta
     3181 caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc acctatccca
     3241 tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc aacaaatagg
     3301 tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga ccagaccaag
     3361 aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt agtcatttga
     3421 ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact catcacacct
     3481 aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc cta
//
LOCUS       DARDHD010000208         3520 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000208 DARDHD010000000
VERSION     DARDHD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.211"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..2166
                     /locus_tag="SDA06_002551"
     CDS             106..2166
                     /locus_tag="SDA06_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG4229798.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2249..2818
                     /locus_tag="SDA06_002552"
     CDS             2249..2818
                     /locus_tag="SDA06_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229799.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2993..>3520)
                     /locus_tag="SDA06_002553"
     CDS             complement(2993..>3520)
                     /locus_tag="SDA06_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229800.1"
                     /translation="FYGYFSMWDTFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLL
                     LAVVSFCFSMTVGVMWEFVEFSADKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDI
                     TRTIIESQNADGKTDKTIIQNGYLDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQ
                     KFTFIKNFIPKKIKR"
BASE COUNT         1246 a          640 c          657 g          977 t
ORIGIN      
        1 actctcctaa tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa
       61 attactactt catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga
      121 cgttcaaaac aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa
      181 aaacactacg ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat
      241 atgcatgtac ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct
      301 gagaccactg attcccctat cactattact catactttca ctgatgcaaa tggaaaacag
      361 ctacttcctg cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac
      421 tggttaattc ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct
      481 atcttttccg atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt
      541 ttgaatcatg ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat
      601 gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt
      661 tcgatatctg atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc
      721 aaagtaattt caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt
      781 tccttgtcct attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat
      841 gttcgtgctg atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt
      901 tacgccccta aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa
      961 gaagaaatca aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta
     1021 ttaacttata tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta
     1081 gaagcatctg ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa
     1141 gaacttcaag cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct
     1201 gctgaatttt ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa
     1261 gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg
     1321 gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa
     1381 attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt
     1441 gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta
     1501 ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat
     1561 ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat
     1621 tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata
     1681 gatcaaaaag aacaaaaaag acgagaagaa agcttgtcag aaaaaaaaca acaaatggct
     1741 catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata
     1801 tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa
     1861 gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa
     1921 tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa
     1981 aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct
     2041 agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg
     2101 cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg
     2161 ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg
     2221 cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat
     2281 ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga
     2341 atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg
     2401 ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc
     2461 tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac
     2521 ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc
     2581 agtgaaacca gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca
     2641 agaatttcct accctccata cggacggacc aatctatctt cacacacaga catggagcca
     2701 tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg
     2761 cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg
     2821 aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct
     2881 tacttttttg agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag
     2941 gggcagtgac aaaagtattg tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt
     3001 tgattttttt aggaataaaa ttcttgatga acgtaaattt ttggtaatcg tttcgactat
     3061 agaggtaacc aaatacactg aaaacaactg ctccaataaa attaacaaat aaatccttca
     3121 tagtatccat gattcccacg tctaagtaac cattttggat gattgtttta tctgttttac
     3181 catctgcatt ttgactttca atgattgtcc gagtgatatc tggaacatga tagacagtgt
     3241 ttccgtcttt acttaactcc acactattga tttgctggat gatcgtatct ttttgcatat
     3301 cgaattgcag taatttatct gctgaaaatt ccacaaactc ccacataaca ccaactgtca
     3361 tcgaaaaaca gaaggaaaca actgctagta ataaaggagt caagttcatg gtttttgtat
     3421 ttttattgag caaataaaca agggaaaagc cgacaccccc agctaaaaat ccgctgaatg
     3481 catgtagaaa agtatcccac attgaaaaat agccataaaa
//
LOCUS       DARDHD010000209         3461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000209 DARDHD010000000
VERSION     DARDHD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.212"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..146)
                     /locus_tag="SDA06_002554"
     CDS             complement(<1..146)
                     /locus_tag="SDA06_002554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG4229801.1"
                     /translation="MSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIVE
                     ATYS"
     gene            complement(139..1338)
                     /locus_tag="SDA06_002555"
     CDS             complement(139..1338)
                     /locus_tag="SDA06_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4229802.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="SDA06_002556"
     CDS             complement(1468..2844)
                     /locus_tag="SDA06_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4229803.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3461)
                     /locus_tag="SDA06_002557"
     CDS             complement(2844..>3461)
                     /locus_tag="SDA06_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229804.1"
                     /translation="ANSKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSLLAGLDKP
                     QTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSADESILFE
                     LGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILK
                     TLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1059 a          652 c          482 g         1268 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacctacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg c
//
LOCUS       DARDHD010000210         3361 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000210 DARDHD010000000
VERSION     DARDHD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.213"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SDA06_002558"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SDA06_002558"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG4229805.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="SDA06_002559"
     CDS             complement(2261..2512)
                     /locus_tag="SDA06_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG4229806.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="SDA06_002560"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="SDA06_002560"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG4229807.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          917 a          738 c          626 g         1080 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 t
//
LOCUS       DARDHD010000211         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000211 DARDHD010000000
VERSION     DARDHD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.214"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SDA06_002561"
     CDS             301..585
                     /locus_tag="SDA06_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229808.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SDA06_002562"
     CDS             588..1004
                     /locus_tag="SDA06_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229809.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SDA06_002563"
     CDS             complement(1141..1860)
                     /locus_tag="SDA06_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229810.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SDA06_002564"
     CDS             2535..2630
                     /locus_tag="SDA06_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4229811.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SDA06_002565"
     CDS             complement(2932..3177)
                     /locus_tag="SDA06_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229812.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARDHD010000212         3129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000212 DARDHD010000000
VERSION     DARDHD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.215"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SDA06_002566"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SDA06_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SDA06_002567"
     CDS             1360..1992
                     /locus_tag="SDA06_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4229813.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SDA06_002568"
     CDS             2197..2424
                     /locus_tag="SDA06_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG4229814.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SDA06_002569"
     CDS             2493..2759
                     /locus_tag="SDA06_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4229815.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SDA06_002570"
     CDS             2749..3018
                     /locus_tag="SDA06_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4229816.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DARDHD010000213         3104 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000213 DARDHD010000000
VERSION     DARDHD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.216"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(200..1252)
                     /locus_tag="SDA06_002571"
     CDS             complement(200..1252)
                     /locus_tag="SDA06_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229817.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1265..2203)
                     /locus_tag="SDA06_002572"
     CDS             complement(1265..2203)
                     /locus_tag="SDA06_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4229818.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2654..2968)
                     /locus_tag="SDA06_002573"
     CDS             complement(2654..2968)
                     /locus_tag="SDA06_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229819.1"
                     /translation="MYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENII
                     LRTEYGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSH
                     RQ"
BASE COUNT         1012 a          650 c          449 g          993 t
ORIGIN      
        1 catattttta ttctagttgt cttttgaaca atcaataaca taaaggtgag tttcttctac
       61 attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct cctactcaaa
      121 aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      181 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      241 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      301 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      361 gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      421 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      481 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      541 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      601 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      661 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
      721 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
      781 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
      841 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
      901 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
      961 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1021 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctagtt
     1081 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1141 aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1201 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1261 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1321 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1381 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1441 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1501 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1561 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1621 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1681 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     1741 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     1801 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     1861 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     1921 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     1981 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2041 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2101 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc atcaacccaa
     2161 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2221 tctttctatt atactcattt gagaatcttt tcacaacctc gttttgttat tcttctctta
     2281 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2341 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2401 tagaaaaaat ctcactcctc tgattataaa agagggggca ttaacctcgt gtatactaaa
     2461 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2521 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2581 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2641 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     2701 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     2761 tccaagccca gtctaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     2821 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttata ttgagttgat
     2881 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     2941 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca cctaatgaat
     3001 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3061 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatt
//
LOCUS       DARDHD010000214         3096 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000214 DARDHD010000000
VERSION     DARDHD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.217"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..131)
                     /locus_tag="SDA06_002574"
     CDS             complement(<1..131)
                     /locus_tag="SDA06_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG4229820.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
     gene            1008..1262
                     /locus_tag="SDA06_002575"
                     /pseudo
     CDS             1008..1262
                     /locus_tag="SDA06_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1332..1844
                     /locus_tag="SDA06_002576"
     CDS             1332..1844
                     /locus_tag="SDA06_002576"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4229821.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1841..2878
                     /locus_tag="SDA06_002577"
     CDS             1841..2878
                     /locus_tag="SDA06_002577"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4229822.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1106 a          554 c          601 g          835 t
ORIGIN      
        1 tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg
       61 taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta
      121 aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta aaatagattt
      181 tttggaaaac tttgcaacag aaccgtttta ttgatgacca cgaaagtgca tggcgatatt
      241 tgatgttgat actttatgca gtaccctatg tattcttcgc tatcattctg tggctcatag
      301 cattgttttt tagagtattt aatgaccttt aggtagattt atatataaaa agggctaaat
      361 acgcaaaata cccccttaaa atgcaaaata aatgcaaata agggggtaac tccttatgaa
      421 aaaacaacta atagtattat aacacgtttt tatgccgaga aaacttattg gttggaatgg
      481 gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat taatcccaag
      541 aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag gaaattggaa
      601 taaagcaacg aaaggagttg agtaaaatga gtgagaaaac gaagaaaatc attcgagtat
      661 cattagcttt ttcacaagta gtagtgcaat cattgatagt ttattatact taccagcaat
      721 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
      781 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      841 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      901 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      961 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
     1021 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
     1081 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
     1141 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
     1201 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
     1261 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
     1321 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
     1381 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
     1441 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
     1501 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
     1561 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
     1621 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
     1681 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1741 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1801 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1861 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1921 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1981 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     2041 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     2101 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     2161 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     2221 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     2281 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     2341 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     2401 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     2461 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     2521 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     2581 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     2641 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     2701 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2761 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2821 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2881 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2941 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     3001 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     3061 caataaattc tatcgaagtt tacgcactgc ctctac
//
LOCUS       DARDHD010000215         3054 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000215 DARDHD010000000
VERSION     DARDHD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.218"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..354)
                     /locus_tag="SDA06_002578"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="SDA06_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="SDA06_002579"
     CDS             complement(614..1162)
                     /locus_tag="SDA06_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229823.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="SDA06_002580"
     CDS             complement(1163..2020)
                     /locus_tag="SDA06_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4229824.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="SDA06_002581"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="SDA06_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="SDA06_002582"
     CDS             complement(2582..2911)
                     /locus_tag="SDA06_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4229825.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DARDHD010000216         3013 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000216 DARDHD010000000
VERSION     DARDHD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.219"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..359
                     /locus_tag="SDA06_002583"
     CDS             177..359
                     /locus_tag="SDA06_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG4229826.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="SDA06_002584"
     CDS             462..746
                     /locus_tag="SDA06_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229827.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="SDA06_002585"
     CDS             762..998
                     /locus_tag="SDA06_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229828.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="SDA06_002586"
     CDS             1020..1274
                     /locus_tag="SDA06_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229829.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="SDA06_002587"
     CDS             1356..1541
                     /locus_tag="SDA06_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG4229830.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="SDA06_002588"
     CDS             1608..2003
                     /locus_tag="SDA06_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG4229831.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="SDA06_002589"
     CDS             1993..2370
                     /locus_tag="SDA06_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229832.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="SDA06_002590"
     CDS             2392..2766
                     /locus_tag="SDA06_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229833.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DARDHD010000217         2976 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000217 DARDHD010000000
VERSION     DARDHD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.220"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..997
                     /locus_tag="SDA06_002591"
     CDS             <1..997
                     /locus_tag="SDA06_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4229834.1"
                     /translation="SINIPYGSMASAITSVAEERTALSTFRSIGATLAGMFIGVLTPI
                     FIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKSDESQLKVSLLK
                     SLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIVLMNFINPILML
                     VLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLVLTSVGYMGLGI
                     FNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLSIIGYDS
                     LAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQKHN"
     gene            1008..1802
                     /locus_tag="SDA06_002592"
     CDS             1008..1802
                     /locus_tag="SDA06_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4229835.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            1815..>2976
                     /locus_tag="SDA06_002593"
     CDS             1815..>2976
                     /locus_tag="SDA06_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG4229836.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKE"
BASE COUNT         1036 a          361 c          591 g          988 t
ORIGIN      
        1 atctattaat attccttacg gttcaatggc atcagctatc acttctgttg cagaagaaag
       61 gacagcgtta tcaacatttc gtagtatagg agctacctta gcaggtatgt tcattggcgt
      121 gttaacacca atttttattt atacaagaga tagttctgga gctcaaattg tacgtggtgg
      181 atcaactttt acaattgtgg caggagtatt ttcagtgtgt gcagttattt gttacgtaat
      241 atgttataaa ttaactgtag aacgggttaa aagtgatgaa tcgcagttaa aagtttcatt
      301 gttgaaatct ttaaaaggat tagctaaaaa tagagcgttg ctttctataa tagctgcagc
      361 tatttttttg ttgcttgctc agcttttaac aatgagtatg aacaattatg tatttccaga
      421 ttattataat aatgcacagg gaatagtttt aatgaacttt atcaatccta ttttgatgtt
      481 ggtacttgtt tcacctgtaa gtttaatttt cagtaaaaaa tacggtaaaa aagaattggc
      541 gtcagtaggg atgttcttct ctgctattgt atatttactt ttatttttga taaagcctag
      601 taatatgtat gtattcttag ttcttacttc tgttggatat atggggttag gtatttttaa
      661 tacagtcatt tgggcgaata ttactgatgt aatcgatgat caagaagtga aatctggcca
      721 aagagaagat ggaactattt atgcagtata ttcttttgcc cgaaaaattg gacaagcttt
      781 agctggcggt gtcggaggtt ggactctttc tataataggg tatgattcgt tagcaaaagt
      841 acaaagtgga gaagttttac aaaagctgta taactcttca acgttgattc cagcagtttg
      901 tttctttgtt gtaggattga ttttattttt cgtatatcca ttatctaaag aaaaagtatt
      961 agaaaattat aatttgctaa atcaaaaaca taactaggag aaaaaacatg aacagttgga
     1021 tattttgtat tgattcagat ggttgtgtca tggatacaat gacttataaa cacaatctct
     1081 ttttcgggcc aattgctgca gagatttttg atgttcatca tagggaaaaa ttcctacagg
     1141 attggaataa tattaattta tattcagaga ctcgtggaat aaacagattt ttaggtttag
     1201 ttaaaacatt agaaaaaaat gatgtaccat cgattaatcg attaaagcac tgggtatctg
     1261 taacaaaaga attgtctaat ccatctttga agagggaaat agaatactct cagtcagagg
     1321 atttattaaa agctttaaaa tggtcggaag aagtcaatcg acagattagt caagctaaag
     1381 gattagataa acccttttta agtgctagag atactataaa agctctgaaa aaatatggga
     1441 agataattat tgtaagctca gctaataagg aagctattca agaagaatgg gaaagacatg
     1501 aattattatc cttagttgat gaactgtgtt gtcaagataa aggaaaaaaa gaagacatta
     1561 ttcgaagtgt aattgaaaat ggttgtgatt tggataagat attaatgatt ggtgattctc
     1621 ctggtgatct agaagctgca aataagaata aagttttctt ttatcctatt ttggttaata
     1681 aagaaaaaga atcatgggaa aacttaagaa ctgatatttt atttaaatgt ttatcgggag
     1741 agtatataga tattgaacaa aaatatatag atactttttg gaaaaatctt actaataagt
     1801 agaggagaaa gaaaatgact ataaaagtag acttacgaaa aaaaccatat catttagatg
     1861 aagatgctat ttcttggatt gaagagacga ttgatgctat gactttagaa gaaaaaatag
     1921 gtcaattatt tgttaatatg ggatctagtc gtgataaaaa ttacttaagt agtatggtca
     1981 atcagtacca tattggagct gtacgatata atccaggaac tgctgatgag gtatatgatc
     2041 aaaactatat cctacaaaca gaaagcaaaa ttcctttatt aattgctgct aatacagaag
     2101 caggtggtaa cggagcttgt acagatggta ccgaagttgg tgtagaagta aaaattggtg
     2161 caacaaatga tgcgaaatat gcttatgaat tagggagagt ttctggaatt gaagcatcgg
     2221 ctattggatg taactggagt tttgctccta ttgtagatat taatagaaat tggagaaatc
     2281 ctatcatttc tagtcgatct tttggacaaa gccctgataa agttttagaa cttagtttag
     2341 cttatatgaa aggtattcaa gagagtggaa ttttgcctgc agctaaacat ttccctggtg
     2401 acggtattga cgaacgagat caacatctat cttttagtat taatagttta agtacagaag
     2461 aatgggataa ttcttttgga aaagtatact caggcttaat agaagctgga ttaccttcaa
     2521 ttatggcagg tcatatccat atgccagctt attcaaaaca ttttaatctt gatctgaaag
     2581 atgaagattt acttcctgca actttatcaa aagaattaat cactgactta cttagaggaa
     2641 aactcgggtt taatggatta gtagttactg atgctagtca tatgttagga atgactgggg
     2701 caatgaaacg gtcagaatta ttgcccactt ctattgcggc tggatgtgat ctattcctat
     2761 tttttaatga tccagatgaa gactttaatt atatgatgga aggttattac aaaggaatta
     2821 tttcagaaga gcgtttacgt gatgcattaa gaagaatttt aggcttaaag gctaaactag
     2881 gtttgcataa aaaagaaaag aataaaattt tgttaccgaa acaagaagct atggaaaaaa
     2941 ttggtttaat tgaaaataaa tcaattttta aagagg
//
LOCUS       DARDHD010000218         2937 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000218 DARDHD010000000
VERSION     DARDHD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.221"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(84..626)
                     /locus_tag="SDA06_002594"
     CDS             complement(84..626)
                     /locus_tag="SDA06_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG4229837.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            761..1150
                     /locus_tag="SDA06_002595"
     CDS             761..1150
                     /locus_tag="SDA06_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229838.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1154..1918
                     /locus_tag="SDA06_002596"
     CDS             1154..1918
                     /locus_tag="SDA06_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4229839.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2047..>2937
                     /locus_tag="SDA06_002597"
     CDS             2047..>2937
                     /locus_tag="SDA06_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4229840.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGA"
BASE COUNT          944 a          492 c          603 g          898 t
ORIGIN      
        1 tgttttttaa tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat
       61 tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag
      121 aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct
      181 ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc
      241 cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt
      301 atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg
      361 tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct
      421 aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg
      481 acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc
      541 attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc
      601 atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata
      661 tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa
      721 tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt
      781 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
      841 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
      901 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
      961 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
     1021 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
     1081 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
     1141 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
     1201 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
     1261 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
     1321 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
     1381 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
     1441 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
     1501 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
     1561 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
     1621 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
     1681 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
     1741 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
     1801 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
     1861 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
     1921 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
     1981 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
     2041 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
     2101 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
     2161 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
     2221 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
     2281 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
     2341 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
     2401 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
     2461 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
     2521 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
     2581 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
     2641 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
     2701 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
     2761 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
     2821 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
     2881 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcg
//
LOCUS       DARDHD010000219         2919 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000219 DARDHD010000000
VERSION     DARDHD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.222"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            593..>2919
                     /gene="esp"
                     /locus_tag="SDA06_002598"
     CDS             593..>2919
                     /gene="esp"
                     /locus_tag="SDA06_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG4229841.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1063 a          443 c          569 g          844 t
ORIGIN      
        1 tttaaatatg gacaattcaa ttataaagaa aaagaatcaa taatttaatg aggctgtgac
       61 aaatgtcatt ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact ccaatcattc
      121 gtttaagatt ctaggaatca ttcagaaatg ctgtgaaacc gatactttat aattataaag
      181 aaggtgattt caaattccga agaaggaatt tgaagtcacc tttttactta tgtcacagtc
      241 tcattcagta atgcgggata aggctcctta ttccgttatt tgaatactct gaagtttaat
      301 ctaagaaagg gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag
      361 aaagaataca tacatatctt tttatatgtt ttcagctgct gaatggttaa aaagcacaaa
      421 tatccgaaaa aaacatgaag aaatgtactt tttttgttgt aatgttcatg aaattctcta
      481 agtaaattag ttaacataag gttagtaaga ttcctattct gtgaataata cgtatcagat
      541 ttatctttta ttcttactag gaaagtaatt attaagggag ttgatttgaa taatggttag
      601 caagaataat aagagagtat ttctagaaaa aacaaagaaa cgagtattga agtacagtat
      661 caaaaaatta agtgttgggg ttgcatctgt tttgattggt gttggtcttg tcttgggaac
      721 aactgaacta gttaaagcac aagatgaaat ttctccaagc agcccacttg aaacagcgat
      781 aagctccgta caagtaggtg acaaagtagc ttcggggaat acttttcagg agaatcctgg
      841 ttatacgaaa aattataact tttcggattt acaatttagt cctcaagaac taactggaga
      901 cacactaaaa ggcaatacaa ttggatttga agtttacgga aaacataata ttgcagcatc
      961 aactaaaaac tgggaaatcc gtcttcaatt agatgaacga ttagctaaat atgtagaaaa
     1021 aattcaggtc gatccaaaga aaggaattgg atcttctaga cggacctttg taagaattaa
     1081 tgattcgctg ggtagaccta caaatatttg gaaggtcaat tatattcgtg caagtgatgg
     1141 attatttgct ggggcagaaa caaccgatac gcaaaccgct cctaatgggg taattacgtt
     1201 tgaaaagagc ctagatgaaa tttttaaaga gataggcata gacaatctca agaccgatcg
     1261 tttaatgtat cgaatctact tggtaagtca tcaagacgat gataaaattg taccgggtat
     1321 tgatagtacg ggctactttt taacagattc agatgatttc tataatagct tagatgtatc
     1381 tgaaaataat cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa
     1441 tactcaaaca aaagatggtt caggatccac aggtgctaat ggagctataa tcttagatca
     1501 taagttaacg aaaaattata atttctcata ttcatcttcc gcaaagggca cgccttggta
     1561 tgctaattac aaaattgatg aacgattagt tccatatgta gcgggtatac agatgcacat
     1621 ggttcaggct gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa aagtagctga
     1681 tttagcaatc gagcgacggg aaggtcatga aaattatggc ataggttcaa taactaataa
     1741 tgatttaact aaacttatcg attttgctaa tgcaagtcca cgtccagtcg ttattcgata
     1801 tgttttacaa ttgactaagc cattagatga aatcttagaa gatatgaaag caacagcaca
     1861 agttgaagaa aataaaccat ttggtgaaga tttcatcttt gattcttggt tgtcggatac
     1921 gaataaaaaa ttaatccaga acacttatgg aacaggttat tattatttgc aagatattga
     1981 tggtgatgga aaccctgacg ataaagaaga gagcggagac acgaatccat atatcgggaa
     2041 acctgaatta gaagaagtat atgatgttga cacaacagtt aaggggaaag tattcatcca
     2101 cgagttagcg ggaacaggtc acaaagccca acttgttgat aaagaaggta ctgtattagc
     2161 agaaaaaact atcgctccaa atgaaaaaga tggggctcca atttcagata ctgtagaatt
     2221 tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat
     2281 cgtttctcca ggttttgata aaccagaaga aggttcaacc gttattaagg aatcaccaaa
     2341 agcggttgat aaacaaaccg tggtagttgg atttaaacca gatgctaaag aatcaattcg
     2401 gaataataaa aacttacctg aagatgcaga gtattcatgg aaaacagagc ctgatacttc
     2461 taacgttact gatagtacga aaggtattgt aactgttaag atcggaaatc gaactttcga
     2521 cgtggatgta gagtttgctg taaaagcttc tcaagctatg gaaaatgatg caacatacgt
     2581 acctataaca acaactccag aaacgacagt tcaaagtgga aaacctacat ttgataaacc
     2641 agatgttcct ttagctaaag atgccttttc aatttcagat gtctataata agtacttcgg
     2701 taatgcaagt gttgatgcaa acactggtat tgttacattc actccagcta aaggtgtagg
     2761 agaatcggag ccgattactg gaacaattcc tattaaaatt gtttaccaag atggttctgt
     2821 aggcacgacc gatttagcag taactgtaag taaagatatt tatgaaaatc caggagaaaa
     2881 cattcctgca ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DARDHD010000220         2873 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000220 DARDHD010000000
VERSION     DARDHD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.223"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SDA06_002599"
     CDS             complement(61..1371)
                     /locus_tag="SDA06_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4229842.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SDA06_002600"
     CDS             complement(1383..2801)
                     /locus_tag="SDA06_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4229843.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          898 a          601 c          447 g          927 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cac
//
LOCUS       DARDHD010000221         2855 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000221 DARDHD010000000
VERSION     DARDHD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.224"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            221..304
                     /locus_tag="SDA06_002601"
                     /pseudo
     CDS             221..304
                     /locus_tag="SDA06_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            353..436
                     /locus_tag="SDA06_002602"
     CDS             353..436
                     /locus_tag="SDA06_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4229844.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            561..1298
                     /gene="erm(B)"
                     /locus_tag="SDA06_002603"
     CDS             561..1298
                     /gene="erm(B)"
                     /locus_tag="SDA06_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4229845.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1612..2406)
                     /locus_tag="SDA06_002604"
                     /note="aph(3')-IIIa"
     CDS             complement(1612..2406)
                     /locus_tag="SDA06_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4229846.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2499..2750)
                     /locus_tag="SDA06_002605"
                     /pseudo
     CDS             complement(2499..2750)
                     /locus_tag="SDA06_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          926 a          552 c          479 g          898 t
ORIGIN      
        1 ttttgtacat ttaacttgac ggtgacatct ctctattgtg agttattagt ggtacagttt
       61 tcaaccgttt taattataaa aaagtggtgc atttttaaat tggcacaaac aggtaacggt
      121 tattgcaggt gtatttctta tctatgggtt taacatggat tttatcatta aaatcatgag
      181 tattgtccga gagtgattgg tcttgcgtat ggttaaccct aaagttatgg aaataagact
      241 tagaagcaaa cttaagagtg tgttgatagt gcattatctt aaaattttgt ataataggaa
      301 ttgaagttaa attagatgct aaaaatttgt aattaagaag gagggattcg tcatgttggt
      361 attccaaatg cgttatgtag ataaaacatc tactgttttg aaacagacta aaaacagtga
      421 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      481 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      541 atcacttcag gagtgataac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      601 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      661 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      721 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      781 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      841 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      901 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      961 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
     1021 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
     1081 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
     1141 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
     1201 caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta
     1261 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa
     1321 tttggaaagt tacacgttac taaagggaat gtagataaat tattaggtat agggcacctc
     1381 taataactac caattaattc acctcaaaat gaaattagaa aaaaacagcg aaatttttaa
     1441 atctatttct tatcgataca aattcctcgt aggcgctcgg gacccctatc tagcgaactt
     1501 ttagaaaaga tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta
     1561 gatgtctaaa aagcttgtag ttaaagcttt ttagacatct aaatctaggt actaaaacaa
     1621 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     1681 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     1741 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     1801 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     1861 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     1921 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     1981 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     2041 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     2101 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     2161 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
     2221 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
     2281 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
     2341 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
     2401 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     2461 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     2521 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     2581 ttgaaaccac aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
     2641 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
     2701 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagataacc
     2761 cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa
     2821 aaaaacgcat atagtagtct caaaactccc aagaa
//
LOCUS       DARDHD010000222         2852 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000222 DARDHD010000000
VERSION     DARDHD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.225"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1732)
                     /locus_tag="SDA06_002606"
     CDS             complement(<1..1732)
                     /locus_tag="SDA06_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229847.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSE"
     gene            complement(1692..>2852)
                     /locus_tag="SDA06_002607"
     CDS             complement(1692..>2852)
                     /locus_tag="SDA06_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229848.1"
                     /translation="EYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPF
                     VLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMH
                     LFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVL
                     EQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQ
                     DTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSG
                     GEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSS
                     IKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT          927 a          540 c          407 g          978 t
ORIGIN      
        1 tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact
       61 cattatgagt accttgatca actagttttc cttgatccat cactacaatt ttatttacct
      121 tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat
      181 ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta
      241 aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc
      301 ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt
      361 caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt
      421 gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa
      481 aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat
      541 caattaaaat acagccttca tcataatcat acagtctaga cactaatttc aagatactag
      601 ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag
      661 agatatgatc taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa
      721 tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc
      781 taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat
      841 cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca
      901 cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg
      961 agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc
     1021 tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc
     1081 tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag
     1141 tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa
     1201 aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca
     1261 tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca
     1321 tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct gataaattat
     1381 gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc actcttaaat
     1441 gcgcactttc tttataagta ctattatata agttttcata ctccctatct aacaaccaat
     1501 acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc tttgtatttt
     1561 ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt
     1621 atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga tcacttaaag
     1681 caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa
     1741 ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg atttccacgt
     1801 tctactattt tcccttctga caagacaaga atttcatcta cagatttaat gctggataat
     1861 cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt
     1921 tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca
     1981 tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact
     2041 cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa
     2101 ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa
     2161 acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg
     2221 ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc
     2281 ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat
     2341 tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca
     2401 cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt
     2461 gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt
     2521 tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa
     2581 acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga
     2641 gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa
     2701 aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac
     2761 ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat
     2821 atctttataa cttgaattcc gcggatatat tc
//
LOCUS       DARDHD010000223         2820 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000223 DARDHD010000000
VERSION     DARDHD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.226"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..415
                     /locus_tag="SDA06_002608"
     CDS             44..415
                     /locus_tag="SDA06_002608"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4229849.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            595..1017
                     /locus_tag="SDA06_002609"
     CDS             595..1017
                     /locus_tag="SDA06_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229850.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            1162..1407
                     /locus_tag="SDA06_002610"
                     /pseudo
     CDS             1162..1407
                     /locus_tag="SDA06_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1516..2472
                     /locus_tag="SDA06_002611"
     CDS             1516..2472
                     /locus_tag="SDA06_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229851.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
BASE COUNT          872 a          478 c          642 g          828 t
ORIGIN      
        1 atttagatta tgatagagaa aatacgtgag agggggacct gggatgtggc tatctgatga
       61 aaaaattcag tggcgtagct atcagaagcg tatattgact aactttgcta cttatttaaa
      121 taacaattca gtccatctag tggctccacc aggggctggc aagacctttt taggaattga
      181 gatgatccga cgtttggatt gtaaaacctt aattttggtt ccttccctaa tattgaagaa
      241 acagtggtta acaacaatcg aggaatattt cttagtaaat ccagcagata gttgctatct
      301 ttctggggcg attgaacatc ctagtcatat tacagtggaa acgtatcaga ccatttacaa
      361 ccgactgaag gaggagccaa cttatggtga tcagctggag ctgttagttt tttgatgaag
      421 ctcatcattt aaaaaaaagc tggggtgaat tgttgattga cttgaagaaa aaacaaccga
      481 atctgttgac gatttccttg acagccacgc ctccgattga tagctctcag acggagtgga
      541 agcaatatat tgagttaaaa tggcctgatt gacgaagaaa ttagcattcc cgagttggta
      601 aaggaggagg ttctatgtcc tcatcaggat tttctatatt tgattccggc taatcaggaa
      661 actactcagc attattttac ctttttagag aaacaaaatg agctggtgga agcgttaatg
      721 gaaagccaag agacggtgga ttatttgctt tctttagaat ttgttcagca tccgcttgag
      781 cattcgaagg aaatttatga aaattttgag ctgttcacta gtgcattaat ttacttgaat
      841 catcataact atgaattgat acccgaacat tggcagctat taggcagttc taagcagcaa
      901 ggtaaaaaag cttcagttcc atctttaggt aaaaaagagc tgatcactat ttatcagcag
      961 atgtttgaag atcaaccgga attgcctatt ttttatcaat tgaaaaaagc tggatgattg
     1021 tttgatgagt cgctgcattt gtttccagag tatccagaca ctgttggtag tcaggcgatt
     1081 cctgagaaaa agcaagcagt ttgtcaaatt ttgttaaaag aagaagttca gctgaagcaa
     1141 gaactgacag cggtcatttt gatggatcgt atttgcaagg aggtattagc aggcagtgat
     1201 ggtaccttag cttatggcgt ggttcctttg tttgatgaag ttgttcgtct attaggtaac
     1261 actcagcaaa tcggtgccat ctgtggagag ttcttactgt taccgaaaat gctagtaact
     1321 gttgagctgg cagaactagc gacgggtatg aatgaacatc cttatatgtc gggttatttt
     1381 tatttgacgg tggatgacaa aaaccgccaa gcattgatgc aagcagttac tcgcctatta
     1441 accgaaaaga agttgaatat tttggtagga accgttagtt tgctaggaga aggctgggat
     1501 tgtcctgcaa ttaatctgtt gatcttggct aatcaagctt ccacttttgt gcaaacacaa
     1561 caactacgag ggcggggctt acgcctaggt gcacaaaata aagtaacgaa catttggcat
     1621 ttagcgattg gattaccggg agttcctctg tctgaacagc ctgatttgaa aagaatgcta
     1681 cggcgtttgg gcaatatttc aggtctgacc tttgaatctt caccaaaaat tgagagtggt
     1741 ggcgatcgct ttgctttacc actggaatat acgaccacca cgatggaaac ttatactcag
     1801 tcgatgttct atttttcttg tgaacgaagt cggttgaaag aacaatggag tactgcttta
     1861 gctgatggct ggcacctatc tatgccaatc gtcgttagga aagtcgatca ggtaaagcaa
     1921 aaacaagaaa cttcattgcc gatagtagta aaaaatatag agcctctaag cttctggtca
     1981 gctatattta aaggtaactt gctggaatat ttccaaattc gtaggcgaca aaagctatgg
     2041 gaaaaggaat gtgcccgcaa aaaaagcttg gccatatgcg ttcaaacgac gttgcagcgc
     2101 cttggtgaag ttagtgatag aaaagtaatc gtagcggctg atcagcagga atttagatgc
     2161 tatcttgaga atgctggcta tcgagaagga cagcttttta ctgaagctat gagggagctt
     2221 ttagcaccgc tggagaatcc gcggtattta atcgaaggga aaaagatagt tctgacggta
     2281 ccagaacgct ttggccgaaa taagcaaatc gctaaccttt ttctaaacga attaaatcaa
     2341 tcgtcgctgc ccaaatgtca gttggtgtat actagaaatc ctgaaggacg ccaaaagcta
     2401 gtgacttatc gaatgaggca agtcttccag acccgcgatg aagtaattat tcaaaaaaac
     2461 atttggacgt aagttgatga aaataaattt tgctagagta gaacaagtcc gagaattaag
     2521 ttgctactta actgataatg ccaaggacag gagaatcttt tcttgacgac tgaaagaaac
     2581 tatttgactt atataacggt gtgtgatgaa tatttgcaag gcattctctt aaaaagtact
     2641 cgtaatagtt tttttgaaaa caaaacagta ctttattaat aggactgaca gagcgatata
     2701 agaacatctg cagacagagg tgaatgcttt tctcatgatc gatgaaacaa tggtacttcg
     2761 gatattcttg cttttagttt gaaccgttgt gattttcttt gattcctaaa agtagtttta
//
LOCUS       DARDHD010000224         2803 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000224 DARDHD010000000
VERSION     DARDHD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.227"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..466
                     /locus_tag="SDA06_002612"
     CDS             71..466
                     /locus_tag="SDA06_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229852.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVVVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            791..1408
                     /locus_tag="SDA06_002613"
     CDS             791..1408
                     /locus_tag="SDA06_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229853.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1449..1748
                     /locus_tag="SDA06_002614"
     CDS             1449..1748
                     /locus_tag="SDA06_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG4229854.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1782..1949
                     /locus_tag="SDA06_002615"
     CDS             1782..1949
                     /locus_tag="SDA06_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4229855.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1994..2599)
                     /locus_tag="SDA06_002616"
     CDS             complement(1994..2599)
                     /locus_tag="SDA06_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229856.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          986 a          417 c          509 g          891 t
ORIGIN      
        1 actatttaaa cagtgtcctt ttccttttta aatatatacg ataaaataag tatatgaatt
       61 gtaaaaggac atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa
      121 aaataattct ggatgtggaa ttatctttat gtttgttgta gttttgggaa tagtagtact
      181 tttaataact gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag
      241 aaaacttcct gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt
      301 aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga
      361 cttcaaagaa aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt
      421 tatttttaac cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa
      481 ttgtaaaagt taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat
      541 aaaagaaaaa ctggataact agtttattta atggcagacc gttaaggcct gccattttta
      601 tgtattttag cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaagta
      661 tactttttga catgccttga ttttctttgt ttatgtatgg cataataacc acataataca
      721 atttgaaacg tttctgattt aatctgattg ttttgaaaca attttagtga aaaaagggta
      781 gataaaaacg atgagaaaat ttttttatat ccgggtcagt agttatgatc aatcgatcga
      841 acgacaactg atttccgcga aggaattagg aatccctgaa gaatatatat ttgtagaaaa
      901 agccagcggc aaagatttta aacgccctga atatcaatta atgaaacgga tgtttcgaaa
      961 aggagattta ctctatctac aatcattaga tagactaggc agaaataaac aaatgatttt
     1021 agaagaatgg cgagaattaa ctaaggaaaa gcagattgat atttctgtgt tagacatgcc
     1081 tctgttagat actgctagtt acaagcaact agatgggtta gagacgctta tctctgactt
     1141 aattctgcaa ttactcagct atatggccga agacgagcgg aaacgaatta atagccggca
     1201 aagagaaggc gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc
     1261 tttacctcct gaatttcctg cgatctattc tgactggaaa gcaggaaaga taactgcagt
     1321 ctatgctatg gatcaattgg gcatgaaacg aaataccttt tatcgacggg tgaaagaata
     1381 tgaacatacc ttgcaccact tttcttgata gatataacag ctattagacg ggaagggaga
     1441 gtttgaaaat ggacttaacg aatcaaaggg tcgtttttac tggaacgctg cagcaaatga
     1501 cacgaaccca agccatcggt ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta
     1561 cttcaaaaac aaatttcctg gtttgtggtc agtattctaa gtcgttgttt gatgattctc
     1621 tctatacaaa aaaggaacaa accgcgcggg acttgattgc gttaggtcat gagattacca
     1681 ttttatcaga agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt
     1741 atttataatc gttagtaaaa tttacaagga ggaaaatttt tatgaagcga aaatcttatg
     1801 atcgttgggg cattgaaata actagtttta atcgaaatat tgcttttcac gtggagtgtg
     1861 actgtgggga attagcaaaa agtgtttatc aaaaaaatta cttttactgt cctaaatgcc
     1921 atcgccgata cggggtacat ggaacataga aacgaaatag tgcggtagaa aagcgaatga
     1981 atgtagtata tttctacaaa tcattcgctt tctttatttt ataaatagtt ggtcgagaga
     2041 tcccgttttc ttttgcaatc tctgcaactg gaatgccttg ctttaacatg tccaccacgg
     2101 ttttaaaaac taagcgtttt tgcggatctg ttgagttaat agaatactcg attggtcgtc
     2161 ccttatatcg gccggctttt tgagccaata atactccttg ccgctgacgt tcacgaattt
     2221 tctcacgctc gttttgtgca atataactta gcagtgataa aaacatatcg aacatagcgg
     2281 tatccaggag ttcatttcca gtattaaaat ctaaaaactg tgcatctaat atttttaacg
     2341 ctactttttt ctgtttcata aaagcaactg tattttttat atcttcataa tctcttccta
     2401 aacgatctag tgatgtaacg atgacgcaat caccttcacg gatataattt agtaattttt
     2461 gaaactcagg acgctcagta tctttaccac tggctttttc ttggaataat ttatcaatgt
     2521 taagtccttc catcattgcc aattgtcgat ccaaattttg atcttgtgaa gatactcgaa
     2581 tatagccaat ttttcccata aaaaactcct ttttaaaagt gatataattt acatgtaaaa
     2641 tttacttgta gttttactgc tatcatagca tttttaaaaa taaattttat gtagacttaa
     2701 aatttacttc atggagggtt taaatggatg aaatcaaaag taacaaaaaa gcatttggca
     2761 tttatttagg agaacctagt acagaacaat taaaccaatt ttt
//
LOCUS       DARDHD010000225         2785 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000225 DARDHD010000000
VERSION     DARDHD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.228"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..845
                     /locus_tag="SDA06_002617"
     CDS             156..845
                     /locus_tag="SDA06_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4229857.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            859..2361
                     /locus_tag="SDA06_002618"
     CDS             859..2361
                     /locus_tag="SDA06_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4229858.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2374..>2785
                     /locus_tag="SDA06_002619"
     CDS             2374..>2785
                     /locus_tag="SDA06_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4229859.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQF"
BASE COUNT          893 a          390 c          546 g          956 t
ORIGIN      
        1 ctcctctaca atttcaactt ttctccatta tactccaaaa taataaaaaa aactcctaaa
       61 tactccattt ttatctttac ttttatgaaa gcggatgcta tattgttttt ggagttgtta
      121 tccaaaaata ataaaaaatt ggagtgttta ggataatgga taaaaagagg tttttagatc
      181 ttatttcaaa aggtttgatt gtttcctgtc aagcattacc tggtgaacca ttttacaaag
      241 aggaagaaag tttaatgcct tattttgctt tggctgctca aagggctgga gcagttggta
      301 tacgtgcaaa ttctgttcga gatattattg aaattagaaa gtgtgtatca ttaccaatga
      361 ttggaattat taaaagagat tatccaccag aatctccatt cattactgcc actatgagag
      421 aagtagatga attagtagaa accggtgttg aggtaatcgc tcttgattgc actcttagaa
      481 atcgacatga tggactatct ataaattctt ttattactaa aataaaagaa aaatatcctc
      541 atcagttatt tatggcagat atttctacat ttgaagaagg caaaaatgca tataaagcag
      601 gaatagattt tgtaggaact actttaagtg gatatactga ggaaagtaaa aaacaagatg
      661 ggccagatat agatttgatt aaaaaattgg tagaagaagg gatttgtgta attgctgagg
      721 gaaggattca tactccagaa caagcaaaag agataaaaaa aataggagtt gcaggtattg
      781 ttgtgggtgg tgctattact cgaccacagg aaattgcatc aagatttgtt aaagcattaa
      841 gttaggggga gaaataaaat gtttaaaaaa ttttcacaat taggacgcgc tttcatgtta
      901 ccaattgcta tattaccagt agccggatta ctattgggac tgggaggagc gttaacaaat
      961 gagagtgcta taaatgctta tccattttta gatcaaccgt ggttgcatac aattttaagt
     1021 attatgagtt atgctggaaa tgcagttttt gcaaacctag cactaatttt tgcaattggt
     1081 attgctgtag gacttgcaaa tggagataaa ggaacagctg gtttagctgg tggagtagct
     1141 tatctagtat atactgcaac tattagtggg tttcttgaac ttttttcagc aaaagatgca
     1201 acactagata caggtgtagt gggatcaatt gttgttggtg gtactgttgc atttttacac
     1261 aatcgttata gaaaaattga attaccacaa ttcttaggtt tttttggagg atcaagattt
     1321 attccgattg tttctagttt agctgcaatc attattggtt ctttctttta tttaatttgg
     1381 ccaccaattc aaaatggttt agtagttgct ggtgaacaaa ttgcccaaat gggtagttta
     1441 ggaacttttt tatatggttt tttattgcgt ttaacaggtg ctgttggact acaccacacc
     1501 atttaccctt tgttctggta tacatcttta ggtggtaccg aaactgtagc tggatcaaca
     1561 attgctggag cccaaaatat tttctttgcc caactagctg atccaaacca cactggtctg
     1621 tttacttatg gtactagatt ttttgcagga agatttgcaa caatgatgtt tggattacca
     1681 gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa atcgtaaaaa gaatggtggg
     1741 ctttatttta gcggggccct tacatcattt ttaactggaa ttactgaacc agttgaatat
     1801 atgtttttat ttgttgctcc ttggctttat gtgattcatg cattcttaga tggattatct
     1861 ttttattttg ctgatatctt aaatattcgt gtagggaatt ccttttcagg tggtttgatc
     1921 gactttttat tattcggtgt acttcaaggg aatgacaaaa ctaattggtt aaaattaatt
     1981 ccttttggta ttgcatgggc atttatatat ttctttgttt ttacattctt tatcaaaaaa
     2041 tttaaggtag ctattcctgg aatggaagaa gaatcagaag ttcattctga aaaagtaata
     2101 aacaaaaata catcattaca tgaagagtct cttatcatta ttgaagctct tggtggaaaa
     2161 gaaaatattg agtctgttga agcttgtgca actcgtttgc gtgttgctgt tacgaatggg
     2221 gagattattc aaaaacaaac aataaaagac actggtgcaa ctgctgtctt cgaagtgaag
     2281 ggtggcatcc aagctgtttt tggagggaaa gctgacttat taagtcaaga aatcaatcaa
     2341 atattaggaa aagataatta ggaggttttt aatatgtttg aaattttcaa aaagaaaaaa
     2401 caagaaactt tttatgcacc ttgttccgga aaaatcattt ccatcacaga agtaaatgat
     2461 cctgtctttt cacaaaaatt actaggtgat ggttttgccg tcatacctga tggttcaaaa
     2521 atttatgctc cattggctgg tcgagtaaca aatatatttc ctactaaaca tgctattaat
     2581 ataaaaagca actcaggcat tgattatttg attcatattg gaattgatac agttgaatta
     2641 caaggagagc ctttttcgat ttgtgttcaa gaaaatgatg aggttactcc tgaaacctcg
     2701 ttggttgatg tggattttga caaaataata aaatctggta aagatacttc tgttatagtt
     2761 gtttttccgg agaaaaatca gttca
//
LOCUS       DARDHD010000226         2740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000226 DARDHD010000000
VERSION     DARDHD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.229"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..171
                     /locus_tag="SDA06_002620"
     CDS             <1..171
                     /locus_tag="SDA06_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229860.1"
                     /translation="DLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDGKKAQQ
                     HIETVKKILDIR"
     gene            183..431
                     /locus_tag="SDA06_002621"
     CDS             183..431
                     /locus_tag="SDA06_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229861.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            462..641
                     /locus_tag="SDA06_002622"
     CDS             462..641
                     /locus_tag="SDA06_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229862.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(684..1154)
                     /locus_tag="SDA06_002623"
     CDS             complement(684..1154)
                     /locus_tag="SDA06_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229863.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1241..1939
                     /locus_tag="SDA06_002624"
     CDS             1241..1939
                     /locus_tag="SDA06_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG4229864.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2117..2458
                     /locus_tag="SDA06_002625"
     CDS             2117..2458
                     /locus_tag="SDA06_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229865.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2451..>2740
                     /locus_tag="SDA06_002626"
     CDS             2451..>2740
                     /locus_tag="SDA06_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4229866.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1011 a          399 c          555 g          775 t
ORIGIN      
        1 gacttaaaaa ttcgagcgga gatgagaaaa agaagaatga ctttcaaaga actagctgcg
       61 cttatcggta tttcaggagc ttatttatca gatattctaa acggcaatcg tgatggaaag
      121 aaagcacaac agcatatcga aacagtgaaa aaaatattgg acatccgata ggggggtagg
      181 cgatgacaaa actaaaaaaa caagattttg taaaaaaata caattattct ccatctactt
      241 atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg tatgaacggg
      301 tcacaggaca agaagtttgg atcaatacag aattatacga taaatttttg tctttcaaat
      361 cctataacag gttacgcaca agaaaggtaa cacctaaaga atttatcgag aagcatttag
      421 ttgatttata aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa ttcaacagag
      481 cattggtatt cagcgcaccg ctaatcatct acgctttagg actttgggga agcaggcaag
      541 cgttgatagg aacgattgtt tacatggttt ggatttttat ggggcttgat gaagctgaag
      601 ctgagtacag agcgaaaaag ccaaccgagg gggctgacta aaaatgaata gaaaagagaa
      661 actagaatgt atattactat tactcagttt aattctttca ctaatttctc tattgggaag
      721 tttgtatttt tgatatcaac aattttgtgg atttgggttc tatttagagt aaatatcatt
      781 tttgtttggt gattaaggag tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa
      841 caagatatag cacttttagg aggaatattt agcggtacag cttcagagta tattctatta
      901 tcatcgactg atcctccttt agcaattaat ttcttaaatg gagtagctga ccatctgcgg
      961 tttaagttag cggatgaaag ttctaaatta actaacatag ctggttctgt tgaatagtta
     1021 gaaatgatta cttttgtata aatcatatct gcagcgaagt aagaagcatt aagttcaact
     1081 tctagttgag ggcgcattcc ttttattttt atccctgtta aaaacgtact caatataaaa
     1141 cttattattg acatccattg aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc
     1201 agtttttaac taaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt
     1261 gaaaaataaa gaagctgtga ctacgagctt acaagtggca gaaagcttcg agaaaaaaca
     1321 tcaacatgtt ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat
     1381 gtttgtagaa ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag
     1441 tagagatgga tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa
     1501 acaaaaatac attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc
     1561 tcaaacaatt gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga
     1621 tgtttccatg cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca
     1681 aaaaggaaat atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga
     1741 aatcagcaaa aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc
     1801 aagatatggc gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg
     1861 gttgccagaa actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt
     1921 tggtgatgac aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct
     1981 ggtggaaata ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat
     2041 ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat
     2101 aaggagattt tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc
     2161 tgattggggc aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt
     2221 gacgaatgaa gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga
     2281 tcatgtttat cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat
     2341 cgaagatggc ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc
     2401 ttattacgga ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa
     2461 tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga
     2521 aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg
     2581 atccacaggc aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc
     2641 atcaagactt tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg
     2701 tatctgtctc agtaacggtt aaaggacaca cggaaaccga
//
LOCUS       DARDHD010000227         2695 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000227 DARDHD010000000
VERSION     DARDHD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.230"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(75..1169)
                     /locus_tag="SDA06_002627"
     CDS             complement(75..1169)
                     /locus_tag="SDA06_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4229867.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="SDA06_002628"
     CDS             1358..2119
                     /locus_tag="SDA06_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4229868.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="SDA06_002629"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="SDA06_002629"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4229869.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          853 a          539 c          462 g          841 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcagg
//
LOCUS       DARDHD010000228         2690 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000228 DARDHD010000000
VERSION     DARDHD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.231"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..111)
                     /locus_tag="SDA06_002630"
     CDS             complement(<1..111)
                     /locus_tag="SDA06_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4229870.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQT"
     gene            181..531
                     /locus_tag="SDA06_002631"
     CDS             181..531
                     /locus_tag="SDA06_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4229871.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            945..1763
                     /locus_tag="SDA06_002632"
     CDS             945..1763
                     /locus_tag="SDA06_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4229872.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1836..2195)
                     /locus_tag="SDA06_002633"
     CDS             complement(1836..2195)
                     /locus_tag="SDA06_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4229873.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          889 a          393 c          487 g          921 t
ORIGIN      
        1 tgtttggcgt ccacgtttaa gctgcttcgc aacgatggca accggagttt cttgatgaaa
       61 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
      121 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gcttttttat
      181 ttgtctcgct taattataca aacggcgttt tataaagcaa tcattcctga gaacttagat
      241 gaggttaaac tttatttatg ggaagataac ggagaaattg tcggttttag tggtgtaagt
      301 gcagaagaat tagttatgct atttcttgat ccagatttta ttggtaagcg atatggtagt
      361 caaatattaa ttgagctgat tgaaactgaa gatataaaaa gaattgatgt taatagtcaa
      421 aataaatatg caaaacattt ttatttaagc catggatttg aaattgaatc tgaagaagac
      481 ttagatggtt ttggtaaacc ataccctata acgcatttag taaaaaaata attcatgaat
      541 ggtttattac ctgattgaac atgaataaaa gtaatatttt tattttgatg gcctatttct
      601 aggttatgaa ttatccagat caaatttgtg tattacattc agtaataagg tgcttatctt
      661 aggatcaact tatgatacta gctgaccaag gatgtaccag ttatagaaaa tcagttaaag
      721 taaaaatgat aaaaatctta tgtcaacact gatttaggct gacctgtggg ttaggagaaa
      781 gacaggtcgg tagcttttaa tacaaaaaat ggcttgtatg tatctagata gttaactgaa
      841 attagaaatg ttcttatttt taacggagta agaaaaatag gctgctttag gtctgaatac
      901 tttttcaata taatgaagat atcaaaggcg acaggagtgt ttttatgaat aagaatcctc
      961 ataatctaaa gttggtagca gtagatatgg atggaacatt tgctcgtact gattacacat
     1021 acgatatccc aaggttcaag gctgttttat cgcgaatgaa agctgttgat tgtcaatttg
     1081 ttgttgcaag tggaaatcaa tactaccaat tgagatcgtt atttccaaat tattatgatg
     1141 aattaagttt tgttgctgaa aatggtgctt ttgttaaaga taaaacagag gtactatttt
     1201 cagccgatat gcctagaaaa gcgatcgagg tggtccttga tttatgtaaa gactatcctg
     1261 aagtaaaaaa tgtgatgtgt ggtctgaata gtgcctactg tgaaagagga aaggtgagtc
     1321 aagcattttt tgatctaacc aatatctatt accatagatt agaatgggta gctgattttg
     1381 cagaagttca agataaaatt ttaaaatttg ctccgactat gcctgttgaa aaaaccgaat
     1441 tttacgttga attgttccgt gaaaaattag ccgggctatt agtacctacc agtagtggtc
     1501 atggttctgt agacttgatt ctttctgagt gccataaaac tttcggcttg aaaatgttag
     1561 ttgagcgctt ggggattaat cctgaccagt gcgttgcttt tggagatggg ggcaatgaca
     1621 ttgagatgtt ggagtattgt ggtctgagtt atgaaatgga taatgcaacg gaagctgtta
     1681 agcaagttgc taagcatcaa tgtccttcaa atgacgaaga cggtgtttta gtgacgctcg
     1741 atcgattgtt ccctaacacg taaacactag gtaattcatc aaaaaacctc gatataagaa
     1801 atattcgagg ttttaaaact atatttagaa cagatttacg aagcgtctga tgagtcaaaa
     1861 tagacttgga cattgctatc taaaatacca ctttcattct ttaaacgaat tgtttgattt
     1921 gatggagtta cctctaatac taaatgccca ttaataaagg tagtatcgta ttcagatgtt
     1981 ttaactgttg cttcttcgca aggtattgtt aaatcatatg ctggaatata atgttgtgtg
     2041 gaaactaata aaaaatgatc accaatcata cagcatccct ctgctgaaga aaattgatta
     2101 gagccatcat ttaaagttaa caagaatcca tcctttaaac gaggtacttg cttaaatact
     2161 tctggttgta cttctaaaaa taaattatcg ctcattttca cgaccaccat cgacttccac
     2221 agttgaaatt gacattttat ttaacacacc tattccccct taactataac tcaattctat
     2281 ataaaaaata ttctattctc aaataattga acttttcaaa tcgccaattt acttcggctt
     2341 tatcagagct cattcatatc aagagcgatt aacgctgtaa aaattaaacc gaagatctga
     2401 taaattttct ctattttcta caatatttaa aacgcttcct tagtctatta tatatctatt
     2461 tctcttagga gtatgtttag atgtttgaat aatagttgcc caactttaaa ggttattctt
     2521 ataatatttt acttcatttt ccaatagaaa attcaaagga ttactggttt ttctaaaata
     2581 gtttagttta aatacataag tatcctccga cactgtatta gttcataatc atctaaatac
     2641 aaaattttca aataaaaata tttcaagctc tatcgttgac gaatgtaaac
//
LOCUS       DARDHD010000229         2648 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000229 DARDHD010000000
VERSION     DARDHD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.232"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            257..532
                     /locus_tag="SDA06_002634"
     CDS             257..532
                     /locus_tag="SDA06_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229874.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="SDA06_002635"
     CDS             545..2617
                     /locus_tag="SDA06_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG4229875.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
BASE COUNT          809 a          413 c          569 g          857 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttata
//
LOCUS       DARDHD010000230         2644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000230 DARDHD010000000
VERSION     DARDHD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.233"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            124..1209
                     /gene="xerS"
                     /locus_tag="SDA06_002636"
     CDS             124..1209
                     /gene="xerS"
                     /locus_tag="SDA06_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG4229876.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1493..2158)
                     /locus_tag="SDA06_002637"
     CDS             complement(1493..2158)
                     /locus_tag="SDA06_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4229877.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(2312..2461)
                     /locus_tag="SDA06_002638"
     CDS             complement(2312..2461)
                     /locus_tag="SDA06_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG4229878.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(2454..>2644)
                     /locus_tag="SDA06_002639"
     CDS             complement(2454..>2644)
                     /locus_tag="SDA06_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG4229879.1"
                     /translation="ELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLGLVPVESV
                     EEVLGMKIEVEEDFQNHG"
BASE COUNT          907 a          465 c          376 g          896 t
ORIGIN      
        1 atatgctttt tttgaaacaa attaataaat tttagagcat accaattgac aaaacattct
       61 ataattggta tagtccaatt atagagatac tcttctcccc tacttattga aaggatgaat
      121 gacatgaaaa atacagaaat atatcaaaaa atagatacaa tcatacttca tatgcccgat
      181 tatataaaag attttgttca agatcgtgag gataaagatc aatcacctcg aaccacttta
      241 gaatatctaa aaaattataa gctcttttat gaatggcttc tgagtgagtc tatcgttcct
      301 gatacttcta tcacctcgat aagacagtta actgcctatg acctaaactt gtacaaatct
      361 catttgaaac gtcgcacaaa agaaaatgta aaaaaagaga caacaaaaac taagctcaaa
      421 gataataata acttaggtct atctacttct actatcaata gaaatatcac ggcattaaag
      481 gttctattta aatatctttc gaaaagttcc aataatcctc taggaaaacc atacttagac
      541 aataatccta tggatcaagt agcaaccatc acagataaaa ctacgttagc tgcacgtgcc
      601 aatgccattg agaaaaaatt atttctagat aaggatacac aaaattatct agattatatt
      661 gccaatgaat ataaaaatac tttgagtaaa cgagctttga tctattatca acgagatgta
      721 gaaagagacc tggcgatcaa tgcactgatt ttaggatctg gattgagact ctccgaggtt
      781 ataaatatta atttagatga cctatcttta gataagaata atgtggtagt aactagaaaa
      841 ggaaataaaa gagatgctgt caatatagca gcattcgcaa tggaatattt agctaattat
      901 ctagctattc ggaaagaacg atataaagta acagaaaacg aaaaagcttt atttctagca
      961 atttaccaag gagaagccaa gcgtatatcc ggtattgcaa tcgaacggat ggtagcaaaa
     1021 tattcaaaag gctttagaat tcaagtcagc cctcacaaac tgcgtcatac attagctact
     1081 agactctacc aacagacaaa ttcattggta ttaactgccc aacaattggg acattcaagt
     1141 accaatacaa caactttata tacccatata gacaatgcgg ctacgatcga tgcattaaat
     1201 gctttatgag tataatgtat aaataggcgg aaataagaaa caacacgcta gaaaagcctg
     1261 tattatagtt tttctagtgt gtttcttttg gtgtgatcct acttttgttt atcatttatt
     1321 ttttattatc gtaaataggt gtcggtttga ttttagaaaa attagtttgg atataatctg
     1381 tgggatcatt tgttatctct ctcaatgaat ttagatacaa atgaccataa gttcctaaag
     1441 tcgtttgtac atcagcatgt tttaaccttt ctttaattcg aagtgcatct ttttattgtg
     1501 atataagcaa agaagcatga ctatgcctta atccgtgtat gcggattata tgaacaccaa
     1561 cgatcgttgc atgtcttctc ataatatgac ggattgtata tttctgagta ggtgttccat
     1621 tataagaaag tacaaacgac atgcctccta ttgaaacttg acgctccttc caagctttta
     1681 aaaaggtaat tgtattgtcg tctagttcga ttgtacgaat actagaagcc gtttcgggtt
     1741 ctacaaactc ataggttttg gctctcttat aatagagtgt tttattcaca cgcaagtttt
     1801 tattttcaaa atcaatatct ttccaagtta atgcagtggc ttctcccaaa cgcataacgg
     1861 tcataaaaag taaccacata caaataaaac caaaatgttg gtacaaatca tcaatataaa
     1921 aagttgaaat aacttttgta aattcttctt ttgtccaaaa atcaactgta gttttccttt
     1981 tcttcacatt tccaataact tttgcaggat ttttttcaag tagtcccaaa acaactgctc
     2041 gatctaatga catctgaaaa atcccataca tgtttcttac ataagaattt ttatatttgc
     2101 tcaataaatt attttgccac ttttgaatgt caattggacg tatttctctt aactccattt
     2161 taaagaaata agaaaagttt ttttgattgt agataaccga tttttatatg ttctttcttc
     2221 tactttccct tgataataag gtaggaataa tttataaaaa aactcctcaa acaacatagt
     2281 actttgagaa gtttctgctt cttttccata attagaaaca agttcggtat acgctttttt
     2341 tcttctttaa ctgttttaac ccctctacgt ttttttgtac tcttttccct gttcgtttgt
     2401 cgaatcccaa actagcagta aaatgatatt tcccattttt atctttttta aaattaacca
     2461 tgattctgaa aatcctcctc tacctctatc ttcataccta aaacttcttc tacagattca
     2521 acaggtacta gtcctaatct tctattttcg taataggtat agctttttga aaccataatt
     2581 gcttttgcct gtcgtatgat tctctcagac tgatacttcc cataacctaa tttcattaat
     2641 tcat
//
LOCUS       DARDHD010000231         2630 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000231 DARDHD010000000
VERSION     DARDHD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.234"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1152
                     /locus_tag="SDA06_002640"
     CDS             <1..1152
                     /locus_tag="SDA06_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEG4229880.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="SDA06_002641"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="SDA06_002641"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG4229881.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          871 a          494 c          626 g          639 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa
//
LOCUS       DARDHD010000232         2575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000232 DARDHD010000000
VERSION     DARDHD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.235"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1437)
                     /locus_tag="SDA06_002642"
     CDS             complement(<1..1437)
                     /locus_tag="SDA06_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4229882.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKL"
     gene            complement(1456..1860)
                     /locus_tag="SDA06_002643"
     CDS             complement(1456..1860)
                     /locus_tag="SDA06_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG4229883.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(2092..2349)
                     /locus_tag="SDA06_002644"
     CDS             complement(2092..2349)
                     /locus_tag="SDA06_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG4229884.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          795 a          486 c          352 g          942 t
ORIGIN      
        1 taatttatcg tccgatgtta attggatttg ataaatccaa gaattcaaaa agatagacgt
       61 tatttgaaga taataatagt caaattctat ttgaaaatct ctctctattt ccttaacata
      121 ctgtctaaat cgaatctctt gataggtggg atatgttaaa gctgaattat tttggatatc
      181 aacattcttt tgtctaaaat atctatcata tttctcaaaa agctgctgct tatcagtgtc
      241 tttcttccac gtttgataaa gatactctat aagttcgttg atattaaaaa cttcgtctac
      301 tttttccaaa tcatccattt gaatatattt taattcattc tgtcttttca taaccttcat
      361 gccgttgaaa ttcacccatt gtggtaaatg atacatctga atataaagtt cttccaaatc
      421 atctaagaaa taaaaggcat tggcacaggt caattgaatg atatttttca aattgccaac
      481 attttcacta aagcgatagt ttaataaaaa atctaataat tgttgagata tcagtatttt
      541 ctttgctagt ctttcttctt ctgatttaaa aaatatcttg attaactgtt ttctttcata
      601 aagagaacga ctgtctaatg ttggaagtgt tagctttaca ggtattcttc tcaacaaagt
      661 tgataatagt ttatcctcag gtggttctga tgttgcaaat ataatccagc aacttgatgt
      721 gtaccagttt tcattatcac ccatccgatg aaatttcctc gtatccataa actgaaaaag
      781 tttctcctga cattcattgc tcaatgcatg aatttcatct aaaaataaga ctccattatt
      841 agctaattct gctaaaccct ttcttccatc ttcggctcct gtatatgcac cttttacatg
      901 cccaaaaaaa ttggacaaga aaagttctgg attattggca tattcactac aattaactga
      961 aatgaaagat gccttctctt ctatataatt attggccaac aaatccttat acacataatt
     1021 tgctaaatag ctttttccag ttccagtctc gccatgaagt aaaatgggta agccatttgg
     1081 cggatagtag attgctgctt taatttgagc aattacattt cgcagacttc cttgacttcc
     1141 gataaccctt tctattccaa ccctaaaaga gctatcatac catttttccg cttcctctga
     1201 agagctaaaa atttgttgat ttggaacttt ctccggtaaa gaaaagaaga ttactggacg
     1261 agtattgatt tttccaaatt ctccatcttt tacgaattca ttcaaatatt gagagacaat
     1321 attacgacta acatttaatt tttcagatat tttctgtgct gtattgttcc cccatttatt
     1381 tttaatctca gagttctcta tgacataatt gacaatgcgt tcttttaatt cttccatcac
     1441 agcacctcct cgacactatt cgaattttgc cgacagtatt tttccataaa tatgacttgc
     1501 taaaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     1561 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     1621 cagatcttct tttgattctt catctgaaag taattcacga tacaacatcg tttgtttgct
     1681 tactgtgtca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     1741 gtcggataaa tatttataga aaatcagccc caataaataa tttttatatt ctgacgcatc
     1801 cattttacta cggagattat ctgccgcact aaataacttc tgatttaatt ctgcacccaa
     1861 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     1921 agttttagaa aaagcaaaaa aataaagaaa cttattcttg ataacaagct acgtgacgta
     1981 tatccctaac acaaaaatag ccatatcctt tggtttggat acggctattt gatattactc
     2041 attcaattat caaatcacag taggtgtgaa tctcatcggg atcggcaata tctacttctg
     2101 attaggatca aaggtgattt tctcaataaa ggtgtgatac aaagctttaa cttgcttctt
     2161 ctcactctgg ataagaaagc aatccaaact agtcaatatc tgttggacat ctttgacttg
     2221 aatcggttcc ccttgttgtt gaaagatccc taagatttca ttctctcgaa tttgtaactg
     2281 tcttcgtttt tcagtcaatt catttaaacg atcttttaac atcggaaaca attcgggact
     2341 atcttccaaa gcagcttcct gtttttgctc taatagttag attttgcgta ccattttctt
     2401 cttttatttt ccttcgattg ctgattttaa tatagtctaa aggaaattta tatttattgt
     2461 attctttata acttcaagag aacttcgttt gagtattact gtattttttt aattccataa
     2521 gaaaattagt tattacctgc cacctaatca gcaaatctga acctgagtca atata
//
LOCUS       DARDHD010000233         2541 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000233 DARDHD010000000
VERSION     DARDHD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.236"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..175)
                     /locus_tag="SDA06_002645"
     CDS             complement(<1..175)
                     /locus_tag="SDA06_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229885.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITY"
     gene            complement(169..570)
                     /locus_tag="SDA06_002646"
     CDS             complement(169..570)
                     /locus_tag="SDA06_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4229886.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(742..2535)
                     /gene="uidA"
                     /locus_tag="SDA06_002647"
     CDS             complement(742..2535)
                     /gene="uidA"
                     /locus_tag="SDA06_002647"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG4229887.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          758 a          497 c          336 g          950 t
ORIGIN      
        1 cataggtaat aaattgcccc gcctcattaa atccatttgt caaacataac ttagctatat
       61 aactatgata atgtttgagt atcatcttga ttgaaattgg atcttcttct agagcagatt
      121 ggataatgga tagaggaact aatggatact gccgtttttt ggggttgtct accataagtc
      181 ataccccatt tttaattgtt caactgcctt ctttcgttga taccagatac cacttgtact
      241 catattgagg attttaccga tttcagtatc attaaaacct gcaaaataat aaagtaggat
      301 aacctttctt cttatttccg ataaatgatc aatggctgcc gataactttt gatctgaaat
      361 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggagta
      421 cttaccttca tttgaattcg tcaatggttc ctcattatct gttatgttaa tgtttcgttt
      481 tcgaatagac ttagtttcgt ttcgtaatac ttttttacag taggcatcaa attgatgttg
      541 tatagatttc tcattgtaat tttgttgcat gataaatatg ctcctttttt cccctttcca
      601 ctaatacaac aatcagaaag cgcctttttt tcgaaaaaac tagaaaaaaa tatccttctt
      661 taaacagaaa atctttttat aaaaaaaagg agttggcaat taagcagcca ctcctttttt
      721 atattcctta taaaaggtaa attatttttt ataactgaaa tttggtattg atgaccatcg
      781 tttctttaga tagtgagcaa tcattttagg ctttctatct ctagtaaaga ttcctttctt
      841 atttccttgc actcttaaaa tcccttgact tgtttcaaaa tctgcaaaat tccacacttg
      901 ttcaccaaca aaattgtcac atttatctat tacttcgtga tttgcttcgt aataacccac
      961 ttgatactct tctgtaaaca ttgttggtac tgtatcatga agtccagcta ctgtatctgc
     1021 tccatattct gtaaacatta ttggcgtatc gggacatctt tccatccatc catctagctc
     1081 ttttttcaac agatattttg catgttttag gtctccacct gaaacatacc aaccataata
     1141 gcgatttaaa cataaaacat cgacaatatc tcctacttta caaatatctg gtgttgactt
     1201 taagtaagta acgattgtta caggtctctt ctgagggtca agttctttag tcaattcaac
     1261 taaaggttca aaatattctt tagcgccttc agaatccgaa tctggttcat ttgcaataga
     1321 ccacattacg acactcggat ggttcttgtc gcgttctatc aattccttaa tcgccatttt
     1381 gtgagcattt tctgtccgta tttctttcca agtatcatgt ttttctacgt tgtcatctaa
     1441 taaagtagcc atgaaattca aatgtaatcc tactgcaggt acttcatcaa taactacaat
     1501 accttctcga tctgctaagc gcataatttc ttctgaataa ggataatgtg aagtcctaaa
     1561 tgaattagca ccgatccatt tcattaaatt aaaatctttg atatttactg cttcattcaa
     1621 accacgacca ttaatatacg tgtcctcatg cttcccaaat cccttaaagt aaaaaggttt
     1681 attattaatc aaaaacttcc cattcgaaac ctgaatacta cgaatgccaa attcttctgt
     1741 gtacgtatca atcactttat tttcgttaat taaactaact ttcatactat ataagtaagc
     1801 atttagaggc tcccaaagtg ttgggttctt aagttgaatc actccttctt tccccttagc
     1861 ttctgctata agttgagctt cttcgtcata gattgctatt agaatttctc cggtccattc
     1921 tgtatcgata ttaaattcta catttgcatc tgaatcaatt atttttgtat taatagtgac
     1981 atcctttata tagtctttcg gagttgtata caatttaacg ggtctattaa tgcctgcata
     2041 attgaaaaag tcaaaattaa ccaaattttc tttttttatg tctccatctt cctgtatcgt
     2101 ttctttcaaa atccctacag gtaaagtgct atcgtcaata acattgttta cagccactgt
     2161 aattctgtta cttccatctt ttataaactg attaatttct gattcaaatg gcgtaaaccc
     2221 tcctttgtgt tgattaatca attttccatt caaaaatact tttgcttcat gtgttgcaga
     2281 tccaaaacga agaactaatc tttcttctaa taatttttta ggaagaatca cttctttttc
     2341 ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga
     2401 agaaggaact gaaattggat aactctcttt ttcaaccatt ggttctaaag caagttcttc
     2461 ttgtaagtca tcatttgtat ctaacttaaa tctccaaaga ccgcttaaat caattacttg
     2521 cctcgtctct gtcataatgg g
//
LOCUS       DARDHD010000234         2491 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000234 DARDHD010000000
VERSION     DARDHD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.237"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(620..>2491)
                     /locus_tag="SDA06_002648"
     CDS             complement(620..>2491)
                     /locus_tag="SDA06_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4229888.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          717 a          498 c          394 g          882 t
ORIGIN      
        1 atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga cctgttaaat
       61 ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc aaaacaagcc
      121 cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg tatatttttt
      181 tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc ctactagtta
      241 gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt atctgaaaca
      301 gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt cagatacatt
      361 tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag cctatgcttt
      421 ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat gaatgaaaaa
      481 ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat gtgaacaaat
      541 aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg aatcgtacaa
      601 ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac tcatcatcta
      661 acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc gcttctggat
      721 gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata ttaggaattg
      781 gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca gaaacaaacc
      841 actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag cgacgtttaa
      901 tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt aaaatttgtc
      961 ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt tcaatttctt
     1021 ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata taagttgaat
     1081 caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa aaatcttctc
     1141 cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac acgccaattt
     1201 gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg tcatcattgt
     1261 aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat gctttgacgt
     1321 actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat gaaacctctt
     1381 ttaacttacc atatgcttgt ttgaatcttt ttttggattc ctgataactt ggataaatca
     1441 aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca ttcttctcca
     1501 ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga tacgtacgat
     1561 tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc acaaataggg
     1621 tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc gctaaatcga
     1681 tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta ttgatgtctc
     1741 cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga ttatattcct
     1801 caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt tcttctaatg
     1861 aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct tcacgaatga
     1921 gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct ttgatggccc
     1981 gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat ttcgttggcc
     2041 tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt ttatgaatga
     2101 ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt tcttttttga
     2161 caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct gcatatgttt
     2221 ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc acggtgtttt
     2281 catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta atggtatagg
     2341 tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct ttggcttgtt
     2401 tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat ttcggaaaca
     2461 tttttttgat ttcttccata ttttcagcac t
//
LOCUS       DARDHD010000235         2465 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000235 DARDHD010000000
VERSION     DARDHD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.238"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            206..595
                     /locus_tag="SDA06_002649"
     CDS             206..595
                     /locus_tag="SDA06_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEG4229889.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            771..1514
                     /locus_tag="SDA06_002650"
     CDS             771..1514
                     /locus_tag="SDA06_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229890.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1507..>2465
                     /locus_tag="SDA06_002651"
     CDS             1507..>2465
                     /locus_tag="SDA06_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG4229891.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTP"
BASE COUNT          851 a          379 c          464 g          771 t
ORIGIN      
        1 cctagacatc gatttgaacg cctattatag cggtatcgaa ctaggcgaaa tgcttagaga
       61 aaagaatgaa aagtgtaaaa ttgtttatct aacgtctcat ccgaataaag caataactgt
      121 tattaatcga aagattacgc ctagtggtta tttgataaaa gaattcactt atgaaaaaaa
      181 taaaaaaagc atccaaagtt acttattgaa agaaaaagaa aactatcctg ttcaacatgc
      241 tacgaaaaaa aggtggacaa ctgtccgtta tggtaatagc gatcactata tccattacga
      301 tgaaatcctt tatctaatga gcatgccagg ttataaaaat aaaataggcc tagtattgaa
      361 agatgaagag attctcgtaa acggcacaat taaaaaatat aaaaaatcac tcgaatatga
      421 gtatttatgt aaagatttaa aagcttatat cattaatact gaaaacatta catctattag
      481 cagagtaaac gaaatagtaa catttgcaaa tggagaacaa ctagagatgg gaaagagaag
      541 tattgataaa ctaaagaatt ttatgcaaga aaagatagaa aatgaatcat tttaaagaaa
      601 acaaagactc atatcttacc tgttagaaga gtgttttcct gtagaagaga acaacccaag
      661 tagcagccta gtcgtttcta tccttttttg ttatgcactc tttttgacat ttactccacc
      721 aataacgaag ggtacaataa aaagtaataa caaacaaaag gagtacagaa atgattgaat
      781 tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt aaaagacttg tctctctcga
      841 taagacaagg ggaaattttt ggatttttag gacataatgg tgccggaaaa tcaactacga
      901 ttaaaagttt agttagtatt attgaaccat catcgggaac gattttagta gatggaatga
      961 aattgacgga aaatcgattg agtatcaaac aaaaaatcgg ttatgtgcct gattccccag
     1021 atattttttt acaattgacg gctggagaat attgggactt gatttcagct gcctatgaat
     1081 tagacacaca aaaaaaagaa aagcgtttag cagaattgac agcgcttttt gatatgtata
     1141 gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat gcgtcaaaaa acgattttga
     1201 tcggcacgtt gctgccagat ccagatattt gggtattgga tgaacctttg caaggacttg
     1261 atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc tcatgccgca aaaggaaaaa
     1321 cagtcatttt ttctacacat gtattggata ccgcacaaca gttatgcgat aaactggcta
     1381 ttttgaagaa aggtgaattg atttatcagg gggcagttag tgaattattg aatggctcac
     1441 ctgatgaaac gctagaaaag atttatttga aaatggctgg aaggcaagcg acaggagatg
     1501 gaccgtatgt ataagcgaca attattagaa ttaattcgag ttaatctttt atatgttaat
     1561 ccacagctca cgaataaagc aagagagaaa ggaaaaaagg gaaagcgatt aactatctat
     1621 cttttgtctc aaaatctttt ttcagggctg atttttttac tgatttacgg atttgctatg
     1681 tttgctatga actttgttaa attgccaggt ttctttactt attacgtggc actttttggt
     1741 atgttaggtt taactcaaag tatatcagtc atttataata tcttttttga aggaaatgac
     1801 ctgcaagtat ttttgcctct gccttttgga caagaacagg ttttttcagc aaagattttg
     1861 gtagttgctt tgaccatcat tccttttgtt tttccgcttc tagtcatgtt tattttaacc
     1921 ggatggcgag ctgatctagg gattttgctt actatcatta tctcaatcct tttgttttct
     1981 ctatttttga ttattctttt ttgtctggct agtttgattg tttttggttt ggctcgaaca
     2041 gcagtgttta aaaaacatag aaaaacagtt tctagtatct tgcttggcag ctcgatgctg
     2101 atcgcatttg ctggaatcat gtggatgaat catcaaacgg ggactattga aaatgagtta
     2161 acagatcggc atccgatttc gatattattg ccattctatg atgctgccag tcagccactt
     2221 tctcaacaag gtttattggg ttggggaata ctagctatca cggctctggg atttttcctt
     2281 gctgtaaaac agctgatagt accaaagctt acagagcaac tgactgattc aaacagtgag
     2341 ttcaaaataa aaagaaccca taaaaagaac caaaatgtgc atcagctttt atttagttac
     2401 aatgctcaat tagtaaaaga acctaatttg attatgcaag ttctttcttc ttccttgtta
     2461 actcc
//
LOCUS       DARDHD010000236         2436 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000236 DARDHD010000000
VERSION     DARDHD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.239"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SDA06_002652"
     CDS             complement(<1..272)
                     /locus_tag="SDA06_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229892.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SDA06_002653"
     CDS             complement(309..446)
                     /locus_tag="SDA06_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4229893.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SDA06_002654"
     CDS             complement(448..894)
                     /locus_tag="SDA06_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229894.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SDA06_002655"
     CDS             complement(891..1040)
                     /locus_tag="SDA06_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229895.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="SDA06_002656"
     CDS             complement(1054..>2436)
                     /locus_tag="SDA06_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229896.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DARDHD010000237         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000237 DARDHD010000000
VERSION     DARDHD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.240"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SDA06_002657"
     CDS             5..1150
                     /locus_tag="SDA06_002657"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG4229897.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SDA06_002658"
     CDS             1321..1779
                     /locus_tag="SDA06_002658"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG4229898.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SDA06_002659"
     CDS             1938..>2407
                     /locus_tag="SDA06_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG4229899.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARDHD010000238         2398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000238 DARDHD010000000
VERSION     DARDHD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.241"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SDA06_002660"
     CDS             207..2069
                     /locus_tag="SDA06_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229900.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="SDA06_002661"
     CDS             2069..>2398
                     /locus_tag="SDA06_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4229901.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DARDHD010000239         2390 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000239 DARDHD010000000
VERSION     DARDHD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.242"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1983
                     /gene="esp"
                     /locus_tag="SDA06_002662"
     CDS             <1..1983
                     /gene="esp"
                     /locus_tag="SDA06_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG4229902.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEG
                     VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALP
                     EGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTV
                     EGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGT
                     KDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEK
                     VDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAP
                     NKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKV
                     IVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVF
                     PKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            2117..>2390
                     /locus_tag="SDA06_002663"
     CDS             2117..>2390
                     /locus_tag="SDA06_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4229903.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKIS"
BASE COUNT          956 a          357 c          511 g          566 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag gcgaaaaagt agaagttgga
      361 ggcacagtag atctaacaga taatgtaacc aacctaccaa cattaccaga aggaacaaca
      421 gtaacagatg tcactcctgg tggtacgatt gataccaaca caccaggtaa ttacgaaggg
      481 gttatcgaag taacgtatcc agatggtacg aaagatacag tgaaagttcc agtagaagtg
      541 acagacaatc gttccgatgc cgataaatat gaaccaacag tagaaggcga aaaagtagaa
      601 gttgggggca cagtagatct aacagataat gttactaact tgccagcgtt accagaagga
      661 acaacagtaa cagatgttac tcccggcggt acaattgata ccaacacacc aggtaattac
      721 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta
      781 gaagtgacag acaatcgttc tgacgccgat aaatatgaac caacagtaga aggcgaaaaa
      841 gtagaaattg gaggcacagt agatttaaca gataatgtta ctaacttgcc agcgttacca
      901 gaaggaacaa cagtaacaga tgttactccc ggcggtacaa ttgataccaa cacaccaggc
      961 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
     1021 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
     1081 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
     1141 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
     1201 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
     1261 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
     1321 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
     1381 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
     1441 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
     1501 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
     1561 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
     1621 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
     1681 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
     1741 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
     1801 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
     1861 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
     1921 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1981 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     2041 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     2101 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     2161 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     2221 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     2281 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     2341 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt
//
LOCUS       DARDHD010000240         2361 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000240 DARDHD010000000
VERSION     DARDHD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.243"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SDA06_002664"
     CDS             <1..1076
                     /locus_tag="SDA06_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4229904.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(1236..2141)
                     /gene="xerC"
                     /locus_tag="SDA06_002665"
     CDS             complement(1236..2141)
                     /gene="xerC"
                     /locus_tag="SDA06_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG4229905.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          765 a          471 c          406 g          719 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttgactta
     1201 tcaccattag actaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
     1261 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
     1321 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
     1381 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
     1441 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
     1501 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
     1561 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
     1621 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
     1681 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
     1741 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
     1801 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
     1861 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
     1921 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
     1981 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
     2041 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
     2101 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
     2161 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
     2221 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
     2281 aagaaaaacg caagaaaaag ggctctataa tttatgggac tgatgaatca gaattgattc
     2341 ttcagcccca ttttcatttt a
//
LOCUS       DARDHD010000241         2345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000241 DARDHD010000000
VERSION     DARDHD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.244"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..708
                     /locus_tag="SDA06_002666"
     CDS             <1..708
                     /locus_tag="SDA06_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229906.1"
                     /translation="TFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRL
                     DVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSK
                     EKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVK
                     QGFTLERASKDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVT
                     RDDGRRVRKRKDPIVLN"
     gene            complement(784..1119)
                     /locus_tag="SDA06_002667"
     CDS             complement(784..1119)
                     /locus_tag="SDA06_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229907.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1246..1461
                     /locus_tag="SDA06_002668"
     CDS             1246..1461
                     /locus_tag="SDA06_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229908.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1498..1860
                     /locus_tag="SDA06_002669"
     CDS             1498..1860
                     /locus_tag="SDA06_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG4229909.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1906..2232
                     /locus_tag="SDA06_002670"
     CDS             1906..2232
                     /locus_tag="SDA06_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229910.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          835 a          321 c          474 g          715 t
ORIGIN      
        1 acttttatag gattgataaa aaaattagat gatgaagatg ttcctttgaa gatgctcgaa
       61 tacctaaata ggttaaagga agagaaagcg ttaggaaact ttaatcataa gagaagcaat
      121 gtctatcgat tagacgtttt taatgcaact tctgtgattc aaaaattgaa tgaagatatc
      181 cttccacttc tggaaaaaat tcattttaca tatcaatatt tttcttatga atgggttaag
      241 atatgtttta acgaaaaaga ggctaaaaga gtatttcctc aaaattcccg atcagaactc
      301 agcaaggaaa aacaattgat tttagatgaa gcacatgaag ttttacaggg cttatcgtgg
      361 caagggacat atctaatgac agagaaagaa cttattaaca ctgtttgtga acgcctacgt
      421 aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa acagaggcta ctttgttaaa
      481 caaggattca ctcttgaacg agcttctaag gatattaaga aactatacaa tgtgaaagca
      541 agtagtagtt ttttaatata tttagcgaaa ggggcaggga tagatgaaac aataaaagaa
      601 ttgatattat caggagctgg gatagttcct ctgtatcaat ttacaagtgt aacaagagat
      661 gatggacgga gagtaaggaa gagaaaagac ccaattgtat taaactagac aggttatcca
      721 tagctcttaa tcttatttca tctcaataca cccaccgtta aagttcggtg ggttatttat
      781 ttttcaatct ttcaatagct gattgcgttc attttcttgc ctttttaata gcttcatata
      841 gtcaattgtt cgaactgcat cttcagtgct aagagaacga tagagagcca aaaactcttc
      901 ctcataatct acgacaacat tgtctagaaa atggcttaca ggggttccta gggctctaga
      961 aagagctgtt aagctatcga ggttaggctc tgttacagaa ttttcccatc ttgctactgc
     1021 ctgttgtgta gagaagattt tttcagctaa ttctttttgt gtccacccga gagcttttcg
     1081 cttttctcta atgacctttg catattgttt ctgtttcatt aatacacccc catctcagta
     1141 tagtacaaca agttgttgta tttgtaatgg tatttgaagt tttttatatt ttatgagttg
     1201 aaatacatca tattgttgta tataataaaa caggagggga gaatcatgaa aaaagtaagt
     1261 aatctcagcc gatacagaaa agaaaaagga ctttctcaaa cggagttagc aaaaaaaatg
     1321 aatgttacac agcaatgtgt aagttcgtgg caaacaggga gaacgattcc taaaccctat
     1381 caaatgaaga tgctttctga gatattaagt gtaccaatga atgagctgtt ttctgatgta
     1441 tttaataaag taaacagtta actaataagc aattgaatga ttttaggagg agaagacatg
     1501 tattgtagaa aatgtggcaa agaaataaca gatgaaagta agtattgcca gttttgtgga
     1561 gcactacaag aaacagatga gacaaccgga acagacacag taaagaaaag tactgagcag
     1621 acaattaatg aaatgaagga acaaatcgta catggacaag agacagggaa gctgtatttg
     1681 attattggtt ggatttctat ggtagtatcg ttagcattta ttcctgttgt atttggtgct
     1741 gttgctgtta ttatgggcta cttatatcga gaaaaagatg agaaaatggg tacgattttg
     1801 atgatagcag gggtagctgg agcaatcttt ggtgtacttt tagggatgtc aatgtactga
     1861 taagagggat aagagcaaat tattttggag ataggagata aaaagttgaa taaagtaaat
     1921 atgaacaaga agtttgtcat tataggtatt gttgtcttgg ctattatagg tattattttt
     1981 tcaaaaatga caggtacatc tggattagag aagtcatcaa aaccattggt aacacagatt
     2041 ttacaagaac agtacaactt aaatcgatca tgtgatgatg tgacgattac tcatgaaaat
     2101 ggagataaca cataccgggc taaagctata ttagacaatg gaagtgctat caacataaat
     2161 attgagtact atccaaagaa agaccgtata tatgtggaga taccatatgc tgaggtgtta
     2221 atgttaaatt gatttaaatg tattatgcat accgtccaaa caggaactcc caaaggtgta
     2281 aattcactag ggagttttta tagtttccta taaattaaat actaatacag gtgacaaaaa
     2341 aatat
//
LOCUS       DARDHD010000242         2308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000242 DARDHD010000000
VERSION     DARDHD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.245"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SDA06_002671"
     CDS             202..1077
                     /locus_tag="SDA06_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229911.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SDA06_002672"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SDA06_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..>2308)
                     /locus_tag="SDA06_002673"
     CDS             complement(1642..>2308)
                     /locus_tag="SDA06_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4229912.1"
                     /translation="NKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISG
                     SLLDPFLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRR
                     KDYKDFAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLI
                     NHVVVQIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSE
                     NET"
BASE COUNT          821 a          411 c          363 g          713 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttattt
//
LOCUS       DARDHD010000243         2305 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000243 DARDHD010000000
VERSION     DARDHD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.246"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..2154
                     /locus_tag="SDA06_002674"
     CDS             1..2154
                     /locus_tag="SDA06_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG4229913.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          789 a          413 c          512 g          591 t
ORIGIN      
        1 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
       61 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      121 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      181 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      241 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      301 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      361 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      421 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      481 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      541 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      601 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      661 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      721 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      781 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      841 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
      901 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
      961 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1021 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1081 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1141 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1201 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1261 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1321 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1381 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1441 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1501 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1561 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1621 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1681 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1741 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1801 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1861 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     1921 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     1981 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2041 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2101 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2161 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2221 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2281 gcaaaaaagg ttgtgacaaa aattg
//
LOCUS       DARDHD010000244         2287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000244 DARDHD010000000
VERSION     DARDHD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.247"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1244)
                     /locus_tag="SDA06_002675"
     CDS             complement(<1..1244)
                     /locus_tag="SDA06_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229914.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYG"
     gene            complement(1342..1617)
                     /locus_tag="SDA06_002676"
     CDS             complement(1342..1617)
                     /locus_tag="SDA06_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4229915.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            1849..>2287
                     /locus_tag="SDA06_002677"
     CDS             1849..>2287
                     /locus_tag="SDA06_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4229916.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKT"
BASE COUNT          759 a          422 c          302 g          804 t
ORIGIN      
        1 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
       61 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
      121 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
      181 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
      241 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
      301 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
      361 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
      421 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
      481 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
      541 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
      601 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
      661 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
      721 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
      781 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
      841 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
      901 tgaactaaat tgatagataa aaccttttct acgcttaagt atctactatc atctttactc
      961 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
     1021 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
     1081 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
     1141 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
     1201 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
     1261 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
     1321 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
     1381 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
     1441 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
     1501 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
     1561 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
     1621 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
     1681 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
     1741 tcgacataca cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt
     1801 gcaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
     1861 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
     1921 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
     1981 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
     2041 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
     2101 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
     2161 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
     2221 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
     2281 aagactt
//
LOCUS       DARDHD010000245         2249 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000245 DARDHD010000000
VERSION     DARDHD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.248"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SDA06_002678"
     CDS             79..1125
                     /locus_tag="SDA06_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4229917.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SDA06_002679"
     CDS             1474..2106
                     /locus_tag="SDA06_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG4229918.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          837 a          307 c          451 g          654 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattatt
//
LOCUS       DARDHD010000246         2235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000246 DARDHD010000000
VERSION     DARDHD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.249"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..2118)
                     /gene="cadA"
                     /locus_tag="SDA06_002680"
     CDS             complement(34..2118)
                     /gene="cadA"
                     /locus_tag="SDA06_002680"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4229919.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          646 a          509 c          355 g          725 t
ORIGIN      
        1 cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt ttacatgcaa
       61 gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc
      121 tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta tcccaatagc
      181 aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc
      241 aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct
      301 tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat
      361 tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct ctaaccgctg
      421 cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat
      481 atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca caccagcctg
      541 ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata
      601 accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttaaagg
      661 aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt gtttgccatc
      721 aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg gtaaaagcgg
      781 acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact
      841 cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct ggatcgaaaa
      901 gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt gcgcaagcaa
      961 ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc cgataccgcc
     1021 aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt
     1081 caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac
     1141 agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat
     1201 gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct
     1261 gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat ttttattgat
     1321 aaatccgctt aatactgttt caccaacata tacagattta gggactgatt ctcctgtaag
     1381 tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg gtacacgttc
     1441 accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca cggtcatcaa
     1501 tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt cgcgaatcga
     1561 ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat aaaatagcat
     1621 cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag caacagacat
     1681 aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct tcttcactac
     1741 atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg gttcattaaa
     1801 atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga acagcgtatt
     1861 tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg ttactttgac
     1921 atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag ccaaatgatc
     1981 ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt ccttgactcc
     2041 attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat ctaaaccttc
     2101 taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca tctcttcata
     2161 actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac
     2221 atctgcatcg gaaaa
//
LOCUS       DARDHD010000247         2234 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000247 DARDHD010000000
VERSION     DARDHD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.250"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SDA06_002681"
     CDS             <1..544
                     /locus_tag="SDA06_002681"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4229920.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SDA06_002682"
     CDS             complement(900..1472)
                     /locus_tag="SDA06_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229921.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SDA06_002683"
     CDS             complement(1488..2093)
                     /locus_tag="SDA06_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG4229922.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          339 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ctag
//
LOCUS       DARDHD010000248         2231 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000248 DARDHD010000000
VERSION     DARDHD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.251"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(43..246)
                     /locus_tag="SDA06_002684"
     CDS             complement(43..246)
                     /locus_tag="SDA06_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229923.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(256..678)
                     /locus_tag="SDA06_002685"
     CDS             complement(256..678)
                     /locus_tag="SDA06_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229924.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            916..1530
                     /locus_tag="SDA06_002686"
     CDS             916..1530
                     /locus_tag="SDA06_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4229925.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1695..>2231)
                     /locus_tag="SDA06_002687"
     CDS             complement(1695..>2231)
                     /locus_tag="SDA06_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229926.1"
                     /translation="IITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYF
                     NIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFM
                     YRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQD
                     NKDFVENIDKEPTNTVGD"
BASE COUNT          731 a          373 c          334 g          793 t
ORIGIN      
        1 agaaaaaaag aatgtcgact agacactctt tttttaaaat cattattttt ctttttgata
       61 tgttttttgg tctctttttt ttcttttttc ttcagaatct tctagattag aaattgattt
      121 ttgaattttt tgtgtctcct gtaaagttcg ctgaacttgt aaagctgatt cttctttcgg
      181 taaattaatt ttacttagaa tatcgctatc ttgataaaaa tttggtgtgt tcacaaattc
      241 tggcataaaa aatccctact ttctagttaa ggttcctgaa taagtcgcaa tccaattgtt
      301 tccagctgat ttataacttt gtaaatacaa ttttccatag taccaataac catttctttt
      361 ttctttccaa ttccgtgatt gaggaggtct agaaggccaa ccaactttat caaaaatcat
      421 cgttcctgtc actcgtatcg ttttagaagc tgctagagca agtaatggag aaactattaa
      481 ataattttca gtaacagtta agtcgttagt tgggttacca atggctattc cgtccctatc
      541 taaaggagtt aattcttggt tattatctac tacagtagtt ggttcatttg tactatcagc
      601 aaatgtagga attgtagttc cagttacact aattccaata gttaaaatac ctaaagataa
      661 aataaactta tttttcattg tctatcacct cgaaatcaaa actaagaaaa cgttttctta
      721 ataaatctta acagataaat caagttatat actagaaaat tcataaaata tatttttaat
      781 gtcataatcg tttaaatttt cttgattgac atttatttac aaaaaatatt gccatttaaa
      841 aagaaaagtg acatatggct tcctcgtgta tgattagggt gtattttaaa tagttgcaga
      901 aagtaggtaa aagttttgag agttgcatac atacgtgtct catcgatcga tcaaaatgaa
      961 caacgccaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat cgagaaacaa
     1021 tctggcgcaa cgattactca tcgacctgtt ttccaagaag cattagatta cgtccgagat
     1081 caagatattt ttattgtgga agctattgat cgacttggaa gaaattatga cgagatcatc
     1141 gcttcagtca attacttgaa gaagaaaaat gtgaagctga tcattactag tttaccgatc
     1201 atggctgaag caattggcaa tcctctcctg gataaattca ttaaggatct gatcatccaa
     1261 attttggcca tgatcgctga acaagaacgg actgaatcga aacgtcgcca ggcacaagga
     1321 attaagatcg caaaagcaaa cggcgtctac aaagggcggc ccaaattata cagtgcagac
     1381 gcaaaagatc cccaacgtcg ccttgtttac aaaagcatcg ttgaagatct taaaaatggt
     1441 gttgcgattg ctaagattgc taaagactac aatgttacga gacaaaccgt gtatagaata
     1501 aaaaaagaca gcatggtcaa tgacaaatag ttgatcatgc tgcttttatt atcttctcat
     1561 ctttaatttc ctacatataa ttactatcta taaaattaag tcttactcag aacagataaa
     1621 gcataaaatt ccaacaaacc attttttact taactaaata aagagtctgt taatataatc
     1681 ttcacttatt tacgttaatc ccctactgta tttgttggtt ccttatctat attttctaca
     1741 aaatccttat tatcttgtga tttagcttcg tataattctt tgtatgtcga cacattggtt
     1801 atactaattc ctatagctag ttcttgagac tctacttttc tctctagctc ctttgacaaa
     1861 gtgatttgag atttatttgg ttccgttgga gtcaattcta tagattcttc ataaaaatct
     1921 ctatacataa agctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     1981 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     2041 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     2101 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     2161 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     2221 gctgtaatta t
//
LOCUS       DARDHD010000249         2219 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000249 DARDHD010000000
VERSION     DARDHD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.252"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            41..559
                     /locus_tag="SDA06_002688"
     CDS             41..559
                     /locus_tag="SDA06_002688"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4229927.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            687..1370
                     /locus_tag="SDA06_002689"
     CDS             687..1370
                     /locus_tag="SDA06_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG4229928.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1679..1752
                     /locus_tag="SDA06_002690"
     tRNA            1679..1752
                     /locus_tag="SDA06_002690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1713..1715,aa:Arg,seq:tct)
     gene            1911..>2219
                     /locus_tag="SDA06_002691"
     CDS             1911..>2219
                     /locus_tag="SDA06_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG4229929.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     L"
BASE COUNT          748 a          340 c          450 g          681 t
ORIGIN      
        1 atgattaaga aggattgaat aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat
       61 aacttttata agttctataa cgttgattat atttttaatt cttattcttc tcgtcgtctt
      121 ggttccccga atttttggtt tgactcctta tattattgag gacaaagaaa tgagtccata
      181 ttatgcacaa ggaaatctga tatatgtacg accgaaaaat acaaatgaga ttttagtcgg
      241 agatattatt acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc
      301 cgttgaaaac aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc
      361 tggagtagta aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg
      421 actagtcctt tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt
      481 ttgcctaaca ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg
      541 aacacagaaa tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact
      601 cttctgaaca tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg
      661 tataatgtgt gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca
      721 aagaagaact attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca
      781 aactggaaga agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat
      841 ttttgaaatt tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta
      901 tcggtttttc atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa
      961 ttgatcgttt cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga
     1021 cagataaagt gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc
     1081 cgtatgactt tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat
     1141 gcttacgttt gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa
     1201 cgatcttgct cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa
     1261 atgaatttct tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag
     1321 gtgatggtgt gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta
     1381 tggcagcctg tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt
     1441 ctatatttgt aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag
     1501 tcaatgtagc tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt
     1561 ttaaaagatt gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta
     1621 tttcgtattg acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt
     1681 catagtagct cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc
     1741 cctcctgtga cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt
     1801 ttgtgccaat attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta
     1861 caattataag gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac
     1921 tatctagaat tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa
     1981 cggtgagaac tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa
     2041 gcagaaaatc tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag
     2101 tgggactact ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat
     2161 taatatctat tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggatttta
//
LOCUS       DARDHD010000250         2147 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000250 DARDHD010000000
VERSION     DARDHD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.253"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SDA06_002692"
     CDS             complement(<1..914)
                     /locus_tag="SDA06_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG4229930.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SDA06_002693"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SDA06_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG4229931.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          727 a          380 c          414 g          626 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactata
//
LOCUS       DARDHD010000251         2104 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000251 DARDHD010000000
VERSION     DARDHD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.254"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1539
                     /locus_tag="SDA06_002694"
     CDS             169..1539
                     /locus_tag="SDA06_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG4229932.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="SDA06_002695"
     CDS             1560..1844
                     /locus_tag="SDA06_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229933.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DARDHD010000252         2068 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000252 DARDHD010000000
VERSION     DARDHD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.255"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(388..1854)
                     /locus_tag="SDA06_002696"
     CDS             complement(388..1854)
                     /locus_tag="SDA06_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4229934.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          652 a          412 c          342 g          662 t
ORIGIN      
        1 gaataaatgg tgttcaatca gctgaccttc agcattagct caaaaagcgg caaaatatga
       61 ggagctattt ttccgtttgt ttctctaata ctcaggtcaa atcgctgatt ttacaacctc
      121 ttccttaaac ggtgttccaa agcttgcatc tgcggtaata agcccagcca aacgaaaaat
      181 atgaggggct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
      241 cctctgattc acggtgttcc agaagtagct tcttcgaaaa acaagccgaa acttcacaaa
      301 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      361 caacctcttg taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat
      421 ttttttgttt ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac
      481 gattggtgtg atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata
      541 atctactttt ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc
      601 accaaagata aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt
      661 ataagcttta ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac
      721 aactgagttc aagttcgtca accattttac taattcattc atatctttga taccaaaaat
      781 cggcaacaaa ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt
      841 acctttacga ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa
      901 caagattttt agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt
      961 tgtcaatgcg taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg
     1021 aaaagcactg tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag
     1081 catacccatt gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat
     1141 catcccttta ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt
     1201 ttccgcccct ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa
     1261 tgttggaata tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt
     1321 gatgaacggt gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt
     1381 tccggccagt ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa
     1441 ggcaagaaat ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat
     1501 atttccattt ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc
     1561 caaataagga atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt
     1621 agatgtcgta ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc
     1681 taattgtcca acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga
     1741 ggtgacaact gatactcctt gctgagcata gttattaact acattcccca tgccccaaac
     1801 catgttaaat gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc
     1861 tctcctttat gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt
     1921 acaagacagt gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat
     1981 tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
     2041 ctattttgaa ttttctgcaa aattaaaa
//
LOCUS       DARDHD010000253         2065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000253 DARDHD010000000
VERSION     DARDHD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.256"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SDA06_002697"
     CDS             <1..1008
                     /locus_tag="SDA06_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229935.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SDA06_002698"
     CDS             1025..1174
                     /locus_tag="SDA06_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229936.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SDA06_002699"
     CDS             1171..1617
                     /locus_tag="SDA06_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229937.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SDA06_002700"
     CDS             1619..1756
                     /locus_tag="SDA06_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4229938.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SDA06_002701"
     CDS             1794..>2065
                     /locus_tag="SDA06_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229939.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARDHD010000254         2062 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000254 DARDHD010000000
VERSION     DARDHD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.257"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..338)
                     /locus_tag="SDA06_002702"
     CDS             complement(<1..338)
                     /locus_tag="SDA06_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4229940.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(405..560)
                     /locus_tag="SDA06_002703"
     CDS             complement(405..560)
                     /locus_tag="SDA06_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4229941.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1177..1398
                     /locus_tag="SDA06_002704"
     CDS             1177..1398
                     /locus_tag="SDA06_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229942.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1399..1656
                     /locus_tag="SDA06_002705"
     CDS             1399..1656
                     /locus_tag="SDA06_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229943.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1792..>2062)
                     /locus_tag="SDA06_002706"
     CDS             complement(1792..>2062)
                     /locus_tag="SDA06_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4229944.1"
                     /translation="KSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKD
                     LIDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          463 c          314 g          606 t
ORIGIN      
        1 cttttagcat attgaaattt ttctggatta tccatatgag caaaatatcg caccatacct
       61 tttgcattag cacatttttg cgggtttgga gaccgaagct tttgagttat ctcacgtatt
      121 tgatcatacg tctttttacc gctaaacatt atcaaaacat gccaatgagg ctttttcaat
      181 tcaccgtcag gattcacatc tttatcatga agtggactct caacccaagg aacatgtaac
      241 tcatctaaaa actccctcca attttcagga gccgattcag gatagactac aaacgtccaa
      301 ttccttgtgc gttcatcttt agtttttcta ctcgtcatgc tataattacc ttaattgaac
      361 aacagtctaa cagcaaaagc agtgcgccaa cactaatgct tttgctattt tttttgctca
      421 ttttcctttc cgttagcatt aacccaaatt gtaataagac cagacttact aagacccatt
      481 tgtttcgcaa ttttatcaag attatctgcc acttccttag taagcgttac aacgactctc
      541 tttttttcat tactgatcat aattcgtatc ctccaacgac tttttcttaa aaaccatagt
      601 aacaaaaaag taactaaggt gcaacagttt agatactaat tagcgaataa taggccttaa
      661 acccttggca ttactgattt tgtcaaaaaa aaacgctgaa acccttggga gagtaaggct
      721 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      781 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      841 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      901 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      961 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
     1021 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1081 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1141 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1201 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1261 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1321 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1381 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1441 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1501 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1561 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1621 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1681 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1741 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1801 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1861 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1921 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1981 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     2041 atgagcgata atatcacttt ta
//
LOCUS       DARDHD010000255         2027 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000255 DARDHD010000000
VERSION     DARDHD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.258"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            641..973
                     /locus_tag="SDA06_002707"
     CDS             641..973
                     /locus_tag="SDA06_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG4229945.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1592..1933
                     /locus_tag="SDA06_002708"
     CDS             1592..1933
                     /locus_tag="SDA06_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG4229946.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          704 a          271 c          428 g          624 t
ORIGIN      
        1 taatttcaaa tatagaaaag ggaacgtatg tttgcatact cctttttttt gagttataat
       61 aatacgcgaa gagagataat ttcattattt aaacaagcta aaaatcttga tattacttct
      121 tttcaggcaa tacgaatatt attcgcttac attttttgta taaatttaat tgtctgctat
      181 gcttgtgatg aattgatttt cgttttgcac ttttggatga taaatttgga caattgaaaa
      241 tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc agcgttcata
      301 cacggcatta agatttggcc cttaatgcgt taacctttca gtgttacggt attacccgca
      361 atggatttag tataacagat attcaaaagt gataccactt tttttggctg tgttaaggtt
      421 aaaaatacgt gatattacga tgaatctatg cctaagcata atgttataaa aatgtccgtg
      481 tggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc
      541 ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat aacaagaaaa
      601 acttgttaaa aatatttaaa gaaattgggt ggtttatttt atgtcatata caggaatttt
      661 gagtctcaaa gatatttgtc actacggtaa acggtgtacc gctacagaga aaatcacgaa
      721 gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa
      781 agacaaagtc tcagaggaaa tgatttatta cattgggaaa caaaagcaaa taattttaaa
      841 agatcctatc cccttaaaag agctatatcc aacaatcaaa catgtttatg accaaaacgg
      901 tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag ggatggggcg
      961 tttgatcggc tgaattgatc attctcataa cttgtatttt atcgtttttc gcttgcttga
     1021 aaatattgtc gagacttcta aaggaactta gagtcactag aaaaataagg agtagacttg
     1081 atctatcaac aattcccttc ggggagtgac ggggtaggtt ttaacgattg gtaacgtaaa
     1141 aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat ttgtgtaaat
     1201 aataagcgca gagtaaacaa gagggaattt agaagttaga caagttagaa tagcgtctag
     1261 attaatcatg agcagctact aagggatcta gactttgacg caaactcatg gaataaggag
     1321 taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt
     1381 agatacttgc gaggacgttg attattggat caatcgagca tttaccgctg tctactaggg
     1441 tttctcgttt cggacgccat aaacgagtcg aaatttttta tgtattttta agtaaacgaa
     1501 aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa attaatataa agcatagaga
     1561 aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat tatgatccga atatacgatt
     1621 gggcacacat actattaagg tttctttcca acgttgggat tacaaaggat ttgtgacgtt
     1681 tagaaggggc ggaaattgta aaggattaga tgttttagca ttagacgagg atgatttata
     1741 cgatcaaaca ttgactgata atccaattgg attcggcttg ctacctgaag atgatgaagg
     1801 taatgaatgg tttaaaatga cgcttatgaa tgataatgga gacgaattat ctgttgaaga
     1861 tacttggtca tatttgagtg attatattgt cagctttgaa atcattgaat ttgtagcgga
     1921 taaagaagaa taaaaactgg aggaataaat tttacagata aaggcttaga aggagtcacg
     1981 acgattaata aaaaggtagt attaccttag gcattgagta aggaatt
//
LOCUS       DARDHD010000256         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000256 DARDHD010000000
VERSION     DARDHD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.259"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SDA06_002709"
     CDS             <1..378
                     /locus_tag="SDA06_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4229947.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SDA06_002710"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SDA06_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4229948.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SDA06_002711"
     CDS             1859..>2003
                     /locus_tag="SDA06_002711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229949.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARDHD010000257         1994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000257 DARDHD010000000
VERSION     DARDHD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.260"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SDA06_002712"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SDA06_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4229950.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SDA06_002713"
     CDS             853..1041
                     /locus_tag="SDA06_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4229951.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SDA06_002714"
     CDS             1054..1611
                     /locus_tag="SDA06_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4229952.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SDA06_002715"
     CDS             1643..1885
                     /locus_tag="SDA06_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4229953.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          555 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cctt
//
LOCUS       DARDHD010000258         1990 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000258 DARDHD010000000
VERSION     DARDHD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.261"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1655
                     /locus_tag="SDA06_002716"
     CDS             <1..1655
                     /locus_tag="SDA06_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4229954.1"
                     /translation="KLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALR
                     DVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINR
                     QLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKI
                     VEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVS
                     EISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSK
                     GVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCA
                     IVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDV
                     NKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIEL
                     GEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTS
                     DTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            1652..>1990
                     /locus_tag="SDA06_002717"
     CDS             1652..>1990
                     /locus_tag="SDA06_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG4229955.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKG"
BASE COUNT          760 a          266 c          344 g          620 t
ORIGIN      
        1 ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag
       61 ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg
      121 ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa
      181 tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta
      241 tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata
      301 ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct
      361 caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa
      421 atatcgactt agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa
      481 tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac
      541 cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa
      601 aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa
      661 tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa
      721 ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg
      781 cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct
      841 tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta
      901 taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata
      961 tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag
     1021 atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc
     1081 aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt
     1141 atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt
     1201 atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact gcttataaag
     1261 aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc
     1321 atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa
     1381 ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa
     1441 tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag
     1501 ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt
     1561 tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt
     1621 acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa
     1681 tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc
     1741 atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa
     1801 acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga
     1861 aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac
     1921 aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt
     1981 atcaaaagga
//
LOCUS       DARDHD010000259         1912 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000259 DARDHD010000000
VERSION     DARDHD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.262"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SDA06_002718"
     CDS             <1..177
                     /locus_tag="SDA06_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229956.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SDA06_002719"
     CDS             190..690
                     /locus_tag="SDA06_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4229957.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>1912
                     /locus_tag="SDA06_002720"
     CDS             787..>1912
                     /locus_tag="SDA06_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG4229958.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFY"
BASE COUNT          600 a          263 c          435 g          614 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta ta
//
LOCUS       DARDHD010000260         1908 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000260 DARDHD010000000
VERSION     DARDHD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.263"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..535
                     /locus_tag="SDA06_002721"
     CDS             26..535
                     /locus_tag="SDA06_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4229959.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            628..1790
                     /locus_tag="SDA06_002722"
     CDS             join(628..904,904..1790)
                     /locus_tag="SDA06_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4229960.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          672 a          343 c          383 g          510 t
ORIGIN      
        1 ttttattgtt tagggctata ctatattggt acaaggaata gaaagaggtg tttatgtggt
       61 tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac
      121 ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat
      181 tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg
      241 attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct
      301 tataagcatt tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata
      361 tggtcgaaag gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa
      421 tgaagcgtta gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa
      481 agtatcttat acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga
      541 aaaaaatgat ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat
      601 aaatgaaaac acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa
      661 gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt
      721 cgagattatg agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact
      781 ggctcgtttc aggaaaaaga taacttgatc cctgaacaag tagaactaat tgcattacga
      841 aagaaaaata aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg
      901 ggacgaaaag acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta
      961 aagtgttaaa catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat
     1021 cagaactgga agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca
     1081 ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc
     1141 gtatgatgaa acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt
     1201 cttctacttg taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa
     1261 agtcattaga agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt
     1321 atatctgcct gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga
     1381 agaaagatgc gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg
     1441 tacaactttt tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt
     1501 tatgggcctt taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg
     1561 ttgcagaatc cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga
     1621 cattggctaa tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc
     1681 tgcacggcac gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc
     1741 gaatccttga taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat
     1801 gagcttctgc cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac
     1861 cagcagcaag cttacgcaat accttataat ttttgtcaaa aaaagtgt
//
LOCUS       DARDHD010000261         1907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000261 DARDHD010000000
VERSION     DARDHD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.264"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..613
                     /locus_tag="SDA06_002723"
     CDS             <1..613
                     /locus_tag="SDA06_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4229961.1"
                     /translation="GHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELNSWA
                     DSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFAN
                     AVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCI
                     QMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            603..1745
                     /locus_tag="SDA06_002724"
     CDS             603..1745
                     /locus_tag="SDA06_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG4229962.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1758..>1907
                     /locus_tag="SDA06_002725"
     CDS             1758..>1907
                     /locus_tag="SDA06_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4229963.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTY"
BASE COUNT          680 a          251 c          400 g          576 t
ORIGIN      
        1 cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt
       61 ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt
      121 gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg
      181 tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc
      241 tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt
      301 tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg
      361 tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg
      421 cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat
      481 ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga
      541 tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa
      601 ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg
      661 ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg
      721 aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta
      781 cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg
      841 ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag
      901 tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat
      961 tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg
     1021 gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga
     1081 tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg
     1141 ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag
     1201 ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa
     1261 gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata
     1321 tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa
     1381 gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc
     1441 cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata
     1501 attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa
     1561 gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc
     1621 atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg
     1681 gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca
     1741 agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa
     1801 gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg
     1861 aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttat
//
LOCUS       DARDHD010000262         1892 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000262 DARDHD010000000
VERSION     DARDHD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.265"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            164..1309
                     /locus_tag="SDA06_002726"
     CDS             164..1309
                     /locus_tag="SDA06_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4229964.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="SDA06_002727"
     CDS             1446..1790
                     /locus_tag="SDA06_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG4229965.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          655 a          338 c          400 g          499 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat tt
//
LOCUS       DARDHD010000263         1876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000263 DARDHD010000000
VERSION     DARDHD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.267"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(320..775)
                     /gene="tnpA"
                     /locus_tag="SDA06_002728"
     CDS             complement(320..775)
                     /gene="tnpA"
                     /locus_tag="SDA06_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG4229966.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1018..1876
                     /locus_tag="SDA06_002729"
                     /pseudo
     CDS             1018..1876
                     /locus_tag="SDA06_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          593 a          364 c          354 g          565 t
ORIGIN      
        1 atacttccaa aggtgttcga taatccagtg attttctagg aagattattt ctgcgccagc
       61 attttctaat gtttgaattt gtgttttgta atcttggttg cttgtgctaa ttcttgaata
      121 gccgtaaatt gccaaaattt catcaccctt tctggacata agtttctgac accagttaaa
      181 atcaagtcta tcagtaaccc atacacccgt gaggacgggt gtatttttaa tccccctaga
      241 aggggtcgac actcgctgcc ccttaaaagg ggatttttaa aagaccgcca actgctcatc
      301 ttcactcact tacttctctt tattttttaa atggttctac gtactctctt ttacttataa
      361 tatatcgaat gcgatcatca gcttcttgtt cacggatgta tttttcattt gtcttttgat
      421 tcaatccgac tgtactcaca tagtatctct tcgaccaaaa ggatttgttt ccgtatttat
      481 atttgagatt tgcgtgctgc tcgtggatta gtaccgcact acgacctttc agatacccca
      541 taaaactgga tatacatatt tttggtggta ctcgaacaag catgtgaata tgatctagca
      601 ttgcatgtgc ttctatgatt tccacatctt tcatttcgca caacttccta agtatttttc
      661 caagatcttt tcgtaattgc ccatagatta cttttcgttg atattttggt gtaaaaataa
      721 tatgatactt acaattccaa cgagtatatg ctaaactttt atcgtcattt gacatgaaaa
      781 aactcctttc gatctgatga gcggttggta gccactcact agtatatcga aaggagtttt
      841 tatcgctaaa gatacttctt ccacgcgtag aaacgcgcgg ttttcttgtt tcgcacgctt
      901 tacgcacaaa actgactgaa gtcattaaga aaataaaagg gtctaaaact tttaagtttt
      961 gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg
     1021 gctaagtata cttttgaact taaattaaag atcgttcatg attacttgga tggtaaaggc
     1081 ggaagtgatt atctggcaaa gaaatattcc attaaggctc catcacaagt gaagcgctgg
     1141 atcaatgcct atcaagaatt tggtgaagag ggtctagtaa gaaagcgaca aaagaaaaaa
     1201 tattctgttc aatttaagct ggatgcgata gagttatatc taacaagtga gctgtcctat
     1261 cgtgaagttg ccaacacact taacatgaac aaccctaatt tgatcgctag ttggatgcgt
     1321 caattccgtg agggtgggat cgatggactc tcaaaaacga aaggatgtcc acctatcttg
     1381 tctaagaaaa atgaacccaa aaataagaaa aaatctacaa ctaaagcaac gtctaaagaa
     1441 cgtgaacgca tcgaggagtt ggagaaacga gtccgttccc ttcaaattga aaatgccttt
     1501 ttaaaagaat tgaggaagct gcgaaaacag gaagcccaac aacgacgaat gaagcaatcg
     1561 cacgaatcat cgcaagcctc cgaggaccgt ttaaattagt tgagcttcta gaaactttaa
     1621 agttccctaa ggcaatcttt atgtactggc aaaaacgatt ggaccgtaaa aatccagatc
     1681 aagagattga agcggaaatg cttaaagttc gggagaagca caaggattat ggctgtttac
     1741 ggatgaccaa tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa gttcagcgcc
     1801 tgatcaagaa gctaggaata aaagtaacga catacacacg taaatcacgg cgctacaatt
     1861 cttatcgagg acaagt
//
LOCUS       DARDHD010000264         1820 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000264 DARDHD010000000
VERSION     DARDHD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.268"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(584..874)
                     /locus_tag="SDA06_002730"
     CDS             complement(584..874)
                     /locus_tag="SDA06_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229967.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1082..1774)
                     /locus_tag="SDA06_002731"
     CDS             complement(1082..1774)
                     /locus_tag="SDA06_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4229968.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          483 a          353 c          287 g          697 t
ORIGIN      
        1 tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
       61 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
      121 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
      181 gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat
      241 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
      301 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
      361 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
      421 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
      481 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc
      541 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
      601 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
      661 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
      721 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
      781 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
      841 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
      901 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
      961 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     1021 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     1081 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     1141 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     1201 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     1261 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     1321 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     1381 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     1441 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     1501 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     1561 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag cgcttgctct ttatctgtca
     1621 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     1681 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     1741 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     1801 ttacatgcta aaagtaagtg
//
LOCUS       DARDHD010000265         1810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000265 DARDHD010000000
VERSION     DARDHD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.269"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            341..697
                     /locus_tag="SDA06_002732"
     CDS             341..697
                     /locus_tag="SDA06_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4229969.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            707..1606
                     /locus_tag="SDA06_002733"
     CDS             707..1606
                     /locus_tag="SDA06_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG4229970.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          608 a          303 c          365 g          534 t
ORIGIN      
        1 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
       61 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
      121 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
      181 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
      241 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
      301 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
      361 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
      421 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
      481 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
      541 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
      601 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
      661 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
      721 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
      781 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
      841 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
      901 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
      961 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
     1021 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
     1081 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
     1141 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
     1201 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
     1261 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
     1321 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
     1381 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
     1441 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
     1501 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
     1561 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
     1621 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
     1681 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
     1741 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
     1801 aaaaaatata
//
LOCUS       DARDHD010000266         1792 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000266 DARDHD010000000
VERSION     DARDHD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.270"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..258
                     /locus_tag="SDA06_002734"
     CDS             <1..258
                     /locus_tag="SDA06_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG4229971.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            356..565
                     /locus_tag="SDA06_002735"
     CDS             356..565
                     /locus_tag="SDA06_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG4229972.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            582..854
                     /locus_tag="SDA06_002736"
     CDS             582..854
                     /locus_tag="SDA06_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4229973.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            856..1719
                     /locus_tag="SDA06_002737"
     CDS             856..1719
                     /locus_tag="SDA06_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG4229974.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          726 a          282 c          322 g          462 t
ORIGIN      
        1 atgatcggtt ttgttcctta tttagttgat acggacagcg caacgataaa atcaaacctg
       61 gaagaactgt ataagcaaca taaagaagat aacttggttt tccaaaatat tatcaagcga
      121 agtaataaag taagtacctg gtctaaaaac ggcattacag agcataaagg ctatgacaaa
      181 aaagttttat ccatgtataa gaacgtattt tttgaaatgc ttgagcgaat tattcaatta
      241 gaaaacgaaa aagaatagaa tcacaaatca caagtgatta atcacaaatc acttgtgatt
      301 tgtgattgtt gatgataaaa taagagtaag aagaaataga aagaagtgag tgattgtggg
      361 aaatttaggc gcacaaaaag caaaacgaaa tgatacacca atcagtgcaa aaaaagatat
      421 aatgggagat aagacggttc gtgttcgtgc tgacttgcac catatcataa aaatcgaaac
      481 agcaaagaat ggcggaaacg taaaagaagt tatggatcaa gccttagaag aatatatacg
      541 gaaatattta cctgacaaac tttaaaagga gtgaaaatga aatggcagtt acgtatgaaa
      601 aaacatttga aatagagatc attaacgaat tatcggcaag cgtttataat cgagtattaa
      661 actatgtttt gaaccatgaa ttaaataaaa atgactctca attattggaa gtcaatttat
      721 taaaccaatt aaagcttgca aaacgtgtaa atctttttga ttattcttta gaagaattac
      781 aagccgttca tgagtattgg cggtcaatga atcgttactc aaaacaagtt ttgaataaag
      841 agaaagtggc ttaatatggc aaatatagtc aattttactg acaaacaatt tgagaatcgc
      901 ttaaatgata atttagaaga attgattcaa ggaaaaaaag cggttgaatc gccaaccgct
      961 tttttacttg gtgggcaacc agggtcaggg aaaaccagtt tgcgatcagc aatttttgaa
     1021 gaaacacaag ggaatgttat tgtcattgat aatgatacct ttaaacaaca gcaccctaat
     1081 tttgatgaac tagtgaaact ttatgaaaaa gacgtagtaa aacacgttac cccttattct
     1141 aatcgcatga cagaagcgat cataagccgt ttgagcgatc aagggtataa tttggtgatc
     1201 gaaggtacag gacgaacaac agacgttcct attcaaaccg caacaatgct tcaagccaaa
     1261 ggttatgaaa caaaaatgta tgtcatggca gtacctaaaa tcaactcata tttaggaaca
     1321 attgaacgat atgaaaccat gtatgcagat gatccaatga cagccagggc aacaccaaaa
     1381 caagcgcatg atattgttgt caaaaactta ccgaccaatt tagaaaccct tcataaaacg
     1441 ggcttattta gcgatataag gctatataac agagaaggag taaaactcta ttcaagtttg
     1501 gaaacacctt ctattagtcc aaaagaaacc ttagaaaaag aattgaatcg taaagtatca
     1561 gggaaagaaa ttcaaccgac tttagaacga atagagcaaa aaatggttct aaataaacac
     1621 caagagacac ctgaatttaa agcaattcaa caaaaattgg aaagcttgca gccacctaca
     1681 ccaccaatac ccaaaacacc taaacttcca ggtatttaaa ccttaaaaaa ggaaaagagt
     1741 agttaccaaa aaacggtaac tactcttttt ttataaaaac atttcctcat gt
//
LOCUS       DARDHD010000267         1748 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000267 DARDHD010000000
VERSION     DARDHD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.271"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SDA06_002738"
     CDS             complement(<1..182)
                     /locus_tag="SDA06_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4229975.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SDA06_002739"
     CDS             517..951
                     /locus_tag="SDA06_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4229976.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SDA06_002740"
     CDS             974..1294
                     /locus_tag="SDA06_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229977.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SDA06_002741"
     CDS             1435..1626
                     /locus_tag="SDA06_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229978.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          666 a          261 c          364 g          457 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattact
//
LOCUS       DARDHD010000268         1727 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000268 DARDHD010000000
VERSION     DARDHD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.272"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="SDA06_002742"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="SDA06_002742"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4229979.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          526 a          356 c          367 g          478 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
     1681 tttattcact acctttattt tcctattgaa ttagcttttt atctaat
//
LOCUS       DARDHD010000269         1724 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000269 DARDHD010000000
VERSION     DARDHD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.273"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..521
                     /locus_tag="SDA06_002743"
                     /pseudo
     CDS             1..521
                     /locus_tag="SDA06_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            676..1251
                     /locus_tag="SDA06_002744"
     CDS             676..1251
                     /locus_tag="SDA06_002744"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4229980.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1294..1491)
                     /locus_tag="SDA06_002745"
     CDS             complement(1294..1491)
                     /locus_tag="SDA06_002745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229981.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1478..1660
                     /locus_tag="SDA06_002746"
                     /pseudo
     CDS             1478..1660
                     /locus_tag="SDA06_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          603 a          273 c          342 g          506 t
ORIGIN      
        1 gattttacta cagaaatcct aaagactcta gcgaacaaag gcgatttgaa tggattattc
       61 cgtgtccatt tggaaaaagc tgtcaatacg cttctcaaaa cggagttaac ggctttcctc
      121 gattacgaaa agtacgatcg cattggtttt aacacgggta attctcgtaa cggctcctat
      181 gaccgtacgg tcaagaccga gtatggggaa cttcatctcc agattccgcg cgaccgcaat
      241 ggcgagttca agcaacagac tgttcctgct tatagacgga cgaatgacac attagaggag
      301 accgtcattc acctcttccg aaaaggtatt accatgtcgg aaatcgcaga cttgattgag
      361 aaaatgtatg ggcatcacta cacgccccaa accatgtcca acatgactaa agtgctgact
      421 gaagaagtaa atgcatttaa agttagaact ctaaatgata agtatgtcgc tattttcatg
      481 attccttcag tttttcagat gatataccac tcaaaaaaca atatgagaga tgttcaggaa
      541 taccatctct aatggcttat aaattggttt tagggatagt agatgttata gacatatcaa
      601 ggaataaacg agcagctgca taaaagcaga tggaattaag gaataattga attgtaaaag
      661 aagggaataa atttaatggc acaattattt tacagatatg gaacaatgaa tagtggaaag
      721 agtattgagc tactgaaagt ggctcataat tatgaagaac aagggaagag agttatcata
      781 atgacaagcg caatagatac tcgatcagga tttggaaaag ttgagagtcg tataggtgtt
      841 tcgaaaccag ctattgcaat tacttctgaa atggatgtgt acggagaatt agtggagatt
      901 attgatcagt acggaaaagt tgattgtatt ttggtcgatg agtctcaatt tttaagtaga
      961 gagaatatta ttgatctatc acttatcgta atgaatgagg aagtaccagt aatttgctat
     1021 ggattgaaga atgattttca aaataatctc ttcaagggat ccgaagcatt attgacattt
     1081 gctgataaaa ttgaggaaat taaaactatt tgccattttt gcaataaaaa ggctgtaatg
     1141 aatgctcgta ttgttgatgg tattccagtt tattcaggtg aacagattgt agtaggaggt
     1201 aatgaagatt acctttcggt atgtcgcaac cactactaca acgaagattg aaataaaaaa
     1261 cgtgtaatca aataattgat tacacgtttt ttattattct gttaaaggtt gaggatcaga
     1321 aatttttagg tatctataat cctcacctaa aaatccgtta aaacgaagta aaacatcatg
     1381 accatttaaa tcagttggaa ctaaaattgg aattaattta tcaacagtag ttttgttgta
     1441 aagttcaaaa gtgttctcca ttccttctgg ttctacaatc gaatctttca ataaacaaat
     1501 caagaaatac agccgcagaa aagagcagtt tcaaaatgaa gaatcactag agcgtttcct
     1561 agtatccatc tttgatacat acaatcaaaa attcttaaat agaagccata aaggcttcca
     1621 acaagtgacg gatacattag cttcaatgtt tactgagtaa cccattattt tgcagaagga
     1681 cgagttattt acacaaaatt attgacgctc cctgtttgga gtgg
//
LOCUS       DARDHD010000270         1721 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000270 DARDHD010000000
VERSION     DARDHD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.274"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..906
                     /locus_tag="SDA06_002747"
     CDS             <1..906
                     /locus_tag="SDA06_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4229982.1"
                     /translation="NLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFV
                     KNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDI
                     NFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQ
                     FFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLS
                     KTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG
                     GIKKDIKLRVEDSTLIEQIFNDITK"
     gene            924..1493
                     /locus_tag="SDA06_002748"
     CDS             924..1493
                     /locus_tag="SDA06_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG4229983.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1603..>1721
                     /locus_tag="SDA06_002749"
     CDS             1603..>1721
                     /locus_tag="SDA06_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229984.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSI"
BASE COUNT          612 a          207 c          323 g          579 t
ORIGIN      
        1 aatttggact tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa
       61 ggatttacta ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa
      121 gttctatttg ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa
      181 aacaataacg cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc
      241 gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa
      301 gacataaatt ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg
      361 gggaaaacaa ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata
      421 atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa
      481 tttttcacag aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat
      541 cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt
      601 gatttatccg gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact
      661 aatttatttc tatttgatga acctacaagt gggttggatt atattaatat ggaaaggatt
      721 gctgacctta ttaaagactt aaaagaaaag ggtgcagtag ttatagctac tcatgatccg
      781 gaatttcttt ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa
      841 gatataaaac ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg
      901 aaatagaatt gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta
      961 cagttatttc cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt
     1021 ttggtcacag catatccccc gggatttttg gattattatc tggaatcata tttgtttata
     1081 ttagctataa ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat
     1141 tttttacgtt aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga
     1201 tcttagctga tattattcta tctgtatttg gatatgatcg agcaattcct caagttgcgt
     1261 cttgggctct aatgcaatta ggcagtgctg ccggacagtg gattcctata tggttcttta
     1321 cagatcgttt tcgtcaagat tggatagata aaggacaatc tgctgcaaca atggatgcaa
     1381 tgatccacta tgcggtaggt atatggggaa tcatttcagt gttagttgta gcaagtttat
     1441 ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag
     1501 tttacctttt atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt
     1561 gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa
     1621 ttatttagtt atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat
     1681 ttttctgtcg tattaacagt ctatggatat tacagtattt a
//
LOCUS       DARDHD010000271         1714 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000271 DARDHD010000000
VERSION     DARDHD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.275"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(366..1169)
                     /locus_tag="SDA06_002750"
     CDS             complement(366..1169)
                     /locus_tag="SDA06_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4229985.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          539 a          327 c          211 g          637 t
ORIGIN      
        1 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
       61 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
      121 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
      181 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
      241 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
      301 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
      361 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
      421 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
      481 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
      541 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
      601 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
      661 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
      721 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
      781 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
      841 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
      901 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
      961 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     1021 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     1081 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     1141 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     1201 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     1261 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     1321 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     1381 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     1441 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     1501 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     1561 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     1621 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     1681 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DARDHD010000272         1662 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000272 DARDHD010000000
VERSION     DARDHD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.276"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1042
                     /locus_tag="SDA06_002751"
     CDS             <1..1042
                     /locus_tag="SDA06_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4229986.1"
                     /translation="LLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLF
                     LLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLI
                     FLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYK
                     NLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKL
                     YQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRD
                     DKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQND
                     PYQFTTQVILLF"
     gene            1057..>1662
                     /locus_tag="SDA06_002752"
     CDS             1057..>1662
                     /locus_tag="SDA06_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4229987.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRI"
BASE COUNT          627 a          193 c          273 g          569 t
ORIGIN      
        1 attactatta tgtatgctta ttgatgattt cagatctatt attacttcca tggttatgac
       61 gtatatgttt tcatttgaag ggataaaagg gtatggttat gtagtattcc agataatgct
      121 tgattctttg tttttgctga tgtttatttg gttatataga aaattagcta taaataggat
      181 tgttgaacag tccagttcta aacttactgc tatattcatg atttatttac ttacaattgg
      241 tttatttgtt tcttatgttg ctcatcaata tcaagtattt gatcattttg tgtttggtgt
      301 cttaatattt ttaatcattc aaatgtttgt tgttctattt ttatttatta gaataactat
      361 taggcaacga gaacaatatg aacagcaatt gaaaagaaaa gaattggttt atctaaaaaa
      421 atatactgat tcactggaaa aagatcaaga aaaatttgcc agatttagac atgactataa
      481 aaatttattg ctgagtttaa aagaaatcgc taatcaaaac catgattcga tattggaaaa
      541 agaaataaaa gaattagaga tctattctaa ttcttatcta accaataagt ttgaatatcg
      601 ttatttaaaa aatataaaaa atgaatattt gaaaagtttg ttgatagcga agctatacca
      661 agcaaataac aataagattt gttgtaaatt cgaatgtcca agtactatta ttgaagttcc
      721 tgttccaata tttgactgta ttagggtgct tggaattatt ctcgataatg caattgaagc
      781 atctatagag agtcaagata gagaactgtc tctagctatt taccgagatg ataaacagat
      841 agaaatttcg atatcgaata gttgtagaga gatactactc tctatagata ctctaatgaa
      901 aaaaggaata acaacaaaaa agggacatca agggttaggt ttatctagta tagaggaaat
      961 caataaaaag aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc aatttactac
     1021 tcaagttatt ttgttgtttt gagggaagga ggatttatga gttatccaat aatattatgt
     1081 gaagatcaaa ttatacaatt aaatcagtta gaacgaatta ttgataattt cattcttttt
     1141 cacgataaag tatttaagat agtattgaaa acacaaagtc cgttagaagt taaaaaatac
     1201 ctcaaacaat tccgaccaaa gcaaggaata tatttcttag atattgattt aaatcatgaa
     1261 gttaacggta tagaattagc agaagtaatc agaaaatatg atgttcaagc aaaaatcatt
     1321 tttacaacta ctcatgatga gatgttaccc gtaacaataa aaagaagagt tgaaacgtta
     1381 ggatttgtaa caaaagatca aacactagat gagtatcgaa acgagattgt tgagttattg
     1441 ttattagcgc aagaaaggat agatgcaaca aaacaggtta cgaatcaagc atttgttttt
     1501 tccataggtt ctcaaacatt tacttttgat ataaatgaca tttattttgt agaagcttca
     1561 aaattaccac atagattatc attgtgtact aaagatgggc agtacgaatt ttatggaaga
     1621 attagtgagc ttgaaaagaa atatcctatg ttaacaagaa tt
//
LOCUS       DARDHD010000273         1656 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000273 DARDHD010000000
VERSION     DARDHD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.277"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..464)
                     /locus_tag="SDA06_002753"
     CDS             complement(<1..464)
                     /locus_tag="SDA06_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4229988.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGC"
     gene            complement(475..1143)
                     /locus_tag="SDA06_002754"
     CDS             complement(475..1143)
                     /locus_tag="SDA06_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4229989.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1273..>1656)
                     /locus_tag="SDA06_002755"
     CDS             complement(1273..>1656)
                     /locus_tag="SDA06_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4229990.1"
                     /translation="NLENIDIEKSWRENFNELDPFINYIFENKRIFDLILFQSAGTKY
                     QDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSY
                     SKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          598 a          278 c          214 g          566 t
ORIGIN      
        1 ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat gtttttatct
       61 aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc catatgctct
      121 tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt tgttgtaaag
      181 aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt
      241 aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt
      301 cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca
      361 gtaatcaatg taagactgcc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc
      421 ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat
      481 caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat
      541 gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt
      601 agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc
      661 caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt
      721 aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa
      781 cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata
      841 gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa
      901 ggatgtcgca aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac
      961 taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat
     1021 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
     1081 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
     1141 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
     1201 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
     1261 agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct
     1321 tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat catagaatgt
     1381 agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta aattttgatt
     1441 gatcattccg atagtcttta acttatctag acaacagact gtctttttcg tcacaaatga
     1501 tgtaattttt tcaacaatat cttgatattt ggttccagct gattggaata aaatcaaatc
     1561 gaatatacgt ttattctcaa aaatataatt aataaatgga tctaattcat tgaagttctc
     1621 tctccaactt ttctctatat ctatattttc taagtt
//
LOCUS       DARDHD010000274         1649 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000274 DARDHD010000000
VERSION     DARDHD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.278"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            250..900
                     /locus_tag="SDA06_002756"
     CDS             250..900
                     /locus_tag="SDA06_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229991.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            1087..>1649
                     /locus_tag="SDA06_002757"
     CDS             1087..>1649
                     /locus_tag="SDA06_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG4229992.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          529 a          195 c          336 g          589 t
ORIGIN      
        1 aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt ggataaggat
       61 ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta atgtagaagt
      121 tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa atagtaattg
      181 ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg gtttagaatt
      241 tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac ttttactatc
      301 attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac agcattttta
      361 ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt tggatttttt
      421 ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc agagattaca
      481 tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat tttgattgaa
      541 gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac tcaacctaga
      601 cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt tcattatgaa
      661 ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc tgctggatat
      721 tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat tgcaggtttt
      781 atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga atatgggatc
      841 acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga ttattattag
      901 tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc cattttttgt
      961 ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta tcaatgaggt
     1021 tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga attggaggaa
     1081 ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc ggctttgaga
     1141 tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca ctatactttt
     1201 tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt tatgaaaatt
     1261 aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca aaggaaaaga
     1321 gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt gacttcttca
     1381 attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat acgttattta
     1441 tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag caagaagaat
     1501 aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat tatactgttt
     1561 cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct tatactgttt
     1621 tctgttttat ttggggtgat gtttataga
//
LOCUS       DARDHD010000275         1647 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000275 DARDHD010000000
VERSION     DARDHD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.279"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..326)
                     /locus_tag="SDA06_002758"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="SDA06_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            601..1143
                     /locus_tag="SDA06_002759"
     CDS             601..1143
                     /locus_tag="SDA06_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229993.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1299..>1647
                     /locus_tag="SDA06_002760"
     CDS             1299..>1647
                     /locus_tag="SDA06_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG4229994.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          601 a          218 c          314 g          514 t
ORIGIN      
        1 cgctgatatg gaatatttat gcttgttggc atcaattact tgtcttttcg tccgaatatc
       61 agcgccgctt gctttaaaat atcattctcc atttcgagtt gcttattttt ctttcttagt
      121 gcgatcaatt ctgcttgttc tggtgttaag ttgtctcttt ctttgaatga gcccgttgat
      181 tgtgcttgct tcatccattt atcgaaagaa gaaggcgtaa gctcatactc tcgaataatt
      241 tctgcgcgag gcttaccagc gagatagaga tcgacgattt gttgtttgaa ttcttttgaa
      301 taggttcttc gttgacgtct tgacatgaaa aatcctccag tgtgtttttt attattctac
      361 acaccttatt ttttctgtct agtctagtgt agccgattca aattgatgaa gcagatccca
      421 aagggctgct tcatcaattt tttcgtaagt gtccgtacac ttactgtgct tgctcatata
      481 ttaatcgatc atatactaat cgtgtaagaa caactttaag cataatactt atattgttat
      541 accatagttt ggaaaaataa aaaattatat ttcatcaacg tttcataaaa ggtgtgtaga
      601 atgttattga aagtagctat aaaatgctat tgtgtaaaaa ataaaggagg aataatttat
      661 gaaaaaatag taccaagagg agcatgccta ttacttgcat tatgtttgag cttaagcgca
      721 gttgggtgtc aaaaaagtaa tgaaaacaat aaaataaaag aaggacaatc cataagttct
      781 gaagaggaag gcatgtcaat taatgcaaat ggcaaaaatg aaacttttaa gccatcagat
      841 tatacattgg aggcaaagaa agaatatgtt tatgaatatt tagggcttaa atttaaactc
      901 tcagacaaat ttagaaatta tatagctgat aaaaagatag cgatgttaga tgatcaaagt
      961 cctatagata aagaactaaa gtatgctatt ttgacatttg agaaaatgac agaagaacaa
     1021 aagaatgcag ttatagaaaa gatgggcgat ggatataaaa attggcaaaa tgagcttgaa
     1081 agaattggga ctattggtat atttgaaaaa aatacatcag aagaaaaaaa tctaaaatta
     1141 taaaatgtga tactcatact aaaataggag tttcaagtga tggaaaatac gattgctatt
     1201 taagcacaaa tagcggagct gaaagtaatc ttctggatga attaaaaaga actgagattc
     1261 aaattataga taaaaaagaa cgtcctaaaa atggttttgt gctatcagaa aaaactgatt
     1321 tagaaaatac agaagcattt aacaaagaat cggttaaaga tttacgtaaa ctaccaacaa
     1381 aggacataaa tggcaaggat tttacaagta aagattttga gaagtatgat cttactatgg
     1441 taaatgtatt tgcaacttgg tgtacagctt gtgtaaagga aattcctgat ttggttgagg
     1501 ttcagaatga aatgaaaagc aagggtgtaa atatcgttgg agttgttaca gatcctgttg
     1561 atgataacgg tgaaaacaag gaagcaattg aaaagtcaaa gttaatacat gaaaagacaa
     1621 aggcttcata tcctttctta atgccag
//
LOCUS       DARDHD010000276         1638 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000276 DARDHD010000000
VERSION     DARDHD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.280"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1594
                     /locus_tag="SDA06_002761"
     CDS             128..1594
                     /locus_tag="SDA06_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG4229995.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          460 a          296 c          336 g          546 t
ORIGIN      
        1 attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt aaataagttt
       61 tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt
      121 atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac
      181 tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat
      241 ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt
      301 agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt
      361 atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct
      421 tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt
      481 gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta
      541 ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt
      601 aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt
      661 agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg
      721 acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg
      781 taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg
      841 ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg
      901 gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga
      961 agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc
     1021 attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac
     1081 gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt
     1141 tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac
     1201 agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt
     1261 gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt
     1321 cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa
     1381 attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc
     1441 ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta
     1501 cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga
     1561 accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc
     1621 ttatttgcag cagttcct
//
LOCUS       DARDHD010000277         1603 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000277 DARDHD010000000
VERSION     DARDHD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.281"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..133)
                     /locus_tag="SDA06_002762"
     tRNA            complement(60..133)
                     /locus_tag="SDA06_002762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Ile,seq:gat)
     gene            complement(146..216)
                     /locus_tag="SDA06_002763"
     tRNA            complement(146..216)
                     /locus_tag="SDA06_002763"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Gly,seq:tcc)
     gene            complement(221..293)
                     /locus_tag="SDA06_002764"
     tRNA            complement(221..293)
                     /locus_tag="SDA06_002764"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Phe,seq:gaa)
     gene            complement(297..369)
                     /locus_tag="SDA06_002765"
     tRNA            complement(297..369)
                     /locus_tag="SDA06_002765"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Asp,seq:gtc)
     gene            complement(388..461)
                     /locus_tag="SDA06_002766"
     tRNA            complement(388..461)
                     /locus_tag="SDA06_002766"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Met,seq:cat)
     gene            complement(474..563)
                     /locus_tag="SDA06_002767"
     tRNA            complement(474..563)
                     /locus_tag="SDA06_002767"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(525..527),aa:Ser,seq:tga)
     gene            complement(586..657)
                     /locus_tag="SDA06_002768"
     tRNA            complement(586..657)
                     /locus_tag="SDA06_002768"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(622..624),aa:Glu,seq:ttc)
     gene            complement(679..766)
                     /locus_tag="SDA06_002769"
     tRNA            complement(679..766)
                     /locus_tag="SDA06_002769"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Ser,seq:gct)
     gene            complement(791..864)
                     /locus_tag="SDA06_002770"
     tRNA            complement(791..864)
                     /locus_tag="SDA06_002770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(828..830),aa:Ile,seq:gat)
     gene            complement(878..948)
                     /locus_tag="SDA06_002771"
     tRNA            complement(878..948)
                     /locus_tag="SDA06_002771"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Gly,seq:tcc)
     gene            complement(950..1025)
                     /locus_tag="SDA06_002772"
     tRNA            complement(950..1025)
                     /locus_tag="SDA06_002772"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(990..992),aa:Asp,seq:gtc)
     gene            complement(1044..1117)
                     /locus_tag="SDA06_002773"
     tRNA            complement(1044..1117)
                     /locus_tag="SDA06_002773"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Met,seq:cat)
     gene            complement(1130..1219)
                     /locus_tag="SDA06_002774"
     tRNA            complement(1130..1219)
                     /locus_tag="SDA06_002774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1181..1183),aa:Ser,seq:tga)
     gene            complement(1243..1316)
                     /locus_tag="SDA06_002775"
     tRNA            complement(1243..1316)
                     /locus_tag="SDA06_002775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1280..1282),aa:Ile,seq:cat)
     gene            complement(1330..1403)
                     /locus_tag="SDA06_002776"
     tRNA            complement(1330..1403)
                     /locus_tag="SDA06_002776"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1367..1369),aa:Met,seq:cat)
     gene            complement(1431..1503)
                     /locus_tag="SDA06_002777"
     tRNA            complement(1431..1503)
                     /locus_tag="SDA06_002777"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1468..1470),aa:Ala,seq:tgc)
     gene            complement(1523..1596)
                     /locus_tag="SDA06_002778"
     tRNA            complement(1523..1596)
                     /locus_tag="SDA06_002778"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Pro,seq:tgg)
BASE COUNT          434 a          441 c          383 g          345 t
ORIGIN      
        1 tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccaa taaaataaat
       61 gggcctaaat ggactcgaac catcgacctc acgcttatca ggcgtgcgct ctaaccagct
      121 gagctatagg cccataaaaa ctaaaagcgg gtgacgagaa tcgaactcgc gacaacagct
      181 tggaaggctg tggttttacc actaaactac acccgcataa tggcgcggga cagaatcgaa
      241 ctgccgacac atggagcttc aatccattgc tctaccaact gagctaccga gccaaacggt
      301 tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta acccctacac
      361 cacggaacca atgtttttag tttttaattg cgggagcagg atttgaacct acgaccttcg
      421 ggttatgagc ccgacgagct accagactgc tccatcccgc gataatatga aaaaggagga
      481 taagggattc gaacccttgc acggttttac ccgcctgacg gttttcaaga ccgttccctt
      541 cagccggact tgggtaatcc tccatgtaaa actatattaa attaatgacc cgtacgggat
      601 tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca acgggccagg
      661 taaaacaaaa tattgaaacg gagaaggagg gatttgaacc ctcgcgccgg tttcccgacc
      721 tacaccctta gcaggggcgc ctcttcagcc acttgagtac ttccccaaaa ccaataagat
      781 tttgtactaa tgggcctaaa tggactcgaa ccatcgacct cacgcttatc aggcgtgcgc
      841 tctaaccagc tgagctatag gcccataaaa aactaaaagc gggtgacgag aatcgaactc
      901 gcgacaacag cttggaaggc tgtggtttta ccactaaact acacccgcat ggcggttccg
      961 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
     1021 gaaccagtgt ttttagtttt taattgcggg agcaggattt gaacctacga ccttcgggtt
     1081 atgagcccga cgagctacca gactgctcca tcccgcgata atatgaaaaa ggaggataag
     1141 ggattcgaac ccttgcacgg ttttacccgc ctgacggttt tcaagaccgt tcccttcagc
     1201 cggacttggg taatcctcca tatgaaactt acaacgccac aatggacctt gtaggactcg
     1261 aacctacgac cggacggtta tgagccgtct gctctaacca actgagctaa aggtccaggc
     1321 tcttaaaaat cgcggcggag gggatcgaac ccccgacctc ccgggtatga accggacgct
     1381 ctagccagct gagctacacc gcgaaaaaga tattcattat ttaaatgaaa tggagcctag
     1441 cgggatcgaa ccgctgacct cctgcgtgca aagcaggcgc tctcccagct gagctaaggc
     1501 cccgtgttta aatttataat tatcgggaag acaggattcg aacctgcgac cccttggtcc
     1561 caaaccaagt gctctaccaa gctgagctac ttcccgttta att
//
LOCUS       DARDHD010000278         1584 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000278 DARDHD010000000
VERSION     DARDHD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.282"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1472
                     /locus_tag="SDA06_002779"
     CDS             <1..1472
                     /locus_tag="SDA06_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229996.1"
                     /translation="IILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARVKGLSSI
                     FSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNVCTIMLM
                     FHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYYAYALIY
                     EKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRLIVELSK
                     AVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDLTTFTVG
                     IIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMIQFPWRI
                     LGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKVVTQRDH
                     LVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQVMDSKIV
                     YTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
BASE COUNT          483 a          262 c          285 g          554 t
ORIGIN      
        1 aaataatatt gtccattctc ctagcaatta ttgctggact ttattcaatc aattatttta
       61 gaaatgttca tactatttct acatcggggg atattgcctt ccattttgct agagtcaaag
      121 ggttatcttc aattttttct ggtccaatca attttacgac gtttaatcat tatgggagcg
      181 gagtgaatta tttttatccc tacttgacct tttttccggc agtcattttt tattggataa
      241 gtaataattt aatcgttagt tatatcttat atgtttggct attgaatgtt tgtactatca
      301 tgctgatgtt tcattatgga ctaaaatttt taaaaagaat cgatgcggct tttatattta
      361 gctgtttata tacattttat gggtaccgta cgattgatat ttatcatcgt tctgctattg
      421 ctgaagcgat tgcacttacg gttataccga tagttatgta ttatgcttac gcactgattt
      481 atgagaaaaa gccatgtgca attccattag cagtcagtat gagcttactc atttatacac
      541 atgttctttc cacttttatg agtgtaatag ccgttggctt actagtctta ggcggctttg
      601 tagtaagtga aacaagaaaa aaagaaagca tccggttgat tgtggagttg tcaaaggcag
      661 taggaatgac gattctttta acagcatact tttggtatcc gatgttccga caaatcatgg
      721 ttcaatcgat taatgagcca gataaaacga ccttacaaca ccaagcatta aatgtctcgg
      781 atagtttgat tggtgcgatg aataatgacc tgacaacctt tacggtagga atcattggta
      841 ttatttcttt gatctgtcca ctcttctttt ttaaaggatt taatcgcaaa gaaaaagtga
      901 tttaccttag tgcggtagtc tcttggctct tatccaccaa attttttcca tggtctttat
      961 tacaaaatac acccattcaa atgattcaat ttccttggcg aatcttaggc tttcaagtac
     1021 tgtttggctc gttgatttta tcgttcgttt tcatgaagag cacatcagct aagcaaagaa
     1081 aagtagttaa tataacggca attgttttat tactgttggc tgtatctgct gcaacaaaag
     1141 caaactatga acataaagtt gtcactcaga gagatcactt ggtaatgaat caagaaaatc
     1201 ttattcgtta cacgacactc cttcccggag gactttatga ctatgcaccc acggaagcat
     1261 tacaatacaa agaacatttg cagaatcatg aagtaagaat tggcaaggaa tggcatacta
     1321 tgccttatca agtgatggat tcgaaaatag tctatacagt acatgaatct aaaggacaaa
     1381 aaataacgct tcctgtattt gattattggg gaacagtcgc aaaagtaaat ggacaaaaaa
     1441 gagagattga taataggact agtgtcgatt gaaggaacac aaggagaaac aatcattgaa
     1501 gtttcttcca tttatcctcc aactatgaag ttagcttttt taattagttt atgtagttac
     1561 ctctttcttt taattagttt tctt
//
LOCUS       DARDHD010000279         1582 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000279 DARDHD010000000
VERSION     DARDHD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.283"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..396)
                     /locus_tag="SDA06_002780"
     CDS             complement(19..396)
                     /locus_tag="SDA06_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229997.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(696..1577)
                     /gene="galU"
                     /locus_tag="SDA06_002781"
     CDS             complement(696..1577)
                     /gene="galU"
                     /locus_tag="SDA06_002781"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4229998.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          435 a          357 c          241 g          549 t
ORIGIN      
        1 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
       61 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
      121 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
      181 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
      241 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
      301 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
      361 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
      421 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
      481 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
      541 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
      601 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
      661 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
      721 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
      781 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
      841 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
      901 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
      961 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     1021 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     1081 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     1141 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     1201 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     1261 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     1321 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     1381 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     1441 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     1501 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     1561 gcttttttga ccttcacttg ct
//
LOCUS       DARDHD010000280         1562 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000280 DARDHD010000000
VERSION     DARDHD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.284"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          589 a          179 c          316 g          478 t
ORIGIN      
        1 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaattt
       61 aatttgacaa ttaagaaagt attaacttgt tttgtgaaaa agagaatttg aaaaacgatt
      121 ggcgaacaaa tgctcggtag gtatggtata aatacagagc taattggtgt gatgaaatca
      181 gatattcgcg tgagattcag taaagtagga gttcgaaaac agctaggtga caaacagtat
      241 tgctttggta cttcggaatg gtttatcaaa ttcttatcaa cagttcactt ctctttatgt
      301 ttgagtgatg attcgttagg ttattgcttg gaaagaccgg atgagagtag aagagtttaa
      361 ttttataaag tgaaaagagg taatgtacga tatacgtagg gctgaaccaa tagaggataa
      421 gcaaacgatg ttacaggcgc aaaattcata gcttactgtt ttggagataa cagaagagga
      481 aataatgcag aagtaaggcg aaaaaaggac ggaagcctaa cagcatatga aataaagaaa
      541 aatattataa ctactgataa atcggtattg agcaaagtac taataggagt caaaaacatt
      601 cataagctat gagtgtttct tggctacttt tttattttta tgtatctaca aaagtttcca
      661 ggggcagaac ctgtgattat taatggaaga acctcaagta ttgtcttaaa tcattgttat
      721 gaaaatggat taaaagagtt catcaaggtg tgattaacaa gttacttttc attacatcga
      781 atttttcaga agttgcttat attggtgatg atattaatga tttatcatgt atgacatctg
      841 tgaagaagcg ggaggaatag tagaatgtcc aaaaaatgcc acaagaaatg agattgaatt
      901 atctgatttt attgtaactc acagtggtgg tgacgaatct gtgatagagt ttattgagtg
      961 gatattggaa tcgatatagt gtagtagtac agaaaaatta tcatacaatt aatactaggc
     1021 ataagaatat tggcatattc tagagttaaa agtaactatt cgaaaaggat aaggttagaa
     1081 ttttttgttt tgccagtgga agaatataaa aagggcattg gaaattaata tattagaatg
     1141 ctaagcggag aaaaaaatat atgaaaagta cgtccttgtg atgggatgga aaaatacact
     1201 tatttcaaac aacgataata taattatgtg aaccgaagta tactcgcttc taattcaatc
     1261 ctcaaatccc gacagtcgcg acttcaaaaa agcgtatagt tttaatgtat ccccatatga
     1321 tccgaaaaat ggtaatttac aattaacagt ttgatgatgt tgaagatgtg gaaatgctga
     1381 aattaccatg atgagaaaca cattaattaa agggaattaa ttaactagaa tataatctga
     1441 ttcaatttga attaagcaaa aaaaggctga gttttaaata atcgcgaatt gtaaaattaa
     1501 gttagaaaaa taaaaaggca tttatggtac actcaaattg tatttccgac gaaagaaaac
     1561 aa
//
LOCUS       DARDHD010000281         1552 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000281 DARDHD010000000
VERSION     DARDHD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.285"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            209..>1552
                     /locus_tag="SDA06_002782"
     CDS             209..>1552
                     /locus_tag="SDA06_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4229999.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL"
BASE COUNT          525 a          230 c          264 g          533 t
ORIGIN      
        1 tgctcaagaa atttatacaa caggtgattt atcttttcat ctttcacgaa tcaaagggtt
       61 atcttctatt tttgaagggc caatcaacta tacaacattt aacaattatg gagatggact
      121 gaactatttt tatccttttc ttacaataat tcctgcagtc gttttttatg gaatttcaaa
      181 taacttaata ttaagttatg ttctatatat ttggttatta aatatttgta cgattctgat
      241 tagtttttat tatggagaaa agttttttaa aaagatagat gctgcttttt tatttagttg
      301 tttatatact tttgcaactt atcgaacagt tgatatctat tatcgatccg caattgcaga
      361 agcaatcgct cttacgatta tcccagctgt tttatattat gcttatggat tgatattcaa
      421 tagagaaaaa aaagtcatcg ctttatctgt cagtataagt ctacttgtgt atactcatgt
      481 cttaactaca ctattgtgta gtgcattgat tgcttgtatg attatagttc gtttgttttt
      541 aagcaagcaa ataaaaaacg atataaaatt tgtggtcgtg aatttagtaa aggctgtttt
      601 tttgacttgt gcattaacta gctatttttg gtatcctatg ttgcagcaga tgaattatca
      661 agaaatacac agtcccttca aaacagattt gcagctagaa gcactttcta tttcagatag
      721 tatcattcaa gcgttaaata acgatattac aacatacact atggggttat taggattggt
      781 tgcgttgatt tttcctttat tcatactgaa agaaatggag aaaaaagaaa aaatcatcta
      841 tgttggcgta gtttctacat gggtatgcgt aactaattta tttccatggt ttttgtttca
      901 aaaaacaccg ataagcttac tccagtttcc ttggcgtatc ttaggtatcc aaatcttttt
      961 ttcttcaatg atcataacgc ttgtttttat gaaaaagact atgaataaaa aatattcatg
     1021 gttaatcatt ggcgctactg tggtgtttct actcgtcacg tctatagcag caaaagaaaa
     1081 ttacgcaaaa gttatccaat caaaacacgg tcatattgtt atcaatgaag aaacactccc
     1141 acgttataca acatcagata tgggaggatt atacgattac gccccaacag aagcgataaa
     1201 agaaaggcct cacttagaaa aacatgaagt aaaagtagga gaatcgtggg aaaaagggaa
     1261 gtataaagct gcagataata acattactta cacagtggca agcaacaaaa agcaaaaagt
     1321 gacagtacca gtatatgctt atttaggtac acaggtaaaa gtaaacggta aagtcgtaaa
     1381 tgactcttat aaaaaacatg gcttagtgaa tgtggatgta aatgctggag aaaataagat
     1441 agaaattaca acaaaatata ctccaactgc tctcatggct ttatggatca gtattctgac
     1501 ttattgttta gtggtgtatt caagttttaa acgatttgca cctaataagt tg
//
LOCUS       DARDHD010000282         1551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000282 DARDHD010000000
VERSION     DARDHD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.286"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..445
                     /locus_tag="SDA06_002783"
     CDS             191..445
                     /locus_tag="SDA06_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4230000.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="SDA06_002784"
     CDS             446..763
                     /locus_tag="SDA06_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4230001.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1551)
                     /locus_tag="SDA06_002785"
     CDS             complement(1397..>1551)
                     /locus_tag="SDA06_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG4230002.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          550 a          231 c          293 g          477 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa g
//
LOCUS       DARDHD010000283         1533 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000283 DARDHD010000000
VERSION     DARDHD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.287"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(288..1199)
                     /locus_tag="SDA06_002786"
     CDS             complement(288..1199)
                     /locus_tag="SDA06_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4230003.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          428 a          283 c          225 g          597 t
ORIGIN      
        1 tttgtgagaa aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa
       61 aaccactcca tttatctact tctacgtgca ttttttgata ttgttttctt tatttaagat
      121 tatacagaaa aatatctagg gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa
      181 gaactaaatc aagtagtaat taaactactg ttgatttagt tctaataaaa agatttagat
      241 gaacagtttt attgatttaa atagttcata ttttttattt ataccgctta tttactactt
      301 gctgtactag aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt
      361 tttacattag cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt
      421 aattttgtgt ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct
      481 ttgttttggt ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg
      541 gtagaagtga tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact
      601 tctgccggaa ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc
      661 gtagtatcag ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct
      721 ttcttcacat cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct
      781 ggatggaaag attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt
      841 agaccttctt gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa
      901 cctgcagatt tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat
      961 tgagaatcga tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa
     1021 acgatcaact gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact
     1081 tcttgttcgc tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta
     1141 cagcctgaca ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt
     1201 tatcaattct cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga
     1261 aaatcatcaa gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac
     1321 acaatccaga aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata
     1381 ctacttcttt tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat
     1441 tcttagaaca aatgttatcg attttgtttt tcttcttccg ctgctgggat attcaaatca
     1501 gacactttta tgttcgattt tccagacatt ttt
//
LOCUS       DARDHD010000284         1522 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000284 DARDHD010000000
VERSION     DARDHD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.288"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SDA06_002787"
     CDS             <1..221
                     /locus_tag="SDA06_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG4230004.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SDA06_002788"
     CDS             218..1237
                     /locus_tag="SDA06_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG4230005.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="SDA06_002789"
     tRNA            1426..1499
                     /locus_tag="SDA06_002789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          495 a          287 c          317 g          423 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg tt
//
LOCUS       DARDHD010000285         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000285 DARDHD010000000
VERSION     DARDHD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.290"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(70..154)
                     /locus_tag="SDA06_002790"
     tRNA            complement(70..154)
                     /locus_tag="SDA06_002790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Leu,seq:caa)
     gene            complement(172..243)
                     /locus_tag="SDA06_002791"
     tRNA            complement(172..243)
                     /locus_tag="SDA06_002791"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Gln,seq:ttg)
     gene            complement(253..325)
                     /locus_tag="SDA06_002792"
     tRNA            complement(253..325)
                     /locus_tag="SDA06_002792"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:His,seq:gtg)
     gene            complement(387..471)
                     /locus_tag="SDA06_002793"
     tRNA            complement(387..471)
                     /locus_tag="SDA06_002793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Leu,seq:caa)
     gene            complement(492..562)
                     /locus_tag="SDA06_002794"
     tRNA            complement(492..562)
                     /locus_tag="SDA06_002794"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Cys,seq:gca)
     gene            complement(608..679)
                     /locus_tag="SDA06_002795"
     tRNA            complement(608..679)
                     /locus_tag="SDA06_002795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Gln,seq:ttg)
     gene            complement(691..763)
                     /locus_tag="SDA06_002796"
     tRNA            complement(691..763)
                     /locus_tag="SDA06_002796"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(728..730),aa:His,seq:gtg)
     gene            complement(766..839)
                     /locus_tag="SDA06_002797"
     tRNA            complement(766..839)
                     /locus_tag="SDA06_002797"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Trp,seq:cca)
     gene            complement(846..926)
                     /locus_tag="SDA06_002798"
     tRNA            complement(846..926)
                     /locus_tag="SDA06_002798"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(890..892),aa:Tyr,seq:gta)
     gene            complement(928..1003)
                     /locus_tag="SDA06_002799"
     tRNA            complement(928..1003)
                     /locus_tag="SDA06_002799"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(968..970),aa:Phe,seq:gaa)
     gene            complement(1039..1111)
                     /locus_tag="SDA06_002800"
     tRNA            complement(1039..1111)
                     /locus_tag="SDA06_002800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1076..1078),aa:Asp,seq:gtc)
     gene            complement(1121..1193)
                     /locus_tag="SDA06_002801"
     tRNA            complement(1121..1193)
                     /locus_tag="SDA06_002801"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Val,seq:tac)
     gene            complement(1209..1280)
                     /locus_tag="SDA06_002802"
     tRNA            complement(1209..1280)
                     /locus_tag="SDA06_002802"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Glu,seq:ttc)
     gene            complement(1295..1384)
                     /locus_tag="SDA06_002803"
     tRNA            complement(1295..1384)
                     /locus_tag="SDA06_002803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1348..1350),aa:Ser,seq:gga)
     gene            complement(1424..1497)
                     /locus_tag="SDA06_002804"
     tRNA            complement(1424..1497)
                     /locus_tag="SDA06_002804"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1461..1463),aa:Asn,seq:gtt)
BASE COUNT          438 a          390 c          340 g          336 t
ORIGIN      
        1 ggaggtaatt acataaaaac aaaaaaaata tagcaactct ctataagaaa gttgctatat
       61 tgataattat accggcggcc ggggtcgaac cggcacgtcc tcaacggaca ctggattttg
      121 agtccagcgc gtctgccaat tccgccacgc cggcaaattt aaaaataata actggggtag
      181 ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc
      241 ccaagaataa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
      301 gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt ctcaataaaa
      361 aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg cacgccctca
      421 acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caagttgatt
      481 tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc agacctctgc
      541 cttaccactt ggctataccg ccattatact attatcaatc tataaaataa agaataaatt
      601 atagaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      661 accgcttggc tataccccaa gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg
      721 ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatggca ggggcagcag
      781 gaattgaacc cacactaacg gttttggaga ccgctgttct acctttaaac tatgccccta
      841 taaaatggag gagagtggat tcgaaccact gaaccctaag gaacggattt acagtccgtc
      901 gcgtttagcc acttcgctac tcctccatgg tggcgcggga cagaatcgaa ctgccgacac
      961 acggagcttc aatccgttgc tctaccaact gagctaccgc gccatatttt aaaattatta
     1021 attgaataac aagaacgacg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag
     1081 gcaggcatgt taacccctac accacggaac cagttcatta tggaggttaa cgggatcgaa
     1141 ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc tccaacattc
     1201 aattaaaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt
     1261 aaccgcttga ccaacgggcc aggtaaattt gctacggaga gtaagggatt cgaacccttg
     1321 agacagtttg gcaccatcta catgatttcc aatcatgctc cttcggcctc tcggacaact
     1381 ctccagagtg aaagaagctc aatactcttt ctcaaaatga gaactccggc agtaggactc
     1441 gaacctacga catcatgatt aacagtcatg cgctactacc aactgagcta tgccggaata
     1501 acat
//
LOCUS       DARDHD010000286         1500 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000286 DARDHD010000000
VERSION     DARDHD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.291"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            403..1122
                     /locus_tag="SDA06_002805"
     CDS             403..1122
                     /locus_tag="SDA06_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4230006.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1124..>1500
                     /locus_tag="SDA06_002806"
     CDS             1124..>1500
                     /locus_tag="SDA06_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4230007.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFG"
BASE COUNT          521 a          181 c          264 g          534 t
ORIGIN      
        1 cctattcact attaataacg ataaacatgt ataaaaagac acactacgaa aaaagttaaa
       61 tggagtttac tgattttcat atttgtatat ggaacagaca tagtcttgaa taatttttat
      121 aaatgttttg atgcttatca acttattcaa cccgctatcc acttcaagta taatttttcc
      181 atacgccaat aaagtagttt taggagcatg ttctcatttg agaacgctcc ttttttatat
      241 attttgaaag ctacttacta tacgcgtagt ttcaaatagt tttgtcattc taatcttcag
      301 caacagaaaa aaatctaaac tataaaattt agggttgact aaaatataat ttttgtatac
      361 catatgatcg tgataatcat tatcatttag aaggagaaag ttatgttaga agtgaaaaag
      421 cttaatgttt gttataacga ttttttagca ttgaacgaga tttctttaaa aatacaagaa
      481 ggtgctttaa caggtattat tggtccaaat ggtgctggaa aatccacatt gcttaaagca
      541 atgttaaata ttatccctca tcagggagag gtctccatta ataatgaaga tatcaataaa
      601 aaactggtga agattgcata tgtagagcaa aaagctgata ttgattttac ttttccaata
      661 aaagtaaaag aatgtgtatc tatgggaaca tatgccggaa tgaaagtttt tcaaagaatt
      721 aaaaatgcag aatggcaaag agtttcaaag gctttggaaa aggtcgatat gggaaaatat
      781 tctaatcatc aaataggaga attatctgga ggacaatttc aacgtgtact tttagcaaga
      841 tgtcttgctc aaaatgcgga tttcatattt ttagatgaac catttgtagg gatagactta
      901 gttagtgaac gaataattat ggatacgtta aaaaaattga aacatcaggg aaaaacagtg
      961 ctaattgtgc atcatgattt aagcaaggta aagaagtatt ttgataatat tattcttttg
     1021 aatcgaaatc taattgctca cggaagtgta gaatctgtat ttaatgaaga aaatttaaaa
     1081 aaagcctatg gagatactat atttattgga gagggggaat aatatgcttc aaaattttat
     1141 taatggtctg tatgactttc actttttaca gaatgctcta attacatctg tagttatagg
     1201 gattgtttct ggaacagtag gttgttttat tattttaaga ggaatgtctc ttatgggtga
     1261 tgctatttct catgctgttt tgccaggagt agcaatttct tatattttag gtattaattt
     1321 ttttattggt gcgattgtat ttggtctatt agcttctatc attattactt ttataaaaag
     1381 caatagtgta ataaaaggag atacagcaat tggtattact ttcagttctt ttttagcact
     1441 aggagttatc cttataggag ttgcaaatag ctcaacggat ttatttcata ttttatttgg
//
LOCUS       DARDHD010000287         1500 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000287 DARDHD010000000
VERSION     DARDHD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.292"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     regulatory      187..266
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            264..1457
                     /locus_tag="SDA06_002807"
     CDS             264..1457
                     /locus_tag="SDA06_002807"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG4230008.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT          470 a          243 c          346 g          441 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
//
LOCUS       DARDHD010000288         1484 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000288 DARDHD010000000
VERSION     DARDHD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.293"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SDA06_002808"
     CDS             286..693
                     /locus_tag="SDA06_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230009.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SDA06_002809"
     CDS             707..1108
                     /locus_tag="SDA06_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230010.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="SDA06_002810"
     CDS             1110..1481
                     /locus_tag="SDA06_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230011.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          472 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atga
//
LOCUS       DARDHD010000289         1455 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000289 DARDHD010000000
VERSION     DARDHD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.294"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..423
                     /locus_tag="SDA06_002811"
     CDS             <1..423
                     /locus_tag="SDA06_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4230012.1"
                     /translation="GVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVL
                     LTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGY
                     SFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
     gene            420..1370
                     /locus_tag="SDA06_002812"
     CDS             420..1370
                     /locus_tag="SDA06_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4230013.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1385..>1455
                     /locus_tag="SDA06_002813"
     CDS             1385..>1455
                     /locus_tag="SDA06_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG4230014.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRK"
BASE COUNT          546 a          188 c          274 g          447 t
ORIGIN      
        1 ggcgtttcgg ttttagtgtt ggttatagta gttctatttt ttaaagaatt attaatcact
       61 tcttttgatc cattaatggc aaaagcaata gggatgaatg tcagttttta tcattatcta
      121 ttgatggttc tattaacttt agtagcagta acagctatgc aaagtgtggg aactatatta
      181 attgttgcaa tgttaattac accagcagct acagcatatt tatatgcaaa tagcttgaaa
      241 actatgatat ttatttctgc aacagtcgga gcaagttcat cattgcttgg attgtttatt
      301 ggttatagtt ttaacatagc agcaggatca agtattgttt taacagcggc attcttattt
      361 gtaattagtt ttctaatctc accaaaacaa aaatttttaa gaaagaagga gagatcgtta
      421 tgatgagaaa atggaaagca gtactgggaa gtctggggat tttgattgct ctttttatat
      481 tcggggcatg ttcaataaat agtaaagaca aagataaagt ggcttcgaac gaaaaattaa
      541 aagtagtagt tactaattcg attttagcag atattaccga aaatatagca aaagataaaa
      601 ttgatttaca cagtatcgta cctattggga aagatcccca cgaatatgaa cctttgcctg
      661 aagatgttca aaaaacttca aaagcagatt tgatttttta taacggtgtt aacttggaaa
      721 ctggaggaaa tgcttggttt acaaaattag ttaaaaatgc gaacaaagag gaaaacaaag
      781 actattttgc agcaagtgat ggcatagatg ttatttactt agagggtcag agtgagaaag
      841 ggaaggaaga tcctcatgct tggttaaatt tagaaaacgg tattatttac gctaaaaata
      901 ttgaaaaaca gttagcggaa aaagatcctg ataataaaaa attctataaa gaaaatctag
      961 ataagtatat tgaaaaattg gattctctag acaaagaagc taaatctaaa tttgcttcaa
     1021 ttccgaatga taaaaaaatg attgttacaa gtgaaggatg ctttaaatat ttctcgaaag
     1081 cgtataatgt gccttctgct tacatttggg aaatcaacac tgaagaagaa ggaacaccag
     1141 atcaaataaa acacttagtt gaaaaattac gcacaacaaa agttccctcc ttattcgtag
     1201 aaagcagtgt ggatgataga ccaatgaaaa cagtatcaaa agataccaat attcctatct
     1261 attcaacgat ttttactgat tcaattgcag aaaaaggaca agatggtgat agttactatg
     1321 cgatgatgaa atggaacctg gataaaattg ctgaaggcct ttcgaaataa tttggagggt
     1381 aggtatggta caaattaaaa cgactactat actaatttat ttactagcaa ttcaggaatt
     1441 atcaaaaaga aaaaa
//
LOCUS       DARDHD010000290         1449 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000290 DARDHD010000000
VERSION     DARDHD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.296"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SDA06_002814"
     CDS             131..505
                     /locus_tag="SDA06_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4230015.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SDA06_002815"
     CDS             complement(619..1248)
                     /locus_tag="SDA06_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4230016.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          487 a          221 c          255 g          486 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaa
//
LOCUS       DARDHD010000291         1444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000291 DARDHD010000000
VERSION     DARDHD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.297"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..999
                     /locus_tag="SDA06_002816"
     CDS             127..999
                     /locus_tag="SDA06_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4230017.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1295..>1444
                     /locus_tag="SDA06_002817"
     CDS             1295..>1444
                     /locus_tag="SDA06_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HEG4230018.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGT"
BASE COUNT          455 a          270 c          319 g          400 t
ORIGIN      
        1 ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catgttacta aataaaaaaa
       61 taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa
      121 atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc
      181 gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca
      241 atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt
      301 ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg
      361 accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg
      421 atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc
      481 agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt
      541 gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc
      601 gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta
      661 gaagggatgg ccgctggacc cgccatcgaa aaacgcttag ggaaaaaagg acaagaggtt
      721 gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat
      781 acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc
      841 catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca
      901 cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt
      961 tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca
     1021 tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg
     1081 cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg
     1141 aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacacg
     1201 ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact caaatcgtaa
     1261 tacccgctaa agcatacaag gagagtgaac ataaatgact tatacccatc ttacaccaaa
     1321 cgagcttgta atgatagaag catattttca tcaagaaact ccggttgcta tcgttgcgaa
     1381 gcagcttaaa cgtggacgcc aaacaattta caatgtctat aactttctca aatgtggtgg
     1441 aaca
//
LOCUS       DARDHD010000292         1442 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000292 DARDHD010000000
VERSION     DARDHD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.298"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..770
                     /locus_tag="SDA06_002818"
     CDS             <1..770
                     /locus_tag="SDA06_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4230019.1"
                     /translation="EKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFS
                     GFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMIT
                     QSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSL
                     SGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKIS
                     QLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1000..1230
                     /locus_tag="SDA06_002819"
     CDS             1000..1230
                     /locus_tag="SDA06_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEG4230020.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          500 a          208 c          229 g          505 t
ORIGIN      
        1 ctgaaaaaaa agtcgctgat tttattttaa attctgggaa aactattatt tatagtacga
       61 tgagtgatat taagaaaaac acaaaagttg gcgatgctac tattattcgc ttttgtcaaa
      121 aaattggttt ttctggtttt tctgatttaa aaattgaaat tgcaaaggaa gactttagtc
      181 aaaaaaaaga acatccgact agtgtaaagt actatgatga aattgctaat agtttaacag
      241 aagctctcta ttccactatt aggcttttag acgaagaaaa attgaatgaa gccattaaaa
      301 tgatcactca atcaaaaaac atatatatat ttggagtggg atctagtgga aatactagtc
      361 ttgatttaga aaatatgttt cttcgcgtag gagttcaagc aaaagctgta ctagaccctc
      421 attttcaagc ccaagtcgct tctttattaa cagttaatga tttagtaatc attttttctt
      481 tatctgggaa aacgaaagat acttatgatt cattgaaaat agctaaaaaa aatggcgcaa
      541 aaattattgc tataactaac tatattcatt cacctatagg aaagtctgca gacttagttt
      601 tacaaacagc cattgaagaa tttcttaatg gtggatcttt agctggaaaa atatcacaac
      661 tttatatttg tgatctatta gttcatggtt atgaacaaaa caataatatt gattcagttg
      721 aattaagaga aaaagttttg cgctctatta ttgataaaag tattgagtga actaccccca
      781 cttagctaaa cctaacggtt tttaacgatt gaagtaggca attcttggga tgagagcata
      841 cttgatagct tattttttac taatagcggt ggctcttatt gaccaaatat ccctgtagtt
      901 cctacggttc ttgattttat ctaaagatat ttttatcgtg gaagctattg atcaacttga
      961 aagaaaaacg tgtagctcat tatcactagt ttatcgagta tggatgaagc aatggatcat
     1021 cctctcttaa ataaatttat taaggatctg atccttcaaa ttttttccat gatcgatgag
     1081 caggaaccta ctgaatctaa acgacatcgg gcacaaggaa ttattaagat tgctaagcga
     1141 atgttttcta taaagggtgt tctaagctat acagtgttaa ttcaaaagat tcccaatcat
     1201 cttgtttata agaacaacgt tgaagattaa aaaagtgttt caatgctaaa attgcaaaat
     1261 acttcaatgt tatgagacaa actgtctata agaatcaatt tccacccatt tcatcacttg
     1321 ttaaaagttt atgatccttt tgatttttat attaatttat ctgtatttaa tacagatttt
     1381 atttattagt tcatctgcct attgtatttt attttgttgc cttaccatct gaagtaaata
     1441 ta
//
LOCUS       DARDHD010000293         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000293 DARDHD010000000
VERSION     DARDHD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.299"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SDA06_002820"
     CDS             105..1406
                     /locus_tag="SDA06_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4230021.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARDHD010000294         1424 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000294 DARDHD010000000
VERSION     DARDHD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.300"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SDA06_002821"
     CDS             complement(150..1337)
                     /locus_tag="SDA06_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG4230022.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DARDHD010000295         1398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000295 DARDHD010000000
VERSION     DARDHD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.301"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(96..980)
                     /locus_tag="SDA06_002822"
     CDS             complement(96..980)
                     /locus_tag="SDA06_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4230023.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1075..>1398)
                     /locus_tag="SDA06_002823"
     CDS             complement(1075..>1398)
                     /locus_tag="SDA06_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230024.1"
                     /translation="IHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLI
                     AIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMF
                     YLVKK"
BASE COUNT          435 a          303 c          224 g          436 t
ORIGIN      
        1 tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt
       61 gttctcccgc caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag
      121 ggcaatactt tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc
      181 aattcttcct catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt
      241 ttcgtagatt catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa
      301 gaagaatctt ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt
      361 aatgtttcaa aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt
      421 tcttccttct ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt
      481 ttgattcctg acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca
      541 tcattgattg attcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt
      601 atttttccaa tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca
      661 ggttctagcg gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat
      721 ccgaaatttg gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca
      781 tctccgccca tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt
      841 tttgcatccc ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat
      901 tttgctatcg ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc
      961 actaataaaa cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga
     1021 tagcataaag aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt
     1081 tttgacaaga taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga
     1141 aataaaagaa aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata
     1201 aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct
     1261 tatagcgatc agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat
     1321 gtcacggcct tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa
     1381 aacggaaaat tgatgaat
//
LOCUS       DARDHD010000296         1390 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000296 DARDHD010000000
VERSION     DARDHD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.302"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..630
                     /locus_tag="SDA06_002824"
     CDS             <1..630
                     /locus_tag="SDA06_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4230025.1"
                     /translation="LNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQIDKVYVYCSNP
                     DIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTTAPFISEKSIE
                     KGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLYEETSGFYIYN
                     RNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQGS"
     gene            649..>1390
                     /locus_tag="SDA06_002825"
     CDS             649..>1390
                     /locus_tag="SDA06_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG4230026.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNA"
BASE COUNT          525 a          174 c          271 g          420 t
ORIGIN      
        1 ttaaataaca aaagattgcc acaaaaaaac acgaaagctt ttaccaatgg aaaaccattg
       61 tgttattata tactttcaac acttttaaaa gtaaaacaga ttgataaagt gtatgtctat
      121 tgtagtaacc cagatattaa agaatttata ccagaaggtg taacattttt aaagagatct
      181 agttccttag atcaggatac aacgagtatt acacaggtat tagccggttt cacaaaagaa
      241 gtagaggctg atgtatatgt aatgacacac acaacagcac catttattag cgaaaaatct
      301 attgaaaaag gactagatgc cgtgctgaca ggagaatatg attctgcatt tgctgcaaaa
      361 aaattgcaag atttcttatg gaaagacggt gaaccattta attatgaatt ggacaatatt
      421 cctagaacac aggatttgcc gcctttatac gaagaaacta gtggatttta tatttataat
      481 cgcaatgtga tgaccgagtt gcatagaaga attgggaaaa aaccttgcat agttgaagta
      541 ggtgagattg agtcagttga tattgatgaa gccgaggatt ttatgattgc tgatgcgatt
      601 tataatcatc ttattttaca aggtagttaa gacaggggga aggttaggat gaacaatgta
      661 cagattttag attgcacact tagagatggg ggctattgta acaattggga atttggattt
      721 gataatacaa aaaaaattat ttctggactt atagaatcag gaattgagat tatagaatgt
      781 gggtttctca caaataaaac agtatacaat aaaaatgtta caaaatatac gaccttagaa
      841 gaattagcac agataattcc tgaaaaaaga gaaggaaaat tatttgtagc aatgatgaac
      901 tatggcgaat ataatataga ggatcttcca gattatgatg gttcatctat tgatggaatt
      961 aggattgcgt ttcataaaaa aaattgcaat gaagcgttga aattatgtcg tgagataaag
     1021 aaaaaaggct acttagtttt tgttcaagct atggtatctc tcaattatac agatgaagag
     1081 tttttagcag taataaaaaa tgtgaatcag tttaaaccat acgcttttta tattgttgat
     1141 agttttggaa tgatgaaagg aaaagattta actcgtcttt tttacatgat tgagcataat
     1201 ctagaaaaag atatacagat aggttttcac tcacataata acatgcagct tgcatattct
     1261 aatgcacaaa aacttataac cgtacaaaca aatcgaagcc tgattattga ttcttcggtg
     1321 tacggaatgg gaagaggagc aggtaatctt aacacagaac tatttgtaga atatttgaat
     1381 gataatgcag
//
LOCUS       DARDHD010000297         1383 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000297 DARDHD010000000
VERSION     DARDHD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.303"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            379..711
                     /locus_tag="SDA06_002826"
     CDS             379..711
                     /locus_tag="SDA06_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230027.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1007..1162
                     /locus_tag="SDA06_002827"
     CDS             1007..1162
                     /locus_tag="SDA06_002827"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG4230028.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          487 a          244 c          244 g          408 t
ORIGIN      
        1 atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
       61 gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg aatccagaac
      121 tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      181 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      241 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      301 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
      361 cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact aaagatttca
      421 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
      481 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
      541 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
      601 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
      661 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
      721 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
      781 aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac agaaccaaat
      841 tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc gcctctattt
      901 gtccacctat attgacttag caactcgtat tctacgttac tatatggtag attcacacat
      961 gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat
     1021 tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt tgaacgacca
     1081 ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag taattgaagc
     1141 gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga
     1201 gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac
     1261 aaaatttatg atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct
     1321 agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga
     1381 cac
//
LOCUS       DARDHD010000298         1382 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000298 DARDHD010000000
VERSION     DARDHD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.304"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(888..1301)
                     /locus_tag="SDA06_002828"
     CDS             complement(888..1301)
                     /locus_tag="SDA06_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG4230029.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          509 a          234 c          201 g          438 t
ORIGIN      
        1 ttatacttca aaaaagataa cacccaactt tcaaaaatat gttttggaag ttgggacaaa
       61 cgagttaata cattttaata cattattttt tattcaaaaa aaatagcaca atggcaaaga
      121 tcgtaagaga gatcataagc cccaatgaat gactaaaaag aaaagcagaa tcacatgcca
      181 tatcatccca tatttcttca agaaaataaa taagcgggaa aaagggaagg aatagtagaa
      241 catcgatcca gcaccatttt tttcttcttt tgaaataaca taaaacaata aacatgaacg
      301 tgcagggaag taaaaacaaa gctttactca taactaacaa aataccagct ccttttattt
      361 ttttctcccc cacattcaaa gagtaacaat ggtatgtact taataataga aaaatggatc
      421 aacttgtatg aataacagct gaaaagatct gcaccaaata aaaaacttcc agccaaaact
      481 gactggaagt ttattttttt ctgaaatatt ctaacacaaa tatgaaaaag atggccgaag
      541 ctaataagcc gatttcgtag caaaacaatg agataccaga gtcgtaataa taaaatcctc
      601 taatcttatg gataaaatag atcatcggac aagcagataa gagtaagtat ttgttaggcc
      661 ttttgaataa gtaaaagaaa gcactggtca ttatgataaa tggtatccaa aacaaaagtt
      721 tacttaagac aatcaaatga tcaactccca tattttttac caagagagat cataacatct
      781 ttatcgtaaa aacaaaatat ttttttgctc attttgaaaa tttaaacata ctcgttctta
      841 tccaaatata taaaaatcgc ccctcattca tcgagaggcg ataagttcta atttaactca
      901 attttttttg tttcttttgg ctgataagct gttttaggta ataccaattt cagcacaccg
      961 tttttaaagg ttccagtgat tttttcttca tcaacattag gaagatagaa tgaacgtttg
     1021 taagagaccg aatgtctttc tttccgtaca tagttgccat cttctttatc ttctgtatgg
     1081 gactcatgat tggcggagat cgtcaaagta ttatctgaat attcaactgt cgtgtcttct
     1141 ttgtcacagc caggcatatc agcagttagt ttgtattctt tttcattttc gactaaatct
     1201 acttttggat aacttgaaac acctaaaaaa tcattgaatg ctggactaaa cacgtcattg
     1261 aaatcaggga acatgtcgcg aatgctaggt acatttgcca tttttgatca tcctttcaat
     1321 aagtattttt tatttatccc tatttacgta ataactctat tttgaaaaaa atgcaagtat
     1381 aa
//
LOCUS       DARDHD010000299         1380 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000299 DARDHD010000000
VERSION     DARDHD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.305"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..826)
                     /locus_tag="SDA06_002829"
     CDS             complement(<1..826)
                     /locus_tag="SDA06_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG4230030.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQT"
     gene            complement(832..>1380)
                     /locus_tag="SDA06_002830"
     CDS             complement(832..>1380)
                     /locus_tag="SDA06_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4230031.1"
                     /translation="GISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLIIG
                     TGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQF
                     ADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYN
                     IQTAYKILESKYYELFELIQRG"
BASE COUNT          433 a          256 c          158 g          533 t
ORIGIN      
        1 atgtctgtgc tatttcatga tttattctct gtgctaaaac aatcatataa tctatcatca
       61 aataatccac catcatatca ttatttttcc aataatttaa aaaaaagtct ctaattatag
      121 caaacatcca tctagactct tgattacatg ctaatgaata acccgcaaaa tatcctgatg
      181 ctacggatgc gtgagcataa tcaggcctcc taatagacca aatatcgata tcaaaatact
      241 catctggtat tggttttgta caataaaatg tggcgtctaa ccacaaacct ccatgttttg
      301 ctagcaaaga taatcttaat aaatcagaat aatttgttct cgtaatgatt cctttgtttt
      361 tcttttcttc cacccatttt ggtatatcaa catattgtct ataattatct tctgttaaaa
      421 ttataacagt atgtttccct gcatttcgtt ttattgattt tacacaagct tttacaattt
      481 ccggcgattg atccaatccc tgccaccaac aaacccaaat gcagttagaa tatggtgatt
      541 ttgattgatt aattatttga ttttgactat aatcatatgt tttcaaaaaa tctgaaaaag
      601 tcttttcata atacttcagc attacctcat gtttttttaa taaatgttcc ttctgttttt
      661 tactttcatt atatccatta cgattcatga cttgaatatc aattttagct tgtaacgtgg
      721 caaatgcttc cttccaagat gttatctttg caattcgtct tgttgcattc aattcttcgg
      781 atatgcgatg tataattctt tttatacttg aatgtttctg tgtcatattc tttaacctct
      841 ttgtattaat tcaaataact cataatattt tgattctaag attttgtatg ctgtttgaat
      901 attataatct gcctttatta aaatcgaacg ttctgaatct cgatgagaga tatctacatt
      961 tgcaatctct ctcgcccatt cttttgccga ttgatttaaa cttatgaaat tggtcttatc
     1021 actgaaagca acttctgtag gaactttctc cgaaaagaaa cacggtaaat ctgcaaattg
     1081 agcttcaata cccactaacg gaaccccttc aaataatgat ggcaacatta atacgtcaaa
     1141 tgcttgataa aatttttcta tatcatttct atttcctaaa aatttaatat catcatctat
     1201 gccacttttc tttgcataat tttttatttc ttcttccata tcaccagttc caataattaa
     1261 taatacggaa ttattcttaa tagcgtgaaa ttctttaaaa atttcaatca aaaaacgatg
     1321 atttttagaa tatgaaattc ttccaatatg tcccattaca aacgtctgac ttgaaattcc
//
LOCUS       DARDHD010000300         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000300 DARDHD010000000
VERSION     DARDHD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.306"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SDA06_002831"
     rRNA            <1..1355
                     /locus_tag="SDA06_002831"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARDHD010000301         1345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000301 DARDHD010000000
VERSION     DARDHD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.308"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            183..557
                     /locus_tag="SDA06_002832"
     CDS             183..557
                     /locus_tag="SDA06_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4230032.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            562..990
                     /locus_tag="SDA06_002833"
     CDS             562..990
                     /locus_tag="SDA06_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4230033.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1081..>1345
                     /locus_tag="SDA06_002834"
     CDS             1081..>1345
                     /locus_tag="SDA06_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230034.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDE"
BASE COUNT          489 a          211 c          239 g          406 t
ORIGIN      
        1 agacacagaa atgcctagta atttacattt cagagtttgc cgaccgtgaa gtgtttatta
       61 gttatcaagt ctttttagct aatattttag cttacgaaaa cagtatacta acacttttat
      121 tagttgtcaa cacctttaac taattttttt attagtgttt tcgtagcttg tgaggtgcga
      181 aaatgaattt actagataga ataaaggaat tagctcataa aagaggcata agtataactc
      241 aattggaaga agaattaaat atacctaaaa acacgattta tcaatggaaa aatagaactc
      301 ctagtactaa aagactacaa cttgtagcag attattttaa tgtaactact gattatttac
      361 tcggtagaaa ccaagttcct gactgggcaa caaaagatga agtagttgaa cttgataaat
      421 tactagattc aaatgtcaat atgtcttatg gcggagaaac actgacgcct gaacaggtcc
      481 aacgcgtaaa agatatcctt attggaacct tctgggatat tgtgaaagaa gacaaagaaa
      541 aaggcaaaaa gatgtgagct tatggagatg gatacgatta atttagtcga ggagttgaag
      601 cggaaatacc agtccgctaa tcctttttat atttgtgaaa agatgggcat taaaattcaa
      661 tacgttcctt ttatcgagaa tcccaaagga cagtttcaag aaattagagg tcgtgcaata
      721 atcttcttaa atgatgaact gagagactct gaggaaagat tctacatttg cgctcacgaa
      781 ttaggtcacg ctatttttca tcgtggctta tccagttact atgtatcaac aagaacatct
      841 agaagcaaat ctgaaagcga agctaattgc tttgctgcta atctcattgt ttctctatac
      901 aaagaagaca atgatcaata tcctaaacga attgaggaat taaagaatct ttacggactt
      961 ccagaaagcg cttatcgctt tcttatataa tgtaaaaaaa gtccgtgctg caacacggac
     1021 taaaaacctt atttctaaga tcctacaaaa aaatcatatc atagaagtga ggaattaaaa
     1081 atgaaaaaaa ttgtttttgg gttgcttttt ttaggcttgg tattaagcgg atgctccact
     1141 agtcctaatt ctgctagcac taaagaaaat actgtttcat caacatctga atatgaaaca
     1201 aaaaaactaa cttcatcaga cgttcataaa ataaaagttg gtgatgattt aactcaagtt
     1261 tatcagacac ttggttcacc gttacgtaaa tggacaaatg attttgttta caaagaatta
     1321 gacaatgcta tcacacggga tgaat
//
LOCUS       DARDHD010000302         1341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000302 DARDHD010000000
VERSION     DARDHD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.309"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(313..927)
                     /locus_tag="SDA06_002835"
     CDS             complement(313..927)
                     /locus_tag="SDA06_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4230035.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          433 a          266 c          222 g          420 t
ORIGIN      
        1 tctttcatca acgtgtatta agagacaaga aagaatccat atggattctt tcttgtctct
       61 ttttccacta ctttttattt ttctgacact acatattctt ataactcatt tctcataact
      121 gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca agcttaaaat caaagatgat
      181 ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt
      241 ttcttttttt caaacgctcc atttttatgt ggaacattat ttttttaagg aactttgtca
      301 acatgtggat aactattttc cacttgtgtc ctggactagc tgttttgctg taaaatacaa
      361 aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg ctggatctag
      421 atttggcaaa gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc
      481 aaattcaaat aatcgcaaac cactgctatg ttgcgatttg atacttcgtg acaaatcaaa
      541 tccagtaaca atgattaatg gatgaccatt ttctttgatc cactgagcta gttcgatgat
      601 tgccgggttt cttccttcat tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag
      661 ctgatcaata attgcctgtc ctgcaccttc tagcgtttca tagacacctc cggctggatt
      721 ttcaatcaat atacttggta caagatccgg ggtttgatcc caaaattcaa atgctacacc
      781 acttagtgtg cggctagcat atatctgcaa tctaccacct tgattaattt gtttcgcgat
      841 ccaagtgcca gcttgaagca tcagcggctc ttctatcctt aatttctctt ccaattggtg
      901 ataatagtca aaataacata aactcattta tctctcccgc ctagctatac ttgacatgaa
      961 tagaaacagc tgcctaatct aaggaagtac ataaaaaaac agtagttaga cagtaacaca
     1021 tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc aataccttga
     1081 catagcaaat aaaacaacgt gcagaccatg gaaaatatct acataggggt agaattttca
     1141 ccctagatta ggtacaataa attaggtcga tgcactgcat tgactatggc aaaatgatcg
     1201 gtcagggtgt atgtgtgtga ctggtcattc cctgattctt agcgccaact aagggtcagg
     1261 gagccttttt ttattcagct gtttctatct gaatttattg taagtaatct cttatccaag
     1321 tgcaagcaag aaaatacaaa a
//
LOCUS       DARDHD010000303         1323 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000303 DARDHD010000000
VERSION     DARDHD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.310"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            216..1256
                     /locus_tag="SDA06_002836"
     CDS             216..1256
                     /locus_tag="SDA06_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4230036.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT          454 a          150 c          307 g          412 t
ORIGIN      
        1 cttaatatgg gatgggatat ttggattagg caaaaggttt ttagataggg atttttggat
       61 ttggtttata attgcagttg tagttgtgtg cataatttgc atggctatcg atgttgtaag
      121 agaaaaattt gaaagacaga tttttaaaat taagagtgta aagaacctgt gtaaacttat
      181 gaatcatgtt ataaatatat acccataatt aataaatgag gagaaaagaa gtgaaagtta
      241 tatcattcgg agaattatta ttgagacttg cagcacccgg ttattcaaag ttatttcaaa
      301 gagatagttt ggagactaca ttctgcggag gggaagctaa tgtagcggta tccttagcaa
      361 tatttggata tcaatcagaa tttataacaa agcttccgga ttcagatgtt ggatatgcgg
      421 cagctaatgc tttgcgttat tttggagtaa atgttgataa tattatattt ggagagggac
      481 gcatgggact gtattattta gaaaaaggag cagctcagag accatctaaa gtaatttatg
      541 acagaaaatt ttctgcaatt tcgttagctg atagtgatga atttaagtgg aaggagatcc
      601 taaacgatgc aaattggttt cattggacag ggataaatcc agcattgtct gagaatctct
      661 ttgagacgtc cttagatgca tgtcaggctg ccaaaaaaag aggtataaca atttcttgtg
      721 atttaaatta tcgatccaag ctttggacat cagaggaagc aaaagaaaag atggaacaga
      781 tactaccatt ggttgatatt tgtatttgta atgaagaaga tgcagaaaaa gtgctaggaa
      841 ttaaagctga gcatacaaat attaatagtg gagaattaaa taaagatggc tatgtgcagg
      901 tggctaggga gattcacaag aagtttggat gtaggtatgt agctacaact ctgcgtagaa
      961 gttattcggc aagtagaaat ggttggagtg caatgttata cgaagcagaa aaagataaag
     1021 cgtatttttc cacagaatac aacatacaaa ttgttgatcg cgttggtggt ggagacagct
     1081 tttctgctgg tataatatat gcgttatgtg aaaatatgtc cggacaaaag gctattgaat
     1141 ttgcgactgc atctagttgt ttaaaacata ctatagaagg tgattttaat cgaactacaa
     1201 ttgaggatgt atataattta atgaatagcg gtggaaatgg tcgcgtgttg cgttagtcaa
     1261 catataaaga taaatcacag taatttttat ggtgattatc tgtcttagta gtgattagaa
     1321 taa
//
LOCUS       DARDHD010000304         1297 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000304 DARDHD010000000
VERSION     DARDHD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.311"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..717
                     /locus_tag="SDA06_002837"
     CDS             <1..717
                     /locus_tag="SDA06_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG4230037.1"
                     /translation="LSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPV
                     LLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGY
                     SDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIF
                     LGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYI
                     GKKVKRPISADSVLLEEDVE"
     gene            714..>1297
                     /gene="neuC"
                     /locus_tag="SDA06_002838"
     CDS             714..>1297
                     /gene="neuC"
                     /locus_tag="SDA06_002838"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG4230038.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKY"
BASE COUNT          437 a          170 c          276 g          414 t
ORIGIN      
        1 ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga
       61 tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat
      121 gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta
      181 gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt
      241 tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt
      301 gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct
      361 aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct
      421 tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt
      481 ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga
      541 aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt
      601 acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa
      661 aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa
      721 aaatacttat agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga
      781 atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata
      841 aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc
      901 aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa
      961 atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata
     1021 gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata
     1081 ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa
     1141 tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg
     1201 aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata
     1261 gattacctag ttttagagat ataaaagaaa aatatca
//
LOCUS       DARDHD010000305         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000305 DARDHD010000000
VERSION     DARDHD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.312"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SDA06_002839"
     CDS             <1..1244
                     /locus_tag="SDA06_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG4230039.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARDHD010000306         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000306 DARDHD010000000
VERSION     DARDHD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.313"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SDA06_002840"
     CDS             <1..848
                     /locus_tag="SDA06_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4230040.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARDHD010000307         1265 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000307 DARDHD010000000
VERSION     DARDHD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.314"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..1015
                     /locus_tag="SDA06_002841"
     CDS             8..1015
                     /locus_tag="SDA06_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4230041.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..>1265
                     /locus_tag="SDA06_002842"
     CDS             1027..>1265
                     /locus_tag="SDA06_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG4230042.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQS"
BASE COUNT          501 a          164 c          237 g          363 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
     1261 caatc
//
LOCUS       DARDHD010000308         1255 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000308 DARDHD010000000
VERSION     DARDHD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.315"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(839..>1255)
                     /locus_tag="SDA06_002843"
     CDS             complement(839..>1255)
                     /locus_tag="SDA06_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG4230043.1"
                     /translation="KRVEEVDRTPAENLAYQVGWTNLILKWENDERNGLSVKTPSDQF
                     KWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDLFTAHKRKWADE
                     ATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          388 a          250 c          192 g          425 t
ORIGIN      
        1 gtatttagat aacacgaaag aataaaaata acagctgata gtaaagtttg ttgacgattg
       61 ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa
      121 agacctttta catcttgcag aaatcggtac tgggattaaa ctaagcaata aaaactaatg
      181 ttcccctcat tctttgaact tctactattt gatttattta ttctgataaa tgacgatttc
      241 accttcagga tcttcaaatc ccgtatagaa tttatttaag atctcttcag tatcagtaat
      301 aggataggcg ataggccaca taccctttga tggttagaat aaactgctca aaatctgtat
      361 cgtatgttgg ctttagcata ttaacaaagg ctatctccaa attataaacc ataagcgttg
      421 aatcgttctt aacaatttca attcttccta acccaaagtc agaagatata attaccggac
      481 ttgccttacc cttcatgtgg gttgttgaga ttccatattt aaagggaatt gtcggtacta
      541 tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag tctcctaagt
      601 ccctttgtag cggatatggc accataatct cattttatcg aagtgagtca tggtttatct
      661 gttggccaac caaaattgat acaatctaac gatgcaccct ctacggcatt ctaggggagt
      721 gcacttgcat tcacccaata aaaaccggca gttagcaaag aacttatgta gtcgtatgtt
      781 cttactgtta acatacaaga ggtctccttt ccacaattgc ttagcagtaa acgggaattt
      841 acaaagctaa tctcttccat tttcttatct ttgttctaaa agtcccaaat ggagcaactg
      901 tatttacatg aataaattta tatacttccc atacagccgt tttggttgcc tcgtctgccc
      961 attttctttt gtgtgcagtg aatagatctt catcactcat tgaatcaatc atcatgttaa
     1021 tatcatcaat attacgattg agaatttcct ctaattcttt caatggtaga tgtgcatagg
     1081 tatttgtaaa ccacttatat aactctccta actgattcca tttaaattga tcagaaggcg
     1141 ttttaacaga taatccattt ctttcatcat tttcccattt tagaattaaa ttcgtccaac
     1201 caacttgata tgctaaattt tcagcaggtg ttctatccac ttcctcaact ctttt
//
LOCUS       DARDHD010000309         1242 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000309 DARDHD010000000
VERSION     DARDHD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.316"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1133..>1242
                     /locus_tag="SDA06_002844"
     CDS             1133..>1242
                     /locus_tag="SDA06_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230044.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          439 a          209 c          189 g          405 t
ORIGIN      
        1 tttatatgtt ctcattatat tacctccata aattcctaat tgtctccttt aattcatcta
       61 taatttcatc cacgatattt taatccgttc tacaatgcat atctgatatg cattgactgt
      121 ctaactcata tgtgaatgat ttataaaata tcccaacgtc tcatctaaag aaatgttgat
      181 cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc ggactggttt
      241 gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt actaataaaa
      301 actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag ttcacaaaat
      361 ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg attacagcat
      421 cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat tgacgatgac
      481 acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca actcgaagaa
      541 aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac tattaaaggt
      601 caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt tgcacaatgc
      661 atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg tgtaatgaaa
      721 cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa taattatgtt
      781 aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag attgtttaag
      841 gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc tgttaagttg
      901 gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa cggacagtac
      961 ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg ctgctcattt
     1021 caacttatat gttttagtga actaccccca cttagcttgg ataggctaca attaactaga
     1081 cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt ttatgtctaa
     1141 gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc tttacttagc
     1201 tggaaagcca cgtgtagaaa tcattcgaga atatgaacta ac
//
LOCUS       DARDHD010000310         1204 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000310 DARDHD010000000
VERSION     DARDHD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.318"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            13..486
                     /locus_tag="SDA06_002845"
     CDS             13..486
                     /locus_tag="SDA06_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG4230045.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            495..722
                     /locus_tag="SDA06_002846"
     CDS             495..722
                     /locus_tag="SDA06_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4230046.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          379 a          193 c          206 g          426 t
ORIGIN      
        1 agagggataa aaatgaaatt taaaacattg ctattaaaat ttgtcgtttt aggtataagt
       61 gccgtattta ttttgttcgg cgtacttttt tatactcaga tcgctacaag cgaaaaagga
      121 ttcaattttg attggcagac cacattattt gtttctgtca tttactttgc ccttctccta
      181 gggatcgtta ttgcttattt cttattgaaa ctattgaaaa aaatcgaaga taaccaagct
      241 ttttccactc aaagcttaaa catactaaaa aaaatcgaac gaaccatttt gtttattggt
      301 ttcgcctttt ttggcttact tcctaaattt atgaaagtag cggatttaga cgatgctcca
      361 ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt ttgcagtata cacattaaca
      421 agcgtgctgg aaaaactgct tgaacatgcg attttactta agacagatca tgatttgaca
      481 gtgtgaggtg cgacatggca attatcgtta atttagatgt agtattagct caaaaaaaaa
      541 tgagtgtgac tgaattgagt gagaaagtgg gcattacgat ggcaaacatc tctattctaa
      601 aaaatggaaa agcaaaagcc atccgttttt ccactctcga aaaaatctgc caagttttgg
      661 agtgtcaacc aggtgatttg ttgacctata aaaatgactc gaatactata ccagaagaat
      721 aaaataaagt agaacaaaca gtaaataaaa aataatctga atgatagatg tgtgagactt
      781 ttagaagtta tttacgtcta ttattcagat tttttatttc gattgaatac cgttttatct
      841 ataaataaga tatgtactga ctcatacaaa gggggccttt tcttctccat gtactatcac
      901 ttttcctgat acaactggtt tcctgttacg ttgtagttgg atctgctttt agtataatac
      961 tttattcatt ccaccagact gatagcagtt tgagcagtct aaataatcat gtagtattac
     1021 aggccattct gtttcatacc ttgaagtgct taaaacttca gctaatggcc cattgtgtta
     1081 actcttactc cattcttttt tagtaagctc ccatatatac tgtaaagcct tctatatatt
     1141 atgtcatgtt gctttttaac atttactatg tagttttgat aaaatgattt gaataacagt
     1201 gatg
//
LOCUS       DARDHD010000311         1190 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000311 DARDHD010000000
VERSION     DARDHD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.320"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(406..780)
                     /locus_tag="SDA06_002847"
     CDS             complement(406..780)
                     /locus_tag="SDA06_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4230047.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..>1190)
                     /locus_tag="SDA06_002848"
     CDS             complement(803..>1190)
                     /locus_tag="SDA06_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4975 domain-containing protein"
                     /protein_id="HEG4230048.1"
                     /translation="YFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTF
                     GKTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGT
                     ALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          409 a          220 c          145 g          416 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa
//
LOCUS       DARDHD010000312         1183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000312 DARDHD010000000
VERSION     DARDHD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.321"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SDA06_002849"
     CDS             <1..153
                     /locus_tag="SDA06_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG4230049.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SDA06_002850"
     CDS             159..575
                     /locus_tag="SDA06_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230050.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SDA06_002851"
     CDS             681..944
                     /locus_tag="SDA06_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4230051.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="SDA06_002852"
     CDS             944..>1183
                     /locus_tag="SDA06_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4230052.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DARDHD010000313         1180 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000313 DARDHD010000000
VERSION     DARDHD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.322"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(139..1065)
                     /gene="cysK"
                     /locus_tag="SDA06_002853"
     CDS             complement(139..1065)
                     /gene="cysK"
                     /locus_tag="SDA06_002853"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG4230053.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          318 a          278 c          184 g          400 t
ORIGIN      
        1 atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca aataattttt
       61 ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat aaacctatct
      121 ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat aacgttcacc
      181 attgtcggga accacagcta ataccttctt gccttttcct aattcttttg cgacttttag
      241 cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt ttcgcccaac
      301 ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat catacacaga
      361 agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt gaggtcccgc
      421 ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt cgattttcgg
      481 atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac cgatacctgc
      541 aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac cagtcgtctt
      601 ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt agccgttttc
      661 ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac caggagtcaa
      721 aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca tcgtctcagg
      781 catcacgatg attactttat atccttttgc aactcctacc atcgacaatc cgattcctgt
      841 atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat cgtgttctgc
      901 tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat taaaaaactc
      961 aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt tgacgattgg
     1021 cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca attcctcctt
     1081 gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag tgattcgtct
     1141 ttgactagca aaaaaaagac aaagatatac catatatggt
//
LOCUS       DARDHD010000314         1167 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000314 DARDHD010000000
VERSION     DARDHD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.323"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..1145
                     /locus_tag="SDA06_002854"
     CDS             6..1145
                     /locus_tag="SDA06_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG4230054.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEAN
                     NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTK
                     RKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          421 a          171 c          202 g          373 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gtttaccaag caagttcaaa agaagaagca aataatcaaa
      841 taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga
      901 atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa
      961 ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat
     1021 ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa
     1081 agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt
     1141 tttaacttga tcaacagaat gggcttt
//
LOCUS       DARDHD010000315         1159 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000315 DARDHD010000000
VERSION     DARDHD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.324"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1159
                     /locus_tag="SDA06_002855"
     CDS             <1..>1159
                     /locus_tag="SDA06_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG4230055.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          381 a          233 c          225 g          320 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaa
//
LOCUS       DARDHD010000316         1153 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000316 DARDHD010000000
VERSION     DARDHD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.325"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..493)
                     /locus_tag="SDA06_002856"
     CDS             complement(<1..493)
                     /locus_tag="SDA06_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4230056.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEI"
     gene            complement(592..942)
                     /locus_tag="SDA06_002857"
     CDS             complement(592..942)
                     /locus_tag="SDA06_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG4230057.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          414 a          219 c          184 g          336 t
ORIGIN      
        1 taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca
       61 aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga atactactat
      121 aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga
      181 tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat
      241 agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac
      301 ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat
      361 aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa
      421 tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa
      481 tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta
      541 ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct
      601 gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg
      661 aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt
      721 aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac
      781 ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat
      841 ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat acataggata
      901 aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt
      961 ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca
     1021 aaaaaacaaa ggttctataa tattttgtgt tggtggaaaa tcgaaggcga ttttctacta
     1081 acactttttc ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca
     1141 aaacgtcatc cta
//
LOCUS       DARDHD010000317         1151 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000317 DARDHD010000000
VERSION     DARDHD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.326"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1022)
                     /locus_tag="SDA06_002858"
     CDS             complement(<1..1022)
                     /locus_tag="SDA06_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4230058.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRY"
BASE COUNT          356 a          230 c          185 g          380 t
ORIGIN      
        1 atatatctta ctttatctat ccgactattt agacgacggg tctggcaaac aggttcgcca
       61 gtggtaacct gatatccttt tagctctgct aaacaaacac taagcccatt tgtaaaaaaa
      121 gttaaatcat tgcgataatc ttgaatacat cgagcaggaa tttctccaat aataatgacc
      181 tcattatttt tcagttgagt atttacgata tttgcacaat atttgggagc atcgttatat
      241 gcccgtgaaa gatattcctg tggtgcataa actttaaaac taagatatgg ctctaacaat
      301 tctgttccag cttttctaaa ggcttgctcc agtacaatag gagtaagcat ccgaaaatct
      361 gctggagtac taacagggct atagtataaa ccgtacttaa aacagatttt acaatccgtc
      421 acattccaac catataatcc ttgttcgcaa ccatagcgta tcccttccat aactgcattt
      481 tgaaatgatt gatttaagta tccaagagaa accgagctct catactgcat tccacttccc
      541 aacggaagcg gtgatacaga taaaccaatg gaagcccaga aaggatttgg cggcacttcg
      601 atgtgaatgg tatattctgc attttttaac ggtctctcca tataaatgac tgtaggctct
      661 tttagttcta tctccacatg atacttttct tgcaacagtg cactaatcac ttccatttgt
      721 actttcccta agaaagaaag tataatttca tgtgtcgtag aatccacgta atatcgtaga
      781 agcggatcac tatctgagat ttccaaaagg gcatcaagca acatttctct ctgttcaggt
      841 ttactcggtt caacagttgt ttgtagtaga gggtgcggat tttcaatctt ttttctctgt
      901 ggcaatagtt ttgtatctcc aagaacacta tttaacttca aaaactcatt ttgcaaaata
      961 acaatttctc cagaataagc tctatcaatc ttacataatt caccatttat tgaagtatac
     1021 atttctgtaa cttttatttt ttctttttct gatactctaa ccgaatctcg taaatgtagt
     1081 actccactat aaaggcgtat atatgcaagt aagtagttca gttttggagt acaaaatgac
     1141 aaaatgtact t
//
LOCUS       DARDHD010000318         1127 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000318 DARDHD010000000
VERSION     DARDHD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.328"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            67..1107
                     /locus_tag="SDA06_002859"
     CDS             67..1107
                     /locus_tag="SDA06_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4230059.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          464 a          168 c          191 g          304 t
ORIGIN      
        1 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
       61 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      121 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      181 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      241 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      301 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      361 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      421 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      481 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      541 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa
      601 tcagagggta tgaaaatcat accagaactt aataataata tattagacac taatagacac
      661 aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca
      721 attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa
      781 acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag
      841 aaaaaagctg aaatagaaag tgatatttcg atagtttttg aagaactcga tagttattat
      901 gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc
      961 gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa
     1021 aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgcaag ctccccaagc
     1081 actattacga aagactggtt agagtgaaac tttactgaca aagtgat
//
LOCUS       DARDHD010000319         1124 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000319 DARDHD010000000
VERSION     DARDHD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.329"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..830
                     /locus_tag="SDA06_002860"
     CDS             <1..830
                     /locus_tag="SDA06_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4230060.1"
                     /translation="IVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNV
                     LRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKE
                     LDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAV
                     ELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISK
                     DTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            863..>1124
                     /locus_tag="SDA06_002861"
     CDS             863..>1124
                     /locus_tag="SDA06_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4230061.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKY"
BASE COUNT          436 a          124 c          210 g          354 t
ORIGIN      
        1 ctatagttca caatcaggtt tattttggcg ggggaattaa tttatggtta gccaaaaaga
       61 atggaatcag acagagaatt gctcatagcc atgcaactga agatggaaaa tctcaaaata
      121 ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt gttacaaaat gcaacagact
      181 accttgccgt tagtcaagaa gctggtgaaa gtctgtttca aaaccatccc tttgagatag
      241 ttcataatgg tattgattta gagctttata gtaaaaattc agaagctaag gttaataaga
      301 gaaaagaatt ggatatctca atgagtactt ttgttgtggg aaacattgga cgattagaga
      361 aacaaaaaaa tcaatcttat ttgttagaga tttttttaga aatactaaaa atgaattctg
      421 attcttactt gattttagta ggtgaagggt cacttagaaa taaactgaaa gccaaagcag
      481 tagaattgga tatagagaaa agagtaaaat ttttaggaga aagaaacgat attcccgagt
      541 tattatctgt gatggatgtt tttgtaatgc cttctcttta tgaaggtctg ccaatggttg
      601 gattagaagc acaagcagca aaattaaaat tagtcttagc agatactatt tccaaagata
      661 caaagttaac aaataatgtg gattttgtat cattagatga aacaccacaa gaatgggcaa
      721 aaaaagtaat aaaagagccg tatgctaata aaataacaca agaactaaag agttatgatg
      781 tttcgtacac tactgaacaa atggagagta tatatagtag agatcattaa aaatgttcaa
      841 gatattttag gagtgattat ttttgaaaat atgttatttg atattggcat acaatgatat
      901 tggcaactta aagagattgg tcaatcgatt gaacgaaaat gcagttttta ttattcatat
      961 agataaaaaa actgatatag aaccatttat tgaagcattt aaatcatatg ataatgttga
     1021 atttttatgt aataaaaacc gtgttaaaat ttattggggt ggtttttcca ttgtacaagc
     1081 agaaatcaat ttagtaaaga gagcattaca aaatgagaaa tatt
//
LOCUS       DARDHD010000320         1117 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000320 DARDHD010000000
VERSION     DARDHD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.330"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SDA06_002862"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SDA06_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG4230062.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          160 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaatg
//
LOCUS       DARDHD010000321         1114 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000321 DARDHD010000000
VERSION     DARDHD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.331"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..75
                     /locus_tag="SDA06_002863"
     CDS             <1..75
                     /locus_tag="SDA06_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4230063.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            80..1027
                     /locus_tag="SDA06_002864"
     CDS             80..1027
                     /locus_tag="SDA06_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG4230064.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          351 t
ORIGIN      
        1 gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat
       61 ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg
      121 ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc
      181 aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt
      241 cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca
      301 tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt
      361 gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa
      421 acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa
      481 agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca
      541 atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa
      601 tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt
      661 agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg
      721 aagccagtct agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc
      781 gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact
      841 tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa
      901 agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat
      961 tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat
     1021 caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt caataaggac
     1081 tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DARDHD010000322         1105 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000322 DARDHD010000000
VERSION     DARDHD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.332"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SDA06_002865"
     CDS             <1..1087
                     /locus_tag="SDA06_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4230065.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DARDHD010000323         1102 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000323 DARDHD010000000
VERSION     DARDHD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.333"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(24..1082)
                     /locus_tag="SDA06_002866"
     CDS             complement(24..1082)
                     /locus_tag="SDA06_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG4230066.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          428 a          186 c          132 g          356 t
ORIGIN      
        1 aagcaatgaa agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt
       61 cccaaataat accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc
      121 tccaccattt catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca
      181 taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa
      241 gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta
      301 tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt
      361 ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa
      421 gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg
      481 gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat
      541 tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa
      601 tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc
      661 atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca
      721 aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa
      781 gaaagtgtaa tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt
      841 tttgttattt ttgaaattaa tacactaatt aaacttaaaa taatagaagt atatccaaac
      901 atagctccaa catttggact tgtatagaca ccaaataacc gatgttcaat aaaaccttgt
      961 cttgcaagta atcctggcgt tcctgttggg acatgataag aaatcaaaaa aataaattga
     1021 tagatagata ttagcgcagc taaaaaaatt atgataatga aaacattatt aaaatacacc
     1081 atttttttct ttttttcctc tt
//
LOCUS       DARDHD010000324         1100 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000324 DARDHD010000000
VERSION     DARDHD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.334"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            163..903
                     /locus_tag="SDA06_002867"
     CDS             163..903
                     /locus_tag="SDA06_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230067.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          406 a          193 c          208 g          293 t
ORIGIN      
        1 cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat atatttatga
       61 aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg
      121 ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga aatcagacga
      181 aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac caacgacaag
      241 aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca gtccccgttt
      301 gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt
      361 cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa gcataaagga
      421 aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg acgaaaaaaa
      481 ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga gtccaagcat
      541 actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt tgttcctaaa
      601 tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga ggatcattta
      661 atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga accgacgccg
      721 tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta
      781 gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc
      841 tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag ctattttaaa
      901 taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc ggcgtttcct
      961 tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga gtttaagcga
     1021 attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac tattttcgca
     1081 gatcgtttgc aaaaatagtt
//
LOCUS       DARDHD010000325         1091 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000325 DARDHD010000000
VERSION     DARDHD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.335"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..481
                     /locus_tag="SDA06_002868"
     CDS             <1..481
                     /locus_tag="SDA06_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG4230068.1"
                     /translation="TLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRN
                     EDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKY
                     WRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            537..755
                     /locus_tag="SDA06_002869"
     CDS             537..755
                     /locus_tag="SDA06_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230069.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            826..>1091
                     /locus_tag="SDA06_002870"
     CDS             826..>1091
                     /locus_tag="SDA06_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230070.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFF"
BASE COUNT          414 a          132 c          208 g          337 t
ORIGIN      
        1 tactcttaaa gtgaagtctt taagaattca accatttatg cttagtggta ggggaatgca
       61 aaatagggaa atagtgccaa ctgtttctca atatagaaaa ctagtttctg atattaacta
      121 tattaaaaga aatgaagatt ataccaagat agattgggga gatccaattg atcatatcat
      181 ccgacacaga actttattga aagatttttc gaattttgtt tctattaggt cagacggagg
      241 attaatggca tcaccttact tgccaattca agttggaaat attaaatgtc acagtctttc
      301 taaatattgg cgagcaggac tttataaagt tttttcttta gatttagttc aagatatagc
      361 tactcaagtt tcttcaatac aagatatgag taaagaatta aaaaaaggac atattaaatg
      421 gcttaatgaa gatatagaaa ttgatattct cgatgaatac aaacatgata ttttaattta
      481 ggtttatcat tggaaagttt agataaacaa agtactttta gaaaggaggg aaaaaaatga
      541 tttctgaacc tcaaatgaat aatatgtatg aaagcccaac tataatggga gctggtgaag
      601 ctgcgccaag agcttgttca gcaattatgg tgtgtgtgtt attagcatct gcaggagtag
      661 gtatccaagc aggagcggtg tatacttatg ctgtagttgc tacagtagct gtagctgcag
      721 ccgcagtttg gaaaacagta ggattagttg gttaggaatt aataggagac taactgtctc
      781 ctattgattc caatgattaa gctaaggagg aattttaaga aaataatgaa atacaataat
      841 ttaaaaccga aattacttaa tgagaacata gaatatataa gagaagaaaa ttatggatat
      901 ttaaccatag ttcccaaaaa tggaactgaa ttgaaagata acgttttcaa tccaacagta
      961 agatatatat tggctaactg tactggaaaa aacagtgtaa aaaaaatagc gaatggttta
     1021 aaaaagaagt ttcctagttt agaatatgaa aaattctatc aagatgttgt aggaactatt
     1081 aaatttttca a
//
LOCUS       DARDHD010000326         1087 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000326 DARDHD010000000
VERSION     DARDHD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.336"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            64..849
                     /locus_tag="SDA06_002871"
     CDS             64..849
                     /locus_tag="SDA06_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG4230071.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            842..1084
                     /locus_tag="SDA06_002872"
     CDS             842..1084
                     /locus_tag="SDA06_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230072.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          427 a          161 c          177 g          322 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
       61 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
      121 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
      181 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
      241 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
      301 aataaataca tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc
      361 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
      421 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
      481 agtattttag aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc
      541 ggctctgtat atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa
      601 gtgaaaaata aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca
      661 attagaaaat ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc
      721 cccaattgga aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta
      781 actgaaaata gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc
      841 gatgactaac aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa
      901 acaaaaaaac tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg
      961 gatttacgaa acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat
     1021 tactaaactt aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt
     1081 ttaaaag
//
LOCUS       DARDHD010000327         1075 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000327 DARDHD010000000
VERSION     DARDHD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.337"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..412
                     /locus_tag="SDA06_002873"
     CDS             56..412
                     /locus_tag="SDA06_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4230073.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            402..668
                     /locus_tag="SDA06_002874"
     CDS             402..668
                     /locus_tag="SDA06_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4230074.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            827..>1075
                     /locus_tag="SDA06_002875"
     CDS             827..>1075
                     /locus_tag="SDA06_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4230075.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGH"
BASE COUNT          443 a          136 c          208 g          288 t
ORIGIN      
        1 tcgaattttt atgttatata ctataatata aaatcaatat atgatataat tgggtatgga
       61 aaaaatatct tttgaaacat ttaaaagacc taatggtcat gatgaatttt tagaatggtt
      121 ggaaacatta ccaaaaaaag atagtgcgaa gcttttacgt acaatagaag aaacagaaaa
      181 gaatgggatg ctagtagctc aacggttgaa atgggtgaaa aaattagaca ctaatttgta
      241 cgaattaaga agtaaggtag gatcaaacat ccaaagagct atctatttcc atgtggtcaa
      301 tggaaggtat gtcattaccc atggatttac taaaaaaacg gagaaaacac caataagtga
      361 acttaaacat gcaaaagaac ttagagagga gtggttcaca aatgatgatt aaaaaattga
      421 ttgaagaaaa gcaaaaagac aaagattttg cagaagcata tgaccgtgaa ggaagaaaac
      481 tcgaaactgc ggttgctctt tatcatgcac gggaggaagc aggcctaact caagcggagc
      541 ttgcacaaaa agcacataca actcaatcta ctattgctcg aattgagaga ggggataacg
      601 tatctttcga taaacttgat gatatcgcta gggcattagg caaaaagctg gtagtagaat
      661 ttgcttaaaa gcgagttgaa aatatacctg taataggctc ctaactaatc aattagttta
      721 gtgggccttt ttgtactaaa tagataaaag ttgtcctata gtgtaaaagt caataaattt
      781 tatacaaaaa ttactttaaa gagacataaa gagaaaggta caataaatga aaaccataaa
      841 tctaataaaa gtatttatag catcaccagg tgatatagca gcaaagagga atgaagtaga
      901 agaaaccata tatagatgga atatagaaaa tacagataat acaaaagtaa ttttgatgcc
      961 aattcgatgg gaaacgaatg cgattcctac ttatataaat aatagctctg ggcaaagtgt
     1021 tattaataaa caaattgtag aaaatagtga tatattatta gcgatttttg gccat
//
LOCUS       DARDHD010000328         1072 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000328 DARDHD010000000
VERSION     DARDHD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.338"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            326..988
                     /locus_tag="SDA06_002876"
     CDS             326..988
                     /locus_tag="SDA06_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4230076.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT          367 a          143 c          215 g          347 t
ORIGIN      
        1 ggtgcacata attgaaacct ccttatgtaa ctgaatatga ttgtatcatc ttttcatttt
       61 agaataaacc tttatatgta attttgcact tgacacttca gatggacgtt ttcttcaaaa
      121 taatatgttt tgaaacttga taagagaagc cactgtcttt actaaaaagt aaaggctgcg
      181 ataaaggaca gtgactttaa taaagttgca ctgttgtatt gtctttggat aaaattgtct
      241 aaagggaagt tttatcccca acgaaaaaac atctaaagtg gtaagtaatg aaagaatgtc
      301 gataaagtag gaatggggga tctttttgaa tacaatagaa tcacagtttg ataaggttgc
      361 agaagattac gatttcgtga atgagctttt gaatgattat tcattctttg tgtctaatat
      421 gtctccaaag aaaggcagag cattagatat cggatgtggc tcgggtttgt tagtggagaa
      481 attagcaagt tattatgatg aagtggtagg gattgatatt tctaatcaaa tgctcgatct
      541 tgccaaatct aaacgtcaac taacaaatac ggtctatctg aatatgaatg cagaacaact
      601 taattttaat gagaagtttg attttattgt aagccgaaca acctttcatc atttggatga
      661 tatagccagc gtaatacagc aaatgaagga actgttgaat gaggaaggaa gaatagttat
      721 tcttgataat gtatctgaag tcgagacacc gcctacctat gtttataaat taggggcaat
      781 ccaagagttt ttaccgcatt gtttcaaatt tggaataaaa aatgcgatta gaatttataa
      841 tcacaacaca tctaaatcat ggcttgagca tttagcttca gataagtacc tatctgaaca
      901 aaattattac gatttatatg aaaagttgtt gcctggatgc caattccata aaatgggttg
      961 ggcaatgggg gttgtctgga caaaataact taaagctgtg gctttgaaaa gttgaatttg
     1021 tgattgtttt cagagctatc gggtgcttta gtataaataa caatgaataa aa
//
LOCUS       DARDHD010000329         1064 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000329 DARDHD010000000
VERSION     DARDHD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.339"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..390
                     /gene="spx"
                     /locus_tag="SDA06_002877"
     CDS             <1..390
                     /gene="spx"
                     /locus_tag="SDA06_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4230077.1"
                     /translation="PSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEG
                     GVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDE
                     IRSFVPREIRCVARNLIYLQNNNILNS"
     gene            404..760
                     /locus_tag="SDA06_002878"
     CDS             404..760
                     /locus_tag="SDA06_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG4230078.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          368 a          161 c          204 g          331 t
ORIGIN      
        1 cctagctgta cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt
       61 gaagagaaga atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca
      121 gcgactgaag gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg
      181 ggcatagatt ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct
      241 tcattattga gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa
      301 gatgagatcc ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat
      361 ttacaaaaca ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta
      421 tggatcagcg cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt
      481 gccttatcaa tttgttgact tagaagaacg cgatctaagt acagaagaag cagaagaatt
      541 atgcagtttt gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca
      601 caacattgcg attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca
      661 acataccaat ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata
      721 caatgagcag ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa
      781 gtcactgagc atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg
      841 ttcttttttt gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt
      901 caaatctagg tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat
      961 attcatttct gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa
     1021 atagttgttg acatgttaga aatgacatga taagataact gagt
//
LOCUS       DARDHD010000330         1062 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000330 DARDHD010000000
VERSION     DARDHD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.340"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            243..>1062
                     /locus_tag="SDA06_002879"
     CDS             243..>1062
                     /locus_tag="SDA06_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4230079.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          372 a          152 c          181 g          357 t
ORIGIN      
        1 attcttttat tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct
       61 agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc
      121 tacacagaca aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa
      181 aagaaagtcc attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc
      241 tcatgcccaa ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg
      301 aattatttcg atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag
      361 cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg
      421 ggaattattc acgttcattc agaacagagt atggagaatt aaatttggta attcctagag
      481 atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt
      541 tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat
      601 tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa
      661 tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca
      721 tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt
      781 atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc
      841 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa
      901 aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg
      961 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
     1021 aggttcgtgt tagtgatcga caagaaatct gtagcgattt ca
//
LOCUS       DARDHD010000331         1047 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000331 DARDHD010000000
VERSION     DARDHD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.341"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..603
                     /locus_tag="SDA06_002880"
     CDS             <1..603
                     /locus_tag="SDA06_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4230080.1"
                     /translation="HSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQV
                     VYYNQRTFLDKDGKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVT
                     VQSVSKDYGDDFYKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRD
                     YFSETIQPGKSAQGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            719..>1047
                     /locus_tag="SDA06_002881"
     CDS             719..>1047
                     /locus_tag="SDA06_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4230081.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETY"
BASE COUNT          420 a          170 c          170 g          287 t
ORIGIN      
        1 cactctgaat taattgaaca tggaaatagt gcaaaagata taaaaaaatt agagatgctc
       61 caatatactt attcaactga ttctgatgat gaaacaatgt taatttgggt tagtccaaaa
      121 actgaacagg ttgtatatta taatcaacga actttcttag ataaagatgg aaaatccaaa
      181 gattcaagct ctactacaac tacaactaca acttcgtctt cagaacccca gacggcatca
      241 gttgcacata gtataggaga agcagttcct tttacttctg aaaatggtaa ctctattaac
      301 gtaactgtcc agtctgtatc aaaagattac ggtgatgatt tctataaacc agaaggccta
      361 ttttacgcaa aggtagattt tcttgtagaa aacacaggga ctacaccttt tgatgcaagt
      421 tcaagttatt tagaatttta tgattcatca gatattaaat ctgatttagc tagtagagat
      481 tacttttcgg aaaccatcca accgggtaaa tccgcacaag gaacagcata ttttgaagtg
      541 aaaaatgatg gaacaacatt tgaagtgttc ttcgcagata cttcatggaa aggtagctac
      601 taaacaaaaa aacacgcccc ctccctcgcc aaagtttgtg gacgtgatga aaaataaacc
      661 tgctataatt ggcttactta tccattccta ttatagcaac aaataggaga tgaaaacaat
      721 gtggattgaa accaaaactg ataaaaacgg aaaaaaagta tataaatata atgagcgata
      781 tattgatcca aaaactagaa aaagaaaaaa agtgagcatt acttataaaa ataaatctcg
      841 agaaactcag aaagtggcat tgctagagtt aaataaaaaa attgatataa aattaaacga
      901 aaaaacactt cagaagcctg atctaacatt tcatgagctt gtcgaagaat ggttagttat
      961 ttacaaaaga caagttaagg agtctacata ttatcctacg aataatatct taaacactat
     1021 aaagaaaaag atacctgaga cttacat
//
LOCUS       DARDHD010000332         1025 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000332 DARDHD010000000
VERSION     DARDHD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.342"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            116..730
                     /locus_tag="SDA06_002882"
                     /pseudo
     CDS             116..730
                     /locus_tag="SDA06_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          376 a          162 c          173 g          314 t
ORIGIN      
        1 cgtttgtcta tttagagttt accctaaata gacaaaaata taacattgaa aaatttagag
       61 ttctaacgta tgattagggt gtattataaa cgttatagaa aagtagggaa taaatttgaa
      121 aattgcttat attagagttt catccatcga tcaaaatgaa caacgtcaaa ttgaagaaat
      181 gaaaaagttt ggcgctgaaa aaatttttat cgaaaaacaa tctggagcga cgattagtca
      241 tcggccgatt ttccaggaag ctttagattt tgtcagagag caagatattt ttattgtaga
      301 agccattgat cgacttggac gcaattatga cgagattatc gattcagtga attatctaaa
      361 gaagaagaat gtgcgactta ttatcaccag tttaccgatc atggctgaag caattggaaa
      421 tcctctccta gataaattca tcaaagactt aatcatttaa atctcagcca tgattgctga
      481 acaagaacgt actgaatcga aacgacgcca ggcacaagga attaaaatcg ctaaagcaaa
      541 cggcgtctat aaaggtcgtc ctaagttata cagtgcagac accaaagatc ctcaacgtcg
      601 ccttgtctat aggagtatcg ttcaggatct tgagaatggg gttgctatct caaagattgc
      661 aacagactac aatgtgacta ggcaaacaat ttaccgaata aaaaaagaaa tcgaccaact
      721 aattgtatga tttctttttt gaatcacgta attattaatt agctttgtca tcaatacaga
      781 caatcatttt agattcatct agctaagttg attacattta tgttaaaaag aatttagtta
      841 acataaatat aattgatgca taaatcgtcc ttatttaaag gcatttaaca ttgacaactt
      901 agtataatgt atcttatgta aactaataag atttgaattt tattttatac acactcgata
      961 atagatcaat tttatggtcc cccgcattta cgattgcaca agagaaacat aaagtcgacc
     1021 tatta
//
LOCUS       DARDHD010000333         1012 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000333 DARDHD010000000
VERSION     DARDHD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.344"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SDA06_002883"
     CDS             <1..704
                     /locus_tag="SDA06_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4230082.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          341 a          163 c          169 g          339 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa at
//
LOCUS       DARDHD010000334          995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000334 DARDHD010000000
VERSION     DARDHD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.345"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SDA06_002884"
     CDS             complement(26..913)
                     /locus_tag="SDA06_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG4230083.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DARDHD010000335          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000335 DARDHD010000000
VERSION     DARDHD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.346"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SDA06_002885"
     rRNA            complement(<1..>994)
                     /locus_tag="SDA06_002885"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARDHD010000336          972 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000336 DARDHD010000000
VERSION     DARDHD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.347"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SDA06_002886"
     CDS             complement(14..706)
                     /locus_tag="SDA06_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4230084.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="SDA06_002887"
                     /pseudo
     CDS             791..967
                     /locus_tag="SDA06_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          289 a          184 c          171 g          328 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg ct
//
LOCUS       DARDHD010000337          961 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000337 DARDHD010000000
VERSION     DARDHD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.348"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..902)
                     /locus_tag="SDA06_002888"
     CDS             complement(<1..902)
                     /locus_tag="SDA06_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4230085.1"
                     /translation="MKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFKYNQQKPKDC
                     FSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYALDRKVI
                     HNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYLFLDEVH
                     NLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLATFRRRIPAE
                     ITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFEGNVGELRNE
                     IKLLCAEGYLGNKRKSSIYLGDKL"
BASE COUNT          311 a          173 c          134 g          343 t
ORIGIN      
        1 tctagtttgt cccctaaata aattgaagac tttctcttat ttcctaaata accttctgca
       61 caaagaagct tgatttcatt cctcaattct ccaacatttc cttcaaatgt tttcgataaa
      121 agatcagtaa aaagttcaat cgaacaaaat atttttgaat caatcgtaat tgcttcatct
      181 ttaagaaatt caaataataa ttgaattttt tcagatgtac ttctattatt gtaatctggc
      241 agagtgattt cagctgggat gcgtcttcta aaagttgcta acaaagaggt atttttatct
      301 tctgttgtgg ctaagataag gcgaacattt gcatactccc attgcacact ttcaccaagt
      361 ttacgatact tttgagaatc cataagaaga aacaatttct cttggttttc tgcacttaga
      421 ttgtgaacct catctaaaaa aaggtatcct ccattagcag aagatagcat ccctgcagta
      481 tctttttcag ccccagtaaa ggctcctttt ttatatccaa ataaaactgc tgacaatagc
      541 tctggattat ttgcataatc tgcacaattc acaacaacaa attttgcgtc attatgaatt
      601 acttttctat caagagcata ctgatagatc aaacttgcta aaaaactttt tccaacgcca
      661 ctgtttcctt ttattattaa tgggaggcca ttagggggat acattacagt agctttgcat
      721 ttatctatag tggatttcaa agatatgtct gctccaataa acttagaaaa gcagtctttt
      781 ggcttttgtt gattatactt gaacattact ggtctaacag aatcattctt tagtaatttt
      841 ccatctgaaa ataactgatt caaatagtgg gaagcgacat ttcttttaat acaaaatttc
      901 attgcaatat cttgagcaga aaaatcgtaa tactttaaat tatttttgat atactctaat
      961 a
//
LOCUS       DARDHD010000338          960 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000338 DARDHD010000000
VERSION     DARDHD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.349"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..278
                     /locus_tag="SDA06_002889"
     CDS             18..278
                     /locus_tag="SDA06_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230086.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(640..879)
                     /locus_tag="SDA06_002890"
     CDS             complement(640..879)
                     /locus_tag="SDA06_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230087.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          342 a          141 c          179 g          298 t
ORIGIN      
        1 ttaaggatgg atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa tatatttgcc
       61 aacctattgg cttgaaacaa aaagtggttg gagaagtaat cagtaaatta gaaaattgtg
      121 ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta gaaaaatgcg
      181 gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa tatctatata
      241 actatagcaa caaatatgaa catactttag tttcataaag atttaaataa agtgccttca
      301 aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat tgttcatgat
      361 ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg cctggtggag
      421 aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac aaactagtag
      481 ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata tcaggatcat
      541 ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta ggagtaaacg
      601 cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag tttgtttgaa
      661 ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag aaacttttat
      721 atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat cgatactatt
      781 cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag cagtaagtaa
      841 caataaattt gataaactga taaacagaat tcttttcatt atgtacacac tccttatagt
      901 atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac tatcaagtct
//
LOCUS       DARDHD010000339          959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000339 DARDHD010000000
VERSION     DARDHD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.350"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SDA06_002891"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SDA06_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="SDA06_002892"
     CDS             complement(570..>959)
                     /locus_tag="SDA06_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230088.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DARDHD010000340          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000340 DARDHD010000000
VERSION     DARDHD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.352"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SDA06_002893"
     CDS             <1..>931
                     /locus_tag="SDA06_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4230089.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARDHD010000341          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000341 DARDHD010000000
VERSION     DARDHD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.353"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(178..726)
                     /gene="aac(6')"
                     /locus_tag="SDA06_002894"
     CDS             complement(178..726)
                     /gene="aac(6')"
                     /locus_tag="SDA06_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4230090.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          267 a          200 c          139 g          325 t
ORIGIN      
        1 cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctct ggtacacggt
       61 gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca agaagtaatt
      121 ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct ctttttatta
      181 ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt tgtcccagcc
      241 atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga attcatacgg
      301 atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat acaggtccgt
      361 ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg tgattcctcc
      421 tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt ggttctttcg
      481 tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt attgagggat
      541 tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga ttcgttctgg
      601 attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg gccaagtcag
      661 tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa attcactgat
      721 tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa aaaacgaaat
      781 gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat tatttatctt
      841 acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa gatcaggaaa
      901 gccatcaatt ggaggaaaaa actctgtttg t
//
LOCUS       DARDHD010000342          927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000342 DARDHD010000000
VERSION     DARDHD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.354"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..788
                     /locus_tag="SDA06_002895"
     CDS             <1..788
                     /locus_tag="SDA06_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230091.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHK
                     DGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          341 a          146 c          168 g          272 t
ORIGIN      
        1 ttgctttgat tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta
       61 agcttcatag acaaccaaaa gggattatca aatccgctac aatatcacgt cattctagcg
      121 gtaaatacta tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt
      181 ccacaattgg tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa
      241 ttgataataa taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat
      301 tgtctaggcg tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt
      361 atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg
      421 attttctgaa taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag
      481 acttaaacac aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt
      541 cttggtcaaa gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca
      601 tcaaagtaga taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg
      661 gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc
      721 gtgatatcaa tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat
      781 tggcttagat ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag
      841 acaccgctgt tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctcccac
      901 ttcaagcgtt aagaaccgtc aggttta
//
LOCUS       DARDHD010000343          917 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000343 DARDHD010000000
VERSION     DARDHD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.355"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            14..>917
                     /locus_tag="SDA06_002896"
     CDS             14..>917
                     /locus_tag="SDA06_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230092.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKL"
BASE COUNT          298 a          118 c          131 g          370 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattag
//
LOCUS       DARDHD010000344          916 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000344 DARDHD010000000
VERSION     DARDHD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.356"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..355
                     /locus_tag="SDA06_002897"
     CDS             86..355
                     /locus_tag="SDA06_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4230093.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="SDA06_002898"
     CDS             348..605
                     /locus_tag="SDA06_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4230094.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          352 a           99 c          174 g          291 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataat
//
LOCUS       DARDHD010000345          907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000345 DARDHD010000000
VERSION     DARDHD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.357"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..907
                     /locus_tag="SDA06_002899"
                     /pseudo
     CDS             274..907
                     /locus_tag="SDA06_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          302 a          172 c          170 g          263 t
ORIGIN      
        1 tgtaatcatc aggaaaaaca atcttaaacg aattactatt catggtctta gacacacaca
       61 tgctagcttg cttttagcaa gctagtgcaa atattaaaga tgtgcaagag cgtttaggcc
      121 actcttctat tcaaatcatt atggatctat atattcatat tacagacaaa agaaaagaag
      181 aaaccgtagc gcaattcgca aaatataggc tctataattt atgggactga tgaatcaaaa
      241 ttgattcttc agtcccattg ttattttagg tatatgattc gtacattgaa agagttgaaa
      301 aactatcttc cgaagccgtc aaaaaggacc ctgccaaaag tattgatggt ggatgaattt
      361 cgttcgcacg cttcaattgt ggataaaatg agctttattt gtgcggatgg cgagactgga
      421 aaactcattg atattttgcc tacacgaaaa ttgtctcgat taacacacta ttttagtcgc
      481 tgtgcaaatc ctgaagcggt ggaatattta gtgacagata tgaacgccgc ctacttccaa
      541 ctgacaaaac gtgtcttacc aaatgcgaaa gtcgtcattg attgttttca tatcatcaaa
      601 catttgaatc aggcgttcaa tgattttcgt gtacaagaaa tgaaaagatt acaccagtta
      661 gggcaacgac gggaagccga gaaagtgaaa aagaactggc gctttcttct aaaaaaccgt
      721 caaaaaatca atcactatga ttacaaaaca tggcgcagtt ttcgtgcacc taaatacccg
      781 ctactcactg aagcaatgat gattgatcga ttgctggcat tttcaacgcc tttaaaggac
      841 gcctatactt ttttccatga gctgaccgaa gcctttagag acaaggaatc agacttattt
      901 tttacct
//
LOCUS       DARDHD010000346          903 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000346 DARDHD010000000
VERSION     DARDHD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.358"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            263..886
                     /locus_tag="SDA06_002900"
     CDS             263..886
                     /locus_tag="SDA06_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4230095.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          274 t
ORIGIN      
        1 gttttaaaat aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac
       61 ttaaaggagg tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt
      121 tgttgtttct tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt
      181 tttcagcggt ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata
      241 gattggatag gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca
      301 aagtttagct cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga
      361 agacgattta aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga
      421 gctgcaagag cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga
      481 tcgattagca agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg
      541 tgcttctatt gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag
      601 agacttgcta acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa
      661 aaaaatcaaa gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac
      721 tggtaaaaag attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga
      781 aattgttgat ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt
      841 tacaagaaaa accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag
      901 aaa
//
LOCUS       DARDHD010000347          890 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000347 DARDHD010000000
VERSION     DARDHD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.359"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..888
                     /locus_tag="SDA06_002901"
     CDS             <1..888
                     /locus_tag="SDA06_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG4230096.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DARDHD010000348          875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000348 DARDHD010000000
VERSION     DARDHD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.360"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..464)
                     /locus_tag="SDA06_002902"
     CDS             complement(243..464)
                     /locus_tag="SDA06_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230097.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="SDA06_002903"
     CDS             complement(562..807)
                     /locus_tag="SDA06_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230098.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          264 a          168 c          112 g          331 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatc
//
LOCUS       DARDHD010000349          872 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000349 DARDHD010000000
VERSION     DARDHD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.361"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..>872
                     /locus_tag="SDA06_002904"
     CDS             94..>872
                     /locus_tag="SDA06_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4230099.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DARDHD010000350          867 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000350 DARDHD010000000
VERSION     DARDHD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.362"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            36..>867
                     /locus_tag="SDA06_002905"
     CDS             36..>867
                     /locus_tag="SDA06_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4230100.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          299 a          151 c          167 g          250 t
ORIGIN      
        1 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
       61 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
      121 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
      181 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
      241 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
      301 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      361 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
      421 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
      481 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
      541 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
      601 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
      661 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
      721 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
      781 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
      841 tgaaatctat ggatttcaat tcagtag
//
LOCUS       DARDHD010000351          835 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000351 DARDHD010000000
VERSION     DARDHD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.363"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            428..>835
                     /locus_tag="SDA06_002906"
     CDS             428..>835
                     /locus_tag="SDA06_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4230101.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT          288 a          154 c          125 g          268 t
ORIGIN      
        1 acttctaact gttctttatt aattaaaaat gttctgtaat aaaataaaac tttataacaa
       61 ctataacaaa accgaatgca aaacagaagt tctataagtt ataatcatag ttgttaggtg
      121 attcatctag taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc
      181 aaccttcttc aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa
      241 acaggaagcc aaatggctaa gacatttttt ctattagtct agttgtttct cgtcgcaagt
      301 ttaagacttt agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca
      361 cagctcctta cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag
      421 tttttttatg cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg
      481 gctgaaagac catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac
      541 aaggaaagag atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc
      601 taaacgctcg attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga
      661 gcttgtcgat ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg
      721 aaagaaatta ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg
      781 gcgcagaagt caactgaaat ctgtctataa cgattttaca tgggtaagca atcta
//
LOCUS       DARDHD010000352          830 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000352 DARDHD010000000
VERSION     DARDHD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.364"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..309
                     /locus_tag="SDA06_002907"
     CDS             <1..309
                     /locus_tag="SDA06_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HEG4230102.1"
                     /translation="ETLEDAFEMAQEVLEGYLVLLELQEKEIPSPTPVQELNARIKRS
                     ENVNLFVVKANTKVAKARNKQNYDRKSVTVEHYLNVLAAEENINLSKALNDLLREKLI
                     "
     gene            529..>830
                     /gene="tnpA"
                     /locus_tag="SDA06_002908"
     CDS             529..>830
                     /gene="tnpA"
                     /locus_tag="SDA06_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG4230103.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          288 a          126 c          164 g          252 t
ORIGIN      
        1 gaaactttag aagatgcttt tgagatggcg caagaagtat tggaaggata cttggtgctt
       61 ttagagttgc aagaaaaaga aattccatcg cctacgcctg ttcaagagtt aaatgcaaga
      121 atcaagcgat ctgagaatgt taatttattt gtcgtaaaag ctaatacgaa agttgctaaa
      181 gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg tggaacacta tttaaatgtt
      241 ttagcggctg aagagaatat taatctgtcg aaagctttga atgatttgtt gagagaaaag
      301 ttgatttgat gtctctcaga tgatcatatt aagcaaaaac agcttgtttt aggacgtatg
      361 gatttaacag aattaggcag aagtcgccca tcctctatag gtgggtgatg aatgccgttc
      421 ggtatgaggg ttaccgttgg taactcgaag accgacaact aaggcgtatt gcctttttat
      481 tttgtcccat gttacaatca gtctatacaa gaaatgagtt gatataacat ggaattagat
      541 acaaataatc attcagtatt tcttctttat taccaccttg ttttagtaac gaaatatcgt
      601 agacaagtga ttgatgatga aatatctgat tatgccaaaa ctacttttga aagaatttca
      661 gaatcgtatc acattacttt agtggagtgg aatcatgata aagaccacgt tcatattatg
      721 ttcaaagctc aacctaaaac agaattgaca aaattcatca atgcctataa aagtgccagc
      781 tcaagattga taaaacgtga ctttccaaga gtcaaacaat ttctttggaa
//
LOCUS       DARDHD010000353          827 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000353 DARDHD010000000
VERSION     DARDHD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.366"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            111..695
                     /locus_tag="SDA06_002909"
     CDS             111..695
                     /locus_tag="SDA06_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4230104.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          269 a          115 c          173 g          270 t
ORIGIN      
        1 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
       61 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
      121 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
      181 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
      241 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
      301 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
      361 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
      421 agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac
      481 gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa
      541 aattgtctga acaagatttg gaattactga agactttagt ggatagcggt acacctatta
      601 agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa
      661 tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt
      721 tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt gtctactgat
      781 aacttattct caaatgttgt aaggatttta ttcatttgtt aatatta
//
LOCUS       DARDHD010000354          816 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000354 DARDHD010000000
VERSION     DARDHD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.367"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            11..199
                     /locus_tag="SDA06_002910"
     CDS             11..199
                     /locus_tag="SDA06_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG4230105.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(257..610)
                     /locus_tag="SDA06_002911"
     CDS             complement(257..610)
                     /locus_tag="SDA06_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4230106.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          265 a          142 c          122 g          287 t
ORIGIN      
        1 ggagattgtt attataaact acaagtggat attgtgtcct atttgtggaa ataaaacaag
       61 actacgaata cgagtggata ctatacttaa aaatttccct ttatactgcc ccaaatgtaa
      121 gaacgaaact ttaattaatg ttcaaaaaat gaatataata acaatcaaag agccagacgc
      181 caagacgcag agccgataat ttgagaaatg aaactctcat cttatcggct ctttttgttt
      241 atctgaattt tactgactag ccttcaatat ttcttgcttc attgatacct tttgctagtg
      301 cttccattaa ggatagttct ttgtctgtaa agctatccat gtatttttct acctgtaacc
      361 ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa ttcatcaata gatacatgga
      421 gtaatgatac aagatcatac agcacttgtg tacttgggtg ttgtcctttg ttttctatat
      481 ttgttaaata acgtgggtca atttcaatca ttgttcccac ttgttcacga gtgagacccc
      541 gtttcattcg agcctcttta atcgctaaac caacggctct aaaatcgtat ttatcttctt
      601 ttttacgcat actaaatcac ctctatacat tttactgttc ctatataacc ataaacaggt
      661 atagaaaaac atgttatata gtttatgagt tcctatagaa cactgtggaa tttattaatg
      721 cgatgaatcc aatttcaaat agaaaggtcg ctgattttat ttacctatac aactttataa
      781 aatttagtca tactcctaga aagtagataa aattgg
//
LOCUS       DARDHD010000355          804 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000355 DARDHD010000000
VERSION     DARDHD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.368"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..799
                     /locus_tag="SDA06_002912"
     CDS             86..799
                     /locus_tag="SDA06_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4230107.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          280 a          105 c          168 g          251 t
ORIGIN      
        1 tgtgtgtata attttgatgt ggattttatc ggtttattta tgttaaagta tagatgaaga
       61 tatgtttttg attggagggt caccattgaa aaaaattgcg aagtatatgg aaatttactt
      121 agatataaag aaaaaaattg taaatggaga gtataaaatt ggtgagaaac ttcctacagg
      181 agatgaattg gcagttattt atcatacaag caagttaaca gtaaagaaag gcgtagactt
      241 acttgtttcc gaaggagttt tgcgaagtag aagtgggtat gggactgaag tcgtcagaac
      301 accaattgat aactcacgag ttcttggtcc aaacgaaggt ttgtttagcg tggttggtga
      361 agaacacgta gattcagaaa tccatacttt ctcaattgaa cttccatcga aaaaagtagc
      421 tgataaatta agtatcagtg taaaagacta tgtatataat attgtacgaa gccgttttat
      481 tgattgtcaa ccatactcga ttgagcaaac attcatgcca ttagcagtca taccaggctt
      541 ggaaccaaaa catttaaaga aatctgtata tgcttatatc acaaatgaac tgggtctgga
      601 aataaagact tcgcacatac gtatcaaagg agatactgcg aatgaatttg acgcaaaaat
      661 attaaaaata gatccaggtg aatttatgat tgagatagac aaagttgttg ctctatcgtc
      721 tggtaaacct ttcgagtatt cggtatcgag acatttatat caagactttg tttttgaagc
      781 tgtttttgtt gaaaaataag ctcg
//
LOCUS       DARDHD010000356          801 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000356 DARDHD010000000
VERSION     DARDHD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.369"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..587
                     /locus_tag="SDA06_002913"
     CDS             <1..587
                     /locus_tag="SDA06_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG4230108.1"
                     /translation="NGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSR
                     DALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRI
                     TVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLG
                     FSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            604..>801
                     /locus_tag="SDA06_002914"
     CDS             604..>801
                     /locus_tag="SDA06_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230109.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARES"
BASE COUNT          289 a           90 c          150 g          272 t
ORIGIN      
        1 gtaatggaat aattccaagt gttagtggag atacgattcg gtctgaaaaa aattattcta
       61 tcattgtttt tgaaaagtta gcacaaacgt ctataacatt aggaatggat ataatagaag
      121 cgtatcaaag tagagatgct ttgatacaag aaaacgaatt agctgtatcg ttacctgagg
      181 tcttaaaagt gagggattct gggattgttt attatactaa ggaaatcgga aaaactaaga
      241 tagagcatct ttctccctta atttcttctg tcgttcaatt catcggctta aatatctata
      301 aaagaattac tgtaaaggaa atagctaatt atttttctgt aagtgaaact aaattaagag
      361 aaagctttaa gaatgaaatg catattacga tttataatta tatctctaag cgaaaaattt
      421 ctacggctaa aataatgttg aaatcaaacc atactatcag tgaagtttct ttaggtttag
      481 gattttcaga ttcttcacac ttttcgagag tattcaaaaa gtacgcaggt gtatctccta
      541 agcaatatca attaggttta gtggacaatt tcaacaacat tagctgagag gttttatttt
      601 attatgagac tatatcattt acgtagtttg gaggagttga ggaaattgga ttttaacaaa
      661 aaattttttt gggaaatttc tgatttgagt tgtttcgtag aatctgagcg tctaagtaat
      721 ggtaataata gtgtgttaaa tgaatatgac aaagtagtaa ttaataaagt aaaaagttta
      781 tgtgaagaag ctagagaatc t
//
LOCUS       DARDHD010000357          797 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000357 DARDHD010000000
VERSION     DARDHD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.370"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>797
                     /locus_tag="SDA06_002915"
     CDS             <1..>797
                     /locus_tag="SDA06_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG4230110.1"
                     /translation="LPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRL
                     TSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIR
                     EMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDR
                     LLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEG
                     ITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          268 a          159 c          151 g          219 t
ORIGIN      
        1 ctccccagag tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg
       61 agctttattt gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa
      121 ttgcctcgat taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta
      181 gtcacagaca tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa
      241 gtagtgattg atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt
      301 atccgtgaaa tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa
      361 aagaactggc gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca
      421 tggaaaagtt ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga
      481 ttgcttggct tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa
      541 gcctttcgag acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg
      601 ttagatgacg gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc
      661 aacgcgatga tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag
      721 acaatgaaac gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt
      781 ttgatcaatc aattaat
//
LOCUS       DARDHD010000358          796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000358 DARDHD010000000
VERSION     DARDHD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.371"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            85..318
                     /locus_tag="SDA06_002916"
     CDS             85..318
                     /locus_tag="SDA06_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG4230111.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            315..722
                     /locus_tag="SDA06_002917"
     CDS             315..722
                     /locus_tag="SDA06_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4230112.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          298 a          112 c          149 g          237 t
ORIGIN      
        1 agttagacat gagagggact atgcttgatt taaaatcaac aagcgtatat actgtatata
       61 caaagaaaaa ggagggagaa gactatggca attaaagaaa gaaaatttag ggaagtgggg
      121 aactcagtag tattaactat ttcaaaggag tttcttaaag aaaatgggtt aaatcccgga
      181 gatacggttt ttttagatga agaaaaactt cgagcggcaa ttacaaaaaa acaagcctta
      241 tctgatgaaa ttgatttagc aatatctcaa gcattaactg aatatgatac tacttttaga
      301 gagcttgtcg ataaatgagt attcattacc tagaagccaa agatattata aaaatgaatg
      361 taatccaaat aaaaaaatac tctcctaatg aaccccttgg tgtaaaggat gcgaatgctt
      421 tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg tgaagaggtt tatccttcga
      481 tttatgaaaa agcagctatc ctacttatac aattgataaa gaaacatcct tttcacaatg
      541 caaataaacg tactgctttc ttagctactt ttgtcttttt gaaaatcaat ggacagcttt
      601 tagctattga tcaaaaagat gctgtaaatt tagcggtata tattgctacg tatgataagg
      661 atttcgatca actaaaatat gaagtgatca acactataaa gacacatacg cagcccattt
      721 aaggagttac tgtatgtgtc tttatagggt tattagctgc tatagcgaaa taattgaagt
      781 aaattacaaa tagcta
//
LOCUS       DARDHD010000359          772 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000359 DARDHD010000000
VERSION     DARDHD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.372"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..>772)
                     /locus_tag="SDA06_002918"
     CDS             complement(109..>772)
                     /locus_tag="SDA06_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG4230113.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          213 a          178 c          149 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gc
//
LOCUS       DARDHD010000360          763 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000360 DARDHD010000000
VERSION     DARDHD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.373"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            19..>763
                     /locus_tag="SDA06_002919"
     CDS             19..>763
                     /locus_tag="SDA06_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230114.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHP"
BASE COUNT          292 a          111 c          130 g          230 t
ORIGIN      
        1 gaaagtgagt ggtaattaat gaaaatgaat cacacttcaa ttcagaagca tttacaactg
       61 ttaaatacac tggggatttc ttatgttaat ttgagaactt tttctacatt aaacaagaaa
      121 accgtttctc atattttttc tactgcagat tctcaagcaa ttattaacta tttagaaaaa
      181 cagaaattgt ctgaaggtac agtaaacata acttataatc ctattaaaca agaggtgatt
      241 acacaaaaaa agcattctgt gagagacaat gatattgaaa aaattcggtt tgtatttatt
      301 gatattgacc ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa aaaggcagaa
      361 aatgttatgg aacaagtaga acgttatttg aaagaaaaga gaatagaatc atttgtaaaa
      421 gttgatagtg ggaatggcta ccatattttt cttcccataa atgaacaacc aaataatcac
      481 gagacgattc ttactattca gaattttctt caattacttc accgaagatt tggagtagaa
      541 gatgaagttg atattgatac ttctgtttat aatccttcaa gattatgcaa attagttggt
      601 acaccagcgg tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc tagtttcctt
      661 tctattcccg ataacataga tagaagtaat tgttttaaaa ttcttgaaca gatagttgaa
      721 gaaaattcca tgatgtcaac ctctagagta aaaagccatc cat
//
LOCUS       DARDHD010000361          752 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000361 DARDHD010000000
VERSION     DARDHD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.375"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..417)
                     /locus_tag="SDA06_002920"
     CDS             complement(<1..417)
                     /locus_tag="SDA06_002920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230115.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEI
                     QYEGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKK"
BASE COUNT          234 a          134 c          109 g          275 t
ORIGIN      
        1 ttttttgatt ttatcatctg cacaaaggag attttttaaa tctaatcctt cttttatttc
       61 aaaagttgaa ttttttaaat ctataagagt aaaaccaatt ccttcatact gaatttctga
      121 aaatcgaatt tttctcccag ttgatagatc aaggggcaga aaacctttta ttacattgtg
      181 agtgtcacca tcatttacaa tgtcattagg aattttgatt ttagaattct gtggagcacc
      241 tattggaaga taataattca ttataatttt gtagtcttca tgaggaaata aagtactttt
      301 aggattgcta agatattctc gtaccgaatc taagaatggc ttatccattg aatcttcatc
      361 cccatttaaa ctaaaaaaca ttcctacaat tgcttttgca actctaagtg aattcatttt
      421 ctttattgaa tgtgttaaat cagacttacc attctttaaa cttgtattgt tataaaggaa
      481 atttttattg tcttctgcaa atctaatata ttcctcaaca tactcactac caaaaaaact
      541 attacattta ctacataacg tattggaatg gcaacagttt ttttgacaaa ttttataagg
      601 tgcagaactt ctttccgtat gctattccga ttgtccttga caatgagcct cctcggatgt
      661 gacgatcttc ggcaggagct aacagttaaa gttagactgc ctcgctagcg ttagcacatc
      721 cgagctcatt atcaaggatt cgctgcgcca at
//
LOCUS       DARDHD010000362          746 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000362 DARDHD010000000
VERSION     DARDHD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.376"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            194..424
                     /locus_tag="SDA06_002921"
     CDS             194..424
                     /locus_tag="SDA06_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230116.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            430..729
                     /locus_tag="SDA06_002922"
     CDS             430..729
                     /locus_tag="SDA06_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230117.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          286 a          114 c          113 g          233 t
ORIGIN      
        1 acaaaaccac tatattctat tatgtatcta ataccctcaa aacagctata aatcaacaat
       61 accgacatat aataaacact atgttcaatc tgagatatat caaagataaa cgtatttttg
      121 cccccttttt gcccctcaaa tatcgcttta caacgagaac gtaagttcgt ataatcgttt
      181 tgaggtgatt tttatgatgg aagactttat tagaaaaaat atcagtgatg aatatgcaga
      241 cttttatgag caaagcagca aaaaagataa attccagatg gatgtttcaa ttttagctat
      301 attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag tattctctga
      361 aggcaaaata aaaactcgat atatattaga ggtagaaact aattttaaaa acagatcgga
      421 gtaatggtta tgctttttaa tgagacacaa ttatggttta aattcgatcc tacgaataga
      481 tttatcaaag atttttataa agtatggaat tcagaagttt tctttttagc aatcgaagat
      541 agcttattaa tcaatctcta ctattctaat aagaactatt tcaaaattcc tgctgcgaaa
      601 actagaatga agaagggtgt atactttttg tttgatatcg tgactgacgt gccggacgca
      661 cgtagcgatc atcggcgtta tgactatata aagtatactt tcgttgatcc agaaagatac
      721 aaagattaaa taaaaaaata acaaac
//
LOCUS       DARDHD010000363          744 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000363 DARDHD010000000
VERSION     DARDHD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.377"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..536)
                     /locus_tag="SDA06_002923"
     CDS             complement(<1..536)
                     /locus_tag="SDA06_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230118.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLT"
BASE COUNT          296 a          119 c          125 g          204 t
ORIGIN      
        1 gtcagcatat cactaggagc cataccactt aattcttttc ccagtgggac tttcaattct
       61 tgtgctctag attgttgaat aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg
      121 acggtagctt ttaaaagact taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa
      181 agaaacaacg ttcctccaat aaatacagaa accatagcga cagaaagtaa atgagtatac
      241 actactagcg tcatgccaat agacaaccag taccattttt ggtaatctct aatataaatt
      301 tcgtaacacc ccattagtat caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa
      361 gcacgatgga atatgtcaat ggcacgatag gcggaaaatg tataaataat cgaaaataac
      421 agcgaaatat aacgattgtt ttttatttgt ttcatagaaa aataagaaac gaccattgtt
      481 aaaaaagtga taaaaaaata gtagatatta taacttaaaa caagatttcc catcatttta
      541 taaaataaaa aagctggata aagagtaagc catggataaa aaatattcat cattgttcca
      601 tgatgatcga agttattgaa gtttactgga gaactccata cattatctag tcctatgatt
      661 cgagataaat gaaagtaagt atcctgattg acactatgat agattccctt agacatgaat
      721 agagggatga cgtaagcaaa actt
//
LOCUS       DARDHD010000364          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000364 DARDHD010000000
VERSION     DARDHD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.379"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SDA06_002924"
     rRNA            <1..>733
                     /locus_tag="SDA06_002924"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARDHD010000365          732 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000365 DARDHD010000000
VERSION     DARDHD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.380"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..520)
                     /locus_tag="SDA06_002925"
     CDS             complement(<1..520)
                     /locus_tag="SDA06_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4230119.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLS"
BASE COUNT          259 a          114 c          102 g          257 t
ORIGIN      
        1 tacttaaatc gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag
       61 tagaaaacat tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt
      121 gctcaaaggt actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat
      181 tctcgaataa cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat
      241 tgacaaattt cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt
      301 tttcgaatat acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat
      361 acttgaaaat agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt
      421 tttgtgaaag gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc
      481 ctttattcaa gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc
      541 ataaagtcat attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt
      601 cgcctttttc gataacgata gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag
      661 agacacaaat taggcgtaat tctaataaaa atcttagaat aaaaattttt actgatttaa
      721 tttagaatgt tt
//
LOCUS       DARDHD010000366          725 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000366 DARDHD010000000
VERSION     DARDHD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.381"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..621)
                     /locus_tag="SDA06_002926"
     CDS             complement(<1..621)
                     /locus_tag="SDA06_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4230120.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSF"
BASE COUNT          247 a          139 c          100 g          239 t
ORIGIN      
        1 aaacgaggta atcgtaataa tgattgggat taatacatta aaagacttta aaacagcggg
       61 aggaatattt ccggatagct gaatctgtag tttcttatta ttagaaagtt taataaataa
      121 ttctgtagcc gctaatccaa caaatatacc tacaaacatt cctgaagctc ctgtatagga
      181 tataggtatc acacccgtca cttcaacagc tttatccgca ttttcaggag taaacatcat
      241 ctgcataggt gttaatacaa caaatgtcga aatagcaact aataccgctg caatatgatt
      301 tgtataattt ctgcttgcac acatatgata agatactgct gctacaacta ataatgtagt
      361 tatgcttaat gtaccattgg caattgaagt accaatttgt tgtaacgtgg tcaatgtact
      421 agtatcgaca atttccccca tgaatcctgt tggttctaag atcactgaat taataagtgt
      481 cacaaatcct gctaacatca taaatggcat aaatgtagca aacgcatctc gcaaagttct
      541 taaatggatc tgattcccca acttagtcgc aactatacca aatttttcaa atacaatgtc
      601 cttcctgcta cttttgccca tattttttct cctttatgct tattgtaatc gattactttt
      661 tccacccaaa taatacatca cttatcaaaa aaagtaaata cttttaatta aatatatata
      721 tcttt
//
LOCUS       DARDHD010000367          719 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000367 DARDHD010000000
VERSION     DARDHD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.382"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..>719
                     /locus_tag="SDA06_002927"
     CDS             44..>719
                     /locus_tag="SDA06_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4230121.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVN"
BASE COUNT          339 a           83 c          111 g          186 t
ORIGIN      
        1 ttaataactt atttaaggaa attttttgaa ggagaagata aaagtgagaa aaaagattat
       61 tattaatgct ggagtaatta taggattgat gttagtaatt ttctccggaa agcaatatgc
      121 tgataatatt ttattatggg gaggggaaga taatattgaa aagatcaaca acaatttaac
      181 tatattagac acagctctag aaaataaaga gcaaaaaatt agtattttaa attcacaatt
      241 gtcttcaaac aaccaaactc ttgaacaact aagaactaat attgaagttt ccaaacaaaa
      301 agtggcatct ttagaaaacg acaagaatca attagtatca gagaaaacta atttagaaaa
      361 tcaattgtca gcaaaagact ctgaattgca aggaaaacaa aatgaaatta atgcgaaaat
      421 agacgaaatt aaccaaaaag tagctgaaat aaatcgatta aatagcgatt ggaataatcg
      481 tttacaaaat gaaaagaaca aatttaatga agaacaatct actattaata gtttaaacca
      541 gcaaattaat caaatgttga gtgaaaaaaa taacattcaa aatcaactgc aagctactca
      601 gcaaaagcta gaagaagaaa agtcaaaaaa tagtagtttg aataattaca ttgaaaagct
      661 tcaaaaagct aaaaatgatg tagaggatac cgcaaataaa gcacaacaaa ttgtaaatg
//
LOCUS       DARDHD010000368          713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000368 DARDHD010000000
VERSION     DARDHD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.383"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>713
                     /locus_tag="SDA06_002928"
     CDS             <1..>713
                     /locus_tag="SDA06_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HEG4230122.1"
                     /translation="SREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLA
                     LILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEII
                     LLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQ
                     NVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNEN
                     YLSELRKENNILFIVGTLN"
BASE COUNT          261 a           90 c          129 g          233 t
ORIGIN      
        1 tagtcgagaa gttcaattag tcaatcgttt tattcaaata ggggatattt atttaacaaa
       61 aattcaaaaa aatgttttgg ttattttatt taagttaatg tgtagtaaag aatttcagca
      121 aaaacgttta gcactgattt tggctcatgg agatctaacg gcaacctgta tagcaagtac
      181 tgctaatagg atcgttgaga gatatatata cgacgccatt gatatgccac taatgacttc
      241 aagtagtgag attgcagaca aaattaatag atacttacaa gagtgtaccg gatatgaaga
      301 aattatttta cttgttgata tgggttcact ggagaaaatt tatctaaatt tggaactaga
      361 cgctcaaaaa aaattcggac tactaaacaa tgtttcaaca agaatggcta ttgatgttgc
      421 aaatcagata ttattagatt taccgcttaa acagattctt gaacaagtaa aagaaaatca
      481 aaatgtggat tatatttatg aatcatcaaa tattaagaag gatatgattg tctgttcttg
      541 tgcaagcgga ttaggaactt cgtttaaatt aaaaaagata cttgaagata gctttccaag
      601 ggatactgat cttgtcatag aaacgtgtga ctattactct ttagtgaatg aaaactattt
      661 atcggagctt cgtaaagaga acaatattct ttttattgtt ggaacactaa atc
//
LOCUS       DARDHD010000369          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000369 DARDHD010000000
VERSION     DARDHD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.384"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SDA06_002929"
     CDS             complement(<1..446)
                     /locus_tag="SDA06_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4230123.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARDHD010000370          704 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000370 DARDHD010000000
VERSION     DARDHD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.385"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          298 a          106 c          122 g          178 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgt
//
LOCUS       DARDHD010000371          696 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000371 DARDHD010000000
VERSION     DARDHD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.386"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..448)
                     /locus_tag="SDA06_002930"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="SDA06_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          209 a          140 c          105 g          242 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctat
//
LOCUS       DARDHD010000372          673 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000372 DARDHD010000000
VERSION     DARDHD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.387"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            82..450
                     /locus_tag="SDA06_002931"
     CDS             82..450
                     /locus_tag="SDA06_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG4230124.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          235 a          114 c          102 g          222 t
ORIGIN      
        1 cttgtgtttt gttcgttttt tatacataac tattgttttt ctggtaaaat aaatcaaacc
       61 acttaatgga ggcgttttag aatggaaaaa tttcagccga tcatcaatca aatcaaagaa
      121 aaagaacatc aacaaaagat caaagacctt tttgctaaga taacagaaac gtatccccag
      181 ctagacacaa ctgtaaaatg gaaccaacct atgtttactg accatggtac ctttattatt
      241 gcatttagtt ttgctaaaaa tcatttttct atcgcaccgg aaaaagcagc gattcgtgcc
      301 cttgaaaaaa atatccaaga agcaggctac gtatatacgg ataacgtcat caaagttcct
      361 tggaaaagtg taatcaactg ggaattgatt gaacaattga tttcatttaa tattgaagat
      421 aaaaaaggtc atgataaatt ttggcgataa agctttatac gaataaacct tttactggcg
      481 aggaaactat tcatcacctt atacaggttt tgttcaatcc tatccttttt atcatccctg
      541 gttaaacact taattgacaa agtatctgct atttttataa ggagtctttc ttgtgctgaa
      601 aaaaatcact gattgaatgg ctttgaattt tctacatagt aaaattattt tttcagcaat
      661 tttcctgcaa aac
//
LOCUS       DARDHD010000373          665 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000373 DARDHD010000000
VERSION     DARDHD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.388"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..613)
                     /locus_tag="SDA06_002932"
     CDS             complement(<1..613)
                     /locus_tag="SDA06_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230125.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          191 a          134 c          128 g          212 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaa
//
LOCUS       DARDHD010000374          652 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000374 DARDHD010000000
VERSION     DARDHD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.389"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>652
                     /locus_tag="SDA06_002933"
     CDS             <1..>652
                     /locus_tag="SDA06_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG4230126.1"
                     /translation="IDLSLSHDFKINEVQYTQVSEMYHTMLEKNKEIDFDMILLVAYK
                     ILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIY
                     GSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKN
                     DRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKE"
BASE COUNT          244 a           88 c          113 g          207 t
ORIGIN      
        1 aattgatttg tcactttctc atgattttaa aattaatgaa gttcagtata ctcaagtttc
       61 tgaaatgtat cacactatgt tggaaaaaaa taaagaaatt gattttgata tgattttact
      121 agtagcgtat aaaattttaa ctgtaaacag taacgttgca cgtaatattg ctagtaatat
      181 aagggcgatt tatgtggatg aatttcaaga tactagagaa ttacaataca atgtaatagc
      241 taaattactt caaaacaacc ctcaaattca gtcaatgttt gttggagata ttgaccaagc
      301 aatttatgga tcattagatg gtatagctaa atcagttgaa gaactagaaa tgctaactag
      361 tcacactttt caatcaaaga cgctacatgg ctgttacagg tctaatcaga gattagtaga
      421 cttctactct aattttcaga gttgtcctta cgagattgaa agcagaggga ataataaaaa
      481 cgaccgtggt tttataagtt atgattgcaa agttactaag gaggatttac ccaacataat
      541 aaaaaatata ataaaggaaa agattgaaag cggcattcat gaaaaagata tttgtgtact
      601 tgctcctcaa cactatcctc tgttttcact gggtgagtat ttaaagaaag aa
//
LOCUS       DARDHD010000375          643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000375 DARDHD010000000
VERSION     DARDHD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.390"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..180)
                     /locus_tag="SDA06_002934"
     CDS             complement(<1..180)
                     /locus_tag="SDA06_002934"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4230127.1"
                     /translation="METRDYIITHQKLIESGLSHFEKEGFQQTKIRQLCLTAGVTTGA
                     FYKHFTSKEALFEEII"
     gene            complement(472..>643)
                     /locus_tag="SDA06_002935"
     CDS             complement(472..>643)
                     /locus_tag="SDA06_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG4230128.1"
                     /translation="RKKKLKNNDLAKVLSVSPASVSEMLAKLRYEDYLDIGFQLTDKG
                     KKFIDDYKKGFK"
BASE COUNT          212 a          122 c           73 g          236 t
ORIGIN      
        1 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
       61 tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa
      121 atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat
      181 gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc
      241 aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga
      301 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
      361 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
      421 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
      481 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
      541 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
      601 tagaactttt gctaaatcat tattttttaa tttctttttt ctt
//
LOCUS       DARDHD010000376          641 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000376 DARDHD010000000
VERSION     DARDHD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.391"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            269..>641
                     /locus_tag="SDA06_002936"
     CDS             269..>641
                     /locus_tag="SDA06_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4230129.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQ"
BASE COUNT          242 a          100 c           92 g          207 t
ORIGIN      
        1 aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac atgtttgata
       61 tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca ttatcaacta
      121 aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag
      181 ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct
      241 ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca
      301 gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca
      361 agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt ttactaaaga
      421 tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg ttgaagatat
      481 aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa
      541 atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta caagacaggg
      601 tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa a
//
LOCUS       DARDHD010000377          639 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000377 DARDHD010000000
VERSION     DARDHD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.392"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..259)
                     /locus_tag="SDA06_002937"
     CDS             complement(<1..259)
                     /locus_tag="SDA06_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4230130.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIA"
     gene            complement(277..597)
                     /locus_tag="SDA06_002938"
     CDS             complement(277..597)
                     /locus_tag="SDA06_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4230131.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          201 a          125 c           88 g          225 t
ORIGIN      
        1 tagctatttt atctgcttct gtttcaaatt ttgagtgaaa agttgtagaa gtatcgtaca
       61 aaactgaata ttcaaaatga gaagcggtga aatgcgcaag ttcatgagat aaatgaaagg
      121 cttctgcagt ttcgccatat agattttcat tcaaaaaaat gattctaggc ttcggataat
      181 agaagccagg ctctttcatt tccatgtaaa ctactttcaa attgtattcg ctcaacattt
      241 ctttcaattt caaatacata caacccatca ctccaactat tcattttcct ctaaagcttt
      301 agcaattgca atcgctttac gcattgtctc cttagatatt tcttttccat caaaagaaaa
      361 aacagtatcg ttttctgata aatccacatg tttaggggtt tctctttctt ctctacctag
      421 aaggtagtct acagagactc caaagtaatc agctaaccga gctaactcaa tagaattagg
      481 agtttgtctt ttccaacttc ctaaatatcc attcgaatac ccgaaattca tttcgagctg
      541 tctaatggat aatccttttt cttttgctaa ctcctttatt atttcgtaag tattcattga
      601 taaatcaacc tttctgaatg cttacaaaaa aagtttaga
//
LOCUS       DARDHD010000378          636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000378 DARDHD010000000
VERSION     DARDHD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.393"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          216 a          109 c           83 g          228 t
ORIGIN      
        1 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
       61 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      121 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      181 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      241 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      301 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      361 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      421 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      481 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      541 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      601 ttaaatgttt cttacaatca aaaaaagctg ttaaat
//
LOCUS       DARDHD010000379          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000379 DARDHD010000000
VERSION     DARDHD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.394"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SDA06_002939"
     CDS             complement(<1..270)
                     /locus_tag="SDA06_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230132.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SDA06_002940"
     CDS             complement(276..>635)
                     /locus_tag="SDA06_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4230133.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARDHD010000380          608 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000380 DARDHD010000000
VERSION     DARDHD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.397"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(79..273)
                     /locus_tag="SDA06_002941"
     CDS             complement(79..273)
                     /locus_tag="SDA06_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230134.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(373..459)
                     /locus_tag="SDA06_002942"
                     /pseudo
     CDS             complement(373..459)
                     /locus_tag="SDA06_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          209 a          109 c           90 g          200 t
ORIGIN      
        1 aaaaatcaat acataaatta tcatatctgt atatgtcatt taggtatttc agttatgtta
       61 taatagtttt gctgtctgct accccggcct aatctggcaa ggggaggagg agatataatg
      121 aaataccttt ttgattttgt ttttgcaatc aagccacttg cagatgtagt gtgcagttac
      181 ctctgcaaat ggcttgattg caaaattgcc aaaaaagaca gtaagcctac ccgttaattc
      241 tataatatag aaacgtaaaa aaaaccccat cagcctgcac gctgatgggg tttggtgata
      301 taatgaaata cctctttgtt actcataatt gtaccataat tagtcacgaa tgcaaccaat
      361 agaaaaaact acttagaacg gtagatcatc gctaataaca atgcattgtc caccaaatgg
      421 attttgtgaa gcattaaata cattgttatt aattgtcgct gcttttaact aggcctctcc
      481 ccttctatta taaaagaaga tatatatcat ttcttaatta cctactacct taaaccaaaa
      541 tttaagtact acctaattta agtattactt aaacaatcta aaaaatagtt atcccaattg
      601 ttatgctt
//
LOCUS       DARDHD010000381          601 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000381 DARDHD010000000
VERSION     DARDHD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.398"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..>601
                     /locus_tag="SDA06_002943"
     CDS             39..>601
                     /locus_tag="SDA06_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230135.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITF"
BASE COUNT          217 a           86 c          115 g          183 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 t
//
LOCUS       DARDHD010000382          591 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000382 DARDHD010000000
VERSION     DARDHD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.400"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..>591
                     /locus_tag="SDA06_002944"
     CDS             26..>591
                     /locus_tag="SDA06_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG4230136.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DARDHD010000383          588 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000383 DARDHD010000000
VERSION     DARDHD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.401"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..150)
                     /locus_tag="SDA06_002945"
     tRNA            complement(65..150)
                     /locus_tag="SDA06_002945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="SDA06_002946"
     tRNA            complement(167..238)
                     /locus_tag="SDA06_002946"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="SDA06_002947"
     tRNA            complement(250..322)
                     /locus_tag="SDA06_002947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="SDA06_002948"
     tRNA            complement(340..421)
                     /locus_tag="SDA06_002948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="SDA06_002949"
     tRNA            complement(429..501)
                     /locus_tag="SDA06_002949"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="SDA06_002950"
     tRNA            complement(505..577)
                     /locus_tag="SDA06_002950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DARDHD010000384          573 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000384 DARDHD010000000
VERSION     DARDHD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.404"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            365..>573
                     /locus_tag="SDA06_002951"
     CDS             365..>573
                     /locus_tag="SDA06_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230137.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIA"
BASE COUNT          227 a           67 c          106 g          173 t
ORIGIN      
        1 aaatgggagt aaacgaaatg atgaaagtca gattgagttt ttaaatgata ccttcctcag
       61 aacttcaaaa cgaattcaaa gattcgttga caaatcttga gcaagatcat ttaaaaaaat
      121 attttctctt catttgacga aaaatgttta gaaatcttat ataagtgaag ataaagtaat
      181 tggtgtaatt aattgtagag aatagccttt gagcaattga agcgatacaa ttatttagaa
      241 aatgaacttc ctccagcata ctacatgatt ccttacggga cgatgttaaa gaagaaagca
      301 agaaatttta gaagtattag tagataaact aatgggcttt tctcaattta agggagtagc
      361 ccaaatgttt atcaaatatg gtgcagatac agtagcaacc atgtttacag aagaatatca
      421 agtagactat tatgaagtaa tagataaatg tgtacatttt gtttatgaaa aagtgtggaa
      481 ttttatgaat tttgcgataa gtgaagaaat cttacgagta caagaaaaca gaacaggaat
      541 atttaccaga gacagaaagc ttaatttgat tgc
//
LOCUS       DARDHD010000385          558 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000385 DARDHD010000000
VERSION     DARDHD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.405"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>558)
                     /locus_tag="SDA06_002952"
     CDS             complement(<1..>558)
                     /locus_tag="SDA06_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4230138.1"
                     /translation="QEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALD
                     DSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSK
                     NGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVS
                     GAYLTNEYQQTNIKDIYAIGDCATTF"
BASE COUNT          166 a          109 c           65 g          218 t
ORIGIN      
        1 aaaagttgta gcacaatccc caatagcata tatatctttt atatttgttt gctggtattc
       61 attagttaga tatgctccac taacatctct caagagttcc ttttccccaa ttggactatt
      121 agatagaaat cctatagctt gtacgaccat atccatagag tattgggtac cacttgtagt
      181 gactattttt tcaatctttt ttcttcctaa atatttttta ggactttctg atagacaaag
      241 atgtattcca ttttttgata gtatttcttc aactttatct gaaaaactac ggtcgtaata
      301 agtcgacaac actctatctg caacatcgaa aagatatact tctttccctc tttttcttat
      361 ggcttcagca atttcaactc caatatatcc tgctccaata attcctactt ttttaatagt
      421 actatcatcc aaagctttaa aaatctcttt tgcatttgga aaattcttta tcgaaaaaat
      481 attagctaaa ttattccccg gtatatctag cataatttgc tttgaacctg tagctaatat
      541 aactttgtca tattcttg
//
LOCUS       DARDHD010000386          555 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000386 DARDHD010000000
VERSION     DARDHD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.406"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>555
                     /locus_tag="SDA06_002953"
     CDS             <1..>555
                     /locus_tag="SDA06_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG4230139.1"
                     /translation="WPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSA
                     KGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTV
                     QRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILV
                     DKLMGLSEFKGISPIDAYCGLKDT"
BASE COUNT          205 a           70 c          110 g          170 t
ORIGIN      
        1 ttggcctatt gtaccagaaa aatataaaag gattttactg gtagatgtac atggggtaaa
       61 aggtgggttt ggtgaagtag ttgctggaaa tagacctcag gccattgatt tattgaaaca
      121 gattttgtca gcaaaaggat ttgaagttga aaaatacatt tcacctgaag aagaattgaa
      181 aggattatcg caagaagaaa aaattaaaaa tatagctgga atatatgctg ctaagagacc
      241 aatcaaagat ttaactgaaa aatatgactt gattattaat atagctaatg ttaacccagg
      301 tacagttcaa agaattgttt ggccagcaag taaaggtact ccggatattc cattttatat
      361 tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg tttcatttag ctgatgtgcc
      421 tcaagtaaaa acttatatca atacttatga tggaaaacaa gaaactttag aaatattagt
      481 ggacaaacta atgggccttt ctgaatttaa gggaataagt ccaatagatg cttattgtgg
      541 gttaaaggat acatt
//
LOCUS       DARDHD010000387          552 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000387 DARDHD010000000
VERSION     DARDHD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.407"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>552)
                     /locus_tag="SDA06_002954"
     CDS             complement(<1..>552)
                     /locus_tag="SDA06_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG4230140.1"
                     /translation="GLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILS
                     QIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSF
                     IVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLL
                     MCITCSSLIILTRPFSHILYAKD"
BASE COUNT          197 a          105 c           73 g          177 t
ORIGIN      
        1 agtctttcgc ataaagaata tgtgaaaacg gtctagttaa tatgataaga cttgaacagg
       61 ttatgcacat taacaaatta atcataacaa atgtatttcc ataaaatttt gcagtctcat
      121 tatctccata ttctttaatt gcagaaattt gccaggcctg tataaatatt ccctgcaaag
      181 tattaattat ttgaggtatt ttatatgcca cagacaataa gccattcgcc gagacaccta
      241 ctataaagga aaccacatac ctatcagctg tactattaat ccaccatcca acaacagttg
      301 caattaatgg aatcgagtaa tttagcattt ctctctttag ctgaggatct ttagcctcaa
      361 aatcgatata ttcccatatt ctcactaata ctgacagata agtagctgaa ataatctgtg
      421 ataaaatatt tgcaatgaaa aaaccaatta atccccattt cagaataagc aagaagaaaa
      481 tattggcaat tattgttaca attgtgctga taatacctgc tatgcccata gctttaactt
      541 tttctaaccc tt
//
LOCUS       DARDHD010000388          548 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000388 DARDHD010000000
VERSION     DARDHD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.408"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..493
                     /locus_tag="SDA06_002955"
     CDS             47..493
                     /locus_tag="SDA06_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4230141.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          190 a           83 c          117 g          158 t
ORIGIN      
        1 gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg aaaataagtt
       61 agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac ctcaacggta
      121 cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg agatttataa
      181 agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata accttcgctt
      241 atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta gccgatttga
      301 tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga ttgttgattt
      361 tcattaccca gggcttgaag atatagaaat ggctgcaagc agactgactg attttgagat
      421 taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt cagcaaaaaa
      481 ggcgcaaatg taaaaacagt ttctacaagt gttataccta aaaaaagcta gctgtgtaaa
      541 aaaaacgc
//
LOCUS       DARDHD010000389          547 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000389 DARDHD010000000
VERSION     DARDHD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.409"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          173 a          111 c           78 g          185 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaa
//
LOCUS       DARDHD010000390          545 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000390 DARDHD010000000
VERSION     DARDHD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.410"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            19..240
                     /locus_tag="SDA06_002956"
     CDS             19..240
                     /locus_tag="SDA06_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230142.1"
                     /translation="MHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIKPFLEIGDE
                     PGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            303..>545
                     /locus_tag="SDA06_002957"
     CDS             303..>545
                     /locus_tag="SDA06_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230143.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKN"
BASE COUNT          203 a           99 c           79 g          164 t
ORIGIN      
        1 cttaataatt taaatgaaat acatgattgg gctcaaaata atttacttaa tcgtaaacaa
       61 gctgccaaaa tcactggaca aaactatgat gctttttcta gctctttacg tagaaataat
      121 atcaaaccat ttctagaaat aggcgacgaa cctggtcctt ccatcattcg cttatatcta
      181 agagaagata ttgaaaaata cgcaaaacag ctcgaagaaa aaagacagaa acgtcaatga
      241 ggcgttttct gtcttggatt tacaagtaaa acaataataa acacttttac aacggaggtt
      301 ctatgaataa gaaatacatt gtttcagttg gttcaatcgt actgattgta ctcgctatta
      361 ttgctttttc tgtatccaac aaaacagaaa cacagccaca aaatacggtt caatcgacta
      421 ctacaagtca atctagcgat cattccgtct atccttcact aaatagttta aaagaaacgc
      481 atggacaaga tttaattgat cttggttctt ctcaaattac tactgtctta tttgaaaaga
      541 aaaat
//
LOCUS       DARDHD010000391          542 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000391 DARDHD010000000
VERSION     DARDHD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.411"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            118..516
                     /gene="spxA"
                     /locus_tag="SDA06_002958"
     CDS             118..516
                     /gene="spxA"
                     /locus_tag="SDA06_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4230144.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          189 a           86 c          110 g          157 t
ORIGIN      
        1 ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca gatgggagat
       61 tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg gtgcacgttg
      121 ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc gtggttgcaa
      181 gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa cagttctgaa
      241 ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc gacacgttcc
      301 aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga attattggaa
      361 cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga taaacgtctg
      421 caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt acgacaatta
      481 gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa acttggaaaa
      541 ga
//
LOCUS       DARDHD010000392          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000392 DARDHD010000000
VERSION     DARDHD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.413"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDA06_002959"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDA06_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG4230145.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SDA06_002960"
     CDS             complement(349..>526)
                     /locus_tag="SDA06_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230146.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARDHD010000393          520 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000393 DARDHD010000000
VERSION     DARDHD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.414"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>520
                     /locus_tag="SDA06_002961"
     CDS             <1..>520
                     /locus_tag="SDA06_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4230147.1"
                     /translation="KVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPDASG
                     EIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYL
                     VASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKDQEV
                     EAFPMSVCMSLYN"
BASE COUNT          195 a           54 c          122 g          149 t
ORIGIN      
        1 caaggtaagt gtgatagtac caatttacaa tgttgaaaaa tatttaagaa aatgtgtgga
       61 tagtttattg agtcagacat taaaagagat agaaatcgta ttagttgatg atggttcgcc
      121 agatgcttcg ggggaaatag cagatgagta tcaaaaaaaa tattctaatg taaaaacaat
      181 acatagagaa aatggggggt taggaccggc aagaaataca ggaattgaga atgcgacagg
      241 agaatatgtg gcttttcttg attcagatga ttgggtagaa agtgatatgt atgaaaaatt
      301 atacttagtt gcgtccaaag aaaatgctga tatagtagtt agtggacatt gtgattttgc
      361 gaatgacaaa gctatggtga aaaagcaaca tcctttgagt gggaatgtat atgatacgcc
      421 taatagtatt aaaaatgtac gaaaaaattt atttggacat agtccaaaag atcaagaagt
      481 agaggccttc ccgatgtcag tatgtatgtc tttgtataat
//
LOCUS       DARDHD010000394          515 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000394 DARDHD010000000
VERSION     DARDHD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.415"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SDA06_002962"
     CDS             132..344
                     /locus_tag="SDA06_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230148.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          171 a           82 c           96 g          166 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaat
//
LOCUS       DARDHD010000395          501 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000395 DARDHD010000000
VERSION     DARDHD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.416"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            201..>501
                     /locus_tag="SDA06_002963"
     CDS             201..>501
                     /locus_tag="SDA06_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230149.1"
                     /translation="MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSL
                     TLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLT"
BASE COUNT          160 a           80 c           66 g          195 t
ORIGIN      
        1 tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat
       61 ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac
      121 tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt
      181 ctttatttat agcctatacg atgagtatag tgatctatta tattttttct actaaaaata
      241 attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa
      301 gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg
      361 ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg
      421 atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt
      481 ttatgagtaa caaacttaca a
//
LOCUS       DARDHD010000396          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000396 DARDHD010000000
VERSION     DARDHD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.417"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SDA06_002964"
     CDS             complement(62..259)
                     /locus_tag="SDA06_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4230150.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARDHD010000397          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000397 DARDHD010000000
VERSION     DARDHD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.418"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(164..463)
                     /locus_tag="SDA06_002965"
     CDS             complement(164..463)
                     /locus_tag="SDA06_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4230151.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          153 a           88 c           62 g          182 t
ORIGIN      
        1 tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac atctgtttct
       61 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
      121 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      181 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      241 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      301 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      361 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      421 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      481 aatat
//
LOCUS       DARDHD010000398          458 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000398 DARDHD010000000
VERSION     DARDHD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.420"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>458
                     /gene="bsh"
                     /locus_tag="SDA06_002966"
     CDS             <1..>458
                     /gene="bsh"
                     /locus_tag="SDA06_002966"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile salt hydrolase"
                     /protein_id="HEG4230152.1"
                     /translation="YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNA
                     YSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCD
                     VGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISY"
BASE COUNT          153 a           63 c           83 g          159 t
ORIGIN      
        1 tatatgataa ccctgtgggc gttcttacca ataatccttc atttgactat caattattta
       61 atttaaacaa ttatcgtgtc ttatcgagtg aaactcctaa aaataatttt tcaaatcaaa
      121 taagtttgaa tgcctatagc cgcggtatgg gagggatagg cttgcctgga gatttatcct
      181 cagtatctcg ttttgttaaa gcgactttta cgaagctgaa ttctgtatct ggagattcag
      241 agtcagaaag tattagtcaa tttttccata tcttaggttc agtagaacaa caaaaaggtt
      301 tgtgtgatgt aggtgatgga aaatatgaat atacaattta ttcttcttgt tgcaatgttg
      361 acaaaggaat ctattattat cgaacatatg aagacagtca aattactgca attgatatga
      421 ataaagaaga cttagatagt cataagttaa ttagttat
//
LOCUS       DARDHD010000399          457 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000399 DARDHD010000000
VERSION     DARDHD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.421"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>457)
                     /locus_tag="SDA06_002967"
     CDS             complement(<1..>457)
                     /locus_tag="SDA06_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4230153.1"
                     /translation="ISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALRIL
                     LDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSES
                     QTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNS"
BASE COUNT          155 a           75 c           61 g          166 t
ORIGIN      
        1 gaatttctaa attcgttaaa ttgatgagag tctaattcag tggaaatatc aaataattgc
       61 tttctaatag ctttgttagg tctaataaat agagaaatac tacctacacc tttatctgca
      121 tttgaaatca attcttctaa gattgtttga ctttcagaat ttaaagaaat ggaagcacaa
      181 atctcattgt caaattcttc ttcgctgatt ccttcaaatg tcgcagagat aataatccaa
      241 tgcccttgcc acccattttg aattccatga aaaaaatctg attcttttag tgttttacta
      301 ctatagtaat aactatcatc taataagatt ctcaatgcag taagagcatt ggttttccct
      361 gaatcattct cacctataat agtatttgtc tcattatcga atcgaaagat ttcggattca
      421 taatttctaa aatttcgaat ttgaagagta gatataa
//
LOCUS       DARDHD010000400          444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000400 DARDHD010000000
VERSION     DARDHD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.422"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..>444)
                     /locus_tag="SDA06_002968"
     CDS             complement(37..>444)
                     /locus_tag="SDA06_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230154.1"
                     /translation="ELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQ
                     FIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNL
                     TKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           82 c           73 g          152 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actc
//
LOCUS       DARDHD010000401          415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000401 DARDHD010000000
VERSION     DARDHD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.423"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SDA06_002969"
     CDS             complement(<1..339)
                     /locus_tag="SDA06_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4230155.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARDHD010000402          407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000402 DARDHD010000000
VERSION     DARDHD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.424"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>407
                     /locus_tag="SDA06_002970"
     CDS             <1..>407
                     /locus_tag="SDA06_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG4230156.1"
                     /translation="FDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGIADY
                     PLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKKLL
                     KNINLANINYSDELPLSPLHWLLADKEKSIVIE"
BASE COUNT          145 a           53 c           74 g          135 t
ORIGIN      
        1 tttgattatg aaatatctta caatgaagta gttactgtta ctccaagaaa ttataagttg
       61 aattttcgaa aggtaaatga tttggatact cattatgcaa tgattggtat tgccgctggt
      121 atagctgact accctcttta ttacgatgcg acaaatgaaa aaggattgag tatggctggg
      181 ctaaattttt ctgggtatgc tgattataaa gaaatacaag aagggaaaga caatgtatct
      241 ccttttgaat ttattccttg gattttagga caatgctcaa cagtaggaga agctaaaaaa
      301 ttgttaaaaa atatcaattt agcaaatata aattatagtg acgaacttcc tttatcccct
      361 ttacattggc tattagctga taaagaaaaa tcaattgtca ttgaaag
//
LOCUS       DARDHD010000403          405 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000403 DARDHD010000000
VERSION     DARDHD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.426"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..345
                     /locus_tag="SDA06_002971"
                     /pseudo
     CDS             127..345
                     /locus_tag="SDA06_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          137 a           59 c           71 g          138 t
ORIGIN      
        1 caatatcgat cagttttgag gaaatgaaat tctagatatt ttttgctaca tagagttact
       61 ttcaatattt tttaacgtaa ttaggcagaa gtcactcatc ctctataggt ttgtgatgaa
      121 attgccattg atccatttaa gtaagagcaa aaaactggag gtttatcagg taattggtct
      181 aatggattta agtatgcggt caccgtgtat agagttgctt atgagattca agaaaataaa
      241 gttattccag tattgctata tggaacacat aacaattttt atgaattagc cgagaacact
      301 cgcaacttta ttcgtgtaga tgaatcggct tcggttgagt tataacttta gctatatcaa
      361 tcaaccgacc gacatatgtt aaatctatat ataataaaaa tacct
//
LOCUS       DARDHD010000404          402 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000404 DARDHD010000000
VERSION     DARDHD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.427"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..373
                     /locus_tag="SDA06_002972"
     CDS             <1..373
                     /locus_tag="SDA06_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4230157.1"
                     /translation="GYVEKPFSLPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTA
                     KINGEEIDVNAKELEVLKCLLDNNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELR
                     KKLQLDCITTIRNVGYKLERK"
BASE COUNT          178 a           33 c           72 g          119 t
ORIGIN      
        1 cggatatgta gaaaagccat tttcattgcc ggtcttaaaa gctagaatag attctttaat
       61 taagaagaat tatggacatt tagagaagtt tgaatataag gatctatcgg ttaattttaa
      121 tagctataca gctaaaataa atggtgaaga aatagatgta aatgcaaaag aactagaagt
      181 attaaaatgt ttattggata ataatggaca agtgttaaca agaatgcaaa ttattgatta
      241 tgtatggaaa gatagcgaag aaactcctta tgatcgagta atagatgtat atataaaaga
      301 acttagaaaa aaattacaat tagactgtat aactactata agaaatgtag gatataaatt
      361 ggagagaaaa tgaaaaaatt aaagatattt ccaaaaatgt tc
//
LOCUS       DARDHD010000405          400 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000405 DARDHD010000000
VERSION     DARDHD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.428"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          111 a           98 c           76 g          115 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgcccta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggattatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARDHD010000406          397 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000406 DARDHD010000000
VERSION     DARDHD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.430"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..212
                     /locus_tag="SDA06_002973"
     CDS             18..212
                     /locus_tag="SDA06_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230158.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          170 a           51 c           77 g           99 t
ORIGIN      
        1 agaaagaaga ggatcaaatg tattatgtag aagtaaaaac aaaaggcgtc aaaaacaaac
       61 aatatgtgaa aggtatcagt aacgaatatc ctttacttgg ttcatggaaa gaagctgctc
      121 ctttttccaa accatgtgcc ataaaaataa agaacgagct agaaaaagaa ctgacttgtg
      181 gtaaagctgt tgtagatatc atcgaaaagt aaatccaatg tatagaaagg aagatcacac
      241 atgaaaagcg acgtacaaat tgctaagatt atagaattag aagaagaaga cgcagtgttg
      301 acaattgaaa aaaaataaat gtttgtatac catatgatta tttcaatatg tatcctggaa
      361 ttggagatct cgtagagcta tatcaagatg aatcaaa
//
LOCUS       DARDHD010000407          375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000407 DARDHD010000000
VERSION     DARDHD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.435"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SDA06_002974"
     CDS             complement(<1..132)
                     /locus_tag="SDA06_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4230159.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DARDHD010000408          369 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000408 DARDHD010000000
VERSION     DARDHD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.437"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..317)
                     /locus_tag="SDA06_002975"
     CDS             complement(<1..317)
                     /locus_tag="SDA06_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4230160.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASAS"
BASE COUNT          118 a           80 c           48 g          123 t
ORIGIN      
        1 gaagctgatg ccattgatgt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc
       61 atccgtaaaa tccacggttt aaatttgcca gttttagatg gttttgatcc atccacaata
      121 cgccccattg ttatgtctgt aactgcatca ataaatcttg ctactaaaaa taatgttcct
      181 acggtagctc cgcttatccc caatactttt gtataaaaaa tcattaaaaa agaactagca
      241 aaaatgaaag tgaaatcatt cccaaaatct ccaaacatat atcctatttt gtctcttaga
      301 ccaaattttc tttccatata atttgcgctc cttcctattg ttaagcgttt tcattctgtt
      361 gaactgcca
//
LOCUS       DARDHD010000409          359 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000409 DARDHD010000000
VERSION     DARDHD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.439"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..281)
                     /locus_tag="SDA06_002976"
                     /pseudo
     CDS             complement(1..281)
                     /locus_tag="SDA06_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
BASE COUNT          131 a           61 c           43 g          124 t
ORIGIN      
        1 tttaaaaaat accttttaaa tttcttatag tcctctgaaa tcaaaatata gcgtgatgga
       61 tactcatcaa aagaagaatc gtattcttcc tttaaaattc ccgatttact taatttttct
      121 agtacatcaa atactctatc actcaagtaa ctccttaaat tgttatcact ttcccattga
      181 actacttttc tataagttga attatcagtt gaaaagatag aattttcatc cataaacgca
      241 tatattagca gcaatctttc atcattgtca aattctattg gatgaatcgg ctacactaga
      301 ctagacagaa aaaataaggt gtgtagaata ataaaaaaca cactggagga tttttcatg
//
LOCUS       DARDHD010000410          358 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000410 DARDHD010000000
VERSION     DARDHD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.440"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>358)
                     /locus_tag="SDA06_002977"
     CDS             complement(<1..>358)
                     /locus_tag="SDA06_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4230161.1"
                     /translation="VNFRALFYIFSGLFSHILGFRLETNLRKKGISGLTEASFRFFDL
                     NSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGLVLIAMLV
                     IGVFLLKKMTENTNFIQ"
BASE COUNT          135 a           64 c           52 g          107 t
ORIGIN      
        1 ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat
       61 ggcgattaga accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac
      121 aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt
      181 agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg
      241 aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa
      301 cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctctaa agttaacc
//
LOCUS       DARDHD010000411          354 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000411 DARDHD010000000
VERSION     DARDHD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.441"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          100 a           67 c           47 g          140 t
ORIGIN      
        1 tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg
       61 tctctagagt aactacgcct aatattacga ggcatcatat taatctatca gcactagtac
      121 ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc
      181 taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac
      241 gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa
      301 tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DARDHD010000412          353 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000412 DARDHD010000000
VERSION     DARDHD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.442"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>353
                     /locus_tag="SDA06_002978"
     CDS             <1..>353
                     /locus_tag="SDA06_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG4230162.1"
                     /translation="QEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDA
                     DGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQ"
BASE COUNT          122 a           66 c           72 g           93 t
ORIGIN      
        1 caagagtaca gcaaagtcct ctattatctt tggaagaaga aaaatagaca atccttctat
       61 tcatggaaaa tggacgaaac ctatatcaaa attaagggac gttggcatta tctttatcgt
      121 gcaattgatg cggacggctt aaccttagat atctggttac gaaagaaacg ggatacgcaa
      181 gcagcctatg ctttcttaaa acgactccat aaacagtttg gtgagccgaa agcaattgtg
      241 accgataaag caccttctct tggctccgcc tttagaaagt tacagagtgt gggtttatat
      301 actaagacag agcaccgaac tgtgaagtat cttaacaatt taatagaaca aga
//
LOCUS       DARDHD010000413          348 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000413 DARDHD010000000
VERSION     DARDHD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.443"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>348
                     /locus_tag="SDA06_002979"
     CDS             <1..>348
                     /locus_tag="SDA06_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4230163.1"
                     /translation="EFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMD"
BASE COUNT          107 a           69 c           63 g          109 t
ORIGIN      
        1 tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa gtgccgtcat
       61 tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg gccgtaaggc
      121 attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa acttctttaa
      181 atctattacg tttgactgtg gaaaagaatt ttctaactgg aaagccatta gtaaccaaca
      241 tgatattgat atatattttg cggaccctgg aacaccttct caacgcccat taaacgagaa
      301 ttctaacggg attctgcgtc gtaatggact gccgaaatca atggattt
//
LOCUS       DARDHD010000414          345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000414 DARDHD010000000
VERSION     DARDHD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.444"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..321)
                     /locus_tag="SDA06_002980"
     CDS             complement(<1..321)
                     /locus_tag="SDA06_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4230164.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DARDHD010000415          344 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000415 DARDHD010000000
VERSION     DARDHD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.445"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..93
                     /locus_tag="SDA06_002981"
     CDS             <1..93
                     /locus_tag="SDA06_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4230165.1"
                     /translation="KYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            94..>344
                     /locus_tag="SDA06_002982"
     CDS             94..>344
                     /locus_tag="SDA06_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4230166.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDT"
BASE COUNT          133 a           45 c           67 g           99 t
ORIGIN      
        1 aaatatttct ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt atatcatgca
       61 aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa ttttatttac tatttgtggt
      121 agagctgggt ctaaggggtt taaaaataaa aatttgaaat atttggcagg aaagccccta
      181 gtccatttcg ctttagcaat gatagatatc tatcaacaaa aacatcctga acataattgc
      241 acgacttgtt tgaatacgga cagtaatgag ttagtagaat tgacgactaa gttaaatccg
      301 tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag atac
//
LOCUS       DARDHD010000416          344 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000416 DARDHD010000000
VERSION     DARDHD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.446"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            76..267
                     /locus_tag="SDA06_002983"
     CDS             76..267
                     /locus_tag="SDA06_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4230167.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          123 a           49 c           60 g          112 t
ORIGIN      
        1 ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg
       61 gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat
      121 gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg
      181 agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct
      241 atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa
      301 gatagtttca aatttcgaac aataaatttg aaactatctt cttt
//
LOCUS       DARDHD010000417          341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000417 DARDHD010000000
VERSION     DARDHD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.447"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..243)
                     /locus_tag="SDA06_002984"
     CDS             complement(<1..243)
                     /locus_tag="SDA06_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4230168.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIE"
BASE COUNT           97 a           79 c           37 g          128 t
ORIGIN      
        1 ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat
       61 aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat
      121 tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg
      181 aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt
      241 catttataac acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa
      301 ccttttctca ttatcctgcc cgttagttga ataagcaatt t
//
LOCUS       DARDHD010000418          340 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000418 DARDHD010000000
VERSION     DARDHD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.448"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            64..>340
                     /locus_tag="SDA06_002985"
     CDS             64..>340
                     /locus_tag="SDA06_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG4230169.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          111 a           62 c           65 g          102 t
ORIGIN      
        1 tagaaaagac accctgaaat cctttagaat taatttggct accaattcat aggaggattc
       61 aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct aaatatctat
      121 ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt gatttacaaa
      181 ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg
      241 atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc
      301 acttatctgg agctgaagaa acaacgattt aaatgtaagt
//
LOCUS       DARDHD010000419          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000419 DARDHD010000000
VERSION     DARDHD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.449"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SDA06_002986"
                     /pseudo
     CDS             1..338
                     /locus_tag="SDA06_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARDHD010000420          336 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000420 DARDHD010000000
VERSION     DARDHD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.450"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..220)
                     /locus_tag="SDA06_002987"
     CDS             complement(<1..220)
                     /locus_tag="SDA06_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230170.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLI"
BASE COUNT          129 a           58 c           40 g          109 t
ORIGIN      
        1 caatcaaata taaactaact atacttgtta gaacaaaaaa acaggcgttg acttgttctt
       61 gattagttaa aaatatcaat aaaggaatac ttacccaata tatttttcta tttatttctt
      121 ctcctcttaa gagttgaaaa aacggataaa aagttagtaa actaaatgct actggccact
      181 gatagttcaa ggtggttgct ctccaaccgg tttcccccat tactgtcaat ggtattaaac
      241 agtatataga gcaaaccaat aataaatttt taacatttat tttctcaaaa acatacttgc
      301 aaagtaatac tacaattcct aacataatcg tagaat
//
LOCUS       DARDHD010000421          330 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000421 DARDHD010000000
VERSION     DARDHD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.451"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(77..>330)
                     /locus_tag="SDA06_002988"
     CDS             complement(77..>330)
                     /locus_tag="SDA06_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4230171.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT           94 a           59 c           52 g          125 t
ORIGIN      
        1 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactct ttttttcatg
       61 ctctaaaatg atatttttac acaaccgtaa cggttgtttt acgaaattgg acgggacttt
      121 gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag ctatcgatgg
      181 ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct tgttttaata
      241 agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga gacatgcttt
      301 gaaagatttt gtactccttc aatttgtttc
//
LOCUS       DARDHD010000422          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000422 DARDHD010000000
VERSION     DARDHD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.454"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SDA06_002989"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SDA06_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARDHD010000423          320 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000423 DARDHD010000000
VERSION     DARDHD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.455"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>320)
                     /locus_tag="SDA06_002990"
     CDS             complement(<1..>320)
                     /locus_tag="SDA06_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4230172.1"
                     /translation="FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGT
                     YSKDNIRRFLTKKVNYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNL
                     DIFD"
BASE COUNT           99 a           58 c           39 g          124 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag
//
LOCUS       DARDHD010000424          311 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000424 DARDHD010000000
VERSION     DARDHD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.456"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          113 a           58 c           52 g           88 t
ORIGIN      
        1 aaatacaatt tttgaataac gatccctgct attcgagcta gaaactcaag tacgccatga
       61 cctgattagc gacaaatact tagagatttc tttttgttct gggaaaacaa aatgccatga
      121 caatagcagc aaataatgat acttccgtaa ggctcgccgt aaactgggga gaggtgaaat
      181 tcctatgtgc ttacccaaag caattcaaaa atttcataac tactatttta ggagatgatt
      241 ttatagaatc cagagtctac ctgaataaaa aacaaacctg tgcatttcta ggaatcgata
      301 ataatactct a
//
LOCUS       DARDHD010000425          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000425 DARDHD010000000
VERSION     DARDHD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.457"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SDA06_002991"
     CDS             complement(76..>308)
                     /locus_tag="SDA06_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4230173.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARDHD010000426          295 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000426 DARDHD010000000
VERSION     DARDHD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.462"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..104
                     /locus_tag="SDA06_002992"
     CDS             <1..104
                     /locus_tag="SDA06_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG4230174.1"
                     /translation="CNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGGR"
     gene            104..>295
                     /locus_tag="SDA06_002993"
     CDS             104..>295
                     /locus_tag="SDA06_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4230175.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIV"
BASE COUNT          117 a           32 c           53 g           93 t
ORIGIN      
        1 attgtaacaa agaaaaatgt tgggtaaatt ggggacaatc atggtcagaa gggttaaaac
       61 gttggggaga taatcttttt ggtagtttta gtggaggacg ataatgactt caataaacat
      121 tgatcacaca aaaaaagtca tacatgaatt atacaatagt ttaatgacac gacctaatca
      181 gacgaaagca ttattagata ttactgatgt tttatttcag gtatataaaa aaattgatac
      241 agcaaaatat cctgaaattc ttgtaaataa attagttaat tatatttaca tcgtg
//
LOCUS       DARDHD010000427          289 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000427 DARDHD010000000
VERSION     DARDHD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.463"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(56..217)
                     /locus_tag="SDA06_002994"
     CDS             complement(56..217)
                     /locus_tag="SDA06_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG4230176.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT           90 a           63 c           31 g          105 t
ORIGIN      
        1 tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact cctctctatt
       61 ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt ccacatctga
      121 tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa acttttttac
      181 attttccaca attaatacac ttatccttat cctgaatcgg ctacactaga ctagacagaa
      241 aaaataaggt gtgtagaata ataaaaaaca cactggagga tttttcatg
//
LOCUS       DARDHD010000428          284 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000428 DARDHD010000000
VERSION     DARDHD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.466"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..124)
                     /locus_tag="SDA06_002995"
                     /pseudo
     CDS             complement(14..124)
                     /locus_tag="SDA06_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          107 a           36 c           45 g           96 t
ORIGIN      
        1 tctgttcatt tttttatttt attagtaatg attccccatg ccaaatcatg atccgtaatt
       61 taaagttata aaagcttcga aatccattgg catttcgttg gaataccttg atatgattat
      121 tcaaatataa aatatagaga gaagagagag agggaagata tttataaagg gcaaagttat
      181 gtttactcaa cttaggaaat agaaatagat caatttatat tctcttgtca atcgaatatg
      241 aaccttagca ctatatacca aatatagtaa aatgctgaaa gatg
//
LOCUS       DARDHD010000429          282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000429 DARDHD010000000
VERSION     DARDHD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.467"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SDA06_002996"
     CDS             <1..>282
                     /locus_tag="SDA06_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG4230177.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARDHD010000430          278 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000430 DARDHD010000000
VERSION     DARDHD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.469"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..>278
                     /locus_tag="SDA06_002997"
     CDS             2..>278
                     /locus_tag="SDA06_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4230178.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          107 a           49 c           50 g           72 t
ORIGIN      
        1 aatgacctac aaacatctta ccatagacga actgacaatg atagaatcat attatcttca
       61 acataataag ccggttgaaa tcgctaaccg aatgggacgt gctatacaaa ctatttataa
      121 tgtagtcaat aagttcaagc aaggcaagac tgctcttgat tattggcacc agtataaaga
      181 aaataagaaa aaatgtggta gaaaagtcat tcaattacct gctcatgaag tagattacat
      241 taaagagaaa gtcactcttg gttggacgcc tgacgtca
//
LOCUS       DARDHD010000431          276 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000431 DARDHD010000000
VERSION     DARDHD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.470"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..252)
                     /locus_tag="SDA06_002998"
     CDS             complement(34..252)
                     /locus_tag="SDA06_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230179.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT           95 a           57 c           42 g           82 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatg
//
LOCUS       DARDHD010000432          275 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000432 DARDHD010000000
VERSION     DARDHD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.471"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(156..>275)
                     /locus_tag="SDA06_002999"
     CDS             complement(156..>275)
                     /locus_tag="SDA06_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG4230180.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT           96 a           50 c           46 g           83 t
ORIGIN      
        1 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
       61 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
      121 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
      181 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
      241 tcttggtttg cagcagagta gtcaccattt aagta
//
LOCUS       DARDHD010000433          270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000433 DARDHD010000000
VERSION     DARDHD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.473"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>270
                     /locus_tag="SDA06_003000"
     CDS             <1..>270
                     /locus_tag="SDA06_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4230181.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARDHD010000434          239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000434 DARDHD010000000
VERSION     DARDHD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.481"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>239
                     /locus_tag="SDA06_003001"
     CDS             <1..>239
                     /locus_tag="SDA06_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4230182.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DARDHD010000435          231 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.486, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000435 DARDHD010000000
VERSION     DARDHD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.486"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>231
                     /locus_tag="SDA06_003002"
     CDS             <1..>231
                     /locus_tag="SDA06_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG4230183.1"
                     /translation="LLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATI
                     ATVICSYVIYVVRKRAVGKDIFIKHYHIVVESW"
BASE COUNT           70 a           29 c           51 g           81 t
ORIGIN      
        1 ctgttaggac ctatcttatc agcaaaaaaa gattctaaat ccatgatgtg gtctgcaatc
       61 ataggggcat tagttaatgt gattatgagt gtagtccttg tttataagat aggggttcaa
      121 ggtgccacaa tagccactgt tatatgttca tatgtaatat atgttgttcg taaacgagct
      181 gtgggaaaag atatttttat aaagcattat catattgttg tggaatcttg g
//
LOCUS       DARDHD010000436          227 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.denovo.487, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000436 DARDHD010000000
VERSION     DARDHD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.denovo.487"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="SDA06_003003"
     rRNA            complement(<1..>227)
                     /locus_tag="SDA06_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DARDHD010000437         5865 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.146, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000437 DARDHD010000000
VERSION     DARDHD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.146"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            201..1097
                     /locus_tag="SDA06_003004"
     CDS             201..1097
                     /locus_tag="SDA06_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4230184.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1247..2188)
                     /locus_tag="SDA06_003005"
     CDS             complement(1247..2188)
                     /locus_tag="SDA06_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4230185.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2314..3435
                     /locus_tag="SDA06_003006"
     CDS             2314..3435
                     /locus_tag="SDA06_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4230186.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3693..5195
                     /gene="eat(A)"
                     /locus_tag="SDA06_003007"
     CDS             3693..5195
                     /gene="eat(A)"
                     /locus_tag="SDA06_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4230187.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5223..5789
                     /locus_tag="SDA06_003008"
     CDS             5223..5789
                     /locus_tag="SDA06_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4230188.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1925 a         1035 c         1229 g         1676 t
ORIGIN      
        1 gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt aggcgaattt
       61 taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt tcatacaaat
      121 ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
      181 gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca aaaaataaat
      241 cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
      301 agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg atcgtttttt
      361 tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc aatgcaaaaa
      421 ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg aagcaggcac
      481 aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca aaagaacttg
      541 ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct agcgaaccaa
      601 agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca gaaagctata
      661 agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca catcgaggga
      721 atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg gaatcgcgcg
      781 ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca tttgaatcca
      841 tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat catttttatc
      901 gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg gaatttctgc
      961 atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca gaaaattggg
     1021 caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat gacctctcct
     1081 ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc
     1141 acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa
     1201 aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa
     1261 ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat
     1321 catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat
     1381 ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat
     1441 aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg
     1501 cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc
     1561 ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac
     1621 aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt
     1681 cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta
     1741 gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc
     1801 acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt acatcttcat
     1861 gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg
     1921 aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt
     1981 cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac
     2041 cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat
     2101 cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg
     2161 ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct
     2221 tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa
     2281 gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca
     2341 tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat
     2401 cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca
     2461 atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt
     2521 cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa
     2581 tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt
     2641 gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa
     2701 attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc
     2761 ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt
     2821 cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt
     2881 accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga
     2941 atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa
     3001 gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt
     3061 ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc
     3121 actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc
     3181 gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat
     3241 cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat
     3301 tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa
     3361 cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc
     3421 atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg
     3481 acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac
     3541 tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg
     3601 atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat
     3661 aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga
     3721 cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca
     3781 cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata
     3841 ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt
     3901 tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg
     3961 attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct
     4021 tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt
     4081 tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct
     4141 ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca
     4201 gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc
     4261 aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg
     4321 aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag
     4381 cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg
     4441 gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac
     4501 ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag
     4561 aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc
     4621 accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg
     4681 agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg
     4741 caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag gttataggag
     4801 aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc
     4861 gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca
     4921 acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta
     4981 tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata
     5041 catgggatga accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga
     5101 tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag
     5161 tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag
     5221 gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac
     5281 acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga
     5341 ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc
     5401 caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg
     5461 ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg
     5521 attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg
     5581 tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga
     5641 aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa
     5701 gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag
     5761 ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt
     5821 tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgac
//
LOCUS       DARDHD010000438         5799 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.148, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000438 DARDHD010000000
VERSION     DARDHD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.148"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            318..782
                     /locus_tag="SDA06_003009"
     CDS             318..782
                     /locus_tag="SDA06_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4230189.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            766..1395
                     /locus_tag="SDA06_003010"
     CDS             766..1395
                     /locus_tag="SDA06_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4230190.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1749..3227
                     /gene="msr(C)"
                     /locus_tag="SDA06_003011"
     CDS             1749..3227
                     /gene="msr(C)"
                     /locus_tag="SDA06_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4230191.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3274..3720
                     /locus_tag="SDA06_003012"
     CDS             3274..3720
                     /locus_tag="SDA06_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG4230192.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3919..4434)
                     /locus_tag="SDA06_003013"
     CDS             complement(3919..4434)
                     /locus_tag="SDA06_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4230193.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4551..5753
                     /locus_tag="SDA06_003014"
     CDS             4551..5753
                     /locus_tag="SDA06_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4230194.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1796 a          966 c         1229 g         1808 t
ORIGIN      
        1 tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg
       61 aaatttttgg cggtgtaaaa ttatctactg cagaagagct atcttctata gtttgataag
      121 aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt tcattctaca
      181 aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac acagctgatt
      241 ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac gagtcaatta
      301 ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat caaatcaaat
      361 tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt cttactagat
      421 acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt ttgcaagaaa
      481 agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa gcaaatggct
      541 atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg ctaacagaaa
      601 aagcccagaa gaaaatcaag cattgtgaaa tggaccataa agatacaggt tccgtgctat
      661 ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat aaattgaata
      721 cccaactttg caatataaca acagaaacga aagaggttaa taataatgga acaaaaaaat
      781 gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga aacatacaaa
      841 atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc
      901 aatatgaaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt
      961 cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt catgattttt
     1021 ggcgatatgc aatgttacga atatggcgaa gaaatctaca accaagctta cgaatcaggg
     1081 aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta ttacaagagt
     1141 ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa
     1201 ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg attcgaagca
     1261 gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt tattatttta
     1321 tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc agaaaagatc
     1381 actatgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt ttttgaatcg
     1441 tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt
     1501 tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg
     1561 gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg
     1621 gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca
     1681 aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg
     1741 taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc
     1801 agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag
     1861 gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg
     1921 atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg
     1981 ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa
     2041 cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt
     2101 cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta
     2161 ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg
     2221 atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc
     2281 gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc
     2341 agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag
     2401 aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa
     2461 tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa
     2521 agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc
     2581 agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca
     2641 tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt
     2701 ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa
     2761 ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct
     2821 ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg
     2881 tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt
     2941 ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc
     3001 tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta
     3061 attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa
     3121 cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg
     3181 aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg
     3241 attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata
     3301 atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg
     3361 ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg
     3421 gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta
     3481 ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta
     3541 ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa
     3601 ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag
     3661 ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga
     3721 tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc
     3781 agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt
     3841 cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag
     3901 aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca
     3961 ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa
     4021 ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt
     4081 gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga
     4141 aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt
     4201 gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga
     4261 gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt
     4321 atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc
     4381 agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat
     4441 cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg
     4501 catattgaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat
     4561 tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag
     4621 cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa
     4681 tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta
     4741 ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga
     4801 tcggtttgaa aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg
     4861 gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga
     4921 atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag
     4981 aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag
     5041 tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga
     5101 tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa
     5161 aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt tcagttggac
     5221 taatcgcatt gttatataat ttcggattct ttactttact tgcgtatgcg ccatttttat
     5281 tgattggcta ttctgaatta gaagtcggtt ttgtattttt tggctggggt gtgcttctgg
     5341 ctattgcttc gattttcgtt gcaccggctc ttgagcggaa gtttacgact tatcggacaa
     5401 ttttagcagc atttgtctta ttcattattt gtttgctgtt attaggagct ggaacttcta
     5461 tgccagtcct tgtaccgatc agcattgtgt tagcaggatt tttccaagga attatcaata
     5521 cactgttgac gacgattgct atggaaatac caggtctgca acgaaacgtt gcttcgtcca
     5581 gctatagttt tgtccgcttt ttcggtggag ctttagcacc atttattgca ggtgtgattg
     5641 gtgaacatat tggtgaaagc tatgcgtttt attttgcagg tgccattgtt cttatcagtg
     5701 tatgttgtat catttatcac cgtaattatt ttgctacaaa tgaagaagaa taagaaatca
     5761 gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DARDHD010000439         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.153, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000439 DARDHD010000000
VERSION     DARDHD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.153"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SDA06_003015"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SDA06_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SDA06_003016"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SDA06_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4230195.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SDA06_003017"
     CDS             1851..3005
                     /locus_tag="SDA06_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4230196.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SDA06_003018"
     CDS             3220..4188
                     /locus_tag="SDA06_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4230197.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SDA06_003019"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SDA06_003019"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4230198.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SDA06_003020"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SDA06_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARDHD010000440         1882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.266, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000440 DARDHD010000000
VERSION     DARDHD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.266"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(615..905)
                     /locus_tag="SDA06_003021"
     CDS             complement(615..905)
                     /locus_tag="SDA06_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4230199.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1113..1805)
                     /locus_tag="SDA06_003022"
     CDS             complement(1113..1805)
                     /locus_tag="SDA06_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4230200.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          508 a          364 c          294 g          716 t
ORIGIN      
        1 ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc
       61 agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa
      121 actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt
      181 tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg
      241 cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg
      301 tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt
      361 ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc
      421 tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa
      481 atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg
      541 gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct
      601 cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct tgggtataga
      661 cttttctcat attctaccac aattttgggt ttccctaaca catgatagga tctcacaaca
      721 aattgctttc tattattata ataacctgta acgactaact tcatcccttc gtctacttca
      781 gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa actgaaacgg
      841 atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc atattttttc
      901 tccataacct tcacctcaat accattatac gaacatacat tccctttgta aagaagaaac
      961 tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat tggcacttcc
     1021 tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta ctgttgcttc
     1081 tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc ttcaacaatt
     1141 tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa cttttgaata
     1201 tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt
     1261 aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg attcaaacca
     1321 tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa
     1381 tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag tttgacattg
     1441 atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact ttctaagttt
     1501 tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt tgtttggtat
     1561 tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag tcgagcggta
     1621 agatctgtaa gcgcttgctc tttatctgtc aatgtatgat caagctgtgt cagattctca
     1681 ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg
     1741 cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat aaatttcttt
     1801 ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt gatctcaaac
     1861 acaatgtcaa gcgtttttaa at
//
LOCUS       DARDHD010000441         1508 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.289, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000441 DARDHD010000000
VERSION     DARDHD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.289"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..57
                     /locus_tag="SDA06_003023"
     CDS             <1..57
                     /locus_tag="SDA06_003023"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG4230201.1"
                     /translation="GTFSIFVINKVRYQPNQN"
     gene            115..846
                     /gene="erm(A)"
                     /locus_tag="SDA06_003024"
     CDS             115..846
                     /gene="erm(A)"
                     /locus_tag="SDA06_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4230202.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(972..>1508)
                     /locus_tag="SDA06_003025"
     CDS             complement(972..>1508)
                     /locus_tag="SDA06_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HEG4230203.1"
                     /translation="VRPLEVTVINRSEVVPWQYPPKREFIYGEWLRGEFENGQIQEPS
                     YDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIRRAIKDSLPELIEGIKGDERNV
                     ILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLLDIARKGYRGECDDKWEGLYS
                     KVKALVKYMKNSIETSLN"
BASE COUNT          528 a          273 c          235 g          472 t
ORIGIN      
        1 ggtacttttt ctatatttgt tattaataaa gttcgttatc aaccaaatca aaattaattg
       61 gttataatga acgcttaatg tcagttcatt ataaccagta aggagaaggt tataatgaac
      121 cagaaaaacc ctaaagacac gcaaaatttt attacttcta aaaagcatgt aaaagaaata
      181 ttgaatcaca cgaatatcag taaacaagac aacgtaatag aaatcggatc aggaaaagga
      241 cattttacca aagagctagt caaaatgagt cgatcagtta ctgctataga aattgatgga
      301 ggcttatgtc aagtgactaa agaagcggta aacccctctg agaatataaa agtgattcaa
      361 acggatattc taaaattttc cttcccaaaa catataaact ataagatata tggtaatatt
      421 ccttataaca tcagtacgga tattgtcaaa agaattacct ttgaaagtca ggctaaatat
      481 agctatctta tcgttgagaa gggatttgcg aaaagattgc aaaatctgca acgagctttg
      541 ggtttactat taatggtgga gatggatata aaaatgctca aaaaagtacc accactatat
      601 tttcatccta agccaagtgt agactctgta ttgattgttc ttgaacgaca tcaaccattg
      661 atttcaaaga aggactacaa aaagtatcga tcttttgttt ataagtgggt aaaccgtgaa
      721 tatcgtgttc ttttcactaa aaaccaattc cgacaggctt tgaagcatgc aaatgtcact
      781 aatattaata aactatcgaa ggaacaattt ctttctattt tcaatagtta caaattgttt
      841 cactaaatta aagtaataaa gcgttctcta atttcacaag aggacgcttt attcttccca
      901 aaaattgttc aatatttatc aataaatcag tagttttaaa agtaagcacc tgttattgca
      961 ataaaattag cctaattgag agaagtttct atagaatttt tcatatactt aacgagtgct
     1021 ttcacctttg aatatagtcc ttcccactta tcatcacact ctccccgata gccttttcta
     1081 gctatatcca gtaaagttac atgctcttta ggtaaaagag gtatagccca ttctgcagcg
     1141 acatctttcg aggtaatttc accagtagtc actgtttgcc acattcgagc tagggttaaa
     1201 attacattac gctcatcacc ttttatcccc tcaattagtt ctggcaaaga atccttaatt
     1261 gctcttcgaa tatctgtcaa aggtacggag acaagtatac ttgaagaatc aggaccaaat
     1321 agagaaatac tattctttct tgcttgtgct aaaacaatag ccaaatcagg atcatagctt
     1381 ggttcctgaa tttgtccatt ctcaaattca cccctgagcc actcaccgta tataaattct
     1441 ctttttggag gatattgcca agggacaact tcactcctat ttataaccgt aacttcaagt
     1501 ggtctaac
//
LOCUS       DARDHD010000442         1453 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.295, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000442 DARDHD010000000
VERSION     DARDHD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.295"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            188..271
                     /locus_tag="SDA06_003026"
                     /pseudo
     CDS             188..271
                     /locus_tag="SDA06_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            320..403
                     /locus_tag="SDA06_003027"
     CDS             320..403
                     /locus_tag="SDA06_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4230204.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            528..1265
                     /gene="erm(B)"
                     /locus_tag="SDA06_003028"
     CDS             528..1265
                     /gene="erm(B)"
                     /locus_tag="SDA06_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4230205.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          530 a          224 c          241 g          458 t
ORIGIN      
        1 tattgtgagt tattagtggt acagttttca accgttttaa ttataaaaaa gtggtgcatt
       61 tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct atgggtttaa
      121 catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct tgcgtatggt
      181 taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca
      241 ttatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat
      301 taagaaggag ggattcgtca tgttggtatt ccaaatgcgt tatgtagata aaacatctac
      361 tgttttgaaa cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc
      421 ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt
      481 taacttctgt atttgtttat agatgtaatc acttcaggag tgataacatg aacaaaaata
      541 taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat
      601 tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga
      661 cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt catctattca
      721 acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac caagatattc
      781 tacagtttca attccctaac aaacagaggt ataaaattgt tgggaatatt ccttaccatt
      841 taagcacaca aattattaaa aaagtggttt ttgaaagccg tgcgtctgac atctatctga
      901 ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct
      961 tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta
     1021 aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata
     1081 aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac
     1141 tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta
     1201 ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga
     1261 aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa agggaatgta
     1321 gataaattat taggtatagg gcacctctaa taactaccaa ttaattcacc tcaaaatgaa
     1381 attagaaaaa aacagcgaaa tttttaaatc tatttcttat cgatacaaat tcctcgtagg
     1441 cgctcgggac ccc
//
LOCUS       DARDHD010000443         1350 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.307, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000443 DARDHD010000000
VERSION     DARDHD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.307"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SDA06_003029"
                     /pseudo
     CDS             47..298
                     /locus_tag="SDA06_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SDA06_003030"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SDA06_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4230206.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARDHD010000444         1221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.317, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000444 DARDHD010000000
VERSION     DARDHD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.317"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1209
                     /locus_tag="SDA06_003031"
     CDS             169..1209
                     /locus_tag="SDA06_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4230207.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          405 a          190 c          243 g          383 t
ORIGIN      
        1 ttggtatgta agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt
       61 actccaaaac tgaactactt acttgcatat atacgccttt atagtggagt actacattta
      121 cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca
      181 ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa
      241 aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa
      301 aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag
      361 agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat
      421 tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
      481 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
      541 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
      601 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
      661 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt
      721 atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt
      781 aaaatctgtt ttaagtacgg tttatactat agccctgtta gtactccagc agattttcgg
      841 atgcttactc ctattgtact ggagcaagcc tttagaaaag ctggaacaga attgttagag
      901 ccatatctta gttttaaagt ttatgcacca caggaatatc tttcacgggc atataacgat
      961 gctcccaaat attgtgcaaa tatcgtaaat actcaactga aaaataatga ggtcattatt
     1021 attggagaaa ttcctgctcg atgtattcaa gattatcgca atgatttaac tttttttaca
     1081 aatgggctta gtgtttgttt agcagagcta aaaggatatc aggttaccac tggcgaacct
     1141 gtttgccaga cccgtcgtct aaatagtcgg atagataaag taagatatat gttcaataaa
     1201 ataacttagt gcgttttatg t
//
LOCUS       DARDHD010000445         1194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.319, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000445 DARDHD010000000
VERSION     DARDHD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.319"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="SDA06_003032"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="SDA06_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG4230208.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DARDHD010000446         1023 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.343, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000446 DARDHD010000000
VERSION     DARDHD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.343"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            64..846
                     /locus_tag="SDA06_003033"
     CDS             64..846
                     /locus_tag="SDA06_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4230209.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          359 a          142 c          208 g          314 t
ORIGIN      
        1 gggtaagtaa attgagtatc aatataaact ttatatgaac ataatcagtt acgaggtgaa
       61 atcatgagca atttgattaa cggaaaaata ccaaatcaag cgattcaaac attaaaaatc
      121 gtaaaagatt tatttggaag ttcaatagtt ggagtatatc tatttggttc agcagtaaat
      181 ggtggtttac gcattaacag cgatgtagat gttctagtcg tcgtgaatca tagtttacct
      241 caattaactc gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa gattggaaat
      301 acggattctg ttagaccact tgaagttacg gttataaata ggagtgaagt tgtcccttgg
      361 caatatcctc caaaaagaga atttatatac ggtgagtggc tcaggggtga atttgagaat
      421 ggacaaattc aggaaccaag ctatgatcct gatttggcta ttgttttagc acaagcaaga
      481 aagaatagta tttctctatt tggtcctgat tcttcaagta tacttgtctc cgtacctttg
      541 acagatattc gaagagcaat taaggattct ttgccagaac taattgaggg gataaaaggt
      601 gatgagcgta atgtaatttt aaccctagct cgaatgtggc aaacagtgac tactggtgaa
      661 attacctcga aagatgtcgc tgcagaatgg gctatacctc ttttacctaa agagcatgta
      721 actttactgg atatagctag aaaaggctat cggggagagt gtgatgataa gtgggaagga
      781 ctatattcaa aggtgaaagc actcgttaag tatatgaaaa attctataga aacttctctc
      841 aattaggcta attttattgc aataacaggt gcttactttt aaaactactg atttattgat
      901 aaatattgaa caatttttgg gaagaataaa gcgtcctctt gtgaaattag agaacgcttt
      961 attactttaa tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa ttgttccttc
     1021 gat
//
LOCUS       DARDHD010000447          947 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3624
            SAMEA114569746-rid20117883.guided.351, whole genome shotgun
            sequence.
ACCESSION   DARDHD010000447 DARDHD010000000
VERSION     DARDHD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569746
            Sequence Read Archive: ERR12203570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973526.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 23:35:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,034
            CDSs (total)                      :: 2,973
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 34 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569746-rid20117883.guided.351"
                     /isolate="VRE3624"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            232..780
                     /gene="aac(6')"
                     /locus_tag="SDA06_003034"
     CDS             232..780
                     /gene="aac(6')"
                     /locus_tag="SDA06_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4230210.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          334 a          142 c          202 g          269 t
ORIGIN      
        1 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
       61 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
      121 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
      181 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
      241 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
      301 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
      361 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
      421 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
      481 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
      541 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
      601 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
      661 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
      721 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
      781 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
      841 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
      901 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaa
//
